Innovations In Plant Genome Editing And Biotechnology: Addressing Global Agricultural Challenges,
2024
TÜBİTAK
Innovations In Plant Genome Editing And Biotechnology: Addressing Global Agricultural Challenges, Bayram Ali̇ Yerli̇kaya, Karam Mostafa, Abdullah Aydin, Seher Yerli̇kaya, Momna Mehmood, Ni̇sa Nur Yilmaz, Kubi̇lay Yildirim, Musa Kavas
Turkish Journal of Botany
Developments in genome editing and plant biotechnology in recent years, especially CRISPR-Cas9 and RNA interference (RNAi) technologies, offer essential tools for solving global agricultural problems that are worsened by climate change and the increasing food demand caused by the rising world population. This review focuses on the urgent need to feed a growing global population. It explores how key biotechnological tools can enhance crop resilience and nutritional quality amid climate variability and extreme weather. The applicability of CRISPR-Cas technologies in the development of climate-friendly, more nutritious, and higher-yielding crops has opened a biotechnological avenue for the development of both transgenic …
Advancements In Qtl Mapping And Gwas Application In Plant Improvement,
2024
TÜBİTAK
Advancements In Qtl Mapping And Gwas Application In Plant Improvement, Muhammad Tanveer Altaf, Muhammed Tatar, Amjad Ali, Waqas Liaqat, Parnaz Mortazvi, Ceyhun Kayihan, Fati̇h Ölmez, Muhammad Azhar Nadeem, Jazib Javed, Jin-Ying Gou, Meng-Lu Wang, Ummad Ud Din Umar, Hayri̇ye Yildiz Daşgan, Cemal Kurt, Mehtap Yildiz, Sheikh Mansoor, Abdelfattah A. Dababat, Nafiz Çeli̇ktaş, Faheem Shehzad Baloch
Turkish Journal of Botany
Molecular markers play a crucial role in modern plant breeding by enabling the precise identification of genetic loci associated with important agronomic traits. This article focuses on the advancements in Quantitative Trait Locus (QTL) mapping and Genome-Wide Association Studies (GWAS), which are pivotal in identifying and dissecting complex traits such as stress tolerance, yield improvement, and nutrient efficiency. Through QTL mapping, significant genetic regions linked to desired traits are identified, while GWAS leverages larger populations for higher resolution in genetic association studies. The integration of next-generation sequencing, CRISPR, and transcriptomics technologies has revolutionized the precision and efficiency of these methods, …
Identification Of Genes Associated With Zinc And Copper Concentrations In Walnut (Juglans Regia L.) By Gwas Analysis,
2024
TÜBİTAK
Identification Of Genes Associated With Zinc And Copper Concentrations In Walnut (Juglans Regia L.) By Gwas Analysis, Berfi̇n Nazlican Kaman, Bülent Yağmur, Erdal Orman, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş
Turkish Journal of Botany
154 walnut genotypes from various geographic regions were analyzed in the study. Micronutrient concentrations of zinc (Zn) ranged from 1.61 to 8.98 mg/100 g, and the concentrations of copper (Cu) varied between 0.12 and 0.38 mg/100 g. 16,473 SNPs were generated using Diversity Array Technology (DArT) for genome-wide association (GWAS) studies. The Mixed Linear Model (MLM) utilizing the Q + K model revealed seven associated markers for the element Zn in 2021 and four in 2022. The MLM (Q + K) model indicated two associated markers for the element Cu in 2021 and three in 2022. The markers found in …
Genome-Wide Identification And Characterization Of The Lea Gene Family In Common Bean And The Role Of The Dehydrin Gene Pvhdn3 In Enhancing Salt And Drought Tolerance In Transgenic Tobacco Plant., Gökhan Gökdemi̇r, Zafer Seçgin, Karam Mostafa, Mohamed Farah Abdulla, Musa Kavas
Turkish Journal of Botany
Global climate change exposes crops to numerous abiotic and biotic stress factors, significantly threatening their growth, development, and overall productivity. Among the various abiotic stresses, salt and drought are the most detrimental, having a severe impact on yield stability. Late Embryogenesis Abundant proteins (LEA) represent a broad and varied class of polypeptides that accumulate in response to cellular dehydration across diverse organisms. Nonetheless, there has been a scarcity of studies on this protein family in common beans prior to this investigation. In this study, we identified 69 members of the LEA gene family in common bean, characterized by conserved LEA …
