Biomedical Engineering and Bioengineering Commons^™

Validation Of Nanosecond Pulse Cancellation Using A Quadrupole Exposure System, Hollie A. Ryan

Biomedical Engineering Theses & Dissertations

Nanosecond pulsed electric fields (nsPEFs) offer a plethora of opportunities for developing integrative technologies as complements or alternatives to traditional medicine. Studies on the biological effects of nsPEFs in vitro and in vivo have revealed unique characteristics that suggest the potential for minimized risk of complications in patients, such as the ability of unipolar nsEPs to create permanent or transient pores in cell membranes that trigger localized lethal or non-lethal outcomes without consequential heating. A more recent finding was that such responses could be diminished by applying a bipolar pulse instead, a phenomenon dubbed bipolar cancellation, paving the way …

Using Natural Language Processing And Sentiment Analysis To Augment Traditional User-Centered Design: Development And Usability Study, Curtis L. Petersen, Ryan Halter, David Kotz, Lorie Loeb, Summer B. Cook, Dawna M. Pidgeon, Brock Christensen, John A. Batsis

Dartmouth Scholarship

Background: Sarcopenia, defined as the age-associated loss of muscle mass and strength, can be effectively mitigated through resistance-based physical activity. With compliance at approximately 40% for home-based exercise prescriptions, implementing a remote sensing system would help patients and clinicians to better understand treatment progress and increase compliance. The inclusion of end users in the development of mobile apps for remote-sensing systems can ensure that they are both user friendly and facilitate compliance. With advancements in natural language processing (NLP), there is potential for these methods to be used with data collected through the user-centered design process.

Objective: This study aims …

Decellularization Of Porcine Cartilage Promotes Chondrogenic Differentiation Of Human Chondrocytes, Roxanne Nicole Stone

Boise State University Theses and Dissertations

Knee osteoarthritis (knee OA) is the most common type of osteoarthritis (OA) and accounts for 70% of arthritis-related hospital admissions and 23% of clinical visits. Major limitations in both the current non-surgical and surgical methods are that they only relieve pain and show no evidence for restoring natural tissue anatomy. Leaders in the field propose that a stem cell treatment approach holds promise for the regeneration of a greater proportion of hyaline-like tissue at the repair site. (Cross et al., 2014; Escobar Ivirico, Bhattacharjee, Kuyinu, Nair, & Laurencin, 2017; Helmick et al., 2008; Toh, Foldager, Pei, & Hui, 2014).

It …

Development Of A Graphical User Interface For Ecg Signals Classification Using Statistical Features Analysis, Mousa Hammad S Aldosari

Theses and Dissertations

Cardiac diseases are the most common cause of mortality in the world. The detection of cardiac arrhythmias is not a straightforward process, since minor variations in the electrocardiogram (ECG) signals cannot be easily identified manually. Therefore, automatic detection and classification of cardiac arrhythmia would shorten the diagnostic time and accelerate medical intervention resulting in reducing the mortality rate. In this thesis, I have developed a simple and low-cost computer-aided diagnostic system using MATLAB-based Graphical User Interface (GUI) to facilitate fast operation and access to the data along with the overall accuracy of the system. The acquired ECG signals are processed …

An Experimental And Computational Mechanical Analysis Of Bone Anchors And Substrate Interface, Samantha May Schultz

Theses and Dissertations

Screw loosening is a common concern in orthopedic surgery. Therefore, this study is a computational and experimental evaluation of bone substrate and cortical screw interface. An axial load test was performed in an Instron with titanium alloy screws with 2.7 mm, 3.5 mm, and 4.5mm diameter orthopedic cortical screws inserted into SawBone with densities varying from 10 to 50 pounds per cubic foot (PCF), incrementing in sets of 10 PCF. SawBone was chosen in place of human bone because it has been shown to emulate the same mechanical properties (1). Twelve samples were prepared for each screw size and substrate …

Three-Dimensional (3d) Reconstruction Of Ultrasound Foetal Images Using Visualisation Toolkit (Vtk), Henry Dusim Grace Anabela

Student Works (2020-2029)

Three-dimensional (3D) reconstruction of two-dimensional (2D) ultrasound images has been used for easier analysing of anatomy related to abnormalities. 3D image reconstruction is required as it helps doctors interpret results and assess any defects quickly. Not only does it improve the accuracy of diagnosis, but it also saves a lot of time. In this thesis, 295 slices of 2D ultrasound images of a foetal are used to do 3D reconstruction using surface rendering, volume rendering, and multiplanar rendering technique. A similar software known as 3D Slicer is also used to reconstruct the ultrasound image for comparison purpose. VTK can easily …

Biomechanical Investigation Of Individual With Over-Pronation And Over-Supination Foot During Walking, Ghani Nur Saibah

Student Works (2020-2029)

