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Comparative Genomics Of Five Valsa Species Gives Insights On Their Pathogenicity Evolution, Guangchao Sun, Shichang Xie, Lin Tang, Chao Zhao, Mian Zhang, Lili Huang 2022 Northwest A&F University, University of Nebraska-Lincoln,

Comparative Genomics Of Five Valsa Species Gives Insights On Their Pathogenicity Evolution, Guangchao Sun, Shichang Xie, Lin Tang, Chao Zhao, Mian Zhang, Lili Huang

Department of Agronomy and Horticulture: Faculty Publications

Valsa is a genus of ascomycetes within the Valsaceae family. This family includes many wood destructive pathogens such as the well known Valsa mali and Valsa pyri which cause canker diseases in fruit trees and threaten the global fruit production. Lack of genomic information of this family is impeding our understandings about their evolution and genetic basis of their pathogenicity divergence. Here, we report genome assemblies of Valsa malicola, Valsa persoonii, and Valsa sordida which represent close relatives of Valsa mali and Valsa pyri with different host preferences. Comparative genomics analysis revealed that segmental rearrangements, inversions, and translocations …


Comparative Genome Analysis Of Plant Ascomycete Fungal Pathogens With Different Lifestyles Reveals Distinctive Virulence Strategies, Yansu Wang, Jie Wu, Jiacheng Yan, Ming Guo, Lei Xu, Liping Hou, Quan Zou 2022 Shenzhen Polytechnic

Comparative Genome Analysis Of Plant Ascomycete Fungal Pathogens With Different Lifestyles Reveals Distinctive Virulence Strategies, Yansu Wang, Jie Wu, Jiacheng Yan, Ming Guo, Lei Xu, Liping Hou, Quan Zou

Department of Agronomy and Horticulture: Faculty Publications

Background: Pathogens have evolved diverse lifestyles and adopted pivotal new roles in both natural ecosystems and human environments. However, the molecular mechanisms underlying their adaptation to new lifestyles are obscure. Comparative genomics was adopted to determine distinct strategies of plant ascomycete fungal pathogens with different lifestyles and to elucidate their distinctive virulence strategies. Results: We found that plant ascomycete biotrophs exhibited lower gene gain and loss events and loss of CAZyme-encoding genes involved in plant cell wall degradation and biosynthesis gene clusters for the production of secondary metabolites in the genome. Comparison with the candidate effectome detected distinctive variations between …


Biobased Sprayable Mulch Films Suppressed Annual Weeds In Vegetable Crops, Eliott Gloeb, Sibel Irmak, Loren Isom, John L. Lindquist, Samuel E. Wortman 2022 University of Nebraska-Lincoln

Biobased Sprayable Mulch Films Suppressed Annual Weeds In Vegetable Crops, Eliott Gloeb, Sibel Irmak, Loren Isom, John L. Lindquist, Samuel E. Wortman

Department of Agronomy and Horticulture: Faculty Publications

Biobased sprayable mulch (BSM) films are a potential alternative to herbicides, polyethylene plastic mulch film, and hand weeding for specialty crops. We developed a series of BSM films using locally available biomaterials [including corn (Zea mays) starch, glycerol, keratin hydrolysate, corn gluten meal, corn zein, eggshells, and isolated soy (Glycine max) protein] and tested their effects on weeds and crop yield during a total of seven greenhouse or field trials between 2017 and 2019 in Nebraska, USA. Application rates of BSM films applied in pots (greenhouse), planting holes in plastic film (field), or bed tops (field) …


Mora De Castilla: Cadena De Valor Para Contribuir A La Competitividad De La Provincia Del Sumapaz En Cundinamarca (Colombia, Nelson Enrique Fonseca Carreño 2022 Universidad de Cundinamarca

Mora De Castilla: Cadena De Valor Para Contribuir A La Competitividad De La Provincia Del Sumapaz En Cundinamarca (Colombia, Nelson Enrique Fonseca Carreño

Ciencias Administrativas, Económicas y Contables

La cadena de valor es un instrumento utilizado en las empresas que consiste en analizar las actividades presentes en el ciclo productivo de un producto, con el propósito de identificar fuentes de ventaja competitiva. Bajo esta premisa, esta investigación pretende diagnosticar, caracterizar y diseñar la cadena de valor de la mora de Castilla (Rubus claucus) en la provincia de Sumapaz, Cundinamarca, a través de metodologías de diagnóstico. Se tuvo en cuenta el Modelo de desarrollo agroempresarial de la Cámara de Comercio de Bogotá (CCB), el cual identifica, caracteriza y define las necesidades de todos los agronegocios involucrados en la cadena …


