Antagonistic Effects Of Cellular Poly(C) Binding Proteins
On Vesicular Stomatitis Virus Gene Expression,
2011
University of Nebraska-Lincoln
Antagonistic Effects Of Cellular Poly(C) Binding Proteins On Vesicular Stomatitis Virus Gene Expression, Phat X. Dinh, Lalit Beura, Debasis Panda, Anshuman Das, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
Immunoprecipitation and subsequent mass spectrometry analysis of the cellular proteins from cells expressing the vesicular stomatitis virus (VSV) P protein identified the poly(C) binding protein 2 (PCBP2) as one of the P protein-interacting proteins. To investigate the role of PCBP2 in the viral life cycle, we examined the effects of depletion or overexpression of this protein on VSV growth. Small interfering RNA-mediated silencing of PCBP2 promoted VSV replication. Conversely, overexpression of PCBP2 in transfected cells suppressed VSV growth. Further studies revealed that PCBP2 negatively regulates overall viral mRNA accumulation and subsequent genome replication. Coimmunoprecipitation and immunofluorescence microscopic studies showed that …
Observation Of Ruminococcus Strains In Captive Asian Elephant (Elephas Maximus),
2011
Chulalongkorn University
Observation Of Ruminococcus Strains In Captive Asian Elephant (Elephas Maximus), Nattawooti Sthitmatee, Pranisa Mahatnirunkul, Patcharaporn Keawmong, Anucha Sirimalaisuwan, Pinich Boontong, Suvichai Rojanasthien, Sumalee Boonmar
The Thai Journal of Veterinary Medicine
Asian elephant is indigenous to many countries including Thailand, but fermenter microorganisms ingastrointestinal tract of the elephant have not fully been investigated. Therefore, this study aimed to observe thecellulolytic bacteria in Genus Ruminococcus in large intestines of captive Asian elephants (Elephas maximus). Fecalsamples were collected from male and female sucklings, young and adult captive Asian elephants. Forty-fourelephants were divided into 3 groups as followed: A) > 18 years old (n=24); B) 2-18 years old (n=17) and C) < 2 yearsold (n=3). The results revealed that there were 214 (42.8%) isolates of R. flavefaciens, 105 (21.0%) isolates of R. bromii, 90(18.0%) isolates of R. obeum, 54 (10.8%) isolates of R. callidus and 37 (7.4%) isolates of R. albus from all fecal samplesexamined. Interestingly, Ruminococcus strains could be isolated from the weaned elephants, but were not found in thesucklings (p<0.05). In conclusion, cellulolytic bacteria in Genus Ruminococcus were isolated from the large intestines ofcaptive Asian elephants. Moreover, the highest prevalence of the bacteria was found in the elephants aged more than18 years old.
Differences Of Periods Of Calving On Days Open Of Dairy Cows In Different Regions And Months Of Thailand,
2011
Chulalongkorn University
Differences Of Periods Of Calving On Days Open Of Dairy Cows In Different Regions And Months Of Thailand, Winai Kaewlamun, Rut Chayaratanasin, Prachin Virakul, Andrew A. Ponter, Patrice Humblot, Siriwat Suadsong, Padet Tummaruk, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The objectives of this study were to analyze the potential impact of heat stress on reproduction in differentregions of Thailand, to determine the monthly distribution of calving throughout the year and to investigateenvironmental sources of variation of days-open (DO) in first lactation dairy cows. Data including 13,548 lactationrecords were collected during 2004 to 2006. Climate data were obtained from the provincial meteorological stationsand the corresponding temperature-humidity indexes were calculated. The difference between regions in THI wasdetermined. The geographical regions studied were: Central, Eastern, Northeastern, and Northern. The distributionof calving by month was determined in the 1st to 5th lactation. The …
Effect Of Equex Stm Paste On The Quality And Motility Characteristics Of Post Thawed Cryopreserved Goat Semen,
2011
Chulalongkorn University
