Plant Breeding and Genetics Commons^™

Small Rnas Meet Their Targets: When Methylation Defends Mirnas From Uridylation, Guodong Ren, Xuemei Chen, Bin Yu

Center for Plant Science Innovation: Faculty and Staff Publications

Small RNAs are incorporated into Argonaute protein-containing complexes to guide the silencing of target RNAs in both animals and plants. The abundance of endogenous small RNAs is precisely controlled at multiple levels including transcription, processing and Argonaute loading. In addition to these processes, 3' end modification of small RNAs, the topic of a research area that has rapidly evolved over the last several years, adds another layer of regulation of their abundance, diversity and function. Here, we review our recent understanding of small RNA 3' end methylation and tailing.

Unprecedented Heterogeneity In The Synonymous Substitution Rate Within A Plant Genome, Andan Zhu, Wenhu Guo, Kanika Jain, Jeffrey P. Mower

Center for Plant Science Innovation: Faculty and Staff Publications

The synonymous substitution rate varies widely among species, but it is generally quite stable within a genome due to the absence of strong selective pressures. In plants, plastid genes tend to evolve faster than mitochondrial genes, rate variation among species generally correlates between the mitochondrial and plastid genomes, and few examples of intragenomic rate heterogeneity exist. To study the extent of substitution rate variation between and within plant organellar genomes, we sequenced the complete mitochondrial and plastid genomes from the bugleweed, Ajuga reptans, which was previously shown to exhibit rate heterogeneity for several mitochondrial genes. Substitution rates were accelerated specifically …

Identification Of Rna Silencing Components In Soybean And Sorghum, Xiang Liu, Tao Lu, Yongchao Dou, Bin Yu, Chi Zhang

Center for Plant Science Innovation: Faculty and Staff Publications

Background: RNA silencing is a process triggered by 21–24 small RNAs to repress gene expression. Many organisms including plants use RNA silencing to regulate development and physiology, and to maintain genome stability. Plants possess two classes of small RNAs: microRNAs (miRNAs) and small interfering RNAs (siRNAs). The frameworks of miRNA and siRNA pathways have been established in the model plant, Arabidopsis thaliana (Arabidopsis).

Results: Here we report the identification of putative genes that are required for the generation and function of miRNAs and siRNAs in soybean and sorghum, based on knowledge obtained from Arabidopsis. The gene families, including …

Evaluation Of Constitutive Iron Reductase (Atfro2) Expression On Mineral Accumulation And Distribution In Soybean (Glycine Max. L), Marta W. Vasconcelos, Thomas E. Clemente, Michael A. Grusak

Center for Plant Science Innovation: Faculty and Staff Publications

Iron is an important micronutrient in human and plant nutrition. Adequate iron nutrition during crop production is central for assuring appropriate iron concentrations in the harvestable organs, for human food or animal feed. The whole-plant movement of iron involves several processes, including the reduction of ferric to ferrous iron at several locations throughout the plant, prior to transmembrane trafficking of ferrous iron. In this study, soybean plants that constitutively expressed the AtFRO2 iron reductase gene were analyzed for leaf iron reductase activity, as well as the effect of this transgene's expression on root, leaf, pod wall, and seed mineral concentrations. …

Fusarium Head Blight Resistance And Agronomic Performance In Soft Red Winter Wheat Populations, Daniela Sarti Dvorjak

Theses and Dissertations--Plant and Soil Sciences

Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe [telomorph: Gibberella zeae Schwein.(Petch)], is recognized as one of the most destructive diseases of wheat (Triticum aestivum L. and T. durum L.) and barley (Hordeum vulgare L.) worldwide. Breeding for FHB resistance must be accompanied by selection for desirable agronomic traits. Donor parents with two FHB resistance quantitative trait loci (QTL) Fhb1 (chromosome 3BS) and QFhs.nau-2DL (chromosome 2DL) were crossed to four adapted SRW wheat lines to generate backcross and forward cross progeny. F₂ individuals were genotyped and assigned to 4 different groups according to presence/ absence of …

Seed Guide 2014: Unl Extension Circular 103. Winter Seed Guide -- August 2014, Teshome Regassa, P. Stephen Baenziger, Greg Kruger, Dipak Santra

Department of Agronomy and Horticulture: Faculty Publications

WINTER WHEAT PERFORMANCE

RESULTS AT INDIVIDUAL LOCATIONS

Location Summary for Nebraska Winter Wheat Variety Tests - 2014

Entries and Contact Information

Soil Type, Previous Crop, Fertilizers and Herbicides/Fungicides Applied to Nebraska Winter Wheat Variety Tests -2014

Southeast Dryland Wheat Variety Tests - 2014 Saline, Saunders, and Lancaster Counties & Southeast Dryland Wheat Variety Tests 2010- 2014

South Central Dryland Wheat Variety Test - 2014 Clay County & South Central Dryland Wheat Variety Tests 2010-2014

West Central Dryland Wheat Variety Tests - 2014 Furnas, Red Willow, and Lincoln Counties & West Central Dryland Wheat Variety Tests 2010- 2014

