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Investigating Distribution Of Legionella Pneumophila In Urban And Suburban Watersheds, Azlan Maqbool 2021 CUNY Queens College

Investigating Distribution Of Legionella Pneumophila In Urban And Suburban Watersheds, Azlan Maqbool

Student Theses

The presence of Legionella pneumophila was assessed using a cultivation-based approach in New York City waterways, a freshwater portion of the lower Hudson River Estuary near Kingston NY, and in urban and suburban street water. Legionella pneumophila was detected in 51% of brackish New York City Estuary samples, most with concentrations near minimum detection (=>1 organism/ mL). In contrast, it was detected in 22% of suburban freshwater Hudson River Estuary samples. Levels of the bacterium were found to be higher during wet weather compared to dry weather in the highly dense urban setting, but not in the less dense suburban ...


Evidence For Perchlorate-Coupled Molybdenum And Nickel Carbon Monoxide Dehydrogenase Co Oxidation And Characterization Of Novel Perchlorate-Reducing Haloarchaea, Marisa Russell Myers 2021 Louisiana State University and Agricultural and Mechanical College

Evidence For Perchlorate-Coupled Molybdenum And Nickel Carbon Monoxide Dehydrogenase Co Oxidation And Characterization Of Novel Perchlorate-Reducing Haloarchaea, Marisa Russell Myers

LSU Doctoral Dissertations

Carbon monoxide (CO) has been exploited as a microbial energy source for much of life’s evolutionary history. A phylogenetically diverse array of microorganisms can oxidize CO using two distinct CO dehydrogenases, molybdenum-dependent (Mo-CODH) and nickel-dependent (Ni-CODH). Aerobes and facultative organisms contain Mo-CODHs which allow them to utilize oxygen as an electron acceptor in addition to alternatives such as nitrate and sulfate. Obligate anaerobic organisms contain Ni-CODHs, which oxidize CO at elevated concentrations, but cannot utilize oxygen. In systems where organic matter deposits are limited or absent, atmospheric trace gases such as CO are thought to assist in supporting the ...


Identification And Comparison Of Arbuscular Mycorrhizal Fungi And Soil Microbiomes Between American Chestnuts And Surrounding Hardwoods, Sarah Andrews, Geoffrey Eger, Isabella Vahle 2021 Kennesaw State University

Identification And Comparison Of Arbuscular Mycorrhizal Fungi And Soil Microbiomes Between American Chestnuts And Surrounding Hardwoods, Sarah Andrews, Geoffrey Eger, Isabella Vahle

Symposium of Student Scholars

Background/Questions/Methods

The introduction of the Chinese chestnut blight in 1904 decimated native American chestnut (Castanea dentata (Marshall) Borkh.) populations. In this study we aim to: 1) document the location of individual chestnuts in our vicinity; 2) document healthy versus blight infected individuals; 3) characterize putative differences in the soil microbiome between infected and non-infected individuals, as well as between chestnuts and hardwood neighbors. With this information we hope to provide new insights into mechanisms that may enhance blight and fungal resistance in American chestnuts through the understanding of the interactions between the trees and their surrounding soil microbiome ...


Fungi Associated With Herbaceous Plants In Coastal Northern California, Greg Huffman 2021 Dominican University of California

Fungi Associated With Herbaceous Plants In Coastal Northern California, Greg Huffman

Natural Sciences and Mathematics | Biological Sciences Master's Theses

The presence of fungal species associated with herbaceous plants was monitored in coastal Marin County, California, USA. The research involved a combination of field sampling surveys and data collection using a stratified random design, pathogen identification through microbiological and molecular analysis, and descriptive analysis and ordination of results. A total of two years of repeated sampling (four times a year) was organized to allow for the detection of seasonal differences in pathogen presence on aerial plant parts. The objective of this study was to identify microbial species present on herbaceous plants using ITS1 sequence analysis. Throughout March of 2018 to ...


