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Draft Genome Sequence For A Urinary Isolate Of Nosocomiicoccus Ampullae, Evann Elizabeth Hilt, Travis Kyle Price, Katherine Diebel, Catherine Putonti, Alan J. Wolfe 2017 Loyola University Chicago

Draft Genome Sequence For A Urinary Isolate Of Nosocomiicoccus Ampullae, Evann Elizabeth Hilt, Travis Kyle Price, Katherine Diebel, Catherine Putonti, Alan J. Wolfe

Catherine Putonti

A draft genome sequence for a urinary isolate of Nosocomiicoccus ampullae (UMB0853) was investigated. The size of the genome was 1,578,043 bp, with an observed G+C content of 36.1%. Annotation revealed 10 rRNA sequences, 40 tRNA genes, and 1,532 protein-coding sequences. Genome coverage was 727× and consisted of 32 contigs, with an N50 of 109,831 bp.


Draft Genome For A Urinary Isolate Of Lactobacillus Crispatus, Travis Kyle Price, Majed Shaheen, Laurynas Kalesinskas, Kema Malki, Evann Elizabeth Hilt, Catherine Putonti, Alan J. Wolfe 2017 Loyola University Chicago

Draft Genome For A Urinary Isolate Of Lactobacillus Crispatus, Travis Kyle Price, Majed Shaheen, Laurynas Kalesinskas, Kema Malki, Evann Elizabeth Hilt, Catherine Putonti, Alan J. Wolfe

Catherine Putonti

While Lactobacillus crispatus contributes to the stability of normal vaginal microbiota, its role in urinary health remains unclear. As part of an on-going attempt to characterize the female urinary microbiota, we report the genome sequence of an L. crispatus strain isolated from a woman displaying no lower urinary tract symptoms.


Genome Sequences And Annotation Of Two Urinary Isolates Of E. Coli, Travis Kyle Price, Arya Mehtash, Laurynas Kalesinskas, Kema Malki, Evann Elizabeth Hilt, Catherine Putonti, Alan J. Wolfe 2017 Loyola University Chicago

Genome Sequences And Annotation Of Two Urinary Isolates Of E. Coli, Travis Kyle Price, Arya Mehtash, Laurynas Kalesinskas, Kema Malki, Evann Elizabeth Hilt, Catherine Putonti, Alan J. Wolfe

Catherine Putonti

The genus Escherichia includes pathogens and commensals. Bladder infections (cystitis) result most often from colonization of the bladder by uropathogenic E. coli strains. In contrast, a poorly defined condition called asymptomatic bacteriuria results from colonization of the bladder with E. coli strains without symptoms. As part of an on-going attempt to identify and characterize the newly discovered female urinary microbiota, we report the genome sequences and annotation of two urinary isolates of E. coli: one (E78) was isolated from a female patient who self-reported cystitis; the other (E75) was isolated from a female patient who reported that she did not ...


Genomes Of Gardnerella Strains Reveal An Abundance Of Prophages Within The Bladder Microbiome, Kema Malki, Jason W. Shapiro, Travis Kyle Price, Evann Elizabeth Hilt, Krystal Thomas-White, Trina Sircar, Amy B. Rosenfeld, Michael J. Zilliox, Alan J. Wolfe, Catherine Putonti 2017 Loyola University Chicago

Genomes Of Gardnerella Strains Reveal An Abundance Of Prophages Within The Bladder Microbiome, Kema Malki, Jason W. Shapiro, Travis Kyle Price, Evann Elizabeth Hilt, Krystal Thomas-White, Trina Sircar, Amy B. Rosenfeld, Michael J. Zilliox, Alan J. Wolfe, Catherine Putonti

Catherine Putonti

Bacterial surveys of the vaginal and bladder human microbiota have revealed an abundance of many similar bacterial taxa. As the bladder was once thought to be sterile, the complex interactions between microbes within the bladder have yet to be characterized. To initiate this process, we have begun sequencing isolates, including the clinically relevant genus Gardnerella. Herein, we present the genomic sequences of four Gardnerella strains isolated from the bladders of women with symptoms of urgency urinary incontinence; these are the first Gardnerella genomes produced from this niche. Congruent to genomic characterization of Gardnerella isolates from the reproductive tract, isolates from ...


E,E-Farnesol Inhibits Swarming Motility In Burkholderia Cepacia Through Rhamnolipid Production, Stephanie E. Nicholls, Alayna N. Sanderson, Andrea P. Schwartz, Lauren E. Ward, Jessica N. Weisensee, Molly Yandrofski, Tracy L. Collins 2017 Cedarville University

E,E-Farnesol Inhibits Swarming Motility In Burkholderia Cepacia Through Rhamnolipid Production, Stephanie E. Nicholls, Alayna N. Sanderson, Andrea P. Schwartz, Lauren E. Ward, Jessica N. Weisensee, Molly Yandrofski, Tracy L. Collins

Tracy Collins, Ph.D.

