Musculoskeletal System Commons^™

Local Anesthesia Toxicity, Nicole Mccleery

Nursing Student Class Projects (Formerly MSN)

Local anesthesia (LA) provides a way to relieve temporary pain in a small part of the body and has been used for over 100 years (Fencl, 2015). LA also prevents the passage of surgical stimuli into the central nervous system (CNS) making a surgical procedure less painful for the patient (Noble, 2015). According to Noble, “altering the passage of stimuli from smaller diameter neurons in a confined area with lower drug dosages is called LA, such as the injection of LA around a surgical incision” (Noble, 2015, p.325). LA can be used in a variety of settings, such as in …

Osteopontin Is Linked With Akt, Foxo1, And Myostatin In Skeletal Muscle Cells, Peter P. Nghiem, Joe N. Kornegay, Kitipong Uaesoontrachoon, Luca Bello, Ying Yin, Akanchha Kesari, Priya Mittal, Scott J. Schatzberg, Gina M. Many, Norman H. Lee, Eric P. Hoffman

All Faculty Scholarship for the College of the Sciences

Introduction: Osteopontin (OPN) polymorphisms are associated with muscle size and modify disease progression in Duchenne muscular dystrophy (DMD). We hypothesized that OPN may share a molecular network with myostatin (MSTN).

Methods: Studies were conducted in the golden retriever (GRMD) and mdx mouse models of DMD. Follow-up in-vitro studies were employed in myogenic cells and the mdx mouse treated with recombinant mouse (rm) or human (Hu) OPN protein.

Results: OPN was increased and MSTN was decreased and levels correlated inversely in GRMD hypertrophied muscle. RM-OPN treatment led to induced AKT1 and FoxO1 phosphorylation, microRNA-486 modulation, and decreased MSTN. An AKT1 inhibitor …

Wine Flavor Perception In A Person With Isolated Congenital Aglossia, Naïve Wine Taster, And Sommelier, Kristin Mahood, Long Wang, Betty Mcmicken, Cheryl Rock

Communication Sciences and Disorders Faculty Articles and Research

Objective: This study aimed to augment previous research that investigated flavor perception in isolated congenital aglossia by a whole food/beverage approach. Isolated congenital aglossia is the rare condition of absence of a tongue at birth without the presence of other symptoms. Previous studies confirmed taste perception in isolated congenital aglossia using single taste solutions including sour, salty, sweet, bitter, and umami.

Methods: The current randomized, double-blinded study age- and sex-matched a naïve wine taster and sommelier to the 46 year-old female with isolated congenital aglossia. A Nose and Palate Survey with 54 variables created based on the Court …

Antimicrobial Hydroxyapatite-Gelatin-Silica Composite Pastes With Tunable Setting Properties, Vuk Uskoković, Shreya Ghosh, Victoria M. Wu

Pharmacy Faculty Articles and Research

Bone grafting is one of the commonest surgical procedures, yet all bone substitutes developed so far suffer from specific weaknesses and the search for a bone graft material with ideal physical and biological properties is still ongoing. Calcium phosphate pastes are the most frequently used synthetic bone grafts, yet they (a) often take an impractically long time to set, (b) release the drug content too fast, and (c) do not form pores large enough to accommodate host cells and foster osseointegration. To make up for these deficiencies, we introduced gelatin and silica to pastes composed of 5–15 nm sized hydroxyapatite …

Differential Requirement For Satellite Cells During Overload-Induced Muscle Hypertrophy In Growing Versus Mature Mice, Kevin A. Murach, Sarah H. White, Yuan Wen, Angel Ho, Esther E. Dupont-Versteegden, John J. Mccarthy, Charlotte A. Peterson

Physical Therapy Faculty Publications

Background: Pax7+ satellite cells are required for skeletal muscle fiber growth during post-natal development in mice. Satellite cell-mediated myonuclear accretion also appears to persist into early adulthood. Given the important role of satellite cells during muscle development, we hypothesized that the necessity of satellite cells for adaptation to an imposed hypertrophic stimulus depends on maturational age.