Gene Expression Changes In The Seagrass Cymodocea Nodosa Individuals In Response To Aquatic Acidification,
2024
Ege University
Gene Expression Changes In The Seagrass Cymodocea Nodosa Individuals In Response To Aquatic Acidification, Enes Göksal, Esra Öztürk Yi̇ği̇t
Turkish Journal of Botany
Human activities have caused a rise in atmospheric carbon dioxide (CO2) levels, leading to greater absorption of CO2 by oceans and causing ocean acidification. This phenomenon, marked by a reduction in pH, represents substantial risks to marine ecosystems, including seagrass meadows. Seagrasses are vital elements of coastal ecosystems, performing important functions in carbon storage, stabilizing shorelines, and preserving biodiversity; however, reactions to ocean acidification are not well understood, especially in terms of molecular.This research study examined alterations in gene expression within seagrass meadows, namely the species Cymodocea nodosa, in reaction to simulated ocean acidification conditions. Climate chamber system to adjust …
Phylogenomics Of The Tribe Leonureae Reveals A New Genus: Paraleonurus (Lamiaceae, Lamioideae),
2024
TÜBİTAK
Phylogenomics Of The Tribe Leonureae Reveals A New Genus: Paraleonurus (Lamiaceae, Lamioideae), Mei-Lin Qian, Ya-Ping Chen, Tuncay Di̇rmenci̇, Yue Zhao, Guo-Xiong Hu, Fei Zhao, Ferhat Celep, Bo Li, Chunlei Xiang
Turkish Journal of Botany
With approximately 80 species across six genera (Leonurus, Lagopsis, Panzerina, Lagochilus, Chaiturus, and Loxocalyx), the tribe Leonureae represents one of the most morphologically diverse tribes within the subfamily Lamioideae of Lamiaceae. Previous studies indicated that Leonurus and Lagopsis are not monophyletic, and relationships among and within these genera remain unclear due to limited sampling and DNA markers. In this study, we present the most comprehensive phylogenomic analyses of Leonureae based on complete plastome sequences and 127 nuclear loci retrieved from next-generation sequencing data. Our results provide strong evidence for the monophyly of Leonureae, which can be further divided into six …
Selective Indel Marker Identification Across The Peanut (Arachis Hypogaea L.) Genome Using Ddradseq,
2024
TÜBİTAK
Selective Indel Marker Identification Across The Peanut (Arachis Hypogaea L.) Genome Using Ddradseq, Moin Qureshi, Bi̇rgül Guden, Hi̇lal Şule Tosun, Mojtaba Kordrostami, Sunil Shiwaji Gangurde, Bülent Uzun, Engi̇n Yol
Turkish Journal of Botany
The advent of next-generation sequencing technologies, mainly double digest restriction site-associated DNA sequencing (ddRADSeq), has significantly advanced the development of molecular markers for crop genetics. This study used ddRADSeq to identify and develop insertion-deletion (InDel) markers in 25 peanut genotypes from diverse geographic regions. The bioinformatic analysis unveiled 62728 InDels across the peanut genome, predominantly between 1-5 bp, which constituted 96% of the total, while InDels of ≥ 6 bp accounted for 3.96%. We focused on 1013 InDels of at least 10 bp for further analysis, representing 1.61% of the total reads, with a distribution of 832 insertions and 181 …
Exploring The Population Structure And Genetic Diversity In Apple Germplasm Using Ipbs-Retrotransposon Markers,
2024
TÜBİTAK
Exploring The Population Structure And Genetic Diversity In Apple Germplasm Using Ipbs-Retrotransposon Markers, Aigul Madenova, Angsagan Kuan, Amjad Ali, Muhammad Azhar Nadeem, Muhammad Tanveer Altaf, Balnur Kabylbekova, Dinara Kaldybaeva, Ti̇mur Turdi̇yev, Faheem Shahzad Baloch
Turkish Journal of Botany