Burkholderia cepacia and Candida albicans both exhibit cell-to-cell communication through the use of quorum-sensing molecules (QSM) known as autoinducers. E,E-farnesol is a QSM produced by C. albicans which regulates its conversion from yeast to mycelium. Because there is a positive correlation between the presence of B. cepacia and C. albicans in the lungs of individuals with cystic fibrosis (CF), we examined whether E,E-farnesol had an effect on swarming motility in B. cepacia. Swarming motility was inhibited when B. cepacia was exposed to 250 µM of E,E-farnesol. In addition, there was a 26.8% decrease in rhamnolipid production ...


Bacterial Development Of Resistance To Botanical Antimicrobials, Guillermo Ruiz, Tiffany Turner, Erik Nelson, Linda Sparks, Jeffrey Langland 2017 Southwest College of Naturopathic Medicine

Bacterial Development Of Resistance To Botanical Antimicrobials, Guillermo Ruiz, Tiffany Turner, Erik Nelson, Linda Sparks, Jeffrey Langland

Journal of Evolution and Health

In recent years, increased numbers of multidrug-resistant strains of bacteria have opportunistically and selectively expanded while the pharmaceutical discovery of new antimicrobial therapies has been lacking to combat this growing threat. Like traditional antibiotics, botanicals have historically been used to treat bacterial infections, but it remains unclear if bacteria may have the capability to develop resistance to these therapeutic botanicals. It is believed that one advantage that may prevent or slow resistance to botanical antimicrobials is the presumed presence of the multiple endogenous substances contained within a plant that may act synergistically to inhibit microbial growth. This study examined the ...


Notes On Dairy Bacteriology., L. H. Pammel, I. J. Mead 2017 Iowa State College

Notes On Dairy Bacteriology., L. H. Pammel, I. J. Mead

Bulletin

It is a well known fact that milk varies greatly in quality. Some of it will make butter of the highest quality, some again, owing to injurious organisms, will not make prime butter. The injurious fermentations are much worse at times than at others. Milk as it is received at the creamery, is often “off” in odor. It is, therefore, desirable to know what patron is responsible for this tainted milk. It was supposed by patrons that these odors had their origin in weeds, etc.

Some years ago Mr. Monrad gave, at one of the meetings of the Iowa State ...


Characterization Of Porphyromonas Gingivalis Mfa1 Fimbriae., Jae Yong Lee 2017 University of Louisville

Characterization Of Porphyromonas Gingivalis Mfa1 Fimbriae., Jae Yong Lee

Electronic Theses and Dissertations

Porphyromonas gingivalis, an obligate anaerobic bacterium associated with chronic periodontitis, utilizes various virulence factors to achieve pathogenicity, one of which is the Mfa1 fimbriae. As a surface structure comprising Mfa1 major subunit along with accessory fimbrial proteins Mfa2-5, the Mfa1 fimbriae has been shown to mediate the adherence of P. gingivalis to antecedent bacterial colonizers of the oral cavity to cause increased virulence. However, the spatial relationships amongst the individual subunits and their assembly mechanism have remained unclear. Through immuno-electron microscopy, Mfa1-4 were localized on the surface of P. gingivalis with Mfa1 localizing throughout the fimbriae and Mfa2 in the ...


Bacteriological Results Obtained In Practice With Vat Pasteurization And With One Of The Final Package Methods, B. W. Hammer 2017 Iowa State College

Bacteriological Results Obtained In Practice With Vat Pasteurization And With One Of The Final Package Methods, B. W. Hammer

Bulletin

The pasteurization of milk and cream intended for consumption as such has now been practised for a considerable period. Many sets of figures are available showing the bacterial content before and after pasteurization with various methods and much information has been obtained relative to the bacterial content of pasteurized milk at the time of delivery. The data on the influence of pasteurization on the bacterial content over considerable periods of time are not so numerous, however, and it was with the idea of securing information under practical conditions on this point that the work herein reported was carried out.

The ...


A Bacteriological Study Of The Method Of Pasteurizing And Homogenizing The Ice Cream Mix., B. W. Hammer, L. R. Sanders 2017 Iowa State College

A Bacteriological Study Of The Method Of Pasteurizing And Homogenizing The Ice Cream Mix., B. W. Hammer, L. R. Sanders

Bulletin

In continuing the work being carried out at the Iowa Agricultural Experiment Station on the bacteriology of ice cream, 1, 2, a study has been made of the method of pasteurizing and homogenizing the ice cream mix. This method is at present of some commercial importance and apparently its use is increasing. It enables the factory operators to prepare the mixes at their convenience instead of shortly before freezing and thus seems to offer certain advantages insofar as the operation of the plant is concerned. I t was the object of the work herein reported to study the method from ...


