The Effects Of Calcium Availability On Growth And Calcium Content Of Hydroponic Daikon Radish Microgreens,
2025
Missouri State University
The Effects Of Calcium Availability On Growth And Calcium Content Of Hydroponic Daikon Radish Microgreens, Cory Michael Reichmuth
Graduate Theses/Dissertations
Microgreens are young, edible seedlings harvested upon emergence of the first true leaf. Their popularity is growing as a nutritionally dense food source that can be grown quickly in space-efficient systems. However, little is known about optimal cultivation practices and the effects of fertilization on growth and nutrition of microgreens. This study investigates the role of calcium (Ca) fertilization in optimizing the growth, yield, and nutrient content of daikon radish (Raphanus sativus) microgreens. A hydroponic system was designed to consistently deliver nutrient solution to microgreens grown in a closed-environment chamber. This study tested different Ca fertilization rates of 0, 5, …
Responses Of Native And Invasive Aquatic Vegetation To Changes In Water Depth In An Ozark Spring-Fed Pond,
2025
Missouri State University
Responses Of Native And Invasive Aquatic Vegetation To Changes In Water Depth In An Ozark Spring-Fed Pond, Daphne Miles
Graduate Theses/Dissertations
Spring-fed aquatic ecosystems in the Ozark Highlands are characterized by stable water depths, clear water, and diverse aquatic vegetation (filamentous algae, submerged, floating, and emergent plants). Sudden changes in water depth can impact aquatic vegetation dynamics but the impacts of prolonged changes are poorly understood. I investigated seasonal and annual changes to aquatic vegetation abundance in a spring impoundment during and after a two-year period of low water depth that was caused by a broken outlet below an artificial dam. Water depth was significantly lower in 2020 and 2021 (low water years), compared to 2022 and 2023 (high water years). …
Best Management Practices: Sambucus Canadensis Propagation Techniques,
2025
Missouri State University
Best Management Practices: Sambucus Canadensis Propagation Techniques, Hannah Berendzen
Graduate Theses/Dissertations
American elderberry (Sambucus nigra ssp. canadensis) is a fruit-bearing shrub native to North America, traditionally utilized for medicinal and culinary purposes. In recent years, its popularity has surged due to emerging research supporting its immune-boosting properties. Despite its growing demand, standardized best management practices for elderberry cultivation, particularly propagation techniques, remain lacking. This study aims to clarify propagation recommendations by examining the influence cutting size and stem diameter on rooting success. A field study was conducted from February to May 2024, in which one-node and two-node dormant hardwood elderberry cuttings of varying stem diameters were planted in small …
ผลของสารสกัดหยาบจากเปลือกของประดู่ป่า Pterocarpus Macrocarpus Kurz ต่อฤทธิ์การต้านอนุมูลอิสระและความมีชีวิตของเซลล์มะเร็งลำไส้ใหญ่,
2025
คณะวิทยาศาสตร์
ผลของสารสกัดหยาบจากเปลือกของประดู่ป่า Pterocarpus Macrocarpus Kurz ต่อฤทธิ์การต้านอนุมูลอิสระและความมีชีวิตของเซลล์มะเร็งลำไส้ใหญ่, ฐิติกานต์ เกตุทอง
Chulalongkorn University Theses and Dissertations (Chula ETD)
