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Comparison Of Immunohistochemistry Methods For Visualization Of Middle Eastern Respiratory Viral Antigen, Elena Carlos Apr 2019

Comparison Of Immunohistochemistry Methods For Visualization Of Middle Eastern Respiratory Viral Antigen, Elena Carlos

Kansas State University Undergraduate Research Conference

Middle Eastern respiratory syndrome (MERS), caused by MERS coronavirus (MERS-CoV) first appeared in Saudi Arabia in 2012. Although there have been less than 2,400 cases reported, the case fatality rate is 35%. Dromedary camels are the known host animal for the virus. However, they are difficult to manage experimental animals. In a recent study, alpacas proved to be a suitable experimental animal to further characterize MERS-CoV infection. Our work is part of a wider project focused on studying the efficacy of MERS-CoV antigen and RNA labeling in infected alpaca tissues, when these tissues are preserved in novel modular alcohol fixatives …


Effects Of Medium Chain Fatty Acid Application In Swine Feed On Porcine Epidemic Diarrhea Virus, A. B. Lerner, R. A. Cochrane, J. T. Gebhardt, S. S. Dritz, C. K. Jones, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, E. Porter, J. Anderson, P. C. Gauger, D. R. Magstadt, J. Zhang, B. Bass, T. P. Karnezos, B. De Rodas, J. C. Woodworth Jan 2019

Effects Of Medium Chain Fatty Acid Application In Swine Feed On Porcine Epidemic Diarrhea Virus, A. B. Lerner, R. A. Cochrane, J. T. Gebhardt, S. S. Dritz, C. K. Jones, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. Bai, E. Porter, J. Anderson, P. C. Gauger, D. R. Magstadt, J. Zhang, B. Bass, T. P. Karnezos, B. De Rodas, J. C. Woodworth

Kansas Agricultural Experiment Station Research Reports

Medium chain fatty acid (MCFA) application has been identified as a promising strategy to decrease viral pathogen transmission in swine feed. Four experiments were conducted to: 1) determine if MCFAs are effective when applied to feed both prior to and after porcine epidemic diarrhea virus (PEDV) inoculation measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), 2) evaluate the effects of varying amounts and combinations of MCFA measured by qRT-PCR, and 3) evaluate selected MCFA treatments in a bioassay. In Exp. 1, treatments were arranged in a 2 × 2 + 1 factorial with the main effects of chemical treatment …