Chemical Engineering Commons^™

Dendrimer Gel Nanoparticles With Dual-Targeting Of Upar And Ribonucleotide Reductase R2 Influence Necrosis, Apoptosis, And Cell Cycle Arrest In Triple-Negative Breast Cancer, Hsin Yin Chuang, Yue-Wern Huang, Hu Yang

Biological Sciences Faculty Research & Creative Works

Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited treatment options due to the absence of estrogen (ER), progesterone (PR), and human epidermal growth factor receptor 2 (HER2) receptors. We previously developed highly adaptable polyamidoamine (PAMAM) dendrimer-based gel nanoparticles (GDP-uPA/GTI) with dual-targeting capabilities against urokinase-type plasminogen activator receptor (uPAR) and ribonucleotide reductase R2 (R2) to achieve selective antitumor effects. In this study, we investigated the underlying mechanisms of GDP-uPA/GTI-induced tumor inhibition. In vivo, GDP-uPA/GTI significantly downregulated R2 expression and activated caspase 8 and caspase 3, indicating caspase-dependent apoptosis. Histological evaluation of major organs revealed no observable toxicity. …

Bone Void Filler Materials For Augmentation In Comminuted Fractures: A Comprehensive Review, S. Sina Mohammadi, Sunjeev S. Phull, B. Sonny Bal, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

Comminuted fractures, characterized by multiple bone fragments, present significant challenges in orthopedic surgery. Effective treatment often requires augmentation techniques to enhance fixation stability and promote bone regeneration. This review explores the application of bone void filler materials, including autografts, allografts, polymethyl methacrylate (PMMA), and synthetic bone substitutes such as calcium phosphate ceramics and bioactive glass, in managing comminuted fractures. Autografts are the gold standard due to their superior osteogenic potential but are limited by donor site morbidity and availability. Allografts mitigate these issues but face concerns regarding immunogenicity and reduced biological activity. PMMA, widely used for structural augmentation, provides immediate …

Theory And Technology Of Enhanced Coal Seam Gas Production By Integrated Drilling, Hydraulic Punching And Fracturing In Coal Mine: Part 2 Technology, Shiyao Yu, Xianbo Su, Jinxing Song, Qian Wang, Zhenjiang You

Research outputs 2022 to 2026

To enhance the efficiency and cost-effectiveness of coal seam gas extraction in coal mines, this study introduces an integrated approach combining drilling, hydraulic punching, and fracturing technologies. This method optimizes drilling media tailored to specific coal seams and fine-tunes technical parameters during drilling and permeability improvement processes. Furthermore, it clarifies the relationships among the coal breaking rate, discharge of drill cuttings, jet velocity, rotation speed, and displacement. This ensures precise and effective permeability improvement in both soft and hard coal formations. To implement this technology, a set of high-performance drilling tool with a large inner diameter, high pressure resistance, and …

The Significant Impact Of Hot Isostatic Pressing On The Corrosion Behavior Of Ti-6al-4v Alloy Prepared By Electron Beam Powder Bed Fusion, Hongyu Zheng, Yun Bai, Wentao Hou, Zirong Zhai, Lai Chang Zhang, Shujun Li, Rui Yang

Research outputs 2022 to 2026

This work investigated the effect of hot isostatic pressing (HIP) on the corrosion behavior of Ti-6Al-4V samples prepared by electron beam powder bed fusion (EB-PBF). After the HIP treatment, the TiO2 content decreased but the growth rate of passivation films increased, leading to increase in internal defect density, thereby degrading the corrosion resistance. Amongst the different factors impacted by HIP including pore defects, β-phase, texture and morphology of α-phase, the morphology of α-phase played a dominant role in determining the corrosion performance of EB-PBF processed alloy.

Enhanced Degradation Of Triclosan Using Aerated Hydrodynamic Cavitation: Turbulence-Based Modelling And Economic Evaluation, Mehdi Khiadani, Ensiyeh Taheri, Veena Bobade, Ali Fatehizadeh

Research outputs 2022 to 2026

Pharmaceutical and personal care product (PPCP)-contaminated wastewater, particularly with triclosan (TCS), poses a significant environmental concern due to TCS persistence, ecotoxicity, and endocrine-disrupting effects, necessitating the development of efficient and sustainable remediation technologies. In this study, the degradation of TCS using hydrodynamic cavitation coupled with air injection (aerated HC process) is investigated as a green and chemical-free treatment method for wastewater treatment. Initially, the optimum operating conditions of the standalone HC process were determined by varying the inlet pressure and flow rate, which were found to be 2.34 bar and 0.45 L/s, respectively, corresponding to the highest turbulence frequency level …

