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Articles 1 - 22 of 22
Full-Text Articles in Biological Engineering
Genetic Engineering Of Syringyl-Enriched Lignin In Plants, Vincent Lee C. Chiang, Laigeng Li
Genetic Engineering Of Syringyl-Enriched Lignin In Plants, Vincent Lee C. Chiang, Laigeng Li
Michigan Tech Patents
The present invention relates to a novel DNA sequence, which encodes a previously unidentified lignin biosynthetic pathway enzyme, sinapyl alcohol dehydrogenase (SAD) that regulates the biosynthesis of syringyl lignin in plants. Also provided are methods for incorporating this novel SAD gene sequence or substantially similar sequences into a plant genome for genetic engineering of syringyl-enriched lignin in plants.
Odor, H2S And Nh3 Emissions From Phototrophic And Non-Phototrophic Anaerobic Swine Lagoons, Jason Byler, Dennis D. Schulte, Richard K. Koelsch
Odor, H2S And Nh3 Emissions From Phototrophic And Non-Phototrophic Anaerobic Swine Lagoons, Jason Byler, Dennis D. Schulte, Richard K. Koelsch
Department of Biological Systems Engineering: Conference Presentations and White Papers
Odor, ammonia and hydrogen sulfide emission data were collected from three phototrophic and three non-phototrophic anaerobic swine lagoons in eastern Nebraska from May 27th to June 18th (late spring) and from July 7th to August 13th (summer). The greatest odor, hydrogen sulfide and ammonia emission rates were from non-phototrophic lagoons during late spring (24.5 OU m-2 s-1, 3.2 μg-H2S m-2 s-1, 34.9 kg NH3-N ha-1 d-1, respectively). Non-phototrophic lagoon odor, ammonia and hydrogen sulfide emission rates were much higher in late spring than summer (24.5 vs. 4.8 …
On-The-Go Soil Sensors For Precision Agriculture, Viacheslav I. Adamchuk, J. W. Hummel, M. T. Morgan, S. K. Upadhyaya
On-The-Go Soil Sensors For Precision Agriculture, Viacheslav I. Adamchuk, J. W. Hummel, M. T. Morgan, S. K. Upadhyaya
Biological Systems Engineering: Papers and Publications
The basic objectives of site-specific management of agricultural inputs are to increase profitability of crop production, improve product quality, and protect the environment. Information about the variability of different soil attributes within a field is essential for the decision-making process. The inability to obtain soil characteristics rapidly and inexpensively remains one of the biggest limitations of precision agriculture. Numerous researchers and manufacturers have attempted to develop on-the-go soil sensors to measure mechanical, physical and chemical soil properties. The sensors have been based on electrical and electromagnetic, optical and radiometric, mechanical, acoustic, pneumatic, and electrochemical measurement concepts. While only electric and …
Transdermal Delivery Of Phosphorodiamidate Morpholino Oligomers Across Hairless Mouse Skin, Angela K. Pannier, Vikram Arora, Patrick L. Iversen, Rhonda M. Brand
Transdermal Delivery Of Phosphorodiamidate Morpholino Oligomers Across Hairless Mouse Skin, Angela K. Pannier, Vikram Arora, Patrick L. Iversen, Rhonda M. Brand
Biological Systems Engineering: Papers and Publications
The skin is the largest organ in the body and an obvious route for both local and systemic drug delivery. Antisense oligomers have tremendous potential as therapeutic agents for numerous diseases. The objective of this study was to investigate the influence of vehicle on the transdermal delivery of several phosphorodiamidate Morpholino oligomers (PMOs) with different sizes, lengths, base compositions, sequences, and lipophilicities. Eleven different PMOs were synthesized complementary to biologically relevant gene targets and delivered across hairless mouse skin in vitro using vehicles composed of 95% propylene glycol, 5% linoleic acid (PG/LA), water, 50% water:50% PG/LA, and 75% water:25% PG/LA. …
