Effects Of Queen Rearing Period On Reproductive Features Of Italian (Apis Mellifera Ligustica), Caucasian (Apis Mellifera Caucasica), And Aegean Ecotype Of Anatolian Honey Bee (Apis Mellifera Anatoliaca) Queens, Aytül Uçak Koç, Mete Karacaoğlu
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the effects of rearing period on the quality characteristics of honey bee queens reared from Italian and Caucasian races and from the Aegean ecotype of Anatolian honey bee under the conditions of the Aegean region. Italian (I), Caucasian (C), and Aegean (A) queens were reared in April, June, and August of 2007 and 2008. The queens were weighed on the second day after emergence (WQ) and at the onset of oviposition (OWQ). The interval from emergence to the onset of oviposition (preoviposition period, POP) was determined in 3 genotype queen groups. The numbers of …
Improvement Studies On Mutton Sheep For Marmara Region Conditions: I. Fertility, Lamb Survival, And Growth Traits Of Lambs,
2011
TÜBİTAK
Improvement Studies On Mutton Sheep For Marmara Region Conditions: I. Fertility, Lamb Survival, And Growth Traits Of Lambs, Ayhan Ceyhan, Tamer Sezenler, İsmai̇l Erdoğan, Osman Torun
Turkish Journal of Veterinary & Animal Sciences
The present study was conducted to compare the fertility of ewes, the survival rates, and the growth performance of lambs that were crossbred by mating German Black-Headed Mutton (GBM) rams with Kıvırcık ewes. The average lambing rates of the GBM × K(F1), Bandırma-I and Bandırma-II genotype, and Kıvırcık ewes were 80.00%, 75.73%, 76.78%, and 83.72%, respectively. The differences in lambing rates between the genotypes were significant (P < 0.01). The genotypes had a significant effect on litter size at birth, but not on fecundity. The differences in lambs' survival rates between the genotypes were significant (P < 0.05). The birth weight (BW), weaning weight (WW), and yearling live weight (YLW) of the lambs were 3.77 kg, 34.11 kg, and 43.71 kg for the GBM × K (F1); 3.74 kg, 32.98 kg, and 44.91 kg for the Bandırma-I; 3.73 kg, 33.18 kg, and 45.71 kg for the Bandırma-II; and 3.45 kg, 30.92 kg, and 42.22 kg for the Kıvırcık lambs. The results of this study showed that crossbreeding between the GBM and Kıvırcık breed did not significantly increase lamb production. However, crossbred lambs did not have significant survival problems when compared to native Kıvırcık lambs and had higher growth performance than purebred Kıvırcık lambs.
Inhibitory Activity And Adhesive Ability Of Potential Probiotic Bacillus Species To Confer Protection For Artemia Gnotobiotic Culture Against Pathogenic Vibrio Spp., Abdelkarim Mahdhi, Fathi Kamoun, Amina Bakhrouf
Turkish Journal of Veterinary & Animal Sciences
To evaluate the potential probiotic effect of B. coagulans and B. subtilis strains toward Artemia cultured in different gnotobiotic conditions, antibacterial and adherence assays were investigated and challenge tests with Artemia larvae were performed. Virulent V. alginolyticus (IFL), isolated from infected fish, was tested for comparative purposes. The bacterial strains B. coagulans and B. subtilis produced antimicrobial activity against the pathogenic Vibrio species, including V. alginolyticus isolated from infected fish, and inhibitory zones that ranged from 12 to 20 mm in diameter. An adherence assay revealed that these potential probiotic bacteria are fairly adherent, with a values ranging from 0.10 …
A Molecular Study Of The Salmonella Enterica Serovars Abortusovis, Typhimurium, And Enteritidis,
2011
TÜBİTAK
A Molecular Study Of The Salmonella Enterica Serovars Abortusovis, Typhimurium, And Enteritidis, Taghi Zahraei Salehi, Omid Madadgar, Hasan Tadjbakhsh, Mohammad Reza Mahzounieh, Mohammad Mehdi Feizabadi
