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Acemannan/Alginate Composite Hydrogels Promote Sto Cell Spreading And Metabolic Activity, Hong Yongxing, Tharasanit Theerawat 2026 Faculty of Veterinary Science, Chulalongkorn University

Acemannan/Alginate Composite Hydrogels Promote Sto Cell Spreading And Metabolic Activity, Hong Yongxing, Tharasanit Theerawat

The Thai Journal of Veterinary Medicine

Alginate-based hydrogels are widely used as biocompatible scaffolds. However, their limited cell-adhesive properties restrict their application in tissue engineering. This study aimed to fabricate acemannan/alginate (ACE/ALG) composite hydrogels and evaluate their cytocompatibility and ability to support STO cell spreading and metabolic activity. Composite hydrogels were prepared by blending different concentrations of ACE with alginate, followed by Ca²⁺-mediated ionic crosslinking. Rheological analysis showed that ACE incorporation increased both the storage modulus (G′) and loss modulus (G″) of the hydrogels. Cell viability and morphology were evaluated using Live/Dead staining on day 7, and cellular growth was assessed using an MTT assay on …


Effects Of Microalgae Supplementation On Broiler Meat Quality, Antioxidant Status, And Fatty Acid Profile, Ebrar Filizoğlu, Özlem Karadağoğlu 2026 Institute of Health Sciences, Kafkas University

Effects Of Microalgae Supplementation On Broiler Meat Quality, Antioxidant Status, And Fatty Acid Profile, Ebrar Filizoğlu, Özlem Karadağoğlu

The Thai Journal of Veterinary Medicine

This study aimed to evaluate the dose-dependent effects of dietary supplementation with Schizochytrium limacinum, a microalga rich in omega-3 fatty acids and bioactive compounds, on the fatty acid composition and antioxidant status of broiler breast meat, as well as its potential effects on growth performance, carcass characteristics, and meat quality. In a 42-day feeding trial, 192 day-old Ross-308 broiler chicks were randomly allocated to four dietary treatment groups (48 chicks per group): a control group fed the basal diet without S. limacinum supplementation (0 g/kg), and three treatment groups fed the basal diet supplemented with 0.25, 0.5, and 1 …


Reference Values For Intraocular Pressure In Collared Scops Owls (Otus Lettia) Measured Using Rebound Tonometry, Yun-Shan Chiu, Chau-Hwa Chie, Pin-Huan Yu, Chung-Tien Lin 2026 School of Veterinary Medicine, National Taiwan University

Reference Values For Intraocular Pressure In Collared Scops Owls (Otus Lettia) Measured Using Rebound Tonometry, Yun-Shan Chiu, Chau-Hwa Chie, Pin-Huan Yu, Chung-Tien Lin

The Thai Journal of Veterinary Medicine

In this study, we aimed to establish species-specific reference values for intraocular pressure (IOP) in clinically healthy collared scops owls (Otus lettia) using rebound tonometry. Twelve free-living owls were included, and twentyfour eyes were examined. All owls were physically restrained in an upright position without sedation. Intraocular pressure was measured in both eyes using rebound tonometry (TONOVET; iCare Finland Oy, Vantaa, Finland) in d mode. No significant difference in IOP was observed between the right and left eyes. The mean IOP values were 10.42 ± 1.24 mmHg (median, 11 mmHg; range, 8–12 mmHg) in the right eye and 10.75 ± …


Hypovitaminosis A - Associated Neurologic Syndrome With Concurrent Babesiosis In A Captive African Lion Cub (Panthera Leo), Kano, Nigeria, Shaarani Habibu Musa, Aminu Bello Hanga, Auwal Usman Nagoma, Caleb Ayuba Kudi, Paul Ayuba Abdu, Musa Ibrahim Waziri, Haruna Baba Galadima, Yusuf Aminu Yusuf, Muhammed Mikail 2026 Ministry for Livestock Development, Kano State

Hypovitaminosis A - Associated Neurologic Syndrome With Concurrent Babesiosis In A Captive African Lion Cub (Panthera Leo), Kano, Nigeria, Shaarani Habibu Musa, Aminu Bello Hanga, Auwal Usman Nagoma, Caleb Ayuba Kudi, Paul Ayuba Abdu, Musa Ibrahim Waziri, Haruna Baba Galadima, Yusuf Aminu Yusuf, Muhammed Mikail

