Biological Engineering Commons^™

Protecting Crops From Abiotic Stress: Copper Oxide Nanoparticle Effects On Wheat And A Beneficial Rhizobacterium, Matthew Potter

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nanoparticles (NPs) are defined as particles less than 100 nm in at least one dimension. CuO NPs have possible applications in agriculture as micronutrient sources, pesticides, and enhancers of crop stress tolerance. Here, three aspects of CuO NP agricultural applications are studied: 1) the potential of CuO NPs to prevent wheat lodging-when crops irreversibly fall over; 2) CuO NP-induced drought tolerance in wheat seedlings; and 3) the effects of CuO NPs on outer membrane vesicle (OMV) production by Pseudomonas chlororaphis O6 (PcO6), a plant-health promoting bacterium.

Wheat grown 7 d exposed to CuO NPs in the growth matrix …

Photo-Characterization Of Selective Luminescent Protein Aggregate Sensors - Oligo-P-Phenylene Ethynylene (Opes), Florencia A. Monge

Biomedical Engineering ETDs

Neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease,

and related tauopathies, are a global health care challenge due to the lack of early

treatment and diagnostic options. These diseases take an emotional and physical

toll on patients, caretakers, and a rather large economic toll on taxpayer-based

health care systems. The pathologies of these diseases are characterized by the

aggregation of misfolded proteins, amyloid-β (Aβ) and tau, into β-sheet rich

structures called amyloids. Research has found that the presence of protein

aggregates predates cognitive symptoms by years. Current diagnostic tools are

based on the detection of these amyloid aggregates, historically by …

Polymeric Nanoparticles As An Antioxidant Delivery System For Age-Related Eye Disease, Sean M. Swetledge

LSU Doctoral Dissertations

Advantages of polymeric nanoparticles as ocular drug delivery systems include controlled release, enhanced drug stability and bioavailability, and specific tissue targeting. Nanoparticle properties such as hydrophobicity, size, and charge, mucoadhesion, as well as administration route and suspension media affect their ability to overcome ocular barriers and distribute in the eye, and must be carefully designed for specific target tissues and ocular diseases. A review was conducted to serve as a guide to optimizing polymeric nanoparticle delivery systems for ocular drug delivery by discussing the effects of nanoparticle composition and administration method on their ocular penetration, distribution, elimination, toxicity, and efficacy, …

Discovery Of A Novel Analogue Of Fr901533 And The Corresponding Biosynthetic Gene Cluster From Streptosporangium Roseum No. 79089, Fuchao Xu, Yonghong Liang, Jie Ren, Siyuan Wang, Jixun Zhan

Biological Engineering Faculty Publications

FR901533 (1, also known as WS79089B), WS79089A (2), and WS79089C (3) are polycyclic aromatic natural products with promising inhibitory activity to endothelin-converting enzymes. In this work, we isolated five tridecaketide products from Streptosporangium roseum No. 79089, including 1-3, benaphthamycin (4) and a novel FR901533 analogue (5). The structure of 5 was characterized based on spectroscopic data. Compared to the major product 2, the new compound 5 has an additional hydroxyl group at C-12 and an extra methyl group at the 13-OH. The configuration of C-19 of these compounds …

Existing Empirical Kinetic Models In Biochemical Methane Potential (Bmp) Testing, Their Selection And Numerical Solution, Yehor Pererva, Charles D. Miller, Ronald C. Sims

Biological Engineering Faculty Publications

Biochemical Methane Potential (BMP) tests are a crucial part of feasibility studies to estimate energy recovery opportunities from organic wastes and wastewater. Despite the large number of publications dedicated to BMP testing and numerous attempts to standardize procedures, there is no “one size fits all” mathematical model to describe biomethane formation kinetic precisely. Importantly, the kinetics models are utilized for treatability estimation and modeling processes for the purpose of scale-up. A numerical computation approach is a widely used method to determine model coefficients, as a replacement for the previously used linearization approach. However, it requires more information for each model …

Bio Circuits For Evolutionary Biotech, Camilo Toruno

ENGS 86 Independent Projects (AB Students)

The field of bioengineering has much promise for renewable chemical production, bioremediation, and of course medical applications. Developing new useful microorganisms is extremely time and capital intensive, typically taking 50 million USD and eight years. This is due in large part to the low throughput techniques that are characteristic of the field of metabolic engineering. Here we describe the modification of an existing synthetic biosensor to measure the pharmaceutical dopamine, and the use of a circuit simulator Cadence to predict improvements to the biosensor. This biosensor paired with directed evolution techniques could reach throughputs of 5 million cells per day …

