Investigating Distribution Of Legionella Pneumophila In Urban And Suburban Watersheds,
2021
CUNY Queens College
Investigating Distribution Of Legionella Pneumophila In Urban And Suburban Watersheds, Azlan Maqbool
Student Theses
The presence of Legionella pneumophila was assessed using a cultivation-based approach in New York City waterways, a freshwater portion of the lower Hudson River Estuary near Kingston NY, and in urban and suburban street water. Legionella pneumophila was detected in 51% of brackish New York City Estuary samples, most with concentrations near minimum detection (=>1 organism/ mL). In contrast, it was detected in 22% of suburban freshwater Hudson River Estuary samples. Levels of the bacterium were found to be higher during wet weather compared to dry weather in the highly dense urban setting, but not in the less dense …
Detecting Bacterial Species From Ancient Human Skeletal Samples,
2021
Kennesaw State University
Detecting Bacterial Species From Ancient Human Skeletal Samples, Ariel Owens, Daisy Mcgrath, Tsai-Tien Tseng
Symposium of Student Scholars
This paleopathological study aims to identify Mycobacterium tuberculosis complex (MTBC), Mycobacterium avium complex (MAC) and other Mycobacterium species in silico from skeletal samples that belonged to 28 Polish individuals in the Neolithic period under PRJNA422903 from the Sequence Read Archive (SRA). After next-generation sequencing (NGS), bioinformatics methods are heavily relied upon for identification of pathogens from complex samples. We implemented a bioinformatics pipeline, with custom-built databases, utilizing the following software tools: Trim Galore! and Kraken2. After adapter trimming, Kraken2 was used for taxonomic classifications. We have found that Mycobacterium is present in all 28 individuals. The average percentage of MAC …
Profile Of Antimicrobial Susceptibility From Cattles’S Milk Isolates Suffering From Mastitis In District Lahore,
2021
Department of Zoology, University of the Punjab, Lahore, Pakistan
Profile Of Antimicrobial Susceptibility From Cattles’S Milk Isolates Suffering From Mastitis In District Lahore, Muhammad Imran, Iqra Rehman, Abdul Qayyum Khan Sulehria, Yasser Mustafa Butt, Abdul Majid Khan, Azizah Ziauddin
Journal of Bioresource Management
Mastitis is highly prevalent infection in cattle causing cost-effective loss in dairy milk production. Escherichia coli is the most frequently isolated bacteria causing mastitis worldwide. The current study was performed to investigate the mastitis prevalence and effect of different antibiotics against pathogens causing it. In sum, 216 milk samples were collected randomly including 108 each from both cows and buffaloes. These samples were subjected to Surf Field Mastitis Test for prevalence of mastitis that found 18.50% and 23.14% in buffaloes and cows respectively. Mastitis was more prevalent both in buffaloes and cows at the age of 9-10 years that was …
Sickness Behaviors Across Vertebrate Taxa: Proximate And Ultimate Mechanisms,
2021
Chapman University
Sickness Behaviors Across Vertebrate Taxa: Proximate And Ultimate Mechanisms, Patricia C. Lopes, Susannah S. French, Douglas C. Woodhams, Sandra A. Binning
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
There is nothing like a pandemic to get the world thinking about how infectious diseases affect individual behavior. In this respect, sick animals can behave in ways that are dramatically different from healthy animals: altered social interactions and changes to patterns of eating and drinking are all hallmarks of sickness. As a result, behavioral changes associated with inflammatory responses (i.e. sickness behaviors) have important implications for disease spread by affecting contacts with others and with common resources, including water and/or sleeping sites. In this Review, we summarize the behavioral modifications, including changes to thermoregulatory behaviors, known to occur in vertebrates …
Diagnostic Approaches To Combat Antibiotic Resistance In Bacteria,
2021
University of Mississippi
Diagnostic Approaches To Combat Antibiotic Resistance In Bacteria, Alexander Oliveri, Shade Smith, Anshul Anugu
Honors Theses
The threat of antibiotic resistance is a major problem faced by the healthcare field affecting millions of people and costing tens of thousands of lives annually. Of the potential ways to mitigate this issue the field of antibiotic resistance testing presents an opportunity for significant improvement and benefits. Several methods of such diagnostic processes can yield more informative results than the current commonly used Kirby-Bauer test. However, there are benefits and limitations to each method. In the context of a clinically relevant diagnostic for antibiotic resistance, the microarray platform exhibits the necessary breadth with opportunities to overcome some limitations with …
Vignette 14: Eelgrass Wasting Disease,
2021
Cornell University
Vignette 14: Eelgrass Wasting Disease, Olivia Graham, Morgan Eisenlord, Drew Harvell
Institute Publications
Rising seawater temperatures can increase the risk of disease outbreaks in many taxa. Pathogens are potentially the ultimate keystone species in that their small biomass can have massive impacts that ripple through ecosystems. Disease outbreaks can be particularly damaging when they affect ecosystem engineers, such as seagrasses. Outbreaks of wasting disease in seagrasses are one of a myriad of stressors associated with declining temperate and tropical seagrass meadows around the globe. Levels of eelgrass wasting disease are high in the San Juan Islands and Puget Sound. These increasing levels of disease are a threat to sustainability of eelgrass meadows, our …
Antibiotic Resistance, A Global Pandemic,
2021
University of Tennessee, Knoxville
Antibiotic Resistance, A Global Pandemic, Deneil Patel
Chancellor’s Honors Program Projects
No abstract provided.
