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Articles 31 - 60 of 2044
Full-Text Articles in Life Sciences
Comparison Of Bacterial And Archaeal Communities In The Subsurface Versus Surface: Implications For Nitrogen Cycling, Jason C. Kimble 5059801155
Comparison Of Bacterial And Archaeal Communities In The Subsurface Versus Surface: Implications For Nitrogen Cycling, Jason C. Kimble 5059801155
Biology ETDs
Arid-land caves are thought to be extremely nitrogen-limited, but almost nothing is known about how microbes in subsurface arid-land environments obtain this essential element to meet cellular demand. The depth of caves beneath the surface may represent a critical factor affecting microbial nitrogen cycling in these environments. Percolation of water and nutrients from a precipitation pulse event would affect deep arid-land carbonate caves at a much slower rate. To obtain nitrogen in deep, carbonate caves, microorganisms could use fixed N in the host rock for assimilatory biochemical pathways or for a respiratory electron acceptor. However, the latter process leads to …
The Role Of Orphan Nuclear Receptor Dax-1 (Nr0b1) In Human Breast Cancer Cells: Expression, Proliferation And Metastasis, Erin Dishington
The Role Of Orphan Nuclear Receptor Dax-1 (Nr0b1) In Human Breast Cancer Cells: Expression, Proliferation And Metastasis, Erin Dishington
Master's Theses
The orphan nuclear hormone receptor DAX-1 (Dosage Sensitive Sex Reversal, Adrenal Hypoplasia Congenita on the X Chromosome, gene 1) plays an important role in the development of adrenal and gonadal tissues and functions as a global negative-regulator of steroidogenesis. In addition, it is known to be involved in several diseases including some cancers. Herein, we describe our examination of the role of DAX-1 in breast cancer, specifically its influence on proliferation and metastasis and its expression during progressive stages of disease. In an effort to understand how DAX-1 influences breast cancer cell proliferation and metastasis, we used MCF7 breast cancer …
Vertebrate Body Size Jumps The Wright Way, Peter J. Wagner
Vertebrate Body Size Jumps The Wright Way, Peter J. Wagner
School of Biological Sciences: Faculty Publications
Fossil data provide copious evidence that anatomical (morphological) change within individual lineages is not constant: short bursts of great change commonly separate long durations of little change (1, 2). Despite this, most models of morphological change assume that probabilities of morphological character change are the same from one generation to the next: even if rates change over time—such as in “Cambrian explosion” or “early burst” models, where high disparity among anatomies evolves early—then it is still assumed that fast rates of continuous character change gave way to slow rates of continuous change (3). In statistical parlance, early bursts usually are …
Nitrogen Addition And Ecosystem Functioning: Both Species Abundances And Traits Alter Community Structure And Function, Anna R. Tatarko, Johannes M.H. Knops
Nitrogen Addition And Ecosystem Functioning: Both Species Abundances And Traits Alter Community Structure And Function, Anna R. Tatarko, Johannes M.H. Knops
School of Biological Sciences: Faculty Publications
Increased nutrient inputs can cause shifts in plant community composition and plant functional traits, both of which affect ecosystem function. We studied community- and species-level leaf functional trait changes in a full factorial nitrogen (N), phosphorus (P), and potassium (K) fertilization experiment in a semi-arid grassland. Nitrogen was the only nutrient addition to significantly affect leaf functional traits, and N addition increased community-weighted specific leaf area (SLA) by 19%, leaf chlorophyll content by 34%, height by 26%, and leaf dry matter content (LDMC) decreased by 11% while leaf thickness and toughness did not change significantly. At the species level, most …