Methods: Pax7^CreER-R26R^DTA mice were treated for 5 days with vehicle (satellite cell-replete, SC+) or tamoxifen (satellite cell-depleted, SC-) at 2 months (young) and 4 months (mature) of age. Following a 2-week washout, mice were subjected to sham surgery or 10 day …

Neuromuscular Function In Women Postpartum, Rita Deering

Dissertations (1934 -)

Efficient abdominal muscle function is important for functional mobility in men and women, and dysfunction of these muscles has been associated with impaired function such as low back pain. This dissertation explored abdominal muscle function in healthy young men and young women who have never been pregnant (nulligravid). As pregnancy and child birth also impact the tissues of the abdominal wall, this dissertation will also explore abdominal muscle function in postpartum women. This dissertation involved three primary aims. Aim1 compared abdominal muscle function and experimental pain perception in males and nulligravid females. Maximal strength over a range of trunk angles, …

The Effect Of Barefoot Running Using Two Running Styles On Lower Extremity Joint Reaction Forces, Jamie M. Rogers Ep-C

Montview Journal of Research & Scholarship

Running is a popular worldwide activity with many varying biomechanical techniques. Investigating potential differences in joint forces can be beneficial in determining if there is a superior biomechanical running pattern. Previous research compared barefoot and shod running, as well as the kinetic effects of varied running styles. The current study investigated the differences in internal joint reaction forces (JRF) at the hip, knee, and ankle joints during running with two different styles. Ten male and ten female participants who naturally run with a rearfoot strike pattern were included in this study. Each subject ran barefoot on an instrumented treadmill for …

Activin Receptor Type 2a (Acvr2a) Functions Directly In Osteoblasts As A Negative Regulator Of Bone Mass, Brian C. Goh, Vandana Singhal, Angelica J. Herrera, Ryan E. Tomlinson, Soohyun Kim, Marie-Claude Faugere, Emily L. Germain-Lee, Thomas L. Clemens, Se-Jin Lee, Douglas J. Digirolamo

Internal Medicine Faculty Publications

Bone and skeletal muscle mass are highly correlated in mammals, suggesting the existence of common anabolic signaling networks that coordinate the development of these two anatomically adjacent tissues. The activin signaling pathway is an attractive candidate to fulfill such a role. Here, we generated mice with conditional deletion of activin receptor (ACVR) type 2A, ACVR2B, or both, in osteoblasts, to determine the contribution of activin receptor signaling in regulating bone mass. Immunohistochemistry localized ACVR2A and ACVR2B to osteoblasts and osteocytes. Primary osteoblasts expressed activin signaling components, including ACVR2A, ACVR2B, and ACVR1B (ALK4) and demonstrated increased levels of phosphorylated Smad2/3 upon …

The Motor Cortical Representation Of A Muscle Is Not Homogeneous In Brain Connectivity, Jo Armour Smith, Alaa Albishi, Sarine Babikian, Skulpan Asavasopon, Beth E. Fisher, Jason Kutch

Physical Therapy Faculty Articles and Research

Functional connectivity patterns of the motor cortical representational area of single muscles have not been extensively mapped in humans, particularly for the axial musculature. Functional connectivity may provide a neural substrate for adaptation of muscle activity in axial muscles that have both voluntary and postural functions. The purpose of this study was to combine brain stimulation and neuroimaging to both map the cortical representation of the external oblique (EO) in primary motor cortex (M1) and supplementary motor area (SMA), and to establish the resting-state functional connectivity associated with this representation. Motor evoked potentials were elicited from the EO muscle in …

Compartmentalized Camp Responses To Prostaglandin Ep2 Receptor Activation In Human Airway Smooth Muscle Cells, Shailesh R. Agarwal, Kathryn Miyashiro, Htun Latt, Rennolds S. Ostrom, Robert D. Harvey

Pharmacy Faculty Articles and Research

Background and Purpose

Previous studies indicate that prostaglandin EP2 receptors (EP2Rs) selectively couple to adenylyl cyclase type 2 (AC2) in non-lipid raft domains of airway smooth muscle (ASM) cells, where they regulate specific cAMP-dependent responses. The goal of the present study was to identify the cellular microdomains where EP2Rs stimulate cAMP production.

