Cloned Asian Elephant (Elephas Maximus) Embryos Reconstructed From Rabbit Recipient Oocytes,
2010
Chulalongkorn University
Cloned Asian Elephant (Elephas Maximus) Embryos Reconstructed From Rabbit Recipient Oocytes, Mongkol Techakumphu, Ruttachuk Rungsiwiwut, Pranee Numchaisrika, Ampika Thongphakdee
The Thai Journal of Veterinary Medicine
The information about reproductive biology, especially embryo development of Asian elephant (Elephas maximus) generated from naturally fertilization is lacking. In the present study, somatic cell nuclear transfer (SCNT) was applied as an alternative way to produce the elephant embryos. The fibroblasts derived from ear skin of Asian elephant and rabbit were used as the donor cells and rabbit oocytes were used as the recipient cytoplasm. The objectives of the study were 1) to find the optimal conditions for fusion and activation by using electrical pulses (experiment I) and 2) to investigate the in vitro development of cloned Asian elephant in …
The 40th Anniversary Of The Thai Journal Of Veterinary Medicine,
2010
Chulalongkorn University
The 40th Anniversary Of The Thai Journal Of Veterinary Medicine, Songkram Luangtongkum
The Thai Journal of Veterinary Medicine
No abstract provided.
Ophthalmology Snapshot,
2010
Chulalongkorn University
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
What Is Your Diagnosis,
2010
Chulalongkorn University
What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep
The Thai Journal of Veterinary Medicine
No abstract provided.
Cell-Type Specific Distribution Of Chloride Transporters In The Rat
Suprachiasmatic Nucleus,
2010
Hebrew University of Jerusalem
Cell-Type Specific Distribution Of Chloride Transporters In The Rat Suprachiasmatic Nucleus, Michael A. Belenky, Patricia J. Sollars, David B. Mount, Seth L. Alper, Yosef Yarom, Gary E. Pickard
School of Veterinary and Biomedical Sciences: Faculty Publications
The suprachiasmatic nucleus (SCN) is a circadian oscillator and biological clock. Cell-to-cell communication is important for synchronization among SCN neuronal oscillators and the great majority of SCN neurons use γ-aminobutyric acid (GABA) as a neurotransmitter, the principal inhibitory neurotransmitter in the adult central nervous system. Acting via the ionotropic GABAA receptor, a chloride ion channel, GABA typically evokes inhibitory responses in neurons via Cl− influx. Within the SCN GABA evokes both inhibitory and excitatory responses although the mechanism underlying GABA-evoked excitation in the SCN is unknown. GABA-evoked depolarization in immature neurons in several regions of the brain is a function …
Hair Whorls In The Dog (Canisfamiliaris). I. Distribution,
2010
University of Sydney
Hair Whorls In The Dog (Canisfamiliaris). I. Distribution, Lisa M. Tomkins, Paul Mcgreevy
Anatomy Collection
Hair whorl characteristics were assessed in the domestic dog (Canis familiaris) in the regions of cephalic, cervical (dorsal, ventral, and lateral), thoracic and brachial axillary regions, the chest, shoulders, elbows, ventral abdominal region, and on the caudal thighs (ischiatic). They were classified as simple or tufted, and their position was recorded as the distance between their centers and bony landmarks within each region. The distribution of whorls was explored in a cohort of domestic dogs (N = 120) comprising a variety of breeds and cross‐breeds, sourced from shelters (N = 60) and the general public (N = 60). …
The Duration Of The Effects Of Repeated Widespread Badger Culling On Cattle Tuberculosis Following The Cessation Of Culling,
2010
Imperial College London
The Duration Of The Effects Of Repeated Widespread Badger Culling On Cattle Tuberculosis Following The Cessation Of Culling, Helen E. Jenkins, Rosie Woodroffe, Christl A. Donnelly
Michigan Bovine Tuberculosis Bibliography and Database
Background: In the British Isles, control of cattle tuberculosis (TB) is hindered by persistent infection of wild badger (Meles meles) populations. A large-scale field trial—the Randomised Badger Culling Trial (RBCT)—previously showed that widespread badger culling produced modest reductions in cattle TB incidence during culling, which were offset by elevated TB risks for cattle on adjoining lands. Once culling was halted, beneficial effects inside culling areas increased, while detrimental effects on adjoining lands disappeared. However, a full assessment of the utility of badger culling requires information on the duration of culling effects.
