Biomedical Engineering and Bioengineering Commons^™

Systematic Development Of Ionizable Lipid Nanoparticles For Placental Mrna Delivery Using A Design Of Experiments Approach, Rachel E. Young, Katherine Nelson, Samuel I. Hofbauer, Tara Vijayakumar, Mohamad-Gabriel Alameh, Drew Weissman, Charalampos Papachristou, Jason P Gleghorn, Rachel S. Riley

Henry M. Rowan College of Engineering Departmental Research

Ionizable lipid nanoparticles (LNPs) have gained attention as mRNA delivery platforms for vaccination against COVID-19 and for protein replacement therapies. LNPs enhance mRNA stability, circulation time, cellular uptake, and preferential delivery to specific tissues compared to mRNA with no carrier platform. However, LNPs are only in the beginning stages of development for safe and effective mRNA delivery to the placenta to treat placental dysfunction. Here, we develop LNPs that enable high levels of mRNA delivery to trophoblasts in vitro and to the placenta in vivo with no toxicity. We conducted a Design of Experiments to explore how LNP composition, including …

Adjuvant Pluronic F68 Is Compatible With A Plant Root-Colonizing Probiotic, Pseudomonas Chlororaphis O6, Amanda R. Streeter, Anthony Cartwright, Mohammad Zargaran, Anagha Wankhade, Anne J. Anderson, David W. Britt

Biological Engineering Faculty Publications

Plant probiotic bacteria are being increasingly used to maximize both the productivity and quality of field crops. Pseudomonas chlororaphis O6 (PcO6) is a plant root colonizer with probiotic activities. This bacterium produces an array of metabolites, including a group of phenazines that are functional in plant protection. The paper reports responses of PcO6 to a nonionic triblock copolymer surfactant, Pluronic F68. This Pluronic exhibits membrane "healing" activity and improves cryopreservation recovery in eukaryotic cells. The product is FDA-approved and is applied as an adjuvant in formulations used in agriculture, medicine, and biotechnology. Growth of PcO6 on …

Getting Off On The Right Foot: Addressing Severe Lymphedema Through A Novel Shoe Design, Carl R. Russell Iii, Anjollie M. Ramakrishna, Christopher H. Johns Iii, Alana V. Van Wijnen

Purdue Journal of Service-Learning and International Engagement

Engineering Projects in Community Service (EPICS) is a service-learning design program run by the College of Engineering at Purdue University. EPICS teaches students design skills by providing solutions for individuals, communities, and organizations in the surrounding area while mirroring engineering industry standards. Biomedical Engineering is a team within EPICS that strives to serve community partners through biomedical applications. HR is a patient who suffers from severe lymphedema. This condition results in her foot swelling three times its original size and requires her to utilize weekly leg compression therapy. Prescription shoes are slightly adequate. However, they lead to sores and pain …

The Role Of Interactive Spaces In Enhancing Social Interaction Of Children In Orphanage Institutions, Zainab Khalid Rashad Al Khafaji, Shamael M. W. Al Dabbagh

Al-Esraa University College Journal for Engineering Sciences

The child who moves to an environment outside his family environment, suchas orphanage institutions because of the loss of one or all of his family members, itis obvious that he feels a kind of alienation and isolation from his peers and a loss ofa sense of interaction and communication with society, so it is possible to mitigatethis feeling by promoting diversity in interactive activities within the internal spacesof the orphanage institutions and at the neighborhood level, the research problemwas represented by "the lack of clarity in the nature of knowledge aboutthe role of of interactive spaces in enhancing social interaction …

Environmental Footmark And Its Effects On The Sustainability Of Towns, Ali Abdul Samea Hameed

Al-Esraa University College Journal for Engineering Sciences

The Ecological Footmark (EF) is one of the utmost recent and important criteria for assessing the capacity of the population to uphold a life sustainable way. It tests the extent which the indicators have an influence on the natural resources (NR) of the ecosystem such as forestry, water bodies, agricultural lands, pastures, CO2, and properties for construction purposes. This footmark was utilized to evaluate the use of NR to the capacity of the land for regeneration. This research paper discusses the theoretical conceptual component of the EF framework and plans regarding issues related to the environment. The environmental image (EI) …

