Efficacy Of Singular And Stacked Brown Midrib 6 And 12 In The Modification Of Lignocellulose And Grain Chemistry,
2010
USDA-ARS
Efficacy Of Singular And Stacked Brown Midrib 6 And 12 In The Modification Of Lignocellulose And Grain Chemistry, Scott E. Sattler, Deanna L. Funnell-Harris, Jeffrey F. Pedersen
Department of Agronomy and Horticulture: Faculty Publications
In sorghum, brown midrib (bmr) 6 and 12 impair the last two steps of monolignol synthesis. bmr genes were introduced into grain sorghum to improve the digestibility of lignocellulosic tissues for grazing or bioenergy uses following grain harvest. Near-isogenic grain sorghum hybrids (AWheatland X RTx430) were developed containing bmr6, bmr12, and the bmr6 bmr12 double mutant (stacked), and their impacts were assessed in a two-year field study. The bmr genes did not significantly impact grain or lignocellulosic tissue yield. Lignocellulosic tissue from bmr6, bmr12, and stacked hybrids had reduced lignin content and increased in …
Heterosis In Sweet Sorghum
And Selection Of A New Sweet Sorghum Hybrid
For Use In Syrup Production In Appalachia,
2010
University of Kentucky
Heterosis In Sweet Sorghum And Selection Of A New Sweet Sorghum Hybrid For Use In Syrup Production In Appalachia, T. W. Pfeiffer, M. J. Bitzer, J. J. Toy, Jeffrey F. Pedersen
Department of Agronomy and Horticulture: Faculty Publications
Although heterosis is well established in grain and forage sorghum [Sorghum bicolor (L.) Moench], reports of heterosis in sweet sorghum are limited to results from grain sorghum × sweet sorghum hybrids. Recent development of cytoplasmic male-sterile sweet sorghum lines allows creation of sweet sorghum hybrids for research and industry. Male sterility may also affect allocation of photosynthate to plant parts, creating the potential to increase sugar content in stems by eliminating seed as a sink. The objectives of this study were to compare performance of A3 cytoplasmic male-sterile lines and A3 cytoplasmic male-sterile hybrids to fertile B …
Influences Of Wind And Sandblasting On The
Endangered Blowout Penstemon,
2010
University of Nebraska-Lincoln
Influences Of Wind And Sandblasting On The Endangered Blowout Penstemon, James Stubbendieck, Cheryl D. Dunn, Heidi L. Hillhouse, L. M. Landholt
Department of Agronomy and Horticulture: Faculty Publications
Blowout penstemon Penstemon haydenii is a federally endangered species growing only in areas with active wind erosion in sand dunes of the central United States. This early seral species declines as the blowout habitat stabilizes, allowing later seral species to increase. Blowout penstemon populations and plant size declined in the 1990s when precipitation was higher than normal, resulting in reduced sand movement. We conducted a greenhouse experiment to determine whether blowing sand influenced vigor and persistence of blowout penstemon seedlings. Treatments were wind, sandblasting, wind with sandblasting, and a control. The wind treatment was a constant 14 km h–1 …
Fine Mapping Of The Scn Resistance Locus Rhg1-B
From Pi 88788,
2010
University of Illinois
Fine Mapping Of The Scn Resistance Locus Rhg1-B From Pi 88788, Myungsik Kim, David L. Hyten, Andrew F. Bent, Brian W. Diers
Department of Agronomy and Horticulture: Faculty Publications
Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is the most economically damaging soybean [Glycine max (L.) Merr.] pest in the USA and genetic resistance is a key component for its control. Although SCN resistance is quantitative, the rhg1 locus on chromosome 18 (formerly known as Linkage Group G) confers a high level of resistance. The objective of this study was to fi ne-map the rhg1-b allele that is derived from plant introduction (PI) 88788. F2 and F3 plants and F3:4 lines from crosses between SCN resistant and susceptible genotypes were tested with genetic markers to …
High-Throughput Snp Discovery And Assay
Development In Common Bean,
2010
USDA, Agricultural Research Service
High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan
Department of Agronomy and Horticulture: Faculty Publications
Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly. To this end we developed a method which couples sequences obtained from the Roche 454-FLX system (454) with the Illumina Genome Analyzer (GA) for high-throughput SNP discovery. Results: Using a multi-tier reduced representation library we discovered a total of …
Inoculation With Arbuscular Mycorrhizal Fungi Or
Crop Rotation With Mycorrhizal Plants Improves
The Growth Of Maize In Limed Acid Sulfate Soil,
2010
Nihon University
Inoculation With Arbuscular Mycorrhizal Fungi Or Crop Rotation With Mycorrhizal Plants Improves The Growth Of Maize In Limed Acid Sulfate Soil, Hasao Higo, Katsunori Isobe, Dong-Jin Kang, Kazuhiro Ujie, Rhae A. Drijber, Ryuichi Ishii
Department of Agronomy and Horticulture: Faculty Publications
Arbuscular mycorrhizal fungi (AMF) improve the uptake of immobile mineral nutrients such as phosphate, thereby improving plant growth. In acid sulfate soil (ASS), AMF spore density is generally low which impacts root colonization and phosphate uptake. Thus, inoculation may help increase AMF colonization of crops grown in ASS. AMF spore density decreases after cultivation of a non-host crop or bare fallow. In addition, preceding crops affect the growth and yield of subsequent crops. The production of AMF inocula requires AMF-compatible plants. The objective of the present study is to elucidate the effect of preceding crops on the persistence of inoculated …
Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538,
2010
University of Illinois
Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers
Department of Agronomy and Horticulture: Faculty Publications
The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from ‘Dowling’. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping …
Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean,
2010
USDA-ARS, Soybean Genomics and Improvement Lab., Beltsville, MD
Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan
Department of Agronomy and Horticulture: Faculty Publications
Simple sequence repeat (SSR) genetic markers, also referred to as microsatellites, function in map-based cloning and for marker-assisted selection in plant breeding. The objectives of this study were to determine the abundance of SSRs in the soybean genome and to develop and test soybean SSR markers to create a database of locus-specific markers with a high likelihood of polymorphism. A total of 210,990 SSRs with di-, tri-, and tetranucleotide repeats of five or more were identified in the soybean whole genome sequence (WGS) which included 61,458 SSRs consisting of repeat units of di- (≥10), tri- (≥8), and tetranucleotide (≥7). Among …
