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About Logan Weihe And Beloved Microcosm, Logan M. Weihe 2017 University of Illinois at Chicago

About Logan Weihe And Beloved Microcosm, Logan M. Weihe

Steeplechase

No abstract provided.


Mixed Municipal Solid Waste Hygienisation For Refuse-Derived Fuel Production By Ozonation In The Novel Configuration Using Fluidized Bed And Horizontal Reactor, Witold Zukowski 2017 Cracow University of Technology

Mixed Municipal Solid Waste Hygienisation For Refuse-Derived Fuel Production By Ozonation In The Novel Configuration Using Fluidized Bed And Horizontal Reactor, Witold Zukowski

Witold Zukowski

Purpose
This study aimed to determine whether mixed municipal solid waste used for alternative fuel production can be effectively treated with ozone hygienisation in a gaseous phase.
Methods
It was determined that the use of a fluidised bed reactor and a horizontal reactor with respect to processing the light fraction and the heavy fraction of waste effectively contributes to the elimination or reduction of the number of selected groups of microorganisms inhabiting mixed municipal solid waste. With the use of microbiological analysis, the presence of vegetative bacteria, spores, fungi, actinomycetes and pathogenic bacteria, i.g. Staphylococcus spp., Escherichia coli, Salmonella ...


Anti-Staphylococcal Activity Of Variovorax Paradoxus Eps, Patricia Holt-Torres 2017 California State University, San Bernardino

Anti-Staphylococcal Activity Of Variovorax Paradoxus Eps, Patricia Holt-Torres

Electronic Theses, Projects, and Dissertations

Variovorax paradoxus EPS is a gram-negative rod isolated from the sunflower rhizosphere at CSUSB. Preliminary research has shown that Variovorax paradoxus EPS has anti-staphylococcal activity in liquid and solid co-culture. Anti-staphylococcal activity of Wild type and V. paradoxus EPS 𝚫4519 on 0.5% YE agar with embedded S. aureus AH1710 supports the idea that a soluble molecule is responsible for this activity, as the agar acted as a physical barrier between V. paradoxus EPS and S. aureus colonies. Preliminary genetic analysis of V. paradoxus EPS identified three loci that suitable candidates for the synthesis of a potential anti-staphylococcal small ...


An Integrated Bioinformatic/Experimental Approach For Discovering Novel Type Ii Polyketides Encoded In Actinobacterial Genomes, Wubin Gao 2017 University of New Mexico

An Integrated Bioinformatic/Experimental Approach For Discovering Novel Type Ii Polyketides Encoded In Actinobacterial Genomes, Wubin Gao

Chemistry ETDs

Discovery of new natural products (NPs) is critical both for diseases treatment and crops protection. Numerous NP biosynthetic gene clusters (BGCs) in sequenced microbial genomes allow identification of new NPs through genome mining. Developing an integrated bioinformatic/experimental approach for discovering novel type II polyketides (PK-IIs) facilitates investigation of this family of NPs in an efficient, systematic way. Here, we developed an approach to analyze ketosynthase α/β (KSα/β) gene sequences to predict PK-II core structures, allowing us to target novel PK-II BGCs either from isolated genomic DNA or genomes from the NCBI databank, and to isolate novel PK-IIs ...


Scanning Labyrinthulomycete Genomes For Yeast Transcription Factor Binding Site Motifs, Jackie L. Collier, Joshua Rest 2017 SUNY Stony Brook

Scanning Labyrinthulomycete Genomes For Yeast Transcription Factor Binding Site Motifs, Jackie L. Collier, Joshua Rest

Interdisciplinary Research Data

To develop broadly useful methods for the genetic manipulation of Labyrinthulomycetes (a diverse group of ubiquitous osmoheterotrophic marine protists), it is essential to understand the similarities and differences in regulation of gene expression among them. Toward this end we have used FIMO from the MEME suite (http://meme-suite.org/doc/fimo.html) to identify potential transcription factor binding sites in each of the three available genome sequences: Aplanochytrium kerguelense PBS07, Schizochytrium aggregatum ATCC 28209, and Aurantiochytrium limacinum ATCC MYA-1381


Investigation Of Media Effects On Sinorhizobium Meliloti Glucose Minus, Roxana Apostol 2017 University of Redlands

Investigation Of Media Effects On Sinorhizobium Meliloti Glucose Minus, Roxana Apostol

Undergraduate Honors Theses

Although nitrogen fertilizers have certain benefits, the over application of such compounds often results in damages to the ecosystem. In this project, we focus our study on Sinorhizobiom meliloti, a species that lives in symbiosis with alfalfa plants, and through its nitrogen fixation capabilities, restores nitrogen levels in the soil. In our study we aim to gain a better understanding of the carbon metabolism in S. meliloti, mainly by looking at growth patterns in the presence of different carbon sources. Our research picked up on Erik Arvey’s discoveries which pointed out that sucrose inhibits growth of certain glucose minus ...


