Environmental Microbiology and Microbial Ecology Commons^™

Considerations For The Use Of Mycorrhizal Fungi Inoculants In Coal Mine Reclamation And Reforestation In Appalachia: A Guide Of Best Practices And Management Recommendations, Cole Hansen Reistrup

Master's Projects and Capstones

Nearly all terrestrial plants form symbiotic relationships with mycorrhizal fungi. These beneficial soil microbes support plant health by increasing plants’ access to nutrients and water, protecting them from pathogens, improving soil properties, and more. A challenge to restoring forests on coal mined land in Appalachia is that mine soils are often degraded in a number of ways, including lacking mycorrhizal fungi, which makes mine soils difficult for trees to grow upon. Substantial improvements to coal mine reforestation success have been made since the development of the Forestry Reclamation Approach in 2005 that provides guidelines for how to create a suitable …

The Influence Of Sampling Frequency On Bee Species Richness, Courtney Caccamo

Biology Student Scholarship

As one of Earth’s most valuable pollinators, bees provide important pollination services to wild plant species and crops alike (Winfree 2010). But, in recent years, bee populations appear to be declining due to a variety of anthropogenic drivers (Potts et al. 2010). Quantifying the extent of bee population decline is difficult because there is currently a lack of a standardized protocol for how best to survey bee populations (Tepedino & Portman 2021). One of the commonly used survey methods is pan trapping, which involves leaving out yellow, white, and blue bowls filled with soapy water to passively sample the species …

Exploring The Dynamics Of Bacterial Populations In The Gut Microbiota, Shaikh Obaidullah

Biology and Medicine Through Mathematics Conference

No abstract provided.

The Effect Of Algal Wastewater Treatment Systems On Microbial Communities, Alexis Leach

University Honors Program Senior Projects

In collaboration with the Metropolitan Water Reclamation District of Greater Chicago and Gross-Wen Technologies, I examined the microbial communities in experimental tertiary wastewater treatment systems employing algal phytoremediation. Three algal phytoremediation systems were examined: two vertical conveyor belt systems, known as the 10-foot belt system and the series 4 system, and a third system lacking a converter belt in which planktonic algae grew in a control pond. I used EcoPlates to measure carbon substrate utilization by microorganisms in each system. EcoPlates contain thirty-one different carbon substrates, with a dye that indicates microbial use of each substrate. I conducted a Principal …

The Use Of Aloe-Based Silver Nanoparticles As A Possible Treatment For Contaminated Water, Lozetta Davis

Scholar Week 2016 - present

Water contamination is a world-wide issue that causes the death of millions yearly. This comes from regularly tainted water with water borne pathogens, which is typically seen in undeveloped areas. As antibiotics are used more, more resistance is being seen, and thus newer methods of microbial contamination need to be developed. Silver nanoparticles have shown success in microbial contamination on petri dishes, but not in a water system. It was hypothesized that if silver nanoparticles were added to bacteria contaminated water they would be able to reduce the amount of bacteria present. Silver nanoparticles that are synthesized from plants such …

Molecular Biodiversity Of Foraminifera, Rabindra Thakur

Masters Theses

Foraminifera are a diverse clade of mostly shell-building single-celled organisms. Estimation of foraminiferal diversity is critical for understanding past and present climatic conditions, as they are highly sensitive to environmental perturbations. Biodiversity estimates of foraminifera began with the counting of test (i.e., shell) microfossils composed of calcium carbonate, as they are well preserved in sediment samples. However, this view has changed with molecular biodiversity estimates, which suggest that early-diverging single-chamber (i.e., "monothalamid") species that lack preservation ability are more diverse than anticipated. Although biodiversity estimates of foraminifera at the molecular level have changed our perceptions, they possess various challenges, especially …

The Application Of Microbial Source Tracking To Aid In Site Prioritization For Remediation In Lower Michigan, John J. Hart

Masters Theses

Non-point source fecal pollution is a threat to both the environment and public health. Climate change, aging infrastructure, and intensified agricultural practices are predicted to accentuate this issue. In Michigan, due to the high instance of aging infrastructure and intensified agriculture, non-point source fecal pollution has caused many waterbodies to exceed the state standards posing a risk to recreational activities and source water. Due to this threat, there is an increased effort to identify and remediate these sources. My study focused on improving the identification of non-point source fecal pollution through a combination of culture-based and molecular fecal indicator bacteria …

Enrichment Media Selection And Co-Culture Potential Among Exoelectrogen Bacteria Vary With Ecological Factors, Nur Syafira Khoirunnisa, Syaiful Anwar, Untung Sudadi, Dwi Andreas Santosa

