Veterinary Medicine Commons^™

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

The Virulence Of Thai Isolated Mycoplasma Gallisepticum In Challenged Embryonated Eggs, Somsak Pakpinyo, Suwarak Wanaratana, Sarawoot Mooljuntee

The Thai Journal of Veterinary Medicine

This study was to investigate the virulence and pathogenicity of Thai isolated Mycoplasma gallisepticum (MG)inoculated into embryonated eggs and the chance of virulence study in embryonated eggs instead of experimentalchickens. One hundred and twenty eight-day-old embryonated eggs were divided into 4 groups as follows. Group 1consisting of 12 eggs served as sham negative control and was inoculated with 0.1 ml of broth into yolk sac. Group 2(2.1, 2.2 and 2.3), 3 (3.1, 3.2 and 3.3) and 4 (4.1, 4.2 and 4.3) were inoculated with 0.1 ml of MG strains F, 6/85 and Thaiisolated into yolk sacs, respectively. Each subgroup consisted …

Chronic Toxicity Study Of Garcinia Mangostana Linn. Pericarp Extract, Songpol Chivapat, Pranee Chavalittumrong, Prapai Wongsinkongman, Chada Phisalpong, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

Ethanolic extracts from fruit pericarp of mangosteen (Garcinia mangostana L.) possess many biological andpharmacological activities. However, chronic toxicity study of ethanolic extract has never been investigated. Theobjective of this study was to evaluate the safety of 95% ethanolic extract from mangosteen pericarp in animal model.The oral administration of the extract was performed in 180 Wistar rats randomly allocated to six groups, each of15/sex. Group 1 was control group receiving distilled water. Group 2 to 6 were treatment groups receiving the extractat the doses of 10, 100, 500, 1000 and 1000 mg/kg/day for six months respectively. The last group was assigned …

Emerging Drug-Resistant Superbugs, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

No abstract provided.

Improvement Of Normal Fertilization Rate And Embryo Development By Reduction Of Sperm: Oocyte Ratio During In Vitro Fertilization In Pig, Sasithorn Panasophonkul, Theerawat Tharasanit, Panida Chanapiwat, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The present study was undertaken to determine the influence of sperm:oocyte ratio at fertilization in vitrousing frozen boar semen on fertilization rates and subsequent embryo development and quality in pig. Cumulusoocytecomplexes (COCs) were collected from porcine ovaries and then matured in vitro. After 44 hours of culture,matured oocytes were fertilized for 6 hours with three different sperm:oocyte ratios (1000:1, 2000:1, and 4000:1).Presumptive zygotes were fixed at 18-20 hours post-fertilization and then examined for sperm penetration andmonospermy rates. The developmental competence, in terms of cleavage and blastocyst rates, and the blastocystquality were evaluated at 48 and 168 hours post-fertilization, respectively. Sperm …

Differentiation Potentials Of Canine Bone Marrow Mesenchymal Stem Cells, Theerawat Tharasanit, Nawapen Phutikanit, Chalika Wangdee, Kumpanart Soontornvipart, Sasijaras Tantrajak, Theerayuth Kaewamatawong, Junpen Suwimonteerabutr, Pitt Supaphol, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

Mesenchymal stem cells (MSCs) are multipotent cells that have characteristics of self-renewal anddifferentiation into various specific cell types, in particular mesodermal lineages. This study aimed at isolating,identifying and examining the differentiation capability of canine MSCs. Bone marrow aspirates were obtained from4 dogs. Putative MSCs were then cultured in MSC medium and subpassaged. At the 3rd to 5th passages, MSCs wereexamined for their morphology and doubling time. Two cell lines were examined for the expression of CD 34, CD 44and CD 90, using flow cytometry. The in vitro differentiation of these MSCs into mesodermal lineages (bone, cartilageand adipose tissues) and ectodermal …

Seroprevalence Of Antibodies Against Bartonella Hensalae Infection In Cats And Dogs Along The Northern Borders Of Thailand, Decha Pangjai, Soichi De La Cruz-Hernández, Sumalee Boonma, Wimol Petkanchanapong, Wattanapong Wootta, Pathom Sawanpanyalert