Toward The History Of Study Of Symbiogenesis: On The English Translation Of B. M. Kozo-Polyansky’S A New Principle Of Biology (1924), Victor Fet 2016 Marshall University

Toward The History Of Study Of Symbiogenesis: On The English Translation Of B. M. Kozo-Polyansky’S A New Principle Of Biology (1924), Victor Fet

Victor Fet

We reproduce the text by Victor Fet, which was read on 6 October 2011 at the Moscow Society of Naturalists during the presentation of new book translation (B.M. Kozo- Polyansky. Symbiogenesis: A New Principle of Evolution / transl. by Victor Fet; ed. by Victor Fet & Lynn Margulis. Cambridge, Mass.: Harvard University Press, 2010. 138 p.) This half- forgotten book by Boris M. Kozo-Polyansky was known only by name to Western biologists. Victor Fet gives a brief history of this new translation, enthusiastically initiated and supported by Lynn Margulis (1938–2011), a famous naturalist who was always eager to gave credit where credit was due. Kozo- Polyansky, along with Merezhkovsky, Portier, and Wallin, pioneered symbiogenetic ideas that were brilliantly developed and vindicated starting from 1960–1970s. It was Lynn Margulis who noticed also that Kozo- Polyansky preceded E. Chatton in recognizing the profound diff erence between prokaryotes and eukaryotes; in fact, he maintained that this difference was due to symbiogenetic, complex nature of the eukaryotic cell! Two “scientistic” poems (in Russian) by Victor Fet are included, dedicated to all the prophets and martyrs of science.


Jmh Dissertation 2016.Pdf, Jennifer Hayashi 2016 University of Massachusetts - Amherst

Jmh Dissertation 2016.Pdf, Jennifer Hayashi

Jennifer Hayashi

Mycobacterium is a diverse genus of actinobacteria that includes the causative agents of human tuberculosis and leprosy. Mycobacteria are protected by their unique, multilaminar cell envelope, which grants them intrinsic resistance to environmental challenges such as antibiotics. This essential cellular structure is elongated at the polar ends of cells, but the regulation of cytosolic precursor synthesis and localized envelope synthesis remains unclear. Here, we present the PMf (plasma membrane free of cell wall components), a membrane domain distinct from the bulk plasma membrane of Mycobacterium smegmatis. Proteomic and lipidomic characterization demonstrate that the PMf contains lipidic substrates ...


The Elasmobranch-Microbe Relationship: Trimethylamine N-Oxide Synthesis, Urea Hydrolysis, And Microbe-Osmolyte Interactions In The Atlantic Stingray, Dasyatis Sabina, Kaitlin Kelly Doucette 2016 The University of Southern Mississippi

The Elasmobranch-Microbe Relationship: Trimethylamine N-Oxide Synthesis, Urea Hydrolysis, And Microbe-Osmolyte Interactions In The Atlantic Stingray, Dasyatis Sabina, Kaitlin Kelly Doucette

Master's Theses

The elasmobranch osmoregulatory strategy is predicated on the accumulation of nitrogenous compounds, primarily urea and trimethylamine n¬-oxide (TMAO). Despite the abundance of these plasma osmolytes, it is notable that elasmobranchs appear to lack urease and TMA oxidase (Tmase), enzymes that scavenge urea-nitrogen and synthesize TMAO, respectively. However, urease and Tmase are found in many species of bacteria. Therefore, I hypothesized that intestinal bacteria are responsible for urease and Tmase activity in elasmobranchs. Absent dietary nitrogen sources, I evaluated the effects of reduced intestinal microbiota on osmoregulation in Atlantic stingray (Dasyatis sabina) in vivo. D. sabina were given daily broad-spectrum ...


Fecal Bacterial Communities As An Indicator Of Trophic Interactions Among Anuran Larvae, Steven Jacob Everman 2016 University of Southern Mississippi

Fecal Bacterial Communities As An Indicator Of Trophic Interactions Among Anuran Larvae, Steven Jacob Everman

Master's Theses

Anurans are mass spawners, often with multiple females spawning together, resulting in thousands of tadpoles sharing a habitat. Such large numbers of tadpoles with limited dispersal can lead to intense competition for resources. Inter and intra-specific competition for food could have negative impacts on the growth and survival of smaller tadpoles. Fecal bacterial communities have the potential to be used as indicators of changes in diet making it possible to determine if tadpoles in the wild are eating the same food or not. After feeding on two prepared diets that differed in the percentage of complex carbohydrates, the fecal bacterial ...


