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Full-Text Articles in Medicine and Health Sciences
Development Of An Indirect Elisa To Detect Antibodies Against Actinobacillus Suis Using Boiled Whole Cell Antigen, Siriluck Juntautsa
Development Of An Indirect Elisa To Detect Antibodies Against Actinobacillus Suis Using Boiled Whole Cell Antigen, Siriluck Juntautsa
Chulalongkorn University Theses and Dissertations (Chula ETD)
Actinobacillus suis is considered an opportunistic pathogen in swine that can cause disease in animals of all ages, especially in high health status herds. The objective of this study was to developed an indirect enzyme-linked immunosorbent assay (ELISA) using boiled whole cell antigen for the detection of antibody against A. suis. The selected herd has clinical problems associated with A. suis infection. In addition, this herd was not received any Actinobacillus pleuropneumoniae (APP) vaccine nor diagnosed with APP infection before the study. The isolation of A. suis was recovered from lung of the infected pig in the selected herd. Moreover, …
Akt Expression And Lactose Synthesis During Heat Exposure In Saanen Goat, Nungnuch Saipin
Akt Expression And Lactose Synthesis During Heat Exposure In Saanen Goat, Nungnuch Saipin
Chulalongkorn University Theses and Dissertations (Chula ETD)
In tropical countries, high ambient temperature (HTa) is a fundamental factor that cause a decline in the mammary gland function in dairy animals. Dairy animals fed under HTa decreased milk yield (MY) as well as milk compositions. The current study focuses on the effect of HTa to decrease milk synthesis from natural and in vitro HTa condition. The first experiment was performed to investigate the effect of HTa from the natural condition in early lactating Saanen goat, 2-4 weeks of postpartum (2 PP-4 PP). Five animals were investigated during the winter season as control ambient condition. Six animals as the …
Single Fixed-Time Artificial Insemination With Fresh Or Frozen Thawed Semen In Pig, Pachara Pearodwong
Single Fixed-Time Artificial Insemination With Fresh Or Frozen Thawed Semen In Pig, Pachara Pearodwong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Single fixed-time AI is the breeding protocol inseminating the sows once with either fresh or frozen-thawed (FT) semen at fixed time after ovulation induction without estrus detection. To develop the success single fixed-time AI protocol in Thailand, factors affecting the efficacy of single fixed-time AI were evaluated. Additionally, we also evaluated the effect of using lecithin in cryopreservation extender on FT semen qualities in order to find the possibility to perform single fixed-time AI using sperm preserved in lecithin based extender. It was found that administration of 10 ug buserelin at 72 h after weaning decreased weaning-to-ovulation interval compared to …
Determination Of Feline Ab Blood Types In Bangkok And Vicinities By The Tube Test, Teerawee Sangkaew
Determination Of Feline Ab Blood Types In Bangkok And Vicinities By The Tube Test, Teerawee Sangkaew
Chulalongkorn University Theses and Dissertations (Chula ETD)
The most important blood group system in cats is the AB system, which is identified by the presence and absence of antibodies and the antigen on the surface of red blood cells. Blood type A, B and AB are recognized in cats worldwide. The presence of strong alloantibodies against type A in all type B cats, and those with high titer against type B in some type A cats can cause significant transfusion reactions when cats are transfused with mismatched blood. Frequencies of blood types in a cat population is essential for estimating risks of transfusion reactions. The objectives of …
Evidence Of Vertical And Horizontal Transmission Of Francisella Noatunensis Subsp. Orientalis In Hybrid Red Tilapia (Orerochromis Sp.), Vuong Viet Nguyen