The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey 2022 Grand Valley State University

The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey

Masters Theses

The ability of plants to absorb light through photoreceptors is essential for successful plant development. Phytochromes are important plant photoreceptors that detect and absorb red and far-red light and must function properly for plant survival. The Light Response BTB proteins (LRB) play an important role in a plant’s response to red light by degrading phytochrome B (PhyB) via the ubiquitin proteasome system (UPS). Although there has been a successful investigation into understanding the function of the LRBs, the function of a few regions within LRB proteins remains unknown, including a portion located on the N-terminal end of the protein, termed …


A Non-Destructive Approach For Measuring Rice Panicle-Level Photosynthetic Responses Using 3d-Image Reconstruction, Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Harel Bacher4, Zvi Peleg, Paul E. Staswick, Jason Hupp, Hongfeng Yu, Harkamal Walia 2022 University of Nebraska-Lincoln

A Non-Destructive Approach For Measuring Rice Panicle-Level Photosynthetic Responses Using 3d-Image Reconstruction, Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Harel Bacher4, Zvi Peleg, Paul E. Staswick, Jason Hupp, Hongfeng Yu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Background: Our understanding of the physiological responses of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impact on grain yield. This is primarily due to the lack of a suitable gas exchange methodology for panicles and non-destructive methods to accurately determine panicle surface area.

Results: To address these challenges, we have developed a custom panicle gas exchange cylinder compatible with the LiCor 6800 Infra-red Gas Analyzer. Accurate surface area measurements were determined using 3D panicle imaging to normalize the panicle-level photosynthetic measurements. We observed differential responses in …


Full Issue, 2022 Cal Poly Humboldt

Full Issue

Humboldt Journal of Social Relations

Full issue of volume 35 of HJSR


Bison Movements Change With Weather: Implications For Their Continued Conservation In The Anthropocene, Nicholas A. McMillan, Samuel D. Fuhlendorf, Barney Luttbeg, Laura E. Goodman, Craig A. Davis, Brady W. Allred, Robert G. Hamilton 2022 University of Nebraska-Lincoln

Bison Movements Change With Weather: Implications For Their Continued Conservation In The Anthropocene, Nicholas A. Mcmillan, Samuel D. Fuhlendorf, Barney Luttbeg, Laura E. Goodman, Craig A. Davis, Brady W. Allred, Robert G. Hamilton

Department of Agronomy and Horticulture: Faculty Publications

Animal movement patterns are affected by complex interactions between biotic and abiotic landscape conditions, and these patterns are being altered by weather variability associated with a changing climate. Some animals, like the American plains bison (Bison bison L.; hereafter, plains bison), are considered keystone species, thus their response to weather variability may alter ecosystem structure and biodiversity patterns. Many movement studies of plains bison and other ungulates have focused on point-pattern analyses (e.g., resource-selection) that have provided information about where these animals move, but information about when or why these animals move is limited. For example, information surrounding the …


Technical Note: On Uncertainties In Plant Water Isotopic Composition Following Extraction By Cryogenic Vacuum Distillation, Haoyu Diao, Philipp Schuler, Gregory R. Goldsmith, Rolf T. W. Siegwolf, Matthias Saurer, Marco M. Lehmann 2022 Swiss Federal Institute for Forest, Snow and Landscape Research WSL

Technical Note: On Uncertainties In Plant Water Isotopic Composition Following Extraction By Cryogenic Vacuum Distillation, Haoyu Diao, Philipp Schuler, Gregory R. Goldsmith, Rolf T. W. Siegwolf, Matthias Saurer, Marco M. Lehmann

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Recent studies have challenged the interpretation of plant water isotopes obtained through cryogenic vacuum distillation (CVD) based on observations of a large 2H fractionation. These studies have hypothesized the existence of an H-atom exchange between water and organic tissue during CVD extraction with the magnitude of H exchange related to relative water content of the sample; however, clear evidence is lacking. Here, we systematically tested the uncertainties in the isotopic composition of CVD-extracted water by conducting a series of incubation and rehydration experiments using isotopically depleted water, water at natural isotope abundance, woody materials with exchangeable H, and organic materials …


The Genome Of Orychophragmus Violaceus Provides Genomic Insights Into The Evolution Of Brassicaceaepolyploidizationandits Distinct Traits, Kang Zhang, Yinqing Yang, Xin Zhang, Lingkui Zhang, Yu Fu, Zhongwei Guo, Shumin Chen, Jian Wu, James C. Schnable, Keke Yi, Xiaowu Wang, Feng Cheng 2022 Chinese Academy of Agricultural Sciences

