Genome Of Mycoplasma Haemofelis, Unraveling Its Strategies For Survival And Persistence,
2011
Purdue University
Genome Of Mycoplasma Haemofelis, Unraveling Its Strategies For Survival And Persistence, Andrea P. Santos, Ana Ms Guimaraes, Naila C. Do Nascimento, Phillip J. Sanmiguel, Samuel W. Martin, Joanne Messick
Department of Comparative Pathobiology Faculty Publications
Mycoplasma haemofelis is a mycoplasmal pathogen (hemoplasma) that attaches to the host’s erythrocytes. Distributed worldwide, it has a significant impact on the health of cats causing acute disease and, despite treatment, establishing chronic infection. It might also have a role as a zoonotic agent, especially in immunocompromised patients. Whole genome sequencing and analyses of M. haemofelis strain Ohio2 was undertaken as a step toward understanding its survival and persistence. Metabolic pathways are reduced, relying on the host to supply many of the nutrients and metabolites needed for survival. M. haemofelis must import glucose for ATP generation and ribose derivates for …
Infectious Cdna Clone Of The Modified Live Virus Vaccine Strain Of Equine Arteritis Virus,
2011
University of Kentucky
Infectious Cdna Clone Of The Modified Live Virus Vaccine Strain Of Equine Arteritis Virus, Udeni B. R. Balasuriya, Peter J. Timoney, Jianquan Wang
Veterinary Science Faculty Patents
An isolated polynucleotide molecule includes a DNA sequence encoding an infectious RNA molecule encoding a modified live viral strain of an Equine arteritis virus, wherein the DNA sequence is SEQ ID NO:1 or a degenerate variant thereof. Also provided are transformed or transfected host cells including that sequence, vectors including the sequence, and isolated infectious RNA molecules encoded by the sequence. Further, a modified DNA sequence encoding an infectious RNA molecule encoding a modified live viral strain of an Equine arteritis virus is provided wherein the DNA sequence is SEQ ID NO:2 or a degenerate variant thereof, including a silent …
The Efficacy Of Three Types Of Analgesic Drugs In Reducing Pain In The Rainbow Trout, Oncorhynchus Mykiss,
2011
University of Liverpool
The Efficacy Of Three Types Of Analgesic Drugs In Reducing Pain In The Rainbow Trout, Oncorhynchus Mykiss, Jessica J. Mettam, Lois J. Oulton, Catherine R. Mccrohan, Lynne U. Sneddon
Veterinary Science and Medicine Collection
Recent research has shown the possibility of pain perception in fish; therefore, the use of analgesia or “painkillers” should be considered for invasive procedures. However, there is relatively little information on the effectiveness of analgesic drugs nor on the appropriate dose for fish. This study assessed the efficacy of three types of drug: an opioid, buprenorphine, a non-steroidal anti-inflammatory drug (NSAID), carprofen and a local anaesthetic, lidocaine. Each drug was tested at three doses on rainbow trout that were noxiously stimulated and the most effective dose was also given to fish experiencing no pain to investigate side-effects. Ventilation rate and …
Evaluation Of Microsatellite Polymorphism And Genetic Variability In Thai Ridgeback And Bangkaew Dogs,
2011
Chulalongkorn University
Evaluation Of Microsatellite Polymorphism And Genetic Variability In Thai Ridgeback And Bangkaew Dogs, Janjira Phavaphutanon, Sudtisa Laopiem
The Thai Journal of Veterinary Medicine
The origin of dogs came from the limited number of founders leading to inbreeding or genetic diseasewithin or among breeds. Thai Ridgeback (TR) and Bangkaew (BK) dogs were the only native dog breeds that webelieved to originate in Thailand. The study of genetic variability in Thai dogs (39 Thai Ridgeback dogs, 82 Bangkaewdogs) was defined by using 12 microsatellite markers. These DNA markers were highly informative and distributedthrough the canine genome. The result from these markers revealed the higher number of alleles, observedheterozygosity (Ho), expected heterozygosity (He) and polymorphic information content (PIC) in TR and BK dogs.The average Ho, He …
Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct,
2011
Chulalongkorn University
Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai
The Thai Journal of Veterinary Medicine
The glycosaminoglycans (GAGs) found in the mammalian oviductal tissue and luminal secretory fluidinfluence the fertilization processes in various patterns such as sperm storage, viability and capacitation. The aim ofthis study was to confirm the appearance of hyaluronan and syndecan-1 in the swamp buffalo oviduct consisting ofuterotubal junction (UTJ), isthmus, ampulla and infundibulum during oestrous cycle using histochemical andimmunohistochemical techniques. The non-sulphated GAGs, i.e. hyaluronan (HA), were noticeably localized in thesubepithelial connective tissue layer or lamina propria of all segments while the epithelial HA-positive staining wasdemonstrated only in the UTJ and isthmus (sperm reservoir) at the follicular phase. In contrast, the …
Ecg Quiz,
2011
Chulalongkorn University
Ecg Quiz, Chollada Buranakarl
The Thai Journal of Veterinary Medicine
No abstract provided.
