Jatropha Tanjorensis A Flora Of Southeast Nigeria: Isolation And Characterization Of Naringenin And Validation Of Bio-Enhanced Synergistical Activity Of Α- Tocopherol Toward Clinical Isolates Of Resistant Bacterial,
2022
Nnamdi Azikiwe University, Awka, Anambra State 420007, Nigeria
Jatropha Tanjorensis A Flora Of Southeast Nigeria: Isolation And Characterization Of Naringenin And Validation Of Bio-Enhanced Synergistical Activity Of Α- Tocopherol Toward Clinical Isolates Of Resistant Bacterial, Ikechukwu Kingsley Ijoma, Vincent Ishmael Egbulefu Ajiwe
Makara Journal of Science
Jatropha tanjorensis is among the rich floras of Southeast Nigeria and used by ethnic people to treat infections and manage health conditions. Ethnomedicine has long been employed in the treatment of ailments caused by bacterial pathogens. Studies showed that the incorporation of synthetic α-tocopherol to an antibacterial agent improves its activity. However, knowledge about the antibacterial-enhanced activity of plant-based α-tocopherols, especially those isolated from Jatropha tanjorensis, is limited because of the different bioactivities of synthetic and natural α-tocopherols. To determine the phytochemicals in J. tanjorensis, we carried out the structural elucidation of its leaf extracts. Naringenin and α-tocopherol …
Biowill - Characterising Willow Bark Bio-Actives For Skin Therapies,
2022
Department of Biological and Pharmaceutical Sciences, Munster Technological University, Kerry, Ireland
Biowill - Characterising Willow Bark Bio-Actives For Skin Therapies, Arnold Marisa
ORBioM (Open Research BioSciences Meeting)
Willow bark is considered as a disposable by-product when processing willow for biomass. Willow (Salix) is known to contain high value bioactive compounds which include salicin and its derivatives, and other phytochemicals of interest such as polyphenols and flavonoids. The plant is historically known as the primary source of salicylates to which the well-known drug aspirin is derived from. The work forms part of the Interreg project BioWILL, which is focused on integrated “Zero Waste” biorefinery utilising all fractions of willow feedstock for the production of biochemicals and renewable energy. This project aims to investigate the crude and …
Enhanced Antioxidant, Antiviral, And Anticancer Activities Of The Extract Of Fermented Egyptian Rice Bran Complexed With Hydroxypropyl-Β-Cyclodextrin,
2022
The British University in Egypt
Enhanced Antioxidant, Antiviral, And Anticancer Activities Of The Extract Of Fermented Egyptian Rice Bran Complexed With Hydroxypropyl-Β-Cyclodextrin, Khaled Nematallah, Sherif Ashraf Fahmy, Noha Khalil Mahdy, Hesham El-Askry, Meselhy Ragab Meselhy, Hassan El-Said Azzazy
Pharmacy
Egyptian rice bran was fermented with baker’s yeast, and released phenolics were extracted with aqueous methanol to give fermented rice bran extract (FRBE). The analysis of the FRBE with ultra-performance liquid chromatography–electrospray ionization–tandem mass spectrometry revealed 21 compounds, mainly phenolic acids and flavonoids. The FRBE was then complexed with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) via noncovalent host–guest inclusion complexation using the thin-film hydration technique to improve the hydrophilicity and bioactivity of the FRBE. The formation of the inclusion complex was confirmed using HPLC, 1H NMR, FT-IR, and a phase solubility study. In addition, the biological activities of the complex were investigated. The FRBE/HPβCD …
The Design Of A Pcr-Based Assay To Detect And Isolate The Serine Palmitolytransferase Gene From Environmental Bacteria,
2022
University of Mississippi
The Design Of A Pcr-Based Assay To Detect And Isolate The Serine Palmitolytransferase Gene From Environmental Bacteria, Lana Taylor
Honors Theses
Sphingolipids are a natural class of lipids that function as structural elements of cell membranes and signaling molecules for important cellular activities such as cell growth, differentiation, apoptosis, recognition, and adhesion. These lipids can be found universally in eukaryotic cells as well as some species of bacteria, such as those found in the human gut microbiome and in the environment in soils. Though sphingolipid production is rare in bacteria, both eukaryotic and prokaryotic sphingolipid biosynthesis begin with the condensation of serine and palmitoyl CoA into 3-ketodihydrosphingosine catalyzed by the enzyme serine palmitoyltransferase (SPT). In recent years, several studies have shown …
