Biological Engineering Commons^™

Uv-A Light: A Novel Method For Food Dehydration, Mckade S. Roberts

All Graduate Theses and Dissertations, Fall 2023 to Present

Dehydration is one of the most prevalent forms of food preservation, with approximately 85% of food is hot air drying due to the simplicity of the process. The high temperatures employed in this method may also induce a loss in nutritional quality, which involves destruction of micronutrients (vitamins), and loss of color.

The aim of this work is to develop a low energy, non-thermal method of food dehydration that results in a high-quality product through the application of Ultraviolet light, within the UV-A range. UV light can be separated into its 3 categories based on the wavelength of light from …

Adjuvant Pluronic F68 Is Compatible With A Plant Root-Colonizing Probiotic, Pseudomonas Chlororaphis O6, Amanda R. Streeter, Anthony Cartwright, Mohammad Zargaran, Anagha Wankhade, Anne J. Anderson, David W. Britt

Biological Engineering Faculty Publications

Plant probiotic bacteria are being increasingly used to maximize both the productivity and quality of field crops. Pseudomonas chlororaphis O6 (PcO6) is a plant root colonizer with probiotic activities. This bacterium produces an array of metabolites, including a group of phenazines that are functional in plant protection. The paper reports responses of PcO6 to a nonionic triblock copolymer surfactant, Pluronic F68. This Pluronic exhibits membrane "healing" activity and improves cryopreservation recovery in eukaryotic cells. The product is FDA-approved and is applied as an adjuvant in formulations used in agriculture, medicine, and biotechnology. Growth of PcO6 on …

Effects Of Indole Derivative Idc16 On Cell Proliferation, Transfection, And Alternative Splicing In Production Of Lentiviral Vectors By Transient Co-Transfection, Nataly Mier

All Graduate Theses and Dissertations, Fall 2023 to Present

Viruses can efficiently introduce genetic material into a variety of cells through cell infection. Therefore, viral vectors, defined as virus-based gene delivery systems, effectively transfer functional gene constructs to target cells. Among different viral vectors, lentiviral vectors are of interest due to the advantages offered to research and therapeutic applications. Low productivity of lentiviral vectors in mammalian producer cells and high production cost, however, remain barriers to the vectors’ routinary use, particularly in clinical settings.

Since most lentiviral vectors are derived from human immunodeficiency virus type I, their genome has been engineered to increase safety. Nevertheless, modifications of the lentiviral …

Microbial Glycosylation Of Antitubercular Agent Chlorflavonin, Jie Ren, Jixun Zhan

Biological Engineering Faculty Publications

Flavonoids have shown health-benefiting properties, such as antioxidative and anti-inflammatory activities, and are commonly used as nutraceuticals and pharmaceuticals. Although flavonoids are predominantly identified from plants, several filamentous fungal species have also been reported to produce bioactive flavonoids, including chlorflavonin from Aspergillus candidus, a novel halogenated flavonoid with potent antifungal and antitubercular (anti-TB) activities. Unfortunately, the low water-solubility of this molecule may hinder its bioavailability. Glycosylation is an effective method to enhance the polarity of natural products and alter their physicochemical properties. This work focuses on the development of novel water-soluble chlorflavonin derivatives to combat the threat of drug-resistant …

Exploring Versatile Glycosyltransferases From Microorganisms For Natural Product Biosynthesis, Jie Ren

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This dissertation is mainly focused on exploring versatile glycosyltransferases from different microorganisms with the purpose of biosynthesizing bioactive natural product glycosides through microbial fermentation. This engineered biosynthesis approach is environmentally friendly, safe, and efficient compared with plant extraction, chemical synthesis, and enzymatic synthesis. Additionally, we applied these characterized glycosyltransferases as efficient biocatalytic tools to produce novel glycosides to expand the chemical repertoire of nature. These novel compounds showed improved water solubility and some of them exhibited enhanced stability and biological activity, which laid the foundation for the development of new therapeutic agents.