Makara Journal of Science

Staphylococcus saprophyticus ICBB 9554 and Citrobacter freundii ICBB 9763 are exoelectrogen bacteria applied as microbial fuel cells (MFC). We selected enrichment media for both these exoelectrogen bacteria, characterized their synergistic traits, and evaluated the growth conditions under different ecological factors. In this study, different enrichment media, such as those containing sugar, molasses, and palm sugar (2, 4, and 6% w/v) were tested for S. saprophyticus ICBB 9554. Meanwhile, technical sodium acetate (NaAc), commercial vinegar 25%, and cider vinegar (5, 10, and 15 mM acetate concentration) were tested for C. freundii ICBB 9763. Both the exoelectrogen bacteria were tested for the …

Fish Farming Wastewater Treatment Using Algae-Cyanobacterial Inocula, Yolanys Aranda

Graduate Industrial Research Symposium

Fish is one of the most important nutrient sources of the human diet, and its low-cost aquaculture production is advantageous. During processing, large quantities of organic and inorganic nutrients are produced. Discharging fish farming wastewater to the environment without proper treatment can support harmful algal growth. Therefore, aquaculture effluent must be treated prior to discharge to the receiving waters. In this study, six different cyanobacteria and algae species, including Chlorococcumminutum, Porphyridiumcruentum, Chlorella vulgaris, Microcystis aeruginosa, Chlamydomonas reinhardtii, and Fischerellamuscicola were studiedto treatfish aquaculture wastewater, using mix-culture bacteria as the inoccolum. All samples were incubated for 21 days. Parameters such as …

Susceptibility Of Pre-Adult Biological Stages Of Mythimna Separata (Walker) (Lepidoptera: Noctuidae) To Three Entomopathogenic Fungi (Hypocreales), Melissa P. Montecalvo, Marcela M. Navasero

The Philippine Agricultural Scientist

Armyworms are polyphagous insect pests of agricultural crops. Their population is usually managed below threshold level using insecticides. However, a single control measure is insufficient to attain a sustainable pest management. Hence, this research was conducted to assess the three species of entomopathogenic fungi as potential biological control agents against Mythimna separata (Walker) or paddy armyworm. Dose mortality assays were conducted to determine the pathogenicity of Beauveria bassiana (Bals.) Vuill., Metarhizium anisopliae (Metschn.) Sorokin, and Metarhizium rileyi (Farlow) Kepler, S.A. Rehner and Humber against various biological stages of M. separata including egg, 1^st to 6^th larval instars, prepupa, …

Assessing Functional Biodiversity For The Future Of Plants, Planet, And People, Ali Loker

Doctoral Documents from Doctor of Plant Health Program

Biodiversity plays a critical role in supporting life in global ecosystems and its links to ecosystem services and sustainability are recognized by scientific and non-scientific communities. Growing awareness of the importance of biodiversity is accelerated by discussions of its loss, and how to design interventions to conserve and mitigate a biodiversity crisis. Stakeholders are funding and implementing assessment strategies at various scales to help direct conservation efforts. There is also growing interest in measuring and communicating biodiversity outcomes.

Functional biodiversity characterizes the multiplicity of life forms into groups based on their diverse contributions to natural and agro-ecosystems. Assessing functional biodiversity …

Evaluation Of Qpcr To Detect Shifts In Population Composition Of The Rhizobial Symbiont Mesorhizobium Japonicum During Serial In Planta Transfers, Kenjiro W. Quides, Yoobeen Lee, Teresa Hur, Hagop S. Atamian

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Microbial symbionts range from mutualistic to commensal to antagonistic. While these roles are distinct in their outcome, they are also fluid in a changing environment. Here, we used the Lotus japonicus–Mesorhizobium japonicum symbiosis to investigate short-term and long-term shifts in population abundance using an effective, fast, and low-cost tracking methodology for M. japonicum. We use quantitative polymerase chain reaction (qPCR) to track previously generated signature-tagged M. japonicum mutants targeting the Tn5 transposon insertion and the flanking gene. We used a highly beneficial wild type and moderately beneficial and non-beneficial mutants of M. japonicum sp. nov. to demonstrate the specificity …

Under Ice Photosynthetic Primary Production And Dark Carbon Fixation In A Temperate Freshwater System, Vanessa Cubillos Tellez

Dissertations, Master's Theses and Master's Reports

Ice-covered lakes are vulnerable to environmental change, especially those in the Northern Hemisphere where ice cover is rapidly declining due to global warming. These changes can alter metabolic processes and disrupt carbon cycling driven by primary producers who form the base of the food chain and are key to sustaining ecosystem function. Photosynthetic primary production and dark carbon fixation under the ice in the Keweenaw Waterway, a temperate freshwater system that is ice-covered for ~3 months out of the year, were studied using a carbon isotopic labeling (14C-bicarbonate) technique. Water samples were collected weekly during ice cover and monthly during …

Nitrite Cycling In The Primary Nitrite Maxima Of The Eastern Tropical North Pacific, Nicole M. Travis, Colette L. Kelly, Margaret R. Mulholland, Karen L. Casciotti