A Bacteriological Study Of The College Creamery Milk Supply, A Case Of Putrid Butter, Purification Of Milk By The Centrifugal Separator, C. H. Eckles, S. E. Barnes 2017 Iowa State College

A Bacteriological Study Of The College Creamery Milk Supply, A Case Of Putrid Butter, Purification Of Milk By The Centrifugal Separator, C. H. Eckles, S. E. Barnes

Bulletin

For several years the Dairy Department has been making experiments in cream ripening and its relation to butter flavor. In the course of this work certain important facts have developed regarding the causes of variation in quality of butter from season to season.

It is a fact understood by all buttermakers that under natural conditions, most of the difficulty experienced in securing the right butter flavor Occurs during the winter season. The annual loss to the dairies and creameries of the state due to this fact is undoubtedly very large.


Some Bacteriological Work In The Dairy, L. H. Pammel 2017 Iowa State College

Some Bacteriological Work In The Dairy, L. H. Pammel

Bulletin

Every wide-awake dairyman takes an interest in the subject of bacteriology as related to the various dairy problems.

Iowa dairymen have taken much interest in this subject owing largely to the fact that dairy papers, dairy columns in agricultural papers have, been discussing this subject in an intelligent manner. Then, too, several American publications, notably Conn1 has given us an excellent summary of the work done in Europe and this country. The amount of original work in this line in the United States is comparatively small, most of it having been done by Prof. Conn in Connecticut We must ...


An Aromatic Bacillus Of Cheese, L. H. Pammel 2017 Iowa State College

An Aromatic Bacillus Of Cheese, L. H. Pammel

Bulletin

During the fall of 1892 my attention was called to a rot of cabbage which was doing serious damage. An isolation of the germs responsible for the disease was undertaken, since none of the higher fungi seemed to be present. Among the germs present was one that had a very striking and peculiar odor when grown in bouillon. It was not unlike that peculiar to Limburger cheese. It resembled old and well-cured cheese. The same germ grown in agar had a pleasant, aromatic and nut-like odor, wholly different than the odor i* bouillon. I suggested to Mr. Wallace, who was ...


Experiments With Mallein, W. B. Niles 2017 Iowa State College

Experiments With Mallein, W. B. Niles

Bulletin

For the benefit of our readers who are not familiar with the agent Mallein and its uses we will say that it is a preparation obtained by growing in culture media, the bacillus of glanders, and that as yet its only use has been as an aid in the diagnosis of doubtful cases of this disease.

The Mallein used in our experiment was prepared by the Bureau of Animal Industry and kindly sent us by request of Dr. Stalker. In regard to the mode of preparation I quote as follows from an article by Drs. Schweinitz and Kilborn: “Acid peptonized ...


Sulfolobus Spindle-Shaped Virus 1 Growth Kinetics, Setarah Mohammad Nader 2017 Portland State University

Sulfolobus Spindle-Shaped Virus 1 Growth Kinetics, Setarah Mohammad Nader

PSU McNair Scholars Online Journal

Geothermal and hypersaline environments are rich in viral particles, among which spindle-shaped morphologies predominate. Currently, viruses with spindle- or lemon-shaped virions are unique to Archaea and belong to two distinct viral families. The larger of the two families, the Fuselloviridae, encompasses spindle-shaped viruses with very short tails, which infect Sulfolobus solfataricus and close relatives. Sulfolobus spindle-shaped virus 1 (SSV1) is the best-known member of the family and was one of the first hyperthermophilic archaeal viruses to be isolated. However, our knowledge of fuselloviral life cycles and the relationships between these viruses and their hosts is still limited. As a result ...


Antibiotic Resistance Modulation And Modes Of Action Of (-)-Α-Pinene In Campylobacter Jejuni, Jasna Kovač, Katarina Šimunović, Zuowei Wu, Anja Klančnik, Franz Bucar, Qijing Zhang, Sonja Smole Možina 2017 University of Ljubljana

Antibiotic Resistance Modulation And Modes Of Action Of (-)-Α-Pinene In Campylobacter Jejuni, Jasna Kovač, Katarina Šimunović, Zuowei Wu, Anja Klančnik, Franz Bucar, Qijing Zhang, Sonja Smole Možina

Qijing Zhang

The aim of the study was to investigate the mode of action of (-)-α-pinene in terms of its modulation of antibiotic resistance in Campylobacter jejuni. Broth microdilution and ethidium bromide accumulation assays were used to evaluate the (-)-α-pinene antimicrobial activity, modulation of antimicrobial resistance, and inhibition of antimicrobial efflux. The target antimicrobial efflux systems were identified using an insertion mutagenesis approach, and C.jejuni adaptation to (-)-α-pinene was evaluated using DNA microarrays. Knock-out mutants of the key up-regulated transcriptional regulators hspR and hrcA were constructed to investigate their roles in C. jejuni adaptation to several stress factors, including osmolytes, and ...


