Other Plant Sciences Commons^™

Plectus Of The Prairie: A Case Study Of Taxonomic Resolution From A Nematode Biodiversity Survey, Abigail Borgmeier, Kaitlin Gattoni, Timothy Harris, Rebecca Higgins, Peter Mullin, Dorota Porazinska, Kirsten S. Powers, David A. Wedin, Tom Powers

Department of Plant Pathology: Faculty Publications

Taxonomic resolution is a critical component of biodiversity assessments. In this case study, we examined a single taxon within a larger study of nematode diversity to evaluate the taxonomic resolution of different diversity assessment methods. The selected taxon was the microbial-feeding genus Plectus, a group considered to include multiple cosmopolitan species. The methods included a morphological evaluation by light microscopy, Sanger sequencing of PCR amplicons of COI and 18S gene regions, and 18S metabarcoding sequencing. The study sites were 15 remnant tallgrass prairie plots in eastern Nebraska. In the morphological analysis, we observed two basic morphotypes, a short-tailed form …

Cytotoxic Activity Of Non-Specific Lipid Transfer Protein (Nsltp1) From Ajwain (Trachyspermum Ammi) Seeds, Saud O. Alshammari, Taibah Aldakhil, Qamar A. Alshammari, David Salehi, Aftab Ahmed

Pharmacy Faculty Articles and Research

Background

Trachyspermum ammi, commonly known as Ajwain, is a member of the Apiaceae family. It is a therapeutic herbal spice with diverse pharmacological properties, used in traditional medicine for various ailments. However, all previous studies were conducted using small molecule extracts, leaving the protein’s bioactivity undiscovered.

Aim

The current study aimed to demonstrate the cytotoxic activity of Ajwain non-specific lipid transfer protein (nsLTP1) in normal breast (MCF10A), breast cancer (MCF-7), and pancreatic cancer (AsPC-1) cell lines. Also, to evaluate its structural stability in human serum as well as at high temperature conditions.

Methods

The cytotoxic activity of Ajwain nsLTP1 was …

Reassessment Of Species Boundaries And Phylogenetic Relationships In The Desmodium Ciliare Complex (Fabaceae) Using Morphological And Dna Data, Joshua Wilkinson

Master's Theses

Desmodium (Beggar's ticks) is a generally weedy genus of approximately 280 species in the angiosperm family Fabaceae (Subfamily Papilionoideae, Tribe Desmodieae) characterized by indehiscent loments constricted into segments. Within the Southeastern U.S., the Desmodium ciliare group is one of two Desmodium species complexes that have been historically difficult, with much argument about the limits of species and the role of hybridization. There are three commonly recognized species within the D. ciliare group, D. ciliare (Muhl. ex Willd.) DC., Desmodium marilandicum (L.) DC., and D. obtusum (Muhl. ex Willd.) DC., with two supposed hybrids in addition to the …

Nebline, May 2022

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Insect and Disease Control for Organic Vegetable Gardeners by Sarah Browning

Garden Guide: Things to Do This Month

An Egg-cellent Choice!

Recipe of the Month: Sweet Potatoes and Black Bean Hash by Alyssa Havlovic

It’s Time for Private Well Maintenance by Becky Scheurman

Cash Rent Survey

Composting Demonstrations

Monarchs, Migration & Milkweed by Kait Chapman

Getting Outside to Play by Hayley Jackson

4th Graders Learn About “Farm to Fork” at Ag Festival

4-H Clubs Helped at Kiwanis Karnival

Support Local 4-H Youth During “Give to Lincoln Day”

4-H Achievement Celebration

Heart of 4-H Volunteer Award: Rick Waldron

3 Lancaster County …

An In Vitro Approach To Identify Sources Of Resistance To Fusarium Wilt Caused By Fusarium Oxysporum F. Sp. Vasinfectum Race 4 In Domesticated Cotton, Stephen Parris

All Theses

Cotton lint produced by the plants Gossypium barbadense L. (pima cotton) and Gossypium hirsutum L. (upland cotton) is the world’s leading source of renewable textiles. The fibers of the cotton plant have been woven so intricately into our society that it would be hard to imagine life without their products. Cotton breeders must then not only continue to work to improve the quality of cotton fibers (fineness, strength, dyability, etc.) but also to improve the resilience of the plant against biotic and abiotic factors. Fusarium oxysporum f. sp. vasinfectum race 4 (FOV4) is an aggressive fungal pathogen, which causes Fusarium …

