Plant Breeding and Genetics Commons^™

Sma1, A Homolog Of The Splicing Factor Prp28, Has A Multifaceted Role In Mirna Biogenesis In Arabidopsis, Shengjun Li, Ran Xu, Aixia Li, Kan Liu, Liqing Gu, Mu Li, Hairui Zhang, Yueying Zhang, Shangshang Zhuang, Quanhui Wang, Gang Gao, Na Li, Chi Zhang, Yunhai Li, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEADbox pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences primiRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also …

Generation And Evaluation Of Modified Opaque-2 Popcorn Suggests A Route To Quality Protein Popcorn, Ying Ren, Abou Yobi, Leandra Marshall, Ruthie Angelovici, Oscar Rodriguez, David R. Holding

Center for Plant Science Innovation: Faculty Publications

Introducing traits from dent corn to popcorn is challenging because it is difficult to recover adequate popping characteristics. QPM (Quality Protein Maize) is a dent corn variety carrying the opaque-2 (o2) mutation, specifying increased amounts of normally limiting essential amino acids, and modifier genes which restore the wild type vitreous kernel phenotype. In this study, we introgressed o2 and selected for endosperm modification using vitreousness and high 27-kD gamma zein content. In this way, we recovered high-lysine, fully poppable Quality Protein Popcorn (QPP). BC₂F₄ individuals with vitreous kernels were confirmed to be o2 mutants by …

Molecular Tools Enabling Pennycress (Thlaspi Arvense) As A Model Plant And Oilseed Cash Cover Crop, Michaela Mcginn, Winthrop B. Phippen, Ratan Chopra, Sunil Bansal, Brice A. Jarvis, Mary E. Phippen, Kevin M. Dorn, Maliheh Esfahanian, Tara J. Nazarenus, Edgar B. Cahoon, Timothy P. Durrett, M. David Marks, John C. Sedbrook

Center for Plant Science Innovation: Faculty Publications

Thlapsi arvense L. (pennycress) is being developed as a profitable oilseed cover crop for the winter fallow period throughout the temperate regions of the world, controlling soil erosion and nutrients run-off on otherwise barren farmland. We demonstrate that pennycress can serve as a user-friendly model system akin to Arabidopsis that is well-suited for both laboratory and field experimentation. We sequenced the diploid genome of the spring-type Spring 32-10 inbred line (1C DNA content of 539 Mb; 2n = 14), identifying variation that may explain phenotypic differences with winter-type pennycress, as well as predominantly a one-to-one correspondence with Arabidopsis genes, …

Lycophyte Plastid Genomics: Extreme Variation In Gc, Gene And Intron Content And Multiple Inversions Between A Direct And Inverted Orientation Of The Rrna Repeat, Jeffrey P. Mower, Peng-Fei Ma, Felix Grewe, Alex Taylor, Todd P. Michael, Robert Vanburen, Yin-Long Qiu

Center for Plant Science Innovation: Faculty Publications

Lycophytes are a key group for understanding vascular plant evolution. Lycophyte plastomes are highly distinct, indicating a dynamic evolutionary history, but detailed evaluation is hindered by the limited availability of sequences. Eight diverse plastomes were sequenced to assess variation in structure and functional content across lycophytes. Lycopodiaceae plastomes have remained largely unchanged compared with the common ancestor of land plants, whereas plastome evolution in Isoetes and especially Selaginella is highly dynamic. Selaginella plastomes have the highest GC content and fewest genes and introns of any photosynthetic land plant. Uniquely, the canonical inverted repeat was converted into a direct repeat (DR) …

The Role Of Type Iii Effectors From Xanthomonas Axonopodis Pv. Manihotis In Virulence And Suppression Of Plant Immunity, Cesar Augusto Medina, Paola Andrea Reyes, Cesar Augusto Trujillo, Juan Luis Gonzalez, David Alejandro Bejarano, Nathaly Andrea Montenegro, Jonathan M. Jacobs, Anna Joe, Silvia Restrepo, James R. Alfano, Adriana Bernal

