The Effect Of Alcohol On Pfk1 Gene Expression And Feeding Activity In Tetrahymena Thermophila, Evelyn Sowers, Alex Skoulis
Carroll College Student Undergraduate Research Festival
The purpose of this experiment was to test whether alcohol induces starvation conditions in Tetrahymena thermophila. Prior research has shown that exposure to alcohol results in decreases in both the frequency of feeding and overall growth. For this experiment, it was hypothesized that these effects are due to a lack of energy available in the organism for feeding. This hypothesis was tested by monitoring food vacuole formation and expression of the PFK-1 gene in Tetrahymena that were exposed to alcohol. The PFK-1 gene was chosen because its encoded protein plays an essential role in cellular metabolism. For the experiment, control ...
Synthesis And Kinetic Studies Of High-Valent Metal-Oxo Species Generated By Photochemical And Chemical Methods, 2018 Western Kentucky University
Synthesis And Kinetic Studies Of High-Valent Metal-Oxo Species Generated By Photochemical And Chemical Methods, Haiyan Liu
Masters Theses & Specialist Projects
Highly reactive iron-oxo intermediates play important roles as active oxidants in enzymatic and synthetic catalytic oxidation. Many transition metal catalysts are designed for biomimetic studies of the predominant oxidation catalysts in Nature, namely cytochrome P450 enzymes. In this work, a series of iron(IV)-oxo porphyrins [FeIV(Por)O] and manganese(IV)-oxo porphyrins [MnIV(Por)O] have been successfully produced in two electron-deficient ligands by photochemical and chemical methods, and spectroscopically characterized by UV-vis, and 1H-NMR.
With iodobenzene diacetate [PhI(OAc)2] as the oxygen source, iron(III) porphyrin and manganese(III) porphyrin complexes converted to ...
The Bi-Directional Relationship Between Gut Microbiota And Autoimmunity, 2018 Eastern Washington University
The Bi-Directional Relationship Between Gut Microbiota And Autoimmunity, Trevor O. Kirby
EWU Masters Thesis Collection
Humans serve as a major reservoir for a vast number of microbiota. These microbes have evolved symbiotic relationships with humans due to their close proximity with their host. As a result, the immune system adapts to the microbiota thus modulating immunological function. Autoimmunity is a state in which there are aberrant immune responses produced against host tissue. Intestinal bacteria are directly impacted by instances of inflammation brought on by autoimmunity. The complicated nature between autoimmunity and bacterial modulation demonstrates a bi-directional relationship. Here, we utilize experimental autoimmune encephalomyelitis, a model for multiple sclerosis, to explore the bi-directional relationship that disease ...
Identifying Mechanisms Of Host Plant Specialization In Aphis Craccivora And Its Bacterial Symbionts, 2018 University of Kentucky
Identifying Mechanisms Of Host Plant Specialization In Aphis Craccivora And Its Bacterial Symbionts, Thorsten Hansen
Theses and Dissertations--Entomology
Many insects form close relationships with microbial symbionts. Insect symbionts can provide novel phenotypes to their hosts, including influencing dietary breadth. In the polyphagous cowpea aphid, Aphis craccivora, the facultative symbiont Arsenophonus improves aphid performance on one host plant (locust), but decreases performance on other plants. The goal of my thesis was to investigate the mechanism by which Arsenophonus facilitates use of locust. First, I assembled an Aphis craccivora-Arsenophonus-Buchnera reference transcriptome to conduct RNAseq analysis, comparing gene expression in aphids feeding on locust and fava, with and without Arsenophonus infection. Overall, few transcripts were differentially expressed. However, genes ...
Emergence Of New Metabolic Pathways In Escherichia Coli, 2018 University of Colorado, Boulder
Emergence Of New Metabolic Pathways In Escherichia Coli, Michael Kristofich
Undergraduate Honors Theses
Catalytic promiscuity provides a starting point for the evolution of new enzymes. Taking this concept further, a series of promiscuous enzymes may assemble to form the basis of a new metabolic pathway (termed “serendipitous pathway”) that may emerge and become more efficient in adapting bacterial cells that require it to grow. The Copley lab at the University of Colorado at Boulder has been observing genetic changes that improve growth of Escherichia coli cells that lack the essential gene pdxB. These genetic changes are believed to improve the flux of metabolites through one or more serendipitous pathways that make up for ...
