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Articles 1 - 30 of 95
Full-Text Articles in Life Sciences
Exogenously Sourced γ-Irradiated Chitosan-Mediated Regulation Of Growth, Physiology, Quality Attributes And Yield In Mentha Piperita L., Bilal Ahmad, Mohammad Masroor Akhtar Khan, Hassan Jaleel, Yawar Sadiq, Asfia Shabbir, Moin Uddin
Exogenously Sourced γ-Irradiated Chitosan-Mediated Regulation Of Growth, Physiology, Quality Attributes And Yield In Mentha Piperita L., Bilal Ahmad, Mohammad Masroor Akhtar Khan, Hassan Jaleel, Yawar Sadiq, Asfia Shabbir, Moin Uddin
Turkish Journal of Biology
In view of the increasing demand for essential oils from medicinal and aromatic plants, the application of irradiated polysaccharides like chitosan is a promising option. A pot experiment was performed to study the influence of various concentrations of irradiated chitosan (ICN) on growth, physiological, and yield parameters of Mentha piperita L., a plant with considerable pharmaceutical importance. The treatments were foliar spray of deionized water only (control), foliar spray of unirradiated chitosan (40 mg L-1), and ICN at 40, 80, 120, and 160 mg L-1. Among these, 80 mg L-1 ICN (treatment 4) proved to be the best for most …
Exploration Of The Binding Pocket Of Histone Deacetylases: The Design Of Potent And Isoform-Selective Inhibitors, Abdullahi̇ İbrahi̇m Uba, Kemal Yelekçi̇
Exploration Of The Binding Pocket Of Histone Deacetylases: The Design Of Potent And Isoform-Selective Inhibitors, Abdullahi̇ İbrahi̇m Uba, Kemal Yelekçi̇
Turkish Journal of Biology
Histone deacetylases (HDACs) are enzymes that act on histone proteins to remove the acetyl group and thereby regulate the chromatin state. HDACs act not only on histone protein but also nonhistone proteins that are key players in cellular processes such as the cell cycle, signal transduction, apoptosis, and more. "Classical" HDACs have been shown to be promising targets for anticancer drug design and development. However, the selectivity of HDAC inhibitors for HDAC isoforms remains the motivation of current research in this field. Here, we explored Class I HDACs and HDAC6 by sequence alignment and structural superimposition, catalytic channel extraction, and …
Induction Of Haploid Plants In Citrus Through Gamma-Irradiated Pollen And Ascertainment Of Ovule Age For Maximum Recovery Of Haploid Plantlets, Manoj Kundu, Anil Dubey, Manish Srivastav, Surendra Kumar Malik
Induction Of Haploid Plants In Citrus Through Gamma-Irradiated Pollen And Ascertainment Of Ovule Age For Maximum Recovery Of Haploid Plantlets, Manoj Kundu, Anil Dubey, Manish Srivastav, Surendra Kumar Malik
Turkish Journal of Biology
The present investigation was carried out for the induction of haploid plants in Citrus grandis through in situ parthenogenesis by pollination with gamma-irradiated pollen of C. limetta and C. sinensis, treated with 50, 100, 200, 300, and 400 Gy gamma ray doses followed by in vitro ovule culture. Ovule culture at 50 days after pollination (DAP) was found optimum for the maximum recovery of in vitro-raised plantlets as compared to 35 and 20 DAP. At 50 DAP, irrespective of the pollen parent, plantlet regeneration capacity decreased at 300 and 400 Gy irradiation treatment (0.62% and 0.60%, respectively) with maximum recovery …
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Turkish Journal of Biology
The presence of uninhibited side effects of cancer drugs often used in cancer treatment has stimulated the search for alternative therapeutic approaches. Therefore, anticarcinogenic effects of synthetic, herbal, and fungal drugs have been investigated for the treatment of various cancer types in recent studies. Lichens, symbiotic organisms of fungi and algae, synthesize metabolites with significant biological activities. The aim of the current study was to screen the anticancer potential of usnic acid on various types of nonmalignant cell lines (Vero, L929) and cancer cell lines (CaCo2, RD, Hep2C, HepG2, Wehi). The growth inhibitory effect of usnic acid was determined by …
