Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 10 of 10
Full-Text Articles in Life Sciences
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4- Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zeng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4- Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zeng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Articles
The top-performing linear poly(β-amino ester) (LPAE), poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (C32), has demonstrated gene transfection efficiency comparable to viral-mediated gene delivery. Herein, we report the synthesis of a series of highly branched poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (HC32) and explore how the branching structure influences the performance of C32 in gene transfection. HC32 were synthesized by an “A2 + B3 + C2” Michal addition strategy. Gaussia luciferase (Gluciferase) and green fluorescent protein (GFP) coding plasmid DNA were used as reporter genes and the gene transfection efficiency was evaluated in human cervical cancer cell line (HeLa) and human recessive dystrophic epidermolysis bullosa keratinocyte (RDEBK) cells. We …
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4-Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zheng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4-Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zheng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Articles
The top-performing linear poly(β-amino ester) (LPAE), poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (C32), has demonstrated gene transfection efficiency comparable to viral-mediated gene delivery. Herein, we report the synthesis of a series of highly branched poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (HC32) and explore how the branching structure influences the performance of C32 in gene transfection. HC32 were synthesized by an “A2 + B3 + C2” Michal addition strategy. Gaussia luciferase (Gluciferase) and green fluorescent protein (GFP) coding plasmid DNA were used as reporter genes and the gene transfection efficiency was evaluated in human cervical cancer cell line (HeLa) and human recessive dystrophic epidermolysis bullosa keratinocyte (RDEBK) cells. We …
Characterization Of Phytocystatin-Like Cysteine Protease Inhibitors Of Trichomonas Vaginalis, Ryan Michael John Faucher
Characterization Of Phytocystatin-Like Cysteine Protease Inhibitors Of Trichomonas Vaginalis, Ryan Michael John Faucher
University of the Pacific Theses and Dissertations
Trichomoniasis is a common STD caused by the parasitic protozoan Trichomonas vaginalis. The parasite is estimated to have infected roughly 3.7 million Americans. Complications from trichomoniasis can lead to cervical cancer in women and prostate cancer in men. One of the mechanisms of the parasite employs is using cysteine proteases to break down the cellular matrix of its host. However, three endogenous phytocystatin-like protease inhibitors have been found within the parasite’s genome. By recombinantly expressing these cystatins we have been able to test their ability to inhibit cysteine proteases such as papain and those found in T. vaginalis to find …
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Turkish Journal of Biology
The presence of uninhibited side effects of cancer drugs often used in cancer treatment has stimulated the search for alternative therapeutic approaches. Therefore, anticarcinogenic effects of synthetic, herbal, and fungal drugs have been investigated for the treatment of various cancer types in recent studies. Lichens, symbiotic organisms of fungi and algae, synthesize metabolites with significant biological activities. The aim of the current study was to screen the anticancer potential of usnic acid on various types of nonmalignant cell lines (Vero, L929) and cancer cell lines (CaCo2, RD, Hep2C, HepG2, Wehi). The growth inhibitory effect of usnic acid was determined by …
Pt(Iv), Pd(Ii), And Rh(Iii) Complexes Induced Oxidative Stress And Cytotoxicity In The Hct-116 Colon Cancer Cell Line, Petar Canovic, Jovana Bogojeski, Jelena Kosaric, Snezana Markovic, Marko Zivanovic
Pt(Iv), Pd(Ii), And Rh(Iii) Complexes Induced Oxidative Stress And Cytotoxicity In The Hct-116 Colon Cancer Cell Line, Petar Canovic, Jovana Bogojeski, Jelena Kosaric, Snezana Markovic, Marko Zivanovic
Turkish Journal of Biology
The use of transition metal complexes as appropriate analogues to cisplatin and similar anticancer drugs nowadays is of utmost importance. The inertness of Pt(IV), Pd(II), and Rh(III) complexes and the choosing of convenient ligands could offer a possible advantage in comparison to Pt(II) complexes. These advantages could be attributed to the better anticancer activity and fewer possible side effects. In this paper, we have chosen three complexes of different transition metal ions, [(TLtBu)PdIICl]ClO4, [RhIII(LH2tBu)Cl3], and [PtIV(LH2tBu)Cl3]Cl (where TLtBu is 2,6-bis[(1,3-di-tert-butylimidazolin-2-imino)methyl]pyridine and LH2tBu is 2,6-bis(5-tert-butyl-1H-pyrazol-3-yl)pyridine), for biological tests. All three complexes exerted strong prooxidative and cytotoxic effects on the human colorectal …