Balance And Trade: Charting The Course Of Fungal Symbiosis In Sugarcane Root Systems, 2018 Southern Cross University
Balance And Trade: Charting The Course Of Fungal Symbiosis In Sugarcane Root Systems, Anders Christian Claassens
The root systems of sugarcane (Saccharum L. spp. hybrids) host a variety of symbiotic fungal organisms including arbuscular mycorrhizae (AM) fungi and dark septate endophytes (DSE). AM/DSE symbioses are widely reported to influence the productive capacity of a range of agricultural crops via enhanced nutrient assimilation and plant stress tolerance. Presently, the biological and functional characteristics AM/DSE in sugarcane have only been examined superficially and remain poorly understood. Before the potential benefits of AM/DSE symbioses can be exploited through management strategies, characterisation of the temporal changes in AM/DSE colonisation over the sugarcane crop cycle is required ...
Evidence For The Priming Effect In Single Strain And Simplified Communities Of Estuarine Bacteria, 2017 University of Tennessee, Knoxville
Evidence For The Priming Effect In Single Strain And Simplified Communities Of Estuarine Bacteria, Abigail Amina Edwards
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Through their transformation of naturally occurring organic matter, coastal marine bacteria play an essential role in carbon cycling. A phenomenon termed the priming effect (PE) occurs when microbial communities remineralize recalcitrant organic matter faster in the presence of labile organic matter and may be prevalent in coastal systems. To understand how microbial community members interact to induce PE, it is essential to first understand the mechanisms underlying PE in single strains and simplified bacterial communities. The effect to which different concentrations and sources of labile carbon stimulated the production of bacterial biomass from riverine organic matter by two marine bacteria ...
Functional Analysis Of Microrna Pathway Genes In The Somatic Gonad And Germ Cells During Ovulation In C. Elegans, Carmela Rios
Dissertations (2009 -)
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that play critical roles in animal development and physiology, though functions for most miRNAs remain unknown. Worms with reduced miRNA biogenesis due to loss of Drosha or Pasha/DGCR8 activity are sterile and fail to ovulate, indicating that miRNAs are required for the process of oocyte maturation and ovulation. Starting with this penetrant sterile phenotype and using new strains created to perform tissue-specific RNAi, we characterize the roles of the C. elegans Pasha, pash-1, and two miRNA-specific Argonautes, alg-1 and alg-2, in somatic gonad cells and ...
Characterization Of Neuronal Nicotinic Acetylcholine Receptors And Their Positive Allosteric Modulators, 2017 Brigham Young University
Characterization Of Neuronal Nicotinic Acetylcholine Receptors And Their Positive Allosteric Modulators, Doris Clark Jackson
All Theses and Dissertations
Neuronal nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that are necessary in memory and cognition. They are pentameric and consist of Î± and Î² subunits. They are most commonly heteromeric but, can sometimes be homomeric. nAChRs are activated by many ligands including nicotine (exogenous) and acetylcholine (endogenous).nAChRs are located on hippocampal interneurons. The interneurons, although sparse, control the synchronous firing of the pyramidal cells. However, the hippocampal interneuron structure and function is quite diverse and not fully characterized. Therefore, we sought to quantify nAChR subunit mRNA levels using real-time PCR of CA1 hippocampal interneurons.Surprisingly we found that ...
Elucidating The Interplay Between Sodium Selenite On The Tick Amblyomma Maculatum Selenoprotein Gene Expression, 2017 The University of Southern Mississippi
Elucidating The Interplay Between Sodium Selenite On The Tick Amblyomma Maculatum Selenoprotein Gene Expression, Afnan M. Beauti
Selenium (Se) is an element recognized as an essential micronutrient in eukaryote organisms. Selenoproteins contain selenium as selenocysteine, the 21st amino acid. Selenium plays a role in cell growth and functioning. At low concentrations, it can induce growth and at high concentrations, it can cause a cell to stop growing and potentially have toxic effects on the cell and organism. When selenium levels are high, oxidative stress results by the production of reactive oxidative species. Selenoproteins, however, can aid the antioxidant response in the cell. Ticks are arthropods of interest, as they are one of few that contain many selenogenes ...