Project Alien, Rebecca McCallin, Madelyn Hoying, Alex Evans, Matthew Nestler, Karli Rae Sutton, Garett Craig, Lucia Secaia Del Cid, Alexander Guy, Rachel Fernandez, Amanda Trusiak, Paige Aley, Ingabire Gakwerere, Nina Dorfner, Maria Mosbacher, Mary Flavin, Selvin Hernandez, Audrey Steen, Benjamin Kazimer 2021 Duquesne University

Project Alien, Rebecca Mccallin, Madelyn Hoying, Alex Evans, Matthew Nestler, Karli Rae Sutton, Garett Craig, Lucia Secaia Del Cid, Alexander Guy, Rachel Fernandez, Amanda Trusiak, Paige Aley, Ingabire Gakwerere, Nina Dorfner, Maria Mosbacher, Mary Flavin, Selvin Hernandez, Audrey Steen, Benjamin Kazimer

Undergraduate Research and Scholarship Symposium

Project ALIEN is a comprehensive plan to send humans to Mars to look for life on the Martian surface while exploring the viability and adaptability of terrestrial microbes in Martian atmospheric conditions. ALIEN will use a ballistic capture trajectory to get to Mars and stay in aerostationary orbit for a 30-day surface mission, during which two surface crewmembers will perform a variety of experiments to achieve the mission’s goals of Martian microbial discovery within brines of the Gale Crater and terrestrial microbe adaptability and viability to Martian conditions. Experimentation is based on the presumption that Martian microbes are metabolically ...


Resistance Of The Freshwater Microbiome To A Combination Of Pesticides, Gabriel Hooper 2021 Liberty University

Resistance Of The Freshwater Microbiome To A Combination Of Pesticides, Gabriel Hooper

Senior Honors Theses

Pesticides are used prevalently throughout the United States and can negatively affect non-target organisms. Rain run-off can move multiple pesticides into freshwater ecosystems and bring them in contact with non-target organisms that have variable tolerances to pollution. This study seeks to improve our understanding of how an acute exposure to a combination of pesticides may restructure microbial communities by examining the microbiome before and after a 48-hour exposure to 2000 ppb of herbicides (glyphosate, atrazine, and 2,4-D). Pesticide exposure did not significantly alter the alpha or beta diversity of the sample types, which demonstrates the ability of freshwater microbiomes ...


Microbially-Mediated Carbonate Dissolution And Precipitation; Towards A Protocol For Ex-Situ, Cave-Analogue Cultivation Experiments, Vanessa E. Johnston, Andrea Martín-Pérez, Sara Skok, Janez Mulec 2021 Research Centre of the Slovenian Academy of Sciences and Arts, Slovenia

Microbially-Mediated Carbonate Dissolution And Precipitation; Towards A Protocol For Ex-Situ, Cave-Analogue Cultivation Experiments, Vanessa E. Johnston, Andrea Martín-Pérez, Sara Skok, Janez Mulec

International Journal of Speleology

Subterranean calcite dissolution and precipitation are often considered as strictly geochemical processes. The active involvement of microbes in these processes is commonly underestimated in the literature due to general oligotrophic conditions in caves, except in particular cave conditions, such as sulfidic caves and moonmilk deposits, where the presence of microbes likely plays a key role in mineral deposition. Here, we study the possible involvement of microbes from Postojna Cave, Slovenia, in carbonate dissolution (litholysis) and precipitation (lithogenesis). Microbes were sampled from small pools below hydrologically diverse drip sites and incubated on polished limestone tablets at 10 and 20°C for ...


Microplastics: Nacogdoches County’S Hidden Threat, Jordan Stanford, Sydney Matlock, Courtney Sims, Samantha Montes 2021 Stephen F Austin State University

Microplastics: Nacogdoches County’S Hidden Threat, Jordan Stanford, Sydney Matlock, Courtney Sims, Samantha Montes

Undergraduate Research Conference

Microplastics and their threat to marine environments are well known, but studies in freshwater environments are few and far between. Microplastics can be created by the breaking down of plastics or in the process of making plastics. They affect biological lifeforms in a multitude of ways, water temperature, permeability of the sediment. Pollution in Texas waterways have motivated groups like the Environmental Protection Agency, Trash Free Texas, and Environment Texas to get involved. The objectives of this study are two analyze Bonita and La Nana Bayou for microplastic presence and to educate and create awareness for Nacogdoches County on microplastics ...


Seasonal Variation Of E. Coli Abundance And Antibiotic Resistance Patterns In Water And Sediment Samples From Two Creeks With Different Potential Fecal Contamination Sources., James S. Zumhagen, Timothy Gsell 2021 Governors State University

Seasonal Variation Of E. Coli Abundance And Antibiotic Resistance Patterns In Water And Sediment Samples From Two Creeks With Different Potential Fecal Contamination Sources., James S. Zumhagen, Timothy Gsell

GSU Research Day

Escherichia coli has served as the primary indicator for fecal contamination for the past 30 years, but more recent studies suggest that this bacteria may survive the cold weather in the soil making it difficult to tell whether it is new contamination or old contamination. Studies have found that exposure to various contaminants such as heavy metals, water treatment chemicals, agricultural runoff, fecal contamination, and antibiotics, creates an increase in the resistance of the microbes exposed to it. Wastewater treatment facilities, animal hospitals and wastewater sites increase the bacterial load and the prevalence of antibiotic resistance of bacteria residing bacteria ...