Histomorphometric Evaluation Of The Testicular Parenchyma Of Rats Submittedto Protein Restriction During Intrauterine And Postnatal Life, Jessica Oliveira, Alluanan Silva, Sandra Souza, Rosana Morais, Elizabeth Neves Melo, Frederico Maia, Valdemiro Silva Junior
Histomorphometric Evaluation Of The Testicular Parenchyma Of Rats Submittedto Protein Restriction During Intrauterine And Postnatal Life, Jessica Oliveira, Alluanan Silva, Sandra Souza, Rosana Morais, Elizabeth Neves Melo, Frederico Maia, Valdemiro Silva Junior
Turkish Journal of Biology
The critical period of development is highly susceptible to disorders. Environmental contaminants, stress, and poor nutrition may permanently affect structurally and functionally an organism during adulthood. Protein restriction in intrauterine and neonatal periods may impair testicular cells and reduce steroidogenic activity. The current study investigated the effect of low protein diet during intrauterine and postnatal life on testicular function in immature and adult rats. Pregnant Wistar rats were fed either a normal protein diet or a low protein diet during pregnancy until birth and during lactation until weaning. The results showed that undernutrition during intrauterine life reduced epithelium height, tubular …
Cell-Compatible Phb/Silk Fibroin Composite Nanofibermat For Tissue Engineering Applications, Zeynep Karahali̇loğlu
Cell-Compatible Phb/Silk Fibroin Composite Nanofibermat For Tissue Engineering Applications, Zeynep Karahali̇loğlu
Turkish Journal of Biology
Cell compatibility is one of the prominent requirements for the fabrication of tissue engineering materials. Silk fibroin (SF) is an excellent material for biomedical applications and shows desirable properties such as good compatibility, minimal tissue reaction, and tailorable degradability. Poly[(R)-3-hydroxybutyrate] (PHB) has a high percentage of crystallinity and a high melting temperature. In this study, PHB and SF were blended together to improve the mechanical properties of the SF fibrous structure and the crystallinity of PHB. Furthermore, the cell-biomaterial interaction on the PHB/SF composite scaffold was expected to be enhanced via the SF. For this purpose, PHB/SF composite scaffolds were …
Identification And In Silico Analysis Of Galns Mutations Causing Morquio A Syndrome In Eight Consanguineous Families, Irfan Ullah, Abdul Nasir, Sarmad Mehmood, Sohail Ahmed, Muhammad Ikram Ullah, Asmat Ullah, Abdul Aziz, Syed Irfan Raza, Khadim Shah, Saad Ullah Khan, Muhammad Jawad Hassan, Wasim Ahmad
Identification And In Silico Analysis Of Galns Mutations Causing Morquio A Syndrome In Eight Consanguineous Families, Irfan Ullah, Abdul Nasir, Sarmad Mehmood, Sohail Ahmed, Muhammad Ikram Ullah, Asmat Ullah, Abdul Aziz, Syed Irfan Raza, Khadim Shah, Saad Ullah Khan, Muhammad Jawad Hassan, Wasim Ahmad
Turkish Journal of Biology
Genetic deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) due to mutations in the GALNS gene results in the pathogenesis of Morquio A syndrome. To date, more than 200 mutations have been reported in GALNS, resulting in variable clinical features. For this reason, bioinformatics analysis of these mutations is important to determine their effect on the structure and functions of the protein and to establish a correlation between genotype and phenotype. In the present study, eight Pakistani consanguineous families with Morquio A syndrome were clinically and genetically evaluated. Linkage analysis followed by sequence analysis of the gene detected four novel (p.Phe216Ser, p.Met38Arg, p.Ala291Ser, …
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
Turkish Journal of Biology