OES Faculty Publications

The primary nitrite maximum (PNM) is a ubiquitous feature of the upper ocean, where nitrite accumulates in a sharp peak at the base of the euphotic zone. This feature is situated where many chemical and hydrographic properties have strong gradients and the activities of several microbial processes overlap. Near the PNM, four major microbial processes are active in nitrite cycling: ammonia oxidation, nitrite oxidation, nitrate reduction and nitrite uptake. The first two processes are mediated by the nitrifying archaeal/bacterial community, while the second two processes are primarily conducted by phytoplankton. The overlapping spatial habitats and substrate requirements for these microbes …

The Impact Of Nutrient Depletion On The Effect Of Hhq On Emiliania Huxleyi, Marley Gonsalves

Honors Theses and Capstones

Emiliania huxleyi is a ecologically important, bloom-forming coccolithophore. HHQ is a bacteria compound that causes cell stasis in E. huxleyi that can lead to bloom termination. This study analyzed the role that nutrient depletion plays in this relationship. Results show that nitrogen depletion protects cells from the effects of HHQ, affecting bloom dynamics.

Extension Of The Ergot Alkaloid Gene Cluster, Samantha Joy Fabian

Graduate Theses, Dissertations, and Problem Reports

Specialized metabolites produced by fungi impact human health. A large portion of the pharmaceuticals currently on the market are derived from metabolites biosynthesized by microbes. Ergot alkaloids are a class of fungal metabolites that are important in the interactions of environmental fungi with insects and mammals and also are used in the production of pharmaceuticals. In animals, ergot alkaloids can act as partial agonists or antagonists at receptors for 5-hydroxytryptamine (serotonin), dopamine, and noradrenaline as ergot alkaloids have chemical structures similar to those neurotransmitters. Therefore, they affect insects and mammals that consume them and can be used to produce drugs …

Rapid Bacterial And Fungal Successional Dynamics In First Year After Chaparral Wildfire, M. Fabiola Pulido-Chavez, James W. J. Randolph, Cassandra A. Zalman, Loralee Larios, Peter M. Homyak, Sydney I. Glassman

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The rise in wildfire frequency and severity across the globe has increased interest in secondary succession. However, despite the role of soil microbial communities in controlling biogeochemical cycling and their role in the regeneration of post-fire vegetation, the lack of measurements immediately post-fire and at high temporal resolution has limited understanding of microbial secondary succession. To fill this knowledge gap, we sampled soils at 17, 25, 34, 67, 95, 131, 187, 286, and 376 days after a southern California wildfire in fire-adapted chaparral shrublands. We assessed bacterial and fungal biomass with qPCR of 16S and 18S and richness and composition …

Guano Among Bat Species From Two Regions Shows Influence Of Geography And Diet On Bacterial Community, Rahma Ahmed, Thomas Mcelroy, Shannon Whitney, Lydia Moore

Symposium of Student Scholars

Studies of bat guano have shown that the diversity and structure of associated microbial communities can be related to factors such as host phylogeny, life history and reproductive stage, geography, and diet. Many insectivorous bat species in the southeastern U.S. have generalist diets that may shift seasonally to take advantage of abundant prey species or maximize caloric intake. Seasonal shifts in prey availability or consumption should be reflected in a guano microbiome change. We also expected to detect distinct guano microbiomes within species. Within species, distinct microbial communities related to geography, and finally life history and reproductive stage. We compared …

Identification Of The Type Eleven Secretion System (T11ss) And Characterization Of T11ss-Dependent Effector Proteins, Alex S. Grossman

Doctoral Dissertations

Host-associated microbes live in dangerous environments as a result of host immune killing, nutrient provisioning, and physiological conditions. Bacteria have evolved a host of surface and secreted proteins to help interact with this host environment and overcome nutrient limitation. The studies included within this dissertation describe the identification of a novel bacterial secretion system which has evolved to transport these symbiosis mediating proteins. This system, termed the type eleven secretion system (T11SS), is present throughout the Gram negative phylum Proteobacteria, including many human pathogens such as Neisseria meningitidis, Acinetobacter baumanii, Haemophilus haemolyticus, and Proteus vulgaris. Furthermore, …

Characterizing The Cutaneous Microbiome Of Eurycea Lucifuga As A Potential Defense Against Chytridiomycosis, Madeline Key

Senior Honors Theses

Chytridiomycosis is an emerging infectious disease that is significantly reducing global amphibian populations. The disease is caused by Batrachochytrium dendrobatidis (Bd), a fungus that lethally modifies amphibian skin. Recent research has suggested that the cutaneous microbiome of individual amphibians may play a role in susceptibility to the pathogen. In this study, twelve cave salamanders (Eurycea lucifuga) were collected. Cutaneous bacteria from each salamander were isolated and identified using Sanger Sequencing. Additionally, a Bd-challenge assay was performed to determine each isolate’s antifungal activity. Results indicated many microbial isolates possessed inhibitory capabilities against Bd, which may …