Veterinary Medicine Commons^™

Effect Of Water Temperature On Susceptibility To Streptococcus Agalactiae Serotype Ia Infection In Nile Tilapia (Oreochromis Niloticus), Channarong Rodkhum, Pattanapon Kayansamruaj, Nopadon Pirarat

The Thai Journal of Veterinary Medicine

Streptococcus agalactiae is one of the causative agents associated with warm-water streptococcosis, in whichproduce massive mortality in aquaculture. Emergence of disease in tilapia farm usually occurs in high temperatureseason, which suggested for higher susceptibility of tilapia in this particular condition. Thus, the objective of thisstudy is to investigate the association between water temperature and susceptibility of Nile tilapia (Oreochromisniloticus) to S. agalactiae serotype Ia infection. Nile tilapia were inoculated with 108, 107 or 106 CFU/ml of S. agalactiaeserotype Ia field strain via water immersion route and maintained in different water temperature at 25, 30 or 33oC for1 week. Diseased fish …

Differences Of Periods Of Calving On Days Open Of Dairy Cows In Different Regions And Months Of Thailand, Winai Kaewlamun, Rut Chayaratanasin, Prachin Virakul, Andrew A. Ponter, Patrice Humblot, Siriwat Suadsong, Padet Tummaruk, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The objectives of this study were to analyze the potential impact of heat stress on reproduction in differentregions of Thailand, to determine the monthly distribution of calving throughout the year and to investigateenvironmental sources of variation of days-open (DO) in first lactation dairy cows. Data including 13,548 lactationrecords were collected during 2004 to 2006. Climate data were obtained from the provincial meteorological stationsand the corresponding temperature-humidity indexes were calculated. The difference between regions in THI wasdetermined. The geographical regions studied were: Central, Eastern, Northeastern, and Northern. The distributionof calving by month was determined in the 1st to 5th lactation. The …

Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura

The Thai Journal of Veterinary Medicine

We studied the prevalence of swine salmonellosis and PFGE genotype of isolates in Gunma Prefecture,Japan. Between 2005 and 2008, swine salmonellosis was confirmed in 430 of 2,707,402 (0.02%) swine atslaughterhouses. All isolates were identified as deriving from Salmonella Choleraesuis, biotype Choleraesuis (negativefor H2S production). We used 30 bacterial strains from 15 farms that had experienced outbreaks in 2006 and 2007. Allstrains were susceptible to various antibiotics such as cephems (cefotaxime), fluoroquinolones (norfloxacin andciprofloxacin), and fosfomycin. On the other hand, all strains were resistant to tetracycline (TC), and 29 of 30 (97%)strains were resistant to streptomycin (SM). The most predominant profiles …

Effect Of Roselle (Hibiscus Sabdariffa Linn.) Calyx In Laying Hen Diet On Egg Production Performance, Egg Quality And Tbars Value In Plasma And Yolk, Piyaphon Sukkhavanit, Kris Angkanaporn, Suwanna Kijparkorn

The Thai Journal of Veterinary Medicine

An experiment was conducted to investigate the effect of Roselle calyx in two preparation forms in layerdiets on egg production performance, egg quality and TBARS value in plasma and yolk. Two hundred and seventy,33-week-old, CP Brown laying hens were randomly allocated into 6 treatments with 5 replications of 9 hens each.The dietary treatments were control diet, control diet supplemented with 250 mg/kg α-tocopheryl acetate, dietcontaining 1 and 2% of Roselle calyx crude extracts, and diet containing 2% and 4% Roselle calyx powder. Eggproduction performance was recorded, egg quality was measured and TBARS value in plasma and yolk wereanalyzed after fed …