West Dryland Wheat …

College Students’ Perceptions Regarding Sensory Aspects Of Conventionally Produced And Unconventionally Produced Foods: Implications For Marketing To The Millennial Generation, Christina Crowder, Catherine W. Shoulders, K. Jill Rucker

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Consumers vote every day on which products line the shelves of grocery stores, co-ops, and niche markets. Public unrest with regard to the environmental, animal welfare, food purity, and human health impacts of agricultural production practices have led to the rise of unconventionally produced (UP) food products. While the sales of UP foods is increasing, studies regarding the qualities of such products that impact consumer purchases have yielded inconsistent results. This study examined students’ perceptions of sensory aspects of conventionally produced (CP) and UP foods to better understand how sensory aspects impact decisions to purchase. Students reported consistent perceptions regarding …

Testing The Fertility And Allelopathic Abilities Of Arctotheca Prostrata (Salisb.) Britten (Asteraceae, Arctotideae), A South African Plant Species That Has Naturalized In California, Jordy Veit

All Graduate Theses, Dissertations, and Other Capstone Projects

Arctotheca prostrata is a perennial plant species native to South Africa that reproduces vegetatively by long runners. In South Africa it also reproduces sexually, producing fruits with fertile seeds. The species was brought to California to serve as a ground cover but fruits had not been reported. Historically, it has been said to be infertile. This study tested the fertility of A. prostrata in California by crossing plants from California with each other and with plants grown from imported South African seeds. Pollen viability was tested and morphological measures were made on heads, leaves, and pollen. Mean Californian and South …

Implementing Green Roofs On Movie Theaters And Shopping Centers: Business Cases In Profitable Sustainability, Ryan J. Miller

Pomona Senior Theses

This thesis presents the business case for installing green roofs on movie theaters and shopping centers. These businesses can then derive increased profits from the environmental benefits of reduced energy use and increased stormwater retention. After presenting the basic design and benefits of a green roof, the thesis develops stand-alone business plans for a movie theater and shopping center. The author finds that green roofs are a profitable sustainability solution for the commercial enterprise.

Phenotypic Variation In Native North American And Invasive Chinese Populations Of Plantago Virginica, Teresa E. Popp

Electronic Theses and Dissertations

Alien plant species can cause significant economic and biological destruction by invading new environments and outcompeting native species. Through experimental research, the mechanisms by which these species invade can be better understood and applied to their management. Plantago virginica is a perennial herb, native to North America that has recently invaded China. It has been known to reduce crop growth and harbor diseases in the introduced range. A common garden study was conducted at the Georgia Southern University greenhouse, comparing twenty populations of P. virginica from both the native North American and invasive Chinese ranges. I examined if there were …

Registration Of ‘Mattern’ Waxy (Amylose-Free) Winter Wheat, P S. Baenziger, R A. Graybosch, D K. Santra, T Regassa, Y Jin, S Wegulo, G Bai, P S. Amand, X Chen, B Seabourn, F Dowell, R Bowden, D M. Marshall

Teshome H. Regassa

‘Mattern’ (Reg. No. CV-1092, PI 665947) hard winter waxy wheat (Triticum aestivum L.) was developed cooperatively by the USDA–ARS and the Nebraska Agricultural Experiment Station and released in 2012. Mattern has red grain color and waxy (amylose-free) endosperm starch. It was released primarily for its unique end-use quality attributes and for grain yield competitive with currently grown Nebraskaadapted cultivars. The waxy starch is conditioned by the presence of three naturally occurring mutations that eliminate production of the enzyme granule-bound starch synthase. Granule-bound starch synthase synthesizes amylose in typical wheats and other cereal crops. Mattern was tested experimentally as NX04Y2107 and …

The Performance Of Bacterial Phytosensing Transgenic Tobacco Under Field Conditions, Michael Harrison Fethe

Masters Theses

Currently the platforms for wide-area detection of environmental contamination are limited. Therefore, there is interest in developing new platforms, especially for use in crop plants to detect and report the presence of biotic and abiotic stress agents. A biosensor uses a biological organism or substrate to detect the presence of an elicitor (i.e., heavy metal, TNT, or bacteria). The foundational groundwork to create biosensors in transgenic plants exists. The creation of bacterial phytosensing transgenic tobacco containing an orange fluorescent protein (OFP) reporter driven by synthetic pathogen-inducible promoters provides a fluorescent signal when infected with phytopathogens for earlier detection in the …

Molecular And Biochemical Mechanisms Of Pathogenesis In The Maize Foliar Pathogen Cercospora Zeae-Maydis, Winfred-Peck Dorleku

Graduate Theses and Dissertations

GLS is a serious foliar disease of maize, a major staple crop grown commercially in the USA for both human and animal feed production, and increasingly, for ethanol production. The disease is caused by two Cercospora species, C. zeae-maydis and C. zeina, both of which infect maize in the USA and in other parts of the world, with yield losses potentially greater than 50%, depending on local conditions. In culture, C. zeae-maydis produces a phytotoxic, host non-specific perylenequinone, cercosporin, and abscisic acid (ABA), for which there is no known pathological or physiological function in the fungus. Experimental evidence indicates …

Tracking The Cellulolytic Activity Of Clostridium Thermocellum Biofilms, Alexandru Dumitrache, Gideon M. Wolfaardt, David Allen, Steven N. Liss, Lee R. Lynd

Dartmouth Scholarship

Microbial cellulose conversion by Clostridium thermocellum 27405 occurs predominantly through the activity of substrate-adherent bacteria organized in thin, primarily single cell-layered biofilms. The importance of cellulosic surface exposure to microbial hydrolysis has received little attention despite its implied impact on conversion kinetics.

