Plant Breeding and Genetics Commons^™

Pumpkin Variety Performance With And Without Treatment For Powdery Mildew In Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

Pumpkins are grown on 5,000 acres in Indiana with an average yield of 6.75 tons per acre. Successful pumpkin production requires the use of varieties that yield well and produce pumpkins of the size, shape, color, and quality demanded by the market. Genetic resistance to the fungal disease powdery mildew is present in some varieties. This trial was designed to evaluate performance of pumpkin varieties in northern Indiana with and without treatment for powdery mildew. The trial included fourteen orange jack-o-lantern size pumpkins, one small or 'pie' pumpkin and one specialty yellow pumpkin.

Pumpkin Variety Performance With And Without Treatment For Powdery Mildew In Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

Pumpkins are grown on 5,000 acres in Indiana with an average yield of 6.75 tons per acre. Successful pumpkin production requires the use of varieties that yield well and produce pumpkins of the size, shape, color, and quality demanded by the market. Genetic resistance to the fungal disease powdery mildew is present in some varieties. This trial was designed to evaluate performance of pumpkin varieties in northern Indiana with and without treatment for powdery mildew. The trial included fourteen orange jack-o-lantern size pumpkins, one small or 'pie' pumpkin and one specialty yellow pumpkin.

Low- Copy Nuclear Markers In Isoëtes (Isoëtaceae) Identified With Transcriptomes, Peter W. Schafran, Gabriel Johnson, W. Carl Taylor, Elizabeth A. Zimmer, Lytton J. Musselman

Biological Sciences Faculty Publications

PREMISE OF THE STUDY: Few genetic markers provide phylogenetic information in closely related species of Isoëtes (Isoëtaceae). We describe the development of primers for several putative low- copy nuclear markers to resolve the phylogeny of Isoëtes, particularly in the southeastern United States.

METHODS AND RESULTS: We identified regions of interest in Isoëtes transcriptomes based on low- copy genes in other plants. Primers were designed for these regions and tested with 16 taxa of Isoëtes and one species of Lycopodium. Parts of the pgiC, gapC, and IBR3 gene regions show phylogenetic signal within the North American and …

An Efficient Protocol For Root Studies In The Common Sunflower Using Composite Plants, Tyler Parks

Masters Theses

Sunflower, Helianthus annuus, is one of the most important oilseed crop in the world. Oil from the seeds is prized for its' exceptional quality and flavor. Despite this, sunflowers have been forced onto marginal lands often on semi-arid and non-fertile soil, due to disease pressures and economics making it critical to produce more resilient lines and varieties to withstand these new stresses. The recent availability of the sunflower genome can allow genome-wide characterization of gene families. Stable transformation protocols, which can be used for characterization studies, have been developed for H. annuus using Agrobacterium tumefaciens, but they produce …

Investigation Of A Putative Membrane-Bound Protein In Arabidopsis Thaliana And Nicotiana Tabacum, Allen Potter

Masters Theses

The NLG (Novel Leaf Gene) is a recently discovered gene from aspen (Populus tremula x alba). Previous research indicates that NLG impacts leaf shape. Our preliminary analyses indicate that NLG may function as a membrane bound protein with possible localization in plasma membrane and/or nucleus. Sequence and phylogenetic analyses in Arabidopsis thaliana and Populus trichocarpa identified four homologous genes in Arabidopsis and five in Populus. Gene expression analysis was done using online tools and indicated that NLG is expressed in multiple tissue types but predominately in stem and flowers. The protein coding region of the NLG orthologs genes from aspen …

Quantitative And Molecular Analysis Of Habituation At The Maize R1 Locus, Robert C. Lindsay

Theses and Dissertations

Epigenetics is the study of heritable changes in phenotypes that are not the result of changes in DNA sequence. Examples of epigenetic affecters include methylation changes, chromatin modifications, transcription factors, and RNA-based changes. The molecular mechanisms behind epigenetic changes are not fully understood. Canalization is the buffering of gene expression against environmental changes over time, while habituation is semi-stable expression change over time due to selection. This work characterized the molecular changes associated with the kernel color changes of the R-sc:86-17pale allele at the maize red color1 (r1) locus to determine if the changes are epigenetic in nature. …

