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Full-Text Articles in Life Sciences
Regulation Of Learning And Memory By The Drosophila Melanogaster Swi/Snf Complex, Max H. Stone
Regulation Of Learning And Memory By The Drosophila Melanogaster Swi/Snf Complex, Max H. Stone
Electronic Thesis and Dissertation Repository
The SWI/SNF complex is a highly-conserved ATP-dependent chromatin remodelling complex that is important in the etiology of intellectual disability (ID). I systematically investigated the overall and adult-specific roles of each of the 15 Drosophila melanogaster SWI/SNF complex components in memory. Flies with RNAi-mediated knockdown of individual SWI/SNF genes in the mushroom body (MB) were tested for short- and long-term memory impairment using courtship conditioning. Knockdown of several SWI/SNF genes through development, including brahma, Bap60, Snr1, and e(y)3, caused loss of memory. Adult-specific knockdown of SWI/SNF genes caused some loss of memory phenotypes, indicating an acute role in adult MB activity. …
Gene Expression Profiling In The Larval Fat Body Of Desiccation Selected Drosophila Melanogaster, Adriana Michelle Charles
Gene Expression Profiling In The Larval Fat Body Of Desiccation Selected Drosophila Melanogaster, Adriana Michelle Charles
UNLV Theses, Dissertations, Professional Papers, and Capstones
Drosophila melanogaster selected for resistance to desiccation (no food or water) display slower development and a higher body mass compared to fed controls due to an extended third larval instar. I hypothesize that desiccation selected D. melanogaster larvae will have a different gene expression profile compared to fed controls. Separate populations of D. melanogaster were subjected to desiccation (no food or water), starvation (no food) until 80-85% mortality, for 75 generations. mRNA from the larval fat body was collected at 88 hours, 96 hours, 112 hours and 120 hours post-hatching. Four replicate samples were used for each condition and time …
In Vivo Characterization Of The Drosophila Mrna 3’-End Processing Core Cleavage Complex: Poly(A) Mrna & Histone Mrna 3’-End Processing, Daniel Michalski
In Vivo Characterization Of The Drosophila Mrna 3’-End Processing Core Cleavage Complex: Poly(A) Mrna & Histone Mrna 3’-End Processing, Daniel Michalski
Dissertations
A core cleavage complex (CCC) consisting of CPSF73, CPSF100 and Symplekin is required for co-transcriptional 3’ end processing of all metazoan pre-mRNAs, yet little is known about the in vivo molecular interactions within this complex. The CCC is a component of two distinct complexes, the cleavage/polyadenylation complex and the complex that processes non-polyadenylated histone pre-mRNAs. RNAi-depletion of CCC factors in Drosophila culture cells causes reduction of CCC processing activity on histone mRNAs, resulting in read through transcription. In contrast, RNAi-depletion of factors only required for histone mRNA processing allows use of downstream cryptic polyadenylation signals to produce polyadenylated histone mRNAs. …
Blue-Light Induced Accumulation Of Reactive Oxygen Species Is A Consequence Of The Drosophila Cryptochrome Photocycle, Louis David Arthaut, Margaret Ahmad, Nathalie Jourdan, Ali Mteyrek, Maria Procopio, Mohamed A. El-Esawi, Alain D'Harlingue, Pierre Etienne Bouchet, Jacques Witczak, Carlos F. Martino
Blue-Light Induced Accumulation Of Reactive Oxygen Species Is A Consequence Of The Drosophila Cryptochrome Photocycle, Louis David Arthaut, Margaret Ahmad, Nathalie Jourdan, Ali Mteyrek, Maria Procopio, Mohamed A. El-Esawi, Alain D'Harlingue, Pierre Etienne Bouchet, Jacques Witczak, Carlos F. Martino
Biomedical Engineering and Sciences Faculty Publications
Cryptochromes are evolutionarily conserved blue-light absorbing flavoproteins which participate in many important cellular processes including in entrainment of the circadian clock in plants, Drosophila and humans. Drosophila melanogaster cryptochrome (DmCry) absorbs light through a flavin (FAD) cofactor that undergoes photoreduction to the anionic radical (FAD) redox state both in vitro and in vivo. However, recent efforts to link this photoconversion to the initiation of a biological response have remained controversial. Here, we show by kinetic modeling of the DmCry photocycle that the fluence dependence, quantum yield, and half-life of flavin redox state interconversion are consistent with the anionic radical (FAD∗-) …
The Effect Of Age On Social Behaviour In Drosophila Melanogaster And The Progeny Of Aged Parents, Dova Brenman
The Effect Of Age On Social Behaviour In Drosophila Melanogaster And The Progeny Of Aged Parents, Dova Brenman
Electronic Thesis and Dissertation Repository
Recent studies have linked neuropsychiatric disorders to older parents. These disorders often include changes in social behaviours like the social spacing between neighbouring individuals, which can be modeled in organisms such as Drosophila melanogaster. I investigated the effects of aging on the social space between neighbouring D. melanogaster and how aging impacts the next generation. To achieve this, I used the social space assay and found that individuals become less social with age and that this effect is passed on to the first generation only. Additionally, accelerating the physiological process of aging via increased rearing temperatures or exposure to …