Tartary buckwheat is a type of coarse cereal that contains a rich supply of flavonoids. When suffering from certain stresses, tartary buckwheat can respond by promoting the biosynthesis of anthocyanins. It is well established that the jasmonate (JA) signaling pathway plays a critical role in plant resistance to disease by stimulating the production of anthocyanins; furthermore, the genes encoding the jasmonate-ZIM domain (JAZ) proteins have been shown to be key players in this process. However, the role of JAZ proteins in tartary buckwheat is poorly understood. In this study, we aimed to isolate FtJAZs from tartary buckwheat and identify FtJAZs …
Characterization Of Proteinase Inhibitor-Ii Gene Under Osrglp2promoter For Salt Stress In Transgenic Nicotiana Benthamiana, Shazia Rehman, Bodil Jørgensen, Soren Rasmussen, Tariq Mahmood
Characterization Of Proteinase Inhibitor-Ii Gene Under Osrglp2promoter For Salt Stress In Transgenic Nicotiana Benthamiana, Shazia Rehman, Bodil Jørgensen, Soren Rasmussen, Tariq Mahmood
Turkish Journal of Biology
Proteinase inhibitors in plants are mainly involved in conferring resistance against herbivores and pathogens. However, their role in abiotic stress resistance has also been reported. Therefore, transgenic tobacco plants were generated constitutively expressing the tomato proteinase inhibitor-II (PI-II) under control of the rice root germin-like protein 2 (OsRGLP2) promoter using Agrobacterium tumefaciens. T1 transgenic seedlings were subjected to stress treatments with different concentrations of sodium chloride (NaCl) (100 mM, 200 mM, 300 mM). Quantitative real-time PCR revealed considerable levels of expression and upregulation of PI-II in transgenic lines under salt stress. Considering the phenotypic effects, the transgenic seedlings showed better …
Analysis Of Ca125 Antigen In Normal Human Seminal Plasma Highlightsthe Molecular Heterogeneity Of Underlying Glycosylated Species, Ninoslav Mitic, Bojana Milutinovic, Miroslava Jankovic
Analysis Of Ca125 Antigen In Normal Human Seminal Plasma Highlightsthe Molecular Heterogeneity Of Underlying Glycosylated Species, Ninoslav Mitic, Bojana Milutinovic, Miroslava Jankovic
Turkish Journal of Biology
Cancer antigen CA125 represents an extracellular part of transmembrane mucin MUC16 and may be expressed in tissues of the male reproductive tract. It reaches human seminal plasma (hSP) after undergoing the main processes involved in protein speciation: synthesis, posttranslational modifications, compartmentalization, and auto/proteolytic degradation. This study was aimed at profiling CA125-immunoreactive species in hSP from healthy subjects as a specific and unexplored source of this tumor-associated antigen expressed under normal physiological conditions. High molecular mass components from total hSP and corresponding acid-soluble hSP preparations were analyzed. The results indicated that antibodies recognizing two distinct immunogenic areas of CA125 antigen exhibited …
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
Turkish Journal of Biology
This study reports the generation of a new human muscle tissue equivalent from skeletal muscle-derived stem cells and human umbilical vein endothelial cells (HUVECs). Skeletal muscle stem cells were isolated by the preplate technique and differentiated into neuron-like cells that were positive for neuronal beta-tubulin3 and nestin and negative for the astrocyte marker glial fibrillary acidic protein (GFAP). Coculture of skeletal muscle stem cells with the HUVECs under optimized fetal bovine serum and media conditions resulted in formation of a capillary network among the multinucleated myotubes. The neuron-like cells derived from the human skeletal muscle stem cells were seeded onto …
Role Of Snf5 In Rheumatoid Arthritis By Upregulation Ofp16 And Inactivation Of Jnk Pathway, Shupeng Wu, Jing Wang, Fang Li
Role Of Snf5 In Rheumatoid Arthritis By Upregulation Ofp16 And Inactivation Of Jnk Pathway, Shupeng Wu, Jing Wang, Fang Li