Center for Plant Science Innovation: Faculty Publications

Xanthomonas axonopodis pv. manihotis (Xam) causes cassava bacterial blight, the most important bacterial disease of cassava. Xam, like other Xanthomonas species, requires type III effectors (T3Es) for maximal virulence. Xam strain CIO151 possesses 17 predicted T3Es belonging to the Xanthomonas outer protein (Xop) class. This work aimed to characterize nine Xop effectors present in Xam CIO151 for their role in virulence and modulation of plant immunity. Our findings demonstrate the importance of XopZ, XopX, XopAO1 and AvrBs2 for full virulence, as well as a redundant function in virulence between XopN and XopQ in susceptible cassava plants. We tested their role …

Xanthomonas Oryzae Pv. Oryzae Tale Proteins Recruit Ostfiiaγ1 To Compensate For The Absence Of Ostfiiaγ5 In Bacterial Blight In Rice, Wenxiu Ma, Lifang Zou, Zhiyuan Ji, Xiameng Xu, Zhengyin Xu, Yangyang Yang, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight (BB) of rice, uses transcription activator-like effectors (TALEs) to interact with the basal transcription factor gamma subunit OsTFIIAγ5 (Xa5) and activates the transcription of host genes. However, how OsTFIIAγ1, the other OsTFIIAγ protein, functions in the presence of TALEs remains unclear. In this study, we show that OsTFIIAγ1 plays a compensatory role in the absence of Xa5. The expression of OsTFIIAγ1, which is activated by TALE PthXo7, increases the expression of host genes targeted by avirulent and virulent TALEs. Defective OsTFIIAγ1 rice lines show reduced expression of the TALE-targeted …

Genetic Component Effects Of Two Loci On Seed Dormancy, Awn, Low-Temperature Germination, Plant Height, And Flowering Time In Rice (Oryza Sativa L.), Ugur Korkmaz

Electronic Theses and Dissertations

Seed dormancy (SD) is an adaptive trait of both ecological and agricultural importance. Cereal crops have been selected for reduced SD to promote germination, which also caused the pre-harvest sprouting (PHS) problem in crop production. The previous research identified a set of quantitative trait loci (QTL) for SD, including qSD1- 2 and qSD8, in the conspecific weedy and cultivated rice (Oryza sativa). qSD1-2 carries a dormancy-enhancing allele from the cultivated rice and has a pleiotropic effect on plant height. qSD8 carries a dormancy-enhancing allele from the weedy rice and tightly links to qAL8 for awn length. The …

Exploiting The Genetic Diversity Of Wild Ancestors And Relatives Of Wheat For Its Improvement, Jagdeep Singh Sidhu

Electronic Theses and Dissertations

Wheat is the third most staple food worldwide but current 1% annual improvement in the wheat production is insufficient to meet the growing demands in future. The narrow genetic base of wheat limits continuous improvement in wheat productivity and tolerance to biotic and abiotic stresses under changing climate. Wild ancestors and relatives of wheat hold a potential in widening the genetic pool of wheat and enhance its resilience to biotic and abiotic stresses. This study was focused towards characterizing the genetic diversity in wild relatives of wheat for disease resistance and efficient association with diazotrophs. In the first study, we …

Mac3a And Mac3b, Two Core Subunits Of The Mos4-Associated Complex, Positively Influence Mirna Biogenesis, Shengjun Li, Kan Liu, Bangjun Zhou, Mu Li, Shuxin Zhang, Lirong Zeng, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MAC3A and MAC3B are conserved U-box containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis. However, their functional mechanisms remain elusive. Here we show that Arabidopsis thaliana MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs ( …

Map-Based Cloning And Molecular Characterization Of The Seed Dormancy 10 Locus In Rice (Oryza Sativa L.), Wirat Pipatpongpinyo

Electronic Theses and Dissertations

Seed dormancy is a key adaptive trait of both ecological and agricultural importance. Although many quantitative trait loci (QTL) have been associated with seed dormancy in cereal crops or model plants, a majority of them remain unknown for molecular identities and functions. Cereal crops and wild/weedy relatives usually have weak and strong seed dormancy, respectively, such as Asian cultivated rice vs. weedy rice (Oryza sativa L.). Previous research identified a set of seed dormancy QTL, including qSD10, in single plant-derived BC₁F₂ and BC₁F₃ populations from the backcross between the weedy rice line SS18-2 …

