Functions Of Ecosystems: Stream Metabolism As An Efficient And Effective Means To Gage The Health And Understand The Interworking Of Urban Streams In A Watershed Of Rock Island, Il, 2018 Augustana College, Rock Island Illinois
Functions Of Ecosystems: Stream Metabolism As An Efficient And Effective Means To Gage The Health And Understand The Interworking Of Urban Streams In A Watershed Of Rock Island, Il, Ryan Johnson, Dr. Kevin Geedey
Celebration of Learning
Stream metabolism is a critical functional measure of stream health that integrates physical parameters like slope and discharge, with ecosystem functions like photosynthesis and respiration. Stream metabolism is widely studied; however, urban stream metabolism remains poorly understood. Stream metabolism was measured for five streams ranging from 1st to 5th orders from October 11th to October 18th 2017 and four streams ranging from 1st to 4th order from October 22nd to 25th 2017 located within an approximately 9.3 square kilometer watershed of Rock Island, IL that has an urban to suburban type ...
Synechococcus As An Hooh-Consuming Helper For Prochlorococcus, 2018 University of Tennessee, Knoxville
Synechococcus As An Hooh-Consuming Helper For Prochlorococcus, Abigail Mcgettigan Jarratt
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
The marine cyanobacterium Prochlorococcus is an abundant and globally important microbe that contributes an estimated 40% of bacterial production in the oligotrophic ocean. The success of this photosynthetic bacterium is largely a consequence of its small cell size and streamlined genome, which are advantageous in nutrient-limited environments. However, this genomic streamlining has also led to the loss of the gene (katG) encoding catalase, an enzyme that is essential for the degradation and detoxification of the reactive oxygen species hydrogen peroxide (HOOH). HOOH is naturally present in the illuminated waters of the ocean, and in the absence of catalase, Prochlorococcus is ...
Examining Manganese Oxidation In Bacteria Through Bioinformatics And Environmental Sampling, 2018 Lynchburg College
Examining Manganese Oxidation In Bacteria Through Bioinformatics And Environmental Sampling, T'Onna Collins
Undergraduate Theses and Capstone Projects
Manganese is an essential element for all living things. Manganese plays a role in geochemical cycles, like the nitrogen fixation cycle and the oxygen-evolving complex. The focus of this study is the manganese oxidation mechanism. Manganese oxidation plays a role in metabolic and antioxidant processes. Pseudomonas putida GB-1 is commonly used to study the process of manganese oxidation in bacteria. We used the previously identified Mn-oxidase genes, mopA, mnxG and mcoA, from P. putida GB-1 to look for conservation of manganese oxidation in other organisms. Basic Local Alignment Search Tool (BLAST) was used to find homologs of these three genes ...
Sulfur Cave (Romania), An Extreme Environment With Microbial Mats In A Co2-H2S/O2 Gas Chemocline Dominated By Mycobacteria, 2018 California State University, Chico
Sulfur Cave (Romania), An Extreme Environment With Microbial Mats In A Co2-H2S/O2 Gas Chemocline Dominated By Mycobacteria, Serban M. Sarbu, Joost W. Aerts, Jean-François Flot, Rob J.M. Van Spanning, Calin Baciu, Artur Ionescu, Boglárka M. Kis, Reka Incze, Sándor Sikó-Barabási, Zoltan Para, Botond Hegyeli, Nicu-Viorel Atudorei, Casey Barr, Kenneth Nealson, Ferenc L. Forray, Cristian Lascu, Emily J. Fleming, Wilbert Bitter, Radu Popa
International Journal of Speleology
Sulfur Cave (Puturosu Mountain, Romania) is an extreme environment, unique for displaying life in a gas chemocline. The lower part of the cave is filled with CO2, CH4, and H2S of mofettic origin, while the upper part contains air that floats above the heavier volcanic gasses. S° and H2SO4 (from sulfur-oxidation) cover the cave wall at and below the CO2-H2S:O2 gas/gas interface. On the cave wall, near the interface the pH is < 1 and unusual microbial biofilms occur on the rock’s surface. We provide context information on the geology, mineralogy, chemistry and biology to better understand this unique environment. We have used X-ray diffraction, optical microscopy, scanning electron microscopy with EDAX capabilities, stable isotope analysis and 16S and 18S rDNA amplicon sequencing. The most common taxa in the microbial biofilms are Mycobacteria, Acidithiobacillus and Ferroplasmaceae. Liquid water in this system originates solely from condensation of water ...