Nanostructured Apatite-Mullite Glass-Ceramics For Enhanced Primary Human Osteoblast Cell Response, Gordon Cooke, Conor Dunne, Sarah Keane, Daithi De Faoite, Seamas Donnelly, Kenneth Stanton
Nanostructured Apatite-Mullite Glass-Ceramics For Enhanced Primary Human Osteoblast Cell Response, Gordon Cooke, Conor Dunne, Sarah Keane, Daithi De Faoite, Seamas Donnelly, Kenneth Stanton
Articles
This work investigates the difference in viability of primary human foetal osteoblast cells on a glass-ceramic surface with nanoscale topography relative to viability on a smooth glass-ceramic surface containing a bioactive phase. Apatite-mullite glass-ceramics containing bioactive fluorapatite (Ca10(PO4)6F2) and bioinert mullite (Si2Al6O13) were synthesised and subsequent heat-treatment was optimised to form nano-sized fluorapatite crystals. Etching was used to selectively remove the bioactive phase, producing a surface with disordered nanoscale topography. Cells were seeded onto a smooth polished glass-ceramic substrate with the bioactive phase intact, an etched …
Using Mixed Effects Modeling To Quantify Difference Between Patient Groups With Diabetic Foot Ulcers, Rachel French
Using Mixed Effects Modeling To Quantify Difference Between Patient Groups With Diabetic Foot Ulcers, Rachel French
Mahurin Honors College Capstone Experience/Thesis Projects
When diabetes progresses, many patients suffer from chronic foot ulcers. In a study described in Matrix Metalloproteinases and Diabetic Foot Ulcers (Muller et al., 2008), sixteen patients with diabetic foot ulcers were examined throughout a twelve week healing period. During this period, levels of matrix metalloproteinases (MMP-1), their inhibitors (TIMP-1), and the extracellular matrix in a wound area were measured at distinct time intervals for each patient. The ratios of these healing components are vital in determining whether a wound will heal or become chronic and never properly heal. Connecting Local and Global Sensitivities in a Mathematical Model for Wound …
Editorial: Murine Models Of Leukemia And Lymphoma, Christine E. Cutucache, Pierluigi Porcu
Editorial: Murine Models Of Leukemia And Lymphoma, Christine E. Cutucache, Pierluigi Porcu
Biology Faculty Publications
Murine models serve as an effective way to mimic the in vivo tumor microenvironmental interactions that take place in patients with leukemias and lymphomas. Specifically, leukemias and lymphomas rely heavily on the surrounding stroma and tissue microenvironmental cytokine and chemokine signals to ensure survival and expansion of tumor cells. Finally, leukemic cells migrate thanks to signals from varying regions of the host, furthering the progression and severity of disease. It is therefore impossible to fully understand such a dynamic relationship between tumor cells and their surrounding microenvironment, and the events to transformation in leukemias and lymphomas without an in vivo, …
Genetic Analysis Of The Major Capsid Protein Of The Archaeal Fusellovirus Ssv1: Mutational Flexibility And Conformational Change, Eric A. Iverson, David A. Goodman, Madeline E. Gorchels, Kenneth M. Stedman
Genetic Analysis Of The Major Capsid Protein Of The Archaeal Fusellovirus Ssv1: Mutational Flexibility And Conformational Change, Eric A. Iverson, David A. Goodman, Madeline E. Gorchels, Kenneth M. Stedman
Biology Faculty Publications and Presentations
Viruses with spindle or lemon-shaped virions are rare in the world of viruses, but are common in viruses of archaeal extremophiles, possibly due to the extreme conditions in which they thrive. However, the structural and genetic basis for the unique spindle shape is unknown. The best-studied spindle-shaped virus, Sulfolobus Spindle-shaped Virus 1 (SSV1), is composed mostly of the major capsid protein VP1. Similar to many other viruses, proteolytic cleavage of VP1 is thought to be critical for virion formation. Unlike half of the genes in SSV1, including the minor capsid protein gene VP3, the VP1 gene does not tolerate …