The Thai Journal of Veterinary Medicine

Neurological disorders in captive wild felids presents diagnostic and therapeutic challenges, particularly in juveniles animals maintained under suboptimal nutritional transitions. This case report describes the synergistic effects of dietary deficiencies and a concurrent hemoparasitic infection in a seven-month-old male African lion cub (Panthera leo) weighing 30 kg, housed at Audu Bako Zoological Garden in Kano, Nigeria. Following premature weaning onto an unsupplemented muscle-meat diet, the cub exhibited progressive neurological symptoms, including ataxia, hindlimb paresis, star-gazing, and opisthotonos. Hematological analysis revealed a mild normocytic, normochromic anemia (packed cell volume; 23%) and the presence of erythrocytes rouleaux formation, while Giemsa-stained …


Preliminary Development Of Visual And Real-Time Loop-Mediated Isothermal Amplification Assays For Megalocytivirus Detection In Betta Fish (Betta Splendens), Kitikarn Sakuna, Supaporn Noochu, Kittichon U-Taynapun, Nion Chirapongsathonkul, Saransiri Nuanmanee 2026 Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya

Preliminary Development Of Visual And Real-Time Loop-Mediated Isothermal Amplification Assays For Megalocytivirus Detection In Betta Fish (Betta Splendens), Kitikarn Sakuna, Supaporn Noochu, Kittichon U-Taynapun, Nion Chirapongsathonkul, Saransiri Nuanmanee

The Thai Journal of Veterinary Medicine

This study developed rapid detection of Megalocytivirus using visual and real-time loop-mediated isothermal amplification (LAMP) assays. The primer was designed with the major capsid protein gene of Megalocytivirus, including red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV), and turbot reddish body iridovirus (TRBIV). The visual and real-time LAMP assays were evaluated using DNA extracted from symptomatic betta fish that tested negative for Megalocytivirus by the broad-range PCR assay, as well as a chemically synthesized Megalocytivirus DNA template. Visual LAMP was found sensitive enough to detect up to 10 pg per reaction of the target DNA …


Application Of Flexible Endoscopy For Gastrointestinal Foreign Body Removal In A Leopard Gecko (Eublepharis Macularius), Jang-Hee Han, Seung Yoon Ahn, Do Na Lee, Seong Chan Yeon 2025 College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University

Application Of Flexible Endoscopy For Gastrointestinal Foreign Body Removal In A Leopard Gecko (Eublepharis Macularius), Jang-Hee Han, Seung Yoon Ahn, Do Na Lee, Seong Chan Yeon

The Thai Journal of Veterinary Medicine

A male captive-bred leopard gecko (Eublepharis macularius) presented with a history of inadvertent ingestion of a rubber tip from forceps during feeding. The foreign body was impalpable on physical examination, while radiographic evaluation confirmed its presence in the stomach. Considering the procedural risks associated with invasive surgical interventions, endoscopic retrieval under general anesthesia was pursued. An initial attempt using a 2.8 mm superfine flexible endoscope with 0.8 mm forceps was unsuccessful due to insufficient stability, which hindered effective navigation to the foreign body. In contrast, a second attempt using a larger 9.2 mm flexible endoscope with 2.3 mm …


Effects Of Hambeles (Myrtus Communis L.) Leaf On In Vitro Ruminal Fermentation Parameters, Methane Production And Protozoa Counts, Çağrı Kale, Fatma Özkan, Taylan Aksu, Devrim Saripinar Aksu, Nuriye Tuğba Bingöl, Mehtap Güney, Süleyman Ercüment Önel 2025 Van Yuzuncu Yil University, Turkey

Effects Of Hambeles (Myrtus Communis L.) Leaf On In Vitro Ruminal Fermentation Parameters, Methane Production And Protozoa Counts, Çağrı Kale, Fatma Özkan, Taylan Aksu, Devrim Saripinar Aksu, Nuriye Tuğba Bingöl, Mehtap Güney, Süleyman Ercüment Önel