The Thai Journal of Veterinary Medicine

Bartonella hensalae is a causative agent of cat scratch disease that has serious zoonotic potential for people.The aim of the present study is to examine seroprevalence of antibodies against B. hensalae in cats and dogs along thenorthern borders of Thailand in Chiangrai, Maehongson and Nan provinces using the indirect fluorescent antibodytest (IFA). A total of 169 serum samples derived from 56 cats and 113 dogs were collected from 3 provinces along thenorthern borders of Thailand. It was found that the average percentage of positive rate to B. henselae in Chiangrai,Maehongson and Nan provinces was 73.0%, 52.0% and 0.0%, respectively with …

Presence Of Infectious Pancreatic Necrosis Virus On Rainbow Trout (Oncorhynchus Mykiss) By Histopathology, Elisa And Rt-Pcr, Gabriel Aguirre-Guzmán, Ned Iván Iván De La Cruz-Hernández, Jesús Genaro Sánchez-Martínez

The Thai Journal of Veterinary Medicine

Trout (Oncorhynchus mykiss) culture in Mexico experiences sporadic and significant fish mortalities, wheredisease signs are associated with infectious pancreatic necrosis virus disease (IPNV). The purpose of the present workwas to support the potential of ELISA, histopathology, and RT-PCR as routine techniques for IPNV detection.Fourteen trout farms were monitored after a disease outbreak. Positive results were confirmed in different collectedorganisms from 21% of tested farms by all 3 techniques. Virus detection by ELISA and RT-PCR was successfullyperformed in only two days after the initial signs of disease were observed in fish, since these methods are fasterwhen compared to histopathology, which only …

Evaluation Of Immune Responses Following Infection Of Ponies With An Ehv-1 Orf1/2 Deletion Mutant, Gisela Soboll Hussey, Stephen B. Hussey, Bettina Wagner, David W. Horohov, Gerlinde R. Van De Walle, Nikolaus Osterrieder, Lutz S. Goehring, Sangeeta Rao, David P. Lunn

Veterinary Science Faculty Publications

Equine herpesvirus-1 (EHV-1) infection remains a significant problem despite the widespread use of vaccines. The inability to generate a protective immune response to EHV-1 vaccination or infection is thought to be due to immunomodulatory properties of the virus, and the ORF1 and ORF2 gene products have been hypothesized as potential candidates with immunoregulatory properties. A pony infection study was performed to define immune responses to EHV-1, and to determine if an EHV-1 ORF1/2 deletion mutant (ΔORF1/2) would have different disease and immunoregulatory effects compared to wild type EHV-1 (WT). Infection with either virus led to cytokine responses that coincided with …

Caring For Compromised Cattle: Assessing Animals At Risk

Nebraska Beef Quality Assurance Program

Cattlemen have long recognized the need to properly care for their livestock. Sound animal husbandry practices – based on research and decades of practical experience – are known to impact the well-being of cattle, individual animal health and herd productivity. There is no doubt that producers work hard to ensure that their cattle are properly cared for throughout all stages of production. Unfortunately, it’s a fact of raising cattle that some animals will become injured to the extent that they are unable to stand or walk without assistance.

Assessor’S Guide To A Beef Quality Assurance Cow-Calf Assessment

Nebraska Beef Quality Assurance Program

The BQA Cow-Calf Assessment is an on-site educational tool that allows for assessing and benchmarking key indicators of animal care and well-being as well as operational conditions. The Cow-Calf Assessment focuses on three main areas – Animals, Records and Best Management Practices (BMP), and Facilities and Equipment.

Katrina's Animal Legacy: The Pets Act, Marita Mike, Rebecca Mike, Clark J. Lee

Homeland Security Publications

This article discusses issues related to the federal Pets Evacuation and Transportation Standards Act of 2006 (PETS Act), which was signed into law in the aftermath of Hurricane Katrina. Issues discussed in this article include:

1. Various problems concerning animal evacuations and sheltering that Hurricane Katrina brought to light;
2. Provisions of the PETS Act and related laws and policies which were developed in response to the tragedies brought about by Hurricane Katrina; and
3. Strengths and weaknesses of the PETS Act and recommends next steps to improve implementation of the PETS Act.