Experimental Approach

FRET-based cAMP biosensors were targeted to different subcellular locations of primary human ASM cells. The Epac2-camps biosensor, which expresses throughout the cell, was used to measure bulk cytoplasmic responses. Epac2-MyrPalm and Epac2-CAAX were used to measure responses associated with lipid raft and non-raft regions of the …

Body Mass Index Associations Between Mother And Offspring From Birth To Age 18: The Fels Longitudinal Study, Stacie S. Swanton, Audrey C. Choh, Miryoung Lee, Lloyd L. Laubach, Jon K. Linderman, Stefan A. Czerwinski, Matthew J. Peterson

Health and Sport Science Faculty Publications

Background: Parental obesity is a known determinant of childhood obesity. Previous research has shown a strong maternal influence on body mass index (BMI) during infancy and early childhood.

Objectives: The purpose of this research was to investigate the BMI associations between mother and offspring from birth to age 18 years.

Methods: Participants were selected from the Fels Longitudinal Study. The current study sample includes 427 (215 mother/son and 212 mother/daughter) mother/child pairs. These pairs are repeatedly measured at multiple age groups in children, resulting in a total of 6,263 (3,215 mother/son, 3,048 mother/daughter) observations for data analysis. Inclusion criteria were …

Efficacy Of Electromyography And The Dead Bug Exercise, Taylor M. Scavo, Rachel C. Cooklin, Erika N. Faria, Madeline L. Johnson, Eric Sternlicht

Student Scholar Symposium Abstracts and Posters

The Dead Bug exercise is performed in physical therapy clinics to restore lumbar spine stability and core strength in patients with lower back pain (LBP). The aim of this study was to evaluate the efficacy of using electromyography (EMG) feedback to enhance proper mechanics during the Dead Bug exercise. Sixteen healthy, college age students volunteered as subjects for the study. Subjects performed the Dead Bug (Fig. 1a.) with and without visual EMG cues and were given instructions on how to execute the exercise. Data was recorded using a BTS FREEEMG Analyzer and signal processed and data analyzed using the BTS …

Effects Of Glucosamine Versus Chondroitin Sulfate In Reducing Joint Space Narrowing In Knee Osteoarthritis, Caroline Horton, Ashani Andrews

Physician Assistant Capstones, 2016 to 2019

Objective: To determine if over the counter supplements, chondroitin sulfate and glucosamine, in combination or alone, are effective in the treatment of joint space narrowing (JSN) in knee osteoarthritis.

Design: Systematic Literature Review

Methods: Database search of PubMed and MEDLINE using the search terms “glucosamine”, “chondroitin sulfate”, “osteoarthritis”, and joint space narrowing. Filters were implemented to include articles that only dealt with human subjects, that were published in 2000 or later, and were full-text. Articles were excluded if they were cohort studies, reviews, non-English papers, or had pain as the only outcome.

Results: Sawitzke et al.primary outcomes revealed no …

Perceptions Of Ncaa Division I Baseball Pitchers On Treatment Modalities For Pitching Arm Health, Thomas Williams

Graduate Theses

Throwing athletes of all sports have similarities, even if the sport itself is very different. Throwing an object creates a great amount of stress on the body, particularly at the shoulder and the elbow. A baseball pitcher should train and compete in all three planes of movement-sagittal, frontal and transverse. Training for throwing athletes has two main goals: to increase throwing power and to reduce throwing injuries. Analyzing training habits of a throwing athlete includes an understanding of the modalities they perform in order to prevent potential injuries from occurring. Baseball pitchers are extremely susceptible to throwing injuries, and must …

Effects Of Ankle Weights On Metabolic Response And Muscle Activity On A Lower Body Positive Pressure Treadmill 2017, Saige Hupman