The Thai Journal of Veterinary Medicine

Current ruminant nutrition studies are based on improving rumen fermentation to enhance performance and reduce greenhouse gases. For this purpose, the use of herbal-based feed supplements has become widespread. To determine the effects of adding Hambeles (Myrtus communis L.) leaf to some roughage on rumen fermentation parameters by in vitro gas production technique. Corn silage, alfalfa hay and wheat straw were used as roughage. Hambeles leaf was added to roughage at the rate of 0%, 3%, 6%, 9% of the dry matter. According to the data obtained, it was determined that adding 6% and 9% Hambeles leaves to corn …


Bacterial Identification And Antibiotic Sensitivity Profiles From Captive Birds At Taipei Zoo, Younjung Chang, I-Li Liu, Jane-Fang Yu, Lih-Chiann Wang 2025 Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University

Bacterial Identification And Antibiotic Sensitivity Profiles From Captive Birds At Taipei Zoo, Younjung Chang, I-Li Liu, Jane-Fang Yu, Lih-Chiann Wang

The Thai Journal of Veterinary Medicine

Bacterial infections are common in zoo birds. They may be caused by pathogenic bacteria or opportunistic bacteria due to trauma or stress-induced immune compromise. Improper use of antibiotics contributes to the development of resistant bacteria, making treatment complicated or ineffective. However, comprehensive clinical investigations into bacterial prevalence and antibiotic susceptibility in zoo-captive birds remain limited. This study identified the bacterial species present in captive diseased birds at the zoo and conducted a 12-year analysis of their antibiotic susceptibility profiles. The specimens from 48 birds at Taipei Zoo from 2006 to 2017 were submitted to a commercial reference laboratory for bacterial …


Region-Specific Hematological And Biochemical Reference Intervals For Southern Thai Fighting Bulls, Dhiravit Chantip, Nantaporn Chooruang, Kitikarn Sakuna, Niramol Thongtem, Wiruntita Bohman 2025 Department of Veterinary Bio-Medical Sciences, Faculty of Veterinary Sciences, Rajamangala University of Technology Srivijaya, Nakhon Si Thammmarat, Thailand

Region-Specific Hematological And Biochemical Reference Intervals For Southern Thai Fighting Bulls, Dhiravit Chantip, Nantaporn Chooruang, Kitikarn Sakuna, Niramol Thongtem, Wiruntita Bohman

The Thai Journal of Veterinary Medicine

Accurate health assessments are vital for the performance and welfare of fighting bulls. Existing hematological and biochemical reference intervals (RIs) for cattle may not adequately reflect the unique characteristics of fighting bulls due to breed, age, and geographical differences. This study aimed to develop and apply region-specific RIs for hematological and biochemical parameters in fighting bulls from southern Thailand. A retrospective analysis of laboratory records was conducted on 1,460 healthy fighting bulls enrolled in an annual health check program at the Livestock Animal Hospital, Faculty of Veterinary Sciences, Rajamangala University of Technology Srivijaya, from January 2016 to December 2023. Blood …


Assessing The Feasibility Of Microneedle Patches For Gnrh Immunocontraception In Mixed-Breed Dogs (Canis Familiaris), Ai-Mei Chang, Hsiao-Hui Huang, Ding-Liang Hou, Zi-Yu Lin, Mei-Chin Chen, Chen-Chih Chen, Jai-Wei Lee 2025 University of Tasmania

Assessing The Feasibility Of Microneedle Patches For Gnrh Immunocontraception In Mixed-Breed Dogs (Canis Familiaris), Ai-Mei Chang, Hsiao-Hui Huang, Ding-Liang Hou, Zi-Yu Lin, Mei-Chin Chen, Chen-Chih Chen, Jai-Wei Lee

The Thai Journal of Veterinary Medicine

The overpopulation of free-roaming dogs poses significant risks to endemic species, public health, and the environment through predation and disease transmission. While surgical castration is effective, it raises ethical concerns regarding animal welfare. Immuno-castration offers a humane and effective alternative for population control, but a more easily administered and cost-efficient method is needed to enable widespread vaccination efforts. In this study, we aimed to employ self-dissolving microneedle patches for a simple and potentially cost-effective immunization of a recombinant GnRH-based vaccine to control fertility. Eight mongrel dogs were randomly divided into 2 groups (n=4 each) and immunized with the recombinant GnRH-based …