Optimizing Genetic Manipulation Of Methanogens Through Faster Cloning Techniques, 2018 University of Arkansas, Fayetteville
Optimizing Genetic Manipulation Of Methanogens Through Faster Cloning Techniques, Merrisa Jennings
Biological and Agricultural Engineering Undergraduate Honors Theses
Methanogenesis is the biological production of methane. Only anaerobic archaea known as methanogens are capable of such a metabolic feat. They have strict living conditions and substrate sources which determine their rate of metabolism. This is of particular importance from a greenhouse gas reduction perspective or biogas capturing perspective. One of the best ways to optimize methanogen methane production is via genetic manipulation. The current procedures are timely though, therefore a faster cloning processes should be developed. The objective of this study was to optimize a premade genetic transformation kit known as the Gibson Kit. The Gibson Kit was supposed ...
Chloride Salt Inhibition On Lipid Production In Wastewater-Grown Algae For Biofuel Production, 2018 University of Arkansas, Fayetteville
Chloride Salt Inhibition On Lipid Production In Wastewater-Grown Algae For Biofuel Production, Will Richardson
Biological and Agricultural Engineering Undergraduate Honors Theses
Algae are increasingly being recognized as useful organisms for many applications in today’s world. Their ability to remove nitrogen, phosphorus, and trace metals from water while adding oxygen to water makes them an attractive tertiary treatment technology in municipal wastewater treatment facilities. At the same time, algae produce lipids and carbohydrates that are useful for biofuel production, and they are not a human food crop unlike many biofuel feedstocks. In this study the effect of increased chloride concentrations in wastewater was assessed on the ability of two species of algae, Chlorella vulgaris and Scenedesmus dimorphus, to function as a ...
Microbiome Of Commercial Broilers Through Evisceration And Immersion Chilling, 2018 University of Arkansas, Fayetteville
Microbiome Of Commercial Broilers Through Evisceration And Immersion Chilling, John A. Handley
Theses and Dissertations
The United States poultry industry generated 38.6 billion pounds (17,500 metric ton) of meat in 2014 which averaged to 121 pounds (55 kg) per individual of the U.S that same year. Of that meat generated by the poultry industry, an estimated 1 million cases of Salmonellosis will occur. Out of the 1 million cases approximately 40, 000 to 50,000 will be confirmed cases by the CDC. Recently, the USDA has requested changes in the inspection process and are currently allowing processors more freedom to utilize innovation to drive the increase in safer and more desirable foods ...
Antibiotic Resistant Bacteria In Water Environments In Louisville, Kentucky., 2018 University of Louisville
Antibiotic Resistant Bacteria In Water Environments In Louisville, Kentucky., Amy Priest
College of Arts & Sciences Senior Honors Theses
Antibiotic resistant bacterial strains are an increasing problem, particularly in clinical health care settings. As a result, bacterial infections are becoming increasingly challenging to treat with more cases becoming life threatening. Aquatic environments facilitate microbial diversity and the transfer of genetic elements and thus may serve as a reservoir for antibiotic resistant microbes. Human misuse of antibiotics may further facilitate the spread of resistance in water environments. With little known about the bacteria communities in local water environments, this study aimed to learn more about these populations through the following aims: 1) identify the microbial community composition from water environments ...
Isolation And Identification Of A Chloromethane-Degrading Bacterium From A Strictly Anaerobic Mixed Culture, 2018 University of Tennessee, Knoxville
Isolation And Identification Of A Chloromethane-Degrading Bacterium From A Strictly Anaerobic Mixed Culture, Samiha Sadruddin
University of Tennessee Honors Thesis Projects
No abstract provided.
Composition And Function Of The Bacterial Consortium Associated With The Accessory Nidamental Gland Of The Hawaiian Bobtail Squid, Jessica Bertenshaw
Honors Scholar Theses
The bacterial consortium associated with the accessory nidamental gland (ANG) of the Hawaiian bobtail squid, Euprymna Scolopes is posited to confer symbiotic benefits to the eggs of the squid when bacteria are transferred from the gland of the mother to the jelly coat, which surrounds the embryo in the egg. To characterize the composition of this community bacterial isolates from the egg jelly coat and from the ANG were identified using 16S sequencing. To elucidate the function of these bacteria, egg development experiments were performed to determine the effect of antibiotics on egg survival and the composition of the bacterial ...