Arabidopsis 56–Amino Acid Serine Palmitoyltransferase- Interacting Proteins Stimulate Sphingolipid Synthesis, Are Essential, And Affect Mycotoxin Sensitivity, Athen N. Kimberlin, Saurav Majumder, Gongshe Han, Ming Chen, Rebecca E. Cahoon, Julie M. Stone, Teresa M. Dunn, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty and Staff Publications

Maintenance of sphingolipid homeostasis is critical for cell growth and programmed cell death (PCD). Serine palmitoyltransferase (SPT), composed of LCB1 and LCB2 subunits, catalyzes the primary regulatory point for sphingolipid synthesis. Small subunits of SPT (ssSPT) that strongly stimulate SPT activity have been identified in mammals, but the role of ssSPT in eukaryotic cells is unclear. Candidate Arabidopsis thaliana ssSPTs, ssSPTa and ssSPTb, were identified and characterized. Expression of these 56–amino acid polypeptides in a Saccharomyces cerevisiae SPT null mutant stimulated SPT activity from the Arabidopsis LCB1/LCB2 heterodimer by >100-fold through physical interaction with LCB1/LCB2. ssSPTa transcripts were …

Nebraska Variety And Hybrid Tests: Spring Seed Guide - 2014, Teshome Regassa, Dipak Santra, Charles A. Shapiro, Greg Kruger, Bruce Anderson

Department of Agronomy and Horticulture: Faculty Publications

Corn Hybrid Tests Summary...........3–6

Growing Degree Days and Precipitation by County Charts...........7

Corn Tables 2013 A–F.............8–11

Test Location Map.......................... 11

Corn Hybrid Tests........................12–16

Soybean Variety Tests Summary.....................17–18

Soybean Tables 2013 A–F......19–21

Soybean Variety Tests............22–23

Sunflower Variety Tests......24–28

Pea Variety Test......29

Proso Millet Variety Test .... 30

Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati

Rajesh Pati

Mango (Mangifera indica L.) is the most important fruit crop of the world. India ranks first among world’s mango producing countries accounting for 57.18 % of the total world mango production of 19.22 million tones. With the development of rDNA technology, plant breeders have access to a large number of genes that can be integrated into the plant genome.

Economic Impact Assessment Of Sorghum, Millet And Other Grains Crsp: Sorghum And Millet Germplasm Development Research, Timothy J. Dalton, Yacob A. Zereyesus

INTSORMIL Scientific Publications

As a result of the offsetting effect of a rise in productivity and decline in harvested area, the world sorghum production (tonnes) has been on a horizontal trend. The combined effect of a decline in area harvested and a rise in yield productivity have resulted in an overall increase in world millet production (tonnes) trend.

During the last three decades, significant numbers of breeding lines, parental stocks, germplasm and cultivars have been released through INTSORMIL/host countries collaboration around the world. Some of the remarkable breeding success stories include the release of the first hybrid sorghum Hageen Dura (HD-1) and Striga …

Dynamic Expression Of Imprinted Genes Associates With Maternally Controlled Nutrient Allocation During Maize Endosperm Development, Mingming Xin, Ruolin Yang, Guosheng Li, Hao Chen, John Laurie, Chuang Ma, Dongfang Wang, Yingyin Yao, Brian A. Larkins, Qixin Sun, Ramin Yadegari, Xiangfeng Wang, Zhongfu Ni

Center for Plant Science Innovation: Faculty and Staff Publications

In angiosperms, the endosperm provides nutrients for embryogenesis and seed germination and is the primary tissue where gene imprinting occurs. To identify the imprintome of early developing maize (Zea mays) endosperm, we performed highthroughput transcriptome sequencing of whole kernels at 0, 3, and 5 d after pollination (DAP) and endosperms at 7, 10, and 15 DAP, using B73 byMo17reciprocal crosses.Weobserved gradually increased expression of paternal transcripts in 3- and 5-DAP kernels. In 7-DAP endosperm, the majority of the genes tested reached a 2:1 maternal versus paternal ratio, suggesting that paternal genes are nearly fully activated by 7 DAP. …

The History Of Trial Gardens, Hallie Schmidt

Horticulture and Crop Science

Essay explores the history of home gardening and seed industry in America and the conditions that led to the development of the All-America Selections.