Turkish Journal of Biology
This study aims to explore the role and the possible underlying molecular mechanism of SNF5 in the pathogenesis of rheumatoid arthritis (RA). MH7A cells were respectively transfected with pc-SNF5 (pcDNA3.1 containing the Brg1 coding sequence), short hairpin RNA against SNF5 (shSNF5), and their negative controls (pcDNA3.1 and shNC). The alterations of SNF5 expression were assessed by qRT-PCR and western blot analysis. MTT assay, flow cytometry, and western blot analysis were performed to evaluate proliferation, apoptosis, and expression levels of p16 and JNK pathway associated proteins, respectively. Finally, the effect of SNF5 was verified in fibroblast-like synoviocytes (FLSs) obtained from a …
Characterization Of The Promoter Region Of The Glycerol-3-Phosphate-O-Acyltransferase Gene In Lilium Pensylvanicum, Lijing Chen, Li Zhang, Weikang Qi, Muhammad Irfan, Jing-Wei Lin, Hui Ma, Zhi-Fu Guo, Ming Zhong, Tian-Lai Li
Characterization Of The Promoter Region Of The Glycerol-3-Phosphate-O-Acyltransferase Gene In Lilium Pensylvanicum, Lijing Chen, Li Zhang, Weikang Qi, Muhammad Irfan, Jing-Wei Lin, Hui Ma, Zhi-Fu Guo, Ming Zhong, Tian-Lai Li
Turkish Journal of Biology
Cold environmental conditions influence the growth and development of plants, causing crop reduction or even plant death. Under stress conditions, cold-inducible promoters regulate cold-related gene expression as a molecular switch. Recent studies have shown that the chloroplast-expressed GPAT gene plays an important role in determining cold sensitivity. However, the mechanism of the transcriptional regulation of GPAT is ambiguous. The 5'-flanking region of GPAT with length of 1494 bp was successfully obtained by chromosome walking from Lilium pensylvanicum. The cis-elements of GPAT promoters were predicted and analyzed by a plant cis-acting regulatory DNA element database. There exist core promoter regions including …
Comparative Phytotoxicity Of Undoped And Er-Doped Zno Nanoparticles Onlemna Minor L.: Changes In Plant Physiological Responses, Samaneh Torbati, Alireza Khataee, Shabnam Saadi
Comparative Phytotoxicity Of Undoped And Er-Doped Zno Nanoparticles Onlemna Minor L.: Changes In Plant Physiological Responses, Samaneh Torbati, Alireza Khataee, Shabnam Saadi
Turkish Journal of Biology
The present study is the first research on the phytotoxicological effects of Er-doped zinc oxide nanoparticles (NPs) on duckweed (Lemna minor L.), as a model aquatic floating macrophyte. It concentrated on the comparison of the physiological effects of different concentrations of undoped and Er-doped zinc oxide NPs on L. minor. Pure and Er-doped zinc oxide samples were synthesized through a sonochemical method and their morphology and chemical composition were studied by scanning electron microscopy and energy dispersive X-ray analysis. Plant growth, photosynthetic pigment contents, and antioxidant enzyme activities were investigated as indices to assess the effects and toxicity of the …
The Effect Of Various Inducers And Their Combinations With Copper On Laccaseproduction Of Trametes Versicolor Pellets In A Repeated-Batch Process, Emre Bi̇rhanli, Özfer Yeşi̇lada
The Effect Of Various Inducers And Their Combinations With Copper On Laccaseproduction Of Trametes Versicolor Pellets In A Repeated-Batch Process, Emre Bi̇rhanli, Özfer Yeşi̇lada
Turkish Journal of Biology
The aim of this study was to increase laccase production in Trametes versicolor ATCC 200801 pellets by using various inducers and their combinations under repeated-batch conditions. Because copper is an effective inducer for laccase production, the effect of Cu on laccase production in this strain was tested first. The optimal Cu concentration for the highest laccase production was 0.5 mM. Following this preliminary study, the effect of other inducers [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), syringaldazine, guaiacol, and 2,5-xylidine] on laccase production was determined. Copper was determined to be the most efficient inducer among the inducers used. Therefore, the synergistic effect of each …
