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Bacterial Chondronecrosis With Osteomyelitis In Broilers: Genomics, Phylogenomics, And Methods To Detect Specific Pathogens During Outbreaks., Abdulkarim Abdulaziz A. Shwani 2022 University of Arkansas, Fayetteville

Bacterial Chondronecrosis With Osteomyelitis In Broilers: Genomics, Phylogenomics, And Methods To Detect Specific Pathogens During Outbreaks., Abdulkarim Abdulaziz A. Shwani

Graduate Theses and Dissertations

Lameness is a major issue in animal welfare and the broiler industry. Bacterial chondronecrosis with osteomyelitis (BCO) is one of the main causes of lameness. Many staphylococcal species, including Staphylococcus agnetis isolate 908, have been isolated from the bones and blood of lame broilers at the University of Arkansas. Staphylococcus agnetis is a coagulase-variable, Gram-positive bacterial species that has been previously associated with subclinical or mild clinical cases of mastitis in dairy cattle. The annotated complete genome of hypervirulent strain 908 was published at NCBI. In this study, it has been compared to nine genomes we assembled for hypervirulent isolates …


Identification Of Genomic, Proteomic, And Metabolomic Signatures Associated With Pulmonary Hypertension Syndrome In Broilers, Duaa Almansaf 2022 University of Arkansas, Fayetteville

Identification Of Genomic, Proteomic, And Metabolomic Signatures Associated With Pulmonary Hypertension Syndrome In Broilers, Duaa Almansaf

Graduate Theses and Dissertations

The present dissertation contains a collection of studies that examine the genomic, proteomic, and metabolomic association to pulmonary hypertension or ascites phenotype in fast-growing broilers. Pulmonary hypertension is a multifactorial metabolic disease influenced by physiological, environmental, and nutritional factors. It is characterized by a number of structural changes including, thrombosis and adverse pulmonary vascular remodeling. Thus, the atrial pressure is increased, and the right ventricle becomes hypertrophied, resulting in heart failure and the death of the bird. Pulmonary hypertension or ascites is a global problem that has negatively impacted the economy. The increased mortality rate of broilers (25%) is estimated …


A Review Of Heat Stress In Chickens. Part Ii: Insights Into Protein And Energy Utilization And Feeding, Jean-Remi Teyssier, Giorgio Brugaletta, Federico Sirri, Sami Dridi, Samuel J. Rochell 2022 University of Arkansas, Fayetteville

A Review Of Heat Stress In Chickens. Part Ii: Insights Into Protein And Energy Utilization And Feeding, Jean-Remi Teyssier, Giorgio Brugaletta, Federico Sirri, Sami Dridi, Samuel J. Rochell

Poultry Science Faculty Publications and Presentations

With the growing global demand for animal protein and rising temperatures caused by climate change, heat stress (HS) is one of the main emerging environmental challenges for the poultry industry. Commercially-reared birds are particularly sensitive to hot temperatures, so adopting production systems that mitigate the adverse effects of HS on bird performance is essential and requires a holistic approach. Feeding and nutrition can play important roles in limiting the heat load on birds; therefore, this review aims to describe the effects of HS on feed intake (FI) and nutrient digestibility and to highlight feeding strategies and nutritional solutions to potentially …


Recruitment, Cost Indexes, And Management Of Box-Nesting Wood Ducks In South Carolina And North Carolina, Emily Miller 2022 Clemson University

Recruitment, Cost Indexes, And Management Of Box-Nesting Wood Ducks In South Carolina And North Carolina, Emily Miller

All Theses

The wood duck (Aix sponsa) has experienced one of the most significant declines and recoveries among species of North American waterfowl (Anatidae). With enactment of the Migratory Bird Treaty Act (1918) and installation of hundreds of thousands of artificial nest structures for this cavity-nesting species in North America, wood duck populations have recovered and remain a sustainable harvested resource. However, long-term research on box-nesting wood ducks conducted at the Savannah River Ecology Laboratory, Aiken, South Carolina, has revealed uncertainty as to whether recruitment rates of yearling females from natal box-nesting populations are self-sustaining without immigration of hens from …


Intestinal Microbiota Analysis Of Broiler Chickens Under Necrotic Enteritis Challenge And Tributyrin Supplementation, Taylor Nicole McKinney 2022 University of Arkansas, Fayetteville