Evidence Of Vertical And Horizontal Transmission Of Francisella Noatunensis Subsp. Orientalis In Hybrid Red Tilapia (Orerochromis Sp.), Vuong Viet Nguyen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Francisellosis caused by Franciscella orientalis (formerly named: Franciscella noatunensis subspecies orientalis) has been reported as one of the most important emergent diseases of warm water fish. However, little is known about its transmission, fastidious nature, survival, and persistence in multiple environments and global presence. This study aims to investigate vertical transmission, horizontal transmission by vectors and effect of other organisms on the outbreak of francisellosis in hybrid red tilapia. To prove the transmission of F. orientalis from subclinically infected tilapia mouthbrooders to their offspring through the current practice of fry production in tilapia hatcheries, experimentally infected hybrid red tilapia broodstock …
Longitudinal Survey Of Swine Influenza Virus Infection In A Pig Farm In Thailand, 2017-2018, Chanakarn Nasamran
Longitudinal Survey Of Swine Influenza Virus Infection In A Pig Farm In Thailand, 2017-2018, Chanakarn Nasamran
Chulalongkorn University Theses and Dissertations (Chula ETD)
Swine influenza cause by influenza A virus, is the one of the most important zoonotic disease of pigs. Swine influenza virus (SIV) infection in pigs causes both economic loss in pig production and public health problem. SIVs subtypes H1N1, H1N2 and H3N2 cause respiratory disease in pigs worldwide. The objectives of this thesis were to determine the dynamic of swine influenza virus subtypes circulation in a pig farm in 2017-2018 and to monitor genetic diversity of swine influenza virus in a pig farm. During January 2017 to November 2018, the nasal swab samples (n=436) and blood samples (n=436) were collected …
Monitoring Of Influenza A Viruses In Thailand And Pathogenicity In Dogs And Guinea Pigs, Ratanaporn Tangwangvivat
Monitoring Of Influenza A Viruses In Thailand And Pathogenicity In Dogs And Guinea Pigs, Ratanaporn Tangwangvivat
Chulalongkorn University Theses and Dissertations (Chula ETD)
Influenza A virus causes respiratory disease in many species such as birds, horses, pigs, dogs and cats as well as humans. This thesis consists of 5 phases. Phases 1 was monitoring of canine influenza virus infection in Thailand. Phase 2 was determining the intravenous pathogenicity index of Thai canine influenza virus. Phase 3, 4 and 5 were investigating the pathogenicity of canine influenza viruses in dogs, chickens and guinea pigs, respectivly. Our results showed that 0.97% of canine serum samples (9/932) and 1.20% of feline serum sample (1/79) were tested positive for influenza A antibodies by NP-ELISA. Six serum samples …
The Left Ventricular Functions And Heart Rate Variability In Dogs With Pulmonic Valvular Stenosis, Ploypanut Trikhun
The Left Ventricular Functions And Heart Rate Variability In Dogs With Pulmonic Valvular Stenosis, Ploypanut Trikhun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Pulmonic valvular stenosis (PS) is one of the most common congenital heart diseases in dogs that can lead to the cardiac maladaptation. Also, PS can affect the cardiac autonomic nervous system (ANS). However, clinical information regarding to systolic function and cardiac ANS alteration in PS dogs had not been fully elucidated. The objectives of this study were to evaluate cardiac electrical property, left ventricular (LV) function, and to assess cardiac ANS from heart rate variability (HRV) analysis in PS dogs compared with healthy dogs. The dogs in this study were divided into 2 groups, PS dogs (n=13) and healthy control …
The Invention Of Plastic Plate For The Semiquantitative Detection Of Somatic Cells In Farm Bulk Tank Milk, Piyarat Jiarpinitnun
The Invention Of Plastic Plate For The Semiquantitative Detection Of Somatic Cells In Farm Bulk Tank Milk, Piyarat Jiarpinitnun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Bulk tank milk somatic cell count (BTM SCC) is worldwide acceptable as an indicator of subclinical mastitis and milk quality at the farm level. Several countries determine the BTM SCC level for quality control and milk pricing. In Thailand, BTM SCC standard is set at 500,000 cells/ml and premium milk quality is set at less than 100,000 cells/ml. Milk collecting centers (MCCs) use CMT (California Mastitis Test) for screening raw milk quality. However, CMT has an error and high variation. This study aims to invent a new semiquantitative device for determining BTM SCC and to validate the device in comparison …
