Plant Breeding and Genetics Commons^™

Production Of Tocotrienols In Seeds Of Cotton (Gossypium Hirsutum L.) Enhances Oxidative Stability And Offers Nutraceutical Potential, Shanmukh S. Salimath, Trevor B. Romsdahl, Anji Reddy Konda, Wei Zhang, Edgar B. Cahoon, Michael K. Dowd, Thomas C. Wedegaertner, Kater D. Hake, Kent D. Chapman

Center for Plant Science Innovation: Faculty Publications

Upland cotton (Gossypium hirsutum L.) is an economically important multi-purpose crop cultivated globally for fibre, seed oil and protein. Cottonseed oil also is naturally rich in vitamin E components (collectively known as tocochromanols), with a- and c-tocopherols comprising nearly all of the vitamin E components. By contrast, cottonseeds have little or no tocotrienols, tocochromanols with a wide range of health benefits. Here, we generated transgenic cotton lines expressing the barley (Hordeum vulgare) homogentisate geranylgeranyl transferase coding sequence under the control of the Brassica napus seed-specific promoter, napin. Transgenic cottonseeds had ~twofold to threefold increases in the accumulation of total vitamin …

Resistance To Aflatoxin Accumulation In Maize Mediated By Host-Induced Gene Silencing Of Aspergillus Flavus Alkaline Protease And O-Methyltransferase-A Genes, Olanike Omotola Omolehin

LSU Doctoral Dissertations

Aspergillus flavus is a soil-borne fungal pathogen that infects maize and produces aflatoxins. In the current study, portions of the alkaline protease (alk) and the O-methyl transferase (omtA) genes, which are key in A. flavus virulence and aflatoxin biosynthesis, respectively, were targeted for suppression through an RNAi (RNA interference) approach known as Host-Induced Gene Silencing (HIGS). Separate RNAi vectors were designed to carry regions of the alk and omtA gene fragments (Alk-RNAi and OmtA-RNAi) and introduced into B104 maize zygotic embryos. Eight and six transformation events were positive for the alk and omtA transgene, respectively. …

Molecular-Assisted Breeding For Soybean With High Oleic/Low Linolenic Acid And Elevated Vitamin E In The Seed Oil, Katherine Hagely, Anji Reddy Konda, Jeong-Hwa Kim, Edgar B. Cahoon, Kristin Bilyeu

Center for Plant Science Innovation: Faculty Publications

The uses of vegetable oils are determined by functional properties arising from their chemical composition. Soybean oil was previously used in margarines and baked foods after partial hydrogenation to achieve heat and oxidative stability. This process, however, generates trans fats that are now excluded from food use because of cardiovascular health risks. Also present in soybean oil are the anti-oxidant tocopherols, with α- tocopherol (vitamin E) typically present as a minor component compared to γ-tocopherol. Genetic improvement of the fatty acid profile and tocopherol profile is an attractive solution to increase the functional and health qualities of soybean oil. The …

Chile Pepper Variety Evaluation At Four Sites Across Minnesota And Wisconsin, 2020, Charlie Rohwer, Natalie Hoidal, Cala Farm, Agua Gorda Farm Cooperative, Jarl's Produce

Midwest Vegetable Trial Reports

Latinx fresh market vegetable farmers in Minnesota face numerous barriers to market access. Many Latinx farmers live in rural communities where farmers market sales are low, and opportunities for wholesale are limited. The growers who make up Shared Ground Farmers’ Cooperative have developed an innovative cooperative marketing system to aggregate produce and sell it in Twin Cities markets, but many of the individual farmers involved also wish to find ways to keep the produce they grow in their own communities. This project emerged through a series of discussions about finding niche markets for culturally relevant crops in small-town Mexican restaurants …

72-H Diurnal Rna-Seq Analysis Of Fully Expanded Third Leaves From Maize, Sorghum, And Foxtail Millet At 3-H Resolution, Xianjun Lai, Claire Bendix, Yan Zhang, James Schnable, Frank G. Harmon