Integration Of Medicinal Fungi Into The Heber W. Youngken Jr. Medicinal Garden, Anthony Makashov

Senior Honors Projects

The Heber W. Youngken Jr. Medicinal Garden is a one-of-a-kind resource that houses close to 300 medicinal plants and is filled to the brim with educational opportunities. For the College of Pharmacy, this garden has been the centerpiece of many classes taught here at the University of Rhode Island and has roots as a physical teaching space. While the garden contains a plethora of plant species, medicinal fungi have yet to have been included in the garden, despite their relevance as both sources of pharmaceutical drug discovery and as natural products with inherent physiological benefits themselves. The purpose of my …

Chromosome-Level Thlaspi Arvense Genome Provides New Tools For Translational Research And For A Newly Domesticated Cash Cover Crop Of The Cooler Climates, Adam Nunn, Isaac Rodríguez-Arévalo, Zenith Tandukar, Katherine Anna Frels, Adrián Contreras-Garrido, Pablo Carbonell-Bejerano, Panpan Zhang, Daniela Ramos Cruz, Katharina Jandrasits, Christa Lanz, Anthony Brusa, Marie Mirouze, Kevin Dorn, David W. Galbraith, Brice A. Jarvis, John C. Sedbrook, Donald L. Wyse, Christian Otto, David Langenberger, Peter F. Stadler, Detlef Weigel, M. David Marks, James A. Anderson, Claude Becker, Ratan Chopra

Department of Agronomy and Horticulture: Faculty Publications

Thlaspi arvense (field pennycress) is being domesticated as a winter annual oilseed crop capable of improving ecosystems and intensifying agricultural productivity without increasing land use. It is a selfing diploid with a short life cycle and is amenable to genetic manipulations, making it an accessible field-based model species for genetics and epigenetics. The availability of a high-quality reference genome is vital for understanding pennycress physiology and for clarifying its evolutionary history within the Brassicaceae. Here, we present a chromosome-level genome assembly of var. MN106-Ref with improved gene annotation and use it to investigate gene structure differences between two accessions (MN108 …

Agricultural Intensification Can Help Protect The Amazon Forest And Reduce Global Warming / Protecting The Amazon Forest And Reducing Global Warming Via Agricultural Intensification, Fabio R. Marin, Alencar J. Zanon, Juan P. Monzon, José F. Andrade, Evandro H.F.M. Silva, Gean L. Richter, Luis A.S. Antolin, Bruna S.M.R. Ribeiro, Giovanna G. Ribas, Rafael Battisti, Alexandre B. Heinemann, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

The Amazon basin includes 550 M ha covered with rainforests, with 60% of this area being in Brazil. Conversion of rainforest for soybean production raises concerns about the degree to which Brazil can reconcile production and environmental goals. Here we investigated the degree to which intensification could help Brazil produce more soybean without further encroachment of the Amazon Forest. Our analysis shows that continuation of current trends in soybean yield and area would lead to conversion of additional 5.7 M ha of forests and savannas during the next 15 years, with an associated 2550 Mt of CO2eq released into the …

Climate Change And Management Impacts On Soybean N Fixation, Soil N Mineralization, N2O Emissions, And Seed Yield, Elvis F. Elli, Ignacio A. Ciampitti, Michael J. Castellano, Larry C. Purcell, Seth Naeve, Patricio Grassini, Nicolas C. La Menza, Luiz Moro Rosso, André F. De Borja Reis, Péter Kovács, Sotirios V. Archontoulis

Department of Agronomy and Horticulture: Faculty Publications

Limited knowledge about how nitrogen (N) dynamics are affected by climate change, weather variability, and crop management is a major barrier to improving the productivity and environmental performance of soybean-based cropping systems. To fill this knowledge gap, we created a systems understanding of agroecosystem N dynamics and quantified the impact of controllable (management) and uncontrollable (weather, climate) factors on N fluxes and soybean yields. We performed a simulation experiment across 10 soybean production environments in the United States using the Agricultural Production Systems sIMulator (APSIM) model and future climate projections from five global circulation models. Climate change (2020–2080) increased N …

Two Fingerprinting Sets For Humulus Lupulus Based On Kasp And Microsatellite Markers, Mandie Driskill, Katie Pardee, Kim E. Hummer, Jason D. Zurni, Keenan L. Amundsen, Annette Wiles, Claudia Wiedowi, Josef Patza, John A. Henningi, Nahla V. Bassili