Intestinal Microbiota Analysis Of Broiler Chickens Under Necrotic Enteritis Challenge And Tributyrin Supplementation, Taylor Nicole Mckinney

Graduate Theses and Dissertations

Poultry is a staple protein source for most of the planet. Until recently, antibiotic growth promoters (AGPs) were used to prevent illnesses in commercial chicken production. Currently, this is not possible due to regulations and consumer concern, but without such a preventative, diseases like necrotic enteritis (NE) have reemerged, posing a threat to bird health, and ultimately, our food source. Necrotic enteritis is a severe gastrointestinal disease caused by the gram-positive pathogen, Clostridium perfringens. Clinical features of this disease are diarrhea, intestinal lesions, and death, with a high transmission rate. In a subclinical form, growth performance is diminished and is …


The Role Of Microbiota On Campylobacter Jejuni Colonization And Growth Performance In Broiler Chickens, Ayidh Almansour 2022 University of Arkansas, Fayetteville

The Role Of Microbiota On Campylobacter Jejuni Colonization And Growth Performance In Broiler Chickens, Ayidh Almansour

Graduate Theses and Dissertations

The successful poultry production at modern era comes from the vertical integratedindustrialization, which has fundamentally changed how animals have been living for millions of years. Antimicrobial growth promoters have been used to sustain the efficient industrialized animal production, driving antimicrobial overuse and resistance. Because of the increasing pressure from consumer’s concerns and government regulations, it is urgent to develop antimicrobial free alternatives as growth promoters in poultry production, but few effective antimicrobial alternatives are currently available. Campylobacter jejuni is one of the worldwide prevalent foodborne bacterial pathogens mainly transmitted from poultry. However, few mechanisms are available on why C. jejuni …


Chondronecrosis With Osteomyelitis In Broilers: Bacterial Species Involvement And Influence Of Probiotics And Synbiotics, Amer Abdullah Hasan 2022 University of Arkansas, Fayetteville

Chondronecrosis With Osteomyelitis In Broilers: Bacterial Species Involvement And Influence Of Probiotics And Synbiotics, Amer Abdullah Hasan

Graduate Theses and Dissertations

Since 1945, the market for broiler production has evolved from a collection of smallholder poultry farms into an industry worth billions of dollars. Poultry production has not gone without challenges. Lameness in young broilers is presently a serious issue in the poultry industry for reasons of animal health and welfare, as well as significant lost production and revenue. Bacterial chondronecrosis with osteomyelitis (BCO) is a major form of lameness in broilers. This dissertation contains information about (i) the influence of Staphylococcus agnetis, Staphylococcus saprophyticus, Staphylococcus epidermidis and Enterococcus faecalis on inducing BCO lameness, (ii) evaluation of of probiotics for reducing …


The Role Of Temperature Variation On Macrophage Physiology And Function In Vitro In Mice, Chicken, And Fish, Intisar Abdullah Hassan 2022 University of Arkansas, Fayetteville

The Role Of Temperature Variation On Macrophage Physiology And Function In Vitro In Mice, Chicken, And Fish, Intisar Abdullah Hassan

Graduate Theses and Dissertations

Fever is an essential component of the immune response. Baseline body temperatures vary in different species, and fever represents increased temperature over baseline. Fever initiates and enhances immune responses as well as creates an environment in which the body has advantages over pathogens. Macrophages are often the first line of cells that come in contact with pathogens, asthey reside in and traffic through tissues. They are important for their engulfment of pathogens that results in the digestion of the pathogen, and they also produce nitric oxide and cytokines that contribute to immune responses in a variety of ways, including initiating …


Histomonosis In Turkeys – Factors Influencing Transmission, Pathogenesis, And The Search For Prophylactic Or Therapeutic Compounds, Thainá Landim de Barros 2022 University of Arkansas, Fayetteville

Histomonosis In Turkeys – Factors Influencing Transmission, Pathogenesis, And The Search For Prophylactic Or Therapeutic Compounds, Thainá Landim De Barros