Top-Down And Bottom-Up Approaches To Understanding The Fate Of The Federally Endangered Schaus’ Swallowtail Butterfly (Heraclides Aristodemus Ponceanus), Jaeson T. Clayborn
Top-Down And Bottom-Up Approaches To Understanding The Fate Of The Federally Endangered Schaus’ Swallowtail Butterfly (Heraclides Aristodemus Ponceanus), Jaeson T. Clayborn
FIU Electronic Theses and Dissertations
The federally endangered Schaus’ swallowtail butterfly (Heraclides aristodemus ponceanus) populations have declined precipitously over the years. Despite tremendous efforts to augment the numbers of this butterfly through captive-bred releases and habitat enhancements, it remains imperiled and federally endangered. The dissertation’s objectives were: 1) to restore dry forest habitat in Biscayne National Park (BNP) for Schaus’ swallowtails; 2) to assess host plant (torchwood and wild lime) survivorship and growth in the restoration sites (BNP) and project dry forest habitat loss because of imminent sea level rise (SLR); 3) to quantify ant activity and record predator-prey interactions against Heraclides caterpillars …
Insights Into The Molecular Roles Of Zika Virus In Human Reproductive Complications And Congenital Neuropathologies, Rajendra Ghardbaran, Latchman Somenarain
Insights Into The Molecular Roles Of Zika Virus In Human Reproductive Complications And Congenital Neuropathologies, Rajendra Ghardbaran, Latchman Somenarain
Publications and Research
The recent upsurge in the association of congenital neurological disorders and infection by the Zika virus (ZIKV) has resulted in increased research focus on the biology of this flavivirus. Studies in animal models indicate that ZIKV can breach the placental barrier and selectively infect and deplete neuroprogenitor cells (NPCs) of the developing fetus, resulting in changes of brain structures, reminiscent of human microcephaly. In vitro and ex vivo studies using human cells and tissues showed that human NPCs and placental cells are targeted by ZIKV. Also of concern is the impact of ZIKV on human reproductive structures, with the potential …
Analysis Of Heart Muscle Tissue For Detection Of Fluorescently Labeled Cells In Conventional- And Frozen-Section Tissue Preparations, Lena Anna Mitchell
Analysis Of Heart Muscle Tissue For Detection Of Fluorescently Labeled Cells In Conventional- And Frozen-Section Tissue Preparations, Lena Anna Mitchell
Honors Theses
No abstract provided.
American Society Of Parasitologists Newsletter, Volume 39, Number 2, Fall/ Winter, 2017, Scott L. Gardner
American Society Of Parasitologists Newsletter, Volume 39, Number 2, Fall/ Winter, 2017, Scott L. Gardner
American Society of Parasitologists: Newsletter
Fall/Winter 2017 issue of the ASP newsletter.
Forage Selection And Habitat Architecture As Drivers Of Small Mammal Community Dynamics In An Arid, Nutrient Limited, Highly Stochastic Ecosystem, Jennifer Darby Noble
Forage Selection And Habitat Architecture As Drivers Of Small Mammal Community Dynamics In An Arid, Nutrient Limited, Highly Stochastic Ecosystem, Jennifer Darby Noble
Biology ETDs
In the Northern Chihuahuan desert, grassland and shrubland co-occur as separate stable states under similar climatic conditions. In this bottom up (resource driven) system, the magnitude and timing of precipitation events drives primary production which varies from year to year and season to season. Climate change is predicted to alter precipitation regimes, and increase aridity, facilitating shrub encroachment which results in increased landscape heterogeneity and a decrease in plant biodiversity. These changes will likely result in a restructuring of small mammal communities.
