Veterinary Medicine Commons^™

Comparative Characterization Of Four Mouse Parthenogenetic Embryonic Stem (Pes) Cell Lines, Sasitorn Rungarunlert, Ruttachuk Rungsiwiwut, Sirilada Suphankong, Sasithorn Panasophonkul, Kamthorn Pruksananonda, Pramuan Virutamasen, Melinda K. Pirity, Andras Dinnyes, Theerawat Tharasanit, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

Derivation of embryonic stem (ES) cells from parthenogenetic embryos represents a possible alternativeapproach to create histocompatible cells for regenerative medicine. The objectives of this study were to establishmouse parthenogenetic ES (pES) cell lines from parthenogenetically-derived blastocysts as a model system for humanand animal research and to examine pluripotency differences among the pES cell lines. We are able to report thesuccessful establishment of four pluripotent pES cell lines from blastocysts of parthenogenetic origin (22% efficiencyof pES cell line establishment). Four pES cell lines (pES#1-4) exhibited a typical ES cell morphology and expression ofkey pluripotency markers (ALP, Oct4, Nanog and SSEA-1). Three …

Genetic Characterization And Phylogenetic Analysis Of Porcine Circovirus Type 2 In Thai Pigs With Porcine Circovirus Associated Diseases (Pcvad) During 2007-2010, Suphattra Jittimanee, Suparlark Nuntawan Na Ayudhya, Roongtham Kedkovid, Komkrich Teankum, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Porcine circovirus type 2 (PCV2) was first recognized as a causative agent of post weaning multisystemicsyndrome or PMWS in Canada in 1991. Subsequently, it has been reported in almost all intensive pig productioncountries, and currently all associated diseases linked to PCV2 are included under the term porcine circovirusassociated diseases or PCVAD. PCV2 can be divided into 2 major genotypes referred to genotype 1 (PCV2b) andgenotype 2 (PCV2a). In Thailand, PMWS was first reported in 1998. However, only one isolate of Thai PCV2 from ourgroup has been submitted to GenBank (AY864814). Therefore, the objective of this study was to determine thegenetic …

Application Of Flow Cytometry For Early Diagnosis And Monitoring In Cats With Immune-Mediated Hemolytic Anemia, Parkorn Limlenglert, Anudep Rungsipipat, Rosama Pusoonthornthum

The Thai Journal of Veterinary Medicine

The study aimed to apply flow cytometry (FC) for early detection and monitor of treated response cats withIMHA by measuring immunoglobulin-G (IgG) and immunoglobulin-M (IgM) on red blood cells. Coombs' test and FCmethod were performed in twenty-nine cats including: ten clinically normal client-owned cats and nineteen anemiccats with packed cell volume of less than 27%. Five anemic cats were positive for Coombs' test and had significantlyhigh levels of IgG and IgM on the red blood cells with high level of mean fluorescene intensity (MFI) measured by theFC method. In seven out of the fourteen anemic cats with negative Coombs’ results, …

Cell-Cell Interaction Appears To Represent A Pivotal Factor For Susceptibility Of Adult Canine Schwann Cell-Like Brain Glia To Canine Distemper Virus Infection In Vitro, Somporn Techangamsuwan, Enzo Angiolino D'Cundi Orlando, Ilka Imbschweiler, Ingo Gerhauser, Konstantin Wewetzer, Wolfgang Baumgärtner

The Thai Journal of Veterinary Medicine

Recently, we reported the preferential infection of a novel glial cell type from adult canine brain, termedSchwann cell-like brain glia (SCBG) with canine distemper virus (CDV). To further characterize virus-cell and cell-cellinteractions, SCBG in purified and mixed brain cell cultures was infected with different CDV strains. Cell cultureswere infected with the attenuated CDV-Ond, CDV-2544, CDV-OndeGFP, CDV-R252 and the mustelid virulent CDV-5804PeGFP strain. Cytopathic effects (CPEs) and the percentage of infection were monitored daily at 3 and 10 dayspost-infection (dpi), respectively. At 10dpi, the CDV strains induced mild to moderate and severe CPEs in purifiedSCBG and fibroblasts, respectively, while both cell …

Effects Of Erythropoietin On Blood Pressure, Renal Function And Red Blood Cell Production In Dogs With Chronic Kidney Disease With And Without Angiotensin Converting Enzyme Inhibitor, Sujittra Ritthikulprasert, Metinee Rodphol, Adthaphonl Nimrotham, Saikaew Sattayatham, Siripen Komolvanich, Monkon Trisiriroj, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

Blood pressure, renal function, urinary protein excretion and red blood cell count were measured in 29 dogswith chronic kidney disease which received hormone recombinant human Erythropoietin (rHuEPO). The experimentwas divided into 2 parts. Parts 1 comprised 3 groups of dogs. Group 1 dogs were studied prior to erythropoietin(EPO) injection (pre-EPO). Group 2 dogs received EPO 100 U/kg 2-3 times a week. Group 3 dogs received both EPOand angiotensin converting enzyme inhibitor which was enalapril 5 mg/kg/daily. The duration of medication wasbetween 7 to 40 days. In part 2 of the experiment, all parameters were measured in the same dogs before …