Turkish Journal of Veterinary & Animal Sciences
This study presents a molecular analysis of 3 important human and animal serovars of Salmonella: Typhimurium, Enteritidis, and Abortusovis. We also provide information that can be applied in the surveillance of salmonellosis. Over the course of 3 decades, 90 isolates were collected, with 30 isolates representing each type of serovar. In addition to conventional serotyping, serovars were confirmed by polymerase chain reaction (multiplex PCR and IS200 PCR). Pulsed-field gel electrophoresis (PFGE) of the serovars confirmed 12, 15, and 20 patterns, respectively. In an analysis of the results, 5 pulsotypes (80% of isolates) of Salmonella Typhimurium and 4 pulsotypes (64% of …
Evaluation Of Mass Spectrometric Methods For Detection Of The Anti-Protozoal Drug Imidocarb,
2011
Michigan State University
Evaluation Of Mass Spectrometric Methods For Detection Of The Anti-Protozoal Drug Imidocarb, A. F. Lehner, J. A. Hitron, J. May, C. Hughes, R. Eisenberg, N. Schwint, D. P. Knowles, P. Timoney, Thomas Tobin
Maxwell H. Gluck Equine Research Center Faculty Publications
Imidocarb [N,N'-bis[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]urea, C19H20N6O1, m.w. 348.41] is a symmetrical carbanilide derivative used to treat disease caused by protozoans of the Babesia genus. Imidocarb, however, is also considered capable of suppressing Babesia-specific immune responses, allowing Babesia-positive horses to pass a complement fixation test (CFT) without eliminating the infection. This scenario could enable Babesia-infected horses to pass CFT-based importation tests. It is imperative to unequivocally identify and quantify equine tissue residues of imidocarb by mass spectrometry to address this issue. As a pretext to development of sensitive tissue assays, we have investigated possibilities of mass …
The Impact Of Tricaine Methanesulfonate, 2-Phenoxyethanol, And Carvone-Methyl Salicylate On The Innate Immune Response Of Zebrafish (Danio Rerio),
2011
Colby College
The Impact Of Tricaine Methanesulfonate, 2-Phenoxyethanol, And Carvone-Methyl Salicylate On The Innate Immune Response Of Zebrafish (Danio Rerio), Charles R. Wulff
Honors Theses
Anesthesia plays a vital role in the maintenance of aquaculture species, where it is used to minimize stress during complex handling tasks such as transport, assessment, and harvesting. However, anesthetics have been shown to suppress the innate immune response, which could impact immunity and increase risk of infection. Tricaine methanesulfonate (MS-222) and 2-Phenoxyethanol (2-PE) represent two of the most commonly used anesthetics in aquaculture, with R-(+)-carvone, in the form of carvone-methyl salicylate (CMS) has recently been proposed as an alternative anesthetic for food fish. These three anesthetics were used to assess the influence of anesthetics on the immune system of …
Double-Layer Mesh Hernioplasty For Repairing Umbilical Hernias In 10 Goats*,
2011
TÜBİTAK
Double-Layer Mesh Hernioplasty For Repairing Umbilical Hernias In 10 Goats*, Jose M. Vilar, Juan A. Corbera, Giuseppe Spinella
Turkish Journal of Veterinary & Animal Sciences
Umbilical hernias in goats are uncommon and can vary in their etiology and management. Hernioplasty can be done by closing the abdominal wall with a horizontal mattress pattern using absorbable sutures. However, larger defects (hernial ring size >3 cm) generally require the use of prosthetic materials that allow for a tension-free repair. In this study, 10 young female goats with umbilical hernias and hernial ring sizes ranging from 7-10 cm in width were treated using a double-layer polypropylene mesh. An ultrasonographic examination was performed to assess healing and connective tissue production.