Pathogenicity Of Staphylococcus Agnetis Associated With Lame Broilers With Osteomyelitis,
2021
University of Arkansas, Fayetteville
Pathogenicity Of Staphylococcus Agnetis Associated With Lame Broilers With Osteomyelitis, Sura Faris Salih Zaki
Graduate Theses and Dissertations
We developed High Resolution Melt Analysis (HERMA) as a rapid and reliable molecular diagnostic assay for the detection and identification of the main bacterial species recovered from the blood and lesions of bacterial chondronecrosis with osteomyelitis (BCO) from the lame broilers at the University of Arkansas poultry research farm. Also, the present study confirms that raising young birds on suspended wire flooring has been proved to successfully induce lameness attributable to BCO with birds being more susceptible to bacteraemia than those that were raised on litter flooring. The newly described pathogen, Staphylococcus agnetis, has been reported to be overrepresented in …
The Impact Of Oxygen Availability And Oxidative Stress On Regulation Of Cyclic-Dimeric-Gmp In Listeria Monocytogenes Strain F2365,
2021
The University of Southern Mississippi
The Impact Of Oxygen Availability And Oxidative Stress On Regulation Of Cyclic-Dimeric-Gmp In Listeria Monocytogenes Strain F2365, Anna G. Welch
Honors Theses
Listeria monocytogenes is a gram-positive foodborne pathogen that results in the infectious disease listeriosis. The second messenger molecule cyclic-dimeric-GMP has been found to be responsible for the regulation of expression of many of its virulence factors with diguanylate cyclases and phosphodiesterases regulating the intracellular concentration of cyclic-di-GMP through synthesis and degradation, respectively. This study investigated the possibility that the availability of oxygen is the environmental signal crucial to the regulation of these enzymes. Prior research in our laboratory has demonstrated that the intracellular concentration of cyclic-di-GMP is elevated when exposed to anaerobic conditions. Due to this, it was hypothesized that …
Study Of The Role Of The Msaabcr Operon In Tricarboxylic Acid Cycle Activity And Persister Cell Formation In Staphylococcus Aureus,
2021
The University of Southern Mississippi
Study Of The Role Of The Msaabcr Operon In Tricarboxylic Acid Cycle Activity And Persister Cell Formation In Staphylococcus Aureus, Karsen Motter
Honors Theses
Staphylococcus aureus is an important human pathogen that causes wide arrays of infections ranging from minor skin infections to lethal systemic conditions such as infective endocarditis, osteomyelitis, sepsis and pneumonia. These systemic diseases are often difficult to treat due to the presence of persister cells. Persister cells are a phenotypic variant of the bacterial population that exhibit extreme and transient antibiotic tolerance accompanied by a transient halt in growth. Upon cessation of antibiotic treatment, however, persisters resume growth which results in recurrence of infections. This characteristic of persister cells therefore displays high clinical significance. In this study, we show the …
Interregulation Between Msaabcr Operon And Ccpe To Determine Staphylococcal Metabolism And Virulence,
2021
The University of Southern Mississippi
Interregulation Between Msaabcr Operon And Ccpe To Determine Staphylococcal Metabolism And Virulence, Erin R. Cox
Honors Theses
Staphylococcus aureus is a complex human pathogen that causes problems in both healthcare and community settings. Staphylococcus aureus is a gram-positive, sphere shaped bacterium that usually colonizes in the nasal cavity of healthy individuals. Staphylococcus aureus infections are a growing health concern due to its ability to produce virulence factors, such as pigmentation, protease production, and capsule formation. Two regulators in S. aureus virulence factors are msaABCR, which is a newly characterized operon, and ccpE. In order to determine the interaction between these two regulators in regulating virulence and metabolism in S. aureus, ccpE and ccpE/ …
Phenotypic Characterizations Of Msaabcr Operon Deletion In Staphylococcus Epidermidis Rp62a,
2021
The University of Southern Mississippi
Phenotypic Characterizations Of Msaabcr Operon Deletion In Staphylococcus Epidermidis Rp62a, Raelyn Williams
Honors Theses
