Open Access. Powered by Scholars. Published by Universities.®

Medicine and Health Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

2019

Veterinary Medicine

Institution
Keyword
Publication
Publication Type

Articles 391 - 400 of 400

Full-Text Articles in Medicine and Health Sciences

Genetic Characteristics Of Avian Influenza A Viruses Isolated From Thai Free-Grazing Ducks And Their Pathogenicity Potential In Animal Models, Supassama Chaiyawong Jan 2019

Genetic Characteristics Of Avian Influenza A Viruses Isolated From Thai Free-Grazing Ducks And Their Pathogenicity Potential In Animal Models, Supassama Chaiyawong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Influenza A viruses (IAVs) can infect several animal species and humans. In Thailand, free-grazing ducks are reservoir hosts of influenza A virus. The free-grazing ducks are potentially interface with wild birds and domestic birds and subsequently cause interspecies transmission of IAVs. This thesis composes of three study phases. Phase 1 is surveillance of influenza A in free-grazing ducks. Phase 2 is genetic characterization and diversity analysis of influenza A viruses from free-grazing ducks. Phase 3 is challenge of influenza A viruses isolated from free-grazing ducks in animal models. For phase 1, multi-years surveillance of IAVs in free-grazing ducks was conducted …

Go to article

Detection And Study Of Genetic Diversity Of Selected Vector-Borne Protozoa And Rickettsia In Buffaloes In Thailand By Biomolecular Method, Hoang Lan Anh Nguyen Jan 2019

Detection And Study Of Genetic Diversity Of Selected Vector-Borne Protozoa And Rickettsia In Buffaloes In Thailand By Biomolecular Method, Hoang Lan Anh Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to determine the occurrence, geographical distribution and genetic diversity of tick and mosquito-borne protozoa and rickettsia in buffaloes by the PCR – sequencing approach. A total of 456 blood samples were collected in provinces of Thailand including Mukdahan, Uthai Thani, Lampang, Amnat Charoen, Nong Bua Lamphu, Phatthalung, Surin and Chachoengsao. DNA was extracted from buffalo blood and screened for the presence of pathogens, followed by sequencing. The results indicated that the overall prevalence of rickettsia, protozoa, and malaria were 41%, 25.4%, and 12.9% respectively. Double and triple infection of buffaloes with pathogens were observed in 19.1% of …

Go to article

Effect Of Fish Antifreeze Protein Type Iii Supplementation To Semen Extender On Cryopreserved Dog Spermatozoa, Rudal Jetta Bayusalaksa Jan 2019

Effect Of Fish Antifreeze Protein Type Iii Supplementation To Semen Extender On Cryopreserved Dog Spermatozoa, Rudal Jetta Bayusalaksa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Ice binding protein such as antifreeze protein (AFP) type III have been used as a cryoprotectant and proven to improve sperm survival in some species but dog. Addition of Equex STM paste to semen freezing medium provides beneficial effects on cryopreserved dog sperm. Hence, the objectives of this study were to (a) evaluate the effects of AFP (Experiment I) and (b) its combination with Equex STM paste on cryopreserved dog spermatozoa (Experiment II). The semen samples were pooled to allow for a sufficient number of sperm and to reduce an individual variation. Only sperm-rich fractions with total motility more than …

Go to article

Genetic Characterization Of Canine Parvovirus And Circovirus In Dogs In Vietnam, Nguyen Manh Tuong Jan 2019

Genetic Characterization Of Canine Parvovirus And Circovirus In Dogs In Vietnam, Nguyen Manh Tuong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine parvovirus (CPV-2) emerged and caused severe enteritis in dogs since the 1970s. The last variant, CPV-2c is considered as the high virulent strain and spread ubiquitous worldwide. This strain was early detected in Vietnam since 2002, but the whole genome is still fragmentary. In this study, we attempted to gain the whole genome of CPV-2c from the Vietnamese infected dogs as the first report of the full-length CPV-2c sequence originated from Asia. The Vietnamese CPV-2c exhibited the common mutations in both structural and nonstructural proteins and shared a typical evolutionary pattern with Asian strains. Besides, Vietnamese sequences presented some …

Go to article

Establishment Of Encapsulated Human Dental Pulp Stem Cell-Derived Insulin Producing Cells (Hdpsc-Ipcs) For Translational Stem Cell Based Diabetes Therapy, Suryo Kuncorojakti Jan 2019

Establishment Of Encapsulated Human Dental Pulp Stem Cell-Derived Insulin Producing Cells (Hdpsc-Ipcs) For Translational Stem Cell Based Diabetes Therapy, Suryo Kuncorojakti

Chulalongkorn University Theses and Dissertations (Chula ETD)

Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp stem cell (hDPSC)-derived IPCs (hDPSC-IPCs) by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed. The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG- and ALGPA-encapsulated hDPSC-IPCs maintained viability for at least 336 hours and sustained …

