Microbiology Commons^™

Establishing A National Shellfish Sanitation Program In The Gambia, West Africa, Michael A. Rice, Foday Conteh, Karen Kent, Brian Crawford, Banja Bamba, Fatou Janha, Ismaila Bojang

Michael A Rice

A successful national program to assure sanitary quality of molluscan shellfish requires a multi-disciplinary and multi-agency governmental training, data collection, policy development and management effort in collaboration with members of the shellfish industry. The Tanbi Wetlands and other estuaries of Gambia support shellfisheries for oysters, Crassostrea tulipa, and the senile ark, Senelia senilis, conducted by the TRY Oyster Women’s Association. With low shellfish prices and a small local market, a Gambian National Shellfish Sanitation Program (GNSSP) was begun as a means to boost consumer confidence and allow market access to Gambia’s robust seasonal international tourism trade. Gambian officials began training …

N-Acetylglucosamine Regulates Virulence Properties In Microbial Pathogens, Shamoon Naseem, James B. Konopka

Department of Molecular Genetics and Microbiology Faculty Publications

There is growing evidence that the sugar N-acetylglucosamine (GlcNAc) plays diverse roles in cell signaling pathways that impact the virulence properties of microbes and host cells. GlcNAc is already well known as a ubiquitous structural component at the cell surface that forms part of bacterial cell wall peptidoglycan, cell wall chitin in fungi and parasites, and extracellular matrix glycosaminoglycans of animal cells. Chitin and peptidoglycan have been previously linked to cell signaling as they can stimulate responses in plant and animal host cells [1–3]. Recent studies now indicate that GlcNAc released from these polymers can also …

Antimicrobial Susceptibility Pattern And Biochemical Characteristics Of Staphylococcus Aureus: Impact Of Bio Field Treatment, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Sambhu Charan Mondal, Snehasis Jana

Mahendra Kumar Trivedi

Study background: Staphylococci are widespread in nature, mainly found on the skin and mucous membranes.Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of biofield treatment on antimicrobial susceptibility pattern and biochemical characteristics of S. aureus (ATCC 25923).
Methods: S. aureus cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 25923) number and stored according to the recommended storage protocols until needed for experiments. Revived and lyophilized state of ATCC strains of S. aureus were …

Clinical And Mechanistic Insights Into Novel Probiotic Functions And Formulations, Jordan Bisanz

Electronic Thesis and Dissertation Repository

Using a combination of hypothesis and discovery based approaches, the goal of this thesis was to better describe novel probiotic functions and their mechanisms while striving to better understand the effect of formulation on Bifidobacterium animalis subsp. lactis, Lactobacillus paracasei and L. rhamnosus.

Using RNA-Seq, a bacterial metatranscriptome analysis of a commonly consumed probiotic yogurt showed that the organisms adapted to storage time and flavor additions. This led to the discovery that in addition to the probiotic health benefits, members of the L. casei group (L. rhamnosus and L. paracasei) produce volatile sulfur compounds mediated by a novel …

Biofield Treatment: An Alternative Approach To Combat Multidrug-Resistant Susceptibility Pattern Of Raoultella Ornithinolytica, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Simulation And Validation Of Radio Frequency Heating Of Shell Eggs, Soon Kiat Lau

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Finite element models were developed with the purpose of finding an optimal radio frequency (RF) heating setup for pasteurizing shell eggs. Material properties of the yolk, albumen, and shell were measured and fitted into equations that were used as inputs for the model. When the egg was heated by itself, heating tend to be focused at the air cell to result in a “coagulation ring.” The focused heating near the air cell of the egg prevented satisfactory pasteurization of the egg, but deeper analysis of the simulation results offered a new perspective on how non-uniform RF heating could occur in …

Spectroscopic Characterization Of Biofield Treated Metronidazole And Tinidazole, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, Snehasis Jana

Mahendra Kumar Trivedi

An Impact Of Biofield Treatment: Antimycobacterial Susceptibility Potential Using Bactec 460/Mgit-Tb System, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Sambhu Charan Mondal, Snehasis Jana

Mahendra Kumar Trivedi

Protective Properties Of Functionalised Tetrazine On An Aerospace Aluminium Alloy (Aa 2024-T3), Swarna Jaiswal, P.C. Rajath Varma, Brendan Duffy, Felicia Mutuma, Patrick Mchale

Articles

Environmental health concerns over conventional chromium based surface treatments on aluminium substrates are well known. Current research efforts have concentrated on developing protective technologies for multiple applications. Such properties would enable manufacturers to address both corrosion and bacterial threats in areas such as fuel tanks and delivery systems.