Local Tissue Damage Treatment Of Snake Venom, Suchitra Khunsap 2025 Research and Development Department, Queen Saovabha Memorial Institute

Local Tissue Damage Treatment Of Snake Venom, Suchitra Khunsap

The Thai Journal of Veterinary Medicine

Local tissue damage caused by snake envenomation was a medical problem that caused morbidity and mortality for more than a decade, with no defined treatment guidelines. Nowadays, antivenom is the primary treatment for snakebite, which substantially reduces systemic venom but not significantly reducing local symptoms. We compiled a list of local damage therapies, including antivenom medication, surgery and skin grafting, small molecule medicines, and alternative therapies. Although each was not approved as a standard treatment, it may be a treatment option for tissue damage caused by snake venom. However, antivenoms and most resistant compounds may not be the best strategy …


Morphological And Scanning Electron Microscopy Characterization Of A Spirurid In A Wild Sunda Pangolin (Manis Javanica), Punnarat Vibulchan, Chong Shin Min, Chiharu Okumura, Piyanan Taweethavonsawat 2025 Chulalongkorn University

Morphological And Scanning Electron Microscopy Characterization Of A Spirurid In A Wild Sunda Pangolin (Manis Javanica), Punnarat Vibulchan, Chong Shin Min, Chiharu Okumura, Piyanan Taweethavonsawat

The Thai Journal of Veterinary Medicine

This study identifies and characterizes a spirurid in a wild Sunda pangolin (Manis javanica) using scanning electron microscopy (SEM). Native to Southeast Asia, the Sunda pangolin faces critical threats from poaching, illegal trafficking for its scales and meat, habitat loss, urban conflicts, and disease. Despite this, little is known about the biology of wild Sunda pangolins. In this research, spirurid parasites were recovered from a pangolin fatally injured in a vehicular collision. The parasites were preserved and analyzed with SEM, revealing key morphological features such as papillae, lip-like structures, and an anal opening in females. This study enhances …


A Two-Hit Model Of Executive Dysfunction: Simulated Galactic Cosmic Radiation Primes Latent Deficits Revealed By Sleep Fragmentation, Richard A. Britten, Ella N. Tamgue, Paola Arriaga Alvarado, Arriyam S. Fesshaye, Larry D. Sanford 2025 Macon & Joan Brock Virginia Health Sciences at Old Dominion University

A Two-Hit Model Of Executive Dysfunction: Simulated Galactic Cosmic Radiation Primes Latent Deficits Revealed By Sleep Fragmentation, Richard A. Britten, Ella N. Tamgue, Paola Arriaga Alvarado, Arriyam S. Fesshaye, Larry D. Sanford

Department Radiation Oncology & Biophysics Faculty Publications

Future Artemis-class missions to Mars will expose astronauts to prolonged space radiation (SR), sleep disruption, and operational demands requiring greater autonomy, placing decision making and executive function at heightened risk. Both SR and sleep fragmentation (SF) independently impair cognition, yet their combined effects remain poorly understood. Using the Associative Recognition Memory and Interference (ARMIT) task, we assessed cognitive performance in male rats exposed to 10 cGy of Galactic Cosmic Ray simulation (GCRsim), SF, or both. Under well-rested conditions, GCRsim-exposed rats exhibited overt deficits in the C.1.2 stage, performing at chance when reinforcement contingencies shifted, consistent with impaired cognitive flexibility. In …


Diagnosis And Treatment Of Crop Inflammation Of Rough-Legged Buzzard (Buteo Lagopus) In Daqing Wildlife Nature Reserve, Heilongjiang Province, China, Wang Zheng, Ding Ruxin, Ge Yansong, Xu Enshuang, Zheng Jiasan 2024 Heilongjiang Bayi Agricultural University

Diagnosis And Treatment Of Crop Inflammation Of Rough-Legged Buzzard (Buteo Lagopus) In Daqing Wildlife Nature Reserve, Heilongjiang Province, China, Wang Zheng, Ding Ruxin, Ge Yansong, Xu Enshuang, Zheng Jiasan

