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140 full-text articles. Page 4 of 7.

Trpm7 Function In Zebrafish Dopaminergic Neurons, Amanda R. Decker 2015 University of Iowa

Trpm7 Function In Zebrafish Dopaminergic Neurons, Amanda R. Decker

Theses and Dissertations

TRPM7 (Transient Receptor Potential Melastatin-like 7) is an ion channel necessary for the proper development of many cell types. Insight into the precise role of the channel in different cells has been hampered by the lethality of knocking out the gene in model organisms such as the mouse. Here I examine a zebrafish that has a loss-of-function mutation in the gene encoding Trpm7. First, I show that trpm7 is important for the function of developing dopaminergic neurons in the zebrafish. Second, I examine the interaction between trpm7 and the related gene vmat2 in order to develop a cellular mechanism of ...


Endothelin-B Receptor Activation In Astrocytes Regulates The Rate Of Oligodendrocyte Regeneration During Remyelination., Timothy R. Hammond, Brian McEllin, Paul D Morton, Matthew Raymond, Jeff Dupree, Vittorio Gallo 2015 George Washington University

Endothelin-B Receptor Activation In Astrocytes Regulates The Rate Of Oligodendrocyte Regeneration During Remyelination., Timothy R. Hammond, Brian Mcellin, Paul D Morton, Matthew Raymond, Jeff Dupree, Vittorio Gallo

Medicine Faculty Publications

Reactive astrogliosis is an essential and ubiquitous response to CNS injury, but in some cases, aberrant activation of astrocytes and their release of inhibitory signaling molecules can impair endogenous neural repair processes. Our lab previously identified a secreted intercellular signaling molecule, called endothelin-1 (ET-1), which is expressed at high levels by reactive astrocytes in multiple sclerosis (MS) lesions and limits repair by delaying oligodendrocyte progenitor cell (OPC) maturation. However, as ET receptors are widely expressed on neural cells, the cell- and receptor-specific mechanisms of OPC inhibition by ET-1 action remain undefined. Using pharmacological approaches and cell-specific endothelin receptor (EDNR) ablation ...


Chemical-Free Technique To Study The Ultrastructure Of Primary Cilium, Ashraf M. Mohieldin, Wissam A. AbouAlaiwi, Min Gao, Surya M. Nauli 2015 Chapman University

Chemical-Free Technique To Study The Ultrastructure Of Primary Cilium, Ashraf M. Mohieldin, Wissam A. Aboualaiwi, Min Gao, Surya M. Nauli

Pharmacy Faculty Articles and Research

A primary cilium is a hair-like structure with a width of approximately 200 nm. Over the past few decades, the main challenge in the study of the ultrastructure of cilia has been the high sensitivity of cilia to chemical fixation, which is required for many imaging techniques. In this report, we demonstrate a combined high-pressure freezing (HPF) and freeze-fracture transmission electron microscopy (FFTEM) technique to examine the ultrastructure of a cilium. Our objective is to develop an optimal high-resolution imaging approach that preserves cilia structures in their best natural form without alteration of cilia morphology by chemical fixation interference. Our ...


Tattletales And T-Bow Update 20151027tue, George McNamara 2015 M.D. Anderson Cancer Center

Tattletales And T-Bow Update 20151027tue, George Mcnamara

George McNamara

20151027Tue this "75"

http://works.bepress.com/gmcnamara/75

is my update of "65" posting

See text at

http://works.bepress.com/gmcnamara/65/

for text summary. The PDf here in "75" supersedes "65".

The PDF here has 10 pages added to the end from the "65" version (pages 40-49 of PDF when including the bepress cover page)..

here is the text in my cover page (bepress may add its own cover):

20151027Tue: added 10 page e-poster at bottom explaining Binary Tattletales and T-Bow. That is, binary with respect to protein components. For one color (number of repeats, epitope tags ...


Dual Roles For An Intracellular Calcium-Signaling Pathway In Regulating Synaptic Homeostasis And Neuronal Excitability, Douglas J Brusich 2015 University of Iowa

Dual Roles For An Intracellular Calcium-Signaling Pathway In Regulating Synaptic Homeostasis And Neuronal Excitability, Douglas J Brusich

Theses and Dissertations

Neurons are specialized cells that communicate via electrical and chemical signaling. It is well-known that homeostatic mechanisms exist to potentiate neuronal output when activity falls. Likewise, while neurons rely on excitable states to function, these same excitable states must be kept in check for stable function. However, the identity of molecular factors and pathways regulating these pathways remain elusive.

Chapter 2 of this thesis reports the findings from an RNA interference- and electrophysiology-based screen to identify factors necessary for the long-term maintenance of homeostatic synaptic potentiation. Data is reported to resolve a long-standing question as to the role of presynaptic ...