Natural And Anthropogenic Drivers Of Tree Evolutionary Dynamics, Brandon M. Lind

Theses and Dissertations

Species of trees inhabit diverse and heterogeneous environments, and often play important ecological roles in such communities. As a result of their vast ecological breadth, trees have become adapted to various environmental pressures. In this dissertation I examine various environmental factors that drive evolutionary dynamics in threePinusspecies in California and Nevada, USA. In chapter two, I assess the role of management influence of thinning, fire, and their interaction on fine-scale gene flow within fire-suppressed populations of Pinus lambertiana, a historically dominant and ecologically important member of mixed-conifer forests of the Sierra Nevada, California. Here, I find evidence …

Morphological, Genetic And Biochemical Characterization Of The Anti-Malarial Herb, Artemisia Annua Germplasm Collection At West Virginia University, Delini K. Samarasinghe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Malaria is one of the deadliest diseases in human history. Nearly half of the world’s population, is at the risk in 106 countries. Only in 2016, this disease killed about 445,000 people, 72% of them being children under age five. It also accounts for US $12 billion dollars of direct costs in Africa alone. Five different species of Plasmodium cause malaria but P. falciparum is the most detrimental one, causing 50% of all malaria cases and is considered as the deadliest parasite in humans. Artemisinin (ART), a 15 C sesquiterpenoid is currently the only precursor to the most effective anti-malarial …

Provitamin A Biofortification Of Cassava Enhances Shelf Life But Reduces Dry Matter Content Of Storage Roots Due To Altered Carbon Partitioning Into Starch, Getu Beyene, Felix R. Solomon, Raj D. Chauhan, Eliana Gaitán-Solis, Narayanan Narayanan, Jackson Gehan, Dimuth Siritunga, Robin L. Stevens, John Jifon, Joyce Van Eck, Edward Linsler, Malia Gehan, Muhammad Ilyas, Martin Fregene, Richard T. Sayre, Paul Anderson, Nigel Taylor, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Storage roots of cassava (Manihot esculenta Crantz), a major subsistence crop of sub-Saharan Africa, are calorie rich but deficient in essential micronutrients, including provitamin A β-carotene. In this study, β-carotene concentrations in cassava storage roots were enhanced by coexpression of transgenes for deoxy-d-xylulose-5-phosphate synthase (DXS) and bacterial phytoene synthase (crtB), mediated by the patatin-type 1 promoter. Storage roots harvested from field-grown plants accumulated carotenoids to ≤50 lg/g DW, 15- to 20-fold increases relative to roots from nontransgenic plants. Approximately 85%–90% of these carotenoids accumulated as all-trans-β-carotene, the most nutritionally efficacious carotenoid. β-Carotene-accumulating storage roots displayed delayed onset of postharvest …

Pengaruh Pola Tanam Tumpangsari Jagung Dan Kedelai Terhadap Pertumbuhan Dan Hasil Jagung Hibrida Dan Evaluasi Tumpangsari Di Arjasari Kabupaten Bandung, Yuyun Yuwariah, Dedi Ruswandi, Aep Wawan Irwan

Jurnal Kultivasi

Jagung dan kedelai merupakan tanaman pangan terpenting setelah padi di Indonesia. Konsumsi jagung dan kedelai akan terus mengalami peningkatan setiap tahun dikarenakan pertambahan jumlah penduduk. Salah satu upaya untuk meningkatkan produksi tanaman dapat dilakukan dengan cara tumpangsari. Sistem tumpangsari merupakan sistem pertanaman dengan menanam dua atau lebih jenis tanaman secara serentak pada lahan yang sama dalam waktu satu tahun. Penelitian bertujuan untuk mengetahui genotip jagung hibrida yang terbaik ditumpangsarikan dengan kedelai. Percobaan dilaksanakan dari bulan Maret 2016 sampai bulan Agustus 2016 di Arjasari, Kabupaten Bandung dengan ketinggian tempat mencapai 960 m di atas permukaan laut. Rancangan yang digunakan dalam penelitian …

Identifikasi Keragaman Genetik Plasma Nutfah Ubi Kayu Liar (Manihot Glaziovii Muell) Berdasarkan Karakter Morfo-Agronomi, Agung Karuniawan, Hendi N. Wicaksono, Debby Ustari, Tia Setiawati, T Supriatun