The Thai Journal of Veterinary Medicine

This article describes a Rough-legged Buzzard (Buteo lagopus) from the Wildlife Nature Reserve in Daqing, China. According to the staff of Daqing Wildlife Nature Reserve, the Rough-legged Buzzard was found to have impaired flight ability, depression, and loss of aggression, but its feathers were full and there were no obvious external injuries, so it was sent to the Wildlife Rehabilitation Center of the Teaching Animal Hospital of Heilongjiang Bayi Agricultural University for treatment. After the vet conducted X-ray and blood tests, he diagnosed the bird with a foreign body in the crop and crop inflammation. He finally treated …


Hematology, Blood Biochemistry And Cytochemical Characteristics Of Blood Cells Of Captive Aldabra Giant Tortoise (Aldabrachelys Gigantea) In Thailand, Thanida Haetrakul, Nitiwadee Keschumras, Sirawich Srisiri, Narinthip Chaiyanont, Nisama Wongnitipat, Phatcharaporn Phuasombatcharoen, Praewa Sirichitr, Nantarika Chansue 2024 Veterinary Medical Aquatic Animal Research Center of Excellence, Faculty of Veterinary Science, Chulalongkorn University

Hematology, Blood Biochemistry And Cytochemical Characteristics Of Blood Cells Of Captive Aldabra Giant Tortoise (Aldabrachelys Gigantea) In Thailand, Thanida Haetrakul, Nitiwadee Keschumras, Sirawich Srisiri, Narinthip Chaiyanont, Nisama Wongnitipat, Phatcharaporn Phuasombatcharoen, Praewa Sirichitr, Nantarika Chansue

The Thai Journal of Veterinary Medicine

Aldabra giant tortoises (Aldabrachelys gigantea), well-known pet tortoises worldwide, were classified as Appendix II by The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). This study aims to investigate the hematological, blood biochemical, and cytochemical characteristics of blood cells in healthy Aldabra giant tortoises in captivity. Blood samples were collected from the dorsal tail vein of 12 tortoises (6 males and 6 females) during May and October 2022. The hematological values showed no statistically significant differences between male and female tortoises. However, in female tortoises, calcium and phosphorus levels were significantly higher than …


Distinct Effects Of Extra- And Intracellular Trehalose Supplementation In Freezing Medium On The Post-Thaw Developmental Capacity Of Feline Oocytes, Saengtawan Arayatham, Wirakan Kallayanathum, Panuwat Padungros, Theerawat Tharasanit 2024 Faculty of Veterinary Science, Chulalongkorn University

Distinct Effects Of Extra- And Intracellular Trehalose Supplementation In Freezing Medium On The Post-Thaw Developmental Capacity Of Feline Oocytes, Saengtawan Arayatham, Wirakan Kallayanathum, Panuwat Padungros, Theerawat Tharasanit

The Thai Journal of Veterinary Medicine

Oocyte cryopreservation is a crucial technique for preserving the genetic material of female cats, yet maintaining post-thaw oocyte developmental competence remains a significant challenge. This study investigated the impact of intra- and extracellular trehalose on the developmental competence of feline oocytes following cryopreservation. In Experiment 1, immature feline oocytes were matured in vitro (IVM) with varying concentrations (0, 1.5, and 3 mM) of intracellular trehalose hexaacetate (Tre-(OAc)6). The matured oocytes were then cryopreserved and thawed prior to in vitro fertilization and embryo culture. Experiment 2 examined the effects of extracellular trehalose supplementation at concentrations of 0, 0.125, 0.25, and 0.5 …


Interaction Of Cumulus-Oocyte Complex (Coc) Number, Oocyte Quality, And Blastocyst Numbers By Repeated Ovum Pick-Up (Opu) In In Vitro Embryo Production, Sakine Ülküm Çizmeci, Dursun Ali Dinc, Mustafa Numan Bucak, Ömer Faruk Yesilkaya, Muhammed Furkan Ciftci, Mehmet Buğra Kivrak, Ayşe Sari, Hasan Ali Cay, Maide Golbasi, Mustafa Bodu 2024 University of Selcuk

