Biomedical Engineering and Bioengineering Commons^™

Effects Of An Indole Derivative On Cell Proliferation, Transfection, And Alternative Splicing In Production Of Lentiviral Vectors By Transient Co-Transfection, Nataly Carolina Mier, Donald Keith Roper

Biological Engineering Faculty Publications

Lentiviral vectors derived from human immunodeficiency virus type I are widely used to deliver functional gene copies to mammalian cells for research and gene therapies. Post-transcriptional splicing of lentiviral vector transgene in transduced host and transfected producer cells presents barriers to widespread application of lentiviral vector-based therapies. The present study examined effects of indole derivative compound IDC16 on splicing of lentiviral vector transcripts in producer cells and corresponding yield of infectious lentiviral vectors. Indole IDC16 was shown previously to modify alternative splicing in human immunodeficiency virus type I. Human embryonic kidney 293T cells were transiently transfected by 3^rd generation …

Molecular Behavior Of Silicone Adhesive At Buried Polymer Interface Studied By Molecular Dynamics Simulation And Sum Frequency Generation Vibrational Spectroscopy, Yuchen Wu, Ting Lin, Elizabeth Santos, Dongchan Ahn, Ryan Marson, Pranab Sarker, Xiaoyun Chen, Frédéric Gubbels, Nick E. Shephard, Carol Mohler, Tao Wei, Tzu-Chi Kuoc, Zhan Chen

Faculty Publications

Silicones have excellent material properties and are used extensively in many applications, ranging from adhesives and lubricants to electrical insulation. To ensure strong adhesion of silicone adhesives to a wide variety of substrates, silane-based adhesion promotors are typically blended into the silicone adhesive formulation. However, little is known at the molecular level about the true silane adhesion promotion mechanism, which limits the ability to develop even more effective adhesion promoters. To understand the adhesion promotion mechanism of silane molecules at the molecular level, this study has used sum frequency generation vibrational spectroscopy (SFG) to determine the behavior of (3-glycidoxypropyl)trimethoxy silane …

Single-Molecule Orientation Imaging Reveals The Nano-Architecture Of Amyloid Fibrils Undergoing Growth And Decay, Brian Sun, Tianben Ding, Weiyan Zhou, Tara S. Porter, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Amyloid-beta (Aβ42) aggregates are characteristic Alzheimer’s disease signatures, but probing how their nanoscale architectures influence their growth and decay remains challenging using current technologies. Here, we apply time-lapse single-molecule orientation-localization microscopy (SMOLM) to measure the orientations and rotational “wobble” of Nile blue (NB) molecules transiently binding to Aβ42 fibrils. We correlate fibril architectures measured by SMOLM with their growth and decay over the course of 5 to 20 min visualized by single-molecule localization microscopy (SMLM). We discover that stable Aβ42 fibrils tend to be well-ordered and signified by well-aligned NB orientations and small wobble. SMOLM also shows that increasing order …

A Study On The Effects Of Cementless Total Knee Arthroplasty Implants’ Surface Morphology Via Finite Element Analysis, Scott Hazelwood, Peter J. Hunt, Mohammad Noori, Naudereh B. Noori, Wael A. Altabey

Biomedical Engineering

Total knee arthroplasty (TKA) is one of the most commonly performed orthopedic surgeries, with nearly one million performed in 2020 in the United States alone. Changing patient demographics, predominately indicated by increases in younger, more active, and more obese patients undergoing TKA, poses a challenge to orthopedic surgeons as these factors present a greater risk of long-term complications. Historically, cemented TKA has been the gold standard for fixation, but long-term aseptic loosening continues to be a risk for cemented implants. Cementless TKA, which relies on the surface morphology of a porous coating for biologic fixation of implant to bone, may …

Noninvasive Technique For Measuring Cardiac Output, Grace E. Kolar, Rachel Simonton, Eli Strohecker

Williams Honors College, Honors Research Projects

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Evaluating Visual Dependence In Postural Stability Using Smartphone And Stroboscopic Glasses, Brent A. Harper, Michael Shiraishi, Rahul Soangra

Physical Therapy Faculty Articles and Research

This study explores the efficacy of integrating stroboscopic glasses with smartphone-based applications to evaluate postural control, offering a cost-effective alternative to traditional forceplate technology. Athletes, particularly those with visual and visuo-oculomotor enhancements due to sports, often suffer from injuries that necessitate reliance on visual inputs for balance—conditions that can be simulated and studied using visual perturbation methods such as stroboscopic glasses. These glasses intermittently occlude vision, mimicking visual impairments that are crucial in assessing dependency on visual information for postural stability. Participants performed these tasks under three visual conditions: full vision, partial vision occlusion via stroboscopic glasses, and no vision …

