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Full-Text Articles in Medicine and Health Sciences
A Model For The Prediction Of Antimicrobial Resistance In Escherichia Coli Based On A Comparative Evaluation Of Fatty Acid Profiles, Randal S. Stahl, Bledar Bisha, Sebabrata Mahapatra, Jeffrey C. Chandler
A Model For The Prediction Of Antimicrobial Resistance In Escherichia Coli Based On A Comparative Evaluation Of Fatty Acid Profiles, Randal S. Stahl, Bledar Bisha, Sebabrata Mahapatra, Jeffrey C. Chandler
USDA Wildlife Services: Staff Publications
Antimicrobial resistance is a threat to agricultural production and public health. In this proof-of-concept study, we investigated predicting antimicrobial sensitive/resistant (S/R) phenotypes and host sources of Escherichia coli (n = 128) based on differential fatty acid abundance. Myristic (14:0), pentadecanoic acid (15:0), palmitic (16:0), elaidic (18:19) and steric acid (18:0) were significantly different (α = 0.05) using a two-way ANOVA for predicting nalidixic acid, ciprofloxacin, aztreonam, cefatoxime, and ceftazidime S/R phenotypes. Additionally, analyses of palmitoleic (16:1), palmitic acid (16:0), methyl palmitate (i-17:0), and cis-9,10-methyleneoctadecanoic acid (19:0Δ) showed these markers were significantly different (α = 0.05) between isolates obtained from cattle …
Identification Of Streptococcus Spp. Isolated From Bovine Milk And Characterization Of Their Antimicrobial Susceptibility Profiles In Taiwan, Jui-Chun Hsieh, Yu-Shan Yen, Shih-Te Chuang
Identification Of Streptococcus Spp. Isolated From Bovine Milk And Characterization Of Their Antimicrobial Susceptibility Profiles In Taiwan, Jui-Chun Hsieh, Yu-Shan Yen, Shih-Te Chuang
The Thai Journal of Veterinary Medicine
Bovine mastitis has had a great impact on the economic losses of the dairy industry. Nowadays, environmental pathogens cause the main types of bovine mastitis, especially the streptococci. The aim of the study was to identify the major Streptococcus species causing bovine mastitis in Taiwan as well as to reveal their antimicrobial susceptibility profiles. By colony morphology identifying, Gram staining, and subculturing on Edwards agar, the preliminary identified streptococci were subjected to biochemical identification, latex agglutination tests and dick diffusion tests for susceptibility tests of 16 antimicrobial agents. Among a total of 157 strains, Streptococcus uberis was the most dominant …
Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho
Recent Antimicrobial Susceptibility Of Lawsonia Intracellularis Field Isolates From Pigs With Proliferative Hemorrhagic Enteropathy In Korea, Byoung-Joo Seo, Sung-Hyun Moon, So-Min Lee, Seung-Yoon Lee, Byeong-Yeal Jung, Won-Il Kim, Chang-Seop Lee, Yeonsu Oh, Ho-Seong Cho
The Thai Journal of Veterinary Medicine
The study was performed to investigate the antimicrobial susceptibility of L. intracellularis field isolates from Korean pig farms. The novel four field isolates successfully obtained from clinical cases were prepared in IEC-18 cells to conduct the in vitro antimicrobial susceptibility testing (AST) by determining minimum inhibitory concentrations (MIC)s; either intracellular (InMIC) or extracellular MICs (ExMIC). The final MICs were assessed by counting the number of heavily infected cells (HICs;> 30 bacteria per cell) using an immunoperoxidase monolayer assay. Enrofloxacin (0.125 to 0.25 μg/mL: InMIC and 2 to 16 μg/mL; ExMIC) presented the most notable antimicrobial susceptibility, and marbofloxacin (0.25 to …