Niche Differentiation Of Bacteria And Fungi In Carbon And Nitrogen Cycling Of Different Habitats In A Temperate Coniferous Forest: A Metaproteomic Approach, Robert Starke, Rubén López Mondéjar, Zander Rainer Human, Diana Navrátilová, Martina Štursová, Tomáš Větrovský, Heather M. Olson, Daniel J. Orton, Stephen J. Callister, Mary S. Lipton, Adina Howe, Lee Ann McCue, Christa Pennacchio, Igor Grigoriev, Petr Baldrian 2021 Institute of Microbiology of the Czech Academy of Sciences

Niche Differentiation Of Bacteria And Fungi In Carbon And Nitrogen Cycling Of Different Habitats In A Temperate Coniferous Forest: A Metaproteomic Approach, Robert Starke, Rubén López Mondéjar, Zander Rainer Human, Diana Navrátilová, Martina Štursová, Tomáš Větrovský, Heather M. Olson, Daniel J. Orton, Stephen J. Callister, Mary S. Lipton, Adina Howe, Lee Ann Mccue, Christa Pennacchio, Igor Grigoriev, Petr Baldrian

Agricultural and Biosystems Engineering Publications

Temperate coniferous forests sustain the highest levels of biomass of all terrestrial ecosystems and belong to the major carbon sinks on Earth. However, the community composition and its functional diversity depending on the habitat have yet to be unveiled. Here, we analyzed the proteomes from litter, plant roots, rhizosphere, and bulk soil in a temperate coniferous forest at two time points to improve the understanding of the interplay between bacterial and eukaryotic communities in different habitats. Our metaproteomic approach yielded a total of 139,127 proteins that allowed to differentiate the contribution of microbial taxa to protein expression as well ...


Distribution, Diversity, And Biogeography Of Anaerobic Carbon Monoxide Uptake By Microbial Communities In Soils And Sediments, Amber N. DePoy 2021 Louisiana State University and Agricultural and Mechanical College

Distribution, Diversity, And Biogeography Of Anaerobic Carbon Monoxide Uptake By Microbial Communities In Soils And Sediments, Amber N. Depoy

LSU Master's Theses

Carbon monoxide (CO) is primarily known for being a toxic gas. However, CO is used by microorganisms as an electron or carbon source in a variety of respiratory processes. Different kinds of microorganisms utilize CO aerobically and anaerobically, using two distinct CO dehydrogenases (CODHs). Aerobes oxidize CO using a molybdenum-dependent dehydrogenase (Mo-CODH), while anaerobes utilize a nickel-dependent CO dehydrogenase (Ni-CODH). Studies of the biochemistry and microbiology of aerobic and anaerobic CO oxidation are extensive, but relatively little is known about the ecology of anaerobic CO oxidation. In an effort to test new hypotheses about the ecology of anaerobic CO oxidation ...


How Historic Shipwrecks Influence Dispersal Of Deep-Sea Microbiomes, Rachel Moseley 2021 The University of Southern Mississippi

How Historic Shipwrecks Influence Dispersal Of Deep-Sea Microbiomes, Rachel Moseley

Master's Theses

This thesis investigates how historic shipwrecks potentially shape dispersal of deep-sea microbiomes. Processes impacting dispersal of microbiomes around artificial structures in the ocean and how they connect to other deep-sea habitats is unknown. Dispersal processes are explained for some macroorganisms by theories in ecology, specifically the Theory of Island Biogeography and the Wooden Stepping Stone hypothesis. These have not been investigated for microorganisms, and thus this work will probe if they are applicable to microbial biogeography in this habitat. Experiments were conducted to establish new “island-like” features in near proximity (25-125 m) to wooden-hulled historic shipwrecks in the northern Gulf ...