Genetic Immunization In The Horse: The Potential For Enhanced Immune Responses With Deacylated Polyethyleneimine (Pei) And Immunostimulatory Cytokines As Vaccine Adjuvants,
2011
University of Kentucky
Genetic Immunization In The Horse: The Potential For Enhanced Immune Responses With Deacylated Polyethyleneimine (Pei) And Immunostimulatory Cytokines As Vaccine Adjuvants, Deborah Lee Even
Theses and Dissertations--Veterinary Science
DNA vaccines in larger animals, such as horses, are generally less effective and elicit significantly weaker immune responses, than in small animal model systems. To provide optimal protection against pathogenic microorganisms, the induction of both humoral and cellular immune responses from DNA vaccination may be necessary. One limitation to DNA immunization in the horse is the difficulty in generating high levels of antigen-specific antibody and CTL responses. Previous work in the laboratory has demonstrated that expression constructs containing native sequences encoding the surface unit (SU) envelope glycoprotein (pCiSU) of the Equine Infectious Anemia Virus (EIAV) are ineffective at stimulating immune …
Arbovirus Phenotype Alters Transmission Potential,
2011
Louisiana State University and Agricultural and Mechanical College
Arbovirus Phenotype Alters Transmission Potential, Rebecca C. Christofferson
LSU Doctoral Dissertations
Extrinsic and environmental factors are known to affect the transmission of arthropod-borne viruses (arboviruses), including variations in the arthropod vector populations. Differences among these factors have been associated with differential transmission and are sometimes used to control the spread of an arbovirus through a vertebrate population in an effort to prevent or disrupt an outbreak. However, diversity in intrinsic viral populations, such as genetic and phenotypic variability, is not often accounted for when considering alterations in transmission. Presented in this dissertation are four experimental studies that explore the contribution of viral intrinsic factors, especially phenotypic variability, to the transmission potential …
Biomechanical Evaluation Of Medial And Lateral Approaches For Experimentally Created Medial Condylar Fractures Of The Equine Third Metacarpal Bone,
2011
Louisiana State University and Agricultural and Mechanical College
Biomechanical Evaluation Of Medial And Lateral Approaches For Experimentally Created Medial Condylar Fractures Of The Equine Third Metacarpal Bone, Saybl Beauton Sprinkle
LSU Master's Theses
Objective -To compare the compression produced in reduction of experimentally created medial condylar fractures using lag screw fixation with medial vs. lateral approach, and to determine the maximum torque at screw failure. Materials and Methods- Twelve (12) pairs (left and right) 3rd metacarpal bones (MC3) were collected from adult (2-7 years) Thoroughbreds euthanized for reasons unrelated to orthopedic disease. Complete parasagittal medial condylar osteotomies were created at a measurement of 9, 13, and 21 mm axial to the epicondylar fossa on four pairs each of cadaveric MC3 bones resulting in fracture fragments measuring 8, 12, and 20 mm in thickness. …
Duration Of Immunity Following The Administration Of Oil-Based Avian Influenza H_5n_1 Vaccine In Layers,
2011
TÜBİTAK
Duration Of Immunity Following The Administration Of Oil-Based Avian Influenza H_5n_1 Vaccine In Layers, Maryam Nisar, Anwar-Ul Haq, Asif Rashid, Muhammad Iqbal
Turkish Journal of Veterinary & Animal Sciences
Avian influenza (AI) occurs worldwide and causes tremendous economic losses. The disease is characterised by respiratory signs, depression, and reduced food and water intake. In the present study, an oil-based vaccine created by using Montanide ISA 70 MVG, was prepared and the duration of immunity checked at different time intervals. For this purpose, the cumulative mean titre (CMT) was calculated after employing haemagglutination inhibition test in 50 pullets at day zero before vaccination and then inoculated 0.3 mL of emulsified vaccine subcutaneously in 40 birds while 10 birds were kept non-vaccinated as a control group throughout the experiment. On day …
Aflatoxin M1 Contamination In Cow’S Milk In Kayseri (Central Turkey),
2011
TÜBİTAK