Cytotoxic Activity Of Non-Specific Lipid Transfer Protein (Nsltp1) And Proteomic Evaluation Of Heparin-Binding Proteins From Ajwain (Trachyspermum Ammi) Seeds,
2022
Chapman University
Cytotoxic Activity Of Non-Specific Lipid Transfer Protein (Nsltp1) And Proteomic Evaluation Of Heparin-Binding Proteins From Ajwain (Trachyspermum Ammi) Seeds, Saud Alshammari
Pharmaceutical Sciences (PhD) Dissertations
Trachyspermum ammi, commonly known as ajwain, is a member of the Apiaceae family. It is a therapeutic herbal spice with diverse pharmacological properties, used in traditional medicine for various ailments. However, all previous studies were conducted using small molecule extracts, leaving the protein’s bioactivity undiscovered. The present study demonstrated the antiproliferative activity of ajwain nsLTP1 in MCF-7, AsPC-1, and MCF10A cell lines and its structural stability in human serum and high-temperature conditions. The antiproliferative activity of ajwain nsLTP1 was evaluated in MCF-7 and AsPC-1 cell lines using an MTT assay. Annexin V-FITC and PI staining were used to detect the …
Integration Of Medicinal Fungi Into The Heber W. Youngken Jr. Medicinal Garden,
2022
University of Rhode Island
Integration Of Medicinal Fungi Into The Heber W. Youngken Jr. Medicinal Garden, Anthony Makashov
Senior Honors Projects
The Heber W. Youngken Jr. Medicinal Garden is a one-of-a-kind resource that houses close to 300 medicinal plants and is filled to the brim with educational opportunities. For the College of Pharmacy, this garden has been the centerpiece of many classes taught here at the University of Rhode Island and has roots as a physical teaching space. While the garden contains a plethora of plant species, medicinal fungi have yet to have been included in the garden, despite their relevance as both sources of pharmaceutical drug discovery and as natural products with inherent physiological benefits themselves. The purpose of my …
Association Of Environmental Pollutants Exposure With Pulmonary Fibrosis: A Mini Review Of Molecular Mechanism Mediated,
2022
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Association Of Environmental Pollutants Exposure With Pulmonary Fibrosis: A Mini Review Of Molecular Mechanism Mediated, Soni Siswanto, Bantari W.K. Wardhani
Pharmaceutical Sciences and Research
Pulmonary fibrosis is a specific form of chronic progressive interstitial lung disease. Deposition of extracellular matrix, mainly collagen, is the pathogenic characteristic of pulmonary fibrosis. Many reports show that environmental pollutants, particularly asbestos, silica, mercury, cadmium, and benzo(a)pyrene, are contributed in the etiology of lung injury and a risk factor in the development of idiopathic pulmonary fibrosis (IPF) in humans. Based on its physicochemical properties, environmental pollutant-induced pulmonary fibrosis can be developed after a particular type or dose of exposure. To date, some studies have focused on variant pollutants that are induced. However, the molecular mechanism of various pollutants to …
Challenge And Development Strategy For Colon-Targeted Drug Delivery System,
2022
Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
Challenge And Development Strategy For Colon-Targeted Drug Delivery System, Raditya Iswandana, Kurnia Sari Setio Putri, Fathia Amalia Putri, Maxius Gunawan, Sekar Arum Larasati
Pharmaceutical Sciences and Research
Colon-Targeted Drug Delivery System (CTDDS) is able to improve local therapeutic effects in the treatment of colon-specific diseases, such as Crohn’s disease, Ulcerative Colitis (UC), and Irritable Bowel Syndrome (IBS). A colon can also be a suitable site for systemic delivery of drugs susceptible to extreme gastric pH, such as peptides and protein therapeutics. The physiological conditions of the gastrointestinal tract and the physicochemical properties of drugs are being considered to develop strategies and approaches to overcome emerging challenges. This review will discuss factors, challenges, strategies, and approaches to developing a colon-targeted drug delivery system.