In my dissertation research, I have discovered two …

Environmentally Friendly New Catalyst Using Waste Alkaline Solution From Aluminum Production For The Synthesis Of Biodiesel In Aqueous Medium, Sandro L. Barbosa, David Lee Nelson, Lucas Paconio, Moises Pedro, Wallans Torres Pio Dos Santos, Alexandre P. Wentx, Fernando L. P. Pessoa, Foster A. Agblevor, Daniel A. Bortoleto, Maria B. De Freitas-Marques, Lucas D. Zanatta

Biological Engineering Faculty Publications

Red mud (RM) is composed of a waste alkaline solution (pH = 13.3) obtained from the production of alumina. It contains high concentrations of hematite (Fe₂O₃), goethite (FeOOH), gibbsite [Al(OH)₃], a boehmite (AlOOH), anatase (Tetragonal–TiO₂), rutile (Ditetragonal dipyramidal–TiO₂), hydrogarnets [Ca₃Al₂(SiO₄)_3-x(OH)_4x], quartz (SiO₂), and perovskite (CaTiO₃). It was shown to be an excellent catalytic mixture for biodiesel production. To demonstrate the value of RM, an environmentally friendly process of transesterification in aqueous medium using waste cooking oil …

An Integrated Experimental And Modeling Approach To Design Rotating Algae Biofilm Reactors (Rabrs) Via Optimizing Algae Biofilm Productivity, Nutrient Recovery, And Energy Efficiency, Gerald Benjamin Jones

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Microalgae biofilms have been demonstrated to recover nutrients from wastewater and serve as biomass feedstock for bioproducts. However, there is a need to develop a platform to quantitatively describe microalgae biofilm production, which can provide guidance and insights for improving biomass areal productivity and nutrient uptake efficiency. This paper proposes a unified experimental and theoretical framework to investigate algae biofilm growth on a rotating algae biofilm reactor (RABR). The experimental laboratory setups are used to conduct controlled experiments on testing environmental and operational factors for RABRs. We propose a differential-integral equation-based mathematical model for microalgae biofilm cultivation guided by laboratory …

Engineered Production Of Bioactive Polyphenolic O-Glycosides, Jie Ren, Caleb Don Barton, Jixun Zhan

Biological Engineering Faculty Publications

Polyphenolic compounds (such as quercetin and resveratrol) possess potential medicinal values due to their various bioactivities, but poor water solubility hinders their health benefits to humankind. Glycosylation is a well-known post-modification method to biosynthesize natural product glycosides with improved hydrophilicity. Glycosylation has profound effects on decreasing toxicity, increasing bioavailability and stability, together with changing bioactivity of polyphenolic compounds. Therefore, polyphenolic glycosides can be used as food additives, therapeutics, and nutraceuticals. Engineered biosynthesis provides an environmentally friendly and cost-effective approach to generate polyphenolic glycosides through the use of various glycosyltransferases (GTs) and sugar biosynthetic enzymes. GTs transfer the sugar moieties from …

Increasing Production Of Therapeutic Mabs In Cho Cells Through Genetic Engineering, Charles Barentine

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Between 2014 and 2018, the global market for therapeutic monoclonal antibodies (mAbs) rose from $60 billion to $115.2 billion with a projected value of $300 billion by 2025. These molecules are used to effectively treat some of the most challenging illnesses from auto-immune diseases to cancer. While mAbs are highly valuable with potent applications, their production at scale remains an outstanding challenge. These molecules are largely produced in Chinese Hamster Ovary (CHO) cells that require highly specific conditions to produce a useful product.

Genetic engineering presents one solution to overcome productivity limits. With the advent of CRISPR (clustered regularly interspaced …

Low Resource Assay For Tracking Sars-Cov-2 In Wastewater, Julissa Van Renselaar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wastewater based epidemiology (WBE) allows for the tracking of nucleic acid of SARS-CoV-2 in wastewater which gives the opportunity for the public and government officials to be informed about the infectivity of the virus in a community. Advances have been made in WBE that have allowed for higher performance, lower resource use, and faster turnaround time. An adapted concentration method of spin column direct extraction has yielded a proxy virus recovery of 83%, consumable cost of $2.01 per sample, and a turnaround time of 0.33 hour/sample. Other concentration methods have lower proxy virus recoveries, comparable cost, and comparable turnaround time. …

Upstream Methods For Enhancing Engineered Curcumin Biosynthesis, Caleb D. Barton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Curcumin is a bright orange compound with myriad applications for human health and wellness. Curcumin occurs naturally in the plant Curcuma longa (commonly known as turmeric) but must be extracted from the roots in an environmentally unfriendly fashion to obtain commercially relevant amounts of the compound. In addition, extraction of curcumin from turmeric spice yields a mixture of various curcuminoids, presenting an issue for isolating it in its pure form and complicating its use in clinical settings.