Development Of An Advanced Step Counting Algorithm With Integrated Activity Detection For Free Living Environments, Paige M. Dolan

Master's Theses

Physical activity plays a crucial role in maintaining overall health and reducing the risk of various chronic diseases. Step counting has emerged as a popular method for assessing physical activity levels, given its simplicity and ease of use. However, accurately measuring step counts in free-living environments presents significant challenges, with most activity trackers exhibiting a percent error above 20%. This study aims to address these challenges by creating a machine learning algorithm that leverages activity labels to improve step count accuracy in real-world conditions. Two approaches to balancing data were used: one employed a simpler oversampling technique, while the other …

Engineering An Injectable Hydrogel With Self-Assembling 3d Vasculature, Kendyl Cohn

Master's Theses

This research developed methods for culturing self-assembling capillaries in an injectable gel as a potential method for vascularizing tissue-on-a-chip models to mimic physiological drug delivery. Additionally, a mathematical model was developed as a tool for understanding nutrient delivery and comparison of potential delivery systems. Organs-on-a-chip provide novel platforms for studying biology and physiology in 3D, allow exploration of tissue engineering on a manageable scale, and serve as models for drug screening and drug-delivery testing.

Methods were first developed for co-culture of endothelial cells and fibroblasts (3T3s or HDFs) in 2D, evaluating culture time, seeding density and ratio of HUVECs and …

Thermal Preconditioning Effects On Perforation Propensity Of Transvenous Implantable Cardiac Leads Used For Pacing And Defibrillation, Diane Muff

Master's Theses

Thermal preconditioning to simulate shipping, handling and storage conditions did not affect distal tip stiffness values for permanently implantable cardiac leads used for pacing and defibrillation. Leads that were subjected to extreme temperatures and temperature cycling did not show changes in buckle force values compared with control specimens which were maintained at ambient room conditions. Absolute differences between all measurements were small, generally under 0.05 N and were attributable to measurement variability.

Buckle force values are used to assess the propensity of leads to perforate the heart, a rare but potentially serious complication of implantable pacing and defibrillation systems. Since …

In-Vitro Hydrogel Blood Clots, Ethan Evans, Khoi-Austin Ngo

Bioengineering Senior Theses

Creating a sustainable, ethical, and affordable substitute for animal blood in balloon catheter testing for Deep Vein Thrombosis (DVT) is important to take into consideration. This research utilizes agarose and dextran to develop hydrogel-based artificial blood clots. These hydro gels replicate the mechanical characteristics of human thrombi across acute, subacute, and chronic stages through their elastic and viscous moduli (G' and G") and phase angles. This project addresses extant testing protocols, including temperature regulation, agarose processing, and well plate size, and suggests efficient approaches to encounter these problems.

The hydrogels were characterized by means of a thorough literature review and …

Packed-Bed Reactor For Integrated Peritoneal Dialysis, Julia Appleget, Ally Kwong, Megan Moglia

Bioengineering Senior Theses

Patients with chronic kidney disease (CKD) have lost kidney function. 800,000 Americans have end-stage kidney disease resulting in one of the leading causes of mortality cross-listed with diabetes and heart disease. Over the past decade, the percentage of patients using peritoneal dialysis (PD) has nearly doubled. PD which is a portable treatment involves the insertion of a catheter into the selectively permeable peritoneal cavity infused with a hyperosmolar dialysis fluid drawing the toxins from capillary beds of the peritoneum and into a gravity fed waste solution with a reported clearance of 87%. In order to improve the clearance of the …

Technocene, Vir Joseph Naidu

Masters Theses

Embodied human communication within the Anthropocene. Existing at the intersection of technology, and the body.

The design industry has developed technology that is, paradoxically, isolating. The exposure to a vast audience in the digital sphere has introduced new societal pressures, leading to a disconnection from our immediate surroundings, detached, and donning metaphorical masks. Technocene lives on the fringes of the discipline by blending conceptual thinking with practical application. Through curious, experimental artifacts, it prompts us to shed our masks and embrace vulnerability. Technocene endeavors to reimagine the human experience by acting as a discursive design project. It probes the boundaries …

Advances In Bone And Orthopedics 2024 Edition, Jean-Philippe Berteau, Laurent Pujo-Menjouet

Publications and Research

We are delighted to present the latest edition of ”Advances in Bone and Orthopedics,” a comprehensive compilation of pioneering research and innovative developments in the field. This volume brings together a selection of peer-reviewed abstracts from BONITOS 2024, the Fourth Season of the Bone and Orthopedics Interdisciplinary Symposium, held on May 31st, 2024, at the College of Staten Island in New York. This symposium, a hallmark event in the calendar of professionals dedicated to bone health and orthopedic excellence. The contributions within this volume reflect the breadth and depth of research presented at BONITOS 2024. These abstracts represent cutting-edge studies …