Graduate Theses and Dissertations

The incidence of histomonosis has been increasing in poultry since the ban of prophylactic and therapeutic compounds. Histomonosis is caused by the protozoa Histomonas meleagridis. The objective of this dissertation was to investigate factors impacting the pathogenesis and transmission of histomonosis and to evaluate compounds that could potentially prevent or reduce the severity of histomonosis in turkeys. In the first study, the effect of sodium chlorate and sodium nitrate on reducing histomonads growth was tested in vitro and added to a basal turkey diet. A decrease in the growth of histomonads in vitro was observed, but no in vivo effect …


Remembering Lanny Randolph, William Flack 2022 University of Nebraska - Lincoln

Remembering Lanny Randolph, William Flack

Nebraska Bird Review

Nebraska's birding community suffered a great loss on June 17, 2022, with the death of Lanny Randolph, an enthusiastic birder and a good friend to all of us. ... Lanny ... resolutely continued birding. In May 2022, he was able to attend the NOU meeting in Gering, and to see many of his longtime friends for the first time since the COVID pandemic. But a month later, on June 17, he was taken from us. Lanny's no longer physically present among us. But for those of us who knew him, he'll always be there in our memories, an invisible presence …


Spring Field Report, March-May 2022, W. Ross Silcock 2022 Nebraska Ornithological Union

Spring Field Report, March-May 2022, W. Ross Silcock

Nebraska Bird Review

This spring there was a large number of interesting reports generated by active field observers, particularly in the west, where our knowledge continues to be broadened in all aspects. The main takeaway this spring was the inordinate plethora of species that matched or exceeded early arrival dates listed in BONO. There were no fewer than 70 species in this category, involving most families and genera, suggesting some overall influence on migration timing. Perhaps exceptions to these early arrivals were some lingering waterfowl and gulls although terns arrived early. Fully 50 of the early arrivers were passerines, notably including nine species …


Remembering Rosalind Morris, 2022 University of Nebraska - Lincoln

Remembering Rosalind Morris

Nebraska Bird Review

Longtime NOU member Mary Rosalind Morris passed away in Lincoln on March 26, 2022, at the age of 101. ... Her membership in the NOU spanned decades, and she held the offices of vice president (1960), president (1975), librarian (1988-1989) and NBR editor (1992-1997). She was also a member of the Friends of Wilderness Park, Lincoln Naturalist Club, Wachiska Audubon, and the Nature Conservancy. For many years while on walks with her dog she picked up litter in Lincoln parks and on campus and received several environmental stewardship awards for her efforts. She is remembered by those who knew her …


Nou Spring Field Days And Annual Meeting, Gering, May 20-22, 2022, Janis Paseka 2022 Nebraska Ornithologists' Union

Nou Spring Field Days And Annual Meeting, Gering, May 20-22, 2022, Janis Paseka

Nebraska Bird Review

The NOU Annual Meeting and Spring Field Days, organized by Kathy DeLara, Robin Harding, Jan Johnson, Alice Kenitz and Ruthie Stearns, was held in Gering on May 20-22, 2022, with 64 in attendance. This was the first time the organization had been able to get together since the meeting in Imperial in September of 2019. Our spring and fall meetings in 2020 and 2021 were cancelled due to the COVID-19 pandemic. Our meetings and meals took place in the Gering Civic Center. The weekend weather varied from cool and sunny to windy, snowy, or rainy, but the total species list …


Duodenal Metabolic Profile Changes In Heat-Stressed Broilers, Jalila S. Dridi, Elizabeth Greene, Craig W. Maynard, Giorgio Brugaletta, Alison Ramser, Courtney J. Christopher, Shawn R. Campagna, Hector F. Castro, Sami Dridi 2022 Université d’Orléans

Duodenal Metabolic Profile Changes In Heat-Stressed Broilers, Jalila S. Dridi, Elizabeth Greene, Craig W. Maynard, Giorgio Brugaletta, Alison Ramser, Courtney J. Christopher, Shawn R. Campagna, Hector F. Castro, Sami Dridi

Poultry Science Faculty Publications and Presentations

Heat stress (HS) is devastating to poultry production sustainability worldwide. In addition to its adverse effects on growth, welfare, meat quality, and mortality, HS alters the gut integrity, leading to dysbiosis and leaky gut syndrome; however, the underlying mechanisms are not fully defined. Here, we used a high-throughput mass spectrometric metabolomics approach to probe the metabolite profile in the duodenum of modern broilers exposed to acute (AHS, 2 h) or chronic cyclic (CHS, 8 h/day for 2 weeks) HS in comparison with thermoneutral (TN) and pair-fed birds. Ultra high performance liquid chromatography coupled with high resolution mass spectrometry (UHPLC–HRMS) identified …