Effect Of Equex Stm Paste On The Quality And Motility Characteristics Of Post Thawed Cryopreserved Goat Semen, Nitira Anakkul, Junpen Suwimonteerabutr, Jinda Singlor, Nawapen Phutikanit, Theerawat Tharasanit, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The aim of this study was to evaluate the effect of Equex STM Paste on goat semen quality aftercryopreservation. Semen samples were consecutively collected once a week, from six mature bucks. The semensamples with adequate motility (>60%) and sperm concentration (>1,500x106 sperm/ml) were frozen in an egg yolk-Tris semen extender supplemented either with (E+) or without (E-) Equex STM Paste. The semen quality includingmotility, viability, morphology, acrosome integrity and membrane functional integrity were evaluated beforeprocessing and after cryopreservation. Computer-assisted-semen analysis (CASA) was used to characterize the spermmotion patterns during the thermal resistance test at 0, 1, 2 and …
Effects Of Dietary Protein Deficiency On Plasma Amino Acids And Physical – Histological Conformation Of The Mammary Glands In Lactating Sows,
2011
Chulalongkorn University
Effects Of Dietary Protein Deficiency On Plasma Amino Acids And Physical – Histological Conformation Of The Mammary Glands In Lactating Sows, Patrapan Rungcharoen, Sarinee Kalandakanond-Thongsong, Boonrit Thongsong
The Thai Journal of Veterinary Medicine
Eleven multiparous crossbred lactating sows were used to determine the influence of deficient protein dietcontaining 8.2% crude protein and normal protein diet containing 18.2% crude protein on plasma amino acidconcentrations of sows and their piglets, physical mammary gland and histological changes of mammary cells. Theywere provided sufficient water and feed access to one of two isocaloric diets, either deficient (6 sows) or normalprotein diets (5 sows). The experimental period started from farrowing (day 0) until weaning day (day 28). In thisstudy, sows fed deficient protein diet had significant increase in body weight losses and their piglets had significantdecrease in body …
Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo,
2011
Chulalongkorn University
Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan
The Thai Journal of Veterinary Medicine
Virus infection requires the interaction of a virus protein with the host-cell receptor. The Influenza A virusreceptor consists of sialic acid linked to the galactose unit in a α 2,3 or α 2,6 conformation. Various types of thesereceptors are expressed differently on the epithelial lining of various animal species. Here we characterized the typesof receptors that are expressed in the upper and lower respiratory tracts of cow, buffalo, pig and quail. Our findingsdemonstrate that SA α 2,6-gal linked receptors for human influenza viruses are present in the lower respiratory tractof cow and buffalo, while the SA α 2,3-gal linked receptors …
Detection Of West Nile Virus In Mosquitoes In Nakhon-Pathom And Phetchaburi Province, Thailand,
2011
Chulalongkorn University
Detection Of West Nile Virus In Mosquitoes In Nakhon-Pathom And Phetchaburi Province, Thailand, Kriangkrai Witoonsatian, Nuananong Sinwat, Rungroj Jam-On, Khongsak Thiangtum, Thaweesak Songserm
The Thai Journal of Veterinary Medicine
West Nile (WN) disease is one of the encephalitic diseases in humans and mammals. This disease has beenreported in several areas of the world. Thailand is located in the area of high risk of WN virus (WNv) infectionconcerning a natural transmission cycle. However, the WNv infection has not been previously reported. Therefore,the aim of this study was to detect WNv in mosquitoes in some areas in Nakhon-pathom and Phetchaburi provinces,central Thailand. The period of this study was done during November 2008 to November 2010. We found that therewere four genera collected including Aedes, Anopheles, Culex and Mansonia. Culex spp such …
What Is Your Diagnosis,
2011
Chulalongkorn University
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep
The Thai Journal of Veterinary Medicine
No abstract provided.
World Veterinary Year 2011 250 Years Veterinary Education In The World,
2011
Chulalongkorn University
World Veterinary Year 2011 250 Years Veterinary Education In The World, Achariya Sailasuta
The Thai Journal of Veterinary Medicine
No abstract provided.