Risk Assessment Of Vibrio Parahaemolyticus In White Shrimp (Litopenaeus Vannamei) From Retail Market, Praphatsorn Yokyingyong
Risk Assessment Of Vibrio Parahaemolyticus In White Shrimp (Litopenaeus Vannamei) From Retail Market, Praphatsorn Yokyingyong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Vibrio parahaemolyticus is the leading cause of seafood borne gastroenteritis in many countries including Thailand. It is a gram-negative, halophilic bacterium which was normally found in estuarine environment and seafood. The infection usually occurred from consuming raw, inadequate cooked, or cross-contaminated seafood. Risk assessment is a scientific tool to evaluate health hazard from V. parahaemolyticus infection. In this study, a risk of V. parahaemolyticus infection from raw shrimp consumption (Litopenaeus vannamei) was conducted. This study estimated the prevalence and level of V. parahaemolyticus in 2 retail types across 6 provinces nationwide by 3-tubes MPN. Risk assessment from raw shrimp consumption …
Comparison Of The Physical Capacity Using 6-Minute Walk Test And Questionnaire Before And After Resection Of Elongated Soft Palate By Bipolar Sealing Device In French Bulldogs, Tamolwan Anyamaneecharoen
Comparison Of The Physical Capacity Using 6-Minute Walk Test And Questionnaire Before And After Resection Of Elongated Soft Palate By Bipolar Sealing Device In French Bulldogs, Tamolwan Anyamaneecharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Brachycephalic airway obstruction syndrome (BAOS) is an anatomical abnormality of upper airway commonly found in brachycephalic breeds leading to clinical signs and physical capacity impairment. The purposes of this study were to compare clinical signs and physical capacity in French bulldogs between before and after surgical correction using questionnaire and 6-minute walk test (6-MWT). Twenty-seven French bulldogs were enrolled in this study and divided into normal group (n=7) and BAOS group (n=20). History taking, clinical signs and questionnaire information were recorded and 6-MWT was performed in both groups. BAOS dogs had significantly higher severity of clinical signs from questionnaire with …
Short Term Effects Of Sildenafil In Treatment Of Pulmonary Hypertension In Degenerative Mitral Valve Disease Dogs, Karun Saetang
Short Term Effects Of Sildenafil In Treatment Of Pulmonary Hypertension In Degenerative Mitral Valve Disease Dogs, Karun Saetang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Degenerative mitral valve disease (DMVD) is the most common acquired cardiac disease in small breed dogs. Pulmonary hypertension (PH) is a common complication in DMVD that can worsen the clinical signs, cardiac function, and median survival time of DMVD dogs. Conventional therapy of DMVD can reduce systolic pulmonary arterial pressure (sPAP) minimally. Thus, additional therapy is required. Sildenafil is used to treatment of PH in various causes, but the effect of sildenafil in the treatment of PH secondary to DMVD is not published yet. Therefore, this study aimed to evaluate the effects of sildenafil in combination with conventional therapy in …
Assessment Of Left Atrial Function In Feline Hypertrophic Cardiomyopathy By Using Two Dimensional Speckle Tracking Echocardiography, Arisara Kiatsilapanan
Assessment Of Left Atrial Function In Feline Hypertrophic Cardiomyopathy By Using Two Dimensional Speckle Tracking Echocardiography, Arisara Kiatsilapanan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Hypertrophic cardiomyopathy (HCM) is one of the most common myocardial diseases in cats. The left atrial (LA) function plays an essential role in compensatory mechanism of left ventricle with diastolic dysfunction to maintain cardiac output in cats affected with HCM. Nowadays, there are several echocardiographic techniques used to evaluate the left atrial function; however, each technique has limitations. To overcome the limitations, two-dimensional speckle tracking echocardiography (2D-STE) is a novel technique for assessment the LA function. To date, no study focusing on assessment of the left atrial function by 2D-STE in feline HCM has been reported. The objective of this …