Controlled Release Systems For Non-Viral Vectors, Angela K. Pannier, Lonnie D. Shea
Controlled Release Systems For Non-Viral Vectors, Angela K. Pannier, Lonnie D. Shea
Biological Systems Engineering: Papers and Publications
Adapting controlled release technologies to the delivery of non-viral vectors has the potential to overcome barriers that limit gene therapy. Controlled release systems can enhance gene delivery and increase the extent and duration of transgene expression relative to more traditional delivery methods. Delivery vehicles for controlled release are fabricated from natural and synthetic polymers, which function either by releasing the vector into the local tissue environment or by maintaining the vector at the polymer surface. Vector release or binding is regulated by the effective affinity of the vector for the polymer, which depends upon the strength of molecular interactions. These …
Fatty Acid Composition And Oxidation Of Lipids In Korean Catfish, K. T. Hwang, J. E. Kim, S. G. Kang, S. T. Jung, H. J. Park, Curtis L. Weller
Fatty Acid Composition And Oxidation Of Lipids In Korean Catfish, K. T. Hwang, J. E. Kim, S. G. Kang, S. T. Jung, H. J. Park, Curtis L. Weller
Biological Systems Engineering: Papers and Publications
This study determined the lipid content and FA composition of muscle and a mixture of muscle and viscera from Korean catfish as well as lipid oxidation and hydrolysis. Lipid content and FA compositions in Korean catfish, which were purchased every month or two during September 1999–July 2000, were analyzed. Lipid oxidation and hydrolysis were determined as PV, thiobarbituric acid value, and FFA in the muscle and the mixture during storage at 2°C for 12 d and –14°C for 9 wk. Lipid contents of the muscle and the mixture were 3.2 (w/w) and 5.4%, respectively. Oleic acid was the most abundant …
Total Reduced Sulfur Concentrations In The Vicinity Of Beef Cattle Feedlots, Richard K. Koelsch, Bryan L. Woodbury, David E. Stenberg, Daniel N. Miller, Dennis D. Schulte
Total Reduced Sulfur Concentrations In The Vicinity Of Beef Cattle Feedlots, Richard K. Koelsch, Bryan L. Woodbury, David E. Stenberg, Daniel N. Miller, Dennis D. Schulte
Biological Systems Engineering: Papers and Publications
A field survey of total reduced sulfur (TRS) concentrations in the vicinity of beef cattle feedlots was conducted to compare field observations against current regulatory thresholds. In addition, environmental factors that may contribute to increased TRS emissions were evaluated. It was observed that TRS levels in the vicinity of beef cattle feedlots are not likely to exceed current regulatory thresholds used by midwestern states. It was further noted that concentration of TRS varies with air temperature and time of day. However, wet feedlot surface conditions and wind speed had almost no impact upon observed concentrations.
Bie6300 - Irrigation & Conveyance Control Systems, Spring 2004, Gary P. Merkley
Bie6300 - Irrigation & Conveyance Control Systems, Spring 2004, Gary P. Merkley
Biological and Irrigation Engineering - OCW
This is a course regarding measurement of flow rates and water levels. Calibration, design, and selection of open-channel flow measurement structures. Design of irrigation conveyance and distribution system infrastructure.
