Initial Events Associated With Virus Pbcv-1 Infection Of Chlorella Nc64a,
2010
Universitat Darmstadt
Initial Events Associated With Virus Pbcv-1 Infection Of Chlorella Nc64a, Gerhard Thiel, Anna Moroni, David Dunigan, James L. Van Etten
Department of Plant Pathology: Faculty Publications
Chlorella viruses (or chloroviruses) are very large, plaque-forming viruses. The viruses are multilayered structures containing a large double-stranded DNA genome, a lipid bilayered membrane, and an outer icosahedral capsid shell. The viruses replicate in certain isolates of the coccal green alga, Chlorella. Sequence analysis of the 330-kbp genome of Paramecium bursaria Chlorella virus 1 (PBCV-1), the prototype of the virus family Phycodnaviridae, reveals <365 protein-encoding genes and 11 tRNA genes. Products of about 40% of these genes resemble proteins of known function, including many that are unexpected for a virus. Among these is a virus-encoded protein, called Kcv, which forms a functional K+ channel. This chapter focuses on the initial steps in virus infection and provides a plausible role for the function of the viral K+ channel in lowering the turgor pressure of the host. This step appears to be a prerequisite for delivery of the viral genome into the host.
Morphological And Molecular Characterization Of Discocriconemella Inarata, An Endemic Nematode From North American Native Tallgrass Prairies,
2010
University of Nebraska-Lincoln
Morphological And Molecular Characterization Of Discocriconemella Inarata, An Endemic Nematode From North American Native Tallgrass Prairies, Thomas O. Powers, Timothy Harris, Rebecca Higgins, Lisa Sutton, Kirsten S. Powers
Department of Plant Pathology: Faculty Publications
Discocriconemella inarata, a plant parasitic nematode species originally discovered in a virgin tallgrass prairie in northwest Iowa, was re-examined by molecular and morphological analyses of topotype material. This species has never been recorded in cultivated fields and could potentially serve as an indicator for high quality prairie habitats. DNA sequence from a conserved 3’ portion of the 18S ribosomal gene exhibited an identical match between D. inarata topotype specimens and topotype specimens of Mesocriconema xenoplax from Fresno, California. Higher resolution sequence analyses using the internal transcribed spacer 1 (ITS1) and a portion of the mitochondrial gene cytochrome b (cytb) …
Isolation Of The Phycodnavirus Pbcv-1 By Biological Laser Printing,
2010
U.S. Naval Research Laboratory
Isolation Of The Phycodnavirus Pbcv-1 By Biological Laser Printing, Lisa A. Fitzgerald, Peter K. Wu, James R. Gurnon, Justin C. Biffinger, Bradley R. Ringeisen, James L. Van Etten
Department of Plant Pathology: Faculty Publications
The Phycodnaviridae family of viruses is diverse genetically but similar morphologically. These viruses infect eukaryotic algal hosts from both fresh and marine waters, and are an important component of aqueous environments. They play important roles in the dynamics of algal blooms, nutrient cycling, algal community structure, and possibly gene transfer between organisms. As such, it is important to identify new viruses within the Phycodnaviridae family. Biological laser printing (BioLP) was used to isolate single virus particles from solution. BioLP prints droplets containing a single virus particle directly onto a host medium, thereby enabling viruses to be isolated from unmodified samples. …
Deep And Comparative Transcriptome Analysis
Of Rice Plants Infested By The Beet Armyworm (Spodoptera Exigua) And Water Weevil (Lissorhoptrus Oryzophilus),
2010
Ohio State University
Deep And Comparative Transcriptome Analysis Of Rice Plants Infested By The Beet Armyworm (Spodoptera Exigua) And Water Weevil (Lissorhoptrus Oryzophilus), R. C. Venu, M. Sheshu Madhav, M. V. Sreerekha, Kan Nobuta, Yuan Zhang, Peter Carswell, Michael J. Boehm, B. C. Meyers, Kenneth L. Korth, Guo-Liang Wang
Department of Plant Pathology: Faculty Publications
The beet armyworm (Spodoptera exigua) and the rice water weevil (Lissorhoptrus oryzophilus) are two important insect pests in rice production. To identify insect-responsive genes in rice, we performed a deep transcriptome analysis of Nipponbare rice leaves infested with both beet armyworm and water weevil using massively parallel signature sequencing (MPSS). Many antisense, alternative, and novel transcripts were commonly and specifically induced and suppressed in the infested tissue. Key genes involved in the defense metabolic pathways such as salicylic acid and jasmonic acid biosynthesis pathways were up-regulated in the infested leaves. To validate theMPSS results, we analyzed …
