Other Plant Sciences Commons^™

A Phytobacterial Tir Domain Effector Manipulates Nad+ To Promote Virulence, Samuel Eastman, Thomas Smith, Mark A. Zaydman, Panya Kim, Samuel Martinez, Neha Damaraju, Aaron Diantonio, Jeffrey Milbrandt, Thomas Clemente, James R. Alfano, Ming Guo

Department of Agronomy and Horticulture: Faculty Publications

The Pseudomonas syringae DC3000 type III effector HopAM1 suppresses plant immunity and contains a Toll/interleukin-1 receptor (TIR) domain homologous to immunity-related TIR domains of plant nucleotide-binding leucine-rich repeat receptors that hydrolyze nicotinamide adenine dinucleotide (NAD+) and activate immunity. In vitro and in vivo assays were conducted to determine if HopAM1 hydrolyzes NAD+ and if the activity is essential for HopAM1’s suppression of plant immunity and contribution to virulence. HPLC and LC-MS were utilized to analyze metabolites produced from NAD+ by HopAM1 in vitro and in both yeast and plants. Agrobacterium-mediated transient expression and in planta inoculation assays were performed to …

Predicting Munsell Color For Turfgrass Leaves, William L. Berndt, Roch E. Gaussoin

Department of Agronomy and Horticulture: Faculty Publications

Linking turfgrass color to hue, value, chroma (H V/C) in the Munsell Plant Tissue Color Book is a visual comparison process for specifying and communicating plant color. If subjectivity of visual comparison can be mitigated, then accuracy of color matching may be improved. Research was conducted to develop an algorithm predicting H V/C from CIE-xyY color (xyY) in digital images of leaves of four turfgrasses. First, value-chroma (V/C) arrays for Munsell hue groups 5Y, 2.5GY, 5GY, 7.5GY, 10GY, and 2.5G were converted to xyY. Next, chromaticity (xy) plots from each array were fitted with …

Regenerating Agroecosystems By Overcoming Human Exceptionalism In Designing For Increased Equity Of Benefits From Ecoservices, Ali Loker, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Our commentary explores three critical issues related to ecosystem services. First is how ecoservices are currently designed and implemented primarily for human benefit without concern for how these impact other species. We conclude that awareness of this imbalance is the first step toward meaningful change. Second we observe that human exceptionalism guides most decisions, and ask whether we can overcome this mind-set to embrace ecoregeneration and design of resilient and mutually beneficial agroecosystems. Our attitude toward the challenge and moving toward greater humility about human roles that guide management decisions in the ecosystem is a requisite for change. Third we …

Working Tree Inventory, Steven Sypniewski

Maud Gordon Holmes Arboretum database

Inventory of trees on the Buffalo State Campus starting in 2019.

Botanicals As Prospective Agents Against Sars-Cov-2 Virus, Vyacheslav Dushenkov, Anna Dushenkov

Publications and Research

The COVID-19 high morbidity and mortality resulted in a worldwide healthcare emergency that triggered accelerated and amplified efforts in the relevant areas of health sciences research and practice. Purpose: To assess the potential role of botanicals as therapeutic agents against the SARS-CoV-2 virus. Methods: This narrative review examined the potential role of botanicals as therapeutic agents against the SARS-CoV-2 virus based on the references limited to the English language and published up to February 2022 and retrieved from common academic search engines using multiple keywords and their combinations. The scientific names of plant species were confirmed using World Flora Online …

Role Of Seed Mixes, Biochar Soil Amendment, And The Soil Seed Bank In Improving Palouse Prairie Restoration, Kristine Snyder

EWU Masters Thesis Collection

The Palouse Prairie Ecoregion of Eastern Washington and Idaho is critically endangered. Eastern Washington University (EWU) has begun a ~120 acre campus prairie restoration; however, there is considerable uncertainty about the most effective restoration approach. Annual seeds and soil organic matter are important for succession and soil moisture, but their roles are unknown for Palouse prairie restoration. Additionally, the soil seedbank of the Palouse ecoregion is widely unknown but could provide insight into methods or species to use for restoration. My research aimed to determine the effects of seed mix diversity, annual seed addition, and soil organic matter (as biochar) …