A Microfluidic Platform For High-Throughput Screening Of Aquaporin Performance, Adriana Del Pino Herrera, Jordan Hoydick, Rachel Rauh, Elyssa El-Hajj, Madison Burchfield

Undergraduate Research and Scholarship Symposium

Aquaporins are a family of small integral membrane proteins that transport water across cell membranes in response to osmotic gradients. They facilitate fluid secretion and absorption across epithelial surfaces in kidney tubules, exocrine glands, and gastrointestinal tract. Here, we describe a novel microfluidic method to evaluate and screen for aquaporin-based transmembrane permeability in mammalian cells. A microfluidic device was designed and fabricated for the encapsulation of single mammalian and yeast cells in micron-sized droplets. For this purpose, Chinese Hamster Ovarian (CHO) cells were used. CHO cells express AQP1 (aquaporin-1) homologous to human kidney aquaporins. The cells were cultivated and exposed …

Pilot Study Exploring The Effect Of Targeted Cox-2 Inhibition In Macrophages Responding To Neuronal Injury; Promoting Enhanced Axonal Regeneration, Alyssa Brauckmann

Electronic Theses and Dissertations

Celecoxib nanoemulsion (CXB-NE) has been developed as a macrophage targeted analgesics by Dr. Janjic and her team at Duquesne University, (Janjic et al, 2018; Liu et al, 2020; Saleem et al, 2019b; Vasudeva et al, 2014). The CXB-NE nanoemulsion carrying a Nonsteroidal Anti-inflammatory (NSAID) inhibitor of COX-2 activity result in a reduction in PGE2 expression in macrophages. Using CXB-NE in rats that have peripheral nerve injury constricting the sciatic nerve relieves hypersensitivity, a pain-like behavior. The treatment also decreases inflammation associated with this chronic constriction injury (Janjic et al, 2018; Saleem et al, 2019b; Stevens et al, 2019). In this …

Design Of A 3d Printed Bioreactor For Bone Cancer Research, Brooklyn K. Vanderwolde, Katelyn Hillson

The Journal of Undergraduate Research

Bone cancer is an aggressive disease and has peak occurrence during physiological stimulation of growth and aging. Astronauts who undergo long-term space missions also acquire an increased risk of bone tissue degeneration and cancer. Few in-vitro models currently exist capable of reproducing the complex microenvironment of bone tissue to support multicellular activity in a three-dimensional structure. This limits the ability to understand disease progression and develop suitable treatment strategies. Therefore, developing a bone tissue model and an associated bioreactor is critical to understand the risks associated with cancer progression and improve treatment and preventions related to those risks. The goal …

Isolation And Selective Glycosylation Of Antisalmonellal Anthraquinones From The Stem Bark Of Morinda Lucida Benth. (Rubiaceae), Napoleon A. Mfonku, James A. Mbah, Norbert Kodjio, Donatien Gatsing, Jixun Zhan

Biological Engineering Faculty Publications

In this work we report the isolation, identification and antibacterial activity of two anthraquinones, 2-hydroxy-1-methoxyanthraquinone (1) and 2,5-dihydroxy-1-methoxy-6-methoxymethylanthraquinone (2), from the stem bark of Morinda lucida. These two natural products were selectively converted into two new glycosylated derivatives, 2-hydroxy-1-methoxyanthraquinone-4′-O-methyl-2-O-β-d-glucopyranoside (3) and 2,5-dihydroxy-1-methoxy-6-methoxymethylanthraquinone-4′-O-methyl-2-O-β-d-glucopyranoside (4) by the filamentous fungus Beauveria bassiana ATCC 7159. Structure elucidation was accomplished based on the 1D and 2D NMR, IR and mass spectra. The glycosylated compounds 3 and 4 showed higher in vitro antibacterial activity against Salmonella enterica subsp. enterica sérovars Typhimurim (MIC of 8 μg/mL each) than the corresponding aglycons 1 and 2 (MIC of …

Cell Line-Specific Differences In Guided Differentiation Of Adipose-Derived Mesenchymal Stem Cells Towards Smooth Muscle Cells, Sherly Makar

Biomedical Engineering Undergraduate Honors Theses

Mesenchymal stem cells (MSCs) are a population of stromal cells found traditionally in the bone marrow and adipose tissues. They can also be found in other tissues including fallopian tube, core blood, peripheral blood, fetal liver, and lungs. Mesenchymal stem cells have profound effects in regenerative engineering, tissue repair and drug discovery owing to the excellent properties such as proliferation, self-renewal, and multipotency generating multiple cell types including adipocytes, osteocytes, cardiomyocytes (CMs), pericytes (PCs), and chondrocytes. MSCs are used as immunomodulators in generating progenitor cells to be used for transplantation, creating engineered organs, and preventing graft vs. host disease (GVHD). …