Colonization Density Of The Upper Respiratory Tract As A Predictor Of Pneumonia-Haemophilus Influenzae, Moraxella Catarrhalis, Staphylococcus Aureus, And Pneumocystis Jirovecii., Daniel E Park, Henry C Baggett, Stephen R C Howie, Qiyuan Shi, Nora L Watson, W Abdullah Brooks, PERCH Study Group 2017 George Washington University

Colonization Density Of The Upper Respiratory Tract As A Predictor Of Pneumonia-Haemophilus Influenzae, Moraxella Catarrhalis, Staphylococcus Aureus, And Pneumocystis Jirovecii., Daniel E Park, Henry C Baggett, Stephen R C Howie, Qiyuan Shi, Nora L Watson, W Abdullah Brooks, Perch Study Group

Epidemiology and Biostatistics Faculty Publications

Background.

There is limited information on the association between colonization density of upper respiratory tract colonizers and pathogen-specific pneumonia. We assessed this association for Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus, and Pneumocystis jirovecii. Methods.

In 7 low- and middle-income countries, nasopharyngeal/oropharyngeal swabs from children with severe pneumonia and age-frequency matched community controls were tested using quantitative polymerase chain reaction (PCR). Differences in median colonization density were evaluated using the Wilcoxon rank-sum test. Density cutoffs were determined using receiver operating characteristic curves. Cases with a pathogen identified from lung aspirate culture or PCR, pleural fluid culture or PCR, blood culture ...


Cellular, Particle And Environmental Parameters Influencing Attachment In Surface Waters: A Review, Chunyu Liao, Xiao Liang, Michelle L. Soupir, Laura R. Jarboe 2017 Iowa State University

Cellular, Particle And Environmental Parameters Influencing Attachment In Surface Waters: A Review, Chunyu Liao, Xiao Liang, Michelle L. Soupir, Laura R. Jarboe

Laura R. Jarboe

Effective modelling of the fate and transport of water-borne pathogens is needed to support federally required pollution-reduction plans, for water quality improvement planning, and to protect public health. Lack of understanding of microbial–particle interactions in water bodies has sometimes led to the assumption that bacteria move in surface waters not associated with suspended mineral and organic particles, despite a growing body of evidence suggesting otherwise. Limited information exists regarding the factors driving interactions between micro-organisms and particles in surface waters. This review discusses cellular, particle and environmental factors potentially influencing interactions and in-stream transport. Bacterial attachment in the aquatic ...


Effect Of Aeration Under Pressure On Diacetyl Production In Butter Cultures, C. R. Brewer, C. H. Werkman, M. B. Michaelian, B. W. Hammer 2017 Iowa State College

Effect Of Aeration Under Pressure On Diacetyl Production In Butter Cultures, C. R. Brewer, C. H. Werkman, M. B. Michaelian, B. W. Hammer

Research Bulletin (Iowa Agriculture and Home Economics Experiment Station)

The results presented indicate that the production of diacetyl in butter cultures and pure cultures of citric acid fermenting streptococci can be greatly increased by growing the cultures under 30 pounds or more of air pressure per square inch, provided the cultures are agitated by aeration or stirring. This increase frequently amounts to several hundred percent. In some cases the yield of acetylmethylcarbinol was increased by aeration under pressure, and in other cases it was not. Occasionally, the increase was relatively large.

Pressure without agitation was not effective in increasing the diketone content. The cultures under pressure must be agitaed ...


Reduction Of Acetylmethylcarbinol And Diacetyl To 2,3-Butylene Glycol By The Citric Acid Fermenting Streptococci Of Butter Cultures, B. W. Hammer, G. L. Stahly, C. H. Werkman, M. B. Michaelian 2017 Iowa State College

Reduction Of Acetylmethylcarbinol And Diacetyl To 2,3-Butylene Glycol By The Citric Acid Fermenting Streptococci Of Butter Cultures, B. W. Hammer, G. L. Stahly, C. H. Werkman, M. B. Michaelian

Research Bulletin (Iowa Agriculture and Home Economics Experiment Station)

When acetylmethylcarbinol or diacetyl was added to a tomato bouillon culture of one of the citric acid fermenting streptococci normally present in butter cultures, there was a rapid disappearance of the added reagent and an increase in 2,3-butylene glycol. The amount of the glycol produced accounted, in a general way, for the acetylmethylcarbinol or diacetyl that disappeared. The added reagent did not usually disappear completely. In trials with acetylmethylcarbinol the change of the carbinol to the glycol was delayed when sulfuric acid was added in amounts to yield a pH of from 3.8 to 4.0.

There was ...


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