Veterinary Medicine Commons^™

Three Rs Approaches In The Production And Quality Control Of Fish Vaccines, Paul J. Midtlyng, Coenraad F.M. Hendriksen, Elisabeth Balks, Lukas Bruckner, Lawrence Elsken, Øystein Evensen, Kjetil Fyrand, Allison Guy, Marlies Halder, Penny Hawkins, Gunn Kisen, Anne Berit Romstad, Kira Salonius, Patrick Smith, Lynne U. Sneddon

Veterinary Science and Medicine Collection

The workshop on Three Rs Approaches in the Production and Quality Control of Fish Vaccines aimed a) to identify animal tests currently stipulated for the production and quality control of fish vaccines and to highlight animal welfare concerns associated with these tests; b) to identify viable options to replace, reduce, and refine animal use for fish vaccine testing; and c) to discuss the way forward and set out how the Three Rs may be implemented without jeopardizing the quality of the vaccines. The workshop participants -- experts from academia, regulatory authorities, a scientific animal welfare organization, and the fish vaccine …

Factors Affecting Space Use Overlap By White-Tailed Deer In An Urban Landscape, W. David Walter, Jeff Beringer, Lonnie P. Hansen, Justin W. Fischer, Joshua J. Millspaugh, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Variation in the size and overlap of space use by white-tailed deer (Odocoileus virginianus) has broad implications for managing deer–human conflicts and disease spread and transmission in urban landscapes. Understanding which factors affect overlap of home range by various segments (i.e., age, sex) of an urban deer population has implications to direct contact between deer on disease epidemiology. We assessed size of home range and overlap of space use using the volume of intersection index (VI) for deer in an urban landscape by sex, age, season, and time of day. We found mean space use was larger for …

Low Levels Of Hydrogen Peroxide Stimulate Corneal Epithelial Cell Adhesion, Migration, And Wound Healing, Qing Pan, Wen-Ya Qiu, Ya-Nan Huo, Yu-Feng Yao, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

PURPOSE. Intracellular reactive oxygen species have been reported to associate with growth factor and integrin signalings in promoting cell adhesion in many cell types. This study is to explore if exogenous H2O2 at low levels can be beneficial to cell adhesion, migration, and wound healing.

METHODS. Primary rabbit corneal epithelial cells treated with 0–70 M H2O2 were tested for viability by MTT assay, adhesion by centrifugation assay, focal contacts of vinculin and F-actin by immunofluorescence, activated Src(pY416), EGF receptor (pY845), vinculin(pY1065), FAK(pY397), and FAK(pY576) by immunoblotting. Cell migration was examined with 0–50 M H2O2 using the scratch wound technique. Corneal …

The Investigation Of The Relations Between Insulin-Liked Growth Factor-I And Body Weight And Between Insulin-Liked Growth Factor-I And Sex In Young Cats, Wei-Yau Shia, Anchana Songkaew, Sasisopa Singhanetr, Chih-Chung Chou, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

High plasma IGF-I concentration has been found in large breed young dogs, but not in young cats. Theobjective of this study was to investigate the relation between the concentration of IGF-I and body weight in bothmale and female cats at young age. In this study, the plasma IGF-I concentrations and body weight in 4 female and 4male cats at 5, 11, 17 and 21 months old were examined. No significant difference in the body weight between thefemale and male kittens (3.1±0.2 kg and 3.2±0.3 kg) at 5 months old was found, but at 11, 17 and 21 months old(5.3±0.2 kg, …

Fas Ligand In Swamp Buffalo Oviduct During Follicular And Luteal Phases, Paisan Tienthai, Pongpisut Chivacharern, Kriengyot Sajjarengpong, Pornchalit Assavacheep

The Thai Journal of Veterinary Medicine

Fas ligand (FasL) and its receptor (Fas) are tumor necrosis factor (TNF) members which are involved in theimmune privileged organs, such as the cornea, testis and placenta, by triggering apoptosis in various cell types. Theappearance of a Fas-FasL system might specify the immune privileged status of the buffalo oviduct wherespermatozoa avoid eradication by female immune cells. The objective of this study was to scrutinize the FasL and Fasproteins by immunohistochemistry, FasL mRNA by RT-PCR and the apoptotic degrees by TUNEL analysis in theuterotubal junction (UTJ), isthmus, ampulla and infundibulum of swamp buffalo oviducts at follicular (n=5) and midluteal(n=5) phases. The …

Clinical Study On The Treatment Of Piroline Against Bovine Mastitis, Jian-Ping Liang, Bao-Cheng Hao, Xue-Hong Wang, Zhi-Ting Guo, Wen-Zhu Guo, Ruo-Feng Shang, Lei Tao, Yu Liu, Zhao-Zhou Li, Lan-Ying Hua, Shu-Yang Wang