Oxidative Protection Using Pluronic Micelles, Mitchell Glen Heap

Undergraduate Honors Capstone Projects

Methods for drug delivery are currently being researched in many different applications. One significant obstacle to drug delivery is a drug's inherent hydrophobic character. Drugs that are hydrophobic are difficult to successfully administer since the body consists mostly of water. Therefore, a greater variety of drugs could potentially be used for the treatment of disease if they could successfully be administered. Recent research in oncology and virology has attempted to overcome the obstacles posed by hydrophobic drugs by encapsulating them in micelles. Micelles form spontaneously from compounds that contain both hydrophilic and hydrophobic regions and can be used to increase …

Characterization Of Rhodopseudomonas Palustrus Strains For The Production Of Fixed Nitrogen Fertilizer For Mars, Daniel Kade Derrick

Undergraduate Honors Capstone Projects

The purpose of this project was to characterize the potential of various strains of purple non-sulfur bacteria for the production of fixed nitrogen fertilizers for the manned Mars missions. Six strains of Rhodopseudomonas palustris (CGA009, CGA010, TIE-1, NifA*, and PB23) were all investigated. Through initial growth trial experiments, R. palustris NifA* and PB23 were selected for their engineered nitrogen fixation and rapid growth respectively. Growth curves and ammonium concentrations were collected over time in pilot scale batch photobioreactors (200 mL). Biomass production was then scaled up to benchtop photobioreactors (1.5 L) and then 14 L total volume for both NifA* …

Characterization Of Rhodopseudomonas Palustris Strains For The Production Of Fixed Nitrogen Fertilizer For Mars, Andrew James Walters

Undergraduate Honors Capstone Projects

The purpose of this project was to characterize the potential of various strains of purple non-sulfur bacteria for the production of fixed nitrogen fertilizers for the manned Mars missions. Six strains of Rhodopseudomonas palustris (CGA009, CGA010, TIE-1, NifA*, and PB23) were all investigated. Through initial growth trial experiments, R. palustris NifA* and PB23 were selected for their engineered nitrogen fixation and rapid growth respectively. Growth curves and ammonium concentrations were collected over time in pilot scale batch photobioreactors (200 mL). Biomass production was then scaled up to benchtop photobioreactors (1.5 L). Fourteen liters of both NifA* and PB23 were then …

Comparing Economics Of Traditional Carbonation Method And A Novel Carbonation Invention For Craft Beer, Kira Simonson

Biological and Agricultural Engineering Undergraduate Honors Theses

The traditional method for the carbonation of beer includes bubbling CO₂ through a pressurized brite tank until the desired level of carbonation concentration is reached. The gas either dissolves in the liquid volume or passes through the beer into the gas headspace above the bulk volume of beer. The gas that passes through the liquid can strip the beer of flavors, and this undissolved gas is vented to the atmosphere. To reduce the gas lost to the atmosphere, the CO₂ is dissolved into the beer slowly over a long period of time, which increases gas-use efficiency but sacrifices …

Design Of A Deep Flow Technique Hydroponic System And An Elementary Education Module For Tri Cycle Farms, Cady Rosenbaum

Biological and Agricultural Engineering Undergraduate Honors Theses

Hydroponics is an agricultural technique in which plants are grown without soil and are instead grown in water systems that include nutrients and other growth-supporting media. Hydroponic systems typically reside inside, so that the system can be fully controlled by the grower by manipulating the temperature and amount of light the plants receive. The benefits of growing plants using hydroponics include: the amount of water used is reduced, it is less labor to grow organic produce with an indoor system, less space used, and it allows for growing food anywhere. Tri Cycle Farms is planning for the construction of a …

Development And Validation Of A Synchronous Model To Simulate Skeletal Muscle Damage Due To Spaceflight Ionizing Radiation And Microgravity, Lori M. Caldwell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Astronauts traveling or researching in space are subject to a harsh environment that includes high levels of ionizing radiation and microgravity. Ionizing radiation is dangerous to overall health because it causes double-stranded DNA breaks, increases the production of free radicals or reactive oxygen species, and leads to an enhanced risk of cardiovascular disease and cancer over a person’s lifetime. Similarly, microgravity is hazardous to astronaut health because humans require gravity to maintain a healthy heart and muscular and vascular systems.

