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The Contribution Of Active Efflux In Reduced Susceptibilities To Copper Sulfate And Zinc Chloride In Escherichia Coli Isolates From Swine, Chailai Koowatananukul, Nisit Chansong, Rungtip Chuanchuen 2010 Chulalongkorn University

The Contribution Of Active Efflux In Reduced Susceptibilities To Copper Sulfate And Zinc Chloride In Escherichia Coli Isolates From Swine, Chailai Koowatananukul, Nisit Chansong, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

A hundred-eighty Escherichia coli isolates from swine were tested for minimal inhibitory concentrations (MICs) of 9 antimicrobials and two heavy metals copper sulfate and zinc chloride. All the E. coli isolates were resistant to at least one antibiotic and 175 (97.2%) isolates were multi-drug resistant. Forty antibiotic resistance patterns were identified, of which the most common pattern was AMP-CHP-CIP-ERY-GEN-STR-SUL-TET-TRI (13.3%). All the isolates formed one large population of susceptibility to copper sulfate and zinc chloride at the concentrations of 6.4-12.8 mM and 3.2-6.4 mM, respectively. Exposure to copper sulfate and zinc chloride at sublethal concentration did not change the susceptibility. …


Blood Pressure, Urinary Protein Creatinine Ratio And Oxidative Stress In Dogs With Urolithiasis, Supa Sithanukul, Poramin Shayarattanasin, Vimolpa Hiranpradith, Winai Chansaisakorn, Monkon Trisiriroj, Siripen Komolvanich, Saikaew Satayatham, Chollada Buranakarl 2010 Chulalongkorn University

Blood Pressure, Urinary Protein Creatinine Ratio And Oxidative Stress In Dogs With Urolithiasis, Supa Sithanukul, Poramin Shayarattanasin, Vimolpa Hiranpradith, Winai Chansaisakorn, Monkon Trisiriroj, Siripen Komolvanich, Saikaew Satayatham, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

Blood pressure, urinary protein creatinine (UPC) ratio and oxidative stress (plasma melondialdehyde, urinary melondialdehyde creatinine ratio; UMDA/Cr, the activities of red blood cell catalase; RBC-CAT and red blood cell glutathione) were studied in 42 dogs with urolithiasis and 14 control healthy dogs. The types and location of calculi were defined. The results showed that calculi preferentially found in male and female dogs were calcium oxalate and struvite, respectively. In female, calculi found mostly at urinary bladder while in male, they could be found in either urinary bladder and/or urethra. Dogs with urolithiasis were normotensive. However, UPC ratio was higher significantly …


Evaluation Of Serum Nt-Probnp Levels In Dogs With Respiratory Distress Associated With Heart Disease And Respiratory Disease, Amornrate Sastravaha, Santi Kaewmokul, Pareeya Udomkusonsri, Naris Thengchaisri 2010 Chulalongkorn University

Evaluation Of Serum Nt-Probnp Levels In Dogs With Respiratory Distress Associated With Heart Disease And Respiratory Disease, Amornrate Sastravaha, Santi Kaewmokul, Pareeya Udomkusonsri, Naris Thengchaisri

The Thai Journal of Veterinary Medicine

The association between severity of heart disease and serum N-terminal prohormone B-type natriuretic peptide (NT-proBNP) was determined in dogs presented with respiratory distress. Four groups of dogs were divided into healthy control (n=11), heart failure (n=58), heart disease without heart failure (n=17) and respiratory disease (n=20). Serum NT-proBNP concentrations were measured with an ELISA designed for using in dogs. Dirofilaria antigen tests were detected in dogs with heart disease or heart failure. Serum NT-proBNP concentrations of dogs with heart failure (2,977±184 pmol/l) were significantly higher than dogs with heart disease (611±46 pmol/l), respiratory disease (583±52 pmol/l) or physically healthy (426±32 …


Detection Of Necrotizing Hepatopancreatitis (Nhp) In Wild Shrimp From Laguna Madre, Mexico By A Multiplex Polymerase Chain Reaction, Gabriel Aguirre-Guzman, Jesus Genaro Sanchez-Martinez, Roberto Pérez-Castañeda, Rafael Orta-Rodriguez 2010 Chulalongkorn University

Detection Of Necrotizing Hepatopancreatitis (Nhp) In Wild Shrimp From Laguna Madre, Mexico By A Multiplex Polymerase Chain Reaction, Gabriel Aguirre-Guzman, Jesus Genaro Sanchez-Martinez, Roberto Pérez-Castañeda, Rafael Orta-Rodriguez