Jurnal Kultivasi

Plasma nutfah tanaman merupakan sumber bahan genetik bagi program pemuliaan tanaman. Penelitian ini bertujuan untuk mengidentifikasi keragaman genetik plasma nutfah ubi kayu liar berdasarkan karakter morpho-agronomy. Percobaan dilaksanakan pada bulan Desember 2014 hingga Juni 2015. Rancangan percobaan menggunakan Rancangan Acak Kelompok (RAK) dengan perlakuan 23 aksesi ubi kayu liar dan diulang sebanyak dua kali. Analisis komponen utama menghasilkan biplot dengan nilai PC1 dan PC2 yang memiliki kontribusi terbesar terhadap variasi yaitu masing-masing sebesar 27.98% dan 19.45%. Analisis klaster menghasilkan gambar dendogram terdiri dari dua klaster utama yaitu I dan II. Pada gambar dendogram terdapat jarak euclidean antara 0.00 hingga 3.32. …

Development And Characterization Of Rice Genotypes For Water Use Efficiency And Drought Resistance, Anuj Kumar

Graduate Theses and Dissertations

Rice, the second largest staple food crop, uses 30% of global fresh water to complete its life cycle worldwide. Water deficits worldwide have become a serious problem affecting rice growth and ultimately grain yield. To solve the water problem globally, improvement of water use efficiency (WUE) and other drought resistance (DR) traits in rice genotypes would be a useful strategy using advanced genomics tools. In this study, our objectives were to 1) analyze phenotypes of the USDA rice mini-core collection (URMC) for WUE and DR related traits, 2) correlate drought response phenes for physiological traits and grain yield in the …

Development And Evaluation Of Environmentally-Sensitive Genetic Male Sterile Lines For Louisiana Hybrid Rice, Federico Molina Casella

LSU Doctoral Dissertations

The Louisiana rice industry generates ~ 400 million dollars each year toward our state economy. Louisiana varieties are typically bred as pure lines, but hybrid (F₁) varieties have the potential to increase grain yields 15% or more over conventional sources. A primary goal of the LSU AgCenter Hybrid Breeding program is to breed elite cross combinations (hybrid varieties) through development of environmentally sensitive male sterile and fertile pollinator lines. Extensive multi-location yield trials in Louisiana have demonstrated high grain and head rice yields for candidate hybrids, but improvements in maturity, lodging, and grain chalk are needed. To address …

Inheritance Study Of Resistance To The Herbicide Quizalofop-P-Ethyl In Rice And Development Of Provisia Hybrid Rice Germplasm, Jose Roberto Camacho Montero

LSU Doctoral Dissertations

In 2017, Provisia^TM quizalofop-p-ethyl (QFE) resistant rice became a new alternative for the chemical control of in-season red rice (Oryza sativa L. sp. spontaneum) infestations in Louisiana rice farms. Until then, Clearfield^® imidazolinone resistant rice was the only alternative that US farmers had for the efficient in-season chemical control of red rice. Provisia^TM rice is a BASF Corporation patented technology that can serve as an alternative for rotation with Clearfield^® rice. This strategy seeks to prevent outcrosses between red rice and herbicide resistant varieties, and consequently extend the life span of both technologies. This …

Loci On Chromosomes 1a And 2a Affect Resistance To Tan (Yellow) Spot In Wheat Populations Not Segregating For Tsn1, Manisha Shankar, Dorthe Jorgensen, Julian Taylor, Ken J. Chalmers, Rebecca Fox, Grant J. Hollaway, Stephen M. Neate, Mark S. Mclean, Elysia Vassos, Hossein Golzar, Rob Loughman, Diane E. Mather

Biosecurity Research Articles

Tan spot (syn. yellow spot and yellow leaf spot) caused by Pyrenophora tritici-repentis is an important foliar disease of wheat in Australia. Few resistance genes have been mapped in Australian germplasm and only one, known as tsn1 located on chromosome 5B, is known in Australian breeding programs. This gene confers insensitivity to the fungal effector ToxA. The main aim of this study was to map novel resistance loci in two populations: Calingiri/Wyalkatchem, which is fixed for the ToxA-insensitivity allele tsn1, and IGW2574/Annuello, which is fixed for the ToxA-sensitivity allele Tsn1. A second aim was to combine …