Feed Composition And Isolate Of Histomonas Meleagridis Alter Horizontal Transmission Of Histomonosis In Turkeys. Proof Of Concept, Thaina L. Barros, Christine N. Vuong, Juan D. Latorre, Roberto S. Cuesta, Elizabeth McGill, Samuel J. Rochell, Guillermo Tellez-Isaias, Billy Hargis 2022 University of Arkansas, Fayetteville

Feed Composition And Isolate Of Histomonas Meleagridis Alter Horizontal Transmission Of Histomonosis In Turkeys. Proof Of Concept, Thaina L. Barros, Christine N. Vuong, Juan D. Latorre, Roberto S. Cuesta, Elizabeth Mcgill, Samuel J. Rochell, Guillermo Tellez-Isaias, Billy Hargis

Poultry Science Faculty Publications and Presentations

Outbreaks of histomonosis in turkeys are typically initiated by the ingestion of contaminated embryonated eggs of Heterakis gallinarum, potentially present in earthworms and mechanical vectors. Once an outbreak is started, infected turkeys can transmit the disease by horizontal transmission. Factors influencing horizontal transmission of histomonosis are poorly understood. Replication of horizontal transmission in experimental conditions has not been consistent, presenting an obstacle in searching for alternatives to prevent or treat the disease. Two pilot experiments and three validation experiments were conducted in the present study. In pilot experiment 1, one isolate of Histomonas meleagridis (named Buford) was used. Turkeys …


Interactions Of The Branched-Chain Amino Acids. 2. Practical Adjustments In Valine And Isoleucine, C. W. Maynard, G. J. Mullenix, C. J. Maynard, J. T. Lee, S. K. Rao, L. D. Butler, S. K. Orlowski, M. T. Kidd 2022 University of Arkansas, Fayetteville

Interactions Of The Branched-Chain Amino Acids. 2. Practical Adjustments In Valine And Isoleucine, C. W. Maynard, G. J. Mullenix, C. J. Maynard, J. T. Lee, S. K. Rao, L. D. Butler, S. K. Orlowski, M. T. Kidd

Poultry Science Faculty Publications and Presentations

Reductions in dietary crude protein have potentially allowed for the occurrence of branched chain amino acid antagonism which can negatively influence live performance and carcass traits. Therefore, 2 factorial studies were conducted with the first experiment evaluating valine and leucine interactions during a 32 to 45 d finisher period, while the second experiment evaluated valine and isoleucine interactions during a 39 to 52 d withdrawal period. Valine, leucine, nor their subsequent interaction influenced broiler live performance or carcass traits in Experiment 1. However, a valine x leucine interaction was observed for average woody breast score and occurrence of scores of …


Titration Of Dietary Isoleucine And Evaluation Of Branched-Chain Amino Acid Levels In Female Cobb 500 Broilers During A 22-To 42-Day Finisher Period, C. W. Maynard, G. J. Mullenix, C. J. Maynard, S. C. Wells-Crafton, J. T. Lee, S. K. Rao, L. D. Butler, S. K. Orlowski, M. T. Kidd 2022 University of Arkansas, Fayetteville

Titration Of Dietary Isoleucine And Evaluation Of Branched-Chain Amino Acid Levels In Female Cobb 500 Broilers During A 22-To 42-Day Finisher Period, C. W. Maynard, G. J. Mullenix, C. J. Maynard, S. C. Wells-Crafton, J. T. Lee, S. K. Rao, L. D. Butler, S. K. Orlowski, M. T. Kidd

Poultry Science Faculty Publications and Presentations

Recent research into the branched-chain amino acids has indicated that broiler responses to dietary isoleucine may vary from classic data. Current isoleucine data has focused on male broilers leaving a gap in the literature for female data. An experiment was conducted to determine the isoleucine requirements for a 22 to 42 day finisher period in Cobb MV & POUND; 500 female broilers. A basal diet was formulated to contain dietary isoleucine, valine, and leucine to lysine ratios of 56, 78, and 135, respectively, and graded amounts of L-isoleucine were added to achieve isoleucine to lysine ratios of 56, 59, 62, …