Heterologous biosynthetic production of curcumin in Escherichia coli has been used extensively as a viable alternative to plant extraction but suffers from poor …

What Drug Candidates Exist For Treating Covid-19 Variants?, Kolton Hauck

Research on Capitol Hill

USU senior Kolton studies biological engineering and led this project with funding from a student grant. Kolton’s project has been mapping out all possible protein interactions from known SARS-CoV-2 variants and identifying possible drug treatments. Viruses function through these interactions between their proteins and the host cell’s proteins, and this project is especially valuable because the candidates identified are predicted to help treat all known variants, making them more likely to be effective against future mutations.Kolton’s interest in biological research stems from his own experience managing a genetic health disorder. Past medical research greatly benefited him, and he sees his …

Utilizing Nasa-Funded Biotechnology To Improve Resource Management On Earth And In Space, Tyler Wallentine

Research on Capitol Hill

USU senior Tyler is a Peak Summer Research Fellow studying biological engineering. Nitrogen, essential in soil fertilizer for crops, is produced traditionally in a way that uses natural gas and produces CO2. Tyler’s project has been to apply methods developed by NASA for astronauts to conserve and reuse resources to create nitrogen using wastewater and bacteria. Using this method would not only take advantage of waste we already have, but doesn’t produce CO2 and contribute to pollution. Tyler hopes to become a chemical engineer in the space industry and credits his undergraduate research experience. “There’s something about having to obtain …

Can Cannibinoids Be Used To Prevent Lung Damage From Utah Air Pollution?, Emily Brothersen

Research on Capitol Hill

USU senior Emily, a Mantua native, is an Honors student and Undergraduate Research Fellow. She studies Biological Engineering and has funded and led this project herself with multiple student grants. Emily has been investigating a way to study cell health that can help future researchers test treatments more quickly and affordably. She has used this method to research the potential of cannabinoids to help protect people from lung damage caused by pollution, an issue topical to Utah with our frequent inversions and growing medical cannabis industry. Emily has been involved in research throughout her time at USU and sees it …

Assessing Leachable Cytotoxicity Of 3d-Printed Polymers And Facile Detoxification Methods, Venkatakrishnan Rengarajan, Angela Clyde, Jefferson Pontsler, Jonathan Valiente, Adreann Peel, Yu Huang

Biological Engineering Faculty Publications

Additive manufacturing of polymers is gaining momentum in health care industries by providing rapid 3D printing of customizable designs. Yet, little is explored about the cytotoxicity of leachable toxins that the 3D printing process introduced into the final product. We studied three printable materials, which have various mechanical properties and are widely used in stereolithography 3D printing. We evaluated the cytotoxicity of these materials through exposing two fibroblast cell lines (human and mouse derived) to the 3D-printed parts, using overlay indirect contact assays. All the 3D-printed parts were measured toxic to the cells in a leachable manner, with flexible materials …

Effectiveness Of Cannabidiol And Resveratrol Against Diesel Exhaust Particle-Induced Lung Cell Cytotoxicity, Emily Brothersen

Fall Student Research Symposium 2021

Environmental air pollution poses a significant health risk to individuals across the world. Diesel exhaust particles (DEPs), a major component of air pollution, have been shown to cause lung damage leading to cancer, respiratory infections, and premature death. Antioxidants, such as resveratrol, have previously demonstrated protective properties against DEP-induced cytotoxicity and reactive oxygen species. Cannabinoids extracted from hemp species have also been found to have powerful antioxidant properties, though these properties have not been thoroughly explored. In this study, A549 human lung carcinoma cells were used as a cellular model to determine the effectiveness of cannabinoids’ antioxidant properties against DEP-induced …

Reu Site: Stem For Plant Health, David W. Britt

Funded Research Records

No abstract provided.

Tuning Radiation And Microgravity Exposure For Human Brain Organoids, Bailey Mcfarland

Utah Space Grant Consortium

The environment of space, specifically radiation and microgravity, poses a substantial threat to astronauts' brain health. Understanding the damage mechanism and therefore paving the way to finding potential remedies is critical to protect astronauts during space flight. Current models are insufficient at representing the human brain, and insufficient research has been conducted on the effect of radiation and microgravity on the higher structures of the human brain. This ongoing research aims to use brain organoids, a tissue-engineered model that recapitulates the human brain's anatomy and structure and investigate the effects of radiation and microgravity on the brain. At the current …

Biomethanation And Alkaline Wet Air Oxidation Of Water Hyacinth (Pontederia Crassipes) From Ozama River, Dominican Republic, Yessica A. Castro