The apple (Malus domestica [Suckow] Borkh) is economically and culturally significant worldwide, thriving in temperate regions. This study examined genetic variations among 52 apple accessions from three research centers in Kazakhstan using the iPBS-retrotransposons marker system. Out of 35 screened markers, 12 highly polymorphic markers were selected for PCR amplification, resulting in 280 bands, 279 of which were polymorphic, yielding a 99.64% polymorphism rate. Genetic diversity indices showed substantial variation: effective number of alleles (ne=1.655), Shannon's information index (I=0.373), gene diversity (h=0.549), and an average genetic distance of 1.2. The analysis of molecular variance (AMOVA) indicated that 98.80% of the …
Heterologous Expression Of Haloarchaeal Transporter Genes In Nicotiana Tabacum Under Salt And Drought Stresses,
2024
TÜBİTAK
Heterologous Expression Of Haloarchaeal Transporter Genes In Nicotiana Tabacum Under Salt And Drought Stresses, Çi̇ğdem Otur, Musa Kavas, Aslihan Kurt Kizildoğan
Turkish Journal of Botany
The objective of the study: Climate change and human activities impose severe biotic and abiotic stresses that threaten plant survival. To cope with these extreme conditions, plants have evolved various resistance mechanisms. In recent years, genetic approaches have been increasingly employed to enhance abiotic stress tolerance and develop stress-resistant plants. In this study, we introduced three transporter-related genes from extreme haloarchaea, which thrive in saturated NaCl environments, into the Nicotiana tabacum genome to improve its salt and drought tolerance mechanisms.E xperimental procedure(s) : The U2845, U3508, and CL165 genes were subcloned into the pIPKb004 vector, which includes the cauliflower mosaic …
Role Of Sulfur Metabolism In Acquiring Of Boron Tolerance In Arabidopsis Thaliana,
2024
TÜBİTAK
Role Of Sulfur Metabolism In Acquiring Of Boron Tolerance In Arabidopsis Thaliana, Seda Yi̇rmi̇beş, Ceyhun Kayihan, Nuran Çi̇çek, Yasemi̇n Ekmekçi̇
Turkish Journal of Botany
The purpose of this study was to assess whether sulfate applications (pre- and combined) caused the changes of tolerance against boron (B) toxicity in Arabidopsis thaliana at physiological, biochemical and molecular levels. Plants were germinated and grown on MS medium for 12 days, and then, sulfate pre-treatment groups (PS-B) were transferred to sulfate-containing media for 3 days. Subsequently, PS-B, combined (S+B) and un-treated application groups were transferred to medium containing 3 and 5 mM B for 10 days. Leaf area, plant biomass and total chlorophyll content decreased significantly depending on the accumulation of B. Toxic levels of B adversely affected …
Roquea, A New Genus Of Lychnophorinae (Vernonieae, Asteraceae) From Brazil And Its Phylogenetic Placement,
2024
Royal Botanic Gardens, Kew
Roquea, A New Genus Of Lychnophorinae (Vernonieae, Asteraceae) From Brazil And Its Phylogenetic Placement, Benoit Loeuille, Roberto Baptista Pereira Almeida, Carolina Moriani Siniscalchi, Makeli G. Lusa, Guilherme Medeiros Antar
UL Publications
Lychnophorinae (Vernonieae, Asteraceae) consists of 21 genera and approximately 150 species, primarily distributed in the Brazilian Central Plateau, with several novelties recently described. With new botanical expeditions conducted in Serra da Formosa, state of Minas Gerais, a set of collections that belong to Lychnophorinae but were not easily assigned to a genus was discovered. After morphological and anatomical analyses and phylogenetic studies, we describe and illustrate Roquea, a new genus of Asteraceae from Brazil, to accommodate the new species Roquea multiserialis. Affinities of the new genus with other genera of Lychnophorinae are discussed along with a preliminary conservation …
Leveraging Within-Field Spatial Variability To Optimize Soybean Yield, Seed Quality, And Profitability,
2024
University of Nebraska-Lincoln
Leveraging Within-Field Spatial Variability To Optimize Soybean Yield, Seed Quality, And Profitability, Marina Marly Dalla Betta
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Optimizing yield and seed quality in soybean production is critical for both profitability and meeting market demands. However, within-field variability in soil properties poses a challenge in determining optimal management strategies to maximize soybean yield, seed quality, and profitability. This work aimed to evaluate the spatial variability of soybean fields to optimize soybean seeding rates, seed quality, and profitability. Two case studies were used to carry over this research. The first study looked at the optimal economical site-specific soybean seeding rates using 11 on-farm precision experiments across Nebraska. The second study looked at the protein and oil variability within 22 …