The Effects Of Urban Fragmentation / Analysis Of The Causes And Challenges In The City Structure, Seemon Matte Abbo, Inaam Amin Albazzaz

Al-Esraa University College Journal for Engineering Sciences

Fragmentation is a fundamental term in the history of critical urban thought,yet the product of fragmentation is manifested in fragments. Urban fragmentationis used to describe the phenomenon that occurs when the urban environment isbroken up into smaller units or dispersed areas. This means that cities becomefragmented rather than cohesive and integrated. This disintegration can be the resultof various factors such as poor planning, lack of infrastructure, different land uses,and social dispersion. This research addresses the problem of urban fragmentationas one of the challenges facing the urban structure of cities, and clarifies the mostimportant factors affecting the city structure during the …

A Review: Special Types Of Concrete Used In Structures, Reem Hatem Ahmed, Mohammed Mosleh Salman

Al-Esraa University College Journal for Engineering Sciences

The term “special” possesses inherent significance and is utilized in this context to denote the distinctive attributes characteristic of concrete. The term “special” connotes enhanced strength, durability, and solidity in concrete. The production of specialized concrete involves the incorporation of distinct components via specific procedures following specific criteria. Specialized concrete is required to construct unique structures such as power plants, offshore buildings, docks, and airports. Each of these unique structures is essential for survival in harsh conditions, including sulfate, chemical, acid, and chloride attacks, as well as other surface characteristics. Concrete is a versatile material that exhibits good compressive strength …

A Computational Exploration: Docking Analysis Of Compounds From Foeniculum Vulgare As Potential Aromatase Inhibitors For Endometriosis Candidate Therapy, Dwi Anita Suryandari, Puji Sari, Hadi Sunaryo, Khaerunissa Anbar Istiadi

Indonesian Journal of Medical Chemistry and Bioinformatics

Aromatase inhibitors (AI) have controlling symptoms and size of endometriotic implants, making them a promising second-line therapy for endometriosis treatment.pretreatment with letrozole, an AI, combined with leuprolide acetate and resveratrol has been found to improve in vitro fertilization (IVF) outcomes in women mild endometriosis.in this study we screening and analysis of ten phenolic compounds from Foeniculum vulgare using molecular docking with Mcole server.from this results showed that three phenolic trans resveratrol (TR), caempherol coumaril (CC) have low gibbs energy compare with resveratrol (R). The binding modalities of compound TR and compound R were hydrogen-bonding between the hydroxyl and oxygen atom …

The Role Of Young Architects As Entrepreneurship Generational Units In Shaping The Future Of Architecture, Attaa H. Al Obaidi, Anwar. S. Al-Qaraghuli

Al-Esraa University College Journal for Engineering Sciences

Youth are the pillar of nations and the secret of their renaissance, thebuilders of their civilization and the line of defense, the main participants inbasic planning processes, and the agents of change to build a world that iscompatible with the aspirations of future generations. This applies to the field ofarchitecture in terms of the importance of young architects in shaping it underthe challenges it faces, which impose interaction in a manner commensuratewith the emerging requirements and needs. This research is based on theconsideration that young architects are an integrated phenomenon thatcontributes to shaping architectural production according to the data of …

Using Artificial Intelligence To Diagnose Demented In The Elderly, Ohood Fadil Alwan

Al-Esraa University College Journal for Engineering Sciences

In addition to possible other symptoms, memory loss and impairment are the hallmarks of Demented or Alzheimer’s disease (AD). Despite the fact that dementia is incurable and has a significant negative impact on patients' lives, an early diagnosis can help start the right treatment and prevent additional brain damage. Over the years, machine learning techniques have been used to classify AD; nevertheless, the efficacy of the results depends on the use of multi-step classifiers and manually created features. Thanks to recent advances in deep learning, patterns may now be classified using neural networks' final stage. In order to diagnose dementia …