มะเร็งลำไส้ใหญ่เป็นหนึ่งในสาเหตุหลักของการเสียชีวิตและการเจ็บป่วยทั่วโลก มีปัจจัยเสี่ยงหลายประการที่ส่งผลต่อมะเร็งลำไส้ใหญ่ หนึ่งในนั้นคือการเปลี่ยนแปลงระดับอนุมูลอิสระของออกซิเจน แม้ว่าการรักษาด้วยเคมีบำบัดจะมีประสิทธิภาพ แต่อัตราความสำเร็จก็ยังค่อนข้างต่ำเนื่องจากเกิดผลข้างเคียงด้านลบหลังการรักษา ประดู่ป่าได้รับการยอมรับว่าเป็นแหล่งของสารสำคัญในด้านการรักษาโรค สารสกัดจากแก่นของประดู่ป่ามีสารประกอบสำคัญคือ homopterocarpin ซึ่งมีคุณสมบัติต้านอนุมูลอิสระและต้านมะเร็งตับ อย่างไรก็ตาม ผลกระทบต่อมะเร็งลำไส้ใหญ่ยังไม่ชัดเจน ในการศึกษานี้ ผู้วิจัยศึกษาผลของสารสกัดหยาบจากเปลือกของประดู่ป่าต่อฤทธิ์ต้านอนุมูลอิสระและความมีชีวิตของเซลล์มะเร็งลำไส้ใหญ่ ตัวอย่างประดู่ป่าถูกเก็บและระบุชนิดในเดือนกุมภาพันธ์ พ.ศ.2568 จากนั้นแช่ตัวอย่างแห้งของเปลือกของประดู่ป่าใน methanol เป็นเวลา 48 ชั่วโมง ตัวอย่าง 20 กรัม ให้สารสกัด 3.79 กรัม การวิเคราะห์ TLC ปรากฏจุดสีแดงเด่นชัดในทุกครั้งของการทดสอบ คาดว่าเป็นสารประกอบหลักของสารสกัดคือ homopterocarpin ฤทธิ์ต้านอนุมูลอิสระของสารสกัดถูกวิเคราะห์ด้วยวิธี DPPH assay และคำนวณค่า IC50 เพื่อประเมินความสามารถในการกำจัดอนุมูลอิสระ ซึ่งอยู่ที่ 45 µg/ml การทดสอบความเป็นพิษของสารสกัดต่อเซลล์มะเร็งลำไส้ใหญ่คือ HCT116 และ SW620 และเซลล์ไตตัวอ่อนของมนุษย์คือ HEK293 ด้วยวิธี MTT assay คำนวณค่า IC50 เพื่อประเมินความสามารถในการกำจัดเซลล์มะเร็งและความเป็นพิษต่อไต ซึ่งมีค่า 91, 32 และ 87 µg/ml ตามลำดับ สารสกัดจากเปลือกของประดู่ป่าแสดงคุณสมบัติต้านอนุมูลอิสระและต้านมะเร็งลำไส้ใหญ่ที่โดดเด่น อย่างไรก็ตาม จำเป็นต้องมีการศึกษาเพิ่มเติมเพื่อศึกษากลไกการออกฤทธิ์ของสารประกอบแต่ละชนิดในสารสกัดนี้
Investigation And Computational Analysis Of The Iqd Gene Family In Common Bean (Phaseolus Vulgaris L.): Expression Profiling Under Salt Stress, Tarik Kirlioğlu, Aybüke Okay, Emi̇ne Sümer Aras, İlker Büyük
Turkish Journal of Botany
Common bean is one of the most nutritionally important legumes because of its high-quality protein content. While its genome has been widely studied however, the IQD gene family has not been extensively investigated in common bean to date. The IQD proteins (IQ-67 domain), a type of calmodulin-binding protein, are involved in plant growth and development, as well as the plant's response to environmental stressors. In this study, thirty-six PvIQD genes were discovered and their proteins were found to be classified into four groups according to the phylogenetic tree. The proteins which are encoded by PvIQD genes with similar exon-intron pattern …
Primary Distribution And Rt-Pcr-Based Detection Of Citrus Dwarfing Viroid (Cdvd) In Citrus Cultivars Of Pakistan,
2024
TÜBİTAK
Primary Distribution And Rt-Pcr-Based Detection Of Citrus Dwarfing Viroid (Cdvd) In Citrus Cultivars Of Pakistan, Zia Ul Haq, Amjad Ali, Syed A.H. Naqvi, Faheem Shehzad Baloch, Ummad U.D. Umar
Turkish Journal of Botany
Viroid diseases have emerged as a significant threat to citrus orchards globally. In Pakistan, viroid infections have rapidly spread across different citrus cultivar, posing serious challenges to the survival of orchards. This study encompasses the determination of the prevalence and detection of Citrus dwarfing viroid (CDVd) in both reported and newly introduced citrus cultivars in Pakistan using RT-PCR. RNA was extracted from 122 infected citrus samples and examined for the presence of viroids following the successful optimization of newly designed CDVd primers (CDVd AF1/AR1). This led to the confirmation of CDVd in 72 samples (59.01%).. Biological indexing was performed using …
Functional Characterization Of Haloarchaeal Udp-Glucose 4-Epimerase And 3-Hydroxybutyryl-Coa Dehydrogenase Genes In Nicotiana Tabacum, Çi̇ğdem Otur, Sezer Okay, Aslihan Kurt Kizildoğan
Turkish Journal of Botany