We used long-term data on precipitation, primary production, and abundances of small mammals in adjacent grassland and shrubland …
Neutral Genetic Variation In Adult Chinook Salmon (Oncorhynchus Tshawytscha) Affects Brain-To-Body Trade-Off And Brain Laterality, Mallory L. Wiper, Sarah J. Lehnert, Daniel D. Heath, Dennis M. Higgs
Neutral Genetic Variation In Adult Chinook Salmon (Oncorhynchus Tshawytscha) Affects Brain-To-Body Trade-Off And Brain Laterality, Mallory L. Wiper, Sarah J. Lehnert, Daniel D. Heath, Dennis M. Higgs
Great Lakes Institute for Environmental Research Publications
Low levels of heterozygosity can have detrimental effects on life history and growth characteristics of organisms but more subtle effects such as those on trade-offs of expensive tissues and morphological laterality, especially of the brain, have not been explicitly tested. The objective of the current study was to investigate how estimated differences in heterozygosity may potentially affect brain-to-body trade-offs and to explore how these heterozygosity differences may affect differential brain growth, focusing on directional asymmetry in adult Chinook salmon (Oncorhynchus tshawytscha) using the laterality and absolute laterality indices. Level of inbreeding was estimated as mean microsatellite heterozygosity resulting …
Developmental Nonlinearity Drives Phenotypic Robustness, Rebecca M. Green, Jennifer L. Fish, Nathan M. Young, Francis J. Smith, Benjamin Roberts, Katie Dolan, Irene Choi, Courtney L. Leach, Paul Gordon, James M. Cheverud, Charles C. Roseman, Trevor J. Williams, Ralph S. Marcucio, Benedikt Hallgrímsson
Developmental Nonlinearity Drives Phenotypic Robustness, Rebecca M. Green, Jennifer L. Fish, Nathan M. Young, Francis J. Smith, Benjamin Roberts, Katie Dolan, Irene Choi, Courtney L. Leach, Paul Gordon, James M. Cheverud, Charles C. Roseman, Trevor J. Williams, Ralph S. Marcucio, Benedikt Hallgrímsson
Biology: Faculty Publications and Other Works
Robustness to perturbation is a fundamental feature of complex organisms. Mutations are the raw material for evolution, yet robustness to their effects is required for species survival. The mechanisms that produce robustness are poorly understood. Nonlinearities are a ubiquitous feature of development that may link variation in development to phenotypic robustness. Here, we manipulate the gene dosage of a signaling molecule, Fgf8, a critical regulator of vertebrate development. We demonstrate that variation in Fgf8 expression has a nonlinear relationship to phenotypic variation, predicting levels of robustness among genotypes. Differences in robustness are not due to gene expression variance or …
Why Georeferencing Matters: Introducing A Practical Protocol To Prepare Species Occurrence Records For Spatial Analysis, Trevor D.S. Bloom, Aquila Flower, Eric G. Dechaine
Why Georeferencing Matters: Introducing A Practical Protocol To Prepare Species Occurrence Records For Spatial Analysis, Trevor D.S. Bloom, Aquila Flower, Eric G. Dechaine
Environmental Studies Faculty and Staff Publications
Species Distribution Models (SDMs) are widely used to understand environmental controls on species’ ranges and to forecast species range shifts in response to climatic changes. The quality of input data is crucial determinant of the model’s accuracy. While museum records can be useful sources of presence data for many species, they do not always include accurate geographic coordinates. Therefore, actual locations must be verified through the process of georeferencing. We present a practical, standardized manual georeferencing method (the Spatial Analysis Georeferencing Accuracy (SAGA) protocol) to classify the spatial resolution of museum records specifically for building improved SDMs. We used the …
A Redescription Of The Ichnospecies Koreanaornis Anhuiensis (Aves) From The Lower Cretaceous Qiuzhuang Formation At Mingguang City, Anhui Province, China, Li-Da Xing, Yuan-Chao Hu, Jian-Dong Huang, Qing He, Martin G. Lockley, Michael E. Burns, Jun Fang
A Redescription Of The Ichnospecies Koreanaornis Anhuiensis (Aves) From The Lower Cretaceous Qiuzhuang Formation At Mingguang City, Anhui Province, China, Li-Da Xing, Yuan-Chao Hu, Jian-Dong Huang, Qing He, Martin G. Lockley, Michael E. Burns, Jun Fang
Research, Publications & Creative Work
The Cretaceous bird trackway originally labeled Aquatilavipes anhuiensis, in 1994, had previously been examined, photographed and replicated, but never described or illustrated in detail. However, it has been part of a widening discussion about the distribution of Aquatilavipes and Koreanaornis in China (and Korea). Here we illustrate and formally describe the holotype in detail and assign it to Koreanaornis (Koreanaornis anhuiensis) as informally proposed by previous authors. We also demonstrate that most authenticated reports of Koreanaornis, including the Anhui occurrence, are from the Lower Cretaceous, not from the Upper Cretaceous as previously reported.