Effect Of Equex Stm Paste On The Quality And Motility Characteristics Of Post Thawed Cryopreserved Goat Semen, Nitira Anakkul, Junpen Suwimonteerabutr, Jinda Singlor, Nawapen Phutikanit, Theerawat Tharasanit, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The aim of this study was to evaluate the effect of Equex STM Paste on goat semen quality aftercryopreservation. Semen samples were consecutively collected once a week, from six mature bucks. The semensamples with adequate motility (>60%) and sperm concentration (>1,500x106 sperm/ml) were frozen in an egg yolk-Tris semen extender supplemented either with (E+) or without (E-) Equex STM Paste. The semen quality includingmotility, viability, morphology, acrosome integrity and membrane functional integrity were evaluated beforeprocessing and after cryopreservation. Computer-assisted-semen analysis (CASA) was used to characterize the spermmotion patterns during the thermal resistance test at 0, 1, 2 and …

Detection Of West Nile Virus In Mosquitoes In Nakhon-Pathom And Phetchaburi Province, Thailand, Kriangkrai Witoonsatian, Nuananong Sinwat, Rungroj Jam-On, Khongsak Thiangtum, Thaweesak Songserm

The Thai Journal of Veterinary Medicine

West Nile (WN) disease is one of the encephalitic diseases in humans and mammals. This disease has beenreported in several areas of the world. Thailand is located in the area of high risk of WN virus (WNv) infectionconcerning a natural transmission cycle. However, the WNv infection has not been previously reported. Therefore,the aim of this study was to detect WNv in mosquitoes in some areas in Nakhon-pathom and Phetchaburi provinces,central Thailand. The period of this study was done during November 2008 to November 2010. We found that therewere four genera collected including Aedes, Anopheles, Culex and Mansonia. Culex spp such …

Comparison Of Genomic And Proteomic Data In Recurrent Airway Obstruction Affected Horses Using Ingenuity Pathway Analysis, Julien Racine, Vinzenz Gerber, Marybeth Miskovic Feutz, C Paige Riley, Jiri Adamec, June E. Swinburne, Laurent Couetil

Department of Comparative Pathobiology Faculty Publications

Background: Recurrent airway obstruction (RAO) is a severe chronic respiratory disease affecting horses worldwide, though mostly in the Northern hemisphere. Environmental as well as genetic factors strongly influence the course and prognosis of the disease. Research has been focused on characterization of immunologic factors contributing to inflammatory responses, on genetic linkage analysis, and, more recently, on proteomic analysis of airway secretions from affected horses. The goal of this study was to investigate the interactions between eight candidate genes previously identified in a genetic linkage study and proteins expressed in bronchoalveolar lavage fluid (BALF) collected from healthy and RAO-affected horses. The …

Effect Of The Infection With Porcine Reproductive And Respiratory Syndrome Virus On The Regulation Of Cytokines: Tumor Necrosis Factor-Alpha And Interleukin-10, Sakthivel Subramaniam

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Porcine reproductive and respiratory syndrome virus (PRRSV) causes late-term abortion in sows and pneumonia in growing piglets. PRRSV evades the host immune response by several mechanisms, including the modulation of cytokine secretions in infected pigs, which is the subject of this dissertation. Particularly, PRRSV reduces the secretion of the pro-inflammatory cytokine, tumor necrosis factor-α (TNF-α) but increases the secretion of the anti-inflammatory cytokine interleukin-10 (IL-10). The latter effect, however, is PRRSV strain-specific. In this dissertation, we have examined mechanisms by which PRRSV regulates TNF-α and IL-10 expressions. The pathogenic strain FL12, derived from a PRRSV infectious clone, consistently suppressed TNF-α …

Role Of Porcine Reproductive And Respiratory Syndrome Virus Non-Structural Protein 1 In Viral Replications And Pathogenesis, Lalit Kumar Beura

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Porcine reproductive and respiratory syndrome virus (PRRSV) is an infectious agent of significant concern to the global swine industry. PRRSV infection of pigs is initiated by a long viremia after which the virus enters in an extended persistent phase of 3-4 months that ultimately is resolved by the immune system. The delayed and weak host adaptive response is responsible for the protracted convalescence period. An initial sub-optimal innate immune response is postulated to be the reason behind such meager adaptive immune response. A major focus of the studies undertaken in this dissertation was to identify the viral non-structural proteins (nsps) …

Identification Of Host Proteins Required For Vesicular Stomatitis Virus Infection, Debasis Panda