Field Validation Of A Farmer Supplied Data Approach To Close Soybean Yield Gaps In The Us North Central Region, José F. Andrade, Spyridon Mourtzinis, Juan I. Rattalino Edreira, Shawn P. Conley, John Gaska, Herman J. Kandel, Laura E. Lindsey, Seth Naeve, Scott Nelson, Maninder P. Singhi, Laura J. Thompson, James E. Specht, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

CONTEXT: Producer-reported data can be used to identify suites of management practices that lead to higher yield and profit. However, a rigorous validation of the approach in relation to its potential impact on farmer yield and profit is lacking.

OBJECTIVE: This study aimed to validate a producer-data approach on its capability to guide on-farm evaluation of management practices with greatest potential for increasing producer yield and profit. We show proof of concept using soybean in the North Central US region as a case study.

METHODS: We used a combination of regression tree analysis and a spatial framework to determine practices …

Ecological Intensification With Soil Health Practices Demonstrates Positive Impacts On Multiple Soil Properties: A Large-Scale Farmer-Led Experiment, Fernanda Souza Krupek, Daren D. Redfearn, Kent M. Eskridge, Andrea Basche

Department of Agronomy and Horticulture: Faculty Publications

Improving soil health is critical to reversing trends of soil degradation and is of increasing interest to a range of stakeholders including policymakers, agricultural industry leaders, food companies, and farmers. Crop and soil management practices focused on ecological functions can be effective in restoring fundamental biological, chemical and physical soil properties. The call for ecological intensification of agricultural systems has the potential to improve soil health and input-use efficiency. In this study, we developed a framework to classify spatial and temporal ecological intensification with soil health practices: tillage, crop rotation, cover crop, organic amendment, and crop-livestock integration. We applied this …

Getting To The Root Cause: The Genetic Underpinnings Of Root System Architecture And Rhizodeposition In Sorghum, Farren Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Plants are some of the most diverse organisms on earth, consisting of more than 350,000 different species. To understand the underlying processes that contributed to plant diversification, it is fundamental to identify the genetic and genomic components that facilitated various adaptations over evolutionary history. Most studies to date have focused on the underlying controls of above-ground traits such as grain and vegetation; however, little is known about the “hidden half” of plants. Root systems comprise half of the total plant structure and provide vital functions such as anchorage, resource acquisition, and storage of energy reserves. The execution of these key …

The Impacts Of Maple Syrup Production On The Herbaceous Layer In Appalachian Hardwood Forests, Logan Scott Ferguson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Vegetation data were collected from eight maple syrup farms (sugarbushes) and eight undeveloped maple-dominated sites to examine potential differences in understory plant communities due to disturbance effects. Understory plants were identified to the species level and percent cover of aboveground leaf-area for each species was estimated within 600 total quadrats. Overstory data and environmental data were collected to help determine if they had any effect on herbaceous-layer plant communities. Species richness, Shannon diversity, and Pielou’s evenness were calculated, with the analysis showing no significant differences between the site types. The absolute cover of plant functional groups was also compared between …

Charles A. Francis, Abstracts And Publications [1969--2022], December 2022, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Contents

I. Journal articles (218), p.1

II. Book reviews (60), p. 18

III. Published abstracts (213), p. 23

IV. Books (23), p. 40

V. Chapters in books (81), p. 42

VI. Symposium or workshop proceedings (127), p. 50

VII. Symposia or extension publications edited (31), p. 62

VIII. Miscellaneous (522), p. 65

Improving Taxonomic Delimitation Of Fungal Species In The Age Of Genomics And Phenomics, Ashley Stengel, Kimberly M. Stanke, Amanda C. Quattrone, Joshua R. Herr