Experimental Study On Stability, Rheology, And Co2 Storage Capacity Of Polymer-Enhanced Foams In Carbonates, Muhammad Mushtaq, Mudassar Mumtaz, Emad W. Al-Shalabi, Waleed Alameri, Georgios N. Karanikolos, Stefan Iglauer

Research outputs 2022 to 2026

Polymer-enhanced CO2 foam (PEF) is an emerging technology that improves gas mobility control in chemical enhanced oil recovery applications. It also has significant potential for storing CO2 in geological formations, which can help mitigate anthropogenic emissions. Traditional surfactant-based foams are often weak and unstable, particularly under the high-salinity and high-temperature conditions commonly found in Middle Eastern carbonate reservoirs. However, adding a small amount of stable polymer to these foams can enhance their stability, rendering them more suitable for harsh environments. This study focuses on designing and thoroughly evaluating a PEF formulation under supercritical CO2 conditions at 95 °C temperature, 1500 …

Interface Engineering And Safety In Solid-State Batteries: Advancing From Human-Centered Insights To Ai-Driven Innovations, Elnaz Karimi, Stefan Iglauer, Muhammad Rizwan Azhar

Research outputs 2022 to 2026

Solid-state batteries (SSBs) represent a transformative advancement in energy storage, offering superior safety, higher energy density and extended cycle life compared to conventional lithium-ion batteries (LIBs). However, challenges related to interface engineering—particularly in ensuring stable electrochemical performance and preventing lithium dendrite formation—have hindered their widespread adoption and can compromise safety. Effective interface engineering is critical for mitigating interfacial resistance, enhancing mechanical stability and preventing thermal runaway, all of which are vital for improving battery reliability. The integration of artificial intelligence (AI) and machine learning (ML) in this context accelerates battery optimization by enabling predictive modelling of interfacial behaviour, material discovery …

Performance Study Of A Woody Downdraft Gasifier: Numerical Investigation And Experimental Validation, Md Sanowar Hossain, Showmitro Bhowmik, Mujahidul Islam Riad, Md Golam Kibria, Barun K. Das, Sanjay Paul

Research outputs 2022 to 2026

Biomass gasification is an established and widely utilized renewable energy system. The research work aims to develop and construct a downdraft gasifier to investigate gasifier performance. The gasifier’s performance and cold gas efficiency were calculated for three volumetric airflow rates: 7.16 m3/h, 5.97 m3/h, and 4.78 m3/h. The efficiency was found maximum of 69.6% for an airflow rate of 7.16 m3/h. The oxidation zone and neck region of the gasifier reactor had the maximum recorded temperatures of 845 °C and 823 °C for Swietenia macrophylla (SM) and Mangifera indica (MI), respectively. A two-dimensional computational fluid dynamics (CFD) model for a …

Experimental Measurement Demonstration Of Initial And Steady-State Heights Of A Co2 Vertical Jet Fountain In Still Air, Brock Mcrae

Chemical Engineering Undergraduate Honors Theses

Atmospheric releases of heavier-than-air gases pose acute near-field hazards in low wind, yet the near-source behavior of vertical dense-gas jets in still air is sparsely documented. This thesis demonstrates a repeatable laboratory method for measuring the initial and steady-state rise heights of a carbon dioxide (CO₂) jet issued vertically from a floor nozzle in the CHRC ultra-low-speed wind tunnel under still-air conditions. Controlled mixtures (pure CO₂, 50/50 CO₂-air, 25/75 CO₂-air) were released at four flow rates (75, 100, 125, 150 LPM) with five repeats per condition. A fixed camera and tape-measure scale enabled video extraction of jet height using Kinovea …

Calibration And Validation Of Cyclic Stretching Platform For Stiffness Testing Of Engineered Heart Tissue, Jillian Welsh

Chemical Engineering Undergraduate Honors Theses

Cardiac fibrosis (CF) is a major contributor to the high mortality rate of heart failure which affects over 23 million people worldwide. CF involves cardiac fibroblasts depositing extracellular matrix, made primarily of collagen type I, which stiffens the heart walls over time. As testing new drugs for CF is deterred by the high cost and time required for animal and clinical trials, this research helps to solve the problem by providing an in vitro platform to study fibrosis.