Over-pronation and over-supination foot conditions are foot deformity that can lead to unstable ankle and subtalar joint during walking. They are define as condition where the foot roll in and out excessively from its neutral line and can lead to misalignment of the foot and leg in human in which can create abnormal gait cycle. Therefore, the purposes of this study are to analyze the foot-ankle joint biomechanical behavior including the range of motion, joint moment, joint power and GRF in a complete gait cycle for all subjects in this study and to compare the foot-ankle biomechanical behavior between normal …

Modeling, Fabrication And Characterization Of Coated Calcium Phosphate Scaffolds Produced By 3d-Printing For Tissue Engineering Application, Touri Maria

Student Works (2020-2029)

A fully interconnected 3-dimensional scaffold with pre-determined dimensions and porosity can be attained by design-dependent rapid prototyping techniques which possess precise control over the internal and external architecture of the scaffolds thus they can overcome the problems associated with the process-dependent classical approaches. Interruption of vascular flow with fracture or surgical osteotomy results in a transient hypoxic gradient within the wound and subsequent tissue necrosis. Tissue engineering scaffolds with oxygen generating elements have shown to be able to increase the level of oxygen and cell survivability. In this study, biphasic calcium phosphate (BCP) scaffolds with the composition of 60% hydroxyapatite …

Changes In Neural Network Connectivity Of Normal Young Adults In Response To Power Wheelchair Trainer Using Eeg Coherence, Ruby Phung

Masters Theses

The ability to move and explore their surrounding environment plays a critical role in the development of cognitive function in children, especially during early childhood. The lack of independent and autonomous mobility is, therefore, a clear disadvantage for the overall development of children with multiple, severe disabilities. Limited number of studies have been conducted on the impact of power mobility device on this specific population. Previous exploratory and pilot studies showed promising results regarding quantifiable and consistent changes in the electroencephalogram (EEG) of children with multiple, severe disabilities when provided with power mobility training. This study aimed to further extend …

Analysis Of Center Of Pressure And Center Of Mass To Determine Patterns Of Postural Stability In Neurotypical Subjects, Natalie Tipton

Masters Theses

The body’s postural control mechanism is responsible for responding to perturbations of balance and keeping the body upright. One of the main ways that this is completed during quiet standing, where both feet are planted on the ground, is through center of pressure oscillations. In these oscillations, the center of pressure circles around the center of mass, constantly counteracting any lean that exists in the body. These oscillations can be recorded with floorembedded force plates and center of mass can be recorded with a motion capture system. In this research, these signals were recorded for stances with feet together and …

Three-Dimensional (3d) Reconstruction Of Computed Tomography (Ct) Abdominal Images Using Visualization Toolkit (Vtk), Mat Saad Afifah Husna

Student Works (2020-2029)

A computerized tomography (CT) or computerized axial tomography (CAT) scan combines data from several X-raysito produce a detailed image of structures inside the body. CT scans produce 2D of a “slice” or section of the abdomen but the data can also be used to construct 3-dimensional images. However, CT scan unable to accurately represent the internal structure of soft tissues and soft-tissue lesions. Therefore, using the program in Visualization Tool Kit (VTK) is needed to construct a much more clearer 3D image. To reconstruct 3D images from 2D images of a “slice” or section of abdomen which will be resulting …

3-Dimensional Carbon Micro-Electro Mechanical System (Mems) Electrode For Potential Dielectrophoretic Hepatic Cell Patterning Application, Wan Yahya Wan Nurlina

Student Works (2020-2029)

The maintenance of liver tissue unique pattern remains a great challenge in the generation of functional engineered liver tissue in vitro. This thesis presents a carbon dielectrophoresis (DEP) based cell patterning for generating hepatic cell constructs, mimicking biological hepatic lobule. A new design of electrode named interdigitated radiating-strips electrode (IRSE) was simulated and optimized using COMSOL Multiphysics 4.2a. The simulation result proved that IRSE is capable to provide uniform distribution of electric field for generating three-dimensional (3D) DEP force. The use of carbon electrodes has been an alternative to traditional metal electrodes and insulator structures for DEP applications since more …

Enhancement Of Graphene Biocompatibility As A Potential Nano-Carrier For Drug Delivery Applications Using Natural Deep Eutectic Solvent, Zainal Abidin Mohamad Hamdi

Student Works (2020-2029)

Graphene has attracted massive interest in numerous biomedical applications such as anti-cancer therapy, drug delivery, bio-imaging and gene delivery. Therefore, it is important to ensure that graphene is nontoxic, and that its cellular biological behavior is safe and biocompatible. Herein, a new route was used to enhance the biocompatibility of graphene as a nano-carrier for potential drug delivery applications, using different compositions of natural deep eutectic solvents (DESs) as functionalizing agents, owing to their capability to introduce various functional groups and surface modifications. To meet this end, eight different combinations of binary and ternary DESs were synthesized using choline chloride …