The objective of the studyAbiotic stress poses a significant threat to plant survival. In response to the challenging conditions, plants have evolved various resistance mechanisms. Recently, genetic strategies have become increasingly prevalent in efforts to enhance abiotic stress tolerance and develop stress-resistant plant varieties. In this study, we employed heterologous expression of two metabolism-related haloarchaeal genes in the Nicotiana tabacum genome to improve its mechanism for salt and drought tolerance.Experimental procedure(s)The U2642 and CL165 genes encoding 3-hydroxybutyryl-CoA dehydrogenase and UDP-glucose 4-epimerase, respectively, were subcloned into the pIPKb004 vector under the control of Cauliflower mosaic virus 35S (CaMV35S) promoter using the …
Genome-Wide Identification And Characterization Of The Lea Gene Family In Common Bean And The Role Of The Dehydrin Gene Pvhdn3 In Enhancing Salt And Drought Tolerance In Transgenic Tobacco Plant., Gökhan Gökdemi̇r, Zafer Seçgin, Karam Mostafa, Mohamed Farah Abdulla, Musa Kavas
Turkish Journal of Botany
Global climate change exposes crops to numerous abiotic and biotic stress factors, significantly threatening their growth, development, and overall productivity. Among the various abiotic stresses, salt and drought are the most detrimental, having a severe impact on yield stability. Late Embryogenesis Abundant proteins (LEA) represent a broad and varied class of polypeptides that accumulate in response to cellular dehydration across diverse organisms. Nonetheless, there has been a scarcity of studies on this protein family in common beans prior to this investigation. In this study, we identified 69 members of the LEA gene family in common bean, characterized by conserved LEA …
Phylogenomics Of The Tribe Leonureae Reveals A New Genus: Paraleonurus (Lamiaceae, Lamioideae),
2024
TÜBİTAK
Phylogenomics Of The Tribe Leonureae Reveals A New Genus: Paraleonurus (Lamiaceae, Lamioideae), Mei-Lin Qian, Ya-Ping Chen, Tuncay Di̇rmenci̇, Yue Zhao, Guo-Xiong Hu, Fei Zhao, Ferhat Celep, Bo Li, Chunlei Xiang
Turkish Journal of Botany
With approximately 80 species across six genera (Leonurus, Lagopsis, Panzerina, Lagochilus, Chaiturus, and Loxocalyx), the tribe Leonureae represents one of the most morphologically diverse tribes within the subfamily Lamioideae of Lamiaceae. Previous studies indicated that Leonurus and Lagopsis are not monophyletic, and relationships among and within these genera remain unclear due to limited sampling and DNA markers. In this study, we present the most comprehensive phylogenomic analyses of Leonureae based on complete plastome sequences and 127 nuclear loci retrieved from next-generation sequencing data. Our results provide strong evidence for the monophyly of Leonureae, which can be further divided into six …
Heterologous Expression Of Haloarchaeal Transporter Genes In Nicotiana Tabacum Under Salt And Drought Stresses,
2024
TÜBİTAK
Heterologous Expression Of Haloarchaeal Transporter Genes In Nicotiana Tabacum Under Salt And Drought Stresses, Çi̇ğdem Otur, Musa Kavas, Aslihan Kurt Kizildoğan
Turkish Journal of Botany
The objective of the study: Climate change and human activities impose severe biotic and abiotic stresses that threaten plant survival. To cope with these extreme conditions, plants have evolved various resistance mechanisms. In recent years, genetic approaches have been increasingly employed to enhance abiotic stress tolerance and develop stress-resistant plants. In this study, we introduced three transporter-related genes from extreme haloarchaea, which thrive in saturated NaCl environments, into the Nicotiana tabacum genome to improve its salt and drought tolerance mechanisms.E xperimental procedure(s) : The U2845, U3508, and CL165 genes were subcloned into the pIPKb004 vector, which includes the cauliflower mosaic …