The work involved creating tissues containing human cardiac fibroblast (HCFB) cells. Spring testing was conducted to determine the force applied by the …

Purifying Circular Rna Therapeutics, Karen Guillen-Cuevas

All Dissertations

Circular RNA (circRNA) has emerged as a promising therapeutic modality due to its enhanced stability and resistance to exonuclease degradation compared with linear mRNA. However, its stability and functionality depend strongly on achieving high purity, and current methods such as size-exclusion chromatography suffer from peak overlap, low yields, and poor scalability. This dissertation addresses these limitations by developing ultrafiltration-based strategies to purify circRNA directly from in vitro transcription (IVT) and self-splicing (circularization) reactions. First, the feasibility of ultrafiltration was demonstrated using polyethersulfone membranes, where sieving coefficients and critical flux values of circRNA, precursor RNA, and nicked RNA were measured to …

Advanced Membrane Methods For Treatment Of Toxic Contaminants And Education Of Early-Career Researchers, Thomas Mckean Iii

Graduate Theses and Dissertations

Membrane technologies are attractive as they are linearly scalable and often require lower operating costs compared to other technologies. One area where they are particularly effective is the treatment of water contaminated with toxic substances. The simplicity of this removal mechanism provides reliable performance capable of meeting stringent removal requirements. However, membrane fouling is a major challenge that often limits their commercial viability. This research sought to investigate novel approaches to pretreatment and membrane modification to overcome fouling and high operating pressures. In addition, membrane separation processes can often be developed into simple yet educational experiments that are ideal for …

Design Of Tertiary Z-Scheme Cuo–Zno@Mxene Photocatalyst With Superior Visible-Light Activity For Decomposition Of Nanoplastics, Ahmad Najafidoust, Javad Farahbakhsh, Roham Ghanbari, Mohadeseh Najafi, Alireza Keshavarz, Stefan Iglauer, Masoumeh Zargar

Research outputs 2022 to 2026

Plastic pollution has resulted in the widespread accumulation of nanoplastics (NPs) in aquatic environments, highlighting the need for innovative remediation strategies. In this study, a tertiary z-scheme CuO–ZnO/MXene nanocomposite was developed for the visible-light-assisted degradation of polyethylene-based NPs. ZnO particles were doped with 8 % CuO to form a p-n type heterojunction, and the resulting CuO–ZnO nanoparticles were anchored onto two-dimensional MXene sheets through a sono-precipitation dispersion method, forming a ternary z-scheme photocatalyst with enhanced surface area. The composite exhibited a low electron-hole recombination rate and a tunable band gap (~2.55 eV), enabling efficient visible-light absorption. Under visible light irradiation, …

Biopolymer-Based Membranes And Their Application In Per- And Polyfluorinated Substances Removal: Perspective Review, Roham Ghanbari, Ratish Permala, Stefan Iglauer, Masoumeh Zargar

Research outputs 2022 to 2026

Over the past 2 decades, per- and polyfluoroalkyl substances (PFAS) have attracted considerable attention due to their widespread occurrence and adverse effects on both the environment and human health. PFAS possess strong carbon‑fluorine bonds that confer exceptional chemical stability and resistance to natural degradation; their fluorinated chains are highly hydrophobic and oleophobic, while their polar functional groups contribute to amphiphilic behavior and water solubility. Certain PFAS thus pose serious risks to living organisms, especially mammals. Membrane filtration is recognized as a particularly effective technique, achieving removal efficiencies greater than 99 % for long-chain-PFAS (including anionic, cationic, and zwitterionic PFAS molecules). …

Developing A Metabolic Engineering Chassis From A Metabolically Versatile Organism, Rhodopseudomonas Palustris Cga009, Mark William Kathol

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Metabolic engineering has made it possible to develop biological processes that were once considered economically unfeasible or uncompetitive. This field has enabled both a robust niche market, whereby products, such as pharmaceutical substances like monoclonal antibodies, are produced exclusively through biological means, as well as an emerging commodity production market. Nonetheless, significant challenges remain in harnessing the unique metabolic capabilities of genetically non-tractable organisms. This dissertation focuses on Rhodopseudomonas palustris CGA009 as a potential chassis for metabolic engineering. To address the issue of genetic intractability, a synthetic biology toolkit was developed to facilitate the expression of heterologous proteins in R …