The Thai Journal of Veterinary Medicine

The study aims to investigate the efficacy of piroline and antibiotics in the treatment of bovine mastitiscaused by Streptococcus uberis (S. uberis) and Escherichia coli (E. coli) during dry-milk period. 1880 cows in dry-milkperiod were divided into 4 groups and treated with penicillin G, ammonia benzyl penicillin, ceftiofur and piroline,respectively. The efficacy of each medicine in treating mastitis caused by E. coli intramammary infection (E. coli IMI)was followed: 31.2% for penicillin G, 36.9% for ammonia benzyl penicillin, 61.3% for ceftiofur, and 64.4% for Piroline.For those caused by S. uberis intramammary infection (S. uberis IMI), the efficacy of ceftiofur was 90% …

Immunolocalization Of Estrogen Receptor Beta, Androgen Receptor And Ki-67 Protein In Testicular Tissues Of Unilateral Cryptorchidism Boar, Sukanya Manee-In, Pinthira Thiengthiantham, Chanlika Prommapan, Kampon Kaeoket

The Thai Journal of Veterinary Medicine

This study was performed to investigate histological structure, expressions of estrogen receptor beta (ERβ),androgen receptor (AR) and proliferation marker (Ki-67 protein) in scrotal and abdominal testicular tissues ofunilateral cryptorchidism prepubertal boars. Testicular tissues were obtained from 8 unilateral cryptorchidism boars.Immunohistochemical staining for ERβ, AR and Ki-67 protein was performed by avidin-biotin-peroxidase complex(ABC) method. The similar histological structure of both scrotal and abdominal testicular tissues was observed.Immunolocalization of Ki-67 was found in the nuclei of germ cells, interstitial cells and Sertoli cells of both scrotal andabdominal testicular tissues. For ERβ and AR, the immunolocalization was found in germ cells and interstitial …

Differentiation Potentials Of Canine Bone Marrow Mesenchymal Stem Cells, Theerawat Tharasanit, Nawapen Phutikanit, Chalika Wangdee, Kumpanart Soontornvipart, Sasijaras Tantrajak, Theerayuth Kaewamatawong, Junpen Suwimonteerabutr, Pitt Supaphol, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

Mesenchymal stem cells (MSCs) are multipotent cells that have characteristics of self-renewal anddifferentiation into various specific cell types, in particular mesodermal lineages. This study aimed at isolating,identifying and examining the differentiation capability of canine MSCs. Bone marrow aspirates were obtained from4 dogs. Putative MSCs were then cultured in MSC medium and subpassaged. At the 3rd to 5th passages, MSCs wereexamined for their morphology and doubling time. Two cell lines were examined for the expression of CD 34, CD 44and CD 90, using flow cytometry. The in vitro differentiation of these MSCs into mesodermal lineages (bone, cartilageand adipose tissues) and ectodermal …

Presence Of Infectious Pancreatic Necrosis Virus On Rainbow Trout (Oncorhynchus Mykiss) By Histopathology, Elisa And Rt-Pcr, Gabriel Aguirre-Guzmán, Ned Iván Iván De La Cruz-Hernández, Jesús Genaro Sánchez-Martínez

The Thai Journal of Veterinary Medicine

Trout (Oncorhynchus mykiss) culture in Mexico experiences sporadic and significant fish mortalities, wheredisease signs are associated with infectious pancreatic necrosis virus disease (IPNV). The purpose of the present workwas to support the potential of ELISA, histopathology, and RT-PCR as routine techniques for IPNV detection.Fourteen trout farms were monitored after a disease outbreak. Positive results were confirmed in different collectedorganisms from 21% of tested farms by all 3 techniques. Virus detection by ELISA and RT-PCR was successfullyperformed in only two days after the initial signs of disease were observed in fish, since these methods are fasterwhen compared to histopathology, which only …

From Microsurgery To Single Blastomere Biopsy For Es Cell Establishment, Chanchao Lorthongpanich, Chuti Laowtammathron, Rangsun Parnpai

The Thai Journal of Veterinary Medicine

Inner cell mass (ICM) is an important source of embryonic stem (ES) cells. There are several methods thathave been developed to increase the efficiency of ICM cell isolation as well as improve the ES cells derivation rate. Inconventional ICM isolation methods, the methods currently in use destroy trophectoderm cells of blastocyst stageembryos in order to release the ICM cell clumps. These conventional methods are considered embryo destructionmethods as the embryos will be unable to survive after ICM is removed. Recently, an alternative method wasreported using single biopsied blastomeres of earlier stage embryos as a source of ES cells. This novel …

The Activity Of Plasma Matrix Metalloproteinase-9 As A Marker To Reflect The Tissue Level Of Mammary Glands Tumor In Dogs, Wei-Yau Shia, Shih-Ming Liu, Chun-Sheng Lee, Jian-Liang Lin, Chih-Chung Chou, Yumi Yuasa, Shih-Chieh Chang, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

In order to understand the change of MMP-2 and MMP-9 in dogs with mammary gland tumor (MGT), theactivity of MMP-2 and MMP-9 in plasma samples, MGT tissues and near by normal mammary gland tissues wereevaluated by gelatin zymography. The levels of MMP-2 and MMP-9 were significantly higher in tumor tissues thanthose in normal gland tissues (p<0.05). Plasma MMP-9 levels in malignant cases were higher than those in benign(p=0.15) and non-tumor cases (p<0.05). Likewise, plasma MMP-2 levels in malignant MGT cases were higher thannon-tumor (p<0.01) and benign cases (p=0.54). Furthermore, plasma MMP-9 levels were reflected and positivelycorrelated with MMP-9 in tumor tissues (r=0.78, p=0.02), but not MMP-2. Our results indicated that the MMP-2 andMMP-9 levels are associated with tumor malignancy, and plasma MMP-9 activity can become a potential marker bothin follow-up and prognosis of canine MGT patients.