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Viruses usurp host cell pathways for different stages of their infection. Understanding virus-host interaction will be invaluable to elucidate molecular mechanisms of virus infection and to identify drug targets. In order to identify such critical cellular genes in vesicular stomatitis virus (VSV, a model non-segmented negative strand RNA virus) infection, we developed a stable cell line constitutively expressing replication proteins of VSV. Attempts to establish a cell line replicating a sub-genomic replicon was not successful because of induction of interferon response by replication of viral genomic analog. Subsequently, we used siRNA technology and conducted a genome-wide siRNA screen in HeLa …

Glutaredoxin 2 Prevents H2o2-Induced Cell Apoptosis By Protecting Complex I Activity In The Mitochondria, Hongli Wu, Kuiyi Xing, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutaredoxin 2 (Grx2) belongs to the oxidoreductase family and is an isozyme of glutaredoxin 1 (Grx1) present in the mitochondria, however its function is not well understood. The purpose of this study is to evaluate the potential anti-apoptotic function of Grx2 by examining its ability to protect complex I in the mitochondrial electron transport system using human lens epithelial cells as a model. We found that cells treated with 200 μM hydrogen peroxide (H2O2) for 24 h exhibited decreased viability and became apoptotic with corresponding Bax up-regulation, Bcl-2 down-regulation, caspase 3 activation and mitochondrial cytochrome c leakage. Grx2 over-expression (OE) …

Regulation Of Cytosolic Phospholipase A2 (Cpla2) And Its Association With Cell Proliferation In Human Lens Epithelial Cells, Yin Wang, Kui-Yi Xing, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

PURPOSE. To investigate the molecular mechanism for cytosolic phospholipase A2 (cPLA2) regulation and its association to platelet-derived growth factor (PDGF)-induced cell proliferation.

METHODS. cPLA2 was examined using human lens epithelial (HLE) B3 cells. Reactive oxygen species (ROS) generation induced by PDGF was analyzed by luminescence assay. Cell proliferation was measured by cell counting and by BrdU assay. Human cPLA2 gene was cloned via RT-PCR followed by sitedirected mutagenesis to construct HLE B3 cells expressing either inactive cPLA2 enzyme with S228A mutation (S228A), or cPLA2 truncated at the calcium-binding C2 domain (C2D). Activity of cPLA2 was measured by arachidonic acid (AA) …

Animal Health Matters, David H. Zeman, Russ Daly

Animal Health MATTERS Newsletter

[Page] 1- ADRDL Hosts North Central Conference of Veterinary Lab Diagnosticians [Page] 2- Director's Message [Page] 2- SDSU Extension Veterinarian Designated as State Health Veterinarian [Page] 3- ADRDL Virology Section Recognized by SD Game, Fish and Parks [Page] 3- Summery of BVDV-PI Testing at SDSU ADRDL, FY2010 [Page] 4- The Veterinary and Biomedical Sciences Clerical Team: The Support Behind Department Missions [Page] 5- Veterinary and Biomedical Sciences Faculty, Pre-Vet Students Explore Veterinary Education in New Zealand Pieces and Parts [Page] 6- Griffith Undergraduate Research Award Won by SDSU Pre-Veterinary Student [Page] 6- Matthees Recognized with Outstanding Service Award [Page] 6- …

The Antinociceptive Effects Of Tramadol On The Thermal Threshold Response In Cats, Saowanee Jiwlawat, Sumit Durongphongtorn

The Thai Journal of Veterinary Medicine

The study is of the antinociceptive effects of tramadol on the thermal threshold response in eight cats using athermal threshold-testing device which contained a heater element and a temperature sensor placed on the shavedlateral thoracic area. Each cat randomly received an intramuscular administration of tramadol 2 mg/kg, morphine 0.2mg/kg and saline 0.04 ml/kg (placebo) at a weekly interval from an observer unaware of the treatment. The thermalthresholds were measured and recorded by activation of the heater until the cat showed a positive response (e.g. skinflicks, turning and looking at the probe and jumping forwards). Three baseline measurements were made at …

Prevalence Of Mycoplasma Wenyonii Infection On Seven Dairy Farms In Shanghai, China, Yuan Congli, Ying Hong, Zhang Zhonghai, Yang Zhibiao, Cui Li, Zhu Jianguo, Hua Xiuguo