Antihypertensive And Antioxidant Activity Of Herbal Medicine (Jamu) B On Rats Induced By 10% Fructose,
2022
Department of Pharmacy, Faculty of Health Sciences, Universitas Esa Unggul, Jakarta, Indonesia
Antihypertensive And Antioxidant Activity Of Herbal Medicine (Jamu) B On Rats Induced By 10% Fructose, Aprilita Rina Yanti Eff, Reza Ayu Pertiwi, Hermanus Ehe Hurit, Sri Teguh Rahayu, Yonatan Eden
Pharmaceutical Sciences and Research
Prolonged fructose intake can activate the renin-angiotensin-aldosterone system and enhance oxidative stress conditions, causing hypertension and hyperuricemia. This study aimed to determine the antihypertensive and antioxidant activity of Jamu B on the activity of superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) in the kidneys of Sprague Dawley rats induced by 10% fructose. Thirty rats were divided into six groups. Groups B1-B3 were Jamu groups at a dose of 0.12 g/kg BW (B1), 0.24 g/kg BW (B2), and 0.36 g/kg BW (B3); group C (positive control) was given captopril 0.1 g/kg BW, group D (negative control) was given only 10% …
In Vitro Antibacterial And Antifungal Activities Of Sorindeia Madagascariensis And Albizia Harveyi Stem Barks,
2022
School of Pharmacy, Muhimbili University of Health and Allied Sciences
In Vitro Antibacterial And Antifungal Activities Of Sorindeia Madagascariensis And Albizia Harveyi Stem Barks, Paul Malaba Makoye, Sunday Mrutu, Innocent John Daniel, Mourice Nyangabo Mbunde, Joseph Sempombe, Veronica Mugoyela
Pharmaceutical Sciences and Research
The emerging multi-drug-resistant pathogens urge continuous searches for new antimicrobial agents. This study investigated the in vitro antibacterial and antifungal activities of the stem barks of two plants, Sorindeia madagascariensis and Albizia harveyi. Broth microdilution assay was used to determine the minimum inhibitory concentrations (MICs) of hydroethanolic extracts of the stem barks against selected bacteria and fungi. Both plant extracts exhibited activity against all tested microorganisms and their minimum inhibitory concentrations (MICs) against bacteria and fungi were from 1.67 to 5.00 mg/mL and from 1.67 to 10.00 mg/mL, respectively. This study reports the antibacterial and antifungal activities of the …
Mitragyna Speciosa As A Potential Substitute Therapy In Opioid Dependence: A Case Report,
2022
Clinical Research Centre, Hospital Tuanku Fauziah, Perlis, Ministry of Health Malaysia
Mitragyna Speciosa As A Potential Substitute Therapy In Opioid Dependence: A Case Report, Karniza Khalid, Jason S.K. Wong, Ruzita Jamaluddin
Pharmaceutical Sciences and Research
Preclinical data have consistently suggested the pharmaceutical potential of kratom (Mitragyna speciosa), including as a substitute therapy in opioid dependence. In this case report, we present a case of a man with a long history of kratom use to help him cope with his opiate withdrawals while abstaining from illicit drug use. This is the first published detailed case report highlighting the potential use of kratom to aid opioid dependence encountered in the clinical setting. Our article is also supported by literature review with regards to kratom’s safety profile and pharmacological potential.