Induced Systemic Resistance Against Nematodes By Plant Growth-Promoting Microbes In Potatoes,
2024
University of Northern Colorado
Induced Systemic Resistance Against Nematodes By Plant Growth-Promoting Microbes In Potatoes, Cole Grannan
Master's Theses
Biofertilizers consist of plant growth-promoting microorganisms that establish symbiotic relationships with plant roots and stems. These beneficial microbes play a crucial role in enhancing plant defenses against various threats including insects, nematodes, and microbial pathogens. The purpose of this study was to investigate the effectiveness of two fungal biofertilizers (an endophytic strain of Beauveria bassiana (BA) and a mixture of arbuscular mycorrhizal (AM) fungi), specifically their ability to induce systemic resistance against plant- parasitic nematodes (PPNs) in potatoes (Solanum tuberosum cv. Masquerade) under controlled greenhouse conditions. The study utilized soil collected from Colorado State University’s San Luis Valley (SLV) Research …
Evaluating The Duckweeds Lemna Minor And Spirodela Polyrhiza As Alternative Aquatic Hosts For The Spread Of The Bacterial Wilt Pathogen, Ralstonia Solanacearum,
2024
University of South Alabama
Evaluating The Duckweeds Lemna Minor And Spirodela Polyrhiza As Alternative Aquatic Hosts For The Spread Of The Bacterial Wilt Pathogen, Ralstonia Solanacearum, Irelynn Smith
Honors Theses
Ralstonia solanacearum is a plant pathogen that causes bacterial wilt disease in major crops such as tomatoes and potatoes. Recently, this bacterium was discovered in floating pennywort (Hydrocotyle ranunculoides) and Pennsylvania smartweed (Polygonum pennysylvanicum), two previously unsuspected aquatic plants. Duckweed is an aquatic plant with a high level of resilience and short doubling time. Common duckweed (Lemna minor) has previously demonstrated its capability as a versatile high-yield infection model for other types of pathogenic bacteria. Given this newfound branch of aquatic hosts and the fact that duckweed is a common pond weed previously identified in several ponds located nearby infected …
Evaluating The Roles Of Pollinators In Shaping The Floral Color Variation Of Phacelia Dubia,
2024
Clemson University
Evaluating The Roles Of Pollinators In Shaping The Floral Color Variation Of Phacelia Dubia, Katherine Riddle
All Theses
Floral color variation within a species is a common occurrence and its maintenance has been a long-standing question in evolutionary biology. Differences in flower color between populations can be shaped by pollinator-mediated selection. Pollinators can exhibit innate and learned preferences for color that may influence floral color variation. These preferences may be molded by the visual environment in which pollinators forage. Most current understanding of how pollinators shape floral color variation involves dramatic differences in dominant pollinators between populations (e.g., hawkmoth vs. bee) and most behavioral work utilized model pollinators like honeybee and bumble bee. However, less is known about …
Elevation And Population Size Effects On The Survival Of Drosera Rotundifolia,
2024
East Tennessee State University
Elevation And Population Size Effects On The Survival Of Drosera Rotundifolia, Jessica Williams
Electronic Theses and Dissertations
Carnivorous plants face significant conservation challenges due to habitat destruction, climate change, and illegal harvesting. This study explores how elevation and population size influence key-life history traits of D. rotundifolia, such as flowering phenology, prey capture efficiency, seed production, and germination rates. Higher elevations and larger populations are associated with extended flowering durations and increased prey capture. However, seed production and germination rates show complex patterns that are likely influenced by genetic diversity, resource availability, or environmental conditions. These insights inform targeted conservation strategies, emphasizing habitat preservation, enhancing prey availability, and maintaining genetic diversity. Public education and community engagement …