Ophthalmology Snapshot,
2011
Chulalongkorn University
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
What Is Your Diagnosis,
2011
Chulalongkorn University
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep
The Thai Journal of Veterinary Medicine
No abstract provided.
Antagonistic Effects Of Cellular Poly(C) Binding Proteins
On Vesicular Stomatitis Virus Gene Expression,
2011
University of Nebraska-Lincoln
Antagonistic Effects Of Cellular Poly(C) Binding Proteins On Vesicular Stomatitis Virus Gene Expression, Phat X. Dinh, Lalit Beura, Debasis Panda, Anshuman Das, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
Immunoprecipitation and subsequent mass spectrometry analysis of the cellular proteins from cells expressing the vesicular stomatitis virus (VSV) P protein identified the poly(C) binding protein 2 (PCBP2) as one of the P protein-interacting proteins. To investigate the role of PCBP2 in the viral life cycle, we examined the effects of depletion or overexpression of this protein on VSV growth. Small interfering RNA-mediated silencing of PCBP2 promoted VSV replication. Conversely, overexpression of PCBP2 in transfected cells suppressed VSV growth. Further studies revealed that PCBP2 negatively regulates overall viral mRNA accumulation and subsequent genome replication. Coimmunoprecipitation and immunofluorescence microscopic studies showed that …
Acute Fatty Liver Of Pregnancy: An Update On Mechanisms,
2011
University of Nebraska-Lincoln
Acute Fatty Liver Of Pregnancy: An Update On Mechanisms, Sathish Kumar Natarajan, Kavitha R. Thangaraj, Ashish Goel, C. E. Eapen, K. A. Balasubramanian, Anup Ramachandran
School of Veterinary and Biomedical Sciences: Faculty Publications
Acute fatty liver of pregnancy (AFLP), characterized by hepatic microvesicular steatosis, is a sudden catastrophic illness occurring almost exclusively in the third trimester of pregnancy. Defective fatty acid oxidation in the fetus has been shown to be associated with this disease. Since the placenta has the same genetic makeup as the fetus and as AFLP patients generally recover following delivery, we hypothesized that the placenta might be involved in pathogenesis of this disease. In an animal model of hepatic microvesicular steatosis (using sodium valproate), we found that microvesicular steatosis results in mitochondrial structural alterations and oxidative stress in subcellular organelles …
World Veterinary Year 2011 250 Years Veterinary Education In The World,
2011
Chulalongkorn University
World Veterinary Year 2011 250 Years Veterinary Education In The World, Achariya Sailasuta
The Thai Journal of Veterinary Medicine
No abstract provided.