The Thai Journal of Veterinary Medicine

The Laguna Madre in Mexico is a nursery, growth, and refuge area for shrimp and other wildlife species. These organisms support an important fishery for local industry (third place in Mexico), which may suffer diseases that are few studied. Wild shrimp were randomly collected from two sampling stations (Carrizal and Carbonera) once a month for one year (September 2007 to September 2008) to screen for virus and bacteria. The shrimp were taxonomically identified, organized in pools of three organisms per species and analyzed for the presence of WSSV, IHHNV, NHP and HPV by a commercial multiplex PCR kit DiagXotics Inc. …


Ecg Quiz, Chollada Buranakarl, Winai Chansaisakorn 2010 Chulalongkorn University

Ecg Quiz, Chollada Buranakarl, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.


Ophthalmology Snapshot, Nalinee Tuntivanich 2010 Chulalongkorn University

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


Comparative Study On Six Different Long Term Commercial Extenders For Fresh Boar Semen, Kampon Kaeoket, Titisa Srisowanna, Ubonrat Wichaidit, Panida Chanapiwat, Sukanya Manee-in 2010 Chulalongkorn University

Comparative Study On Six Different Long Term Commercial Extenders For Fresh Boar Semen, Kampon Kaeoket, Titisa Srisowanna, Ubonrat Wichaidit, Panida Chanapiwat, Sukanya Manee-In

The Thai Journal of Veterinary Medicine

In Thailand, there are numerous commercial extenders for preservation of boar semen; however, no study has been carried out to compare these extenders in view of maintaining of sperm motility, plasma membrane integrity and acrosome integrity during cold storage. The aim of this study was to assess the percentage of sperm motility, HOST positive, viability and acrosome integrity of boar spermatozoa extended in Merck-III, Androstar®Plus, ModenaTM, NUTRIXcell®, VITASEM LD, Duragen and Dofu goldTM. Ejaculated from boars (n=6, one ejaculate from each boar) were collected and sub-samples (splited samples) were diluted (3x109 spermatozoa/100 ml) in the different extenders and stored for …


Sano (Sesbania Javanica Miq.) Flower As A Pigment Source In Egg Yolk Of Laying Hens, Suwanna Kijparkorn, Hatairat Plaimast, Somporn Wangsoonoen 2010 Chulalongkorn University

Sano (Sesbania Javanica Miq.) Flower As A Pigment Source In Egg Yolk Of Laying Hens, Suwanna Kijparkorn, Hatairat Plaimast, Somporn Wangsoonoen

The Thai Journal of Veterinary Medicine

The study was conducted to investigate Sano flower that naturally contains high concentration of yellow fat soluble pigment as a pigment source for egg yolk in layer diet. Dried Sano flower (Sano) was prepared and analyzed for nutritional composition and total carotenoids content. Six dietary treatments were: 1) corn-soy basal diet that contain total carotenoids from corn at 12 mg/kg diet, 2)-4) Sano diet that contain total carotenoids from Sano at 10, 15 and 30 mg/kg diet, 5) mixed carotenoids diet that contain total carotenoids from Sano at 10 mg/kg and synthetic pigment, canthaxanthin at 2 mg/kg diet, and 6) …


Forensic Veterinary Medicine, Nantarika Chansue 2010 Chulalongkorn University

Forensic Veterinary Medicine, Nantarika Chansue

The Thai Journal of Veterinary Medicine

No abstract provided.


Establishment Of Porcine Embryonic Stem-Like Cells From Parthenogenetic And In Vivo Derived Embryos, Sasithorn Panasophonkul, Theerawat Tharasanit, Mongkol Techakumphu 2010 Chulalongkorn University

Establishment Of Porcine Embryonic Stem-Like Cells From Parthenogenetic And In Vivo Derived Embryos, Sasithorn Panasophonkul, Theerawat Tharasanit, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The study was conducted to examine the effect of different parthenogenetic activation (PA) protocols on developmental competence and quality of embryos, and to establish the parthenogenetic embryonic stem (ptES)-like cells in pigs. Compacted cumulus-oocyte complexes (COCs) were collected from porcine ovaries and then cultured in M199-medium. After 44 hrs of culture, matured MII-oocytes were selected and divided into three groups. Oocytes in each group were stimulated with three different activation protocols as protocol I: 1.36 kV/cm, 30 μsec, 2 pulses; protocol II: 1.50 kV/cm, 60 μsec, 2 pulses and protocol III: 1.0 kV/cm, 80 μsec, 3 pulses, and followed by …