A Comparison Of Intertidal Metazoan Biodiversity Between Previously Oiled Sheared And Intact Marsh Margins And Between Multiple Salinity Zones In The Coastal Marshes Of Louisiana, Patrick M. Rayle 2021 Louisiana State University and Agricultural and Mechanical College

A Comparison Of Intertidal Metazoan Biodiversity Between Previously Oiled Sheared And Intact Marsh Margins And Between Multiple Salinity Zones In The Coastal Marshes Of Louisiana, Patrick M. Rayle

LSU Master's Theses

Marshes in Louisiana are under threat from numerous natural and anthropogenic sources. A consequence of these threats are sheared marsh margins, which result from the impact of storm surge on previously oiled, weakened marsh. These conditions occurred in Louisiana marshes after Hurricane Isaac in 2012 followed the Deepwater Horizon oil spill in 2010, particularly in the shorelines surrounding Bay Jimmy. The second and third chapters of this thesis focus on the differences in biodiversity between the sheared and intact marsh margins in impacted sites in Bay Jimmy. Metabarcoding methods were used to determine community composition of the sediment within marsh ...


Disproportionate Ch4 Sink Strength From An Endemic, Sub-Alpine Australian Soil Microbial Community, Marshall D. McDaniel, Marcela Hernández, Marc G. Dumont, Lachlan J. Ingram, Mark A. Adams 2021 Iowa State University

Disproportionate Ch4 Sink Strength From An Endemic, Sub-Alpine Australian Soil Microbial Community, Marshall D. Mcdaniel, Marcela Hernández, Marc G. Dumont, Lachlan J. Ingram, Mark A. Adams

Agronomy Publications

Soil-to-atmosphere methane (CH4) fluxes are dependent on opposing microbial processes of production and consumption. Here we use a soil–vegetation gradient in an Australian sub-alpine ecosystem to examine links between composition of soil microbial communities, and the fluxes of greenhouse gases they regulate. For each soil/vegetation type (forest, grassland, and bog), we measured carbon dioxide (CO2) and CH4 fluxes and their production/consumption at 5 cm intervals to a depth of 30 cm. All soils were sources of CO2, ranging from 49 to 93 mg CO2 m−2 h−1. Forest soils were strong ...


Microbiological Study In A Gneissic Cave From Sri Lanka, With Special Focus On Potential Antimicrobial Activities, Ethige Isuru P. Silva, Pathmakumara Jayasingha, Saman Senanayake, Anura Dandeniya, Dona Helani Munasinghe 2021 University of Sri Jayewardenepura, Sri Lanka

Microbiological Study In A Gneissic Cave From Sri Lanka, With Special Focus On Potential Antimicrobial Activities, Ethige Isuru P. Silva, Pathmakumara Jayasingha, Saman Senanayake, Anura Dandeniya, Dona Helani Munasinghe

International Journal of Speleology

The emergence of antibiotic resistance is a global health crisis, thus the search for novel antimicrobial compounds has become a continuous necessity. Underexplored and extreme environments, such as cave ecosystems, have been identified as a promising potential source for the discovery of novel microorganisms with novel antimicrobial compounds (AMC). This study presents the first cave microbiological investigation in Sri Lanka, with a special preference for bioprospecting of novel AMC. The cave sediment characterization demonstrated the presence of close to strong acidic conditions (pH 3.1 – 3.3) and thus indicates the possibility of isolating acidophilic microorganisms. Eight cave wall/ceiling ...


Evaluation Of Humic Fertilizers Applied At Full And Reduced Nitrogen Rates On Kentucky Bluegrass Quality And Soil Health, Alex J. Lindsey, Adam W. Thoms, Marshall D. McDaniel, Nick E. Christians 2021 Iowa State University

Evaluation Of Humic Fertilizers Applied At Full And Reduced Nitrogen Rates On Kentucky Bluegrass Quality And Soil Health, Alex J. Lindsey, Adam W. Thoms, Marshall D. Mcdaniel, Nick E. Christians

Agronomy Publications

Soil health and sustainable management practices have garnered much interest within the turfgrass industry. Among the many practices that enhance soil health and sustainability are applying soil additives to enhance soil biological activity and reducing nitrogen (N) inputs—complimentary practices. A two-year study was conducted to investigate if reduced N fertilizer rates applied with humic substances could provide comparable turfgrass quality as full N rates, and whether humic fertilizers would increase biological aspects of soil health (i.e., microbial biomass and activity). Treatments included synthetic fertilizer with black gypsum (SFBG), poly-coated humic-coated urea (PCHCU; two rates), urea + humic dispersing granules ...