Ameliorative Effects Of Minor Cannabinoids Over Hiv-1 Tat-Mediated Visceral Pain,
2022
University of Mississippi
Ameliorative Effects Of Minor Cannabinoids Over Hiv-1 Tat-Mediated Visceral Pain, Charlie Worth
Honors Theses
As the total number of people living with HIV continues to rise across the world, an effective HIV treatment is still sought after. While modern-day advanced therapies exist for mitigating much of the negative effects of HIV, the virus remains evasive and problematic in the central nervous system. Thus, even with treatment, many people living with HIV continue to suffer from a plethora of symptoms. However, a large proportion of HIV-positive patients claim to feel a reduction in those persevering symptoms after cannabis usage. This anecdotal evidence has sparked interest in the efficacy of cannabis constituents for HIV therapy. This …
Discovery Of Sigma-2 Ligands And Prioritization Of Marine Cyanobacteria Extracts For Tnbc Drug Discovery,
2022
Duquesne University
Discovery Of Sigma-2 Ligands And Prioritization Of Marine Cyanobacteria Extracts For Tnbc Drug Discovery, Katelyn Grenell, Andrea L. Rague, Sahar Mofidi, Jane E. Cavanaugh, Kevin J. Tidgewell
Undergraduate Research and Scholarship Symposium
The role of natural products in drug development is well established. In recent years, marine cyanobacteria have been regarded as a major source of biologically active metabolites with chemical and Figure 5 pharmacological diversity. These cyanobacterial natural products serve as a promising source of drug A. leads for the discovery of therapeutic agents used in the treatment of many diseases of interest, such as CNS disorders, pain, and cancer. We have generated a library of 409 fractions from 37 field collected cyanobacterial samples and screened these fractions against a panel of CNS receptors using radiolabeled ligand competitive-binding assays. Upon analysis …
Vitamin D Level Testing In An Urban Midwest Clinic: To Test Or Not To Test?,
2022
Aurora Sinai Medical Center, Advocate Aurora Health
Vitamin D Level Testing In An Urban Midwest Clinic: To Test Or Not To Test?, Daniel Mundt, Marianne Klumph, Kayla Heslin, Wajih Askar
Journal of Patient-Centered Research and Reviews
Vitamin D deficiency (VDD) is significantly higher among urban populations in the U.S. Midwest, with African Americans being disproportionately affected. There is ongoing debate surrounding who and how often individuals should be screened for VDD. This study aimed to understand the prevalence of VDD, associated risk factors, and discrepancies in testing at an urban-based internal medicine residency clinic. Data were retrospectively collected on all adult patients seen by the clinic during 2018 and descriptive statistical analysis performed. Among 3976 total patients (mean age: 53 years), 18% (n = 698) had vitamin D levels analyzed, with deficiency found in 71% of …
A Quantitative Analysis Of The Efficacy Of Various Essential Oils Against The Sars Cov-2 Virus,
2022
Brigham Young University - Provo
A Quantitative Analysis Of The Efficacy Of Various Essential Oils Against The Sars Cov-2 Virus, Elizabeth Wagstaff, Chandrelyn Kraczek, Jack Brandon Lopez
Annual Research Symposium
A poster presentation and abstract for the Roseman Symposium. The project focuses on testing 3 essential oil blends and two disinfectants containing an essential oil blend against SARS CoV-2 in response to the COVID-19 pandemic. The project procedure involves plaque assays, disinfection, and neutralization techniques.