Interactions Of The Branched-Chain Amino Acids. 1. Influence Of Dietary Isoleucine And Leucine On The Valine Requirement Of Male Cobb Mv X 500 Broilers For A 29-To 42-Day Finisher Period, C. W. Maynard, G. J. Mullenix, C. J. Maynard, J. T. Lee, J. Caldas, J. Z. Hiltz, S. K. Orlowski, M. T. Kidd 2022 University of Arkansas, Fayetteville

Interactions Of The Branched-Chain Amino Acids. 1. Influence Of Dietary Isoleucine And Leucine On The Valine Requirement Of Male Cobb Mv X 500 Broilers For A 29-To 42-Day Finisher Period, C. W. Maynard, G. J. Mullenix, C. J. Maynard, J. T. Lee, J. Caldas, J. Z. Hiltz, S. K. Orlowski, M. T. Kidd

Poultry Science Faculty Publications and Presentations

Branched-chain amino acid requirements have traditionally been determined using dose-response titrations without accounting for the influence of branched-chain amino acid antagonism. As recent data has indicated that inherent levels of the non-tested amino acid may influence response curves in diets mimicking industry, these methods may result in over or under estimation of requirement values. Therefore, an experiment was conducted to determine if dietary levels of isoleucine and leucine influence 29 to 42 day valine needs through the implementation of a 5 x 2 x 2 factorial design. Twenty diets were fed to 7 replicate pens of 12 broilers. Body weight …


Research Note: Virulence Gene Downregulation And Reduced Intestinal Colonization Of Salmonella Enterica Serovar Typhimurium Phl2020 Isolate In Broilers By A Natural Antimicrobial (Neutrapath™), H. Hue, D. Wang, B. M. Hargis, G. Tellez-Isaias 2022 Amlan International

Research Note: Virulence Gene Downregulation And Reduced Intestinal Colonization Of Salmonella Enterica Serovar Typhimurium Phl2020 Isolate In Broilers By A Natural Antimicrobial (Neutrapath™), H. Hue, D. Wang, B. M. Hargis, G. Tellez-Isaias

Poultry Science Faculty Publications and Presentations

The reduction in antibiotic growth promoter use in poultry, due to antibiotic resistance concerns, has created a need for natural solutions that control enteric pathogens like Salmonella. One of these natural feed additives, a select blend of essential oils, fatty acids, and an enterosorbent mineral (NeutraPath), was assessed for its effects on the intestinal colonization of Salmonella enterica serovar Typhimurium PHL2020 isolate (ST-PHL2020) in broiler chickens and ST-PHL2020 virulence gene expression. An in vitro digestion model simulating the pH and enzymatic conditions of 3 gastrointestinal compartments (crop, proventriculus, and intestine) was first used to evaluate the antibacterial effects of …


Failure Of Excess Leucine To Impact Live Performance And Carcass Traits In Male And Female Cobb Mv X 500 Broilers During A 15-To 32-Day Grower Period, C. W. Maynard, G. J. Mullenix, C. J. Maynard, J. T. Lee, S. K. Rao, L. D. Butler, J. Z. Hiltz, S. K. Orlowski, M. T. Kidd 2022 University of Arkansas, Fayetteville

Failure Of Excess Leucine To Impact Live Performance And Carcass Traits In Male And Female Cobb Mv X 500 Broilers During A 15-To 32-Day Grower Period, C. W. Maynard, G. J. Mullenix, C. J. Maynard, J. T. Lee, S. K. Rao, L. D. Butler, J. Z. Hiltz, S. K. Orlowski, M. T. Kidd

Poultry Science Faculty Publications and Presentations

Modern broiler diets may contain excess levels of leucine due to the increased level of this amino acid in cereal grains. Excessive levels of leucine have previously been shown to negatively influence broiler performance due to branched-chain amino acid antagonism. Therefore, 2 leucine titrations were conducted in male and female Cobb 500 broilers. Low and high leucine diets were formulated to contain leucine to lysine ratios of 115 and 170, respectively. These diets were subsequently blended to produce 6 experimental diets containing leucine to lysine ratios of: 115, 126, 137, 148, 159, and 170. Pelleted diets were fed to 8 …


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