Methodology/Principal Findings: We monitored cattle TB incidence in and …
Porcine Reproductive And Respiratory Syndrome Virus Nonstructural
Protein 1Β Modulates Host Innate Immune Response By
Antagonizing Irf3 Activation,
2010
University of Nebraska-Lincoln
Porcine Reproductive And Respiratory Syndrome Virus Nonstructural Protein 1Β Modulates Host Innate Immune Response By Antagonizing Irf3 Activation, Lalit Beura, Saumendra Sarkar, Byungjoon Kwon, Sakthivel Subramaniam, Clinton J. Jones, Asit K. Pattnaik, Fernando A. Osorio
School of Veterinary and Biomedical Sciences: Faculty Publications
Porcine reproductive and respiratory syndrome virus (PRRSV) infection of swine leads to a serious disease characterized by a delayed and defective adaptive immune response. It is hypothesized that a suboptimal innate immune response is responsible for the disease pathogenesis. In the study presented here we tested this hypothesis and identified several nonstructural proteins (NSPs) with innate immune evasion properties encoded by the PRRS viral genome. Four of the total ten PRRSV NSPs tested were found to have strong to moderate inhibitory effects on beta interferon (IFN-β) promoter activation. The strongest inhibitory effect was exhibited by NSP1 followed by, NSP2, NSP11, …
The Minor Envelope Glycoproteins Gp2a And Gp4 Of Porcine
Reproductive And Respiratory Syndrome Virus Interact
With The Receptor Cd163,
2010
University of Nebraska-Lincoln
The Minor Envelope Glycoproteins Gp2a And Gp4 Of Porcine Reproductive And Respiratory Syndrome Virus Interact With The Receptor Cd163, Phani B. Das, Phat X. Dinh, Israrul H. Ansari, Marcelo De Lima, Fernando A. Osorio, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
Porcine reproductive and respiratory syndrome virus (PRRSV) contains the major glycoprotein, GP5, as well as three other minor glycoproteins, namely, GP2a, GP3, and GP4, on the virion envelope, all of which are required for generation of infectious virions. To study their interactions with each other and with the cellular receptor for PRRSV, we have cloned each of the viral glycoproteins and CD163 receptor in expression vectors and examined their expression and interaction with each other in transfected cells by coimmunoprecipitation (co-IP) assay using monospecific antibodies. Our results show that a strong interaction exists between the GP4 and GP5 proteins, although …
An Investigation Of Humeral Stress Fractures In Racing Thoroughbreds Using A 3d Finite Element Model In Conjunction With A Bone Remodeling Algorithm,
2010
California Polytechnic State University, San Luis Obispo
An Investigation Of Humeral Stress Fractures In Racing Thoroughbreds Using A 3d Finite Element Model In Conjunction With A Bone Remodeling Algorithm, Ryan James Moore
Master's Theses
The humerus of a racing horse Thoroughbred is highly susceptible to stress fractures at a characteristic location as a result of cyclic loading. The propensity of a Thoroughbred to exhibit humeral fracture has made equines useful models in the epidemiology of stress fractures. In this study, a racing Thoroughbred humerus was simulated during training using a 3D finite element model in conjunction with a bone remodeling algorithm. Nine muscle forces and two contact forces were applied to the 3-dimensional finite element model, which contains four separate load cases representing fore-stance, mid-stance, aft-stance, and standing. Four different training programs were incorporated …
Dairy Animal Care: Quality Assurance,
2010
University of Nebraska - Lincoln
Dairy Animal Care: Quality Assurance
Nebraska Beef Quality Assurance Program
Dairy producers are encouraged to participate in the voluntary Dairy Animal Care and Quality Assurance (DACQA) Certification program. The purpose of this program is to enhance and demonstrate quality animal care practices, which assure food safety, quality and value as well as enhance consumer confidence in the milk and beef products that are harvested from cattle on America’s dairy farms.