Center for Plant Science Innovation: Faculty Publications

Objectives: The purpose of this data set is to capture the complete diurnal (i.e., daily) transcriptome of fully expanded third leaves from the C4 panacoid grasses sorghum (Sorghum bicolor), maize (Zea mays), and foxtail millet (Setaria italica) with RNA-seq transcriptome profiling. These data are the cornerstone of a larger project that examined the conservation and divergence of gene expression networks within these crop plants. This data set focuses on tem- poral changes in gene expression to identify the network architecture responsible for daily regulation of plant growth and metabolic activities. The power of this data set is fine temporal resolution …

Plasma Membrane Fluidity: An Environment Thermal Detector In Plants, Dora L. Cano-Ramirez, Laura Carmona-Salazar, Francisco Morales-Cedillo, Jorge Ramírez-Salcedo, Edgar B. Cahoon, Marina Gavilanes-Ruíz

Center for Plant Science Innovation: Faculty Publications

The lipid matrix in cell membranes is a dynamic, bidimensional array of amphipathic molecules exhibiting mesomorphism, which contributes to the membrane fluidity changes in response to temperature fluctuation. As sessile organisms, plants must rapidly and accurately respond to environmental thermal variations. However, mechanisms underlying temperature perception in plants are poorly understood. We studied the thermal plasticity of membrane fluidity using three fluorescent probes across a temperature range of -5 to 41 °C in isolated microsomal fraction (MF), vacuolar membrane (VM), and plasma membrane (PM) vesicles from Arabidopsis plants. Results showed that PM were highly fluid and exhibited more phase transitions …

Complete Mitogenomes Of Two Aragoa Species And Phylogeny Of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes And The Nuclear Ribosomal Rna Repeat, Jeffrey P. Mower, Lilly Hanley, Kirsten Wolff, Natalia Pabón-Mora, Favio González

Center for Plant Science Innovation: Faculty Publications

Aragoa, comprising 19 high-altitude North Andean species, is one of three genera in the Plantagineae (Plantaginaceae, Lamiales), along with Littorella and Plantago. Based primarily on plastid data and nuclear ITS, Aragoa is sister to a clade of Littorella + Plantago, but Plantagineae relationships have yet to be assessed using multigene datasets from the nuclear and mitochondrial genomes. Here, complete mitogenomes were assembled for two species of Aragoa (A. abietina and A. cleefii). The mitogenomes of both species have a typical suite of genes for 34 proteins, 17 tRNAs, and three rRNAs. The A. abietina mitogenome …

Inmut-Finder: A Software Tool For Insertion Identification In Mutagenesis Using Nanopore Long Reads, Rui Song, Ziyao Wang, Hui Wang, Han Zhang, Xuemeng Wang, Hanh Nguyen, David Holding, Bin Yu, Tom Clemente, Shangang Jia, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Biological mutagens (such as transposon) with sequences inserted, play a crucial role to link observed phenotype and genotype in reverse genetic studies. For this reason, accurate and efficient software tools for identifying insertion sites based on the analysis of sequencing reads are desired. Results: We developed a bioinformatics tool, a Finder, to identify genome-wide Insertions in Mutagenesis (named as “InMut-Finder”), based on target sequences and flanking sequences from long reads, such as Oxford Nanopore Sequencing. InMut-Finder succeeded in identify > 100 insertion sites in Medicago truncatula and soybean mutants based on sequencing reads of whole-genome DNA or enriched …

3d Reconstruction Identifies Loci Linked To Variation In Angle Of Individual Sorghum Leaves, Michael C. Tross, Mathieu Gaillard, Mackenzie Zwiener, Chenyong Miao, Ryleigh J. Grove, Bosheng Li, Bedrich Benes, James C. Schnable

Center for Plant Science Innovation: Faculty Publications

Selection for yield at high planting density has reshaped the leaf canopy of maize, improving photosynthetic productivity in high density settings. Further optimization of canopy architecture may be possible. However, measuring leaf angles, the widely studied component trait of leaf canopy architecture, by hand is a labor and time intensive process. Here, we use multiple, calibrated, 2D images to reconstruct the 3D geometry of individual sorghum plants using a voxel carving based algorithm. Automatic skeletonization and segmentation of these 3D geometries enable quan- tification of the angle of each leaf for each plant. The resulting measurements are both heritable and …