Staphylococcus epidermidis is a human pathogen that is increasingly known for its role in hospital infections associated with implantable medical devices. Antibiotic resistance has become a concerning issue for these infections as this bacteria have various virulence traits that help to evade immune response and antibiotic treatment. Currently, the most effective way to treat S. epidermidis infection is removal of the implant and long-term antibiotic treatment. S. epidermidis causes infection by expressing several protein factors that induce biofilm formation, the bacteria’s primary virulence mechanism. The purpose of this study was to perform the phenotypic characterizations of the msaABCR operon in …
Sodium Pyruvate Ameliorates Influenza A Virus Infection In Vitro And In Vivo,
2021
Missouri State University
Sodium Pyruvate Ameliorates Influenza A Virus Infection In Vitro And In Vivo, Jessica M. Reel
MSU Graduate Theses
Pyruvate is produced in duplicate at the end of glycolysis in addition to ATP and NADH. Pyruvate is the metabolite of choice in most cells, whether obtained exogenously or endogenously. Recently we found that the addition of pyruvate’s conjugate base, sodium pyruvate, to cell culture media dampened the immune response to influenza A virus (IAV) infection in cultured innate immune cells. Thus, I decided to investigate the mechanism and potential for treatment of IAV. In vitro using bone marrow derived macrophages that were infected with IAV we found that adding sodium pyruvate to the media decreased immune signaling pathways through …
A Metabolomics-Based Approach To The Screening Of Endometrial Cancer: Development Of A Gas Chromatography-Ion Trap Mass Spectrometry-Based Method,
2021
University of Tennessee at Chattanooga
A Metabolomics-Based Approach To The Screening Of Endometrial Cancer: Development Of A Gas Chromatography-Ion Trap Mass Spectrometry-Based Method, Allycia Lee
Honors Theses
Endometrial Cancer (EC) is the most common malignant tumor of the female reproductive tract. Currently there are no methods for effectively screening EC, but one method that is thought to be useful in screening EC is metabolomics. Metabolomics involves the analysis of metabolites which are low molecular weight inorganic and organic chemicals that are substrates, intermediates, and by-products of enzyme-mediated biochemical reactions in the cell. Previous research shows that the metabolic signature of EC patients are discernable from those of healthy patients. To aid in the development of an effective screening method for EC, blood serum, urine, and saliva samples …
Process Interventions For Improving The Microbiological Safety Of Low Moisture Food Ingredients,
2021
University of Nebraska - Lincoln
Process Interventions For Improving The Microbiological Safety Of Low Moisture Food Ingredients, Tushar Verma
Dissertations, Theses, & Student Research in Food Science and Technology
The recurrence of Salmonella in low moisture foods and the implementation of the FSMA rule requires a need to validate legacy and novel processing technologies. In this dissertation, a legacy thermal (extrusion), a novel thermal (radiofrequency (RF) heating), and a non-thermal (chlorine dioxide) technology, were evaluated as intervention technologies for Salmonella in low moisture foods. The twin-screw extruder was performed at different levels of screw speeds, temperatures, moisture contents, and fat contents to understand the impact of processing conditions on Salmonella inactivation in oat flour. At temperature >65°C, the Salmonella population was below the detection limit. At 55°C, Salmonella reduction …
Respiratory Pathogens In Patients With Acute Exacerbation Of Non-Cystic Fibrosis Bronchiectasis From A Developing Country,
2021
Aga Khan University
Respiratory Pathogens In Patients With Acute Exacerbation Of Non-Cystic Fibrosis Bronchiectasis From A Developing Country, Shayan Shahid, Kausar Jabeen, Nousheen Iqbal, Joveria Farooqi, Muhammad Irfan
Medical College Documents
Bronchiectasis unrelated to cystic fibrosis (non-CF bronchiectasis) has become a major respiratory disease in developing nations. The dilated mucus filled airways promote bacterial overgrowth followed by chronic infection, bronchial inflammation, lung injury and re-infection Accurate pathogen identification and antimicrobial susceptibility allowing appropriate treatment, in turn, may break this vicious cycle. To study the spectrum and antimicrobial spectrum of pathogen yielded from respiratory specimens in adult patients with acute exacerbation of non-cystic fibrosis (CF) bronchiectasis. This cross-sectional study was performed at the pulmonology clinics of the Aga Khan University, Karachi, Pakistan from 2016-2019. Respiratory specimens were collected from adult patients with …