Comparison Of The Efficacy Of Enrofloxacin Against Escherichia Coli Or Pasteurella Multocida Infection In Chickens, Visut Rawiwet, Piyarat Chansiripornchai, Niwat Chansiripornchai 2010 Chulalongkorn University

Comparison Of The Efficacy Of Enrofloxacin Against Escherichia Coli Or Pasteurella Multocida Infection In Chickens, Visut Rawiwet, Piyarat Chansiripornchai, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

This study compared the efficacy of enrofloxacin, given immediately or 6 hrs after infection against Escherichia coli or Pasteurella multocida. In the first experiment, thirty, 12-week-old male layer chickens were divided into 3 groups of 10 each. Chickens in group 1, 2 and 3 were infected with E. coli serotype O78 by left thoracic air sac injection. Group 1 was given enrofloxacin (10 mg/kg), dissolved in drinking water immediately receiving E. coli. Group 2 was given enrofloxacin 6 hrs after receiving E. coli. Group 3 served as a positive control. In the second experiment, twenty, 16-week-old male layer chickens were …


Prevalence Of The Cellular And Molecular Antimicrobial Resistance Against E. Coli Isolated From Thai Broilers, Sarawoot Mooljuntee, Piyarat Chansiripornchai, Niwat Chansiripornchai 2010 Chulalongkorn University

Prevalence Of The Cellular And Molecular Antimicrobial Resistance Against E. Coli Isolated From Thai Broilers, Sarawoot Mooljuntee, Piyarat Chansiripornchai, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The antimicrobial resistant characteristics both of the cellular and molecular levels of Escherichia coli in broilers were performed by disk diffusion test and polymerase chain reaction technique. Specific primers for the aadA, aac(3)-IV, cmlA, cat1, tetA, sul1, SHV, CITM, ereA and dhfrV gene were used to detect resistance to gentamicin, chloramphenicol, tetracycline, cephalothin, ampicillin, erythromycin, sulfonamide+trimetroprim. The 30 samples of E. coli were found to be 100% antimicrobial disk resistant to tetracycline, ampicillin and erythromycin and revealed a 90%, 93.3% and 73.3% resistant gene to tetA, CITM and ereA, respectively. While resistance to 73.3% cephalothin and 26.7% sulfonamide+trimethoprim was revealed …


Morphological, Cytochemical And Ultrastructural Studies Of Blood Cells In Irrawaddy River Dolphin (Orcaella Brevirostris): A Case Study, Somporn Techangamsuwan, Supradit Wangnaithum, Jariya Sutanonpaiboon, Nantarika Chansue, Achariya Sailasuta 2010 Chulalongkorn University

Morphological, Cytochemical And Ultrastructural Studies Of Blood Cells In Irrawaddy River Dolphin (Orcaella Brevirostris): A Case Study, Somporn Techangamsuwan, Supradit Wangnaithum, Jariya Sutanonpaiboon, Nantarika Chansue, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

The characteristic of erythrocytes, leukocytes and thrombocytes of a captive Irrawaddy river dolphin (Orcaella brevirostris) was described using blood sample collected from caudal vein. By morphological and ultrastructural studies, erythrocytes showed similar size as in humans and contained cytoplasmic electron dense hemoglobin without nucleus. Multilobulated granulocytes composed of cytoplasmic round-to-elongated granulecontaining neutrophils and round-to-oval shaped granule-bearing eosinophils while basophil was very scanty. Monocytes displayed azulophilic granules and vacuolated cytoplasm with pseudopodia. Lymphocytes showed equal amounts of heterochromatin and euchromatin with numerous mitochondria in their cytoplasm. Thrombocytes, the smallest circulating blood cells, presented small pseudopodia with many cytoplasmic microtubular structures. By …


Isolation Of Actinobacillus Pleuropneumoniae Serotype 15-Like Strain From A Porcine Tonsil In Thailand: A Case Report, Walaiporn Tonpitak 2010 Chulalongkorn University

Isolation Of Actinobacillus Pleuropneumoniae Serotype 15-Like Strain From A Porcine Tonsil In Thailand: A Case Report, Walaiporn Tonpitak

The Thai Journal of Veterinary Medicine

An isolate of Actinobacillus (A.) pleuropneumoniae from porcine tonsil samples was identified as serotype 15-like strain, which was previously isolated in Australia, North America and Japan. It was characterized by biochemical testing performed for Actinobacillus sp. and confirmed as A. pleuropneumoniae by PCR and nucleotide sequencing of 16s rDNA. The isolate contains apxIBD, apxIICA, apxIIIBD, apxIIICA and apxIVA gene. The result of PCR-REA of apxIVA method was identical as A. pleuropneumoniae serotype 15 strain HS143 reference strain. Therefore this isolate is proposed as A. pleuropneumoniae serotype 15- like strain and the first report of A. pleuropneumoniae serotype 15-like strain in …


Ultrasound Diagnosis, Phiwipha Kamonrat 2010 Chulalongkorn University

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph 2010 Chulalongkorn University

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.