Somatic Variants For Seed And Fruit Set In Grapevine, Laura Costantini, Paula Moreno-Sanz, Chinedu Charles Nwafor, Silvia Lorenzi, Annarita Marrano, Fabiana Cristofolini, Elena Gottardini, Stefano Raimondi, Paola Ruffa, Ivana Gribaudo, Anna Schneider, Maria Stella Grando

Center for Plant Science Innovation: Faculty Publications

Background: Grapevine reproductive development has direct implications on yield. It also impacts on berry and wine quality by affecting traits like seedlessness, berry and bunch size, cluster compactness and berry skin to pulp ratio. Seasonal fluctuations in yield, fruit composition and wine attributes, which are largely driven by climatic factors, are major challenges for worldwide table grape and wine industry. Accordingly, a better understanding of reproductive processes such as gamete development, fertilization, seed and fruit set is of paramount relevance for managing yield and quality. With the aim of providing new insights into this field, we searched for clones with …

The Study Of Ppal And Its Role In The Development Of Physcomitrella Patens, Susana Perez-Martinez, Christine Chen, Mark P. Running

Undergraduate Arts and Research Showcase

Protein Prenylation is the addition of lipids to select proteins that play a key role during the development of plants. There are three enzymes that play a role in protein prenylation: protein farnesyltransferase (PFT), protein geranylgeranyl-transferase-I (PGGT), and Rab geranylgeranyltransferase (Rab-GGT). However, there is a protein called PPAL that has a similar alpha subunit of PFT, PGGT, and RAB-GGT but its biochemical function is unknown. Physconmitrella patens, a type of moss, was chosen to explore the role of PpPPAL in the development process. There are two copies of PPAL found in moss. PPAL1 and PPAL2. To study the role …

A Comparison Of Media To Determine Optimal Growth In Aquaponics: Prg Virtual Showcase January 2021, Rachel L. Fogle, Andrea Nagy, Claribel Asare, Steven Berry, Jordan Brown, Aquila Durham-Lewis

Harrisburg University Presidential Research Grants

No abstract provided.

Identification Of Loci Influencing Teosinte Crossing Barrier 1 (Tcb1) Efficacy In Maize By Quantitative Trait Loci (Qtl) Mapping And Genome-Wide Association Study (Gwas), Namrata Maharjan

Electronic Theses and Dissertations

Pollen cross-contamination has been a major problem for maize breeders. Various mechanical methods applied to avoid the contaminations are ineffective. The genetic factors related to maize fertilization can be used to develop an effective method to prevent pollen contamination. Pollen rejection ability controlled by Teosinte crossing barrier 1 (Tcb1) is such a genetic system. Silks possessing dominant Tcb1-s reject pollen possessing the recessive allele (tcb1). Successful fertilization occurs when Tcb1-s pollen falls upon tcb1 silks. The efficacy of dominant Tcb1-s was, however, reduced when repeatedly backcross with maize inbred lines, which suggests that there are modifiers to Tcb1-s. To find …

A Biocontrol Pesticide Derived From Mycovirus-Infected Sclerotinia Sclerotiorum Can Induce Plant Resistance, Connor Pedersen

Electronic Theses and Dissertations

Soybean leaf-associated gemycircularvirus-1 (SlaGemV-1) is a novel mycovirus discovered through the metagenomic sequencing of soybean leaves which is capable of inducing hypovirulence in the highly pathogenic fungus Sclerotinia sclerotiorum. RNASeq analysis techniques were used to determine the transcriptional changes caused by the infection of virus in S. sclerotiorum, as well as the transcriptional changes in Glycine max caused by the colonization of hypovirulent, SlaGemV-1-infected S. sclerotiorum. RNASeq results indicate that viral infection leading to hypovirulence may attenuate expression of genes relating to cell wall synthesis, microtubule formation, and metabolism of steroids and natural antibiotics. Cytochrome P450-related genes, kinesin domain genes, …