A Teachable Unit On Soft Corals With Instructional Method Variances, Emily Breech
A Teachable Unit On Soft Corals With Instructional Method Variances, Emily Breech
Honors Projects
The soft coral Sinularia flexibilis contains an aqueous alcohol extract that has shown antineoplastic activity against lymphocytic leukemia (Weinheimer and Matson, 1977). It also has other biological properties that work as antimicrobials, anti-inflammatory agents, and cytotoxicity activities (Kamel and Slattery, 2005). There is much research on the effects of flow rate on growth and morphology (Khalesi, Beeftink, & Wijffels, 2007) and the effects of light-dependency on growth rate (Khalesi, Beeftink, & Wijffels, 2009). The purpose of this student project was to further research on S. flexibilis, as well as incorporate that research into evaluating the effects of lecture-based teaching …
Mechanical Test Methods For Assessing Porcine Carotid And Uterine Artery Burst Pressure Following Ex Vivo Ultrasonic Ligature Seal And Transection, Carissa M. Krane, Margaret Pinnell, Courtney Gardner, Mercedes Thompson, James Coleman, Robert Wilkens
Mechanical Test Methods For Assessing Porcine Carotid And Uterine Artery Burst Pressure Following Ex Vivo Ultrasonic Ligature Seal And Transection, Carissa M. Krane, Margaret Pinnell, Courtney Gardner, Mercedes Thompson, James Coleman, Robert Wilkens
Robert J. Wilkens
A test method was developed to identify those variables important for assessing the performance of ultrasonic surgical devices in ex vivo ligature sealing of porcine carotid and uterine arteries. Ruggedness testing using a small sample size in pilot experiments was conducted using a newly developed test method in an effort to assess the usefulness of this methodology and to identify test variables that might warrant further testing. The development of this test method included the use of a custom-designed prototypic tension device for load-controlled ex vivo vessel stretching during saline perfusion and subsequent seal and transection of porcine arteries with …
Inhibitory Or Excitatory? Optogenetic Interrogation Of The Functional Roles Of Gabaergic Interneurons In Epileptogenesis, Hui Ye
Biology: Faculty Publications and Other Works
Alteration in the excitatory/inhibitory neuronal balance is believed to be the underlying mechanism of epileptogenesis. Based on this theory, GABAergic interneurons are regarded as the primary inhibitory neurons, whose failure of action permits hyperactivity in the epileptic circuitry. As a consequence, optogenetic excitation of GABAergic interneurons is widely used for seizure suppression. However, recent evidence argues for the context-dependent, possibly “excitatory” roles that GABAergic cells play in epileptic circuitry. We reviewed current optogenetic approaches that target the “inhibitory” roles of GABAergic interneurons for seizure control. We also reviewed interesting evidence that supports the “excitatory” roles of GABAergic interneurons in epileptogenesis. …
Connectivity Motifs Of Inhibitory Neurons In The Mouse Auditory Cortex, Hysell V. Oviedo
Connectivity Motifs Of Inhibitory Neurons In The Mouse Auditory Cortex, Hysell V. Oviedo
Publications and Research
Connectivity determines the function of neural circuits and it is the gateway to behavioral output. The emergent properties of the Auditory Cortex (ACx) have been difficult to unravel partly due to our assumption that it is organized similarly to other sensory areas. But detailed investigations of its functional connectivity have begun to reveal significant differences from other cortical areas that perform different functions. Using Laser Scanning Photostimulation we previously discovered unique circuit features in the ACx. Specifically, we found that the functional asymmetry of the ACx (tonotopy and isofrequency axes) is reflected in the local circuitry of excitatory inputs to …
The Rate Of Fungal Pathogen Growth (Botryosphaeria Dothidea) In Stems Of The Chaparral Shrub Rhus Integrifolia, Jayla D. Simmons, Edward J. Ayala, Kamara J. Satcher, Stephen D. Davis, Karagan L. Smith, Talia A. Cao