Detection Of Standing And Sitting Variations Based On In-Socket Piezoelectric Sensors For Transfemoral Amputees, Alnusairi Tawfik Yahya

Student Works (2020-2029)

A transfemoral prosthesis is required to assist amputees to perform activities of daily living (ADL). The purely mechanical or passive prosthesis has some drawbacks such as consumption of high metabolic energy and limitations in mimicking normal dynamics and kinematics of gait pattern. In contrast, the active prosthesis offers better performance and consumes less metabolic energy. However, recent active prosthesis uses surface electromyography as its sensory system which requires massive preparation work, causes sores to the patient by its electrodes and requires a lot of computation to extract meaningful features. This thesis focuses on developing signal conditioning circuitry to classify six …

Characterization Of Chinese Hamster Ovary Cell Performance With Pentanoic Acid And N-Acetyl Cysteine Treatment, Joseph Camire

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To improve the cost of producing protein therapeutics manufacturers can attempt to increase the amount of protein produced per unit volume. Some small fatty-acids have shown the ability to increase protein concentrations in some manufacturing systems. An example system is in the industrially important cell line derived from a Chinese Hamster Ovary (CHO). A drawback of adding these fatty-acids is they can increase the rate of cell death.

A potential way to minimize the cell death rate, caused by adding fatty-acids, is to also add additional anti-oxidants. Through a series of studies, we demonstrate production with different fatty-acids, butanoic acid …

Model-Based Approach For Diffuse Glioma Classification, Grading, And Patient Survival Prediction, Zeina A. Shboul

Electrical & Computer Engineering Theses & Dissertations

The work in this dissertation proposes model-based approaches for molecular mutations classification of gliomas, grading based on radiomics features and genomics, and prediction of diffuse gliomas clinical outcome in overall patient survival. Diffuse gliomas are types of Central Nervous System (CNS) brain tumors that account for 25.5% of primary brain and CNS tumors and originate from the supportive glial cells. In the 2016 World Health Organization’s (WHO) criteria for CNS brain tumor, a major reclassification of the diffuse gliomas is presented based on gliomas molecular mutations and the growth behavior. Currently, the status of molecular mutations is determined by obtaining …

A Novel Non-Enzymatic Glucose Biofuel Cell With Mobile Glucose Sensing, Ankit Baingane

Electrical & Computer Engineering Theses & Dissertations

Herein, we report a novel non-enzymatic glucose biofuel cell with mobile glucose sensing. We characterized the power generation and biosensing capabilities in presence of glucose analyte. This system was developed using a non-enzymatic glucose biofuel cell consisting of colloidal platinum coated gold microwire (Au-co-Pt) employed as an anode and the cathode which was constructed using a Gas diffusion electrode (GDE) with a platinum catalyst. The non-enzymatic glucose biofuel cell produced a maximum open circuit voltage of 0.54 V and delivered and a maximum short circuit current density of 1.6 mA/cm2 with a peak power density of 0.226 mW/cm2 at a …

Mechanical And Material Properties Of Nitinol And Its Application To Stents, Ariana Solis Eichler

Theses and Dissertations

Nitinol is a functional material with superelastic and shape memory properties derived from its unique molecular structure. The purpose of this thesis is to explore how the material and mechanical properties of self-expanding Nitinol stents affects the forces applied to arterial walls during stent deployment compared to the traditional balloon-expanding stainless steel stents, in addition to contrasting thin and thick-walled pressure vessel mathematical models. Nitinol’s mechanical properties can be optimized for stent applications by tailoring its processing procedures. Nitinol stents demonstrated a reduced circumferential hoop stress on the vessel wall and greater factor of safety with respect to vessel rupture …

A Computational Framework To Model Mesenchymal Stem Cell Nucleus Mechanics Using Confocal Microscopy, Zeke Kennedy

Boise State University Theses and Dissertations

The mechanical properties of the cell nucleus are emerging as a key component in genetic transcription. It has been shown that the stiffness of the nucleus in part regulates the transcription of genes in response to external mechanical stimuli. The stiffness has been shown to change as a result of both disease and changes to the external environment. While the mechanical structure of the nucleus can be visually documented using a confocal microscope, it is currently impossible to test the stiffness of the nucleus without a mechanical testing apparatus such as an atomic force microscope. This is problematic in that …

Novel Three-Dimensional Scaffold For Skeletal Muscle Tissue, Matthew H. Clegg

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cells are traditionally grown on a flat, plastic surface that has been optimized for cell attachment. While this is appropriate for growing and maintaining cells, this two-dimensional cell culture model is not an adequate representation of a three-dimensional body. Creating three-dimensional lab models is an important step to model diseases and damage to the skeletal muscle system.

To create this three-dimensional environment, a plastic frame was designed to support silkworm fibers. Skeletal muscle cells were then grown along the silkworm fibers and the growth of these cells in a three-dimensional environment was compared to cells grown on a flat, two-dimensional …