First Interspecific Genetic Linkage Map For Castanea Sativa X Castanea Crenata Revealed Qtls For Resistance To Phytophthora Cinnamomi, Carmen Santos, Charles Dana Nelson, Tetyana Zhebentyayeva, Helena Machado, José Gomes-Laranjo, Rita Lourenço Costa

Forest Health Research and Education Center Faculty Publications

The Japanese chestnut (Castanea crenata) carries resistance to Phytophthora cinnamomi, the destructive and widespread oomycete causing ink disease. The European chestnut (Castanea sativa), carrying little to no disease resistance, is currently threatened by the presence of the oomycete pathogen in forests, orchards and nurseries. Determining the genetic basis of P. cinnamomi resistance, for further selection of molecular markers and candidate genes, is a prominent issue for implementation of marker assisted selection in the breeding programs for resistance. In this study, the first interspecific genetic linkage map of C. sativa x C. crenata …

The Soybean Rfg1 Gene Restricts Nodulation By Sinorhizobium Fredii Usda193, Yinglun Fan, Jinge Liu, Shanhua Lyu, Qi Wang, Shengming Yang, Hongyan Zhu

Plant and Soil Sciences Faculty Publications

Sinorhizobium fredii is a fast-growing rhizobial species that can establish a nitrogen-fixing symbiosis with a wide range of legume species including soybeans (Glycine max). In soybeans, this interaction shows a high level of specificity such that particular S. fredii strains nodulate only a limited set of plant genotypes. Here we report the identification of a dominant gene in soybeans that restricts nodulation with S. fredii USDA193. Genetic mapping in an F2 population revealed co-segregation of the underlying locus with the previously cloned Rfg1 gene. The Rfg1 allele encodes a member of the Toll-interleukin receptor/nucleotide-binding site/leucine-rich repeat class of …

Reciprocal Effect Of Parental Lines On The Physiological Potential And Seed Composition Of Corn Hybrid Seeds, Juliana F. Santos, Lynnette M. A. Dirk, A. Bruce Downie, Mauricio F. G. Sanches, Roberval D. Vieira

Horticulture Faculty Publications

Obtaining corn hybrid seeds (Zea mays L.) with high vigour depends on the parental lines and the direction of the cross, and this relates to seed desiccation tolerance and composition. This research studied reciprocal crosses between pairs of proprietary, elite parent lines (L1 and L5; L2 and L4) producing hybrid seeds with different qualities attempting to correlate vigour with seed composition, focusing on storage proteins, starch and soluble sugar amounts. Four corn hybrid seed lots produced from reciprocal crosses were compared (HS 15 with HS 51, and HS 24 with HS 42) by assessing germination, vigour, and seedling emergence …

Identification Of Hydrated And Dehydrated Lipids And Protein Secondary Structures In Seeds Of Cotton (Gossypium Hirsutum) Line, Diwas Kumar Silwal, Nsoki Phambu, Bharat Pokharel, Ahmad Naseer Aziz

Chemistry Faculty Research

Cottonseeds from two parents (TM-1 and 3-79) and their 17 progeny (chromosomal substitution) lines were analyzed for various secondary structures of proteins and moisture content of lipids, separately in hulls and kernels. Fourier transform infrared spectroscopy (FTIR) was used on mature seeds from Upland cotton (G. hirsutum) progeny lines and parents. Based on secondary structures of proteins and hydration levels of lipids, differences were observed among the cottonseeds. The two progeny lines – CS-B12sh and CS-B22sh retained lipid moisture content and protein secondary structures similar to both parents, while CS-B06, CS-B15sh and CS-B16 remained distinct from either parent. On the …

Effects Of Cultivar And Maternal Environment On Seed Quality In Vicia Sativa, Rong Li, Lijun Chen, Yanpei Wu, Rui Zhang, Carol C. Baskin, Jerry M. Baskin, Xiaowen Hu

Biology Faculty Publications

Production of high quality seeds is of fundamental importance for successful crop production. However, knowledge of the effects of increased temperature resulting from global warming on seed quality of alpine species is limited. We investigated the effect of maternal environment on seed quality of three cultivars of the leguminous forage species Vicia sativa, giving particular attention to temperature. Plants of each cultivar were grown at 1700 and 3000 m a.s.l., and mass, germination, electrical conductivity (EC) of leakage and longevity were determined for mature seeds. Seeds of all three cultivars produced at the low elevation had a significantly lower …