Molecular Surveillance Of Drug Resistance: Plasmodium Falciparum Artemisinin Resistance Single Nucleotide Polymorphisms In Kelch Protein Propeller (K13) Domain From Southern Pakistan,
2021
Aga Khan University
Molecular Surveillance Of Drug Resistance: Plasmodium Falciparum Artemisinin Resistance Single Nucleotide Polymorphisms In Kelch Protein Propeller (K13) Domain From Southern Pakistan, Najia Karim Ghanchi, Bushra Qurashi, Hadiqa Raees, Mohammad Asim Beg
Department of Pathology and Laboratory Medicine
Background: K13 propeller (k13) polymorphism are useful molecular markers for tracking the emergence and spread of artemisinin resistance in Plasmodium falciparum. Polymorphisms are reported from Cambodia with rapid invasion of the population and almost near fixation in south East Asia. The study describes single nucleotide polymorphisms in Kelch protein propeller domain of P. falciparum associated with artemisinin resistance from Southern Pakistan.
Methods: Two hundred and forty-nine samples were collected from patients with microscopy confirmed P. falciparum malaria attending Aga Khan University Hospital during September 2015-April 2018. DNA was isolated using the whole blood protocol for the QIAmp DNA Blood Kit. …
Effectiveness Of Pathogen-Specific Passive Antibodies To Mitigate Infectious Diseases In Apis Mellifera,
2021
University of South Dakota
Effectiveness Of Pathogen-Specific Passive Antibodies To Mitigate Infectious Diseases In Apis Mellifera, Tanner Nordseth
Honors Thesis
Honeybees (Apis mellifera) are widely recognized as a vital part of the global ecosystem and the world's food supply due to their pivotal role in the pollination of both natural and agricultural flora. Colony Collapse Disorder (CCD) is an emerging phenomenon characterized by a colony's worker bees deserting the hive and leaving the queen behind. This usually results in colony failure. CCD is a multifactorial issue, with many environmental stressors and pathogens playing a role. Deformed Wing Virus (DWV) has been identified as a leading cause in this phenomenon. Paenibacillus larvae (P. larvae) is another lethal pathogen that is …
Cloning Of Pe11 (Lipx, Rv1169c) Gene Of Mycobacterium Tuberculosis Beijing Strain To Pcdna3.1 Plasmid Vector,
2021
Biomedical Student, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
Cloning Of Pe11 (Lipx, Rv1169c) Gene Of Mycobacterium Tuberculosis Beijing Strain To Pcdna3.1 Plasmid Vector, Lulut Azmi Supardi, Andriansjah Rukmana, Fithriyah Sjatha
Makara Journal of Science
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. It is a persistent global health problem with a high mortality rate. Currently, TB is controlled by administering the Bacillus Calmette-Guerin (BCG) vaccine, but the effectiveness of its protection varies among individuals in a population. The pe/ppe gene family comprises a typical group of genes that play a role in avoiding the host immune response and inducing persistent TB infection. Based on in silico analysis, the pe11 gene has estimated immunogenicity and potential as a TB seed vaccine candidate. The pe11 gene from an Indonesian isolate of an M. …
Pirnas As Modulators Of Disease Pathogenesis,
2021
Meharry Medical College
Pirnas As Modulators Of Disease Pathogenesis, Kayla J. Rayford, Ayorinde Cooley, Jelonia T. Rumph, Ashutosh Arun, Girish Rachakonda, Fernando Villalta, Maria F. Lima, Siddharth Pratap, Smita Misra, Pius N. Nde
Publications and Research
Advances in understanding disease pathogenesis correlates to modifications in gene expression within different tissues and organ systems. In depth knowledge about the dysregulation of gene expression profiles is fundamental to fully uncover mechanisms in disease development and changes in host homeostasis. The body of knowledge surrounding mammalian regulatory elements, specifically regulators of chromatin structure, transcriptional and translational activation, has considerably surged within the past decade. A set of key regulators whose function still needs to be fully elucidated are small non-coding RNAs (sncRNAs). Due to their broad range of unfolding functions in the regulation of gene expression during transcription and …
