Plant Pathology Commons^™

Development Of Est-Derived Simple Sequence Repeat (Ssr) Markers In Italian Ryegrass (Lolium Multiflorum Lam.), M. Hirata, Y. Miura, T. Takamizo, M. Fujimori

IGC Proceedings (1977-2023)

Italian ryegrass (IRG, Lolium multiflorum Lam.) is an important grass species used for agricultural purposes in Japan. Linkage maps in IRG have been constructed using molecular markers including RFLPs, AFLPs (Inoue et al., 2003) and genomic SSRs. The SSR marker is a useful tool for genome analysis because it is PCR-based with a relatively high level of polymorphism, co-dominantly expressed. The EST-SSR markers are expected to be more conserved and have a higher rate of transferability than genomic SSR markers (Scott et al., 2000), and the gene functions of ESTs may be predicted. Ikeda et al. (2004) …

Improvement Of Native Perennial Forage Plants For Sustainability Of Mediterranean Farming Systems, F. Lelièvre, Florence Volaire

IGC Proceedings (1977-2023)

The amount of water available to agriculture in the Mediterranean is declining because of increasing population pressure and greater incidence of drought. Therefore, the efficiency of the use of water for agricultural production must be maximized and, in this context, perennial forage species have a number of advantages in comparison to the predominantly-used annuals. They can utilize water throughout the whole year besides being able to halt rangeland degradation, restore soil fertility and enhance forage production, thereby contributing to greater sustainability of rain-fed agricultural systems in the southern European Union and North Africa. Despite these advantages, the small size of …

The Potential For Summer-Dormant Perennial Grasses In Mediterranean And Semi-Arid Pastures, F. Lelièvre, Florence Volaire, P. Chapon, M. Norton

IGC Proceedings (1977-2023)

In rain-fed Mediterranean and semi-arid areas, herbage production of perennial grasses depends on their ability to grow efficiently during the rainy seasons and to persist over the dry summer. A key survival strategy in these harsh conditions is summer dormancy (Volaire, 2002). Within the species Dactylis glomerata L., two cultivars (cvs.), contrasting in this trait, were compared in order to analyse their suitability in terms of yield and survival in these environments.

Future Directions In The Molecular Breeding Of Forage And Turf, G. C. Spangenberg, J. W. Forster, D. Edwards, U. John, A. Mouradov, M. Emmerling, J. Batley, S. Felitti, N. O. I. Cogan, K. F. Smith, M. P. Dobrowolski

IGC Proceedings (1977-2023)

Key points

1. Molecular breeding of forage and turf plants and their endosymbionts has entered the post-genomic era with a large amount of structural genomics information and genomic resources available for key forage and turf species and relevant model systems.
2. A primary future challenge is the conversion of this information into useful functional knowledge for the development of molecular breeding technologies and products that address a range of high impact outcome scenarios in forage and turf.
3. High-throughput approaches for spatial and temporal analysis, from genome to phenome, and the respective data integration in a systems biology context will be critical for …

Crown Rust Resistance In Transgenic Italian Ryegrass (L. Multiflorum) Expressing A Rice Chitinase Gene And Crosses With Cytoplasmic Male Sterile Hybrid Ryegrass, W. Takahashi, M. Fujimori, Y. Miura, T. Komatsu, S. Sugita, A. Arakawa, Y. Nishizawa, H. Sato, Y. Mano, T. Hibi, T. Takamizo

IGC Proceedings (1977-2023)

Italian ryegrass (Lolium multiflorum Lam.) is one of the most important forage grasses in the temperate region. In ryegrasses, crown rust (Puccinia coronata) is the most serious foliar fungal disease and brings about a reduction of herbage yield and loss of palatability to grass-eating domestic animals. In this study, we tried to increase tolerance to the pathogen by introducing a rice chitinase gene using particle bombardment.

Genetic And Molecular Characterization Of Temperate And Tropical Forage Maize Inbred Lines, B. Alarcón-Zúñiga, E. Valadez-Moctezuma, T. Cervantes-Martinez, T. Cervantes-Santana, M. Mendoza‐Rodriguez

IGC Proceedings (1977-2023)

Livestock feeding in the Central highland of Mexico is based on harvest, grazing and annual forage conservation, with forage maize being the most important silage crop (Alarcón, 1995). Even though forage maize is extensively bred in Europe, USA and Asia since the 1900’s, this started in Mexico only in the 1960’s, and little is known about genetic diversity in both agronomic and nutritive value traits. Our breeding program goals are to analyze combining ability of biomass and quality predictors and to study the genetic relationship of inbred lines between lowland tropical and temperate races from Mesa Central, by genetic and …

The Use Of Molecular Markers In Genetic Variability Analysis Of A Collection Of Dactylis Glomerata L., R. Costa, G. Pereira, C. Vicente, M. M. Tavares De Sousa

IGC Proceedings (1977-2023)