Interaction Of Cumulus-Oocyte Complex (Coc) Number, Oocyte Quality, And Blastocyst Numbers By Repeated Ovum Pick-Up (Opu) In In Vitro Embryo Production, Sakine Ülküm Çizmeci, Dursun Ali Dinc, Mustafa Numan Bucak, Ömer Faruk Yesilkaya, Muhammed Furkan Ciftci, Mehmet Buğra Kivrak, Ayşe Sari, Hasan Ali Cay, Maide Golbasi, Mustafa Bodu

The Thai Journal of Veterinary Medicine

Ovum pick-up (OPU) is a repeatable technique that is used for the retrieval of large numbers of immature oocytes from the antral follicles of live animals. The aim of the present study was to determine the effect of repeated OPU application on cumulus-oocyte complex (COC) number, oocyte quality, and blastocyst rates. The animal material for the study consisted of 10 Holstein heifers. Heifers selected as donors were administered OPU a total of 9 times on random days of the cycle at 1-week intervals. All antral follicles with a diameter of 2-8 mm were aspirated during the OPU procedure. In vitro …


Alabama Agricultural Producers’ Experiences With Wild Pigs (Sus Scrofa) And Their Preferences Concerning Wild Pig Management, Hailey E. Ellis, Luren M. Jaebker, Alan D. Bright, Mark D. Smith, Keith M. Carlisle 2024 Colorado State University

Alabama Agricultural Producers’ Experiences With Wild Pigs (Sus Scrofa) And Their Preferences Concerning Wild Pig Management, Hailey E. Ellis, Luren M. Jaebker, Alan D. Bright, Mark D. Smith, Keith M. Carlisle

United States Department of Agriculture Wildlife Services: Staff Publications

Government agencies and landowners use a variety of lethal methods to reduce invasive wild pig populations and resulting agricultural damages. To provide decision-makers with information regarding Alabama farmers’ experiences with wild pigs and their preferences for management actions, we surveyed Alabama Farmers Federation members in 2020. We found that wild pigs were commonly present on livestock, timber, and crop production acreage, but wild pig damages and costs were much more common among crop producers. Respondents' wildlife acceptance capacity for wild pigs was low and all lethal control methods were considered aceptable by most reposndents, particularly those performed by landowners. While …


Presynchronization In Sheep Ensures Synchronization Of Next Estrus And Improves Fertility, Bülent Bülbül, Mehmet Köse, Mesut Kırbaş, Kenan Çoyan, Mehmet B. Ataman, Emine A. Bekçi, Seyit Ümütlü, Uğur Demirci, Bumin E. Teke 2024 Dokuz Eylul University

Presynchronization In Sheep Ensures Synchronization Of Next Estrus And Improves Fertility, Bülent Bülbül, Mehmet Köse, Mesut Kırbaş, Kenan Çoyan, Mehmet B. Ataman, Emine A. Bekçi, Seyit Ümütlü, Uğur Demirci, Bumin E. Teke

The Thai Journal of Veterinary Medicine

In this study, fertility was evaluated either in the first hormonally synchronized or in the subsequent natural estrus among 202 sheep during the breeding season. Intramuscular (im) PGF2α was administered 11 days apart in both the PG (n=50) and Pre-PG (n=50) groups, while im PGF2α was given at the removal of an intravaginal progesterone sponge (after 11 days) in the Sponge (n=51) and Pre-Sponge (n=51) groups. Estrus was monitored following the last hormonal application (day 0) in all groups and starting from day 16 in the Pre-PG and Pre-Sponge groups for 5 days. Ewes were mated during the first hormonally …


Development Of A Silicone-Based Goat Uterine Model For Artificial Insemination Training, Sakdichod Kimsakulvech 2024 Mahidol University

Development Of A Silicone-Based Goat Uterine Model For Artificial Insemination Training, Sakdichod Kimsakulvech

The Thai Journal of Veterinary Medicine

A goat uterine model for artificial insemination (AI) training has not been created previously. The eight goat uteri were collected from the slaughterhouse and measured to serve as a prototype size for the model. A goat uterine model was developed based on silicone material. This study compared the suitability of silicone between 20,000 and 30,000 centipoise viscosity and the cervical opening between the circular and linear types for this model. These were made up of four models with alternating differences between viscosity and cervical openings. Twenty AI officers evaluated the satisfaction score of the internal and external appearance of the …


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