Retrospective Histopathological Study Of Hemorrhagic Lesion Of Coumarin Intoxication In Dogs, Theerayuth Kaewamatawong, Acharawan Lohavanijaya, Piyawan Charoenlertkul, Supatra Srichairat

The Thai Journal of Veterinary Medicine

Anticoagulant rodenticide, especially coumarin derivative, is the most common cause of companion animalintoxication. Retrospective pathological examination was performed on 10 dogs suspected to be intoxicated withanticoagulant rodenticides. Coumarin derivatives, cause of intoxication, were confirmed by toxicologicalidentification using thin layer chromatography technique and spectral analysis. Macroscopically and microscopically,all cases showed generalized hemorrhages in various organs. Microscopic examination of hemorrhagic scores of liver,kidneys, intestines, heart, and lungs were 2.28+1.94, 2±2, 1±1.63, 0.8±1.68, and 1.1+2.32, respectively. Highesthemorrhagic score of the liver indicated that it suffered the most hemorrhagic effects related to anticoagulantrodenticides. The important factors might be high blood supply, function of metabolization …

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

Prevalence Of Listeria Monocytogenes In Chicken Production Chain In Thailand, Sasitorn Kanarat, Wongkwan Jitnupong, Jananya Sukhapesna

The Thai Journal of Veterinary Medicine

This study was conducted from 2004 to 2009 to investigate the prevalence of Listeria monocytogenes in thechicken production chain in Thailand from primary production stages to processing plants. A total of 14,670 sampleswere taken from 43 breeder farms, 32 hatcheries, 1331 broiler farms, 22 slaughterhouses and 22 ready-to-eat (RTE)chicken products from processing plants. Various types of samples were collected: soiled litter, water, and chickenfeed from breeder and broiler farms; swabs and mecomium from hatcheries; cloacal swabs and fresh frozen chickenmeat from slaughterhouses; and RTE chicken products from processing plants. This study showed that there was noL. monocytogenes contamination in the …

Seminal Plasma Mda Concentrations Correlating Negatively With Semen Quality In Asian Elephants, Sitthawee Thongtipsiridech, Podjana Imrat, Thanida Srihawong, Sittidet Mahasawangkul, Chanin Tirawattanawanich, Kulnasan Saikhun

The Thai Journal of Veterinary Medicine

The aim of this study was to determine whether lipid peroxidation is correlated with semen quality in Asianelephant bulls. Malondialdehyde (MDA) in seminal plasma from ejaculates with varying percentages of progressivemotility was measured using Thiobarbituric Acid method. Correlation between the MDA levels and percentages ofprogressive motility and normal morphology were performed. Results revealed that the MDA levels weresignificantly negative, which correlated (p<0.05) with the percentages of progressive motility and normal morphology(R=0.2131 and 0.1685, respectively). The results also showed that the MDA levels were significantly differencebetween each bull (p<0.01). Furthermore, when the ejaculates were grouped according to motility scores into twogroups; low- (<40%) and high-percentage of progressive motility (>40%), a significant difference was detectedbetween the MDA means (±SD) of the low- (20.7±11.4 nmol/ml) and high-percentages of progressive motility(14.4±7.8 nmol/ml) groups. The results obtained from this study suggested that MDA could be a potential parameterapplicable for the …

Aquapuncture Using Vitamin B Complex On Intraocular Pressure In Two Dogs With Cataracts And Low Intraocular Pressure, Wei-Yau Shia, Yun-Hsia Hsiao, Ming-Wei Lee, Li-Shiue Shiu, Shiun-Long Lin, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

Two mixed breed dogs, aged 13 years old and 12 years old, with low intraocular pressure are described.These dogs presented with low intraocular pressure (IOP) and cataract. Cataracts were treated for one year by VTPhak®. For the IOP, it was 6 mmHg in both eyes of dog A and 8 mmHg in both eyes of dog B. According to ourevaluation and based on owner’s consent, the dogs should be treated with aquaupuncture using vitamin B complexevery two weeks. Thereafter, the intraocular pressure of these two dogs was evaluated every two months. The localacupoints were BL-1 (Jing Ming), BL-2 (Cuan Zhu), …

Ecg Quiz, Chollada Buranakarl, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Immune Evasion Of Porcine Reproductive And Respiratory Syndrome Virus Through Glycan Shielding Involves Both Glycoprotein 5 As Well As Glycoprotein 3, Hiep Vu, Byungjoon Kwon, Kyoung-Jin Yoon, William W. Laegreid, Asit K. Pattnaik, Fernando A. Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