Characterizing Cultivable Bacteria From Trachymyrmex Septentrionalis Fungus Gardens, 2018 University of Connecticut
Characterizing Cultivable Bacteria From Trachymyrmex Septentrionalis Fungus Gardens, Hannah Beatty
Honors Scholar Theses
The relationship between the fungus-growing ant Trachymyrmex septentrionalis, its symbiotic cultivar fungus, and the transient and residential community of microorganisms is a diverse and complex symbiosis that has evolved over space and time. The fungus garden, comprised primarily of the cultivar fungus belonging to the family Leucocoprineae,provides an environment that hosts many bacteria, which may also play an important role in this symbiosis. Although it is known that Pseudonocardia bacteria defend the ant host against fungal pathogens, other species of bacteria that are present in these fungus gardens also likely contribute to this symbiosis. Previous studies of this system ...
Predator-By-Environment Interactions Mediate Bacterial Competition In The Dictyostelium Discoideum Microbiome, 2018 Washington University in St Louis
Predator-By-Environment Interactions Mediate Bacterial Competition In The Dictyostelium Discoideum Microbiome, R Fredrik Inglis, Odion Asikhia, Erica Ryu, David C. Queller, Joan E. Strassmann
Biology Faculty Publications & Presentations
Interactions between species and their environment play a key role in the evolution of diverse communities, and numerous studies have emphasized that interactions among microbes and among trophic levels play an important role in maintaining microbial diversity and ecosystem functioning. In this study, we investigate how two of these types of interactions, public goods cooperation through the production of iron scavenging siderophores and predation by the social amoeba Dictyostelium discoideum, mediate competition between two strains of Pseudomonas fluorescens that were co-isolated from D. discoideum. We find that although we are able to generally predict the competitive outcomes between strains based ...
The Effects Of Salt Concentration On Tetrahymena Thermophila Growth And Crp1 Gene Expression, Anna Sapone, Rachel Tremaine,, Katie Mchugh
Carroll College Student Undergraduate Research Festival
The use of salt to remove ice from roads has resulted in increased salinity in many freshwater lakes in North America. For this project, we wanted to explore how environmental salt concentrations influence the rate of growth of Tetrahymena thermophila and expression of the CRP1 gene. We hypothesized that if the sodium concentration is increased in the media, Tetrahymena growth would decrease and expression of the CRP1 gene would increase. The CRP1 gene encodes a protein that helps regulate calcium concentrations within a cell based on the concentration of sodium ions. The media of the experimental group was treated with ...
Interactions And Growth Dynamics Of Prochlorococcus, Rhodospirilaceae, And Alteromonas In A Coculture Environment, 2018 University of Tennessee, Knoxville
Interactions And Growth Dynamics Of Prochlorococcus, Rhodospirilaceae, And Alteromonas In A Coculture Environment, Vasily Giovanni Carniello
EURēCA: Exhibition of Undergraduate Research and Creative Achievement
Interactions between different species of microorganisms have a significant role in the growth dynamics within the environment. (Morris 08) The specifics mechanisms of these interactions, however, are overall poorly understood. The purpose of this study is to study the interactions between the heterotrophs Rhodospirilaceae (EZ54), and Alteromonas (EZ55) and the cyanobacterium Prochlorococcus (Vol 1). Here we establish the effects the cyanobacterium has on the growth dynamics of each of the heterotrophs as well as the effect the two heterotrophs have on one another.
Assay Of The Reverse Osmosis Purified Water In The Life Science Building At Bowling Green State University, Ohio, 2018 Bowling Green State University - Main Campus
Assay Of The Reverse Osmosis Purified Water In The Life Science Building At Bowling Green State University, Ohio, Nicholas Mendenhall, Scott O. Rogers, Neocles B. Leontis
Contaminated water sources can cause problems for scientific research and result in costly delays and failures of experiments. At Bowling Green State University, the reverse osmosis supply circulating in the Life Sciences Building has been measurably contaminated for nearly three years, corresponding to a change in servicing of the system. While servicing has been accelerated, the contamination in the system remains. The focus of this research was to identify the species of bacteria and fungi growing inside of the water system so that it might alert those servicing the system, and to begin to eliminate the contamination. Reverse osmosis water ...