Innovations In Plant Genome Editing And Biotechnology: Addressing Global Agricultural Challenges,
2024
TÜBİTAK
Innovations In Plant Genome Editing And Biotechnology: Addressing Global Agricultural Challenges, Bayram Ali̇ Yerli̇kaya, Karam Mostafa, Abdullah Aydin, Seher Yerli̇kaya, Momna Mehmood, Ni̇sa Nur Yilmaz, Kubi̇lay Yildirim, Musa Kavas
Turkish Journal of Botany
Developments in genome editing and plant biotechnology in recent years, especially CRISPR-Cas9 and RNA interference (RNAi) technologies, offer essential tools for solving global agricultural problems that are worsened by climate change and the increasing food demand caused by the rising world population. This review focuses on the urgent need to feed a growing global population. It explores how key biotechnological tools can enhance crop resilience and nutritional quality amid climate variability and extreme weather. The applicability of CRISPR-Cas technologies in the development of climate-friendly, more nutritious, and higher-yielding crops has opened a biotechnological avenue for the development of both transgenic …
Advancements In Qtl Mapping And Gwas Application In Plant Improvement,
2024
TÜBİTAK
Advancements In Qtl Mapping And Gwas Application In Plant Improvement, Muhammad Tanveer Altaf, Muhammed Tatar, Amjad Ali, Waqas Liaqat, Parnaz Mortazvi, Ceyhun Kayihan, Fati̇h Ölmez, Muhammad Azhar Nadeem, Jazib Javed, Jin-Ying Gou, Meng-Lu Wang, Ummad Ud Din Umar, Hayri̇ye Yildiz Daşgan, Cemal Kurt, Mehtap Yildiz, Sheikh Mansoor, Abdelfattah A. Dababat, Nafiz Çeli̇ktaş, Faheem Shehzad Baloch
Turkish Journal of Botany
Molecular markers play a crucial role in modern plant breeding by enabling the precise identification of genetic loci associated with important agronomic traits. This article focuses on the advancements in Quantitative Trait Locus (QTL) mapping and Genome-Wide Association Studies (GWAS), which are pivotal in identifying and dissecting complex traits such as stress tolerance, yield improvement, and nutrient efficiency. Through QTL mapping, significant genetic regions linked to desired traits are identified, while GWAS leverages larger populations for higher resolution in genetic association studies. The integration of next-generation sequencing, CRISPR, and transcriptomics technologies has revolutionized the precision and efficiency of these methods, …
Identification Of Genes Associated With Zinc And Copper Concentrations In Walnut (Juglans Regia L.) By Gwas Analysis,
2024
TÜBİTAK
Identification Of Genes Associated With Zinc And Copper Concentrations In Walnut (Juglans Regia L.) By Gwas Analysis, Berfi̇n Nazlican Kaman, Bülent Yağmur, Erdal Orman, Muhammed Bahatti̇n Tanyolaç, Duygu Ateş
Turkish Journal of Botany
154 walnut genotypes from various geographic regions were analyzed in the study. Micronutrient concentrations of zinc (Zn) ranged from 1.61 to 8.98 mg/100 g, and the concentrations of copper (Cu) varied between 0.12 and 0.38 mg/100 g. 16,473 SNPs were generated using Diversity Array Technology (DArT) for genome-wide association (GWAS) studies. The Mixed Linear Model (MLM) utilizing the Q + K model revealed seven associated markers for the element Zn in 2021 and four in 2022. The MLM (Q + K) model indicated two associated markers for the element Cu in 2021 and three in 2022. The markers found in …
Gene Expression Changes In The Seagrass Cymodocea Nodosa Individuals In Response To Aquatic Acidification,
2024
Ege University
