Veterinary Medicine Commons^™

Intrauterine Insemination With Cat Semen Frozen With Various Extenders, Alper Baran, Çağatay Tek, Kamber Demi̇r, Ahmet Sabuncu, Özen Banu Özdaş

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the post-thaw motility and morphological defect rates of cat semen frozen with various extenders, and pregnancy rates after intrauterine insemination (IUI). Cats, 2 males and 14 queens, 2 to 3 years of age, and under the same managemental conditions were used. Semen was collected by means of an electro-ejaculator under general anesthesia. Based on spermatological traits, semen samples presumed suitable for freezing were extended with either tris-fructose-citric acid (EY-TFC) containing 20% egg yolk, or skimmed milk-glucose-taurine (EY-SMGT) containing 10% egg yolk extender, and frozen in 0.25 mL straws. For the extenders, post-thaw …

A Comparison Of Different Kinds Of Malassezia Species In Healthy Dogs And Dogs With Otitis Externa And Skin Lesions, Samaneh Eidi, Ali Reza Khosravi, Shahram Jamshidi

Turkish Journal of Veterinary & Animal Sciences

Malassezia yeasts are commensal organisms on the skin of warm-blooded vertebrates. The lipid-dependent Malassezia species have recently been cultured from veterinary specimens. The present study investigated and compared different Malassezia species in the skin and external ear canal of healthy and diseased dogs. The sampling in the study was carried out on 152 animals, comprising 90 samples from the diseased group and 62 samples from the healthy group. All of the samples were determined by cytological examination and fungal culture. The isolated yeasts were identified by their morphological features as well as their physiological characteristics. The culture results were positive …

Malignant Giant-Cell Tumor Of Bone With Lymph Node Involvement In A Cat, Ahmet Akkoç, Gürsel Sönmez, Kemal Yanik, Ayli̇n Alasonyalilar Demi̇rer, Ni̇hal Y. Gül, M. Carmen Ferreras-Estrada

Turkish Journal of Veterinary & Animal Sciences

The present study describes giant-cell tumor of bone (GCToB) with lymph node involvement in a 5-year-old crossbred cat. The animal was referred to the surgery clinic with progressive subcutaneous swelling in the left proximal femoral region, severe lameness, constipation, and dysuria. A moderately firm, subcutaneous, palpable mass, 9 cm in diameter, was observed, and biopsy samples were taken. Histopathologically, the mass was constituted by ovoid-shaped mononuclear cells intermixed with many multinucleated giant cells (MGC). Immunohistochemically, the giant cells were positively stained with antivimentin, and the same cells were negative for antidesmin and anti-S100 staining. Tartrate-resistant acid phosphatase (TRAP) activity in …

The Occipital Area In Medieval Dogs And The Role Of Occipital Dysplasia In Dog Breeding, Maciej Janeczek, Aleksander Chrószcz

Turkish Journal of Veterinary & Animal Sciences

An investigation was carried out on 42 dog skulls from the early medieval period. The skulls were excavated in Wroclaw, Poland, and in Novgorod and Moscow, Russia. Craniometric measurements were taken. On the basis of the basion-ethmoid measurement, the shoulder height was estimated. The foramen magnum height and width were measured and the foramen magnum index was calculated. The foramen magnum was typical in shape, and any occipital dysplasia signs observed were in the skulls. The results of the investigation, in comparison to other investigations of modern breeds and archaeozoological material, suggest that occipital dysplasia is not a pathology but …

Serological Study Of Leptospirosis Among Dairy Cattle In Bosnia And Herzegovina, Maid Rifatbegovic, Zinka Maksimovic