Vector Competence Of Culex Tritaeniorhynchus For Duck Tembusu Virus, Jitra Sanisuriwong
Vector Competence Of Culex Tritaeniorhynchus For Duck Tembusu Virus, Jitra Sanisuriwong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Duck Tembusu virus (DTMUV), an emerging infectious disease in ducks, belongs to the Ntaya virus group of the Flavivirus genus in the Flaviviridae family. The emergence of DTMUV has been observed in layer and breeder duck farms in China since 2010 and in layer and broiler duck farms in Thailand since 2013. Infected ducks show neurologic signs, including an incapability to stand, ataxia, and paralysis. A significant drop in egg production is usually observed among layer ducks. The transmission of DTUMV involves mosquito vectors, however, the exact role of mosquitoes in the ecology of DTMUV in Thailand remains unclear. The …
Development Of Mucoadhesive Nanovaccine Against Columnaris Disease In Red Tilapia (Oreochromis Sp.), Sirikorn Kitiyodom
Development Of Mucoadhesive Nanovaccine Against Columnaris Disease In Red Tilapia (Oreochromis Sp.), Sirikorn Kitiyodom
Chulalongkorn University Theses and Dissertations (Chula ETD)
Tilapia (Oreochromis sp.) is very important and high production of freshwater fish in Thailand. Columnaris disease has been now recognized as one of the most serious infectious diseases in farmed tilapia. The disease is caused by Flavobacterium columnare. Among the prevention and control strategies, vaccination is one of the most effective approach. According to the pathogenesis of this bacteria, the characteristic lesion is almost at mucosal area of skin and gill. Therefore, hypothesized that the mucosal nanovaccine with mucoadhesive characteristic could be suitable vaccination method to control columnaris disease. In this study, we determined vaccine strain candidate by clinical field …
Epidemiological Surveillance Of Feline Morbillivirus Infection In The Aspects Of Polymerase Chain Reaction, Indirect Elisa And Immunohistochemistry, Surangkanang Chaiyasak
Epidemiological Surveillance Of Feline Morbillivirus Infection In The Aspects Of Polymerase Chain Reaction, Indirect Elisa And Immunohistochemistry, Surangkanang Chaiyasak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Feline morbillivirus (FeMV), a relatively new virus, has been discovered in domestic cats and associated with kidney diseases since 2012 in Hong Kong. The FeMV study gained widely attentions in many countries whereas the pathogenesis remains elusive. These studies aimed to provide the FeMV knowledge in Thai domestic cat by performing the genetic-based, serological-based and pathological-based studies of FeMV-infected in Thai cats. Additionally, the indirect enzyme linked immunosorbent assay (ELISA) and rabbit polyclonal antibody against FeMV matrix (FeMV-M) protein was produced for seroprevalence and viral localization by immunohistochemistry (IHC) techniques, respectively. Our results revealed the FeMV RNA prevalence at 11.9% …
Prevalence Of Enteric Helminths And Protozoa And Identification Of Hookworm, Threadworm And Giardia Spp. In Cats In Bangkok And Vicinity, Thailand, Wanarit Jitsamai
Prevalence Of Enteric Helminths And Protozoa And Identification Of Hookworm, Threadworm And Giardia Spp. In Cats In Bangkok And Vicinity, Thailand, Wanarit Jitsamai
Chulalongkorn University Theses and Dissertations (Chula ETD)
A total of 835 fecal samples collected from client-owned and community cats between 2014 and 2016 were subjected to wet fecal smear and/or PBS-ethyl acetate and/or ZnSO4 centrifugal flotation. Ancylostoma spp. was the most common, 28.5%, followed by 6.7% Toxocara spp., 5.3% Cystoisospora spp., 4.4% Platynosomum fastosum, 2.5% Taenia taeniaeformis, 1.7% Strongyloides spp., 1.4% Spirometra spp., 1.3% Dipylidium caninum, 0.1% Eucoleus aerophilus and 0.1% Opisthorchis-like trematode egg. For retroviruses, FeLV and FIV were positive 7.1% (19/269) and 5.2% (14/269), respectively, without association with endoparasitic infection. Based on Giardia copro-antigen detection test, 3.9% (9/233) of tested cats were positive. From multivariable …