Does Cold Activate The Drosophila Melanogaster Immune System?, Golnaz Salehipour-Shirazi, Laura V Ferguson, Brent J Sinclair
Does Cold Activate The Drosophila Melanogaster Immune System?, Golnaz Salehipour-Shirazi, Laura V Ferguson, Brent J Sinclair
Biology Publications
Cold exposure appears to activate aspects of the insect immune system; however, the functional significance of the relationship between cold and immunity is unclear. Insect success at low temperatures is shaped in part by interactions with biotic stressors, such as pathogens, thus it is important to understand how and why immunity might be activated by cold. Here we explore which components of the immune system are activated, and whether those components differ among different kinds of cold exposure. We exposed Drosophila melanogaster to both acute (2h, -2°C) and sustained (10h, -0.5°C) cold, and measured potential (antimicrobial peptide expression, phenoloxidase activity, …
Real-Time Quantitative Pcr To Demonstrate Gene Expression In An Undergraduate Lab, Abijeet Singh Mehta, Amit Singh
Real-Time Quantitative Pcr To Demonstrate Gene Expression In An Undergraduate Lab, Abijeet Singh Mehta, Amit Singh
Biology Faculty Publications
The objective of this teaching note is to develop a laboratory exercise, which allows students to get a hands-on experience of a molecular biology technique to analyze gene expression. The short duration of the biology laboratory for an undergraduate curriculum is the biggest challenge with the development of new labs. An important part of cell biology or molecular biology undergraduate curriculum is to study gene expression. There are many labs to study gene expression in qualitative manner. The commonly used reporter gene expression studies are primarily qualitative. However, there is no hands-on experience exercise to quantitatively determine gene expression. Therefore, …
Exploring The Interaction Of Drosophila Tdp-43 And The Type Ii Voltage-Gated Calcium Channel, Cacophony, In Regulating Motor Function And Behavior, Kayly M. Lembke, David B. Morton
Exploring The Interaction Of Drosophila Tdp-43 And The Type Ii Voltage-Gated Calcium Channel, Cacophony, In Regulating Motor Function And Behavior, Kayly M. Lembke, David B. Morton
Faculty Publications - Department of Biological & Molecular Science
Amyotrophic lateral sclerosis (ALS) is the most common adult onset motor neurodegenerative disease. The cause of the disease remains obscure, and as such there is no effective treatment or cure. Amyotrophic lateral sclerosis and other neurodegenerative diseases are frequently characterized by dysfunction of the RNA-binding protein, TDP-43. Using model systems to understand the mechanisms underlying TDP-43 dysfunction should accelerate identification of therapeutic targets. A recent report has shown that motor defects caused by the deletion of the Drosophila TDP-43 ortholog, tbph, are not driven by changes in the physiology at the neuromuscular junction. Rather, defective motor burst rhythmicity and coordination, …
An Undergraduate Cell Biology Lab: Western Blotting To Detect Proteins From Drosophila Eye, Neha Gogia, Ankita Sarkar, Amit Singh
An Undergraduate Cell Biology Lab: Western Blotting To Detect Proteins From Drosophila Eye, Neha Gogia, Ankita Sarkar, Amit Singh
Biology Faculty Publications
We have developed an undergraduate laboratory to allow detection and localization of proteins in the compound eye of Drosophila melanogaster, a.k.a fruit fly. This lab was a part of the undergraduate curriculum of the cell biology laboratory course aimed to demonstrate the use of Western Blotting technique to study protein localization in the adult eye of Drosophila. Western blotting, a two-day laboratory exercise, can be used to detect the presence of proteins of interests from total protein isolated from a tissue. The first day involves isolation of proteins from the tissue and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide) gel …
Lipocalin Gene Expression Is Varied In Developmental Stages By Larval Nutritional Stress In Drosophila, Nazli Ayhan, Pinar Güler, Banu Şebnem Önder
Lipocalin Gene Expression Is Varied In Developmental Stages By Larval Nutritional Stress In Drosophila, Nazli Ayhan, Pinar Güler, Banu Şebnem Önder
Turkish Journal of Biology
Lipocalin genes NLaz, GLaz, and Karl are evolutionarily conserved genes in Drosophila melanogaster. There are studies on lipocalin gene expression differences under diverse diet conditions, but these studies have focused mainly on age-dependent expression profiles of these genes. The main aim of our study is to determine lipocalin expression in the developmental period by nutritional manipulation with an isofemale-based design. Three larval developmental periods have been researched under normal and restricted diets. We found significant differences between lines during their developmental time-related lipocalin expression. Here, we demonstrate that upregulations in the early developmental stages of lipocalin genes under stressful conditions …