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Obtaining valuable products from environmental remediation waste is a sustainable approach that contributes to the ecological well-being of developing countries. In the present work, the feasibility of the water hyacinth anaerobic digestion as a post-weed management practice in the Ozama River (Dominican Republic) was demonstrated. The estimated energy required for harvesting was lower than that produced during digestion. The biomethanation of water hyacinth was improved by Alkaline Wet Air Oxidation (AWAO), a thermochemical pretreatment process that almost doubled the methane production rate and increased the yield by 24% when conducted at high temperatures. At lower temperatures, the methane yield of …

Deciphering And Engineering An Unprecedented Fungal Biosynthetic Pathway For Expanded Chemical Diversity In Flavonoids, Jixun Zhan

Funded Research Records

No abstract provided.

Functional Complementation Of The Ppgcn4 And Ppnhx2 Genes In Arabidopsis Thaliana To Study Salt Tolerance, Amanda Moravek

Fall Student Research Symposium 2020

Climate change induces unexpected weather and causes abiotic and biotic stresses in plants. These stresses negatively affect crop growth and production. Additionally, a steady increase in the world population has been leading to higher food demand. Therefore, the development of more stress-resilient crops is essential to combat these problems. One such stress is a salinity that affects crop production. Almond is a salt-sensitive crop, so there is a need to identify salt-tolerant almond rootstocks. AtGCN4 is a novel gene that was identified in Arabidopsis thaliana to play a significant role in host-pathogen interaction and drought tolerance when overexpressed. The preliminary …

Dogs In Ancient Egypt, Ella Olson

Fall Student Research Symposium 2020

The Ancient Egyptians are infamous for their love of cats, but what about dogs? It turns out that dogs were equally loved and praised in Egyptian culture. A large part of this comes from the religious significance of dogs- they are connected to the afterlife through Anubis, the god of the dead, and were thought to act as companions and guides to humans in the afterlife. Anubis is an interesting god to look at because he's one of the oldest and most respected gods. In today's terms, we refer to him as having a jackal head, but the Egyptians considered …

Cytomegalovirus Inhibition By Pluronic-Encapsulated Quercetin And Synergy With Ganciclovir, Ian Wadsworth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Congenital cytomegalovirus (CMV) is the leading viral cause of birth defects worldwide and leads to a variety of complications. Ganciclovir is the most common anti-CMV drug used but often causes severe side effects. Quercetin, a plant pigment, shows anti-CMV activity that may provide a less toxic alternative to ganciclovir, or when co-administered, reduce ganciclovir treatments. However, a drug carrier is needed to deliver quercetin for CMV treatment. In this study, three Pluronic polymers (P123, F127, and F68) were investigated as a quercetin drug delivery system (DDS) for CMV treatment.

All selected Pluronics successfully encapsulated quercetin at varying degrees of efficacy. …

Characterization Of Chinese Hamster Ovary Cell Performance With Pentanoic Acid And N-Acetyl Cysteine Treatment, Joseph Camire

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To improve the cost of producing protein therapeutics manufacturers can attempt to increase the amount of protein produced per unit volume. Some small fatty-acids have shown the ability to increase protein concentrations in some manufacturing systems. An example system is in the industrially important cell line derived from a Chinese Hamster Ovary (CHO). A drawback of adding these fatty-acids is they can increase the rate of cell death.

A potential way to minimize the cell death rate, caused by adding fatty-acids, is to also add additional anti-oxidants. Through a series of studies, we demonstrate production with different fatty-acids, butanoic acid …

Protecting Crops From Abiotic Stress: Copper Oxide Nanoparticle Effects On Wheat And A Beneficial Rhizobacterium, Matthew Potter

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nanoparticles (NPs) are defined as particles less than 100 nm in at least one dimension. CuO NPs have possible applications in agriculture as micronutrient sources, pesticides, and enhancers of crop stress tolerance. Here, three aspects of CuO NP agricultural applications are studied: 1) the potential of CuO NPs to prevent wheat lodging-when crops irreversibly fall over; 2) CuO NP-induced drought tolerance in wheat seedlings; and 3) the effects of CuO NPs on outer membrane vesicle (OMV) production by Pseudomonas chlororaphis O6 (PcO6), a plant-health promoting bacterium.