Turkish Journal of Veterinary & Animal Sciences

A total of 75,206 blood serum samples from dairy cattle and quarantined heifers was collected between 2001 and 2007 and analyzed for bovine leptospirosis by the microscopic agglutination test (MAT). Serovar Hardjo was used for testing of all sera, with the addition of 2 or 3 randomly chosen other serovars (Grippotyphosa, Icterohaemorrhagiae, Pomona, Bataviae, Canicola, and/or Saxcoebing). From the total number of sera tested, 1197 (1.59%) were serologically positive, with a decreasing tendency over the years of research. The most prevalent serovar was Pomona (1.32%), followed by Hardjo (0.52%) and Grippotyphosa (0.37%). The differences among the studied regions in terms …

Maedi-Visna Virus Infection In A Merino Lamb With Nervous Signs, Ahmet Akkoç, Meri̇ç Kocatürk, Ayli̇n Alasonyalilar Demi̇rer, Sezgi̇n Şentürk, Giacomo Renzoni, Silvia Preziuso

Turkish Journal of Veterinary & Animal Sciences

A Maedi-Visna virus (MVV) infection was diagnosed in a 10-month-old lamb from a Turkish flock. The animal suddenly showed nervous signs and died within 2 days. At necropsy, different pulmonary and nervous lesions were observed. A Coenurus cerebralis cyst was also present in the right-brain hemisphere. Severe nonpurulent meningoencephalitis and lymphoproliferative pneumonia were detected by histopathology. MVV proviral DNA was demonstrated by polymerase chain reaction (PCR) in formalin-fixed and paraffin-embedded samples collected from the brain and the lungs. This unusual report in a young animal confirms that not only adult sheep but also lambs can develop MVV disease. The hypothesis …

Molecular And Genomic Approaches To Understanding Host-Virus Interactions In Shaping The Outcome Of Equine Arteritis Virus Infection, Yun Young Go

University of Kentucky Doctoral Dissertations

Equine arteritis virus (EAV) is the causal agent of equine viral arteritis, a disease of equids. During natural outbreaks of the disease, EAV can cause abortion in pregnant mares and persistent infection in stallions. Understanding how host cellular proteins interact with viral RNA and viral proteins, as well as their role in viral infection, will enable better characterization of the pathogenesis of EAV and establishment of persistent infection in stallions. Accordingly, we hypothesized that both viral factors and host genetically related factors could influence the outcome of EAV infection in horses. To test this hypothesis, we first combined contemporary molecular …

Pituophis Catenifer Deserticola (Great Basin Gophersnake) Predation, Steven J. Wamback, Richard M. Engeman

United States Department of Agriculture Wildlife Services: Staff Publications

A variety of taxa have been observed to· prey upon Pituophis catenifer, including mammals, birds-of prey, and other snakes. Here, we describe an unusual two-day observation of predation on a mature Great Basin gophersnake by a brown trout (Salmo trutta).

The observation took place in Jones Canyon, Utah, USA, on the grounds of the Jones Hole National Fish Hatchery (JHNFH). Jones Creek' emanates from springs at the head of Jones Canyon on hatchery grounds, and flows north to south 6.4 km to the Green River, approximately paralleling the Utah-Colorado border. The stream runs for about 0.3 km …

Rodents And Other Vertebrate Invaders In The United States, Michael W. Fall, Michael L. Avery, Tyler A. Campbell, Peter J. Egan, Richard M. Engeman, David Pimentel, William C. Pitt, Stephanie A. Shwiff, Gary W. Witmer

United States Department of Agriculture Wildlife Services: Staff Publications

Contents

Introduction

Assessing impacts of rodents and other vertebrate invaders

Accounts of some important vertebrate invaders
Norway rat (Rattus norvegicus)
Roof rat (Rattus rattus)
Polynesian rat (Rattus exulans)
House mouse (Mus Musculus)
Nutria (Myocastor coypus)
Gambian giant pouched rat (Cricetomys gambianus)
Feral swine (Sus scofa)
Small Indian Mongoose (Herpestes javanicus)
Rock pigeon (Columba livia)
House sparrow (Passer domesticus)
European starling (Sturnus vulgaris)
Monk parakeet (Myiopsitta monachus)
Brown tree snake (Boiga irregularis)
Burmese python (Python …