Identification Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) First-Stage Larvae In The Feces Of Gray Wolves (Canis Lupus) By Molecular Methods,
2010
University of Saskatchewan
Identification Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) First-Stage Larvae In The Feces Of Gray Wolves (Canis Lupus) By Molecular Methods, Heather M. Bryan, Kathrin A. Sim, Chris T. Darimont, Paul C. Paquet, Brent Wagner, Violeta Muñoz-Fuentes, Judit E. Smits, Nell B. Chilton
Parasitology Collection
First-stage nematode larvae with a dorsal-spine (DSL) were detected in five of 1,565 fecal samples from gray wolves (Canis lupus) collected in British Columbia, Canada, between 2005 and 2008. Molecular techniques were used to identify the DSL because it was not possible to determine their species identity using morphologic characters. The DSL were identified as Parelaphostrongylus odocoilei based on the results of single-strand conformation polymorphism (SSCP) analyses and DNA sequencing of the ribosomal DNA first and second internal transcribed spacers. Finding DSL of P. odocoilei in the feces of gray wolves was unexpected because P. odocoilei adults are parasites of …
An Enzootic Vector-Borne Virus Is Amplified At Epizootic Levels By An Invasive Avian Host,
2010
University of Tulsa
An Enzootic Vector-Borne Virus Is Amplified At Epizootic Levels By An Invasive Avian Host, Valerie A. O'Brien, Amy T. Moore, Ginger R. Young, Nicolas Komar, William K. Reisen, Charles R. Brown
Zoonotics and Wildlife Disease: Publications
Determining the effect of an invasive species on enzootic pathogen dynamics is critical for understanding both human epidemics and wildlife epizootics. Theoretical models suggest that when a naive species enters an established host–parasite system, the new host may either reduce (‘dilute’) or increase (‘spillback’) pathogen transmission to native hosts. There are few empirical data to evaluate these possibilities, especially for animal pathogens. Buggy Creek virus (BCRV) is an arthropod-borne alphavirus that is enzootically transmitted by the swallow bug (Oeciacus vicarius) to colonially nesting cliff swallows (Petrochelidon pyrrhonota). In western Nebraska, introduced house sparrows (Passer domesticus …
Molecular Genealogy Tools For White-Tailed Deer With Chronic Wasting Disease,
2010
University of California - Davis
Molecular Genealogy Tools For White-Tailed Deer With Chronic Wasting Disease, Holly B. Ernest, Bruce R. Hoar, Jay A. Well, Katherine I. O'Rourke
Zoonotics and Wildlife Disease: Publications
Molecular genetic data provide powerful tools for genealogy reconstruction to reveal mechanisms underlying disease ecology. White-tailed deer (Odocoileus virginianus) congregate in matriarchal groups; kin-related close social spacing may be a factor in the spread of infectious diseases. Spread of chronic wasting disease (CWD), a prion disorder of deer and their cervid relatives, is presumed to be associated with direct contact between individuals and by exposure to shared food and water sources contaminated with prions shed by infected deer. Key aspects of disease ecology are yet unknown. DNA tools for pedigree reconstruction were developed to fill knowledge gaps in …
Experimental Oral Transmission Of Chronic Wasting Disease To Red Deer (Cervus Elaphus Elaphus): Early Detection And Late Stage Distribution Of Protease-Resistant Prion Protein,
2010
Canadian Food Inspection Agency
Experimental Oral Transmission Of Chronic Wasting Disease To Red Deer (Cervus Elaphus Elaphus): Early Detection And Late Stage Distribution Of Protease-Resistant Prion Protein, Aru Balachandran, Noel P. Harrington, James Algire, Andrei Soutyrine, Terry R. Spraker, Martin Jeffrey, Lorenzo González, Katherine I. O'Rourke
Zoonotics and Wildlife Disease: Publications
Chronic wasting disease (CWD), an important emerging prion disease of cervids, is readily transmitted by intracerebral or oral inoculation from deer-to-deer and elk-to-elk, suggesting the latter is a natural route of exposure. Studies of host range susceptibility to oral infection, particularly of those species found in habitats where CWD currently exists are imperative. This report describes the experimental transmission of CWD to red deer following oral inoculation with infectious CWD material of elk origin. At 18 to 20 months post-inoculation, mild to moderate neurological signs and weight loss were observed and animals were euthanized and tested using 3 conventional immunological …
Detection Of The Abnormal Isoform Of The Prion Protein Associated With Chronic Wasting Disease In The Optic Pathways Of The Brain And Retina Of Rocky Mountain Elk (Cervus Elaphus Nelsoni),
2010
Colorado State University - Fort Collins