The Genome Of Orychophragmus Violaceus Provides Genomic Insights Into The Evolution Of Brassicaceaepolyploidizationandits Distinct Traits, Kang Zhang, Yinqing Yang, Xin Zhang, Lingkui Zhang, Yu Fu, Zhongwei Guo, Shumin Chen, Jian Wu, James C. Schnable, Keke Yi, Xiaowu Wang, Feng Cheng

Department of Agronomy and Horticulture: Faculty Publications

Orychophragmus violaceus, referred to as ‘‘eryuelan’’ (February orchid) in China, is an early-flowering ornamental plant. The high oil content and abundance of unsaturated fatty acids in O. violaceus seeds make it a potential high-quality oilseed crop. Here, we generated a whole-genome assembly for O. violaceus using Nanopore and Hi-C sequencing technologies. The assembled genome of O. violaceus was ~1.3 Gb in size, with 12 pairs of chromosomes. Through investigation of ancestral genome evolution, we determined that the genome of O. violaceus experienced a tetraploidization event from a diploid progenitor with the translocated proto-Calepineae karyotype. Comparisons between the reconstructed subgenomes …


Novel Single-Nucleotide Variants For Morpho-Physiological Traits Involved In Enhancing Drought Stress Tolerance In Barley, Ibrahim S. Elbasyoni, Shamseldeen Eltaher, Sabah Morsy, Alsayed M. Mashaheet, Ahmed M. Abdallah, Heba G. Ali, Samah A. Mariey, P. Stephen Baenziger, Katherine Anna Frels 2022 Damanhour University, University of Nebraska-Lincoln

Novel Single-Nucleotide Variants For Morpho-Physiological Traits Involved In Enhancing Drought Stress Tolerance In Barley, Ibrahim S. Elbasyoni, Shamseldeen Eltaher, Sabah Morsy, Alsayed M. Mashaheet, Ahmed M. Abdallah, Heba G. Ali, Samah A. Mariey, P. Stephen Baenziger, Katherine Anna Frels

Department of Agronomy and Horticulture: Faculty Publications

Barley (Hordeum vulgare L.) thrives in the arid and semi-arid regions of the world; never-theless, it suffers large grain yield losses due to drought stress. A panel of 426 lines of barley was evaluated in Egypt under deficit (DI) and full irrigation (FI) during the 2019 and 2020 growing seasons. Observations were recorded on the number of days to flowering (NDF), total chlorophyll content (CH), canopy temperature (CAN), grain filling duration (GFD), plant height (PH), and grain yield (Yield) under DI and FI. The lines were genotyped using the 9K Infinium iSelect single nucleotide polymorphisms (SNP) genotyping platform, which …


Genome‑Wide Association Analyses Of Leaf Rust Resistance In Cultivated Emmer Wheat, Dhondup Lhamo, Qun Sun, Qijun Zhang, Xuehui Li, Jason D. Fiedler, Guangmin Xia, Justin D. Faris, Yong‑Qiang Gu, Upinder Gill, Xiwen Cai, Maricelis Acevedo, Steven S. Xu 2022 USDA-ARS

Genome‑Wide Association Analyses Of Leaf Rust Resistance In Cultivated Emmer Wheat, Dhondup Lhamo, Qun Sun, Qijun Zhang, Xuehui Li, Jason D. Fiedler, Guangmin Xia, Justin D. Faris, Yong‑Qiang Gu, Upinder Gill, Xiwen Cai, Maricelis Acevedo, Steven S. Xu

Department of Agronomy and Horticulture: Faculty Publications

Leaf rust, caused by Puccinia triticina (Pt), constantly threatens durum (Triticum turgidum ssp. durum) and bread wheat (Triticum aestivum) production worldwide. A Pt race BBBQD detected in California in 2009 poses a potential threat to durum production in North America because resistance source to this race is rare in durum germplasm. To find new resistance sources, we assessed a panel of 180 cultivated emmer wheat (Triticum turgidum ssp. dicoccum) accessions for seedling resistance to BBBQD and for adult resistance to a mixture of durum-specific races BBBQJ, CCMSS, and MCDSS in the field, and genotyped …


Nebline, November/December 2022, 2022 University of Nebraska - Lincoln

Nebline, November/December 2022

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Holiday Wellness Tips by Alyssa Havlovic