Dactylis glomerata L. is one of the three most used perennial grasses in Europe (jointly with Lolium perenne and Festuca arundinacea). The main qualities of Dactylis glomerata are high productivity in pure cultures, high level of proteins and tolerance to drought, cold and shade (Mousset & Chosson, 1986). In the last ten years, techniques that allow direct discrimination at the DNA level have encouraged the study of the genetic variability within cultivated populations as well as identification of the diversity available in germplasm banks. DNA polymorphisms have been shown to be efficient in the identification of genetic variability in …

Molecular Characterization And Tissue Culture Regeneration Ability Of The Usa Arachis Pintoi (Krap. And Greg.) Germplasm Collection, M. A. Carvalho, K. H. Quesenberry, M. Gallo-Meagher

IGC Proceedings (1977-2023)

Arachis pintoi Krap. and Greg. is a herbaceous, perennial legume, exclusively native to Brazil. It is considered a multiple use legume, being grown for forage; ground cover in fruits orchards, forest, and low tillage systems; erosion control; and ornamental purposes. Although several cultivars have been released in different countries, little is known about the genetic diversity of the germplasm stored in world genebanks. Our objective was to characterize and evaluate the genetic diversity of the germplasm of 35 accessions of Arachis pintoi at molecular level using RAPD markers. Concurrently, two tissue culture protocols were evaluated for their organogenesis ability. Further, …

Genetic And Phenotypic Diversity Of Swiss Red Clover Landraces, D. Herrmann, B. Boller, F. Widmer, R. Kölliker

IGC Proceedings (1977-2023)

Mattenklee landraces are persistent and locally adapted Swiss red clover populations. About 100 populations are preserved and may represent a significant yet poorly characterised genetic resource for temperate regions. Genetic characterisation is important in order to improve cultivars, manage genetic resources and to maintain or restore biodiversity. The objectives of this study were to analyse genetic diversity, investigate potential ancestry and to elucidate the congruence of phenotypic and genetic structure of Mattenklee landraces.

Improving The Utilisation Of Germplasm Of Trifolium Spumosum L. By The Development Of A Core Collection Using Ecogeographical And Molecular Techniques, K. Ghamkhar, R. Snowball, S. J. Bennett

IGC Proceedings (1977-2023)

A core collection is a sub-set encompassing more than 70% of the variability of all accessions held in a collection (Brown, 1995). The development of one for Trifolium spumosum (bladder clover) could assist in future development of the cultivar within southern Australia. The aim of this work is to develop a core collection of Trifolium spumosum as a model for other pasture legume species using molecular and ecogeographical data.

Evaluation Of Genetic Diversity In White Clover (Trifolium Repens L.) Through Measurement Of Simple Sequence Repeat (Ssr) Polymorphism, J. George, E. Van Zijll De Jong, T. C. Wilkinson, M. P. Dobrowolski, N. O. I. Cogan, K. F. Smith, J. W. Forster

IGC Proceedings (1977-2023)

White clover (Trifolium repens L.) is a key important temperate pasture legume. Due to the obligate outbreeding nature of white clover, individual genotypes within cultivars are highly genetically heterogeneous. Genetic diversity has been assessed within and between 16 elite cultivars derived from Europe, North and South America, New Zealand and Australia.

Genetic Diversity Among Alfalfa Cultivars Using Ssr Markers, S. Flajoulot, J. Ronfort, P. Baudouin, T. Huguet, P. Barre, Christian Huyghe, B. Julier

IGC Proceedings (1977-2023)

Alfalfa (Medicago sativa) is an autotetraploid, allogamous and heterozygous species. Cultivated varieties are synthetic cultivars, usually obtained through 3 or 4 generations of panmictic reproduction of a set of various numbers of parents. The parents can be clones, half-sib or full-sib families. The breeders apply selection pressure for some agronomic traits, to induce changes in the genetic background. The objective of this study was to investigate the differentiation level among seven cultivars originating from one breeding program, and between these cultivars and the breeding pool, with eight SSR markers.

Random Amplified Polymorphic Dna Analysis In Section Pnigma Of The Genus Bromus L., M. Tuna, O. Barzani, K. P. Vogel, Avi Golan-Goldhirsh

IGC Proceedings (1977-2023)

The section Pnigma consists of about 60 species. It was shown that Eurasian species of the section with the same ploidy level have similar nuclear DNA contents (Tuna et al., 2001). A linear correlation between nuclear DNA content and ploidy level was also found (Tuna et al., 2001). Furthermore, karyotypes of Eurasian species are similar (Tuna et al., 2004a) but differ from the North American species (Tuna et al., 2004b). Yet, the genetic relationship among species within the section is poorly known (Armstrong, 1991). The objective of this study was to assess the phylogenetic relationships among …

Utilization Of Ssr To Distinguish Alfalfa Cultivars, G. R. Bauchan, C. He, Z-L. Xia

IGC Proceedings (1977-2023)

Simple sequence repeat (SSR) or microsatellite markers are co-dominant, abundant and hyper-variable molecular markers from eukaryotic genomes that are being widely used in genetic mapping and phylogenetic studies. Currently, the number of available SSR markers is still very limited for use in alfalfa (Medicago sativa). Thus, this study was conducted to develop SSR from alfalfa genomic libraries and EST and BAC sequence data from M. truncatula for use in distinguishing the nine historically recognized U.S. germplasm sources and eleven fall dormancy check cultivars of alfalfa.