A R2r3 Myb Transcription Factor From Ash Positively Regulates Salt Response In Tobacco, Tian Li, Jingkuan Sun, Yuping Bi
A R2r3 Myb Transcription Factor From Ash Positively Regulates Salt Response In Tobacco, Tian Li, Jingkuan Sun, Yuping Bi
Turkish Journal of Biology
The roles of MYB transcription factors are diverse and important in regulating environmental stress in plants. In this study, the R2R3 MYB gene FvMYB2 (GenBank Accession No. KY767843) and its promoter region were cloned from ash (Fraxinus velutina Torr.). Phylogenetic analysis revealed that FvMYB2 was clustered with VvMYB60 from Vitis vinifera. PlantCARE software analysis showed that its promoter contained different cis elements involved in diverse abiotic stresses. The expression patterns of FvMYB2 were investigated under different abiotic stress conditions. FvMYB2 subcellular localization was mainly localized in the nucleus. Overexpression of FvMYB2 in tobacco showed enhanced salt resistance and upregulation of …
Optimization Of Monoclonal Antibody Expression In Chocells By Employing Epigenetic Gene Regulation Tools, Fatemeh Nematpour, Fereidoun Mahboudi, Vahid Khalaj, Behrouz Vaziri, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat, Fatemeh Davami
Optimization Of Monoclonal Antibody Expression In Chocells By Employing Epigenetic Gene Regulation Tools, Fatemeh Nematpour, Fereidoun Mahboudi, Vahid Khalaj, Behrouz Vaziri, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat, Fatemeh Davami
Turkish Journal of Biology
Monoclonal antibodies (mAbs) are crucial in pharmaceutical biotechnology. Mammalian cell lines are the most preferred for their production. One of the significant challenging issues of mammalian expression systems is epigenetic gene silencing. Employing epigenetic gene regulation tools can increase the productivity of the mammalian cell lines. Sodium butyrate (NaBut) and valproic acid (VPA) regulate gene expression by inhibiting histone deacetylase. A ubiquitous chromatin-opening element (UCOE) can improve expression by reducing DNA methylation. Here, the separate and combined effects of NaBut and VPA histone deacetylase inhibitors (iHDACs) and UCOE on mAb synthesis were studied. Stable cell lines were generated by non-UCOE …
Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler
Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler
Turkish Journal of Biology
Leaf rolling (LR), particularly in crops, is a dehydration avoidance mechanism that results from water loss in plants under drought conditions. Two maize cultivars (Zea mays L.) differing in their tolerance to drought (cultivar Side was selected as stress-tolerant and cultivar Karaçay was selected as stress-sensitive) under PEG-induced drought stress were used in this study to understand the effects of polyamines (PAs) on LR response and the role of the antioxidant system in the delayed LR process. Aerial parts of the seedlings were excised prior to the experiment and then submerged in a Hoagland nutrient solution with or without 0.1 …
Application Of Proline To Root Medium Is More Effective For Amelioration Of Photosynthetic Damages As Compared To Foliar Spraying Or Seed Soaking In Maize Seedlings Under Short-Term Drought, Mehmet Demi̇ralay, Cansu Altuntaş, Asi̇ye Sezgi̇n, Rabi̇ye Terzi̇, Asi̇m Kadioğlu
Application Of Proline To Root Medium Is More Effective For Amelioration Of Photosynthetic Damages As Compared To Foliar Spraying Or Seed Soaking In Maize Seedlings Under Short-Term Drought, Mehmet Demi̇ralay, Cansu Altuntaş, Asi̇ye Sezgi̇n, Rabi̇ye Terzi̇, Asi̇m Kadioğlu
Turkish Journal of Biology
