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Articles 1 - 8 of 8
Full-Text Articles in Biological Engineering
Hypoxic Incubator: Improving Robustness/Reliability And Demonstrating Physiological Efficacy, Damon Dennis Tan
Hypoxic Incubator: Improving Robustness/Reliability And Demonstrating Physiological Efficacy, Damon Dennis Tan
Master's Theses
The Microphysiological Systems Laboratory aims to develop colorectal cancer tumor models under a hypoxic environment to assess model response to pharmaceutical compounds in vitro. To perform relevant studies, researchers have attempted to use different hypoxic inducing strategies such as a nitrogen pod and hypoxic incubator to recreate in vivo physiological responses to hypoxia. However, studies would be interrupted due to incubator functionality failure. To ensure successful and physiologically relevant studies, I improved and verified the robustness and reliability of a hypoxic incubator previously designed and manufactured in the lab. Through the testing and iterating design processes, I engineered and implemented …
Development Of An Angiogenic Tissue-On-A-Chip Microenvironment, Eric Stuehr
Development Of An Angiogenic Tissue-On-A-Chip Microenvironment, Eric Stuehr
Master's Theses
Preclinical testing is necessary to investigate the safety and efficacy of novel therapeutics before moving to clinical trials, yet approximately 90% of these therapies fail once tested in humans. This has led to increased interest in developing robust preclinical models that accurately mimic the complex human in vivo physiology. Microfluidic devices that can introduce dynamic conditions to 3D cell/organoid cultures, also known as tissue-on-a-chip, have emerged as physiologically relevant in vitro preclinical models that can achieve high throughput screening of therapeutics. The research presented here aimed to develop an angiogenic environment within a novel microfluidic device to stimulate formation of …
Effect Of Hypoxia On Spontaneous Neural Activity In The Cortex Of Neonate Mouse Pups, Krithikka Ravi Ms
Effect Of Hypoxia On Spontaneous Neural Activity In The Cortex Of Neonate Mouse Pups, Krithikka Ravi Ms
Dissertations and Theses
Hypoxia caused by inadequate oxygenation has profound effects on the normal functioning of the brain in mammals. Acute or chronic hypoxic insults occur in the brain depending on the duration of hypoxic exposure. Hypoxia is known to occur in the human womb and exerts adverse effects on the developing fetus. Most of the ongoing research on hypoxia is performed on rodent brain slice taken from various brain regions using intracellular recording. Extensive work has been carried out to understand the effects of chronic hypoxia on the developing nervous system, specifically during intrauterine development. However, effects of acute hypoxia occurring perinatally, …
Developing Droplet Based 3d Cell Culture Methods To Enable Investigations Of The Chemical Tumor Microenvironment, Jacqueline Alexandria De Lora
Developing Droplet Based 3d Cell Culture Methods To Enable Investigations Of The Chemical Tumor Microenvironment, Jacqueline Alexandria De Lora
Biomedical Sciences ETDs
Adaptation of cancer cells to changes in the biochemical microenvironment in an expanding tumor mass is a crucial aspect of malignant progression, tumor metabolism, and drug efficacy. In vitro, it is challenging to mimic the evolution of biochemical gradients and the cellular heterogeneity that characterizes cancer tissues found in vivo. It is well accepted that more realistic and controllable in vitro 3D model systems are required to improve the overall cancer research paradigm and thus improve on the translation of results, but multidisciplinary approaches are needed for these advances. This work develops such approaches and demonstrates that new droplet-based cell-encapsulation …
Fundamental Characterization Of Oxygen Nanobubbles, John Hamlin, Yi Wen, Joseph Irudayaraj
Fundamental Characterization Of Oxygen Nanobubbles, John Hamlin, Yi Wen, Joseph Irudayaraj
The Summer Undergraduate Research Fellowship (SURF) Symposium
A hypoxic environment is created by tumors’ incredible growth rate. Hypoxia provides radioresistance to the tumors, thus making radiation treatment less effective. The issue is that increasing the radiation leads to increased side effects in patients. Our goal for the oxygen-filled nanobubble is to deliver oxygen to the tumor to lessen radioresistance and make radiation treatment more efficient. However, we need preliminary research to understand and improve the nanobubbles before further research and implementation. To do this, we synthesized different batches of nanobubbles to optimize the production method and find the best container and temperature to store nanobubbles. We measured …
Nanobubbles Provide Theranostic Relief To Cancer Hypoxia, Christopher M. Long, Pushpak N. Bhandari, Joseph Irudayaraj
Nanobubbles Provide Theranostic Relief To Cancer Hypoxia, Christopher M. Long, Pushpak N. Bhandari, Joseph Irudayaraj
The Summer Undergraduate Research Fellowship (SURF) Symposium
Hypoxia is a common motif among tumors, contributing to metastasis, angiogenesis, cellular epigenetic abnormality, and resistance to cancer therapy. Hypoxia also plays a pivotal role in oncological studies, where it can be used as a principal target for new anti-cancer therapeutic methods. Oxygen nanobubbles were designed in an effort to target the hypoxic tumor regions, thus interrupting the hypoxia-inducible factor-1α (HIF-1α) regulatory pathway and inhibiting tumor progression. At less than 100nm, oxygen nanobubbles act as a vehicle for site-specific oxygen delivery, while also serving as an ultrasound contrast agent for advanced imaging purposes. Through in vitro and in vivo studies, …
Long-Term Manure And Fertilizer Application Effects On Phosphorus And Nitrogen In Runoff, Bahman Eghball, John E. Gilley, David D. Baltensperger, J. M. Blumenthal
Long-Term Manure And Fertilizer Application Effects On Phosphorus And Nitrogen In Runoff, Bahman Eghball, John E. Gilley, David D. Baltensperger, J. M. Blumenthal
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Long–term manure and fertilizer applications to a soil can increase phosphorus (P) and nitrogen (N) transport in runoff. This study was conducted to determine P and N transport in runoff following long–term (since 1953) manure and fertilizer applications. Duplicate soil samples (32) were collected in 1998 from the top 0.1 m of selected plots of a long–term manure and fertilizer applications field experiment and later placed in 1 m2 soil pans in the laboratory. Manure and fertilizer were mixed with 16 of the soil samples, while no treatment was applied to the other half (long–term residual effect). Simulated rainfall …
Narrow Grass Hedge Effects On Phosphorus And Nitrogen In Runoff Following Manure And Fertilizer Application, B. Eghball, John E. Gilley, L. A. Kramer, T. B. Moorman
Narrow Grass Hedge Effects On Phosphorus And Nitrogen In Runoff Following Manure And Fertilizer Application, B. Eghball, John E. Gilley, L. A. Kramer, T. B. Moorman
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Runoff losses of nitrogen (N) and phosphorus (P) from field applied manure can contribute to surface water pollution. Grass hedges may reduce runoff losses of nutrients and sediment. The objective of this study was to evaluate the effects of narrow switchgrass (,i>Panicum virgatum L.) hedges (~0.75 m wide) on the transport of P and N from a field receiving beef cattle feedlot manure under tilled and n-till conditions. This study was conducted on a steep (12% average slope) Monona silt loam (fine-silty, mixed, superactive, mesic Typic Hapludolls) soil near Treynor, Iowa. The experiment was a split-plot with no-till and …