Wild-Growing Rosa Heckeliana Tratt.: Phenolic Constituentswith Cytotoxic And Antioxidative Properties, Nursen Çoruh, Ni̇zametti̇n Özdoğan
Wild-Growing Rosa Heckeliana Tratt.: Phenolic Constituentswith Cytotoxic And Antioxidative Properties, Nursen Çoruh, Ni̇zametti̇n Özdoğan
Turkish Journal of Biology
Rosa heckeliana, as one of the wild-growing species of the family Rosacea, has a wide use in the folk medicine; however, scientifically there is very little known about it. Our objective was to examine the antioxidative and antiproliferative properties of Rosa heckeliana root extract and its phenolics. The phenolic constituents, namely catechin, caffeic acid, and ellagic acid, were obtained by the methods of extraction, fractionation, and purification through column chromatography. The DPPH and ABTS radical scavenging assays and total flavonoid content analysis were employed as the current antioxidant methods over the crude extract and fractionated parts. Among the extracts/fractions, the …
Biocompatible Polymeric Coatings Do Not Inherently Reducethe Cytotoxicity Of Iron Oxide Nanoparticles, Ayli̇n Şendemi̇r Ürkmez, Ece Bayir, Eyüp Bi̇lgi̇, Mehmet Özgün Özen
Biocompatible Polymeric Coatings Do Not Inherently Reducethe Cytotoxicity Of Iron Oxide Nanoparticles, Ayli̇n Şendemi̇r Ürkmez, Ece Bayir, Eyüp Bi̇lgi̇, Mehmet Özgün Özen
Turkish Journal of Biology
Nanotechnology in biomedical research is an emerging and promising tool for different purposes, like high-resolution medical imaging, diagnostics, and targeted drug delivery. Although some experimental efforts focused on determination of the cytotoxicity of nanoparticles (NPs) are present, there are many controversial results. In this study, chitosan (CS)- and poly(acrylic acid) (PAA)-coated iron oxide nanoparticles (IONPs) were used to investigate the possible cytotoxicity of coated IONPs on model mammalian cell line SaOs-2 by evaluating cellular viability and membrane integrity. Increasing concentrations of IONPs increased the cytotoxic effect on SaOs-2 cells with both CS- and PAA-coated IONPs. Cell viability on day 3 …
Cytotoxic And Apoptotic Effects Of Endemic Centaurea Fenzlii Reichardt On Themcf-7 Breast Cancer Cell Line, Ümi̇t Yirtici, Fati̇h Göger, Mehmet Sarimahmut, Aysun Ergene
Cytotoxic And Apoptotic Effects Of Endemic Centaurea Fenzlii Reichardt On Themcf-7 Breast Cancer Cell Line, Ümi̇t Yirtici, Fati̇h Göger, Mehmet Sarimahmut, Aysun Ergene
Turkish Journal of Biology
The main purpose of this study was to analyze the cytotoxic activity of an extract obtained from Centaurea fenzlii Reichardt, and the fractions eluted from this extract, in breast cancer cells. After isolation and structural analysis of the fractions were conducted, a meaningful cytotoxic effect was indicated. The goal of the analysis was to reveal the mechanism by which this effect occurs through researching the apoptotic side of these fractions and determining the amount of several proteins that are the products of the genes. Test substances were applied to breast cancer cells and the inhibitory concentration value 50 (IC50) that …
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Turkish Journal of Biology
Recent studies have reported that cancer stem cells (CSCs) play a pivotal role in treatment failure, causing cancer recurrence. Here, we investigated the effects of L-NAME (an iNOS inhibitor) and celecoxib (a selective COX-2 inhibitor) on CSC-like cells (CSC-LCs) and their parental cells. Breast CSC-LCs derived from the MCF-7 cell line were sorted and characterized with the CD44+/CD24-/low phenotype. After isolation, the percentage of the subpopulation expressing CD44+/CD24-/low biomarkers increased considerably from 0.96% to 28.6%. Use of L-NAME and celecoxib showed antiproliferative activity towards both MCF-7 and CSC-LCs. Although celecoxib enhanced apoptotic cell death, the CSC-LC population was more resistant …
The Mtt Viability Assay Yields Strikingly False-Positive Viabilities Although The Cells Are Killed By Some Plant Extracts, Di̇dem Karakaş, Ferda Ari, Engi̇n Ulukaya
The Mtt Viability Assay Yields Strikingly False-Positive Viabilities Although The Cells Are Killed By Some Plant Extracts, Di̇dem Karakaş, Ferda Ari, Engi̇n Ulukaya
Turkish Journal of Biology
The MTT assay is one of the often used cell viability/cytotoxicity assays. However, when the methanol extracts of plants are used to test their cytotoxic potential, interference may occur, resulting in false-positive viability results. Therefore, in this study, the reliability of the MTT assay was investigated in the case of plant use. The methanol extracts of three different plants (Hypericum adenotrichum, Salvia kronenburgii, and Pelargonium quercetorum) were tested in breast cancer cell lines (MCF-7 and MDA-MB-231) using the MTT assay and the results were compared to the ATP assay, which is a much more sensitive and reliable assay due to …