Rational Pathway Engineering Of Type I Fatty Acid Synthase Allows The Biosynthesis Of Triacetic Acid Lactone From D-Glucose In Vivo, Wenjuan Zha, Zengyi Shao, John W. Frost, Huimin Zhao
Rational Pathway Engineering Of Type I Fatty Acid Synthase Allows The Biosynthesis Of Triacetic Acid Lactone From D-Glucose In Vivo, Wenjuan Zha, Zengyi Shao, John W. Frost, Huimin Zhao
Zengyi Shao
Metabolic pathway engineering is a powerful tool to synthesize structurally diverse and complex chemicals via genetic manipulation of multistep catalytic systems involved in cell metabolism. Here, we report the rational design of a fatty acid biosynthetic pathway, Brevibacterium ammoniagenes fatty acid synthase B (FAS-B), that allows the microbial synthesis of triacetic acid lactone (TAL) from an inexpensive feedstock, D-glucose. TAL can be chemically converted to phloroglucinol, which is a core structure for the synthesis of various high value bioactive compounds and energetic compounds such as 1,3,5-triamino-2,4,6-trinitrobenzene (TATB). Synthesis of phloroglucinol from d-glucose using this combined biological and chemical synthesis may …
Spatial Analysis Of 3′ Phosphoinositide Signaling In Living Fibroblasts: Ii. Parameter Estimates For Individual Cells From Experiments, Ian C. Schneider, Jason M. Haugh
Spatial Analysis Of 3′ Phosphoinositide Signaling In Living Fibroblasts: Ii. Parameter Estimates For Individual Cells From Experiments, Ian C. Schneider, Jason M. Haugh
Ian C. Schneider
Fibroblast migration is directed by gradients of platelet-derived growth factor (PDGF) during wound healing. As in other chemotactic systems, it has been shown recently that localized stimulation of intracellular phosphoinositide (PI) 3-kinase activity and production of 3′ PI lipids in the plasma membrane are important events in the signaling of spatially biased motility processes. In turn, 3′ PI localization depends on the effective diffusion coefficient, D, and turnover rate constant, k, of these lipids. Here we present a systematic and direct comparison of mathematical model calculations and experimental measurements to estimate the values of the effective 3′ PI diffusion coefficient, …
Changes In Composition And Thermal Transition Temperatures Of Grain Sorghum Wax During Storage, Keum Taek Hwang, Curtis L. Weller, Susan L. Cuppett, Milford Hanna
Changes In Composition And Thermal Transition Temperatures Of Grain Sorghum Wax During Storage, Keum Taek Hwang, Curtis L. Weller, Susan L. Cuppett, Milford Hanna
Biological Systems Engineering: Papers and Publications
Grain sorghum (Sorghum bicolor) wax is composed mainly of aldehydes, alcohols, and acids. Aldehydes, comprising about one-half of the wax, are readily converted to acids in presence of air. In this study, whole sorghum wax and an aldehyde fraction from sorghum wax were subjected to oxidative conditions. Changes in the major components and thermal transition temperatures were determined using HPLC and differential scanning calorimeter (DSC), respectively. The aldehyde fraction was oxidized markedly to acids over 4 months in storage at room temperature. Acid content, in the fraction, was initially 5–7% and increased to 42–51% after 135 days in …
Development Of An Instrumented Deep−Tillage Implement For Sensing Of Soil Mechanical Resistance, Viacheslav I. Adamchuk, Andrey V. Skotnikov, Justin D. Speichinger, Michael F. Kocher
Development Of An Instrumented Deep−Tillage Implement For Sensing Of Soil Mechanical Resistance, Viacheslav I. Adamchuk, Andrey V. Skotnikov, Justin D. Speichinger, Michael F. Kocher
Biological Systems Engineering: Papers and Publications
Variable−depth tillage has the potential for economic and environmental benefits to modern crop production. Varying tillage depth according to local soil conditions prevents the waste of energy and preserves soil ecology. A prototype instrumentation system was developed based on a conventional implement for deep tillage. It was equipped with two load cells and two sets of strain gauges for sensing the load applied to the implement during tillage. Two linear pressure distribution models (full and redundant) were used to describe the change of soil mechanical resistance with depth. These models were then used to compare estimates of soil mechanical resistance …
Measurement And Analyses Of Growth And Stress Parameters Of Viburnum Odoratissimum (Ker-Gawl) Grown In A Multi-Pot Box System, Suat Irmak, Dorota Z. Haman, Ayse Kilic, James W. Jones, Kenneth L. Campbell, Thomas L. Crisman
Measurement And Analyses Of Growth And Stress Parameters Of Viburnum Odoratissimum (Ker-Gawl) Grown In A Multi-Pot Box System, Suat Irmak, Dorota Z. Haman, Ayse Kilic, James W. Jones, Kenneth L. Campbell, Thomas L. Crisman
Biological Systems Engineering: Papers and Publications
Two colors (white and black) of a recently introduced irrigation-plant production system [multi-pot box system (MPBS)] for container-grown nurseries were researched and results were compared with those obtained from the sprinkler-irrigated conventional (control) system (CS). Experiments were carried out in summer and fall of 2001 in Gainesville, Fla. Plant growth [growth index (GI), growth rate (GR), and dry matter] and stress parameters[stomatal resistance (rs), crop water stress index (CWSI), plant water potential (PWP), and substrate temperature (ST)] were measured and analyzed for Viburnum odoratissimum (Ker-gawl). In both seasons, plants grown in the white MPBS has significantly higher GI …
Bie6810 - Biochemical Engineering, Spring 2004, Ron Sims
Bie6810 - Biochemical Engineering, Spring 2004, Ron Sims
Biological and Irrigation Engineering - OCW
Biochemical processes, thermodynamics, and kinetics are used in the application of engineering principles to analyze, design, and develop processes using biocatalysts. Processes covered in the course include those that are involved in the formation of desirable compounds and products or in the transformation, or destruction of unwanted or toxic substances.