Identification Of Microorganisms Isolated From Mars-Bound Spacecraft Using Maldi-Tof, Melanie K. Phillips 2016 Cal Poly, San Luis Obispo

Identification Of Microorganisms Isolated From Mars-Bound Spacecraft Using Maldi-Tof, Melanie K. Phillips

STAR (STEM Teacher and Researcher) Presentations

The primary goal of the Biotechnology and Planetary Protection Group (BPPG) at the Jet Propulsion Laboratory (JPL) is to prevent microbial contamination between Earth and other planetary bodies. This helps preserve the ability to study other worlds as they exist in their natural states and protects our biosphere from extraterrestrial life. The process for identifying microbes located on space-bound hardware in JPL can be more efficient and precise by using the technique called matrix-assisted laser desorption and ionization time of flight (MALDI-TOF). Once this identification technique is fully implemented, the process time will be reduced from two weeks to one ...


Improving Metabolic Engineering And Characterization Of Clostridium Thermocellum For Improved Cellulosic Ethanol Production, Beth Alexandra Papanek 2016 University of Tennessee, Knoxville

Improving Metabolic Engineering And Characterization Of Clostridium Thermocellum For Improved Cellulosic Ethanol Production, Beth Alexandra Papanek

Doctoral Dissertations

Biofules are an important option for humanity to move away from its dependence on fossil fuels. Transitioning from food crops to lignocellulosic alternatives for the production of biofuels is equally important. Most commonly, biofuels are produced using a crop such as corn or soybeans to feed sugars to the yeast, Saccharomyces cerevisiae for the fermentation of ethanol. Lignocellulosic biofuel production would eliminate the need for food crops and transition to biomass such as switchgrass, poplar, or corn stover. Currently, lignocellulosic biofuel production is limited primarily because of the cost of converting the biomass to fermentable sugars than can then be ...


Chemokines And Peptides That Promote And Inhibit Cmv Entry, Elisabeth Anne Pitt 2016 University of Tennessee, Knoxville

Chemokines And Peptides That Promote And Inhibit Cmv Entry, Elisabeth Anne Pitt

Masters Theses

Human cytomegalovirus (HCMV) causes morbidity and mortality in congenitally infected newborns, transplant recipients, and AIDS patients. Currently, there is no approved CMV vaccine to address these issues. In an effort to develop an alternative treatment to CMV we tested our hypothesis that heparan sulfate binding D-peptides would be effective against multiple HCMV strains in vitro and it would be effective in vivo against murine CMV (MCMV) (Chapter 1). We show that the D-peptide is able to reduce CMV infection in vitro and in vivo. Another approach to combating CMV infections is to neutralize pathogenic factors that contribute to CMV spread ...


Relationship Between Morphogenesis And Secretion In The Filamentous Fungus Aspergillus Nidulans, Lakshmi Preethi Yerra 2016 University of Nebraska-Lincoln

Relationship Between Morphogenesis And Secretion In The Filamentous Fungus Aspergillus Nidulans, Lakshmi Preethi Yerra

Dissertations and Theses in Biological Sciences

Filamentous fungi have a long history in biotechnology for the production of food ingredients, pharmaceuticals and enzymes. The advancements made in recent years have earned filamentous fungi such as Aspergillus species a dominant place among microbial cell factories. Although the model fungus A. nidulans has been extensively studied, the genetic and regulatory networks that underlie morphogenesis and development have yet to be fully characterized. The Rho GTPases (Cdc42 and RacA) are one of the most important regulators of the morphogenetic processes among diverse eukaryotic organisms. Although the functions of these GTPases are relatively well-characterized, little is known about their downstream ...


Engineering A Mutation In The Heparin Binding Pocket Of The Human Fibroblast Growth Factor, Roshni Patel 2016 University of Arkansas, Fayetteville

Engineering A Mutation In The Heparin Binding Pocket Of The Human Fibroblast Growth Factor, Roshni Patel

Chemistry & Biochemistry Undergraduate Honors Theses

Fibroblast growth factors (FGFs) are family of proteins that belong to a group of growth factors that are found in mammals and play an important role in angiogenesis, differentiation, organogenesis, and tissue repair. In summary, their main functionality is involved in cell division and proliferation. Because FGFs plays such a vital role in cell proliferation, they are mainly involved in the process of wound healing and injuries. FGF binds to its ligand, heparin—a heavily sulfated glycosaminoglycan. The binding of heparin to FGF occurs through electrostatic interactions, specifically between the negatively charged sulfate groups on heparin and positively charged residues ...