Cystic Ovaries In Dairy Cattle, Lacey Morgan Rosenberg

Dairy Science

The objective of this literature review was to determine the potential causes and factors affecting the incidence of ovarian cysts in dairy cattle as well as to examine functionality of various preventative methods and treatments of cysts. The end product was to be a document outlining all the information available on the three different types of cysts that can develop on the bovine ovary, follicular cysts, luteal cysts, and cystic corpora lutea. The literature that was reviewed, most in the forms of research papers, articles, and reviews, came primarily from scientific journals and published books. The literature reviewed was only …

Characterization Of The Sero-Reactivity Of Proteins Map1152 And Map1156 From Mycobacterium Avium Subspecies Paratuberculosis, Avery L. Paulson

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease (JD) in ruminants. Development of genetic tools and completion of the MAP genome sequencing project expanded opportunities for antigen discovery. In this thesis, I review the current trends in diagnosis and disease control of JD and present the results of the studies on the seroreactivity of two proteins encoded for by the MAP1152-MAP1156 gene cluster. MAP1152 encodes for a PPE protein and MAP1156 encodes a diacylglycerol acyltransferase involved in triglyceride metabolism and classified in the uncharacterized protein family UPF0089. Maltose-binding protein (MBP) tagged recombinant MAP proteins were purified from Escherichia coli. …

How I Became A Scientist: Activity Book For 3rd Graders, Jessica Schneider, Kauline Davis, Thad Blossom

College of Veterinary Medicine eBooks

This activity book highlights how eight people became scientists from all over the world who work with animals. These scientists work hard to make sure that people and their animals stay healthy. Activities in the book cover topics related to each scientist's area of study.

Published in full color, the book features fun activities relevant to the different scientific specialties represented for children to complete. The publication of the book has been supported by the National Institutes of Health, the National Center for Research Resources, and a Scientific Education Partnership Award.

Staphylococcus Aureus Virulence Factors Synthesis Is Controlled By Central Metabolism, Yefei Zhu

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Staphylococcus aureus is a versatile pathogen that can survive in diverse host environments. This versatility depends on its ability to sense nutrients and respond by modulating gene expression, including the synthesis of virulence determinants. In addition to its ability to synthesize virulence factors, the capacity of S. aureus to form biofilms is an important mediator of virulence in certain infections. Biofilms are a complex aggregation of bacteria commonly encapsulated by an adhesive exopolysaccharide matrix (polysaccharide intercellular adhesin; PIA). To study S. aureus biofilm formation, we assessed the metabolic requirements of S. aureus growing in a biofilm and …

Swine High Fever Syndrome: What Prrsv Gotta Do With It?, Roongroje Thanawongnuwech

The Thai Journal of Veterinary Medicine

No abstract provided.

Effects Of Dietary Melamine Or Urea-Formaldehyde Or Their Mixtures On Performance, Carcass Quality, Melamine Residues And Microscopic Changes In Broiler Tissues, Srisuda Sirilaophaisan, Jowaman Khajarern, Bundit Tengjarernkul

The Thai Journal of Veterinary Medicine

A study was conducted to determine the effects of dietary graded levels of melamine, urea-formaldehyde (UF) and their mixture on growth performance, carcass quality, melamine residues and microscopic changes in broiler tissues from d-1 to d-42 followed by a 7-d feeding of withdrawal diets. One thousand and forty 1-d-old equal mixed-sex Arber Acres broiler were assigned to 13 dietary treatments with 4 replicate pens of 20 chicks assigned to each treatment. The diets of melamine, UF and their mixture contained four graded levels (0.25, 0.50, 0.75 and 1.00%) but no melamine or UF was added to the control diet. There …

Chronic Toxicity Study Of Kaempferia Parviflora Wall Ex. Extract, Songpol Chivapat, Pranee Chavalittumrong, Aimmanas Attawish, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