Growing South Dakota (Winter 2011), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Introducing iGrow: New Web Portal Brings Information To One Location
[Page] 4 Crops, Cattle & Much More: Opportunities Farm Cultivates Research, Teaching And Scholarships
[Page] 7 College News
[Page] 8 Funding Fundamentals: Budget Cuts Have Far-Reaching Impact
[Page] 10 The Value Extension: Three Producers Share How They've Benefited [Page] 12 A Vision for Precision: Gregg Carlson Has Helped Guide Agronomy
[Page] 13 A Boost for Beginning Beef Producers: Training Program Offered Through USDA Grant
[Page] 14 Ethanol's Evolution: Promising New Research Goes Beyond Corn
[Page] 16 New Era for Cereal Grains: Seed Technology Lab Inspires Innovation
[Page] 18 …

Consensus-Based Reporting Standards For Diagnostic Test Accuracy Studies For Paratuberculosis In Ruminants, Ian A. Gardner, Søren S. Nielsen, Richard J. Whittington, Michael T. Collins, Douwe Bakker, Beth Harris, Srinand Sreevatsan, Jason E. Lombard, Raymond Sweeney, David L. Smith, Jerie Gavalchin, Shigetoshi Eda

Zoonotics and Wildlife Disease: Publications

The Standards for Reporting of Diagnostic Accuracy (STARD) statement was developed to encourage complete and transparent reporting of key elements of test accuracy studies in human medicine. The statement was motivated by widespread evidence of bias in test accuracy studies and the finding that incomplete or absent reporting of items in the STARD checklist was associated with overly optimistic estimates of test performance characteristics. Although STARD principles apply broadly, specific guidelines do not exist to account for unique considerations in livestock studies such as herd tests, potential use of experimental challenge studies, a more diverse group of testing purposes and …

Bovine Tuberculosis In A Nebraska Herd Of Farmed Elk And Fallow Deer: A Failure Of The Tuberculin Skin Test And Opportunities For Serodiagnosis, W. Ray Waters, Gary E. Stevens, Mark A. Schoenbaum, Kathy A. Orloski, Suelee Robbe-Austerman, N. Beth Harris, S. Mark Hall, Bruce V. Thomsen, Arach J. Wilson, Roger E. Brannian, Jeffrey T. Nelson, Shawn Schafer, Javan Esfandiari, Meghan Dutton, Rena Greenwald, Konstantin P. Lyashchenko

Zoonotics and Wildlife Disease: Publications

In 2009, Mycobacterium bovis infection was detected in a herd of 60 elk (Cervus elaphus) and 50 fallow deer (Dama dama) in Nebraska, USA. Upon depopulation of the herd, the prevalence of bovine tuberculosis (TB) was estimated at ∼71–75%, based upon histopathology and culture results. Particularly with elk, gross lesions were often severe and extensive. One year ago, the majority of the elk had been tested for TB by single cervical test (SCT), and all were negative. After initial detection of a tuberculous elk in this herd, 42 of the 59 elk were tested by SCT. …

Distribution Of Culicoides Sonorensis (Diptera: Ceratopogonidae) In Nebraska, South Dakota, And North Dakota: Clarifying The Epidemiology Of Bluetongue Disease In The Northern Great Plains Region Of The United States, E. T. Schmidtmann, M. V. Herrero, A. L. Green, D. A. Dargatz, J. M. Rodriquez, T. E. Walton

Zoonotics and Wildlife Disease: Publications

The presence or absence of the biting midge Culicoides sonorensis Wirth & Jones (Diptera: Ceratopogonidae), a primary vector of bluetongue viruses (genus Orbivirus, family Reoviridae, BTV) in North America, was assessed on ranches and farms across the Northern Great Plains region of the United States, specifically Nebraska, South Dakota, and North Dakota, as part of a 2-yr regional study of BTV exposure among cattle. Blacklight/suction trap samples totaling 280 2-night intervals were taken at 140 aquatic sites (potential larval habitat for C. sonorensis) on 82 livestock operations (ranches and farms) that span a south-to-north gradient of expected decreasing …