Antimicrobial Properties Of S-Benzofuran-2-Yl Ethanol Produced By Biotransformation, 2017 Stephen F Austin State University
Antimicrobial Properties Of S-Benzofuran-2-Yl Ethanol Produced By Biotransformation, Nick Cheatwood, Ann Davis, Elizabeth Schabeck, Kelsi Hooker, Michele Harris
Undergraduate Research Conference
All living organisms contain enzymes to carry out biological reactions, which produce substances with a specific three dimensional shape. Enantiomers are two molecules that are mirror images of each other just as hands are mirror images of each other. It is well known in the pharmaceutical world that one of the enantiomers (one of the mirrorimage molecules) has a positive biological effect while the other can be harmful or have no effect. Currently, any potential pharmaceutical that could exist as enantiomers must have each enantiomer tested for biological activity prior to FDA approval. In our laboratory, we have focused on ...
Bioethics In The Work Of Ernest Everett Just: + Missing - Some 400 Pages, 2017 Southern Methodist University
Bioethics In The Work Of Ernest Everett Just: + Missing - Some 400 Pages, Theodore Walker
Perkins Faculty Research and Special Events
Biology + ethics = bioethics. Here we see that Howard University biologist Ernest Everett Just (born 1883, died 1941) connected biology to ethics.
According to Just, various forms of specific biology (including especially cell biology) plus “general biology” are necessary for explaining adequately the origin of ethical behaviors. Social ethical behaviors, especially mutual aid and cooperative interactions with others and the environment, are essential to evolutionary advances among living creatures, ranging from humans to cells. Accordingly, theory of ethics (moral theory) should have roots in biology.
Also, Just wrote an unpublished book-length manuscript—“some 400 typed pages” (Just 1940)—on seeking the ...
Using Artificial Selection To Understand Orientation Behavior In Drosophila, 2017 Western Oregon University
Using Artificial Selection To Understand Orientation Behavior In Drosophila, Mariah M. Mckechnie, Rachel L. Mendazona, Secilia Torrez, Natalie E. Wallace, Michael J. Baltzley, Kristin L. Latham
Student Theses, Papers and Projects (Biology)
Several studies suggest that the fruit fly Drosophila melanogaster can use magnetic fields for orientation1-4; however, the responses to magnetic fields are not consistent across studies and experiments investigating the mechanism of magnetoreception rely on magnetic fields that are at least 10 times stronger than the magnetic field of the Earth5-6. We are attempting to determine whether Drosophila have the ability to detect Earth-strength magnetic fields by running flies through a progressive Y-maze and then selectively breeding the flies based on their choices in the maze. There are two main hypotheses about the mechanism of magnetoreception in animals. The first ...
Determining Whether Drosophila Melanogaster Have An Innate Directional Preference Based On The Ambient Magnetic Field Of The Earth, Rachel L. Mendazona, Marian Mckechnie, Natalie Wallace, Stephanie Torrez, Michael Baltzley, Kristin Latham
Student Theses, Papers and Projects (Biology)
The status of the fruit fly Drosophila melanogaster as a model organism for behavioral and genetic research makes it an attractive candidate for investigations of the genetic basis of magnetoreception. There are two main hypotheses for how animals detect Earth-strength magnetic fields. One hypothesis is that animals use magnetite, which forms long chains and serves as a magnetic dipole, while the other hypothesis is that animals have a light-dependent magnetic response utilizing cryptochrome. Several studies have found that Drosophila can orient to Earth-strength magnetic fields using a mechanism consistent with a cryptochrome-based magnetoreceptor, but the specifics of the findings have ...
Biomarkers Of Sepsis: A Retrospective Approach, 2017 The University of Akron
Biomarkers Of Sepsis: A Retrospective Approach, Jose M. Roberts
Honors Research Projects
Background: Biomarkers are taking the spotlight in becoming the norm for early diagnoses. Sepsis is an inflammatory disease that increases metabolic rate in children. The first biomarker is hyponatremia. Hyponatremia is a frequent electrolyte imbalance in clinical practice, often observed in children with inflammatory disease and infection. Presence of hyponatremia is associated with electrical signaling imbalances, inflammation and renal dysfunction. The clinical value of hyponatremia in pediatric patients is unknown. The C-reactive protein is a second biomarker. Its presence signifies that necrotic cells and inflammation are present.
Objectives: To evaluate the use of biomarkers in children seen in the Emergency ...