Ecg Quiz, Chollada Buranakarl, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

The Virulence Of Thai Isolated Mycoplasma Gallisepticum In Challenged Embryonated Eggs, Somsak Pakpinyo, Suwarak Wanaratana, Sarawoot Mooljuntee

The Thai Journal of Veterinary Medicine

This study was to investigate the virulence and pathogenicity of Thai isolated Mycoplasma gallisepticum (MG)inoculated into embryonated eggs and the chance of virulence study in embryonated eggs instead of experimentalchickens. One hundred and twenty eight-day-old embryonated eggs were divided into 4 groups as follows. Group 1consisting of 12 eggs served as sham negative control and was inoculated with 0.1 ml of broth into yolk sac. Group 2(2.1, 2.2 and 2.3), 3 (3.1, 3.2 and 3.3) and 4 (4.1, 4.2 and 4.3) were inoculated with 0.1 ml of MG strains F, 6/85 and Thaiisolated into yolk sacs, respectively. Each subgroup consisted …

Chronic Toxicity Study Of Garcinia Mangostana Linn. Pericarp Extract, Songpol Chivapat, Pranee Chavalittumrong, Prapai Wongsinkongman, Chada Phisalpong, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

Ethanolic extracts from fruit pericarp of mangosteen (Garcinia mangostana L.) possess many biological andpharmacological activities. However, chronic toxicity study of ethanolic extract has never been investigated. Theobjective of this study was to evaluate the safety of 95% ethanolic extract from mangosteen pericarp in animal model.The oral administration of the extract was performed in 180 Wistar rats randomly allocated to six groups, each of15/sex. Group 1 was control group receiving distilled water. Group 2 to 6 were treatment groups receiving the extractat the doses of 10, 100, 500, 1000 and 1000 mg/kg/day for six months respectively. The last group was assigned …

Genetic Characterization Of Porcine Epidemic Diarrhea Virus (Pedv) Isolates From Southern Vietnam During 2009-2010 Outbreaks, Do Tien Duy, Tat Toan Nguyen, Suphasawatt Puranaveja, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

Porcine epidemic diarrhea virus (PEDV) spike (S) glyprotein and membrane (M) glycoprotein genes arebelieved to have genetic variation. The heterogeneity in those genomic sequences has been reported and is knownessentially for the diverse PEDV pathogenicity. Eight southern Vietnamese PEDVs collected from severe waterydiarrhea piglets of the recent emerging outbreaks (2009-2010) were sequenced and analyzed. The results revealedhigh nucleotide homology of the partial S gene of the current isolates at 98.9-100% and 99.7-100% identity of the fullM gene among these isolates despite dividing into two subclusters of different provincial origins. It should be notedthat the Vietnamese PEDVs contained high differences on …

Emerging Drug-Resistant Superbugs, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

No abstract provided.

Improvement Of Normal Fertilization Rate And Embryo Development By Reduction Of Sperm: Oocyte Ratio During In Vitro Fertilization In Pig, Sasithorn Panasophonkul, Theerawat Tharasanit, Panida Chanapiwat, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The present study was undertaken to determine the influence of sperm:oocyte ratio at fertilization in vitrousing frozen boar semen on fertilization rates and subsequent embryo development and quality in pig. Cumulusoocytecomplexes (COCs) were collected from porcine ovaries and then matured in vitro. After 44 hours of culture,matured oocytes were fertilized for 6 hours with three different sperm:oocyte ratios (1000:1, 2000:1, and 4000:1).Presumptive zygotes were fixed at 18-20 hours post-fertilization and then examined for sperm penetration andmonospermy rates. The developmental competence, in terms of cleavage and blastocyst rates, and the blastocystquality were evaluated at 48 and 168 hours post-fertilization, respectively. Sperm …

Anticlastogenic Effect Of Asiatic Pennywort And Indian Mulberry Using Rodent Erythrocyte Micronucleus Assay, Piengchai Kupradinun, Anong Tepsuwan, Wannee R. Kusamran

The Thai Journal of Veterinary Medicine

Asiatic pennywort [Centella asiatica (L.), Urban, AP] and Indian mulberry (Morinda citrifolia Linn., IM), theAsian plants, have been previously demonstrated in our laboratory that they could markedly enhance the activities ofsome phase II enzymes, but significantly decreased the activities of phase I enzymes, indicating that they may possesscancer chemopreventive potentials. This study was to investigate the anticlastogenic activity of AP and IM against adirect-acting clastogen, mitomycin C (MMC), and indirect-acting clastogens, cyclophosphamide (CYP) and 7, 12-dimethylbenz(a)anthracene (DMBA). Male mice were fed with AP or IM leaves mixing in modified AIN -76 semipurifieddiet, or IM fruit juice or fruit powder solution …