Risk Detection Of Gestational Diabetes In Early Pregnancy Depend On Artificial Neural Network, Sanaa Hammad Dhahi

Al-Esraa University College Journal for Engineering Sciences

Early prediction of gestational diabetes mellitus (GDM) risk is particularly important because it may enable more effective interventions and reduce cumulative maternal and fetal injury. The aim of this study is to develop machine learning (ML) models for early prediction of GDM using widely available variables, facilitating early intervention, and enabling the application of prediction models in settings where more complex tests are not accessible. In this paper, an artificial neural network (ANN) was used to detect the risk of gestational diabetes. A global dataset was used to collect clinical and experimental data from pregnant women and people with diabetes …

Impact Of Merging Basalt Fibers With Various Types Of Concrete And The Factors Affecting Them: A Review, Adil M. Jabbar, Lubna S. Dankha

Al-Esraa University College Journal for Engineering Sciences

This paper reviews effecting of blending basalt fibers into concreteon mechanical properties, ductility, and toughness. Based on concretecomponents, the perfect basalt fiber percentage can differ and is influencedby factors like fiber length, w/c ratio, and age. The beneficial basalt fiberspercentage is lower than that of steel fibers. Even with a low concentrationof basalt fibers, concrete tensile and flexural strength can be improved moreeffectively than a higher amount of steel fibers. As the amount of basaltfiber increases, the mix workability decreases. Additionally, the flow abilitymay drop slightly as the length of the fibers increases. Adding basalt fiber toconcrete can minimize drying …

Machine Learning And Metabolic Model Guided Crispri Reveals A Central Role For Phosphoglycerate Mutase In Chlamydia Trachomatis Persistence, Niaz Bahar Chowdhury, Nick Pokorzynski, Elizabeth A. Rucks, Scot P. Ouellette, Rey A. Carabeo, Rajib Saha

Department of Chemical and Biomolecular Engineering: Faculty Publications

Upon nutrient starvation, Chlamydia trachomatis serovar L2 (CTL) shifts from its normal growth to a non-replicating form, termed persistence. It is unclear if persistence is an adaptive response or lack of it. To understand that transcriptomics data were collected for nutrientsufficient and nutrient-starved CTL. Applying machine learning approaches on transcriptomics data revealed a global transcriptomic rewiring of CTL under stress conditions without having any global stress regulator. This indicated that CTL’s stress response is due to lack of an adaptive response mechanism. To investigate the impact of this on CTL metabolism, we reconstructed a genome-scale metabolic model of CTL (iCTL278) …

Colloid Fabrication For Biomedical Engineering Applications Through Droplet Microfluidics, Lewis I. Larsen

Biomedical Engineering ETDs

The goal of this work was to leverage soft colloid fabrication through droplet microfluidics to improve the biomedical fields of radiotracer microspheres for selective internal radiation therapy (SIRT) and 3D hydrogel tissue constructs for cell culture with future potential as a platform to study liver diseases and drug response. The importance of positron emission tomography (PET) imaging microspheres infused with positron-emitting radioisotopes such as Ga-68, Cu-64, and F-18 is that they accurately map tumor-to-liver uptake (TLR) ratio and lung shunting factor of hepatocellular carcinoma. These pretreatment radiotracers model the intravascular accumulation behavior of radioactive therapeutic Y-90 SIRT microspheres. Qualifying patients, …

Air-Jet Bioprinting Of Stem Cell-Encapsulated Alginate Micro-Droplets For Organoid Biomanufacturing, Mirabella B. Stump

Electronic Theses and Dissertations

Biomanufactured 3D-organoids show promise for modeling and treating various conditions, particularly insulin-dependent diabetes mellitus (type-1 diabetes). Pancreatic islet organoids serve as sustainable sources of insulin-producing β-cells, potentially restoring insulin regulation in patients and providing a long-term solution for diabetes. Hydrogel droplet printing is an emerging technique in regenerative medicine due to its advantageous tunability and biocompatibility; however, challenges of consistency, quality control, and scalability persist. To address these issues, we developed a novel air-jetting based droplet bioprinting system for the generation of monodispersed alginate micro-droplets. The objective of this project was to incorporate human induced pluripotent stem cells (hiPSCs) into …