Broad-Spectrum Resistance To
Fungal Foliar Diseases In Wheat:
Recent Efforts And Achievements,
2024
Leibniz Institute of Plant Genetics and Crop Plant Research, Assuit University
Broad-Spectrum Resistance To Fungal Foliar Diseases In Wheat: Recent Efforts And Achievements, Amira M.I. Mourad, Asmaa A.M. Ahmed, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam
Department of Agronomy and Horticulture: Faculty Publications
Wheat (Triticum spp.) is one of the most important cereal crops in the world. Several diseases affect wheat production and can cause 20-80% yield loss annually. Out of these diseases, stripe rust, also known as yellow rust (Puccinia striiformis f. sp. tritici), stem rust (Puccinia graminis f. sp. tritici), leaf rust (Puccinia recondita), and powdery mildew (Blumeria graminis f. sp. tritici) are the most important fungal diseases that infect the foliar part of the plant. Many efforts were made to improve wheat resistance to these diseases. Due to the continuous advancement in …
Disentangling Genotype
And Environment Specific
Latent Features For Improved
Trait Prediction Using A
Compositional Autoencoder,
2024
Iowa State University
Disentangling Genotype And Environment Specific Latent Features For Improved Trait Prediction Using A Compositional Autoencoder, Anirudha Powadi, Talukder Zaki Jubery, Michael C. Tross, James C. Schnable, Baskar Ganapathysubramanian
Department of Agronomy and Horticulture: Faculty Publications
In plant breeding and genetics, predictive models traditionally rely on compact representations of high-dimensional data, often using methods like Principal Component Analysis (PCA) and, more recently, Autoencoders (AE). However, these methods do not separate genotype-specific and environment-specific features, limiting their ability to accurately predict traits influenced by both genetic and environmental factors. We hypothesize that disentangling these representations into genotype-specific and environment-specific components can enhance predictive models. To test this, we developed a compositional autoencoder (CAE) that decomposes high-dimensional data into distinct genotype-specific and environment-specific latent features. Our CAE framework employed a hierarchical architecture within an autoencoder to effectively separate …
Industrial Hemp Biomass Negatively
Affected By Herbicide Drift From
Corn And Soybean Herbicides,
2024
University of Nebraska-Lincoln
Industrial Hemp Biomass Negatively Affected By Herbicide Drift From Corn And Soybean Herbicides, Milos Zaric, Bruno Canella Vieira, Barbara Houston, Guilherme Sousa Alves, Samuel E. Wortman, Julie A. Peterson, Greg R. Kruger
Department of Agronomy and Horticulture: Faculty Publications
The establishment of industrial hemp (Cannabis sativa L.) fields near row crops has raised concerns about the potential adverse effects of herbicide drift on hemp production. This study examined hemp susceptibility to drift of herbicides registered for use in corn and/or soybeans. Herbicide solutions (2,4-D, dicamba, glufosinate, glyphosate, imazethapyr, lactofen, mesotrione) were applied separately in the wind tunnel (3.6 m s−1 airspeed), simulating drift scenarios, with conventional TP95015EVS (TP) and air inclusion AI95015EVS (AI) flat fan nozzles calibrated to deliver 140 L ha−1 carrier volume at 230 kPa. Mylar cards and hemp plants (20–25 cm tall) were placed …
Oregon State Rank Assessment For Pt. Reyes Bird's-Beak (Chloropyron Maritinum Ssp. Palustre),
2024
Portland State University
Oregon State Rank Assessment For Pt. Reyes Bird's-Beak (Chloropyron Maritinum Ssp. Palustre), Nora Dunkirk, Gabriel E. Campbell
Institute for Natural Resources Publications
Oregon state conservation status assessment for Pt. Reyes bird's-beak (Chloropyron maritimum ssp. palustre) using NatureServe methodology, 2024.