Smart Cushion Device For Elderly Aging In Place, Anna Dion, Nate Colley, Leonardo Bravo

Biomedical Engineering

Our task at hand is to develop a consumer-focused solution for improving elder care to provide additional peace of mind for seniors and caregivers using technology to extend independent living. We intend to create a device that will improve the quality of life of elderly individuals living alone by encouraging periodical movement throughout the day, along with monitoring pressure distribution and alerting when pressure concentration is potentially harmful. It will also alert users to the presence of moisture which can arise from spills or incontinence. The key stakeholders of this project include elderly adults over the age of 65 who …

Aerobic Exercise Improves Depressive Symptoms In The Unilateral 6-Ohda-Lesioned Rat Model Of Parkinson's Disease, Hannah Loughlin, Jacob Jackson, Chloe Looman, Alayna Starll, Jeremy Goldman, Zhiying Shan, Chunxiu Yu

Michigan Tech Publications

Aerobic exercise has been shown to have established benefits on motor function in Parkinson's disease (PD). However, the impact of exercise on depressive symptoms in PD remains unclear. This study aimed to investigate the effects of regular exercise, specifically using a forced running wheel, on both motor performance and the prevalence of depression in a unilateral 6-OHDA-lesioned rat model of PD. The behavioral outcomes of exercise were assessed through the rotarod test (RT), forelimb adjusting step test (FAST), sucrose consumption test (SCT), and novelty sucrose splash test (NSST). Our data revealed evident depressive symptoms in the PD animals, characterized by …

Prosthetic Forearm Controller, Claire Tylutki, Kevin Lopatka, Sofia Zaletel, Hardit Singh

Electrical Engineering

The EMG controlled prosthetic arm project represents a significant advancement in upper limb prosthetics, leveraging cutting-edge technology to address critical user needs. With over two million individuals in the United States living with limb loss, a substantial portion being upper limb amputees, the demand for accessible and functional prosthetic solutions is pressing. This project focuses largely on refining prosthetic limb control through the integration of electromyography (EMG) signal processing and control. Using surface electrodes, this project employs non-invasive means of data collection combined with modern digital signal processing methods to implement faster and more accurate control. The control system also …

Bolin Symes Prosthetic, Samuel C. Collins, Rowan Sun, Jake Hacker

Biomedical Engineering

This project’s purpose is to design a comfortable prosthetic for an individual with a Syme’s amputation, which is a below-knee amputation that removes the foot from the leg at the ankle joint. The goal is to minimize the discomfort that is associated with the current prosthetic leg options for the client, Mr. Bolin, who has the Syme’s amputation. This project also seeks to improve mobility, quality of life, and activity levels of the client. The stakeholder for this project is Mr. Bolin. The final prototype performed successfully when worn by the client and passed comfort testing. Furthermore, COMSOL modeling of …

Foot And Ankle Exoskeleton, Janet M. Velasquez Cabral, Kathryn C. Hamilton, Grant R. Lautze

Biomedical Engineering

This paper presents a non-motorized foot and ankle exoskeleton to assist persons who have no lower limb or abdominal function. Exoskeletons are defined as “an artificial external supporting structure” [1], they are wearable devices that can be used to assist the capabilities of the human body. Although the exoskeleton must be used with crutches, plantarflexion and dorsiflexion will be implemented within the ankle joint to alleviate strain on the foot and ankle, strategically designed in a way that supports the user so their walking performance is as ideal as possible. Additionally, safety straps will be integrated into the foot portion …

Prosthetic Aid For Lifting (Pal), Emma Caringella, Cameron Yartz, Dawn Veditz

Biomedical Engineering

Approximately 1.7 million people in the United States use a limb prosthetic [1]. For these individuals, their prosthetic often enables them to engage in their choice of physical activities. Many prosthetic companies design prosthetic limbs for use in specific athletic activities such as weightlifting. These devices are not designed for everyday wear or a large variety of tasks but enable the person to interact with free weights and workout machines. According to an email conversation between the team and Bob Radocy, Founder of Therapeutic Recreational Services (TRS Inc.) and upper limb amputee, while he could not reveal how many people …

Deep Vein Thrombosis Diagnostic Device, Alisha Mitha, Karina Martel, Ayla Silbahar

Biomedical Engineering

Deep Vein Thrombosis is one of the leading causes of death in hospitals worldwide due its ability to form in patients who are sedentary after receiving care. There is a need for a DVT diagnostic device that can be worn constantly in order to alert medical professionals if a thrombosis is forming, which is the basis of this senior project. The current solutions are time consuming, and look for thrombi after they have fully formed. The goal of this senior project is to design and construct a device to constantly monitor in-hospital patients for early stage DVT and alert hospital …