Aflatoxin M1 Contamination In Cow’S Milk In Kayseri (Central Turkey), Hi̇kmet Mavuş Buldu, A. Nedret Koç, Güven Uraz
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to determine the levels of aflatoxin M1 (AFM1) in the milk of cows in the villages of Kayseri province, Turkey. Ninety milk samples were investigated for AFM1 contamination using the enzyme linked immunoassay (ELISA). The mean value of AFM1 levels was 59.9 ng/L, 95% confidence interval was 54.4-65.5 ng/L, and standard deviation was 26.6 ng/L. For 63 samples (70%) the values were significantly above the limit permitted by European Union criteria (EU) and the Turkish Food Codex (TFC) (P < 0.05). However, the differences in AFM1 levels between villages were not significant (P > 0.05). In conclusion, since the level of AFM1 in the milk of cows was higher …
Eosinophils In Lung Tissue In 2 Chilean Criollo Horses With Exacerbated Recurrent Airway Obstructions “Heaves”,
2011
TÜBİTAK
Eosinophils In Lung Tissue In 2 Chilean Criollo Horses With Exacerbated Recurrent Airway Obstructions “Heaves”, Alexander Ortloff, Hugo Folch, Gabriel Moran
Turkish Journal of Veterinary & Animal Sciences
We report on 2 cases of recurrent airway obstruction (RAO) observed in Chilean Criollo horses aged 13 and 15 years. Both animals were classified as having severe RAO. The analysis of the recovered cells of bronchoalveolar lavage fluid (BALF) showed high percentages of eosinophils (82% and 80% in the differential cell counts of the 2 horses) and poor responses to bronchodilator and corticosteroid drugs. The histopathological lung evaluation showed eosinophilic infiltration in the peribronchial tissue, as well as in the lumen of the bronchi and bronchioli. In both horses, immunohistochemistry confirmed high p65 homodimer activity in bronchial cells. This report …
Disodium Edta Used As Anticoagulant Causes Hemolysis In Common Carp Blood,
2011
TÜBİTAK
Disodium Edta Used As Anticoagulant Causes Hemolysis In Common Carp Blood, Malgorzata Witeska, Wioleta Wargocka
Turkish Journal of Veterinary & Animal Sciences
Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples. Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis). The nuclei released from destroyed cells changed shape from oval to round, and underwent gradual swelling and vacuolation, followed by karyolysis. The degree of observed changes was similar at all Na2EDTA concentrations (0.1, 0.5, and 1.0 mg/mL); on the other hand, percentage of abnormal and destroyed cells showed considerable differences among blood sampled from various carp individuals. The obtained results revealed that Na2EDTA caused gradual damage to …
Bilateral Testicular Hypoplasia Among Mature Sahel Bucks In Nigeria,
2011
TÜBİTAK
Bilateral Testicular Hypoplasia Among Mature Sahel Bucks In Nigeria, Ikechukwu Igbokwe, Augustine Ikpo, Hafsat Grema, Fakilahyel Mshelbwala, Nanacha Igbokwe
Turkish Journal of Veterinary & Animal Sciences
Among 1608 Sahel goat bucks presented for slaughter at the Maiduguri Metropolitan abattoir in March-April 2007, 65.7% had plain white or black-white coat colour, and 91.2% were aged >1.5-3.0 years with mean body weights of 15.6-25.7 kg. Twenty-two (1.4%) of the mature bucks, aged 1.9 ± 0.3 years and weighing 20.6 ± 3.5 kg, had bilateral small hypoplastic testes that were symmetric, approximately 7 times smaller in weight, with a 2-fold reduction in longitudinal length and mid-circumference than the full-sized normal testes. The epididymes were also reduced in size as the testes. Histologically, the seminiferous tubules and caudal epididymides showed …
Statistical Evaluation Of Some Environmental Factors Affecting The Gross Efficiency Of Crude Protein In High Producing Dairy,
2011
TÜBİTAK
Statistical Evaluation Of Some Environmental Factors Affecting The Gross Efficiency Of Crude Protein In High Producing Dairy, Pouya Zamani, Seyed Reza Miraei Ashtiani, Daryoush Alipour, Hasan Aliarabi, Ali Asghar Saki, Ahmad Ahmadi, Khalil Zaboli
Turkish Journal of Veterinary & Animal Sciences
Some environmental factors affecting the gross efficiency of crude protein (GECP) were studied using 2824 records of 501 lactating Holstein cows. The effects of some independent variables on the GECP were tested using a general linear model analysis. The GECP was significantly affected by parity and pregnancy stage and linearly related to dry matter intake, milk yield, milk fat, protein and lactose percentages, protein and lactose yields, dietary levels of net energy for lactation (NEL), crude protein (CP), ruminally undegradable protein (RUP), and ether extract (EE). Quadratic relation to the GECP with dietary levels of NEL, CP, RUP, and EE …