Detection On The Expression Of The Transcription Factors: Oct-4, Nanog, And Sox-2 Genes In Canine Cutaneous Mast Cell Tumors, Sirilak Meesuwan
Detection On The Expression Of The Transcription Factors: Oct-4, Nanog, And Sox-2 Genes In Canine Cutaneous Mast Cell Tumors, Sirilak Meesuwan
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this study were to determine the expression of Oct-4, Nanog, and Sox-2 in both mRNA and proteins levels. The 30 biopsied MCT cases, from the Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University, were used in this study. The samples were divided into two parts; part I, the biopsied samples were processed for paraffin section and stained with H&E stain, for grading based on Patnaik’s system and Kiupel’s system and the other part was detected the expression of Oct-4, Nanog, and Sox-2 genes. The MCT samples were classified into three grades; grade I, II, and …
Correlation Of Allergen Specific Immunoglobulin To House Dust Mites And Commensal Microbes In Atopic Dogs And Identification Of Major Allergen In Dermatophagoides Farinae, Nathrada Khantavee
Correlation Of Allergen Specific Immunoglobulin To House Dust Mites And Commensal Microbes In Atopic Dogs And Identification Of Major Allergen In Dermatophagoides Farinae, Nathrada Khantavee
Chulalongkorn University Theses and Dissertations (Chula ETD)
To confirm a causative allergen in atopic dogs could test through intradermal skin testing (IDT) or allergen-specific IgE serology testing (ASIS). ASIS is more practical, convenient and safer. However, the results of ASIS could be affected by source and type of allergen. Crude allergen extracts were still used in commercial testing and protein antigens of skin microbes were not included in the panel of testing. In this study, our group developed serology test for confirming house dust mite allergy called Dermatophagoides farinae and D. pteronyssinus, and measuring specific immunoglobulin to skin microbes on dogs like Malssezia pachydermatis yeast and Staphylococcus …
Surveillance Of Economic And Public Health Important Respiratory And Enteric Viruses In Dogs And Pigs In Yangon, Myanmar, Pont Pont Mon
Surveillance Of Economic And Public Health Important Respiratory And Enteric Viruses In Dogs And Pigs In Yangon, Myanmar, Pont Pont Mon
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine infectious respiratory disease complex (CIRD) and Canine gastroenteritis (CGE) are major important diseases of dogs. Porcine Respiratory Disease complex (PRDC) and Porcine Enteric Disease complex (PEDC) are major economic and public health important diseases of pigs. The objectives of this study were 1) to detect and 2) to genetically characterize the economical and public health important respiratory and enteric viruses of dogs and pigs in Yangon, Myanmar during January, 2018 to October, 2019. In this thesis, 143 nasal swabs and 143 rectal swabs from 2 small animal clinics and 2 dog shelters were tested for canine respiratory viruses including …
Genetic Characteristics Of Avian Influenza A Viruses Isolated From Thai Free-Grazing Ducks And Their Pathogenicity Potential In Animal Models, Supassama Chaiyawong
Genetic Characteristics Of Avian Influenza A Viruses Isolated From Thai Free-Grazing Ducks And Their Pathogenicity Potential In Animal Models, Supassama Chaiyawong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Influenza A viruses (IAVs) can infect several animal species and humans. In Thailand, free-grazing ducks are reservoir hosts of influenza A virus. The free-grazing ducks are potentially interface with wild birds and domestic birds and subsequently cause interspecies transmission of IAVs. This thesis composes of three study phases. Phase 1 is surveillance of influenza A in free-grazing ducks. Phase 2 is genetic characterization and diversity analysis of influenza A viruses from free-grazing ducks. Phase 3 is challenge of influenza A viruses isolated from free-grazing ducks in animal models. For phase 1, multi-years surveillance of IAVs in free-grazing ducks was conducted …
Detection And Study Of Genetic Diversity Of Selected Vector-Borne Protozoa And Rickettsia In Buffaloes In Thailand By Biomolecular Method, Hoang Lan Anh Nguyen