The Thai Journal of Veterinary Medicine

Mycoplasma wenyonii (M. Wenyonii) was studied by PCR and blood smear staining in 197 cows. Clinical signsand hematological parameters of all tested cows were collected. Results confirmed that 31% populations werepositive of M. wenyonii infection. Statistical analysis revealed that the cows of 2-4 years old were most susceptible. Noclinical sings were found to be significantly associated with the infection. The infection also did not result in anemiabased on hematological investigation that only showed elevation of WBC counts in infected cows. Sequence analysisof the partial 16S rRNA gene showed that three different representative strains were confirmed in the present study.

Cloning And Analysis Of Pirnas From Testes Of Three Species, Guobin Chang, Rong Chen, Ying Zhang, Aiqin Dai, Teng Ma, Guohong Chen

The Thai Journal of Veterinary Medicine

piRNAs are a testis-specific class of small RNAs in mammal. Sixteen sequences of piRNAs were cloned fromthree different species by the method of construction of cDNA library including two sequences from mouse, eightsequences from quail and six sequences from chicken. Herein, the two piRNAs sequences from mouse had alreadybeen identified, while the others were cloned for the first time. The sequence analysis indicated that the avian piRNAshad a similar length and a preference of uridine for 5’-end with varying degrees to the mammalian piRNAs. Besidethe function in the process of sperm development and maturation, avian piRNAs may also have a …

Evaluation Of Cardboard Coated With Natural Substances In Combination With Ink On Rat Repellency, Sarinee Kalandakanond-Thongsong, Suwaporn Daendee, Boonrit Thongsong, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

An effective repellent for rodent-proof cardboard-container is considered interesting especially in packagingand transport business as rat usually gnaws through the container, resulting in food deterioration or goods loss.Rodenticide usage is a common approach for rodent control; however, most rodenticides are poisonous to man aswell as to the rats. Therefore, preferably the usage of non-toxic rat repellent is an interesting alternative. In this study,various natural extracts, i.e. wintergreen oil, chilli, peppermint oil, bergamot oil and geranium oil, which had beenshown to repel rats when apply singly or in combination, were mixed with ink, coated on cardboard before beingplaced in circular open …

Respiratory Hypocapnia At Different Stages Of Lactation During Long-Term Exogenous Bovine Somatotropin In Crossbred Holstein Cattle In The Tropic, Sumpun Thammacharoen, Siripen Komolvanich, Somchai Chanpongsang, Narongsak Chaiyabutr

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the influence of long-term administration of recombinant bovinesomatotropin (rbST) on the acid-base homeostasis of lactating crossbred cows under hot and humid condition ofThailand. Two groups of five animals each of primiparous crossbred dairy cattle were supplemented either with rbSTor with control vehicle every 14 days throughout the period of lactation. The blood gas parameters, milk yield andfeed intake from both groups were recorded at each stage of lactation. Cows supplemented with rbST showed milddegree of acid-base imbalance during early to mid lactation. The lower partial pressure of carbon dioxide (paCO2) andplasma bicarbonate …

Chikungunya Virus In Thailand; An Update, Sonthaya Tiawsirisup

The Thai Journal of Veterinary Medicine

No abstract provided.

Genes Associated With Genetic Diseases In Collies, Shetland Sheepdogs And Border Collies, Gunnaporn Suriyaphol

The Thai Journal of Veterinary Medicine

Canine genetic disorders inherited through genes or chromosomes. Mutations can also occur spontaneouslyor influenced by environmental factors. Such disorders can be transmitted to offspring. However, the disease may notbe developed owing to other influenced factors. Dominant and recessive genetic mechanisms also affect the traitoutcome. This article discussed the abnormalities of genes affected by the disease and/ or genetic disorders in Collies,Shetland sheepdogs and Border collies, sharing the Collie lineage. Most of these disorders are caused by autosomalrecessive genes. Hence, paired recessive alleles are required in order to express its phenotype. Although dogs areasymptomatic carriers, they can pass down defective genes …