Wheat grown 7 d exposed to CuO NPs in the growth matrix …

Discovery Of A Novel Analogue Of Fr901533 And The Corresponding Biosynthetic Gene Cluster From Streptosporangium Roseum No. 79089, Fuchao Xu, Yonghong Liang, Jie Ren, Siyuan Wang, Jixun Zhan

Biological Engineering Faculty Publications

FR901533 (1, also known as WS79089B), WS79089A (2), and WS79089C (3) are polycyclic aromatic natural products with promising inhibitory activity to endothelin-converting enzymes. In this work, we isolated five tridecaketide products from Streptosporangium roseum No. 79089, including 1-3, benaphthamycin (4) and a novel FR901533 analogue (5). The structure of 5 was characterized based on spectroscopic data. Compared to the major product 2, the new compound 5 has an additional hydroxyl group at C-12 and an extra methyl group at the 13-OH. The configuration of C-19 of these compounds …

Existing Empirical Kinetic Models In Biochemical Methane Potential (Bmp) Testing, Their Selection And Numerical Solution, Yehor Pererva, Charles D. Miller, Ronald C. Sims

Biological Engineering Faculty Publications

Biochemical Methane Potential (BMP) tests are a crucial part of feasibility studies to estimate energy recovery opportunities from organic wastes and wastewater. Despite the large number of publications dedicated to BMP testing and numerous attempts to standardize procedures, there is no “one size fits all” mathematical model to describe biomethane formation kinetic precisely. Importantly, the kinetics models are utilized for treatability estimation and modeling processes for the purpose of scale-up. A numerical computation approach is a widely used method to determine model coefficients, as a replacement for the previously used linearization approach. However, it requires more information for each model …

Isolation And Selective Glycosylation Of Antisalmonellal Anthraquinones From The Stem Bark Of Morinda Lucida Benth. (Rubiaceae), Napoleon A. Mfonku, James A. Mbah, Norbert Kodjio, Donatien Gatsing, Jixun Zhan

Biological Engineering Faculty Publications

In this work we report the isolation, identification and antibacterial activity of two anthraquinones, 2-hydroxy-1-methoxyanthraquinone (1) and 2,5-dihydroxy-1-methoxy-6-methoxymethylanthraquinone (2), from the stem bark of Morinda lucida. These two natural products were selectively converted into two new glycosylated derivatives, 2-hydroxy-1-methoxyanthraquinone-4′-O-methyl-2-O-β-d-glucopyranoside (3) and 2,5-dihydroxy-1-methoxy-6-methoxymethylanthraquinone-4′-O-methyl-2-O-β-d-glucopyranoside (4) by the filamentous fungus Beauveria bassiana ATCC 7159. Structure elucidation was accomplished based on the 1D and 2D NMR, IR and mass spectra. The glycosylated compounds 3 and 4 showed higher in vitro antibacterial activity against Salmonella enterica subsp. enterica sérovars Typhimurim (MIC of 8 μg/mL each) than the corresponding aglycons 1 and 2 (MIC of …

Determination Of Gas Emission Characteristics From Animal Wastes Using A Multiplexed Portable Ftir-Surface Chamber System, Pakorn Sutitarnnontr

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Livestock production is a growing source of air pollution at regional, national, and global scales. Improved livestock manure management has the potential to reduce environmental impacts; however, there is an urgent need for cost-efficient, reliable, and easy to maintain measurement and monitoring capabilities to precisely quantify emissions from livestock manure. This research describes and evaluates a novel measurement method based on the multiplexed portable Fourier Transform Infrared (FTIR) spectroscopy analyzer - surface chamber techniques for continuous measurements and monitoring gas emissions from manure sources. The multiplexing system was designed and developed to automate the chamber network, controlling the movement of …

Observation Of Struvite In The Mixed Microalgae Biofilm Matrix Of A Rotating Algal Biofilm Reactor During Nutrient Removal From Municipal Anaerobic Digester Filtrate, Kyle M. Hillman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Central Valley Water Reclamation Facility (CVWRF) in Salt Lake City is the largest municipal wastewater treatment plant in Utah and must meet new and rigorous nutrient effluent standards–over 95% reduction in phosphorus output by 2025. Filtrate from CVWRF anaerobic digesters contains high levels of nitrogen, phosphorus, and magnesium. Supersaturation of these constituents leads to nuisance struvite precipitation that clogs belts, pumps, and pipes downstream of anaerobic digesters. Struvite is a mineral precipitate composed of equimolar magnesium, ammonium, and phosphate. Controlled precipitation of struvite helps prevent clogging and scaling, removes phosphate from wastewater, and generates a marketable fertilizer product.

Struvite was …