Molecular Regulation Of Nox1 Nadph Oxidase In Vascular Smooth Muscle Cell Activation, Jennifer Lee Streeter 2015 University of Iowa

Molecular Regulation Of Nox1 Nadph Oxidase In Vascular Smooth Muscle Cell Activation, Jennifer Lee Streeter

Theses and Dissertations

Nox1 is of considerable importance because of its involvement in a wide variety of pathologies. Activation of Nox1 induces generation of reactive oxygen species (ROS) and cell migration, events critical for the pathogenesis of cardiovascular disease, amyotropic lateral sclerosis, gastrointestinal disease, immunological disorders, and multiple forms of cancer [1-8]. In order to best determine how to treat Nox1-mediated disease, we must gain a better understanding of the mechanisms that control Nox1 activation. Within the last decade, many studies have found that protein phosphorylation and protein trafficking are critical regulatory mechanisms that control the activation of multiple Nox proteins. Yet, to ...


The Justy Mutation Disrupts The Regulation Of Gene Expression And Cell Cycle Progression During B Lymphopoiesis, Jennifer Yamaoka Barr 2015 University of Iowa

The Justy Mutation Disrupts The Regulation Of Gene Expression And Cell Cycle Progression During B Lymphopoiesis, Jennifer Yamaoka Barr

Theses and Dissertations

B lymphopoiesis requires a network of transcription factors that orchestrate changes in gene expression amidst immunoglobulin gene rearrangement and periods of cell proliferation. Although proteins required for the function of this network have been identified, the precise mechanisms that coordinate these processes as hematopoietic progenitors differentiate into lineage-committed B cells remain unclear. Justy mice display a profound arrest of B cell development at the time of lineage commitment due to a point mutation that decreases expression of the protein Gon4-like. Previous studies suggested that Gon4-like functions to coordinate gene expression and cell division to determine cell fate, but the role ...


The Transcription Factor Activator Protein Family Of Genes In Mammary Gland Development And Breast Cancer Progression, Jung Park 2015 University of Iowa

The Transcription Factor Activator Protein Family Of Genes In Mammary Gland Development And Breast Cancer Progression, Jung Park

Theses and Dissertations

Breast cancer is currently the second most common form of cancer and the second leading cause of death due to cancer in the United States. Breast cancer itself is subdivided into at least four subtypes, luminal A, luminal B, HER2-enriched, and basal-like, based on genomewide molecular expression patterns. Luminal A is the most common form and typically characterized by high levels of estrogen receptor (ER). HER2-enriched cancers usually, but not always, harbor amplified copies of the HER2 oncogene. Luminal B cancers share characteristics with the luminal A and HER2-enriched subtypes. Finally, basal-like cancers are more oftentimes defined by their lack ...


Live Imaging Of The Ependymal Cilia In The Lateral Ventricles Of The Mouse Brain, Alzahra J. Al Omran, Hannah C. Saternos, Tongyu Liu, Surya M. Nauli, Wissam A. AbouAlaiwi 2015 University of Toledo

Live Imaging Of The Ependymal Cilia In The Lateral Ventricles Of The Mouse Brain, Alzahra J. Al Omran, Hannah C. Saternos, Tongyu Liu, Surya M. Nauli, Wissam A. Aboualaiwi

Pharmacy Faculty Articles and Research

Multiciliated ependymal cells line the ventricles in the adult brain. Abnormal function or structure of ependymal cilia is associated with various neurological deficits. The current ex vivo live imaging of motile ependymal cilia technique allows for a detailed study of ciliary dynamics following several steps. These steps include: mice euthanasia with carbon dioxide according to protocols of The University of Toledo’s Institutional Animal Care and Use Committee (IACUC); craniectomy followed by brain removal and sagittal brain dissection with a vibratome or sharp blade to obtain very thin sections through the brain lateral ventricles, where the ependymal cilia can be ...


Human Anatomy And Physiology Preparatory Course, Carlos Liachovitzky 2015 CUNY Bronx Community College

Human Anatomy And Physiology Preparatory Course, Carlos Liachovitzky

Open Educational Resources

The overall purpose of this preparatory course textbook is to help students familiarize with some terms and some basic concepts they will find later in the Human Anatomy and Physiology I course.

The organization and functioning of the human organism generally is discussed in terms of different levels of increasing complexity, from the smallest building blocks to the entire body. This Anatomy and Physiology preparatory course covers the foundations on the chemical level, and a basic introduction to cellular level, organ level, and organ system levels. There is also an introduction to homeostasis at the beginning.