Exogenous proline (PRO) at low concentrations can enhance drought stress tolerance in different application modes such as application to rooting medium, foliar spray, and seed soaking. However, there is no information about which application mode is more effective for increasing the drought tolerance. Comparative effects of 1, 10, and 20 mM PRO applications through three application modes to hydroponically grown seedlings were examined under short-term drought stress in maize seedlings. Effects on leaf water potential, membrane damage, chlorophyll content, proline level, and gas exchange parameters such as net photosynthetic rate (Pn), transpiration rate (E), stomatal conductance (gs), and substomatal CO2 …
Recovery And Amplification Of Ancient Dna From Herculaneum Victims Killed By The 79 Ad Vesuvius Hot Surges, Fabi̇o M. Guari̇no, Claudio Buccelli, Vincenzo Graziano, Pietro La Porta, Marcello Mezzasalma, Gaetano Odierna, Mariano Paternoster, Pierpaolo Petrone
Recovery And Amplification Of Ancient Dna From Herculaneum Victims Killed By The 79 Ad Vesuvius Hot Surges, Fabi̇o M. Guari̇no, Claudio Buccelli, Vincenzo Graziano, Pietro La Porta, Marcello Mezzasalma, Gaetano Odierna, Mariano Paternoster, Pierpaolo Petrone
Turkish Journal of Biology
n thermally damaged archaeological bones the quantity, quality, and amplifiability of DNA are very much reliant on both the extent of heating and the environmental conditions of the burial context. In this study we tested the possibility of extracting and amplifying ancient DNA from human bone remains of Herculaneum victims of the 79 AD eruption of Vesuvius using a combination of histochemical and molecular methods. Long bone samples with variable degrees of chromatic and morphological alterations consistent with exposure to temperatures of about 300 °C were taken from four specimens. Using histochemical stains, bone cryostat sections from three individuals revealed …
Ampicillin Activates Mpk1 Phosphorylation In Saccharomyces Cerevisiaeand Erk1/2 Phosphorylation In Hepg2 Cells, Yukyong Shin, Kiyoung Kim
Ampicillin Activates Mpk1 Phosphorylation In Saccharomyces Cerevisiaeand Erk1/2 Phosphorylation In Hepg2 Cells, Yukyong Shin, Kiyoung Kim
Turkish Journal of Biology
Ampicillin has been widely used to treat bacterial infections. When we used ampicillin to eliminate bacterial contamination in yeast cultures, we observed induction of phosphorylation of MAP kinase 1 (Mpk1), a previously unknown function of ampicillin. We therefore investigated whether ampicillin activates the signal transduction pathway. Phosphorylation of Saccharomyces cerevisiae Mpk1 was induced by ampicillin in a dose- and time-dependent manner through the PKC1-CWI pathway. Mpk1 phosphorylation was maximal after treatment with 3 mM ampicillin for 90 min. Despite activation of Mpk1 phosphorylation, ampicillin did not influence yeast cell growth. Ampicillin reduced miconazole antifungal activity; miconazole had a minimum inhibitory …
Aggregation Of A Parthenogenetic Diploid Embryo And A Male Embryo Improves The Blastocyst Development And Parthenogenetic Embryonic Stem Cell Derivation, Xiaoyan Qiu, Nan Li, Xiong Xiao, Yuemin Li
Aggregation Of A Parthenogenetic Diploid Embryo And A Male Embryo Improves The Blastocyst Development And Parthenogenetic Embryonic Stem Cell Derivation, Xiaoyan Qiu, Nan Li, Xiong Xiao, Yuemin Li
Turkish Journal of Biology
Parthenogenetically derived mammalian embryos, with no paternal genome, are not viable and die, largely from defective placental growth attributed to a lack of paternal effect, resulting in the low blastulation rate and low derivation efficiency of parthenogenetic embryonic stem cells (pESCs). Therefore, the present study, by the optimization of parthenogenetic embryo production and the aggregation of the parthenogenetic diploid embryo and the identified male embryo, aims to investigate a method to improve the development of parthenogenetic embryo and pESC derivation in mice. Using different chemical combinations for the optimization, we found that the heterozygous diploid type had a significantly higher …