Passive administration of porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing antibodies (NAbs) can effectively protect pigs against PRRSV infection. However, after PRRSV infection, pigs typically develop a weak and deferred NAb response. One major reason for such a meager NAb response is the phenomenon of glycan shielding involving GP5, a major glycoprotein carrying one major neutralizing epitope. We describe here a type II PRRSV field isolate (PRRSV-01) that is highly susceptible to neutralization and induces an atypically rapid, robust NAb response in vivo. Sequence analysis shows that PRRSV-01 lacks two N-glycosylation sites, normally present in wild-type (wt) PRRSV strains, …

Complete Genome Sequence Of Mycoplasma Suis And Insights Into Its Biology And Adaption To An Erythrocyte Niche, Ana Ms Guimaraes, Andrea P. Santos, Phillip Sanmiguel, Thomas Walter, Jorge Timenetsky, Joanne Messick

Department of Comparative Pathobiology Faculty Publications

Mycoplasma suis, the causative agent of porcine infectious anemia, has never been cultured in vitro and mechanisms by which it causes disease are poorly understood. Thus, the objective herein was to use whole genome sequencing and analysis of M. suis to define pathogenicity mechanisms and biochemical pathways. M. suis was harvested from the blood of an experimentally infected pig. Following DNA extraction and construction of a paired end library, whole-genome sequencing was performed using GS-FLX (454) and Titanium chemistry. Reads on paired-end constructs were assembled using GS De Novo Assembler and gaps closed by primer walking; assembly was validated by …

A Prevalence Study Of Southeast Origin Sale Barn Beef Cattle, Comingled In Warren County, Kentucky, Persistently Infected With Bovine Viral Diarrhea Virus, Including The Effects Of Season And Body Weight, Sarah Elizabeth Thomas

Masters Theses & Specialist Projects

Bovine viral diarrhea (BVD) is an economically important disease of cattle. Calves persistently infected (PI) with the bovine viral diarrhea virus (BVDV) are a powerful agent for spread of the virus. A total of 24,423 southeast origin beef cattle comingled at three Warren County, Kentucky locations were tested from November 2007 to June 2010 for PI BVDV. A total of 97 head tested positive for PI BVDV, giving an average overall prevalence of 0.397%.
Calves tested were subdivided into categories for additional calculations of dependence. A total of 8,910 were categorized by weight range upon testing (300-399 lbs, 400-499 lbs, …

A Mark–Recapture Technique For Monitoring Feral Swine Populations, Matthew M. Reidy, Tyler A. Campbell, David G. Hewitt

United States Department of Agriculture Wildlife Services: Staff Publications

Techniques to monitor populations of feral swine (Sus scrofa) relative to damage control activities are needed on rangelands. Our objectives were to describe and assess a mark–recapture technique using tetracycline hydrochloride (TH) for monitoring feral swine populations. We established bait stations at study sites in southern and central Texas. During 1 d, we replaced normal soured corn bait with bait containing TH and counted the number of feral swine that consumed bait with observers. We conducted feral swine removal using box-style traps and helicopters, at which time we collected teeth for TH analysis. In southern Texas, we estimated …

Molecular Structures Of Prrsv That Contribute To Prrsv Protective Immunity, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

One of the objectives of the proposal is to delineate the regions of the two glycoproteins (GP2 and GP4) of PRRSV that interact with CD163. Other objectives of the proposal are to generate antibodies to these small regions of the glycoproteins as well as to the full-length proteins for future studies to determine if any of these antibodies possess PRRSV neutralizing activity.

To carry out the studies in the proposed objectives, we generated a series of mutants of GP2 as well as GP4 proteins in which various regions were specifically removed by manipulating the plasmids encoding these proteins. We then …

Expression Stability Of Putative Reference Genes In Equine Endometrial, Testicular, And Conceptus Tissues, Claudia Klein, Josep Rutllant, Mats H. T. Troedsson

Veterinary Science Faculty Publications

BACKGROUND: Quantitative RT-PCR data are commonly normalized using a reference gene. A reference gene is a transcript which expression does not differ in the tissue of interest independent of the experimental condition. The objective of this study was to evaluate the stability of mRNA expression levels of putative reference genes in three different types of equine tissue, endometrial, testicular, and conceptus tissue.

FINDINGS: The expression stability of four (uterine tissue) and six (testicular and conceptus tissue) was assessed using descriptive data analysis and the software programs Normfinder and geNorm. In uterine samples, 18S showed the largest degree of variation in …

Growing South Dakota (Spring 2011), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Classroom to Careers: An Optimistic Outlook Awaits Ag & Biological Sciences Graduates
[Page] 5 New Opportunities: Intro Ag Courses To Be Offered in Rapid City
[Page] 6 Budget Reality: Funding Cuts Prompt Structure and Staff Changes
[Page] 7 Gearing Up for DakotaFest: Replicated SDSU Campus, Educational Forums and Fundraising Auction Planned
[Page] 8 Continuing a Legacy: &1 Million Livestock Judging Endowment Being Established
[Page] 10 4-H Fun: Unique 4-H Activities Spark Student Interest in Science
[Page] 12 Innovative Outreach: BBQ Boot Camp Connect Consumers to Meat Industry
[Page] 13 College News
[Page] 14 Relevant Research: New, More Efficient …