Detection Of The Abnormal Isoform Of The Prion Protein Associated With Chronic Wasting Disease In The Optic Pathways Of The Brain And Retina Of Rocky Mountain Elk (Cervus Elaphus Nelsoni), T. R. Spraker, Katherine I. O'Rourke, T. Gidlewski, J. G. Powers, J. J. Greenlee, M. A. Wild
Zoonotics and Wildlife Disease: Publications
Eyes and nuclei of the visual pathways in the brain were examined in 30 Rocky Mountain elk (Cervus elaphus nelsoni) representing 3 genotypes of the prion protein gene PRNP (codon 132: MM, ML, or LL). Tissues were examined for the presence of the abnormal isoform of the prion protein associated with chronic wasting disease (PrPCWD). Nuclei and axonal tracts from a single section of brain stem at the level of the dorsal motor nucleus of the vagus nerve were scored for intensity and distribution of PrPCWD immunoreactivity and degree of spongiform degeneration. This obex scoring …
Prion Genotypes Of Scrapie-Infected Canadian Sheep 1998–2008,
2010
Canadian Food Inspection Agency
Prion Genotypes Of Scrapie-Infected Canadian Sheep 1998–2008, Noel P. Harrington, Katherine I. O'Rourke, Yuquin Feng, Jasmine Rendulich, Cathleen Difruscio, Aru Balachandran
Zoonotics and Wildlife Disease: Publications
This report describes the genetics of the prion protein gene (PRNP) at codons 136, 154, and 171 for sheep diagnosed with naturally acquired classical scrapie in Canada between 1998 and 2008. Genotyping analysis was performed on 249 sheep with confirmed classical scrapie infection representing 98 flocks from 6 provinces. A further case-control analysis of 3 of these flocks compared the genotypes between infected sheep (n = 72) and those of their healthy flockmates (n = 1990). The incidence of classical scrapie in the Canadian sheep population was highly associated with the ARQ haplotype (91.8%) and the …
Increased Risk Of Chronic Wasting Disease In Rocky Mountain Elk Associated With Decreased Magnesium And Increased Manganese In Brain Tissue,
2010
U.S. Department of Agriculture
Increased Risk Of Chronic Wasting Disease In Rocky Mountain Elk Associated With Decreased Magnesium And Increased Manganese In Brain Tissue, Stephen N. White, Katherine I. O'Rourke, Thomas Gidlewski, Kurt C. Vercauteren, Michelle R. Mousel, Gregory E. Phillips, Terry R. Spraker
Zoonotics and Wildlife Disease: Publications
Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) of Rocky Mountain elk in North America. Recent studies suggest that tissue and blood mineral levels may be valuable in assessing TSE infection in sheep and cattle. The objectives of this study were to examine baseline levels of copper, manganese, magnesium, zinc, selenium, and molybdenum in the brains of Rocky Mountain elk with differing prion genotypes and to assess the association of mineral levels with CWD infection. Elk with leucine at prion position 132 had significantly lower magnesium levels than elk with 2 copies of methionine. Chronic wasting disease-positive elk …
School Of Veterinary Medicine & Biomedical Sciences: 2010 Annual Report,
2010
University of Nebraska - Lincoln
School Of Veterinary Medicine & Biomedical Sciences: 2010 Annual Report
School of Veterinary and Biomedical Sciences: Information and History
On September 4, 2009, The Department of Veterinary Medicine and Biomedical Sciences was renamed the School of Veterinary Medicine and Biomedical Sciences. The School is informally organized into six functional areas: • Undergraduate Teaching • Research and Graduate Studies • Extension • Veterinary Diagnostic Center (VDC) • Great Plains Veterinary Educational Center (GPVEC) • Professional Program in Veterinary Medicine (PPVM) While teaching and research activities bridge the entire faculty, subsets of the faculty are primarily involved with one area, i.e., the undergraduate program, the research and graduate program, the veterinary medical students clinical teaching at the GPVEC, the pre-clinical teaching …
Epizootiologic Survey Of Mycobacterium Bovis In Wildlife And Farm Environments In Northern Michigan,
2010
USDA-APHIS-Wildlife Services
Epizootiologic Survey Of Mycobacterium Bovis In Wildlife And Farm Environments In Northern Michigan, Gary W. Witmer, Amanda E. Fine, James Gionfriddo, Michael Pipas, Kirk Shively, Kim Piccolo, Patrick Burke
Michigan Bovine Tuberculosis Bibliography and Database
Bovine tuberculosis (bovine TB), caused by Mycobacterium bovis, has reemerged in northern Michigan, USA, with detections in white-tailed deer (Odocoileus virginianus) in 1994 and in cattle in 1998. Since then, significant efforts have been directed toward reducing deer densities in the area in the hopes of reducing the bovine TB prevalence rate in deer and eliminating spillover of the disease into cattle. Despite the success of the efforts to reduce deer densities, additional cattle herds have become infected. Other mammals can be infected with M. bovis, and some carnivores and omnivores had been found to be infected …