Detection, Activity Measurement And Phylogeny Of Ureolytic Bacteria Isolated From Elasmobranch Tissue, 2018 The University of Southern Mississippi
Detection, Activity Measurement And Phylogeny Of Ureolytic Bacteria Isolated From Elasmobranch Tissue, Yimu Yang
Free-ranging marine elasmobranch tissue-associated micro-organisms were cultured from free-ranging Atlantic stingray (Dasyatis sabina) and Atlantic sharpnose sharks (Rhizoprionodon terraenovae). 16S rRNA gene phylogeny indicated bacteria community structure in both elasmobranchs were under phylum Proteobacteria, Firmicutes and Actinobacteria. By conducting split-plot ANOVA, we found the microbial richness is significantly different (P=0.0814) between two superorders of elasmobranch, which may largely due to their preferred habitats and feeding habits. Urease presentence and activity was detected in phylogenetically diverse bacterial strains. Species with high urea-hydrolyzing ability, such as Micrococcus luteus (shark blood isolate: 46.84 mU/mg protein; stingray blood isolate: 24 ...
Tracking A Tree-Killer: Improving Detection And Characterizing Species Distribution Of Phytophthora Cinnamomi In Appalachian Forests, Kenton L. Sena
Theses and Dissertations--Plant and Soil Sciences
Phytophthora cinnamomi is a soil-borne oomycete pathogen causing root rot in susceptible host species. P. cinnamomi is thought to have originated in Southeast Asia, but has since been introduced to many regions around the world, where it causes dramatic declines in many forest tree species. In the eastern US, the primary susceptible tree species of concern are American chestnut (Castanea dentata), white oak (Quercus alba), and shortleaf pine (Pinus echinata). American chestnut, functionally eliminated in the early 1900s by the rapidly acting chestnut blight (Cryphonectria parasitica), has been the subject of decades-long breeding efforts aimed at improving chestnut resistance to ...
Photosynthetic Activity Under Low Light, 2018 The University of Akron
Photosynthetic Activity Under Low Light, Danijela Lonco
Honors Research Projects
Are bacteria capable of undergoing oxygenic photosynthesis under low-light conditions inside of caves? Inside of an Australian cave, Chlorophyll f was recently discovered. This chlorophyll pigment was found to demonstrate the capability for photosynthesis, suggesting that Chlorophyll f extends the spectrum of usable light needed for oxygenic photosynthesis (Lars, 2015). Due to the findings in this study, a team went to Carlsbad Caverns to see if there were any bacteria capable of undergoing oxygenic photosynthesis under low light.
Bioassessment Of A Drinking Water Reservoir Using Plankton: High Throughput Sequencing Vs. Traditional Morphological Method, 2018 Nanyang Normal University
Bioassessment Of A Drinking Water Reservoir Using Plankton: High Throughput Sequencing Vs. Traditional Morphological Method, Wanli Gao, Zhaojin Chen, Yuying Li, Yangdong Pan, Jingya Zhu, Shijun Guo, Lanqun Hu, Jin Huang
Environmental Science and Management Faculty Publications and Presentations
Drinking water safety is increasingly perceived as one of the top global environmental issues. Plankton has been commonly used as a bioindicator for water quality in lakes and reservoirs. Recently, DNA sequencing technology has been applied to bioassessment. In this study, we compared the effectiveness of the 16S and 18S rRNA high throughput sequencing method (HTS) and the traditional optical microscopy method (TOM) in the bioassessment of drinking water quality. Five stations reflecting different habitats and hydrological conditions in Danjiangkou Reservoir, one of the largest drinking water reservoirs in Asia, were sampled May 2016. Non-metric multi-dimensional scaling (NMDS) analysis showed ...
Abundance Of Methanosaeta Concilii-Like Species In The Sediment Of Ledbetter Creek Embayment Of Kentucky Lake, 2018 Murray State University
Abundance Of Methanosaeta Concilii-Like Species In The Sediment Of Ledbetter Creek Embayment Of Kentucky Lake, Juanita Von Dwingelo
Murray State Theses and Dissertations
Reservoirs, including Kentucky Lake, are unique in that they do not follow a natural cycle of rising during the rainy seasons and dropping during the dry summers. The lake is manipulated, mainly for flood control and transportation, so that the water level is increased during the summer months and decreased during the winter months. This manipulation leaves the littoral sediment of the lake submerged in the summer and exposed in the winter. The seasonal water level, along with other factors, like farming practices in the watershed, contributes to the availability of nutrients for organisms inhabiting the sediment of the lake ...