Animal Health Matters, Jarett Bies, David H. Zeman, Russ Daly 2010 South Dakota State University

Animal Health Matters, Jarett Bies, David H. Zeman, Russ Daly

Animal Health MATTERS Newsletter

[Page] 1- New Department Name Introduced in Campus Ceremony [Page] 1- Director's Message: What’s in a Name? [Page] 2- VBS Department Faculty Make Connections in China [Page] 3- ADRDL Personnel Gain Additional Proficiency Training for Foreign Animal Disease Testing, Embark on Test Validation Studies [Page] 3- Veterinary & Biomedical Sciences Department Launches New Website: www.sdstate.edu/vs [Page] 4- Histopathology and the Histology Laboratory at SDSU’s ADRDL Pieces and Parts [Page] 6- Dunn Begins Work as Dean of SDSU College of Agriculture & Biological Sciences [Page] 6- Hennings to Address International Pig Veterinary Society [Page] 6- SDSU Faculty to Co-Author “Diseases of …


Pemoline And Tetramisole 'Positives' In English Racehorses Following Levamisole Administration, J. Gutierrez, R. L. Eisenberg, N. J. Koval, E. R. Armstrong, J. Tharappel, C. J. Hughes, Thomas Tobin 2010 University of Kentucky

Pemoline And Tetramisole 'Positives' In English Racehorses Following Levamisole Administration, J. Gutierrez, R. L. Eisenberg, N. J. Koval, E. R. Armstrong, J. Tharappel, C. J. Hughes, Thomas Tobin

Veterinary Science Faculty Publications

Pemoline is a central nervous system stimulant that has been used to treat attention-deficit hyperactivity disorder and narcolepsy in humans; its identification in horses could be considered evidence of attempts to influence performance. Two recent pemoline 'positives' in English racehorses led us to review the chemical relationships between tetramisole, levamisole, aminorex and pemoline. Pemoline is a simple oxidation product of aminorex, which has been shown in the United States and elsewhere to be an equine metabolite of levamisole. Based on the clear structural relationships between aminorex and pemoline, we conclude that levamisole can metabolise to pemoline in horses and that …


The Glycoproteins Of Porcine Reproductive And Respiratory Syndrome Virus And Their Role In Infection And Immunity, Phani B. Das 2010 University of Nebraska-Lincoln

The Glycoproteins Of Porcine Reproductive And Respiratory Syndrome Virus And Their Role In Infection And Immunity, Phani B. Das

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important pathogen of swine and is known to cause abortion and infertility in pregnant sows and respiratory distress in piglets. PRRSV contains a major glycoprotein (GP5) and three minor glycoproteins (GP2a, GP3, and GP4) on the virion envelope, all of which are required for infectious virus production. To study their interactions amongst each other and with a cellular receptor for PRRSV, CD163, I cloned each of the viral glycoproteins and CD163 in various expression vectors. My studies have shown that while the GP2a, GP3, and GP4 are co-translationally glycosylated, …


Human Induced Rotation And Reorganization Of The Brain Of Domestic Dogs, Taryn Roberts, Paul McGreevy, Michael Valenzuela 2010 University of Sydney

Human Induced Rotation And Reorganization Of The Brain Of Domestic Dogs, Taryn Roberts, Paul Mcgreevy, Michael Valenzuela

Anatomy Collection

Domestic dogs exhibit an extraordinary degree of morphological diversity. Such breed-to-breed variability applies equally to the canine skull, however little is known about whether this translates to systematic differences in cerebral organization. By looking at the paramedian sagittal magnetic resonance image slice of canine brains across a range of animals with different skull shapes (N = 13), we found that the relative reduction in skull length compared to width (measured by Cephalic Index) was significantly correlated to a progressive ventral pitching of the primary longitudinal brain axis (r = 0.83), as well as with a ventral shift in the position …


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