Department of Plant Pathology: Faculty Publications

Species concepts have long provided a source of debate among biologists. These lively debates have been important for reaching consensus on how to communicate across scientific disciplines and for advancing innovative strategies to study evolution, population biology, ecology, natural history, and disease epidemiology. Species concepts are also important for evaluating variability and diversity among communities, understanding biogeographical distributions, and identifying causal agents of disease across animal and plant hosts. While there have been many attempts to address the concept of species in the fungi, there are several concepts that have made taxonomic delimitation especially challenging. In this review we discuss …

Determining The Novel Pathogen Neodothiora Populina As The Causal Agent Of The Aspen Running Canker Disease In Alaska, Loretta M. Winton, Gerard C. Adams, Roger W. Ruess

Department of Plant Pathology: Faculty Publications

Neodothiora populina Crous, G.C. Adams & Winton was determined to be a new pathogen of trembling aspen (Populus tremuloides) growing in Alaska, based on completion of Koch's Postulates in replicated forest and growth chamber inoculation trials. The pathogen is responsible for severe damage and widespread rapid mortality of sapling to mature aspen (≥ 80 years) in the boreal forests of interior Alaska, due to large diffuse annual (1–2 years) cankers. Isolation of the pathogen was challenging, and identification based on cultural characters was difficult. Fruiting bodies were not found on wild diseased trees, but erumpent pycnidia were found …

Identification Of Beneficial And Detrimental Bacteria Impacting Sorghum Responses To Drought Using Multi-Scale And Multisystem Microbiome Comparisons, Mingsheng Qi, Jeffrey C. Berry, Kira W. Veley, Lily O'Connor, Omri M. Finkel, Isai Salas-González, Molly Kuhs, Julietta Jupe, Emily Holcomb, Tijana Glavina Del Rio, Cody Creech, Peng Liu, Susannah G. Tringe, Jeffery L. Dangl, Daniel P. Schachtman, Rebecca S. Bart

Department of Agronomy and Horticulture: Faculty Publications

Drought is a major abiotic stress limiting agricultural productivity. Previous field-level experiments have demonstrated that drought decreases microbiome diversity in the root and rhizosphere. How these changes ultimately affect plant health remains elusive. Toward this end, we combined reductionist, transitional and ecological approaches, applied to the staple cereal crop sorghum to identify key root-associated microbes that robustly affect drought-stressed plant phenotypes. Fifty-three Arabidopsis-associated bacteria were applied to sorghum seeds and their effect on root growth was monitored. Two Arthrobacter strains caused root growth inhibition (RGI) in Arabidopsis and sorghum. In the context of synthetic communities, Variovorax strains were able to …

Temporal Changes In The Nutrient Content Of Cattle Dung In The Nebraska Sandhills Ecosystem, Amanda Shine, Martha Mamo, Gandura O. Abagandura, Walter Schacht, Jerry Volesky

Department of Agronomy and Horticulture: Faculty Publications

Dung excreted by cattle composes a significant portion of the nutrient inputs in a grazed ecosystem and can have wide-ranging effects on soil properties and vegetation. However, little research has been conducted on the nutrient dynamics of excreted dung in situ that has not been disturbed prior to field sampling. In this study, we analyzed 294 dung pats (1–24 days old) collected from a Nebraska Sandhills meadow to determine water-extractable organic carbon (WEOC), water-extractable nitrogen (WEN), water-extractable phosphorus (WEP), and percent dry matter (DM) changes over time. In addition, we investigated if sample handling - frozen storage – and the …

Common Garden Study Reveals Frost-Tolerant, Generalist Northern Seed Sources Are Best Suited To Expand Range Of Quercus Rubra, Emily Lindback