Investigation Of The Relationship Between Endometrial Mmp Activities And Sex Hormones In Mature Bitches,
2011
Chulalongkorn University
Investigation Of The Relationship Between Endometrial Mmp Activities And Sex Hormones In Mature Bitches, Wei-Yau Shia, Po-Chen Chu, Chih-Chung Chou, Wei-Ming Lee
The Thai Journal of Veterinary Medicine
Endometrial matrix metalloproteinases (MMPs) are extensively studied in humans. However, owing to thedistinct differences of the estrous cycle of bitches, the expression and activities of endometrial MMPs regulated by sexhormones should be significantly different. To understand the effect of sex hormones on the activity of MMPs, thisstudy investigates the activities of endometrial MMP-2 and MMP-9 during different phases of the estrous cycle inbitches through the use of gelatine zymography. Estradiol and progesterone concentrations were also determinedusing enzyme-linked fluorescent assay. The activities of the latent forms of both MMP-2 and -9 were significantlyhigher in metestrus (MP) and late diestrus phases (L-DP) …
Analysis On Differentially Expressed Genes Of Muscle Tissues In Rugao Chicken At The Ages Of 2 And 12 Weeks By Microarray,
2011
Chulalongkorn University
Analysis On Differentially Expressed Genes Of Muscle Tissues In Rugao Chicken At The Ages Of 2 And 12 Weeks By Microarray, Qin De Luan, Guo Bin Chang, Ting Zhen, Zheng Yang Huang, Wei Zhou, Yan Liu, Guo Hong Chen
The Thai Journal of Veterinary Medicine
Microarray containing 13319 probes was used to construct gene expression profiles in order to screendifferentially expressed genes of muscle tissues in Rugao chicken and investigate the molecular mechanism relatedwith muscle tissue traits at 2 and 12 weeks old of chicken. Two hundred and eight differentially expressed genes, ofwhich 108 were known genes, 94 up-regulated, 114 down-regulated were involved in growth, molecular mechanism,fat metabolism, cell proliferation, transcription and splicing factor, protein synthesis and degradation. Meanwhile,some genes of these differentially expressed genes that had no annotation in GenBank were screened out, they werepresumed to be unknown new genes. The differential expressions of …
Seroprevalence And Risk Factors Associated With Caprine Arthritis-Encephalitis Virus Infection In Goats In The Western Part Of Thailand,
2011
Chulalongkorn University
Seroprevalence And Risk Factors Associated With Caprine Arthritis-Encephalitis Virus Infection In Goats In The Western Part Of Thailand, Thant Nyi Lin, Saroch Ngarmkum, Kanisak Oraveerakul, Prachin Virakul, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
To determine the seroprevalence of caprine arthritis-encephalitis virus infection (CAEV) in goats in theWestern part of Thailand, a cross-sectional serological survey was conducted in three provinces, Ratchaburi,Petchaburi and Kanchanaburi, situated in the western part of the country along the border area. A total of 1,129serum samples from 74 randomly selected goat farms containing different breeds of goats were collected during theperiod from November 2009 to January 2011. Seroprevalence of CAEV antibodies was determined using competitiveenzyme-linked immunosorbent assay (cELISA) test. To investigate the risk factors associated with the seroprevalenceof CAEV antibodies, semi-structural questionnaires were developed and presented to farm owners to …
Effects Of Dietary Protein Deficiency On Plasma Amino Acids And Physical – Histological Conformation Of The Mammary Glands In Lactating Sows,
2011
Chulalongkorn University
Effects Of Dietary Protein Deficiency On Plasma Amino Acids And Physical – Histological Conformation Of The Mammary Glands In Lactating Sows, Patrapan Rungcharoen, Sarinee Kalandakanond-Thongsong, Boonrit Thongsong
The Thai Journal of Veterinary Medicine
Eleven multiparous crossbred lactating sows were used to determine the influence of deficient protein dietcontaining 8.2% crude protein and normal protein diet containing 18.2% crude protein on plasma amino acidconcentrations of sows and their piglets, physical mammary gland and histological changes of mammary cells. Theywere provided sufficient water and feed access to one of two isocaloric diets, either deficient (6 sows) or normalprotein diets (5 sows). The experimental period started from farrowing (day 0) until weaning day (day 28). In thisstudy, sows fed deficient protein diet had significant increase in body weight losses and their piglets had significantdecrease in body …
Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo,
2011
Chulalongkorn University
Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan
The Thai Journal of Veterinary Medicine
Virus infection requires the interaction of a virus protein with the host-cell receptor. The Influenza A virusreceptor consists of sialic acid linked to the galactose unit in a α 2,3 or α 2,6 conformation. Various types of thesereceptors are expressed differently on the epithelial lining of various animal species. Here we characterized the typesof receptors that are expressed in the upper and lower respiratory tracts of cow, buffalo, pig and quail. Our findingsdemonstrate that SA α 2,6-gal linked receptors for human influenza viruses are present in the lower respiratory tractof cow and buffalo, while the SA α 2,3-gal linked receptors …
Survey Of Enterococcus Faecalis In Diseased Swine In Hunan Province, China,
2011
Chulalongkorn University
Survey Of Enterococcus Faecalis In Diseased Swine In Hunan Province, China, Wei Jiang, Shi-Cheng Tian, Run-Cheng Li, Yun-Qiu Yan, Ying-Zi Wang, Man-Xiang Li
The Thai Journal of Veterinary Medicine
The prevalence of Enterococcus faecalis was investigated in diseased swine in Hunan Province, China betweenFebruary 2007 and February 2011. A total of 385 diseased swine dissected in prosectorium from 10 representativeadministrative regions in Hunan Province were examined for the presence of E. faecalis. Forty-two out of 385 (10.91%)diseased swine were found to be infected with E. faecalis by bacteria isolations. E. faecalis was mainly recovered fromthe lung and joint cavity. Identification of the organisms was based on biochemical assays and sequencing of thepartial 16S rRNA. The results of the present investigation have implications for the ongoing control of E. faecalisinfections …
Ultrasound Diagnosis,
2011
Chulalongkorn University
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Observation Of Ruminococcus Strains In Captive Asian Elephant (Elephas Maximus),
2011
Chulalongkorn University
Observation Of Ruminococcus Strains In Captive Asian Elephant (Elephas Maximus), Nattawooti Sthitmatee, Pranisa Mahatnirunkul, Patcharaporn Keawmong, Anucha Sirimalaisuwan, Pinich Boontong, Suvichai Rojanasthien, Sumalee Boonmar
The Thai Journal of Veterinary Medicine
Asian elephant is indigenous to many countries including Thailand, but fermenter microorganisms ingastrointestinal tract of the elephant have not fully been investigated. Therefore, this study aimed to observe thecellulolytic bacteria in Genus Ruminococcus in large intestines of captive Asian elephants (Elephas maximus). Fecalsamples were collected from male and female sucklings, young and adult captive Asian elephants. Forty-fourelephants were divided into 3 groups as followed: A) > 18 years old (n=24); B) 2-18 years old (n=17) and C) < 2 yearsold (n=3). The results revealed that there were 214 (42.8%) isolates of R. flavefaciens, 105 (21.0%) isolates of R. bromii, 90(18.0%) isolates of R. obeum, 54 (10.8%) isolates of R. callidus and 37 (7.4%) isolates of R. albus from all fecal samplesexamined. Interestingly, Ruminococcus strains could be isolated from the weaned elephants, but were not found in thesucklings (p<0.05). In conclusion, cellulolytic bacteria in Genus Ruminococcus were isolated from the large intestines ofcaptive Asian elephants. Moreover, the highest prevalence of the bacteria was found in the elephants aged more than18 years old.
The Effect Of Using Long Term And Short Term Extenders During Cooling Process On The Quality Of Frozen Boar Semen,
2011
Chulalongkorn University
The Effect Of Using Long Term And Short Term Extenders During Cooling Process On The Quality Of Frozen Boar Semen, Kampon Kaeoket, Primsiri Chanapai, Puriwat Junchiyaphoom, Panida Chanapiwat
The Thai Journal of Veterinary Medicine
Cooling process of cryopreservation is one of the important factors that affect the qualities of frozen-thawedboar semen. The objective of this study was to compare the qualities of frozen-thawed boar semen after usingdifferent extenders (i.e. BTS, Vitasem LD, ModenaTM and Androstar® plus) during cooling process. Eight sperm-richfractions from 8 boars were employed. The ejaculated semen sample from each boar was divided into four groupsand extended in different freezing extender I as follows: group I (control, short term, BTS), group II (long term,Vitasem LD), group III (long term, ModenaTM) and group IV (long term, Androstar® plus) and kept at 15oC for …