Evaluation Of Microsatellite Polymorphism And Genetic Variability In Thai Ridgeback And Bangkaew Dogs,
2011
Chulalongkorn University
Evaluation Of Microsatellite Polymorphism And Genetic Variability In Thai Ridgeback And Bangkaew Dogs, Janjira Phavaphutanon, Sudtisa Laopiem
The Thai Journal of Veterinary Medicine
The origin of dogs came from the limited number of founders leading to inbreeding or genetic diseasewithin or among breeds. Thai Ridgeback (TR) and Bangkaew (BK) dogs were the only native dog breeds that webelieved to originate in Thailand. The study of genetic variability in Thai dogs (39 Thai Ridgeback dogs, 82 Bangkaewdogs) was defined by using 12 microsatellite markers. These DNA markers were highly informative and distributedthrough the canine genome. The result from these markers revealed the higher number of alleles, observedheterozygosity (Ho), expected heterozygosity (He) and polymorphic information content (PIC) in TR and BK dogs.The average Ho, He …
The Effect Of Using Long Term And Short Term Extenders During Cooling Process On The Quality Of Frozen Boar Semen,
2011
Chulalongkorn University
The Effect Of Using Long Term And Short Term Extenders During Cooling Process On The Quality Of Frozen Boar Semen, Kampon Kaeoket, Primsiri Chanapai, Puriwat Junchiyaphoom, Panida Chanapiwat
The Thai Journal of Veterinary Medicine
Cooling process of cryopreservation is one of the important factors that affect the qualities of frozen-thawedboar semen. The objective of this study was to compare the qualities of frozen-thawed boar semen after usingdifferent extenders (i.e. BTS, Vitasem LD, ModenaTM and Androstar® plus) during cooling process. Eight sperm-richfractions from 8 boars were employed. The ejaculated semen sample from each boar was divided into four groupsand extended in different freezing extender I as follows: group I (control, short term, BTS), group II (long term,Vitasem LD), group III (long term, ModenaTM) and group IV (long term, Androstar® plus) and kept at 15oC for …
Investigation Of The Relationship Between Endometrial Mmp Activities And Sex Hormones In Mature Bitches,
2011
Chulalongkorn University
Investigation Of The Relationship Between Endometrial Mmp Activities And Sex Hormones In Mature Bitches, Wei-Yau Shia, Po-Chen Chu, Chih-Chung Chou, Wei-Ming Lee
The Thai Journal of Veterinary Medicine
Endometrial matrix metalloproteinases (MMPs) are extensively studied in humans. However, owing to thedistinct differences of the estrous cycle of bitches, the expression and activities of endometrial MMPs regulated by sexhormones should be significantly different. To understand the effect of sex hormones on the activity of MMPs, thisstudy investigates the activities of endometrial MMP-2 and MMP-9 during different phases of the estrous cycle inbitches through the use of gelatine zymography. Estradiol and progesterone concentrations were also determinedusing enzyme-linked fluorescent assay. The activities of the latent forms of both MMP-2 and -9 were significantlyhigher in metestrus (MP) and late diestrus phases (L-DP) …
Analysis On Differentially Expressed Genes Of Muscle Tissues In Rugao Chicken At The Ages Of 2 And 12 Weeks By Microarray,
2011
Chulalongkorn University
Analysis On Differentially Expressed Genes Of Muscle Tissues In Rugao Chicken At The Ages Of 2 And 12 Weeks By Microarray, Qin De Luan, Guo Bin Chang, Ting Zhen, Zheng Yang Huang, Wei Zhou, Yan Liu, Guo Hong Chen
The Thai Journal of Veterinary Medicine
Microarray containing 13319 probes was used to construct gene expression profiles in order to screendifferentially expressed genes of muscle tissues in Rugao chicken and investigate the molecular mechanism relatedwith muscle tissue traits at 2 and 12 weeks old of chicken. Two hundred and eight differentially expressed genes, ofwhich 108 were known genes, 94 up-regulated, 114 down-regulated were involved in growth, molecular mechanism,fat metabolism, cell proliferation, transcription and splicing factor, protein synthesis and degradation. Meanwhile,some genes of these differentially expressed genes that had no annotation in GenBank were screened out, they werepresumed to be unknown new genes. The differential expressions of …
Effect Of Water Temperature On Susceptibility To Streptococcus Agalactiae Serotype Ia Infection In Nile Tilapia (Oreochromis Niloticus),
2011
Chulalongkorn University
Effect Of Water Temperature On Susceptibility To Streptococcus Agalactiae Serotype Ia Infection In Nile Tilapia (Oreochromis Niloticus), Channarong Rodkhum, Pattanapon Kayansamruaj, Nopadon Pirarat
The Thai Journal of Veterinary Medicine
Streptococcus agalactiae is one of the causative agents associated with warm-water streptococcosis, in whichproduce massive mortality in aquaculture. Emergence of disease in tilapia farm usually occurs in high temperatureseason, which suggested for higher susceptibility of tilapia in this particular condition. Thus, the objective of thisstudy is to investigate the association between water temperature and susceptibility of Nile tilapia (Oreochromisniloticus) to S. agalactiae serotype Ia infection. Nile tilapia were inoculated with 108, 107 or 106 CFU/ml of S. agalactiaeserotype Ia field strain via water immersion route and maintained in different water temperature at 25, 30 or 33oC for1 week. Diseased fish …
Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan,
2011
Chulalongkorn University
Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura
The Thai Journal of Veterinary Medicine
We studied the prevalence of swine salmonellosis and PFGE genotype of isolates in Gunma Prefecture,Japan. Between 2005 and 2008, swine salmonellosis was confirmed in 430 of 2,707,402 (0.02%) swine atslaughterhouses. All isolates were identified as deriving from Salmonella Choleraesuis, biotype Choleraesuis (negativefor H2S production). We used 30 bacterial strains from 15 farms that had experienced outbreaks in 2006 and 2007. Allstrains were susceptible to various antibiotics such as cephems (cefotaxime), fluoroquinolones (norfloxacin andciprofloxacin), and fosfomycin. On the other hand, all strains were resistant to tetracycline (TC), and 29 of 30 (97%)strains were resistant to streptomycin (SM). The most predominant profiles …
Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct,
2011
Chulalongkorn University
Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The glycosaminoglycans (GAGs) found in the mammalian oviductal tissue and luminal secretory fluidinfluence the fertilization processes in various patterns such as sperm storage, viability and capacitation. The aim ofthis study was to confirm the appearance of hyaluronan and syndecan-1 in the swamp buffalo oviduct consisting ofuterotubal junction (UTJ), isthmus, ampulla and infundibulum during oestrous cycle using histochemical andimmunohistochemical techniques. The non-sulphated GAGs, i.e. hyaluronan (HA), were noticeably localized in thesubepithelial connective tissue layer or lamina propria of all segments while the epithelial HA-positive staining wasdemonstrated only in the UTJ and isthmus (sperm reservoir) at the follicular phase. In contrast, the …
Effect Of Roselle (Hibiscus Sabdariffa Linn.) Calyx In Laying Hen Diet On Egg Production Performance, Egg Quality And Tbars Value In Plasma And Yolk,
2011
Chulalongkorn University
Effect Of Roselle (Hibiscus Sabdariffa Linn.) Calyx In Laying Hen Diet On Egg Production Performance, Egg Quality And Tbars Value In Plasma And Yolk, Piyaphon Sukkhavanit, Kris Angkanaporn, Suwanna Kijparkorn
The Thai Journal of Veterinary Medicine
An experiment was conducted to investigate the effect of Roselle calyx in two preparation forms in layerdiets on egg production performance, egg quality and TBARS value in plasma and yolk. Two hundred and seventy,33-week-old, CP Brown laying hens were randomly allocated into 6 treatments with 5 replications of 9 hens each.The dietary treatments were control diet, control diet supplemented with 250 mg/kg α-tocopheryl acetate, dietcontaining 1 and 2% of Roselle calyx crude extracts, and diet containing 2% and 4% Roselle calyx powder. Eggproduction performance was recorded, egg quality was measured and TBARS value in plasma and yolk wereanalyzed after fed …
Seroprevalence And Risk Factors Associated With Caprine Arthritis-Encephalitis Virus Infection In Goats In The Western Part Of Thailand,
2011
Chulalongkorn University
Seroprevalence And Risk Factors Associated With Caprine Arthritis-Encephalitis Virus Infection In Goats In The Western Part Of Thailand, Thant Nyi Lin, Saroch Ngarmkum, Kanisak Oraveerakul, Prachin Virakul, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
To determine the seroprevalence of caprine arthritis-encephalitis virus infection (CAEV) in goats in theWestern part of Thailand, a cross-sectional serological survey was conducted in three provinces, Ratchaburi,Petchaburi and Kanchanaburi, situated in the western part of the country along the border area. A total of 1,129serum samples from 74 randomly selected goat farms containing different breeds of goats were collected during theperiod from November 2009 to January 2011. Seroprevalence of CAEV antibodies was determined using competitiveenzyme-linked immunosorbent assay (cELISA) test. To investigate the risk factors associated with the seroprevalenceof CAEV antibodies, semi-structural questionnaires were developed and presented to farm owners to …
Survey Of Enterococcus Faecalis In Diseased Swine In Hunan Province, China,
2011
Chulalongkorn University
Survey Of Enterococcus Faecalis In Diseased Swine In Hunan Province, China, Wei Jiang, Shi-Cheng Tian, Run-Cheng Li, Yun-Qiu Yan, Ying-Zi Wang, Man-Xiang Li
The Thai Journal of Veterinary Medicine
The prevalence of Enterococcus faecalis was investigated in diseased swine in Hunan Province, China betweenFebruary 2007 and February 2011. A total of 385 diseased swine dissected in prosectorium from 10 representativeadministrative regions in Hunan Province were examined for the presence of E. faecalis. Forty-two out of 385 (10.91%)diseased swine were found to be infected with E. faecalis by bacteria isolations. E. faecalis was mainly recovered fromthe lung and joint cavity. Identification of the organisms was based on biochemical assays and sequencing of thepartial 16S rRNA. The results of the present investigation have implications for the ongoing control of E. faecalisinfections …
Ecg Quiz,
2011
Chulalongkorn University
Ecg Quiz, Chollada Buranakarl
The Thai Journal of Veterinary Medicine
No abstract provided.