The present study explores the anticorrosion properties of 1,2-dihydro 1, 2, 4, 5 tetrazine-3, 6-dicarboxylic acid (H₂DCTZ) on a copper rich aerospace aluminium alloy (AA 2024-T3). Furthermore the antimicrobial activity of the tetrazine is evaluated against Gram-positive and Gram-negative bacteria, both capable of inducing corrosion. The protective action of the …

Evaluation Of Biofield Modality On Viral Load Of Hepatitis B And C Viruses, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Study background: Nowadays, hepatitis is a major challenge for clinical research, regulatory bodies, and clinicians who are trying to assess the more effectiveness of antiviral therapy against patients. Viral load count is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of hepatitis. High viral load indicates that the immune system is failed to fight against viruses. The aim of this study was to evaluate the impact of biofield modality on hepatitis B virus (HBV) and hepatitis C virus (HCV) in terms of viral load as surrogate marker.

Method: The …

An Evaluation Of Biofield Treatment On Susceptibility Pattern Of Multidrug Resistant Stenotrophomonas Maltophilia: An Emerging Global Opportunistic Pathogen, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Evaluation Of Biofield Modality On Viral Load Of Hepatitis B And C Viruses, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Sambhu Charan Mondal, Snehasis Jana

Mahendra Kumar Trivedi

Influence Of Biofield Treatment On Physical, Structural And Spectral Properties Of Boron Nitride, Mahendra Kumar Trivedi

Mahendra Kumar Trivedi

Boron nitride (BN) is known for high hardness, thermal stability, thermal conductivity, and catalytic action. The aim of this study was to investigate the effect of biofield treatment on physical, structural and spectral properties of BN powder. The control and treated sample of BN powder were characterized by X-ray diffraction (XRD), surface area analysis and Fourier transform infrared spectroscopy (FT-IR). XRD results indicated that biofield treatment had substantially changed the crystallinity of BN powder as compared to control. Apart from the crystallinity, significant changes were also observed in lattice parameter, density and molecular weight of the treated BN powder as …

Preliminary Evaluation Of Near Infrared Spectroscopy As A Method To Detect Plasma Leakage In Children With Dengue Hemorrhagic Fever, Babs R. Soller, Anon Srikiatkhachorn, Fengmei Zou, Alan L. Rothman, In-Kyu Yoon, Robert V. Gibbons, Siripen Kalayanarooj, Stephen J. Thomas, Sharone Green

Sharone Green

BACKGROUND: Dengue viral infections are prevalent in the tropical and sub-tropical regions of the world, resulting in substantial morbidity and mortality. Clinical manifestations range from a self-limited fever to a potential life-threatening plasma leakage syndrome (dengue hemorrhagic fever). The objective of this study was to assess the utility of near infrared spectroscopy (NIRS) measurements of muscle oxygen saturation (SmO2) as a possible continuous measure to detect plasma leakage in children with dengue.

METHODS: Children ages 6 months to 15 years of age admitted with suspected dengue were enrolled from the dengue ward at Queen Sirikit National Institute for Child Health. …

Determination Of Extracellular Molecules Produced By Vibrio Harveyi Using Ms/Ms, Jose G. Roble

FIU Electronic Theses and Dissertations

Quorum sensing (QS) is a process that allows bacteria to sense the population density of cells around them by communicating with each other via autoinducer molecules. This cross-communication is crucial in the regulation of bacterial processes such as bioluminescence, virulence, and biofilm formation. Previous research by Milburn and Makemson on Vibrio harveyi suggested that in addition of the known biosynthesis of three well-characterized autoinducers, dozens of unknown molecules are also produced and released to the environment by V. harveyi. This study was performed using electrospray tandem mass spectrometry with the purpose of detection and characterization of the extracellular molecules …

Dissolution, Ocean Acidification And Biotic Extinctions Prior To The Cretaceous/Paleogene (K/Pg) Boundary In The Tropical Pacific, Serena Dameron

Masters Theses

The several million years preceding the Cretaceous/Paleogene (K/Pg) boundary has been the focus of many studies. Changes in ocean circulation and sea level, extinctions, and major volcanic events have all been documented for this interval. Important research questions these changes raise include the climate dynamics during the warm, but not hot, time after the decay of the Late Cretaceous greenhouse interval and the stability of ecosystems prior to the mass extinctions at the end-Cretaceous.