Gene Expression Changes In The Seagrass Cymodocea Nodosa Individuals In Response To Aquatic Acidification, Enes Göksal, Esra Öztürk Yi̇ği̇t
Turkish Journal of Botany
Human activities have caused a rise in atmospheric carbon dioxide (CO2) levels, leading to greater absorption of CO2 by oceans and causing ocean acidification. This phenomenon, marked by a reduction in pH, represents substantial risks to marine ecosystems, including seagrass meadows. Seagrasses are vital elements of coastal ecosystems, performing important functions in carbon storage, stabilizing shorelines, and preserving biodiversity; however, reactions to ocean acidification are not well understood, especially in terms of molecular.This research study examined alterations in gene expression within seagrass meadows, namely the species Cymodocea nodosa, in reaction to simulated ocean acidification conditions. Climate chamber system to adjust …
Exploring The Population Structure And Genetic Diversity In Apple Germplasm Using Ipbs-Retrotransposon Markers,
2024
TÜBİTAK
Exploring The Population Structure And Genetic Diversity In Apple Germplasm Using Ipbs-Retrotransposon Markers, Aigul Madenova, Angsagan Kuan, Amjad Ali, Muhammad Azhar Nadeem, Muhammad Tanveer Altaf, Balnur Kabylbekova, Dinara Kaldybaeva, Ti̇mur Turdi̇yev, Faheem Shahzad Baloch
Turkish Journal of Botany
The apple (Malus domestica [Suckow] Borkh) is economically and culturally significant worldwide, thriving in temperate regions. This study examined genetic variations among 52 apple accessions from three research centers in Kazakhstan using the iPBS-retrotransposons marker system. Out of 35 screened markers, 12 highly polymorphic markers were selected for PCR amplification, resulting in 280 bands, 279 of which were polymorphic, yielding a 99.64% polymorphism rate. Genetic diversity indices showed substantial variation: effective number of alleles (ne=1.655), Shannon's information index (I=0.373), gene diversity (h=0.549), and an average genetic distance of 1.2. The analysis of molecular variance (AMOVA) indicated that 98.80% of the …
Role Of Sulfur Metabolism In Acquiring Of Boron Tolerance In Arabidopsis Thaliana,
2024
TÜBİTAK
Role Of Sulfur Metabolism In Acquiring Of Boron Tolerance In Arabidopsis Thaliana, Seda Yi̇rmi̇beş, Ceyhun Kayihan, Nuran Çi̇çek, Yasemi̇n Ekmekçi̇
Turkish Journal of Botany
The purpose of this study was to assess whether sulfate applications (pre- and combined) caused the changes of tolerance against boron (B) toxicity in Arabidopsis thaliana at physiological, biochemical and molecular levels. Plants were germinated and grown on MS medium for 12 days, and then, sulfate pre-treatment groups (PS-B) were transferred to sulfate-containing media for 3 days. Subsequently, PS-B, combined (S+B) and un-treated application groups were transferred to medium containing 3 and 5 mM B for 10 days. Leaf area, plant biomass and total chlorophyll content decreased significantly depending on the accumulation of B. Toxic levels of B adversely affected …
Selective Indel Marker Identification Across The Peanut (Arachis Hypogaea L.) Genome Using Ddradseq,
2024
TÜBİTAK
Selective Indel Marker Identification Across The Peanut (Arachis Hypogaea L.) Genome Using Ddradseq, Moin Qureshi, Bi̇rgül Guden, Hi̇lal Şule Tosun, Mojtaba Kordrostami, Sunil Shiwaji Gangurde, Bülent Uzun, Engi̇n Yol
Turkish Journal of Botany