Dissertations, Master's Theses and Master's Reports

Climate change is shifting the potential suitable range of northern red oak (Quercus rubra L.) faster than it can migrate, creating an adaptation lag. Quercus rubra is prominent in eastern North American forests and important for its carbon sequestration, ecological roles, and economic significance. Our study addresses which populations of Q. rubra are best suited for climatic conditions at the northern and central range limits. Our findings can inform forestry management policies such as assisted migration, where species are transferred within their native range to mitigate the effects of climate change. We planted over 800 Q. rubra seedlings …

A New Perspective When Examining Maize Fertilizer Nitrogen Use Efficiency, Incrementally, Newell R. Kitchen, Curtis J. Ransom, James S. Schepers, Jerry L. Hatfield, Raymond Massey, Scott T. Drummond

Department of Agronomy and Horticulture: Faculty Publications

For maize (Zea mays L.), nitrogen (N) fertilizer use is often summarized from field to global scales using average N use efficiency (NUE). But expressing NUE as averages is misleading because grain increase to added N diminishes near optimal yield. Thus, environmental risks increase as economic benefits decrease. Here, we use empirical datasets obtained in North America of maize grain yield response to N fertilizer (n = 189) to create and interpret incremental NUE (iNUE), or the change in NUE with change in N fertilization. We show for those last units of N applied to reach economic optimal N …

First Plant Cell Atlas Symposium Report, Selena L. Rice, Elena Lazarus, Christopher Anderton, Kenneth Birnbaum, Jenniofer Brophy, Benjamin Cole, Diane Dickel, David Ehrhardt, Noah Fahlgren, Margaret Frank, Elizabeth Haswell, Shao-Shan Carol Huang, Samuel Leiboff, Marc Libault, Marisa S. Otegui, Nicholas Provart, R. Glen Uhrig, Seung Y. Rhee, The Plant Cell Atlas Consortium

Department of Agronomy and Horticulture: Faculty Publications

The Plant Cell Atlas (PCA) community hosted a virtual symposium on December 9 and 10, 2021 on single cell and spatial omics technologies. The conference gathered almost 500 academic, industry, and government leaders to identify the needs and directions of the PCA community and to explore how establishing a data synthesis center would address these needs and accelerate progress. This report details the presentations and discussions focused on the possibility of a data synthesis center for a PCA and the expected impacts of such a center on advancing science and technology globally. Community discussions focused on topics such as data …

A Non-Destructive Approach For Measuring Rice Panicle-Level Photosynthetic Responses Using 3d-Image Reconstruction, Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Paul Staswick, Jason Hupp, Hongfeng Yu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Our understanding of the physiological response of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impact on grain yield. This is primarily due to lack of a suitable gas exchange methodology for panicles, as well as non-destructive methods to accurately determine panicle surface area. To address these challenges, we have developed a custom panicle gas exchange cylinder compatible with the LiCor 6800 Infra-red Gas Analyzer. Accurate surface area measurements were determined with a 3D panicle imaging platform to normalize the panicle-level photosynthetic measurements. We observed differential responses in both …

Inactivation Of The Entire Arabidopsis Group Ii Gh3s Confers Tolerance To Salinity And Water Deficit, Rubén Casanova-Sáez, Eduardo Mateo-Bonmatí, Jan Šimura, Aleš Pěnčík, Ondřej Novák, Paul Staswick, Karin Ljung

Department of Agronomy and Horticulture: Faculty Publications

Indole-3-acetic acid (IAA) controls a plethora of developmental processes. Thus, regulation of its concentration is of great relevance for plant performance. Cellular IAA concentration depends on its transport, biosynthesis and the various pathways for IAA inactivation, including oxidation and conjugation.

Group II members of the GRETCHEN HAGEN 3 (GH3) gene family code for acyl acid amido synthetases catalysing the conjugation of IAA to amino acids. However, the high degree of functional redundancy among them has hampered thorough analysis of their roles in plant development.

In this work, we generated an Arabidopsis gh3.1,2,3,4,5,6,9,17 (gh3oct) mutant to knock out the group II …