I document several biotic perturbations as well as changes in ocean circulation during the Maastrichtian stage of the latest Cretaceous that question whether the biosphere was …

An Effect Of Biofield Treatment On Multidrug-Resistant Burkholderia Cepacia: A Multihost Pathogen, Mahendra Kumar Trivedi, Shrikant Patil, Harish Shettigar, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Novel Neuroprotective Function Of Apical-Basal Polarity Genecrumbs In Amyloid Beta 42 (Aβ42) Mediated Neurodegeneration, Andrew Steffensmeier, Meghana Tare, Oorvashi Roy Puli, Rohan Modi, Jaison Nainaparampil, Madhuri Kango-Singh, Amit Singh

Amit Singh

Alzheimer's disease (AD, OMIM: 104300), a progressive neurodegenerative disorder with no cure to date, is caused by the generation of amyloid-beta-42 (Aβ42) aggregates that trigger neuronal cell death by unknown mechanism(s). We have developed a transgenic Drosophilaeye model where misexpression of human Aβ42 results in AD-like neuropathology in the neural retina. We have identified an apical-basal polarity gene crumbs (crb) as a genetic modifier of Aβ42-mediated-neuropathology. Misexpression of Aβ42 caused upregulation of Crb expression, whereas downregulation of Crb either by RNAi or null allele approach rescued the Aβ42-mediated-neurodegeneration. Co-expression of full length Crb with Aβ42 increased severity of Aβ42-mediated-neurodegeneration, due …

Activation Of Jnk Signaling Mediates Amyloid-Ss- Dependent Cell Death, Meghana Tare, Rohan Modi, Jaison Nainaparampil, Oorvashi Roy Puli, Shimpi Bedi, Pedro Fernandez-Funez, Madhuri Kango-Singh, Amit Singh

Amit Singh

Background: Alzheimer's disease (AD) is an age related progressive neurodegenerative disorder. One of the reasons for Alzheimer's neuropathology is the generation of large aggregates of Aß42 that are toxic in nature and induce oxidative stress, aberrant signaling and many other cellular alterations that trigger neuronal cell death. However, the exact mechanisms leading to cell death are not clearly understood. Methodology/Principal Findings: We employed a Drosophila eye model of AD to study how Aß42 causes cell death. Misexpression of higher levels of Aß42 in the differentiating photoreceptors of fly retina rapidly induced aberrant cellular phenotypes and cell death. We found that …

Novel Neuroprotective Function Of Apical-Basal Polarity Genecrumbs In Amyloid Beta 42 (Aβ42) Mediated Neurodegeneration, Andrew Steffensmeier, Meghana Tare, Oorvashi Roy Puli, Rohan Modi, Jaison Nainaparampil, Madhuri Kango-Singh, Amit Singh

Madhuri Kango-Singh

Alzheimer's disease (AD, OMIM: 104300), a progressive neurodegenerative disorder with no cure to date, is caused by the generation of amyloid-beta-42 (Aβ42) aggregates that trigger neuronal cell death by unknown mechanism(s). We have developed a transgenic Drosophilaeye model where misexpression of human Aβ42 results in AD-like neuropathology in the neural retina. We have identified an apical-basal polarity gene crumbs (crb) as a genetic modifier of Aβ42-mediated-neuropathology. Misexpression of Aβ42 caused upregulation of Crb expression, whereas downregulation of Crb either by RNAi or null allele approach rescued the Aβ42-mediated-neurodegeneration. Co-expression of full length Crb with Aβ42 increased severity of Aβ42-mediated-neurodegeneration, due …