Leaching And Sorption Of Nitrogen And Phosphorus By Crop Residue, J. D. Cermak, John E. Gilley, Bahman Eghball, B. J. Weinhold
Leaching And Sorption Of Nitrogen And Phosphorus By Crop Residue, J. D. Cermak, John E. Gilley, Bahman Eghball, B. J. Weinhold
Biological Systems Engineering: Papers and Publications
Overland flow from cropland areas often contains nutrients and residue materials can either contribute to runoff nutrient load through leaching or remove nutrients by sorption. Measurements were made of leaching and sorption of nitrogen and phosphorus from corn (Zea mays L.), soybean (Glycine max (L.) Merr.) and winter wheat (Triticum aestivum L. cv. Pastiche) residues placed in solutions containing inorganic nutrients. Variables used were type of residue material, nutrient constituent, solution concentration, and residue / solution contact time. For a given residue material and nutrient constituent, four different solution concentrations were used (PO4 -P: 0 to …
Bie6150 - Surface Irrigation Design, Spring 2004, Wynn Walker
Bie6150 - Surface Irrigation Design, Spring 2004, Wynn Walker
Biological and Irrigation Engineering - OCW
This course was originally developed for the United States Department of Agriculture. It contains nearly 20 video lecture presentations with accompanying slides in English, Arabic, and French, an online textbook, homework assignments, and downloadable surface irrigation simulation software also developed by Dr. Walker. This course highlights design and evaluation of surface irrigation systems, field measurements for evaluating and improving uniformity and efficiency, simulation of surface systems, and land leveling computation and equipment. Note: The simulation software is designed to run on a PC only.
Controlled Release Systems For Dna Delivery, Angela K. Pannier, Lonnie D Shea
Controlled Release Systems For Dna Delivery, Angela K. Pannier, Lonnie D Shea
Biological Systems Engineering: Papers and Publications
Adapting controlled release technologies to the delivery of DNA has the potential to overcome extracellular barriers that limit gene therapy. Controlled release systems can enhance gene delivery and increase the extent and duration of transgene expression relative to more traditional delivery methods (e.g., injection). These systems typically deliver vectors locally, which can avoid distribution to distant tissues, decrease toxicity to nontarget cells, and reduce the immune response to the vector. Delivery vehicles for controlled release are fabricated from natural and synthetic polymers, which function either by releasing the vector into the local tissue environment or by maintaining the vector at …
Predicting Tractor Fuel Consumption, Robert D. Grisso, Michael F. Kocher, David H. Vaughan
Predicting Tractor Fuel Consumption, Robert D. Grisso, Michael F. Kocher, David H. Vaughan
Biological Systems Engineering: Papers and Publications
Reports from the Nebraska Tractor Test Laboratory (NTTL) show improved fuel efficiency during the past 20 years. A 4.8% decrease in average annual specific volumetric fuel consumption for the data used in the ASAE Standards was shown. Using fuel consumption and power data from the NTTL reports, new equations for fuel consumption were established that predict fuel consumption for diesel engines during full and partial loads and under conditions when engine speeds are reduced from full throttle.