Department of Agronomy and Horticulture: Faculty Publications

Verification of clonal identity of hop (Humulus lupulus L.) cultivars within breeding programs and germplasm collections is vital to conserving genetic resources. Accurate and economic DNA-based tools are needed in dioecious hop to confirm identity and parentage, neither of which can be reliably determined from morphological observations. In this study, we developed two fingerprinting sets for hop: a 9-SSR fingerprinting set containing high-core repeats that can be run in a single PCR reaction and a kompetitive allele specific PCR (KASP) assay of 25 single nucleotide polymorphisms (SNPs). The SSR set contains a sex-linked primer pair, HI-AGA7, that was used …

Two Magnaporthe Appressoria-Specific (Mas) Proteins, Momas3 And Momas5, Are Required For Suppressing Host Innate Immunity And Promoting Biotrophic Growth In Rice Cells, Ziwen Gong, Na Ning, Zhiqiang Li, Xin Xie, Richard Wilson, Wende Liu

Department of Plant Pathology: Faculty Publications

In the devastating rice blast fungus Magnaporthe oryzae, six Magnaporthe appressoria-specific (MAS) proteins are encoded by MoGAS1, MoGAS2 and MoMAS3–MoMAS6. MoGAS1 and MoGAS2 were previously characterized as M. oryzae virulence factors; however, the roles of the other four genes are unknown. Here, we found that, although the loss of any MAS gene did not affect appressorial formation or vegetative growth, ΔMomas3 and ΔMomas5 mutant strains (but not the others) were reduced in virulence on susceptible CO-39 rice seedlings. Focusing on ΔMomas3 and ΔMomas5 mutant strains, we found that they could penetrate …

Variation In Bacterial Community Structure Under Long-Term Fertilization, Tillage, And Cover Cropping In Continuous Cotton Production, Ning Duan, Lidong Li, Xiaolong Liang, Aubrey Fine, Jie Zhuang, Mark Radosevich, Sean M. Schaeffer

Department of Agronomy and Horticulture: Faculty Publications

Agricultural practices alter the structure and functions of soil microbial community. However, few studies have documented the alterations of bacterial communities in soils under long-term conservation management practices for continuous crop production. In this study, we evaluated soil bacterial diversity using 16S rRNA gene sequencing and soil physical and chemical properties within 12 combinations of inorganic N fertilization, cover cropping, and tillage throughout a cotton production cycle. Soil was collected from field plots of the West Tennessee Agriculture Research and Education Center in Jackson, TN, United States. The site has been under continuous cotton production for 38 years. A total …

Fine Mapping And Cloning Of The Major Seed Protein Quantitative Trait Loci On Soybean Chromosome 20, Christina E. Fliege, Russell A. Ward, Pamela Vogel, Hanh Nguyen, Truyen Quach, Ming Guo, João Paulo Gomes Viana, Lucas Borges Dos Santos, James Specht, Thomas Clemente, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is a unique crop species because it has high levels of both protein and oil in its seed. Of the many quantitative trait loci (QTL) controlling soybean seed protein content, alleles of the cqSeed protein-003 QTL on chromosome 20 exert the greatest additive effect. The high-protein allele exists in both cultivated and wild soybean (Glycine soja Siebold & Zucc.) germplasm. Our objective was to fine map this QTL to enable positional-based cloning of its underlying causative gene(s). Fine mapping was achieved by developing and testing a series of populations in which the chromosomal region surrounding …

Microbiome Variation Across Populations Of Desert Halophyte Zygophyllum Qatarensis, Abdul Latif Khan, Lucas Dantas Lopes, Saqib Bilal, Sajjad Asaf, Kerri M. Crawford, Venkatesh Balan, Ahmed Al-Rawahi, Ahmed Al-Harrasi, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Microbial symbionts play a significant role in plant health and stress tolerance. However, few studies exist that address rare species of core-microbiome function during abiotic stress. In the current study, we compared the microbiome composition of succulent dwarf shrub halophyte Zygophyllum qatarensis Hadidi across desert populations. The results showed that rhizospheric and endosphere microbiome greatly varied due to soil texture (sandy and gravel). No specific bacterial amplicon sequence variants were observed in the core-microbiome of bulk soil and rhizosphere, however, bacterial genus Alcaligenes and fungal genus Acidea were abundantly distributed across root and shoot endospheres. We also analyzed major nutrients …