The Rate Of Fungal Pathogen Growth (Botryosphaeria Dothidea) In Stems Of The Chaparral Shrub Rhus Integrifolia, Jayla D. Simmons, Edward J. Ayala, Kamara J. Satcher, Stephen D. Davis, Karagan L. Smith, Talia A. Cao
Featured Research
The fungus Botryosphaeria dothidea will grow between one to five millimeters after a week on the chaparral species Rhus integrifolia. To test this, we inoculated six individual shrub located behind the greenhouse on Campus Walk Road . On each shrub we selected two branches, receiving adequate and equal sunlight and inoculated one branch with the fungus B. dothidea and the other with a plain gel in order to compare the growth with a control group. The data we collected at the end of the one week period was similar to our initial hypothesis. The shortest growth was on the …
Post-Drought Recovery In The Chaparral Shrub Ceanothus Spinosus, Katie C. Lindley, Kylie N. Smith, Nina R. Duchild, Stephen D. Davis, Karagan L. Smith, Talia A. Cao
Post-Drought Recovery In The Chaparral Shrub Ceanothus Spinosus, Katie C. Lindley, Kylie N. Smith, Nina R. Duchild, Stephen D. Davis, Karagan L. Smith, Talia A. Cao
Featured Research
This study investigated the abilities of Ceanothus spinosus, or greenbark ceanothus, to recover after a historic drought. This plant is one of the hardiest in the Santa Monica Mountains, providing soil stabilization and nutrients to other organisms in the ecosystem. In a previous investigation done during the drought, it was found that C. spinosus subjects growing in their natural habitat experienced significant dieback, with predawn water potentials falling as low as -7 MPa and native embolisms at an average of 47.17% (Ross et al., 2016). Other parameters tested were stomatal conductance, photosynthetic rate, and chlorophyll fluorescence. Compared to the …
Comparison Of Photosynthesis Among Three Chaparral Species In The Family Anacardiaceae, Catelyn T. Price, Sarah A. Holmes, Brandon T. Harris, Stephen D. Davis, Karagan L. Smith, Talia A. Cao
Comparison Of Photosynthesis Among Three Chaparral Species In The Family Anacardiaceae, Catelyn T. Price, Sarah A. Holmes, Brandon T. Harris, Stephen D. Davis, Karagan L. Smith, Talia A. Cao
Featured Research
Recent argumentative data showed a lack of niche differentiation amongst three Banksia species in Australia conducted by Lamont and Bergl, and the Anacardiaceae family in the native chaparral of the Santa Monica Mountains are supposed to be significantly similar1. Because of this we wanted to test if there would be a significant difference between the stomatal conductance, photosynthesis, and water potential in samples of the Rhus ovata, Rhus integrifolia, and Malosma laurina (Rhus laurina) species. We tested this idea by collecting the stomatal conductance and photosynthetic rates of each plant species in the same general location with …
Fates Of Rare Species Under Siege From Invasion: Persistence Of Coccinella Novemnotata Herbst In Western North America Alongside An Invasive Congener, Edward W. Evans
Fates Of Rare Species Under Siege From Invasion: Persistence Of Coccinella Novemnotata Herbst In Western North America Alongside An Invasive Congener, Edward W. Evans
Biology Faculty Publications
Species invading new geographic regions may threaten continued existence of similar, indigenous relatives, particularly those species whose rarity may reflect an already tenuous existence. The spectacular colonization of North America in recent decades by Coccinella septempunctata L. has generated widespread concern over potentially adverse effects on population viability of native species of ladybird beetles (Coccinellidae). Coccinella novemnotata Herbst in particular has been hypothesized to be at great risk, as this species apparently dwindled in numbers across much of North America during the twentieth century. Here results of sampling diverse habitats over three decades are examined to address the fate of …
Constrained Sequence Alignment, Kyle Daling
Constrained Sequence Alignment, Kyle Daling
WWU Honors College Senior Projects
Constrained Sequence Alignment: A new algorithm designed to help biologists produce better alignment for protein sequences.