Identification Of Qtl Modifying The Activity Of The Tcb1-S Locus And Characterization And Sequencing Of Two Plutonium-Beryllium Induced Reduced Gametophyte Transmission Mutants In Maize, Merritt Bryer Burch

Electronic Theses and Dissertations

This thesis is split into two independent projects both involving the male gametophyte generation of maize. The first project looks at how pollen interacts with the female gametophyte to reduce its transmission in cross-incompatible reactions controlled by the unilateral cross-incompatibility system, teosinte crossing barrier 1. The second project explores two plutonium-beryllium induced male gametophyte mutants and attempts to uncover their genetic basis.

Identification of QTL Modifying the Activity of the Tcb1-s Locus
Teosinte crossing barrier 1 (Tcb1) is a unilateral cross-incompatibility system present in maize that provides a pre-zygotic pistil barrier to plants carrying Tcb1-s (strong allele) from pollen …

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

This paper reports on six bicolor sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

This paper reports on fifteen bicolor, two yellow, and two white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Midwest Vegetable Trial Report For 2017, Elizabeth Maynard, Brad R. Bergefurd, Wenjing Guan, Petrus Langenhoven

Midwest Vegetable Trial Reports

This is a compilation of 19 research trial reports from six land-grant universities in the Midwestern United States. Crops include green beans, cantaloupe, cucumber, ornamental corn, pepper, potato, pumpkin, butternut squash, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties. One report addressed specific growing practices: plant spacing for sweet corn.

Intraspecific Genetic Variation, Population Structure, And Performance Of The Invasive Aquatic Macrophyte Eurasian Watermilfoil (Myriophyllum Spicatum) In Waterbodies With And Without Histories Of Chemical Herbicide Treatment Across Michigan, Taylor Zallek

Dissertations, Master's Theses and Master's Reports

Populations of invasive species are often subjected to novel selective forces in the form of anthropogenic control agents in their introduced ranges. These control agents, applied unevenly among populations within a species’ new range, can send invasive populations on drastically different evolutionary and ecological trajectories. In these studies, we aimed to see if different histories of chemical herbicide treatment are differentially influencing the genetic diversity, structure, and performance of populations of invasive watermilfoil (Myriophyllum spicatum and M. spicatum x M. sibiricum hybrids) from waterbodies in the state of Michigan. We sampled ten waterbodies with different histories of herbicide treatment …

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

This paper reports on six bicolor sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

This paper reports on fifteen bicolor, two yellow, and two white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Midwest Vegetable Trial Report For 2017, Elizabeth Maynard, Brad R. Bergefurd, Wenjing Guan, Petrus Langenhoven

Purdue Fruit and Vegetable Research Reports

This is a compilation of 19 research trial reports from six land-grant universities in the Midwestern United States. Crops include green beans, cantaloupe, cucumber, ornamental corn, pepper, potato, pumpkin, butternut squash, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties. One report addressed specific growing practices: plant spacing for sweet corn.

Tomato Cultivar Evaluation In High Tunnels, Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

Tomatoes are a common crop grown in unheated greenhouses, commonly called high tunnels. Production in these structures has increased in recent years. This trial was conducted to evaluate varieties for their performance as a summer-harvested crop in soil-based high tunnel production systems. Five hybrid determinate varieties or line, one hybrid indeterminate, and one open-pollinated indeterminate variety were compared for yield, earliness, fruit size, and quality.

Tomato Cultivar Evaluation In High Tunnels, Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

Tomatoes are a common crop grown in unheated greenhouses, commonly called high tunnels. Production in these structures has increased in recent years. This trial was conducted to evaluate varieties for their performance as a summer-harvested crop in soil-based high tunnel production systems. Five hybrid determinate varieties or line, one hybrid indeterminate, and one open-pollinated indeterminate variety were compared for yield, earliness, fruit size, and quality.