Metabolomics-Based Analysis Of Miniature Flask Contents Identifies Tobacco Mixture Use Among The Ancient Maya, Mario Zimmermann, Korey J. Brownstein, Luis Pantoja Díaz, Iliana Ancona Aragón, Scott R. Hutson, Barry Kidder, Shannon Tushingham, David R. Gang 2021 Washington State University

Metabolomics-Based Analysis Of Miniature Flask Contents Identifies Tobacco Mixture Use Among The Ancient Maya, Mario Zimmermann, Korey J. Brownstein, Luis Pantoja Díaz, Iliana Ancona Aragón, Scott R. Hutson, Barry Kidder, Shannon Tushingham, David R. Gang

Anthropology Faculty Publications

A particular type of miniature ceramic vessel locally known as “veneneras” is occasionally found during archaeological excavations in the Maya Area. To date, only one study of a collection of such containers successfully identified organic residues through coupled chromatography–mass spectrometry methods. That study identified traces of nicotine likely associated with tobacco. Here we present a more complete picture by analyzing a suite of possible complementary ingredients in tobacco mixtures across a collection of 14 miniature vessels. The collection includes four different vessel forms and allows for the comparison of specimens which had previously formed part of museum exhibitions with ...


Assessment Of Chlorine Resistance Enterobacter Cloacae Isolated From Water Storage Tanks In Sulaimaniyah City-Iraq, Hastyar H. Najmuldeen 2021 Department of Biology, College of Science, University of Sulaimani, Kurdistan Region, Iraq

Assessment Of Chlorine Resistance Enterobacter Cloacae Isolated From Water Storage Tanks In Sulaimaniyah City-Iraq, Hastyar H. Najmuldeen

Passer Journal

This study was designed to determine the extent of contamination of water storage tanks by non-lactose fermenter Enterobacter spp, and to characterize the chlorine and antibiotic resistance status. Moreover, to find the correlation between biofilm formation and resistance to chlorine. For this purpose, a total of 60 water samples were collected from residential and restaurant water storage tanks. Bacterial analysis and antibiotic susceptibility profiles of the samples were assessed by the Most Probable Number (MPN) and Vitek 2 compact tests, respectively. The biofilm formation was quantified by crystal violet staining method and chlorine resistance test by microdilution technique.

Obtained results ...


Complete Genome Sequence Of Strain Ss-5, A Magnetotactic Gammaproteobacterium Isolated From The Salton Sea, A Shallow, Saline, Endorheic Rift Lake Located On The San Andreas Fault In California, Denis Trubitsyn, Caroline L. Monteil, Corey Geurink, Viviana Morillo Lopez, Luiz Gonzaga Paula De Almeida, Ana Tereza Ribeiro De Vasconcelos, Fernanda Abreu, Dennis A. Bazylinski, Christopher T. Lefevre 2021 Longwood University

Complete Genome Sequence Of Strain Ss-5, A Magnetotactic Gammaproteobacterium Isolated From The Salton Sea, A Shallow, Saline, Endorheic Rift Lake Located On The San Andreas Fault In California, Denis Trubitsyn, Caroline L. Monteil, Corey Geurink, Viviana Morillo Lopez, Luiz Gonzaga Paula De Almeida, Ana Tereza Ribeiro De Vasconcelos, Fernanda Abreu, Dennis A. Bazylinski, Christopher T. Lefevre

Life Sciences Faculty Publications

© 2021 American Society for Microbiology. All rights reserved. We report the 3.7-Mb genome sequence of strain SS-5, a magnetotactic, sulfur-oxidizing rod and member of the family Chromatiaceae of the class Gammaproteobacteria, which biomineralizes membrane-bound, elongated, prismatic octahedral, magnetite nanocrystals. This genome sequence brings further diversity for understanding the origin and evolution of magnetotaxis and magnetosome biomineralization.


The Effect Of An Acidified-Gypsum Mixture On Broiler Litter Urease-Producing Bacteria And Nitrogen Mineralization, Taylor Chapman, Christopher D. Burt, Dave Bachoon, Miguel L. Cabrera, Christopher Horacek 2021 Georgia College & State University

The Effect Of An Acidified-Gypsum Mixture On Broiler Litter Urease-Producing Bacteria And Nitrogen Mineralization, Taylor Chapman, Christopher D. Burt, Dave Bachoon, Miguel L. Cabrera, Christopher Horacek

Graduate Research Posters

Broiler chicken (Gallus gallus domesticus) production is one of the largest agricultural sectors in the state of Georgia. Broiler litter produced by broiler facilities is rich in organic nitrogen (N), specifically urea, which can be mineralized by poultry litter urease-producing bacteria (PLUP) forming ammoniacal-N (NH3/NH4+). A large portion of NH3/NH4+ can be lost as ammonia (NH3) gas, which negatively impacts bird health and profitability. The release of NH3 from litter is strongly influenced by the pH of the litter. Flue-gas desulfurization gypsum (FGDG) has been suggested as a potential amendment to reduce ...


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