Genetic Diversity In Zoysiagrass Ecotypes Based On Morphological Characteristics And Ssr Markers, M. Hashiguchi, S. Tsuruta, T. Matsuo, M. Ebina, M. Kobayashi, H. Akamine, Ryo Akashi

IGC Proceedings (1977-2023)

Zoysiagrass consists of a number of interfertile species, some of which are important grasses for turfgrass and grazing pasture in Japan. Recently, we developed simple sequence repeats (SSRs) markers from Zoysia japonica “Asagake” genomic DNA by enriched genomic library method (Yamamoto et al., 2002). Here we identify genetic diversity in 38 ecotypes of zoysiagrass (Z. matrella and Z. tenuifolia) from a group of southwest islands of Japan based on morphological characteristics and SSR markers.

Genetic Diversity In Colonial Bentgrass (Agrostis Capillaris L.) Revealed By Ecori/Msei And Psti/Msei Aflp Markers, H. Zhao, S. Bughrara

IGC Proceedings (1977-2023)

Colonial bentgrass species (Agrostis capillaris L.) is a potential genetic resource for the improvement of other bentgrass species (Agrostis spp.) with regard to resistance to environmental stresses and diseases. Transferring resistance from colonial to other bentgrass species is a promising goal in turfgrass breeding programs (Belanger, 2003). Assessment of genetic diversity among accessions of colonial bentgrass species will contribute to eliminate undesirable duplications in the germplasm collection and increase the efficiency of research efforts. It will allow researcher to select diverse resistance genes from different sources to incorporate and pyramid these resistance genes into creeping or other bentgrass …

Databases For Managing Genetic Resources Collections And Mapping Populations Of Forage And Related Species, I. Thomas, H. J. Ougham, D. Peltier

IGC Proceedings (1977-2023)

Effective management of plant material used in crop improvement and underpinning research is greatly facilitated by a properly designed data structure accessible by all those working with the material. At IGER we have developed the Aberystwyth Genetic Resources Information System, AGRIS, for managing genetic resources acquired through collecting trips, seed exchange, breeding and transgenic programmes. Recently this has been complemented by MaPIS, a Mapping Populations Information System, which links with AGRIS and allows for storage and documentation of information about plant mapping populations, including pedigrees, status and physical locations of accessions and individual genotypes. IGER also maintains the European Central …

The Importance Of Exotic Forage Germplasm In Feeding New Zealand's Livestock, J. Lancashire

IGC Proceedings (1977-2023)

A number of recent reports in New Zealand have expressed the view that restricted access to new plant genetic material from overseas is a major risk to the future growth of the primary sector (MAF, 2002; Douglas, 2003). The restrictions outlined in this paper are the result of the Hazardous Substances and New Organisms (HSNO) Act and regulations administered by MAF and the Environmental Risk Management Authority (ERMA) since July 1998. This paper reviews the historical role of exotic forage germplasm in plant improvement in New Zealand, and quantifies the current contribution of recently imported plant material to exports from …

Growth, Nitrogen And Phosphorus Economy In Two Lotus Glaber Mill. Cytotypes Grown Under Contrasting P-Availability, D. H. Cogliatti, L. A. Lett, M. S. Barufaldi, P. Segura, J. A. Cardozo

IGC Proceedings (1977-2023)

Lotus glaber Mill. (lotus) is a forage legume with its origin in Europe which has shown an excellent adaptation to the Depressed Pampas of the Province of Buenos Aires, Argentina. The soils colonized by lotus usually have poor drainage, moderate sodium and low extractable P concentrations. An experiment was performed with the aim of comparing the early growth and economy of phosphorus (P) and nitrogen (N) within two L. glaber cytotypes differing in their ploidy level, a commercial diploid versus an induced autotetraploid population (Barufaldi et al., 2001).

Application Of Molecular Diversity In A Forage Grass Breeding Program, Andrew A. Hopkins, M. C. Saha

IGC Proceedings (1977-2023)

Little or no genotypic information is available for many forage grass populations. The degree of genetic similarity within and among populations greatly influences the choice of breeding strategies and germplasm for developing improved cultivars. Molecular markers have proven effective in classifying genetic diversity of a number of perennial grasses (e.g. Fu et al., 2004; Kubik et al., 2001). We present here an overview of our efforts to integrate molecular diversity data into our breeding program.