Both ionizing radiation and microgravity have been studied individually in previous research, but there is a lack of information on …

Determination Of Gas Emission Characteristics From Animal Wastes Using A Multiplexed Portable Ftir-Surface Chamber System, Pakorn Sutitarnnontr

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Livestock production is a growing source of air pollution at regional, national, and global scales. Improved livestock manure management has the potential to reduce environmental impacts; however, there is an urgent need for cost-efficient, reliable, and easy to maintain measurement and monitoring capabilities to precisely quantify emissions from livestock manure. This research describes and evaluates a novel measurement method based on the multiplexed portable Fourier Transform Infrared (FTIR) spectroscopy analyzer - surface chamber techniques for continuous measurements and monitoring gas emissions from manure sources. The multiplexing system was designed and developed to automate the chamber network, controlling the movement of …

Micro-Biosensor Devices For Biochemical Analysis Applications, Han Zhang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A biosensor is an analytical device integrating a biological element and a physicochemical transducer that convert a biological response into a measurable signal. The advantages of biosensors include low cost, small size, quick, sensitivity and selectivity greater than the conventional instruments. Biosensors have a wide range of applications ranging from clinical diagnostics through to environmental monitoring, agriculture industry, et al.

The different types of biosensors are classified based on the sensor device as well as the biological material. Biosensors can be broadly classified into (piezoelectric, etc.), electrochemical biosensors (potentiometric, amperometric, etc.), and optical types of biosensors (fiber optics, etc.).

Here, …

Observation Of Struvite In The Mixed Microalgae Biofilm Matrix Of A Rotating Algal Biofilm Reactor During Nutrient Removal From Municipal Anaerobic Digester Filtrate, Kyle M. Hillman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Central Valley Water Reclamation Facility (CVWRF) in Salt Lake City is the largest municipal wastewater treatment plant in Utah and must meet new and rigorous nutrient effluent standards–over 95% reduction in phosphorus output by 2025. Filtrate from CVWRF anaerobic digesters contains high levels of nitrogen, phosphorus, and magnesium. Supersaturation of these constituents leads to nuisance struvite precipitation that clogs belts, pumps, and pipes downstream of anaerobic digesters. Struvite is a mineral precipitate composed of equimolar magnesium, ammonium, and phosphate. Controlled precipitation of struvite helps prevent clogging and scaling, removes phosphate from wastewater, and generates a marketable fertilizer product.

Struvite was …

Transcriptomic Analysis Of Cytokine-Treated Tissue-Engineered Cartilage As An In Vitro Model Of Osteoarthritis, Jiehan Li

McKelvey School of Engineering Graduate Student Theses & Dissertations

Osteoarthritis (OA), as the most common form of arthritis and a leading cause of disability worldwide, currently has no disease-modifying drugs. Inflammation plays an important role in cartilage degeneration in OA, and pro-inflammatory cytokines, IL-1β and TNF-α, have been shown to induce degradative changes along with aberrant gene expression in chondrocytes, the only resident cells in cartilage. The goal of this study was to further understand the transcriptomic regulation of tissue-engineered cartilage in response to inflammatory cytokines using an in vitro miPSC model system. We performed RNA sequencing for the IL-1β or TNF-α treated tissue-engineered cartilage derived from murine iPSCs, …

Designing An Antibiotic Eluting Trauma Screw To Reduce Short Term Infection In Bone Fractures, Dominic Lincoln

Honors Theses

Orthopedic infection in the United States is one of the most common and severe health risks faced by trauma patients. The most prevalent infection is orthopedic and trauma device-related infection (ORDI) and often occurs in long bones at the fracture site. The purpose of this paper is to explore this infection, its causes, and solutions currently on the market while providing novel solutions to the problem. The designs proposed will be evaluated for their effectiveness and value, while attempting to keep costs low for all stakeholders. The feasibility of patenting these ideas will also be considered, as will the testing …

Acute Mechanical Stress In Primary Porcine Rpe Cells Induces Angiogenic Factor Expression And In Vitro Angiogenesis, Farhad Farjood, Amir Ahmadpour, Sassan Ostvar, Elizabeth Vargis

Biological Engineering Faculty Publications

Background

Choroidal neovascularization (CNV) is a major cause of blindness in patients with age-related macular degeneration. CNV is characterized by new blood vessel growth and subretinal fluid accumulation, which results in mechanical pressure on retinal pigment epithelial (RPE) cells. The overexpression of RPE-derived angiogenic factors plays an important role in inducing CNV. In this work, we investigated the effect of mechanical stress on the expression of angiogenic factors in porcine RPE cells and determined the impact of conditioned medium on in-vitro angiogenesis.