Effect Of Ejaculation Frequency On Spermatozoa Survival In Diluted Semen From Pleven Blackhead Rams, Stanimir Yotov, Ivan Fasulkov, Nasko Vassilev

Turkish Journal of Veterinary & Animal Sciences

The effect of ejaculation frequency on spermatozoa survival in diluted Pleven Blackhead ram semen was investigated. Thirty-two ejaculates were examined. From each ram 4 ejaculates were collected at 10-min intervals in the morning and in the afternoon. Semen parameters, namely, color, density, transparency, volume, concentration, motility, and abnormal sperm, for each ejaculate were determined. The sperm motility was assessed from the moment of dilution up to 60 min at 10-min intervals, then again at 90 min and 120 min. The semen characteristics were determined by means of "Motic Image Plus" equipment and the data were processed statistically. Sperm volume and …

2011 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

Cow/Calf

• Winter Grazing System and Supplementation of Beef Cows During Late Gestation Influence Heifer Progeny
• Influencing Steer Performance Through Maternal Nutrition
• Integration of DNA Marker Information into Breeding Value Predictions
• Granulosa Cell Gene Expression is Altered in Follicles from Cows with Differing Reproductive Longevity

Growing

• Performance of Growing Cattle Fed Corn Silage or Grazing Corn Residue from Second Generation Insect-protected (MON 89034), Parental, or Reference Corn Hybrids
• Nutrient Composition of Spoiled Wet Byproducts Mixed and Stored with Straw
• Use of Dry Rolled Corn, Dry or Wet distillers Grains Plus Solubles as an Energy Source in High Forage Diets for Growing …

Pituophis Catenifer Deserticola (Great Basin Gophersnake) Predation, Steven J. Wamback, Richard M. Engeman

United States Department of Agriculture Wildlife Services: Staff Publications

A variety of taxa have been observed to· prey upon Pituophis catenifer, including mammals, birds-of prey, and other snakes. Here, we describe an unusual two-day observation of predation on a mature Great Basin gophersnake by a brown trout (Salmo trutta).

The observation took place in Jones Canyon, Utah, USA, on the grounds of the Jones Hole National Fish Hatchery (JHNFH). Jones Creek' emanates from springs at the head of Jones Canyon on hatchery grounds, and flows north to south 6.4 km to the Green River, approximately paralleling the Utah-Colorado border. The stream runs for about 0.3 km …

Roles Of The 15-Kda Selenoprotein (Sep15) In Redox Homeostasis And Cataract Development Revealed By The Analysis Of Sep 15 Knockout Mice, Marina V. Kasaikina, Dmitri E. Fomenko, Vyacheslav M. Labunskyy, Salil A. Lachke, Wenya Qiu, Juliet A. Moncaster, Jie Zhang, Mark W. Wojnarowicz, Sathish Kumar Natarajan, Mikalai I. Malinouski, Ulrich Schweizer, Petra A. Tsuji, Bradley A. Carlson, Richard L. Maas, Marjorie F. Lou, Lee E. Goldstein, Dolph L. Hatfield, Vadim N. Gladyshev

School of Veterinary and Biomedical Sciences: Faculty Publications

The 15-kDa selenoprotein (Sep15) is a thioredoxin-like, endoplasmic reticulum-resident protein involved in the quality control of glycoprotein folding through its interaction with UDP-glucose:glycoprotein glucosyltransferase. Expression of Sep15 is regulated by dietary selenium and the unfolded protein response, but its specific function is not known. In this study, we developed and characterized Sep15 KO mice by targeted removal of exon 2 of the Sep15 gene coding for the cysteinerich UDP-glucose:glycoprotein glucosyltransferase-binding domain. These KO mice synthesized a mutant mRNA, but the shortened protein product could be detected neither in tissues nor in Sep15 KO embryonic fibroblasts. Sep15 KO mice were viable …