The advent of next-generation sequencing technologies, mainly double digest restriction site-associated DNA sequencing (ddRADSeq), has significantly advanced the development of molecular markers for crop genetics. This study used ddRADSeq to identify and develop insertion-deletion (InDel) markers in 25 peanut genotypes from diverse geographic regions. The bioinformatic analysis unveiled 62728 InDels across the peanut genome, predominantly between 1-5 bp, which constituted 96% of the total, while InDels of ≥ 6 bp accounted for 3.96%. We focused on 1013 InDels of at least 10 bp for further analysis, representing 1.61% of the total reads, with a distribution of 832 insertions and 181 …
Roquea, A New Genus Of Lychnophorinae (Vernonieae, Asteraceae) From Brazil And Its Phylogenetic Placement,
2024
Royal Botanic Gardens, Kew
Roquea, A New Genus Of Lychnophorinae (Vernonieae, Asteraceae) From Brazil And Its Phylogenetic Placement, Benoit Loeuille, Roberto Baptista Pereira Almeida, Carolina Moriani Siniscalchi, Makeli G. Lusa, Guilherme Medeiros Antar
UL Publications
Lychnophorinae (Vernonieae, Asteraceae) consists of 21 genera and approximately 150 species, primarily distributed in the Brazilian Central Plateau, with several novelties recently described. With new botanical expeditions conducted in Serra da Formosa, state of Minas Gerais, a set of collections that belong to Lychnophorinae but were not easily assigned to a genus was discovered. After morphological and anatomical analyses and phylogenetic studies, we describe and illustrate Roquea, a new genus of Asteraceae from Brazil, to accommodate the new species Roquea multiserialis. Affinities of the new genus with other genera of Lychnophorinae are discussed along with a preliminary conservation …
Leveraging Within-Field Spatial Variability To Optimize Soybean Yield, Seed Quality, And Profitability,
2024
University of Nebraska-Lincoln
Leveraging Within-Field Spatial Variability To Optimize Soybean Yield, Seed Quality, And Profitability, Marina Marly Dalla Betta
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Optimizing yield and seed quality in soybean production is critical for both profitability and meeting market demands. However, within-field variability in soil properties poses a challenge in determining optimal management strategies to maximize soybean yield, seed quality, and profitability. This work aimed to evaluate the spatial variability of soybean fields to optimize soybean seeding rates, seed quality, and profitability. Two case studies were used to carry over this research. The first study looked at the optimal economical site-specific soybean seeding rates using 11 on-farm precision experiments across Nebraska. The second study looked at the protein and oil variability within 22 …
Evaluating The Roles Of Pollinators In Shaping The Floral Color Variation Of Phacelia Dubia,
2024
Clemson University
Evaluating The Roles Of Pollinators In Shaping The Floral Color Variation Of Phacelia Dubia, Katherine Riddle
All Theses
Floral color variation within a species is a common occurrence and its maintenance has been a long-standing question in evolutionary biology. Differences in flower color between populations can be shaped by pollinator-mediated selection. Pollinators can exhibit innate and learned preferences for color that may influence floral color variation. These preferences may be molded by the visual environment in which pollinators forage. Most current understanding of how pollinators shape floral color variation involves dramatic differences in dominant pollinators between populations (e.g., hawkmoth vs. bee) and most behavioral work utilized model pollinators like honeybee and bumble bee. However, less is known about …