Detection And Study Of Genetic Diversity Of Selected Vector-Borne Protozoa And Rickettsia In Buffaloes In Thailand By Biomolecular Method, Hoang Lan Anh Nguyen
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study aimed to determine the occurrence, geographical distribution and genetic diversity of tick and mosquito-borne protozoa and rickettsia in buffaloes by the PCR – sequencing approach. A total of 456 blood samples were collected in provinces of Thailand including Mukdahan, Uthai Thani, Lampang, Amnat Charoen, Nong Bua Lamphu, Phatthalung, Surin and Chachoengsao. DNA was extracted from buffalo blood and screened for the presence of pathogens, followed by sequencing. The results indicated that the overall prevalence of rickettsia, protozoa, and malaria were 41%, 25.4%, and 12.9% respectively. Double and triple infection of buffaloes with pathogens were observed in 19.1% of …
Effect Of Fish Antifreeze Protein Type Iii Supplementation To Semen Extender On Cryopreserved Dog Spermatozoa, Rudal Jetta Bayusalaksa
Effect Of Fish Antifreeze Protein Type Iii Supplementation To Semen Extender On Cryopreserved Dog Spermatozoa, Rudal Jetta Bayusalaksa
Chulalongkorn University Theses and Dissertations (Chula ETD)
Ice binding protein such as antifreeze protein (AFP) type III have been used as a cryoprotectant and proven to improve sperm survival in some species but dog. Addition of Equex STM paste to semen freezing medium provides beneficial effects on cryopreserved dog sperm. Hence, the objectives of this study were to (a) evaluate the effects of AFP (Experiment I) and (b) its combination with Equex STM paste on cryopreserved dog spermatozoa (Experiment II). The semen samples were pooled to allow for a sufficient number of sperm and to reduce an individual variation. Only sperm-rich fractions with total motility more than …
Genetic Characterization Of Canine Parvovirus And Circovirus In Dogs In Vietnam, Nguyen Manh Tuong
Genetic Characterization Of Canine Parvovirus And Circovirus In Dogs In Vietnam, Nguyen Manh Tuong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine parvovirus (CPV-2) emerged and caused severe enteritis in dogs since the 1970s. The last variant, CPV-2c is considered as the high virulent strain and spread ubiquitous worldwide. This strain was early detected in Vietnam since 2002, but the whole genome is still fragmentary. In this study, we attempted to gain the whole genome of CPV-2c from the Vietnamese infected dogs as the first report of the full-length CPV-2c sequence originated from Asia. The Vietnamese CPV-2c exhibited the common mutations in both structural and nonstructural proteins and shared a typical evolutionary pattern with Asian strains. Besides, Vietnamese sequences presented some …
Establishment Of Encapsulated Human Dental Pulp Stem Cell-Derived Insulin Producing Cells (Hdpsc-Ipcs) For Translational Stem Cell Based Diabetes Therapy, Suryo Kuncorojakti
Establishment Of Encapsulated Human Dental Pulp Stem Cell-Derived Insulin Producing Cells (Hdpsc-Ipcs) For Translational Stem Cell Based Diabetes Therapy, Suryo Kuncorojakti
Chulalongkorn University Theses and Dissertations (Chula ETD)
Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp stem cell (hDPSC)-derived IPCs (hDPSC-IPCs) by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed. The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG- and ALGPA-encapsulated hDPSC-IPCs maintained viability for at least 336 hours and sustained …
Detection And Differentiation Of Haemosporidian Parasites In Chickens (Gallus Gallus Domesticus) Using Molecular Diagnostic Approach, Thi Xuan Mai Nguyen
Detection And Differentiation Of Haemosporidian Parasites In Chickens (Gallus Gallus Domesticus) Using Molecular Diagnostic Approach, Thi Xuan Mai Nguyen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Haemosporidioses in domestic chicken (Gallus gallus domesticus) are not only widely distribute but causing high mortality and non-specific symptoms. The multiplex PCR assay developed in this study can concurrently detect and differentiate four haemosporidian parasites Leucocytozoon caulleryi, Leucocytozoon sabrazesi, Plasmodium juxtanucleare, and Plasmodium gallinaceum. Comparing to microscopic examination performed in parallel on 157 samples, multiplex PCR assay exhibited significantly higher detection rate in diagnosis of haemosporidians (p = 0.000). The results showed that, L. sabrazesi, P. juxtanucleare, and P. gallinaceum detection rate of multiplex PCR were all higher than that of microscopic examination with p = 0.002, 0.000, and 0.004, …