Microbiome Variation Across Populations Of Desert Halophyte Zygophyllum Qatarensis, Abdul Latif Khan, Lucas Dantas Lopes, Saqib Bilal, Sajjad Asaf, Kerri M. Crawford, Venkatesh Balan, Ahmed Al-Rawahi, Ahmed Al-Harrasi, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Microbial symbionts play a significant role in plant health and stress tolerance. However, few studies exist that address rare species of core-microbiome function during abiotic stress. In the current study, we compared the microbiome composition of succulent dwarf shrub halophyte Zygophyllum qatarensis Hadidi across desert populations. The results showed that rhizospheric and endosphere microbiome greatly varied due to soil texture (sandy and gravel). No specific bacterial amplicon sequence variants were observed in the core-microbiome of bulk soil and rhizosphere, however, bacterial genus Alcaligenes and fungal genus Acidea were abundantly distributed across root and shoot endospheres. We also analyzed major nutrients …

A Global Soil Spectral Calibration Library And Estimation Service, Keith D. Sheperd, Rich Ferguson, David Hoover, Fenny Van Egmond, Jonathan Sanderman, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

There is growing global interest in the potential for soil reflectance spectroscopy to fill an urgent need for more data on soil properties for improved decision-making on soil security at local to global scales. This is driven by the capability of soil spectroscopy to estimate a wide range of soil properties from a rapid, inexpensive, and highly reproducible measurement using only light. However, several obstacles are preventing wider adoption of soil spectroscopy. The biggest obstacles are the large variation in the soil analytical methods and operating procedures used in different laboratories, poor reproducibility of analyses within and amongst laboratories and …

Southeast Asia Must Narrow Down The Yield Gap To Continue To Be A Major Rice Bowl, Shen Yuan, Alexander M. Stuart, Alice G. Laborte, Juan I. Rattalino Edreira, Achim Dobermann, Le Vu Ngoc Kien, Lưu Thị Thúy, Kritkamol Paothong, Prachya Traesang, Khin Myo Tint, Su Su San, Marcelino Q. Villafuerte Ii, Emma D. Quicho, Anny Ruth P. Pame, Rathmuny Then, Rica Joy Flor, Neak Thon, Fahmuddin Agus, Nurwulan Agustiani, Nanyan Deng, Tao Li, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Southeast Asia is a major rice-producing region with a high level of internal consumption and accounting for 40% of global rice exports. Limited land resources, climate change and yield stagnation during recent years have once again raised concerns about the capacity of the region to remain as a large net exporter. Here we use a modelling approach to map rice yield gaps and assess production potential and net exports by 2040. We find that the average yield gap represents 48% of the yield potential estimate for the region, but there are substantial differences among countries. Exploitable yield gaps are relatively …

Editorial: Genomics-Enabled Triticeae Improvement, Xue-Feng Ma, Xianchun Xia, Shuyu Liu, P. Stephen Baenziger, Hakan Özkan

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.

Quantifying And Manipulating The Angles Of Light In Experimental Measurements Of Plant Gas Exchange, Z. Carter Berry, Jerry Larue, Gregory R. Goldsmith

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Diffuse light has been shown to alter plant leaf photosynthesis, transpiration and water-use efficiency. Despite this, the angular distribution of light for the artificial light sources used with common gas exchange systems is unknown. Here, we quantify the angular distribution of light from common gas exchange systems and demonstrate the use of an integrating sphere for manipulating those light distributions. Among three different systems, light from a 90° angle perpendicular to the leaf surface (±5.75°) was <25% of the total light reaching the leaf surface. The integrating sphere resulted in a greater range of possible distributions from predominantly direct light (i.e., >40% of light from a 90 ± 5.75° angle perpendicular to the leaf surface) to almost entirely diffuse (i.e., light from an even distribution …

No-Till Farming And Greenhouse Gas Fluxes: Insights From Literature And Experimental Data, S. J. Ruis, H. Blanco-Canqui, P. J. Jasa, V. L. Jin

Department of Agronomy and Horticulture: Faculty Publications

Tillage intensity may differently impact gaseous losses of C and N to the atmosphere, but data from long-term experiments are relatively few. Yet, this information is needed to better understand C and N losses and gains in agricultural systems. The objective of this study was to determine how tillage intensity affects soil greenhouse gas (GHG) fluxes (CO₂, N₂O, and CH₄) by comparing experimental data from moldboard plow (MP), chisel plow (CP), double disk (DD), and no-till (NT) soils after 38–40 yr of management in a rainfed corn (Zea mays L.)- soybean (Glycine …