Dopaminergic Signaling Within The Primary Cilia In The Renovascular System, Kimberly F. Atkinson, Samred H. Kathem, Xingjian Jin, Brian S. Muntean, Wissam A. AbouAlaiwi, Andromeda M. Nauli, Surya M. Nauli 2015 Chapman University

Dopaminergic Signaling Within The Primary Cilia In The Renovascular System, Kimberly F. Atkinson, Samred H. Kathem, Xingjian Jin, Brian S. Muntean, Wissam A. Aboualaiwi, Andromeda M. Nauli, Surya M. Nauli

Pharmacy Faculty Articles and Research

Activation of dopamine receptor type-5 (DR5) has been known to reduce systemic blood pressure, most likely by increasing renal vasodilation and enhancing natriuresis in the kidney. However, the mechanism of DR5 in natriuresis and vasodilation was not clearly known. We have previously shown that DR5 is localized to primary cilia of proximal renal epithelial and vascular endothelial cells. We here show that selective activation of DR5 specifically induces calcium influx only in the primary cilia, whereas non-selective activation of dopamine receptor induces calcium fluxes in both cilioplasm and cytoplasm. Cilia-independent signaling induced by thrombin only shows calcium signaling within cytoplasm ...


(R)-Desmolactone Is A Sex Pheromone Or Attractant For The Endangered Valley Elderberry Longhorn Beetle Desmocerus Californicus Dimorphus And Several Congeners (Cerambycidae: Lepturinae), A. M. Ray, R. A. Arnold, I. Swift, P. A. Schapker, S. McCann, C. J. Marshall, J. S. McElfresh, J. G. Millar 2014 Xavier University

(R)-Desmolactone Is A Sex Pheromone Or Attractant For The Endangered Valley Elderberry Longhorn Beetle Desmocerus Californicus Dimorphus And Several Congeners (Cerambycidae: Lepturinae), A. M. Ray, R. A. Arnold, I. Swift, P. A. Schapker, S. Mccann, C. J. Marshall, J. S. Mcelfresh, J. G. Millar

Faculty Scholarship

We report here that (4R,9Z)-hexadec-9-en-4-olide [(R)-desmolactone] is a sex attractant or sex pheromone for multiple species and subspecies in the cerambycid genus Desmocerus. This compound was previously identified as a female-produced sex attractant pheromone of Desmocerus californicus californicus. Headspace volatiles from female Desmocerus aureipennis aureipennis contained (R)-desmolactone, and the antennae of adult males of two species responded strongly to synthetic (R)-desmolactone in coupled gas chromatography-electroantennogram analyses. In field bioassays in California, Oregon, and British Columbia, traps baited with synthetic (R)-desmolactone captured males of several Desmocerus species and subspecies. Only male beetles were captured, indicating ...


Regulation Of Peripheral Molecular Clocks In Mammalian Tissues And In Vitro Skeletal Muscle Activation Of Amp-Activated Protein Kinase Via Aicar, Alex C. Lupolt, Daniel P. Moorhead, Josef Brandauer 2014 Gettysburg College

Regulation Of Peripheral Molecular Clocks In Mammalian Tissues And In Vitro Skeletal Muscle Activation Of Amp-Activated Protein Kinase Via Aicar, Alex C. Lupolt, Daniel P. Moorhead, Josef Brandauer

Student Publications

Most organisms possess a common molecular machinery that governs cellular and tissue circadian rhythmicity through a roughly 24-hour transcription-translation feedback loop. It is estimated that up to 15 percent of human genes are influenced by the core clock machinery. It is likely, however, that the metabolic networks affected by the molecular clock differ according to body tissue.

Recent evidence suggests that peripheral molecular clocks are governed to a greater extent by energy availability than by light and dark cycles. AMP-activated protein kinase (AMPK) acts as a cellular fuel gauge within the cell and is activated in response to exercise and ...


N-Linked Glycosylation Of The Bone Morphogenetic Protein Receptor Type 2 (Bmpr2) Enhances Ligand Binding, Jonathan W. Lowery Ph.D., Jose M Amich, Alex Andonian, Vicki Rosen 2014 Marian University - Indianapolis

N-Linked Glycosylation Of The Bone Morphogenetic Protein Receptor Type 2 (Bmpr2) Enhances Ligand Binding, Jonathan W. Lowery Ph.D., Jose M Amich, Alex Andonian, Vicki Rosen

Faculty Publications and Research

The bone morphogenetic protein (BMP) signaling pathway is essential for normal development and tissue homeostasis. BMP signal transduction occurs when ligands interact with a complex of type 1 and type 2 receptors to activate downstream transcription factors. It is well established that a single BMP receptor may bind multiple BMP ligands with varying affinity, and this has been largely attributed to conformation at the amino acid level. However, all three type 2 BMP receptors (BMPR2, ACVR2A/B) contain consensus N-glycosylation sites in their extracellular domains (ECDs), which could play a role in modulating interaction with ligand. Here, we show a ...