Residual Effects Of Manure And Compost Applications On Corn Production And Soil Properties, Bahman Eghball, Daniel Ginting, John E. Gilley
Residual Effects Of Manure And Compost Applications On Corn Production And Soil Properties, Bahman Eghball, Daniel Ginting, John E. Gilley
Biological Systems Engineering: Papers and Publications
Residual effects of manure or compost application on crop production and soil properties can last for several years. This study was conducted to evaluate residual effects of annual or biennial applications of N- and P-based composted and non-composted beef cattle (Bos Taurus) feedlot manure, chemical fertilizer, and no-treatment check on corn (Zea mays L.) production and soil properties. Manure and compost were applied from 1992 to 1995, and the residual effects were determined from 1997 to 1999. Residual effects of N- and P-based manure and compost applications on corn grain yield and N uptake lasted for at …
Policosanol Contents And Composition Of Grain Sorghum Kernels And Dried Distillers Grains, Keum T. Hwang, Curtis L. Weller, Susan L. Cuppett, Milford Hanna
Policosanol Contents And Composition Of Grain Sorghum Kernels And Dried Distillers Grains, Keum T. Hwang, Curtis L. Weller, Susan L. Cuppett, Milford Hanna
Biological Systems Engineering: Papers and Publications
Grain sorghum can be a major source of policosanols, long-chained alcohols, that have beneficial physiological activities. Sorghum dried distillers grains (DDG), a by-product of ethanol production from grain sorghum. contain a large amount of policosanols. Content and composition of policosanols in long-chained lipids extracted from grain sorghum kernels and DDG were determined. Long-chained lipids were extracted using hot hexane or hot ethanol. The major components of the long-chained lipids extracted from grain sorghum kernels. as determined using HPLC were policosanols (37-44%), aldehydes (44-55%), and acids (4-5%). Long-chained lipids from DDG contained 52% policosanols, 23% aldehydes. 6.4% acids. and l7% wax …
Field Efficiency Determination Using Traffic Pattern Indices, Robert D. Grisso, Michael F. Kocher, Viacheslav I. Adamchuk, Paul J. Jasa, Mark A. Schroeder
Field Efficiency Determination Using Traffic Pattern Indices, Robert D. Grisso, Michael F. Kocher, Viacheslav I. Adamchuk, Paul J. Jasa, Mark A. Schroeder
Biological Systems Engineering: Papers and Publications
Field efficiency is an important criterion for determining field capacity during field operations and, indirectly, for making important machinery management decisions. Geographic location data gathered with a yield monitor during harvest and a data logger during planting were used to provide time−motion studies of equipment and operator productivity. This study used these spatial and temporal data to quantify field performance of a combine and a planter. Seven Nebraska fields were used to compare results from soybean and corn production systems. Fields that were relatively flat with straight rows were contrasted with contoured fields with slopes of 3% to 5%. Two …
Improving Heating Uniformity Of Fresh Fruit In Radio Frequency Treatments For Pest Control, Sohan Birla, Shaojin Wang, Juming Tang, Guy Hallman
Improving Heating Uniformity Of Fresh Fruit In Radio Frequency Treatments For Pest Control, Sohan Birla, Shaojin Wang, Juming Tang, Guy Hallman
Biological Systems Engineering: Papers and Publications
Non-uniform heating of fresh fruit caused by variations in radio frequency (RF) fields is a major obstacle in developing postharvest insect control treatments based on RF energy. A fruit mover was developed to evaluate possibilities to improve RF heating uniformity of large fruit, such as oranges and apples, in a 12 kW batch type RF system. This fruit mover provided a means to rotate and move fruit in water when subjected to RF heating. Parameters for moving and rotating fruit in the mover were selected based on consideration of vortex formation, foaming, damage to the fruit, and volume occupied by …