Letter From The Dean, 2017 University of Arkansas, Fayetteville
Letter From The Dean, Lona Robertson
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
No abstract provided.
Effects Of Acute Heat And Oxidative Stress On The Hepatic Expression Of Orexin And Its Related Receptors, 2016 University of Arkansas, Fayetteville
Effects Of Acute Heat And Oxidative Stress On The Hepatic Expression Of Orexin And Its Related Receptors, Stephanie Khaldi
Theses and Dissertations
It is widely known that orexin A and B peptides as well as their receptors are expressed in the hypothalamus and distributed throughout the central nervous system, but there have been few studies regarding its presences in other parts of the body. There is now evidence that orexin (ORX) and its receptors (ORXR1/2) are present in the avian liver; however, their regulation under different environmental conditions is still unknown. In the current study, we sought to determine the effects of heat and oxidative stress using hydrogen peroxide (H2O2) and 4-hydroxynonenal (4-HNE) on the hepatic expression of ORX and ORXR1 ...
External Morphology Of The Eggs Of Asplanchnopus Multiceps (Schrank, 1793) (Rotifera): Solving The 150-Year-Old Case Of Mistaken Identity, 2016 College of Saint Benedict/Saint John's University
External Morphology Of The Eggs Of Asplanchnopus Multiceps (Schrank, 1793) (Rotifera): Solving The 150-Year-Old Case Of Mistaken Identity, Elizabeth S. Wurdak
Biology Faculty Publications
This report calls into question the practice of passing along illustrations and anatomical descriptions from the literature without scrutiny. An error made by Leydig (Zeischrift für wissenschaftliche Zoologie 6:1–120, 1854) in characterizing the egg of Asplanchnopus multiceps was perpetuated in authoritative publications (Hyman, The Invertebrates: Acanthocephala, Aschelminthes, and Entoprocta. The pseudocoelomate Bilateria, McGraw-Hill Book Company, New York, 1951; Voigt, Rotatoria: Die Rädertiere Mitteleuropas, Gebrüder Borntraeger, Berlin-Nikolassee, 1957; Ruttner-Kolisko, Plankton rotifers, biology and taxonomy supplementary edition of Die Binnengewässer 26, Stuttgart, 1974; Koste, Rotatoria: Die Rädertiere Mitteleuropas begründet von Max Voigt. Monogononta 2. Auflage neubearbeitet von Walter Koste, Gebrüder ...
Characterization Of Histone Mutants Associated With Mitotic Defects In Saccharomyces Cerevisiae, 2016 University of Arkansas, Fayetteville
Characterization Of Histone Mutants Associated With Mitotic Defects In Saccharomyces Cerevisiae, Md Riajul Hossain
Theses and Dissertations
Nucleosomes, the basic unit and the building blocks of chromatin have an essential role in the tight packaging of DNA into higher order chromatin. Work from our lab and others have provided information on the contributions of different histone proteins and specific domains within the nucleosome made to create the centromeric chromatin structure required for normal chromosome segregation during mitosis. The DNA entry/exit site is a particular region of the nucleosome where histone H2A, H3 and H4 form critical interactions that appear to be essential for the association of Sgo1, a tension sensing protein that monitors kinetochore-microtubule attachment. In ...
The Thioredoxin Trx-1 Regulates The Major Oxidative Stress Response Transcription Factor, Skn-1, In Caenorhabditis Elegans, 2016 The University of Texas Graduate School of Biomedical Sciences at Houston
The Thioredoxin Trx-1 Regulates The Major Oxidative Stress Response Transcription Factor, Skn-1, In Caenorhabditis Elegans, Katie C. Mccallum
UT GSBS Dissertations and Theses (Open Access)
The ability to respond to hostile environmental conditions is critical for the survival of an organism. Oxidative stress is an adverse state in which reactive oxygen species (ROS) accumulate to a harmful level and, if left unresolved, can lead to cellular dysfunction and organismal disease. Sophisticated detoxification systems, characterized by a battery of enzymatic antioxidants, are utilized to neutralize ROS thereby reducing stress. However, ROS are also purposefully produced by designated cellular enzymes to facilitate the signaling and regulation of critical physiological processes. Therefore, both the production and neutralization of ROS must be tightly controlled. Indeed, the expression of detoxification ...