Arkansas Women In Stem Conference Presentation: Investigations Of Salvia Officinalis Secondary Metabolites,
2022
Arkansas Tech University
Arkansas Women In Stem Conference Presentation: Investigations Of Salvia Officinalis Secondary Metabolites, Maria Ines Ines Dow
Arkansas Women in STEM Conference
Salvia officinalis or Sage (The “Salvation Plant”) is known to have various health benefits. Salvia officinalis has been known to treat menstrual cycle pains, gastroenteritis problems, improve liver function, as well as in applications for anticancer remedies and neurodegenerative diseases. This fragrant plant is most often found in the Mediterranean, Southeast Asia, and South America. This research aims to test the raw and vinegar-baked medicinal plant to investigate the change in its chemistry and its biological effect. The plant was purchased as dry leaves and stems. It was then grounded and extracted. The extractions were done via a Soxhlet extraction …
Antimicrobial And Cytotoxic Activities Of Ascidian Species Collected From Egyptian Coasts,
2022
Suez Canal University
Antimicrobial And Cytotoxic Activities Of Ascidian Species Collected From Egyptian Coasts, Amany Metwally, Sarah Ali Shalaby, Tarek Ahmed Temraz, Mohammed Ismail Ahmed
Bulletin of Faculty of Pharmacy Cairo University
Discovering novel drugs for curing cancer and infectious diseases is the prime interest of several research areas now. Marine sessile invertebrates have produced secondary metabolites, a lot of which are utilized as novel pharmaceutical agents with diverse effects whether anti-cancer or anti-microbial. Ascidians are marine sessile animals (phylum: Chordata, class: Ascidiacea). Various anti-cancer, anti-viral, and anti-bacterial components are obtained from different bioactive compounds belonging to ascidian species and are in different stages of clinical trials. In this study, five ascidian species which are Polyclinum constellatum, Botryllus eilatensis, Didemnum sp., Symplegma rubra, and Styela canopus were collected from two coastal sites …
Antidiabetic Activity And In Silico Molecular Docking Of Polyphenols From Ammannia Baccifera L. Subsp. Aegyptiaca (Willd.) Koehne Waste: Structure Elucidation Of Undescribed Acylated Flavonol Diglucoside,
2022
The British University in Egypt
Antidiabetic Activity And In Silico Molecular Docking Of Polyphenols From Ammannia Baccifera L. Subsp. Aegyptiaca (Willd.) Koehne Waste: Structure Elucidation Of Undescribed Acylated Flavonol Diglucoside, Noha Swilam, Mahmoud Nawwar, Rasha Radwan, Eman Mostafa
Pharmacy
Chemical investigation of the aerial parts of Ammania aegyptiaca ethanol extract (AEEE) showed high concentrations of polyphenol and flavonoid content, with notable antioxidant activity. Undescribed acylated diglucoside flavonol myricetin 3-O-β-4C1-(600-O-galloyl glucopyranoside) 7-Oβ-4C1-glucopyranoside (MGGG) was isolated from the aerial parts of AEEE, along with four known polyphenols that had not been characterized previously from AEEE. The inhibitory effects of MGGG, AEEE, and all compounds against α-amylase, pancreatic lipase and β-glucosidase were assessed. In addition, molecular docking was used to determine the inhibition of digestive enzymes, and this confirmed that the MGGG interacted strongly with the active site residues of these enzymes, …
Healing Through Mother Earth,
2022
Hollins University
Healing Through Mother Earth, Taylor A. Russell
Dance (MFA) Theses
This thesis deals with mental health, with a focus on Black women. Historically, Black women are often so compromised, being constant caregivers and helping everyone else, that they forget to help themselves, not having the time and financial means to do so. If we go back in the time of slavery, many Black women were taking care of slave owners' children and suckling the white women’s babies instead of their own. By the time they got home and after diligently caring for other people’s children they were focused on their own children, who they had been away from for hours …
Phytochemical Constituents Of Aquilaria Malaccensis Leaf Extract And Their Anti-Inflammatory Activity Against Lps/Ifn-Γ-Stimulated Raw 264.7 Cell Line,
2022
The British University in Egypt
Phytochemical Constituents Of Aquilaria Malaccensis Leaf Extract And Their Anti-Inflammatory Activity Against Lps/Ifn-Γ-Stimulated Raw 264.7 Cell Line, Eman Ramadan, Manar A. Eissa, Yumi Z. H-Y Hashim, Dina M. El-Kersh
Pharmacy
This study aims to identify the major phytochemical constituents in Aquilaria malaccensis (Thymelaeaceae) ethanolic leaf extract (ALEX-M) and elucidate their ability to suppress nitric oxide (NO) production from a murine macrophage-like cell line (RAW 264.7) stimulated by lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Dichloromethane (DCM) and ethyl acetate (EtOAc) fractions of ALEX-M were subjected to column chromatography. Eight known compounds were isolated for the first time from this species. Compounds were identified using spectroscopic techniques (IR, UV, HRESIMS, and 1D and 2D NMR). Anti-inflammatory activity of both extract and isolated compounds were investigated in vitro. The fractions offered the isolation of …