Holiday Food Safety Quiz by Alyssa Havlovic

Recipe of the Month: Acorn Squash Cranberry Kale Salad by Alyssa Havlovic

Winter Protection for Potted Trees and Shrubs by Sarah Browning

Garden Guide: Things to Do This Month

What's the Deal With Smelly Well Water? by Becky Schuerman

Cover Crop Grazing Conference, Nov. 1

Pantry Pests by Kait Chapman

Bedtime Blues for Young Children by Hayley Jackson

Heart of 4-H Volunteer Award: Beatrice 77 Livestock and Lon and Carol Bohling

State 4-H Archery Outdoor Results

20 Years of Heart of 4-H Award!

4-H Announcements for Enrolled …


Near-Atomic, Non-Icosahedrally Averaged Structure Of Giant Virus Paramecium Bursaria Chlorella Virus 1, Qianqian Shao, Irina V. Agarkova, Eric A. Noel, David D. Dunigan, Yunshu Liu, Aohan Wang, Mingcheng Guo, Linlin Xie, Xinyue Zhao, Michael G. Rossmann, James L. Van Etten, Thomas Klose, Qianglin Fang 2022 Sun Yat-sen University

Near-Atomic, Non-Icosahedrally Averaged Structure Of Giant Virus Paramecium Bursaria Chlorella Virus 1, Qianqian Shao, Irina V. Agarkova, Eric A. Noel, David D. Dunigan, Yunshu Liu, Aohan Wang, Mingcheng Guo, Linlin Xie, Xinyue Zhao, Michael G. Rossmann, James L. Van Etten, Thomas Klose, Qianglin Fang

Department of Plant Pathology: Faculty Publications

Giant viruses are a large group of viruses that infect many eukaryotes. Although components that do not obey the overall icosahedral symmetry of their capsids have been observed and found to play critical roles in the viral life cycles, identities and high-resolution structures of these components remain unknown. Here, by determining a near-atomic-resolution, five-fold averaged structure of Parameciumbursaria chlorella virus 1, we unexpectedly found the viral capsid possesses up to five major capsid protein variants and a penton protein variant. These variants create varied capsidmicroenvironments for the associations of fibers, a vesicle, and previously unresolved minor capsid proteins. Our structure …


Evidence For Phylogenetic Signal And Correlated Evolution In Plant-Water Relations Traits, Eleinis Ávila-Lovera, Klaus Winter, Gregory R. Goldsmith 2022 Chapman University

Evidence For Phylogenetic Signal And Correlated Evolution In Plant-Water Relations Traits, Eleinis Ávila-Lovera, Klaus Winter, Gregory R. Goldsmith

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Evolutionary relationships are likely to play a significant role in shaping plant physiological and structural traits observed in contemporary taxa. We review research on phylogenetic signal and correlated evolution in plant-water relations traits, which play important roles in allowing plants to acquire, use and conserve water. We found more evidence for a phylogenetic signal in structural traits (e.g., stomatal length, stomatal density) than in physiological traits (e.g., stomatal conductance, water potential at turgor loss). Although water potential at turgor loss is the most-studied plant-water relations trait in an evolutionary context, it is the only trait consistently found to not have …


Salt Tolerance Qtls Of An Endemic Rice Landrace, Horkuch At Seedling And Reproductive Stages, Taslima Haque, Sabrina M. Elias, Samsad Razzaque, Sudip Biswas, Sumaiya Farah Khan, G. M. Nurnabi Azad Jewel, Md. Sazzadur Rahman, Thomas E. Juenger, Zeba I. Seraj 2022 University of Dhaka, University of Texas

Salt Tolerance Qtls Of An Endemic Rice Landrace, Horkuch At Seedling And Reproductive Stages, Taslima Haque, Sabrina M. Elias, Samsad Razzaque, Sudip Biswas, Sumaiya Farah Khan, G. M. Nurnabi Azad Jewel, Md. Sazzadur Rahman, Thomas E. Juenger, Zeba I. Seraj

Department of Agronomy and Horticulture: Faculty Publications

Salinity has a significant negative impact on production of rice. To cope with the increased soil salinity due to climate change, we need to develop salt tolerant rice varieties that can maintain their high yield. Rice landraces indigenous to coastal Bangladesh can be a great resource to study the genetic basis of salt adaptation. In this study, we implemented a QTL analysis framework with a reciprocal mapping population developed from a salt tolerant landrace Horkuch and a high yielding rice variety IR29. Our aim was to detect genetic loci that contributes to the salt adaptive responses of the two …


Rice Chalky Grain 5 Regulates Natural Variation For Grain Quality Under Heat Stress, Anil Kumar Nalini Chandran, Jaspreet Sandhu, Larissa Irvin, Puneet Paul, Balpreet K. Dhatt, Waseem Hussain, Tian Gao, Paul E. Staswick, Hongfeng Yu, Gota Morota, Harkamal Walia 2022 University of Nebraska-Lincoln