Regulation Of Functional Activities And Essential Oil Production In Vetiveria Zizanioides L. Nash After γ-Irradiated Sodium Alginate Elicitation, Asfia Shabbir, Mohammad Masroor Akhtar Khan, Yawar Sadiq, Hassan Jaleel, Bilal Ahmad, Moin Uddin
Regulation Of Functional Activities And Essential Oil Production In Vetiveria Zizanioides L. Nash After γ-Irradiated Sodium Alginate Elicitation, Asfia Shabbir, Mohammad Masroor Akhtar Khan, Yawar Sadiq, Hassan Jaleel, Bilal Ahmad, Moin Uddin
Turkish Journal of Biology
Upon irradiation, marine polysaccharides undergo depolymerization, leading to formation of oligosaccharides that elicit various biological activities in plants. Taking a step further on the previously established growth-promoting activity of irradiated sodium alginate (ISA), structural rearrangements in ISA were analyzed using complementary techniques to develop an understanding of the structure-property relationship. The essential oil (EO) of vetiver (Vetiveria zizanioides L. Nash) immensely benefits the perfumery industry, proving itself as an economically important crop. A pot experiment was designed to test the effect of water-soluble ISA fractions on the growth, physiology, and EO production of vetiver. The structural characterization of radiation-induced sodium …
Silver Nanoparticles Induced Genotoxicity And Oxidative Stress In Tomato Plants, Fazi̇let Özlem Çeki̇ç, Sefa Eki̇nci̇, Müslüm Süleyman İnal, Di̇lek Özakça
Silver Nanoparticles Induced Genotoxicity And Oxidative Stress In Tomato Plants, Fazi̇let Özlem Çeki̇ç, Sefa Eki̇nci̇, Müslüm Süleyman İnal, Di̇lek Özakça
Turkish Journal of Biology
Among nanoparticles, silver nanoparticles (AgNPs) are intensively used in many materials owing to their antibacterial effects. In the present study different concentrations of AgNPs in Hoagland solution were applied to tomato seedlings. Total chlorophyll content, relative water content (RWC), antioxidant enzyme activities, and malondialdehyde content (MDA) as well as the genomic template stability (GTS) were analyzed. The intersimple sequence repeat polymerase chain reaction assay (ISSR-PCR) was used to determine the genotoxic effects of AgNPs on DNA. RWC did not change under AgNPs treatments; however, total chlorophyll content was significantly reduced by AgNPs applications. ISSR profiles demonstrated a consistent increase in …
Hybrid Nanomaterial: Biocolloidals, Numan Gözübenli̇, Emi̇r Yasun, Ni̇hat Di̇lsi̇z
Hybrid Nanomaterial: Biocolloidals, Numan Gözübenli̇, Emi̇r Yasun, Ni̇hat Di̇lsi̇z
Turkish Journal of Biology
Nanomaterials-based diagnostics have tremendous advantages over other conventional diagnostic systems in terms of sensitivity, specificity, and portability owing to the unique intrinsic properties of nanomaterials. Thus, there is a substantial need to develop and employ a broad spectrum of nanomaterials for biological and diagnostic applications. In this review, we focus on nanomaterials-assisted disease diagnosis utilizing different techniques such as photoluminescence, colorimetry, fluorescence, electrochemistry, microarray methods, surface plasmon resonance, and surface-enhanced Raman spectroscopy. In these techniques, different nanomaterials, including but not limited to gold and silver materials, metal oxides, and semiconductors, were employed according to their all-purpose chemical and physical properties. …
In Vitro Transcription And Validation Of Human Pancreatic Transcription Factors' Mrnas, Ersi̇n Akinci, Mehmet Yildiz, Peli̇n Ünal, Gamze Badakul
In Vitro Transcription And Validation Of Human Pancreatic Transcription Factors' Mrnas, Ersi̇n Akinci, Mehmet Yildiz, Peli̇n Ünal, Gamze Badakul
Turkish Journal of Biology