Genomic Diversity Of Pseudoalteromonas Atlantica From Geographically Distant Deep Marine Basins, Erika N. Youngquist 2016 The University of Tennessee Knoxville

Genomic Diversity Of Pseudoalteromonas Atlantica From Geographically Distant Deep Marine Basins, Erika N. Youngquist

University of Tennessee Honors Thesis Projects

No abstract provided.


Effects Of Lactobacillus Rhamnosus Milk Isolate On The Production Of Inflammatory Cytokines In Enterocytes, Beverly C. Ngeny 2016 East Tennessee State Universtiy

Effects Of Lactobacillus Rhamnosus Milk Isolate On The Production Of Inflammatory Cytokines In Enterocytes, Beverly C. Ngeny

Electronic Theses and Dissertations

In the gastrointestinal tract, probiotics have been shown to promote host immunity and to regulate immune signaling pathways. This study used Caco-2 cell line to examine the effects of a Lactobacillus rhamnosus isolate from “amabere amaruranu” a Kenyan traditional cultured milk, on the production inflammatory cytokines in enterocytes. Live Lactobacillus rhamnosus (MRS6AN), its cytoplasmic fraction (CF), filtered spent broth (FSB) or heat inactivated FSB (HIB) were used as treatments on differentiated Caco-2 cell monolayer in transwells. Cytokine content in the cell lysates, apical and basolateral supernatants were determined using ELISA. Caco-2 cell lysate treatments showed significantly increased anti-inflammatory TGF-β (ng ...


Oxidative Stress Response In Archaea: Elucidation Of Oxidant Sensing And Tolerance Mechanisms In Methanosarcina Acetivorans, Matthew Edward Jennings 2016 University of Arkansas, Fayetteville

Oxidative Stress Response In Archaea: Elucidation Of Oxidant Sensing And Tolerance Mechanisms In Methanosarcina Acetivorans, Matthew Edward Jennings

Theses and Dissertations

Methanogens are archaea possessing a conserved metabolic pathway which produces methane. Many of the enzymes in the methanogenesis pathway are Fe-S proteins, meaning methanogens are sensitive to conditions which disrupt Fe-S clusters. Molecular oxygen is capable of disrupting Fe-S clusters through oxidation of the iron atoms. Furthermore, reduced iron can facilitate the production of reactive oxygen species (ROS), meaning methanogens must possess antioxidant mechanisms. Detection and eradication of ROS is important for all cells, due to the potentially fatal consequences of unchecked oxidation. This dissertation presents two separate projects investigating mechanisms the model methanogen Methanosarcina acetivorans possess for dealing with ...


The Prevalence Of Escherichia Coli And Fecal Coliforms On Backpacks Of College Students In Central Kentucky, Samantha McMichael 2016 Midway College

The Prevalence Of Escherichia Coli And Fecal Coliforms On Backpacks Of College Students In Central Kentucky, Samantha Mcmichael

Butler Journal of Undergraduate Research

Abstract

Escherichia coli (E. coli) is a gram negative, rod-shaped, facultative anaerobic bacterium, it is normally found in the lower intestines of warm-blooded animals and humans. E. coli and/or fecal coliforms have been used as fecal indicators in water, food, and contact surfaces. The purpose of this experiment is to identify the prevalence of E. coli and fecal coliforms on the bottom of backpacks belonging to college students in central Kentucky. Forty samples were taken from bottoms of college students’ backpacks in central Kentucky. Sterile swabs were used to collect the samples from the backpacks. The swabs were placed ...


No Nonsense: The Protection Of Wild-Type Mrnas From Nonsense-Mediated Mrna Decay In Saccharomyces Cerevisiae, Krista Patefield 2016 University of Nebraska-Lincoln

No Nonsense: The Protection Of Wild-Type Mrnas From Nonsense-Mediated Mrna Decay In Saccharomyces Cerevisiae, Krista Patefield

Dissertations and Theses in Biological Sciences

Gene regulation in eukaryotes is tightly controlled at multiple levels to ensure proper expression and cellular homeostasis. Misregulation of gene expression is a common source of genetic disease. One mechanism by which cells are able to control gene expression is through the synthesis and degradation of the mRNA molecules encoding the genes. The transcription and degradation of mRNA molecules controls the pool mRNAs that are available to the translational machinery. One of the well-studied mRNA decay pathways is the Nonsense-Mediated mRNA Decay pathway (NMD). Originally, NMD was discovered as a posttranscriptional mRNA surveillance mechanism responsible for the deadenylation-independent decapping and ...


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