Master's Theses

Lower body positive pressure (LBPP) treadmills are growing in popularity for rehabilitative use, as the benefits of exercising at partially supported body weight may induce faster recovery. It is unknown if there are certain practices that increase exercise intensity while maintaining positive effects of LBPP. Adding ankle weights when walking or running could increase intensity of rehabilitation programs while maintaining the comfort of supported body weight. PURPOSE: To measure metabolic response (VO2, RER, HR, Caloric expenditure), RPE, and lower limb electromyography (EMG) amplitudes of LBPP treadmill walking and running with and without ankle weights. METHODS: Sixteen participants (Age: 21.94 ± …

The Effect Of Blood Flow Restriction Training With The Quadmill On Peak Isometric Knee Extensor Strength 2017, Brandon Schrom

Master's Theses

Traditional training methodologies that improve muscular strength use loads as low as 75% of a person’s one-repetition maximum and as high as 110% of a person’s one repetition maximum. With these high loads comes a greater risk for injury. Blood flow restriction (BFR) training is a potential solution to this problem. BFR training originated in Japan, where it was called Kaatsu. With this method of training, a trainee ties a tourniquet around the proximal end of a limb to reduce blood flow to and from the limb’s muscles. The purpose of this study was to determine whether three-weeks of BFR …

Imposed Versus Affect-Based Resistance Training Intensities On Adherence, Session Affect, Session Perceived Exertion, And Intrinsic Motivation Measures During A Six-Week Program In Novice Female Lifters 2017, Ryan Joseph Brennan

Master's Theses

The American College of Sports Medicine (ACSM) recommends that individuals perform resistance training exercises at a specific intensity based on external load (percentage of one repetition maximum; %1RM). However, only 29.6% of adults in 2013 reported strength training two or more times per week. Furthermore, individuals lifting at recommended percentages vary in their pleasure and displeasure. Self-selected exercise seems to promote positive affective responses, in part, due to the perceived autonomy. The effects of regulating exercise intensity using affect as opposed to imposed intensities as a means for improved fitness, promoting exercise behavior, and enhancing other psychological outcomes have yet …

A Deeper Look Into The Vastus Medialis: A Stabilizer For The Knee, Connie Nicole Barnes

Undergraduate Honors Capstone Projects

Anterior cruciate ligament tears are one of the most common injuries that occur, especially in female athletes. It requires surgery and months of physical therapy to get these athletes back in playing condition. The angle of women’s hips and lack of strength of inner quadriceps muscles enhances the risk of injuries to the knees. One specific muscle that is known for being a stabilizer of the knee and may help in preventing ACL tears is the vastus medialis. By understanding what exercises activate this muscle the most, conditioning plans and preventative workouts need to be created to help female athletes …

Metformin To Augment Strength Training Effective Response In Seniors (Masters): Study Protocol For A Randomized Controlled Trial, Douglas E. Long, Bailey D. Peck, Jenny L. Martz, S. Craig Tuggle, Heather M. Bush, Gerald Mcgwin, Philip A. Kern, Marcas M. Bamman, Charlotte A. Peterson

Physical Therapy Faculty Publications

Background: Muscle mass and strength are strong determinants of a person’s quality of life and functional independence with advancing age. While resistance training is the most effective intervention to combat age-associated muscle atrophy (sarcopenia), the ability of older adults to increase muscle mass and strength in response to training is blunted and highly variable. Thus, finding novel ways to complement resistance training to improve muscle response and ultimately quality of life among older individuals is critical. The purpose of this study is to determine whether a commonly prescribed medication called metformin can be repurposed to improve the response to resistance …

Toward A Research Agenda For Oncology Physical Therapy, Lucinda Pfalzer, Nicole L. Stout, Shana Harrington, Mary Insana Fisher

Physical Therapy Faculty Publications

Research Round-up: Cancer survivors frequently experience cancer treatment–related functional impairments and disability. While a growing body of evidence supports the effectiveness of rehabilitation interventions for these individuals, there are significant gaps in the existing literature and no interdisciplinary agreement on the priorities for cancer rehabilitation research. In 2016, the Oncology Section revised its strategic plan and set an objective to create an oncology rehabilitation research agenda for the physical therapy (PT) profession. To achieve this goal, the scope of the agenda, a development and review process, and dissemination plan need to be developed.