Rice Chalky Grain 5 Regulates Natural Variation For Grain Quality Under Heat Stress, Anil Kumar Nalini Chandran, Jaspreet Sandhu, Larissa Irvin, Puneet Paul, Balpreet K. Dhatt, Waseem Hussain, Tian Gao, Paul E. Staswick, Hongfeng Yu, Gota Morota, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Heat stress occurring during rice (Oryza sativa) grain development reduces grain quality, which often manifests as increased grain chalkiness. Although the impact of heat stress on grain yield is well-studied, the genetic basis of rice grain quality under heat stress is less explored as quantifying grain quality is less tractable than grain yield. To address this, we used an image-based colorimetric assay (Red, R; and Green, G) for genome-wide association analysis to identify genetic loci underlying the phenotypic variation in rice grains exposed to heat stress. We found the R to G pixel ratio (RG) derived from mature …


Alfalfa Water Productivity And Yield Gaps In The U.S. Central Great Plains, Kaylin P. Fink, Patricio Grassini, Alexandre Rocateli, Leonardo M. Bastos, Jude Kastens, Luke P. Ryan, Xiaomao Lin, Andres Patrignani, Romulo P. Lollato 2022 Kansas State University

Alfalfa Water Productivity And Yield Gaps In The U.S. Central Great Plains, Kaylin P. Fink, Patricio Grassini, Alexandre Rocateli, Leonardo M. Bastos, Jude Kastens, Luke P. Ryan, Xiaomao Lin, Andres Patrignani, Romulo P. Lollato

Department of Agronomy and Horticulture: Faculty Publications

Context: Yield gap (Yg) analyses using farmer-reported yield and management data have been performed for a number of annual grain crops, but it lacks for perennial forages. The U.S. accounts for 21 % of the global alfalfa production with a large rainfed area located in the central Great Plains, serving as an interesting case-study for Yg in perennial forages. Most existing alfalfa Yg analyses quantified the magnitude of the Yg but failed to identify associated management practices to reduce it. Challenging this analysis, a systematic benchmark for alfalfa water productivity [WP, kg dry matter per mm evapotranspiration (ETc)] that …


Effect Of Water Stress On Weed Germination, Growth Characteristics, And Seed Production: A Global Meta-Analysis, Mandeep Singh, Resham Thapa, Meetpal Singh Kukal, Suat Irmak, Steven Mirsky, Amit J. Jhala 2022 University of Nebraska-Lincoln

Effect Of Water Stress On Weed Germination, Growth Characteristics, And Seed Production: A Global Meta-Analysis, Mandeep Singh, Resham Thapa, Meetpal Singh Kukal, Suat Irmak, Steven Mirsky, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Weeds compete with crops for soil moisture, along with other resources, which can impact the germination, growth, and seed production of weeds; however, this impact has not been systematically recorded and synthesized across diverse studies. To address this knowledge gap, a global meta-analysis was conducted using 1,196 paired observations from 86 published articles assessing the effect of water stress on weed germination, growth characteristics, and seed production. These studies were conducted and published during 1970 through 2020 across four continents (Asia, Australia, Europe, and North America). Imposed water stress was expressed as solution osmotic potential (ψsolution), soil water potential (ψsoil), …


Agronomic And Quality Impact Of A Shortened Translocation For Wheat Streak Mosaic Virus Resistance, Mary J. Guttieri, Robert L. Bowden, Guorong Zhang, Scott Haley, Katherine Anna Frels, Gary L. Hein, Katherine W. Jordan 2022 USDA-ARS

Agronomic And Quality Impact Of A Shortened Translocation For Wheat Streak Mosaic Virus Resistance, Mary J. Guttieri, Robert L. Bowden, Guorong Zhang, Scott Haley, Katherine Anna Frels, Gary L. Hein, Katherine W. Jordan

Department of Agronomy and Horticulture: Faculty Publications

Wheat streak mosaic virus (WSMV) is a mite-vectored virus with substantial economic impact on wheat production. One of the effective sources of resistance to WSMV, Wsm1, is carried on a translocation from Thinopyrum intermedium. The original whole arm form of this translocation (T4DL·4JsS) was highly effective against WSMV but carried a substantial yield penalty in the absence of the virus. Shorter recombinants of the Th. intermedium translocation are now available. This study characterized the agronomic performance of near-isogenic sib-pair families in 10 yield trials using one of these shortened T4DL·4DS-4JsS translocations, named “rec213,” in the absence …


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