Large - Scale Manufacturing Of Fluorescent Perfluorocarbon Nanoemulsions For Oxygen Delivery, Anneliese Troidle

Electronic Theses and Dissertations

Over 100,000 Americans are currently waiting for a life – saving organ transplant, yet 17 patients die daily waiting for a transplant they will never receive [1]. Preservation of organs is an area that has seen innovation throughout the years and can improve rates of transplantation, yet vascularized composite allotransplantation preservation has not followed suit [2]. Current standard of care is use of electrolyte solutions such as Histidine – Tryptophan – Ketoglutarate (HTK) or University of Wisconsin (UW) Solution, yet these perfusates lack an oxygen carrying component [3]. Hemoglobin – based oxygen carriers (HBOCs) …

Paper-Based Ratiometric Sensor Array For Pathogenic Bacterial Species Identification, Aayushi Laliwala

Theses & Dissertations

Bacterial infections are the leading cause of mortality worldwide due to the lack of accurate diagnosis of the pathogens causing infections. Currently, microbiological and molecular techniques are used to identify pathogenic bacteria. However, these techniques have limitations as they are time-consuming, cost-inefficient, and require trained personnel. Therefore, timely and precise identification of pathogenic bacteria is crucial for public safety. Our lab previously synthesized a fluorescent sensor array (3-hydroxyflavone derivatives) encapsulated in hyaluronic acid-based nanoparticles to identify bacterial species and recognize their Gram status. Though promising, this technique was relatively time-consuming and required high sample and volumes.

To overcome these limitations, …

Fabrication And Characterization Of Flexible Microelectrode Arrays On Kapton Polyimide For Implantable Spinal Cord Stimulation Applications, Nabil Jamhour

Theses and Dissertations

Recent advances in spinal cord stimulation (SCS) technology have made a significant impact in the treatment of chronic pain which is a prevalent issue amongst 20% of the population in developed nations. A few medical device manufactures have developed SCS devices that have been studied in clinical environments and reported to be effective and safe for commercial use. However, the current SCS devices available are bulky, rigid, and still present infection, fibrosis, discomfort, pulse delivery challenges and other concerns for patients. In this study, flexible electrodes processed on Kapton® polyimide films by physical vapor and inkjet deposition are fabricated and …

Vapor-Phase Synthesis Of Electrocatalytic Covalent Organic Frameworks, Syed Ibrahim Gnani Peer Mohamed, Shahriar Namvar, Tan Zhang, Hessam Shahbazi, Zhen Jiang, Andrew M. Rappe, Amin Salehi-Khojin, Siamak Nejati

Department of Chemical and Biomolecular Engineering: Faculty Publications

The inability to process many covalent organic frameworks (COFs) as thin films plagues their widespread utilization. Herein, a vapor-phase pathway for the bottom-up synthesis of a class of porphyrin-based COFs is presented. This approach allows integrating electrocatalysts made of metal-ion-containing COFs into the electrodes’ architectures in a single-step synthesis and deposition. By precisely controlling the metal sites at the atomic level, remarkable electrocatalytic performance is achieved, resulting in unprecedentedly high mass activity values. How the choice of metal atoms, i.e., cobalt and copper, can determine the catalytic activities of POR-COFs is demonstrated. The theoretical data proves that the Cu site …

Survey Of Transfer Learning Approaches In The Machine Learning Of Digital Health Sensing Data, Lina Chato, Emma Regentova

Electrical & Computer Engineering Faculty Research

Machine learning and digital health sensing data have led to numerous research achievements aimed at improving digital health technology. However, using machine learning in digital health poses challenges related to data availability, such as incomplete, unstructured, and fragmented data, as well as issues related to data privacy, security, and data format standardization. Furthermore, there is a risk of bias and discrimination in machine learning models. Thus, developing an accurate prediction model from scratch can be an expensive and complicated task that often requires extensive experiments and complex computations. Transfer learning methods have emerged as a feasible solution to address these …