Testing For The Presence Of Botryosphaeria Dothidea In Ceanothus Spinosus, Brandon R. Grinovich, Chelsea N. Puncochar, Katarina L. Carter, Stephen D. Davis, Rodney L. Honeycutt, Talia A. Cao, Karagan L. Smith
Testing For The Presence Of Botryosphaeria Dothidea In Ceanothus Spinosus, Brandon R. Grinovich, Chelsea N. Puncochar, Katarina L. Carter, Stephen D. Davis, Rodney L. Honeycutt, Talia A. Cao, Karagan L. Smith
Featured Research
Botryosphaeria dothidea is an opportunistic fungus found in some of the cavitated branches of the vegetation in the Santa Monica Mountains. In this study, we focused on finding the presence of Botryosphaeria dothidea in Ceanothus spinosus located on Pepperdine University’s campus. We wanted to test this hypothesis because we observed a large amount of dieback in the Ceanothus spinosus population and wanted to identify a possible contributor. Because of the large number of cavitated branches the Ceanothus spinosus contained, we hypothesized they contained fungus. To test this, we took twenty samples of twenty different Ceanothus spinosus, isolated the fungus …
Zinc Chloride Activates Phospho-Akt And Promotes Chondrocyte Maturation In The Atdc5 Chondrogenic Cell Line, Donya T. Burgess
Zinc Chloride Activates Phospho-Akt And Promotes Chondrocyte Maturation In The Atdc5 Chondrogenic Cell Line, Donya T. Burgess
Seton Hall University Dissertations and Theses (ETDs)
Fracture healing is characterized by inflammation, cartilage formation, bone deposition, and remodeling. Endochondral ossification is an important part of callus formation that relies on the maturation of chondrocytes. Early in their maturation these cells express collagen type II alpha 1 chain (COL2A1) and express collagen type X alpha 1 chain (COL10A1) as they mature further and become hypertrophic chondrocytes. Activation of the insulin pathway is considered a primary factor involved in the initiation of chondrogenesis and its progression during bone healing. Insulin mimetics like zinc chloride (ZnCl2) have been shown to improve bone healing outcomes and enhance bone …
Small Rna-Dependent Gene Silencing In The Green Alga Chlamydomonas Reinhardtii: Functions And Mechanisms, Eun Jeong Kim
Small Rna-Dependent Gene Silencing In The Green Alga Chlamydomonas Reinhardtii: Functions And Mechanisms, Eun Jeong Kim
School of Biological Sciences: Dissertations, Theses, and Student Research
Small RNAs (sRNAs), ~20-30 nucleotides in length, are non-coding RNAs that play essential roles in the regulation of gene expression in eukaryotes. They lead to inactivation of cognate sequences at the post-transcriptional level via a variety of mechanisms involved in translation inhibition and/or RNA degradation.
In the Chlorophyta Chlamydomonas reinhardtii, however, the molecular machinery responsible for sRNA-mediated translational repression remains unclear. To address the mechanisms of translation inhibition by sRNA, we have isolated an RNAi defective mutant (Mut26), which contains a deletion of the gene encoding the homolog of CCR4 in Chlamydomonas. We investigated the expression of …
The Toxicity Of Nanoparticles Depends On Multiple Molecular And Physicochemical Mechanisms, Yue-Wern Huang, Melissa Cambre, Han-Jung Lee
The Toxicity Of Nanoparticles Depends On Multiple Molecular And Physicochemical Mechanisms, Yue-Wern Huang, Melissa Cambre, Han-Jung Lee
Biological Sciences Faculty Research & Creative Works
Nanotechnology is an emerging discipline that studies matters at the nanoscale level. Eventually, the goal is to manipulate matters at the atomic level to serve mankind. One growing area in nanotechnology is biomedical applications, which involve disease management and the discovery of basic biological principles. In this review, we discuss characteristics of nanomaterials, with an emphasis on transition metal oxide nanoparticles that influence cytotoxicity. Identification of those properties may lead to the design of more efficient and safer nanosized products for various industrial purposes and provide guidance for assessment of human and environmental health risk. We then investigate biochemical and …