Breaking Through The Strength-Ductility Trade-Off Of Lpbf-Produced Ti-Xnb Alloys From Mixed Powders Via Ω-Phase Induced Heterostructure, Jian Bo Jin, Shengfeng Zhou, Huan Yang, Junjie Yang, Zhiguo Zhang, Baisong Guo, Lai Chang Zhang

Research outputs 2022 to 2026

Highlights Powder-mixed Ti-35Nb alloy produced by LPBF breaks through the strength-ductility trade-off. The ultimate strength, uniform elongation and Young’s modulus of heterostructured Ti-35Nb alloy are (806 ± 7) MPa, (18.0 ± 1.1)%, and (68 ± 6) GPa, respectively. In-situ laser re-melting improves elemental distribution uniformity in LPBF-produced powder-mixed Ti-xNb alloys. Optimized Nb content induces the phase transformation of β + α′ → β + ω in Ti-xNb alloy. Heterostructured β + ω/β enhances the strength-ductility synergy of Ti-35Nb alloy.

Process Intensification Of Essential Oils Extraction Using Instantaneous Controlled Pressure Drop Technology, Muhammad Hassnain, Mohammed Ilyas Khaledi, Muhammad Rizwan Azhar

Research outputs 2022 to 2026

Essential oils (EOs) are volatile, hydrophobic compounds derived from plants, widely used in food, cosmetic, pharmaceutical, and fragrance industries for their bioactive potential and associated health benefits. Traditional EO extraction methods are limited by low yields, long processing times, and thermal degradation of sensitive bioactive compounds. Instantaneous Controlled Pressure Drop (CID) technology is an advanced intensification method to overcome these challenges by applying compression-decompression cycles and modifying plant structure to enhance heat and mass transfer. This review provides a comprehensive analysis of CID technology and outlines its mechanism and principles. It compiles global research on CID applied to different plants …

The Microbial Factor In Subsurface Hydrogen Behavior: Implications For Wettability And Interfacial Dynamics, Hamid Esfandyari, Raziallah Jafari Jozani, Aliakbar Hassanpouryouzband, Farhid Hemmatzadeh, Manouchehr Haghighi, Stefan Iglauer, Alireza Keshavarz, Abbas Zeinijahromi

Research outputs 2022 to 2026

Microbial activity plays a significant role in subsurface hydrogen behavior, with implications for underground storage and natural hydrogen systems. This study examines how microbial processes influence the wettability and solid–liquid interfacial characteristics of key subsurface minerals, calcite, dolomite, quartz, and gypsum, in hydrogen-brine-rock systems under realistic subsurface conditions. Wettability directly affects hydrogen distribution, flow dynamics, and trapping, making it a critical factor for both storage and natural recovery applications. Experiments were conducted under high-pressure, high-temperature conditions, with SEM/EDS analysis used to characterize interfacial modifications and mineral surface changes across three scenarios: clean, organic acid-aged, and microbial-aged. Microbial aging consistently increased …

Sulfurized Serpentine And Perovskite Assisted Graphitic Carbon Nitride Nanocomposites For Photocatalytic Green Hydrogen Production, Yahia Alanizan

Thesis/ Dissertation Defenses

Green hydrogen production has received great attention in recent years as a solution to mitigate greenhouse gas emissions resulted by burning fossil fuels. This thesis focuses on development of photocatalytic water splitting green hydrogen production under visible light irradiation of g-C3N4 based sulfurized naturally occurring serpentine rock and oxysulfide perovskite nanocomposites. This work reveals the effect of partial sulfurization of co-catalysts to boost charge generation, charge transfer, and light absorption. The presented work determined the potential of sulfurizing naturally occurring rocks and minerals acting as support and enhanced co-catalysts that exhibit narrower band gaps, with sulfur rich sites for visible …

Development Of A Digestion Procedure Using Fe2+ Ions For Electrochemical Detection Of Mno2 Particles In Drinking Water, Kayla Elliott, Sarah Jane Payne, Zhe She

Journal of Electrochemistry

Developing methods for detection contaminants in drinking water is essential to ensuring that safe and acceptable quality drinking water is delivered to consumers. While manganese (Mn) was previously known only as a mere aesthetic issue, recent epidemiological data has shown to have negative neurological effects on humans, especially on children, prompting new health-based guidelines by Health Canada and the World Health Organization. In drinking water, Mn exists predominantly as Mn(II) and Mn(IV), and is regulated based on total Mn levels. Interestingly, measurement of Mn particulate using electroanalytical methods has not yet been reported in the literature. Herein, a digestion procedure …