K. parviflora is a medicinal plant possessing high potential for development of various health products. The objective of this chronic toxicity study was to investigate the safety of ethanolic extract of chronic Kaempferia parviflora Wall ex Bak in Wistar rats. The animals were randomly divided into five groups, twenty four rats each (12 males and 12 females). Three treatment groups were orally administered with K. parviflora extract at doses of 5, 50 and 500 mg/kg/day for six months respectively, which were equivalent to 1, 10 and 100 times of human use, while two control groups were orally given with distilled …

Molecular Classification Of Infectious Laryngotracheitis Virus From Chick Embryo Origin Vaccine, Tissue Culture Origin Vaccine And Field Isolates, Wisanu Wanasawaeng, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The molecular classification of the infectious laryngotracheitis virus from chicken embryo origin (CEO) vaccine, tissue culture origin (TCO) vaccine and field isolates by the sequence of ICP4 genes from CEO and TCO vaccines in the GenBank was performed for the different sequence analysis. The sequences of TCO vaccine virus (accession no. EU104908) were used as a prototype for a primer design to cover the region of 450 basepairs (bp) by Primer3 software. The simulation sequences of CEO vaccine virus (accession no. EU104900) and TCO vaccine virus (accession no. EU104908) from PCR reaction were selected for restriction enzyme. Hga I could …

Tetranucleotide Microsatellite Markers For Molecular Testing In Thai Domestic Elephants (Elephas Maximus Indicus), Duangsmorn Suwattana, Jutarat Jirasupphachok, Sumolya Kanchanapangka, Weerapong Koykul

The Thai Journal of Veterinary Medicine

Eleven microsatellite loci were evaluated for genetic profiles of Thai domestic elephants (Elephas maximus indicus) and their suitability as genetic markers for molecular testing. A total of 66 animals were tested. 10 out of 11 loci could be amplified and they demonstrated polymorphism with allelic numbers per locus ranging from 7 (LaT06) to 39 (LaT18). Values of expected heterozygosity (He) and Polymorphic Information Content (PIC) were between 0.6449 (LaT17) – 0.9593 (LaT05) and 0.5934 (LaT17) – 0.9578 (LaT05), respectively. Analysis of the ten microsatellite markers revealed Combined Exclusion Probability (CEP) of 99.99998783% or 1.2167 x 10-7 and 99.91% confident for …

Comparative Study Between Using Acetone And Absolute Alcohol For Dehydration In Plastination Procedure, Kongkiat Srisuwatanasagul, Sayamon Srisuwatanasagul, Adisorn Adirekthaworn, Damri Darawiroj

The Thai Journal of Veterinary Medicine

In order to develop the most practical plastination specimens used for anatomical study, the aim of the present study was to compare between using acetone and absolute alcohol for dehydration in plastination procedure. Ten pig heart specimens were used in the present study and plastination procedure was processed by using acetone (group 1) or absolute alcohol (group 2) for dehydration. The results showed that dehydration by acetone resulted in more natural color with no shrinkage of the heart specimens while dehydration by alcohol caused darker color and more shrinkage. However, the advantage of using alcohol was that it gave the …

Immune Cell Population In Oviducts In Relation To Ovarian And Progesterone Status Of Anoestrous And Repeat Breeding Gilts, Paisan Tienthai, Sakaoporn Prachantasena, Supassama Chaiyawong, Tharnath Nanthirudjanar, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The aim of the present study is to determine the distribution of immune cells within the endosalpinx related to the ovarian function in gilts culled due to repeat breeding and anoestrus. A total of 30 reproductive organs from culling crossbred Landrace x Yorkshire gilts were collected from commercial swine herds in Thailand and categorized into 3 groups, i.e. anoestrus, repeat breeding and non-involved in reproductive disorder gilts (control group). The ovaries and oviducts were separated from the reproductive tracts and macroscopically investigated. The oviducts were divided into the utero-tubal junction (UTJ), the isthmus, the ampulla and the infundibulum. The gilts …