Pcr-Based Detection And Differentiation Of Piroplasms And Malaria Parasites From Goats In Thailand, Tu Cam Luu Huynh
Pcr-Based Detection And Differentiation Of Piroplasms And Malaria Parasites From Goats In Thailand, Tu Cam Luu Huynh
Chulalongkorn University Theses and Dissertations (Chula ETD)
A novel multiplex PCR (mPCR) was developed to identify and differentiate malaria parasite (Plasmodium caprae), and two piroplasms (Babesia bigemina and Theileria luwenshuni) in Thai goats. Three pairs of primer were designed to be species-specific, targeting cytochrome c oxidase subunit I (COI) gene in mitochondria. Plasmids containing each parasite’s DNA was serially diluted to evaluate the sensitivity of singleplex and mPCR assays. The specificity of the primer sets was confirmed by testing for amplification from DNA of each pathogen and other related hemoparasites. A total of 100 goat blood samples from five provinces of Thailand was used to validate the …
Resistome Analysis Of Aeromonas Veronii Strains Isolated From Diseased Outbreak In Tilapia Farms In Thailand, Rungnapa Sakulworakan
Resistome Analysis Of Aeromonas Veronii Strains Isolated From Diseased Outbreak In Tilapia Farms In Thailand, Rungnapa Sakulworakan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Aeromonas veronii outbreaks in tilapia farming caused relatively high mortality. Moreover, it was resistant to many kinds of antimicrobial used in Thailand aquaculture. According to no CLSI standard, the determination of antimicrobial efficacy has been limited phenotypically; the genomics study is required. This research aims to analyze the resistome of A. veronii isolated from diseased Tilapia in Chainat, Nong Khai and Uttaradit province, Thailand. Twelve isolates of A. veronii were identified base on gyrB sequencing then determined the MIC value to eight antimicrobials (AMP, AML, GEN, ENR, OXO, OXY, SXT, and FFC). According to MIC patterns, five representatives were performed …
Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwanachai
Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwanachai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Osteoarthritis (OA) is found secondary from medial patellar luxation (MPL) which is one of the most common diseases in small-breed dogs. Managements of the disease are surgical correction or a medical treatment. In the patients who are unable to do the surgical correction, non-steroidal anti-inflammatory drugs (NSAIDs) remains the drug of choice for OA therapy. Nowadays nutraceuticals have become more popular since there are no side effects once given for a long period of time. The purpose of this study was to evaluate the clinical chondroprotective effects of undenatured collagen type-II (UCII) on OA secondary to canine medial patellar luxation. …
Studies Of Beta-Cardiotoxin From King Cobra Venom On Rat Cardiac Functions: Effect On Isolated Single Cardiomyocyte, Tuchakorn Lertwanakarn
Studies Of Beta-Cardiotoxin From King Cobra Venom On Rat Cardiac Functions: Effect On Isolated Single Cardiomyocyte, Tuchakorn Lertwanakarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Beta-cardiotoxin (ß-CTX), a novel protein isolated from the King cobra (Ophiophagus hannah) venom has previously been proposed as a beta-blocker candidate. However, cellular mechanisms of ß-CTX on cardiomyocyte is unknown. This study aimed to evaluate the impact of ß-CTX on isolated rat cardiomyocyte function and the cardiac myofibrillar activity, and to explore involvement of ß-adrenergic receptor (ß-AR) signaling pathway. ß-CTX was isolated and purified using two-step chromatographic method and confirmed by N-terminal sequencer. The function of ß-CTX on cardiomyocyte was compared to propranolol in 3 conditions, basal state, with isoproterenol (ISO), and with forskolin (FSK). ß-CTX exhibited more potency than …