Direct reprogramming of pancreatic beta cells is of great interest due to its possible use in diabetes treatment. Various studies have demonstrated direct reprogramming of insulin expressing beta cells from other somatic cells by overexpressing important pancreatic transcription factors through viral vectors. However, concerns about using viral vectors in the clinic have prompted scientists to find new approaches for gene delivery and expression. In vitro synthesis and transfection of mRNAs is a safer strategy for ectopic gene expression. In this study we aimed to design and in vitro synthesize human pancreatic transcription factors' mRNAs: Pdx1, Ngn3, and MafA. We also …
In Vitro Evaluation Of Plla/Pbs Sponges As A Promisingbiodegradable Scaffold For Neural Tissue Engineering, İrem Ayşe Kanneci̇ Altinişik, Fatma Neşe Kök, Deni̇z Yücel, Gamze Köse
In Vitro Evaluation Of Plla/Pbs Sponges As A Promisingbiodegradable Scaffold For Neural Tissue Engineering, İrem Ayşe Kanneci̇ Altinişik, Fatma Neşe Kök, Deni̇z Yücel, Gamze Köse
Turkish Journal of Biology
In tissue engineering, the use of poly-L-lactic acid (PLLA)/polybutylene succinate (PBS) blend for the construction of scaffold is very limited. Moreover, polymeric sponges fabricated from PLLA/PBS have not been studied for neural tissue engineering. In the present study, the potential of the utility of PLLA/PBS polymeric sponges seeded with Schwann cells was investigated. PLLA and PBS were blended in order to increase the processability and tune the crystallinity, porosity, and degradation rate of the resulted polymeric sponges. These sponges were then seeded with Schwann cells. Porosity analysis showed that there were no significant differences between different compositions of PLLA/PBS blends; …
Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu
Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu
Turkish Journal of Biology
The yeast Pichia pastorisexpression system was investigated for the production of human cathepsin C (CatC) recombinant protein. The full-length CatC cDNA, corresponding to amino acids 12-475, was synthesized from interleukin-2 (IL-2) stimulated human peripheral blood mononuclear cells and subcloned in the pGEM-T cloning vector. After confirming the DNA sequence of the insert, the gene was cloned into the pPICZ\alphaA expression vector under the control of the methanol-inducible alcohol oxidase (AOX1) promoter and transformed to P. pastoris X-33 cells. The expressed protein was secreted into the culture medium through the alpha-factor mating signal sequence of the expression vector. Analysis of the …
Targeted Delivery Of Etoposide To Osteosarcoma Cells Using Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) (Phbv) Nanoparticles, Esma Alp, Tamer Çirak, Murat Demi̇rbi̇lek, Mustafa Türk, Eylem Güven
Targeted Delivery Of Etoposide To Osteosarcoma Cells Using Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) (Phbv) Nanoparticles, Esma Alp, Tamer Çirak, Murat Demi̇rbi̇lek, Mustafa Türk, Eylem Güven
Turkish Journal of Biology
Folic acid (FA)-functionalized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanoparticles were prepared to enhance the delivery efficiency of the anticancer drug etoposide for the clinical treatment of osteosarcoma. PHBV nanoparticles were synthesized by emulsification/solvent evaporation technique and obtained in the size range of 200-250 nm and zeta potential range of -21 and -27 mV. Encapsulation efficiency and in vitro drug release were studied. The cytotoxic, apoptotic, and necrotic effects of PHBV nanoparticles were also investigated using Saos-2 osteosarcoma cells. The results obtained in this study demonstrate that etoposide-loaded and FA-functionalized PHBV nanoparticles can be successfully used for targeted treatment of osteosarcoma.
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
Turkish Journal of Biology
In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial (HUVEC) and keratinocyte (HaCaT) cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and …