Go to article

Detection And Differentiation Of Haemosporidian Parasites In Chickens (Gallus Gallus Domesticus) Using Molecular Diagnostic Approach, Thi Xuan Mai Nguyen Jan 2019

Detection And Differentiation Of Haemosporidian Parasites In Chickens (Gallus Gallus Domesticus) Using Molecular Diagnostic Approach, Thi Xuan Mai Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Haemosporidioses in domestic chicken (Gallus gallus domesticus) are not only widely distribute but causing high mortality and non-specific symptoms. The multiplex PCR assay developed in this study can concurrently detect and differentiate four haemosporidian parasites Leucocytozoon caulleryi, Leucocytozoon sabrazesi, Plasmodium juxtanucleare, and Plasmodium gallinaceum. Comparing to microscopic examination performed in parallel on 157 samples, multiplex PCR assay exhibited significantly higher detection rate in diagnosis of haemosporidians (p = 0.000). The results showed that, L. sabrazesi, P. juxtanucleare, and P. gallinaceum detection rate of multiplex PCR were all higher than that of microscopic examination with p = 0.002, 0.000, and 0.004, …

Go to article

Pcr-Based Detection And Differentiation Of Piroplasms And Malaria Parasites From Goats In Thailand, Tu Cam Luu Huynh Jan 2019

Pcr-Based Detection And Differentiation Of Piroplasms And Malaria Parasites From Goats In Thailand, Tu Cam Luu Huynh

Chulalongkorn University Theses and Dissertations (Chula ETD)

A novel multiplex PCR (mPCR) was developed to identify and differentiate malaria parasite (Plasmodium caprae), and two piroplasms (Babesia bigemina and Theileria luwenshuni) in Thai goats. Three pairs of primer were designed to be species-specific, targeting cytochrome c oxidase subunit I (COI) gene in mitochondria. Plasmids containing each parasite’s DNA was serially diluted to evaluate the sensitivity of singleplex and mPCR assays. The specificity of the primer sets was confirmed by testing for amplification from DNA of each pathogen and other related hemoparasites. A total of 100 goat blood samples from five provinces of Thailand was used to validate the …

Go to article

Resistome Analysis Of Aeromonas Veronii Strains Isolated From Diseased Outbreak In Tilapia Farms In Thailand, Rungnapa Sakulworakan Jan 2019

Resistome Analysis Of Aeromonas Veronii Strains Isolated From Diseased Outbreak In Tilapia Farms In Thailand, Rungnapa Sakulworakan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Aeromonas veronii outbreaks in tilapia farming caused relatively high mortality. Moreover, it was resistant to many kinds of antimicrobial used in Thailand aquaculture. According to no CLSI standard, the determination of antimicrobial efficacy has been limited phenotypically; the genomics study is required. This research aims to analyze the resistome of A. veronii isolated from diseased Tilapia in Chainat, Nong Khai and Uttaradit province, Thailand. Twelve isolates of A. veronii were identified base on gyrB sequencing then determined the MIC value to eight antimicrobials (AMP, AML, GEN, ENR, OXO, OXY, SXT, and FFC). According to MIC patterns, five representatives were performed …

Go to article

Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwanachai Jan 2019

Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwanachai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Osteoarthritis (OA) is found secondary from medial patellar luxation (MPL) which is one of the most common diseases in small-breed dogs. Managements of the disease are surgical correction or a medical treatment. In the patients who are unable to do the surgical correction, non-steroidal anti-inflammatory drugs (NSAIDs) remains the drug of choice for OA therapy. Nowadays nutraceuticals have become more popular since there are no side effects once given for a long period of time. The purpose of this study was to evaluate the clinical chondroprotective effects of undenatured collagen type-II (UCII) on OA secondary to canine medial patellar luxation. …

Go to article

Studies Of Beta-Cardiotoxin From King Cobra Venom On Rat Cardiac Functions: Effect On Isolated Single Cardiomyocyte, Tuchakorn Lertwanakarn Jan 2019

Studies Of Beta-Cardiotoxin From King Cobra Venom On Rat Cardiac Functions: Effect On Isolated Single Cardiomyocyte, Tuchakorn Lertwanakarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Beta-cardiotoxin (ß-CTX), a novel protein isolated from the King cobra (Ophiophagus hannah) venom has previously been proposed as a beta-blocker candidate. However, cellular mechanisms of ß-CTX on cardiomyocyte is unknown. This study aimed to evaluate the impact of ß-CTX on isolated rat cardiomyocyte function and the cardiac myofibrillar activity, and to explore involvement of ß-adrenergic receptor (ß-AR) signaling pathway. ß-CTX was isolated and purified using two-step chromatographic method and confirmed by N-terminal sequencer. The function of ß-CTX on cardiomyocyte was compared to propranolol in 3 conditions, basal state, with isoproterenol (ISO), and with forskolin (FSK). ß-CTX exhibited more potency than …

Go to article

First Prev