Results

The goal of this study was to determine whether low levels of acute mechanical stress during early …

Gesea Biosciences - Core Shell Pellet Manufacturing Device, Claire Edelman, Maria Medrano, Rachel Longoria

Biomedical Engineering

No abstract provided.

Methods To Increase Algae Biomass Productivity In Raceway Pond Monocultures, Ryan S. Anderson

Master's Theses

The economics of algae biofuels and bioproducts would be improved by increased biomass productivity. Two studies on this potential are described in this thesis – one on a locally isolated filamentous yellow-green alga and the other on a planktonic strain genetically improved via selective enrichment.

Polycultures have been viewed as productive, stable, and, in some cases, harvestable by natural bioflocculation. Local native strains might have higher productivity than culture collection strains because they are already adapted to local outdoor conditions. In this study, the filamentous yellow-green alga Tribonema minus was isolated from a local volunteer polyculture. Its productivity as a …

Initial Development Of Corn Seedlings After Seed Priming With Nanoscale Synthetic Zinc Oxide, Michel Esper Neto, David W. Britt, Lorena Moreira Lara, Anthony Cartwright, Rayssa Fernanda De Santos, Tadeu Takeyoshi Inoue, Marcelo Augusto Batista

Biological Engineering Faculty Publications

Nanofertilizers are increasingly explored for sustainable micronutrient delivery in agriculture. Pre-treating seeds with nanofertilizers prior to planting (i.e., seed priming) reduces concerns about nanoparticle (NP) fertilizer non-target dispersion; however, priming formulations and concentrations must be carefully selected to avoid germination inhibition and toxicity. Here we investigate changes in corn seed germination and seedling development after seed priming with ZnO NPs, ZnO bulk and ZnCl₂. To evaluate the effects sterile seeds were immersed in priming solutions of 0, 20, 40, 80, 160 mg L⁻¹ Zn for the three Zn sources. Following an 8 h priming the seeds were …

Electrical Characterization And Detection Of Blood Cells And Stones In Urine, Nida Nasir

Dissertations

Urine contains an immense amount of information related to its physical, chemical, and biological components; hence, it is a promising tool in detecting various diseases. Available methods for detecting hematuria (blood in the urine) are not accurate. Results are influenced by many factors, such as, health and vitals of the patients, settings of the equipment and laboratories, which leads to false positive or false negative outputs. This necessitates the development of new, accurate, and easy-access methods that save time and effort. This study demonstrates a label-free and accurate method for detecting the presence of red and white blood cells (RBCs …

"When The Enemy Drew Our Attention": Reconsidering Prior Restraint In The Context Of Dual Use Research Of Concern, Caine Caverly

William & Mary Bill of Rights Journal

Through 2016 and 2017, a team led by Canadian virologist David Evans, and funded by an American pharmaceutical company, attempted to synthesize the previously extinct horsepox virus. After just six months and an expenditure of $100,000, the research team was able to successfully construct the virus “using only commercially available information, technology and tools.” In January of 2018, the team went on to publish their information in an American-based journal, PLOS ONE.

The publication was controversial because it included a potential “blueprint” for the synthesis of a genetic strand in the same viral family as the highly lethal, albeit eradicated, …

Experimental And Computational Tools For Single Cell Analysis In Cancer Diagnostics, Manibarathi Vaithiyanathan

LSU Doctoral Dissertations

Substantial evidence shows that cellular heterogeneity commonly exists within an isogenic or clonal population. Whether in isolation or caused through a combination of the above events, cellular heterogeneity can dramatically influence cellular decision making and cell fate, however, this can be masked by the average response from a population. One approach to solve this issue is to analyze a population at the individual cell level. The goal of this work is to develop high-throughput experimental and computational platforms to screen and quantify single cancer cells for specific intracellular enzyme activities. An interdisciplinary approach was taken to 1) better understand the …

Fluid Dynamics Characterization Of Transcatheter Aortic Valves, Mohammed Barakat

Electronic Theses and Dissertations

Aortic stenosis due to degenerative calcific aortic valvular disease is the most reason for aortic valve replacement in developed countries. Aortic stenosis affects up to 7% of the world population, and current clinical data indicate that the number of the affected people could be triple by 2050, due to population ageing and health lifestyle. Transcatheter aortic valve replacement (TAVR) was introduced as a minimal invasive treatment of severe aortic stenosis. Even though surgical aortic valve replacement (SAVR) is considered the golden standard treatment for severe aortic stenosis patients, TAVR showed equivalent or even superior outcome compare to SAVR. Currently, transcatheter …