Analysis Of Buffalo Liver Samples For The Presence Of Chlorpyrifos Residues By Using High Performance Liquid Chromatography,
2011
TÜBİTAK
Analysis Of Buffalo Liver Samples For The Presence Of Chlorpyrifos Residues By Using High Performance Liquid Chromatography, Pradeep Kumar, S.P. Singh, K. Shrikant, D. Madhukar
Turkish Journal of Veterinary & Animal Sciences
Food safety is of great importance to all involved in the food chain. The problem of pesticide residues is one of the concerns for the consumers. In the present study, 254 buffalo liver tissue samples were collected from 4 slaughterhouses and analyzed both qualitatively and quantitatively for the presence of chlorpyrifos residues. The samples were processed by extraction with acetonitrile followed by sonication and liquid-liquid partition. The extracts were cleaned up by performing alumina column chromatography. Chlorpyrifos residues in the samples were detected and quantified by employing high performance liquid chromatography. High performance liquid chromatography (HPLC) was performed by using …
Expression And Detection Of The Fmdv Vp1 Transgene And Expressed Structural Protein In Arabidopsis Thaliana,
2011
TÜBİTAK
Expression And Detection Of The Fmdv Vp1 Transgene And Expressed Structural Protein In Arabidopsis Thaliana, Li Pan, Yongguang Zhang, Yonglu Wang, Jianliang Lv, Peng Zhou, Zhongwang Zhang, Yuzhen Fang, Shoutian Jiang
Turkish Journal of Veterinary & Animal Sciences
To explore the feasibility of developing a new type of plantderived foot-and-mouth disease virus (FMDV) oral vaccine, the plant seed-specific expression vector p7SBin438/VP1 carrying the VP1 gene of the FMDV strain O/China/99 was constructed and transformed into Agrobacterium tumefaciens strain GV3101. This strain was used for transformation of Arabidopsis thaliana via the floral-dip method. The kanamycin-resistant transgenic plants were selected, and the VP1 gene and protein expressions were confirmed by PCR and sandwich-ELISA, respectively. The ELISA positive plants were further confirmed by Western blot assay, and the VP1 gene in the transgenic progeny was confirmed by PCR. The results showed …
Pathological And Immunohistochemical Evaluation Of The Effects Of Interferon Gamma (Ifn-Γ) And Aminoguanidine In Rats Experimentally Infected With Fasciola Hepatica, Enver Beytut, Ati̇la Akça, Hali̇l İbrahi̇m Gökce
Turkish Journal of Veterinary & Animal Sciences
The present study was aimed at pathologically and immunohistochemically evaluating the effects of interferon gamma (IFN-\gamma) and aminoguanidine (AG) in rats experimentally infected with Fasciola hepatica. A total of 44 Wistar rats were divided into 4 groups. With the exception of the controls (fourth group), the remaining rats were infected orally with 25 metacerceria of F. hepatica. The first group was administered 0.2% AG in drinking water daily, in order to block nitric oxide (NO) production. The second group was administered 250 units of IFN-\gamma daily, in order to stimulate NO synthesis. The third group was administered a placebo only. …
Economic Selection Index Using Different Milk Production Traits Of Holstein And Its Crossbreds,
2011
TÜBİTAK
Economic Selection Index Using Different Milk Production Traits Of Holstein And Its Crossbreds, Md. Kabirul Islam Khan, Jewel Mazumder
Turkish Journal of Veterinary & Animal Sciences
The study was conducted to estimate the Best Linear Unbiased Prediction (BLUP) breeding values of different milk production traits for Holstein and its crossbreds and to construct an economic selection index or total merit for individual cows from 2 different dairy farms in Chittagong, Bangladesh. The BLUP-estimated breeding values were obtained by AIREML, and an economic selection index was constructed as the sum of the product of the estimated breeding value with the economic value for each trait (lactation milk yield, calving interval, and liveweight). The Holstein × Sahiwal crossbred showed better phenotypic performance in both farms while the same …