Regulation Of Protein Trafficking By Ral Gtpases And Exocyst In Epithelial Cells, Yu-Tsan Liu 2014 University of Iowa

Regulation Of Protein Trafficking By Ral Gtpases And Exocyst In Epithelial Cells, Yu-Tsan Liu

Theses and Dissertations

In polarized epithelial cells, vectorial protein trafficking is important for transporting specific membrane proteins to generate distinct apical and basolateral membrane protein compositions. The Exocyst is a conserved hetero-octameric protein complex, which regulates different aspects of protein trafficking, including tethering of the Golgi-derived vesicles to target membranes. Two of the Exocyst subunits, Sec5 and Exo84, competitively bind to the small GTPases, RalA and RalB, in a GTP-dependent manner. Although Ral GTPases have been proposed to mediate assembly of Exocyst holocomplexes, we hypothesize that they actually serve to allosterically regulate Exocyst functions by promoting association or disassociation of additional factors. Previous ...


The Effect Of Repeated Mild Traumatic Brain Injury On Ventricular Volume And Microglial Activation, Lillian Rose Talbot 2014 University of Connecticut

The Effect Of Repeated Mild Traumatic Brain Injury On Ventricular Volume And Microglial Activation, Lillian Rose Talbot

Honors Scholar Theses

As the leading cause of death and disability in individuals under the age of 45-years-old, Traumatic Brain Injury (TBI) is a public health crisis that demands the attention of the scientific and medical community [28]. The majority of all TBIs that occur in the United States each year are a non-deadly yet detrimental form of closed brain injury known as mild TBI (mTBI) or concussion [6]. Athletes, young people and military personnel all face a high risk of acquiring mTBI as a result of their environments. In our study we have chosen to model repeated mTBI (rmTBI) in the mouse ...


Use Of Zebrafish To Test Candidate Genes And Mutations Associated With Structural Birth Defects, Primarily In Cleft Lip And Palate, Tiffany Lynn Smith 2014 University of Iowa

Use Of Zebrafish To Test Candidate Genes And Mutations Associated With Structural Birth Defects, Primarily In Cleft Lip And Palate, Tiffany Lynn Smith

Theses and Dissertations

Cleft lip and/or palate (CL/P) is a group of congenital birth defect caused by the failure of the lip and/or palate to properly fuse during facial development. This defect occurs in approximately 1:700 live births and is the most second most common developmental defect. Twin studies and evaluation of family history reveals that risk for CL/P is influenced by genetics. However, to date less than half of the heritable risk for CL/P has been ascribed to specific genes.

To identify new genes involved in CL/P, our colleagues, Dr. Manak and Dr. Murray, screened ...


Regulatory Roles Of Facit Collagens Xii And Xiv In Cornea Stromal And Endothelial Development And Function, Chinda Hemmavanh 2014 University of South Florida

Regulatory Roles Of Facit Collagens Xii And Xiv In Cornea Stromal And Endothelial Development And Function, Chinda Hemmavanh

Graduate Theses and Dissertations

Purpose:

Corneal transparency depends upon the precise organization of corneal stromal extracellular matrix and corneal endothelial function. Stromal structure and extracellular matrix organization is responsible for proper refraction of light into the eye. The corneal endothelium is responsible for pumping excess fluid out of the cornea, effectively maintaining corneal hydration and thickness. Corneal transplantation is the current form of treatment for corneal endothelial and stromal dystrophies. The mechanisms controlling stromal collagen fibril packing and organization into orthogonal layers as well as maturation of the endothelium into a fully functioning cellular layer are unknown. Collagens XII and XIV, fibril associated collagens ...


More Fish (1 Of 4), George McNamara 2014 M.D. Anderson Cancer Center

More Fish (1 Of 4), George Mcnamara

George McNamara

More Stellaris FRISH and GPU Deconvolution, 20140313Thur - part 1 of 4

Please see other content in the FISH Imaging section of

http://works.bepress.com/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://works.bepress.com/gmcnamara/43

http://works.bepress.com/gmcnamara/44

http://works.bepress.com/gmcnamara/45

http://works.bepress.com/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


Beyond Race And Place: Distal Sociological Determinants Of Hiv Disparities, J. R. Robbins, M. Buot, J. P. Docena 2014 Xavier University - Cincinnati

Beyond Race And Place: Distal Sociological Determinants Of Hiv Disparities, J. R. Robbins, M. Buot, J. P. Docena

Faculty Scholarship

Informed behavior change as an HIV prevention tool has yielded unequal successes across populations. Despite decades of HIV education, some individuals remain at high risk. The mainstream media often portrays these risk factors as products of race and national borders; however, a rich body of recent literature proposes a host of complex social factors that influence behavior, including, but not limited to: poverty, income inequality, stigmatizing social institutions and health care access. We examined the relationship between numerous social indicators and HIV incidence across eighty large U.S. cities in 1990 and 2000. During this time, major correlating factors included ...


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