Genome-Wide Association Analysis Permits Characterization Of Stagonospora Nodorum Blotch (Snb) Resistance In Hard Winter Wheat, Rami Altameemi

Electronic Theses and Dissertations

Stagonospora nodorum blotch (SNB) is an economically important wheat disease caused by the necrotrophic fungus Parastagonospora nodorum. SNB resistance in wheat is controlled by several quantitative trait loci (QTLs). Thus, the identifying of novel resistance/susceptibility QTLs is crucial for continuous improvement of the SNB resistance. Here, the hard winter wheat association mapping panel (HWWAMP) comprising accessions from breeding programs in the Great Plains region of the US, was evaluated for SNB resistance and necrotrophic effectors (NEs) sensitivity at the seedling stage. A genome-wide association study (GWAS) was performed to identify singlenucleotide polymorphism (SNP) markers associated with SNB resistance and …

Genome Wide Association Study On Kernel Size In Avena Sativa (Oats), Koryne Ann Carlson

Electronic Theses and Dissertations

Kernel size is a vital milling characteristic for Avena sativa L. (oats), with larger kernel size being desirable for milling facilities. This project evaluated the effect of genotype, environment, and their genotype by environment interaction (G by E) on kernel width, length, and area for a set of 27 oat cultivars grown at multiple locations in South Dakota over three years. Findings indicated significant differences among oat varieties, and that environment had a substantial effect on kernel size. However, we did not see a strong influence of genotype by environment interaction on kernel size. The lack of impact for genotype …

Exploring Quantitative Trait Loci Mapping For Bud Fruitfulness And Bud Break Traits In Grapevine F2 Population, Seyma Bozkus

Electronic Theses and Dissertations

Grapevine (Vitis vinifera L. subsp. vinifera) is one of the most important and valuable fruit crops around the world and grape industries in the USA have been growing increasingly regarding the demand for highly productive and quality grapes that can be grown in the cold region around the USA. The development of new cultivars with these features is performed through grape breeding with the help of quantitative trait loci (QTL) mapping, marker-assisted selection (MAS), and and other technologies. In this study, we evaluated grapevine bud fruitfulness by position and bud break in controlled conditions. These traits are multi-genetic and understanding …

Genetic Structure Of Pseudoroegneria Spicata In The Northern Palouse Prairie And Channeled Scablands, Ethan Timothy Bean

EWU Masters Thesis Collection

Establishing genetically diverse communities that can adapt to dynamic selective pressures is crucial in ecological restoration. However, the genetic structure of native plant species used for restoration is often poorly understood. Pseudoroegneria spicata, is a keystone species of the Inland Northwestern US that has become a staple in commercially available restoration seed mixes. It is abundant in remnant prairies of the endangered Palouse Prairie ecoregion, which is characterized by rolling hills of deep loess soil. Less than 1% of native Palouse Prairie is left, due to agricultural conversion, with remnants highly fragmented and isolated. P. spicata is also common in …

Studies On Genetic Characterization Of Agronomic Traits And Disease Resistance In Bread Wheat, Jyotirmoy Halder

Electronic Theses and Dissertations

A steady increase in wheat yield is vital to feed the continuously rising world population. Systematic exploitation of wheat germplasm and a better understanding of the underlying genetic control could be pivotal in accelerating the genetic gain for yield and disease management. Various modern techniques such as genome-wide association study (GWAS), genomic selection (GS), fine mapping, and cloning can expedite wheat improvement and broaden our understanding of the complex wheat genome. In the first objective of this study, we evaluated the Watkins core set of 121 landrace cultivars (LCs) to identify novel sources of resistance against the tan spot, Stagonospora …

Arkansas Corn And Grain Sorghum Performance Tests 2020, J. F. Carlin, R. D. Bond, R. B. Morgan

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

The 2020 corn performance tests contained 85 hybrids and were conducted at the Northeast Research and Extension Center (NEREC) at Keiser, the Lon Mann Cotton Research Station (LMCRS) near Marianna, the Bell Farming Company (BFC) near Des Arc, the Pine Tree Research Station (PTRS) near Colt, the Rohwer Research Station (RRS) near Rohwer, and the Rice …