Arginine Metabolism In The Edwardsiella Ictaluri- Channel Catfish Macrophage Dynamic,
2011
Louisiana State University and Agricultural and Mechanical College
Arginine Metabolism In The Edwardsiella Ictaluri- Channel Catfish Macrophage Dynamic, Wes Arend Baumgartner
LSU Doctoral Dissertations
Edwardsiella ictaluri encodes a urease operon and an arginine decarboxylase (AdiA) that are required for virulence in head kidney derived macrophages (HKDM). The urease produces ammonia in amounts sufficient to alter environmental pH from acid to neutral. A hypothetical model was proposed, involving arginine metabolism in E. ictaluri infected HKDM, focusing on bacterial urease, AdiA, a second arginine decarboxylase (SpeA), and agmatinase (SpeB). Using fluorescence based ratiometric pH determination of E. ictaluri in live HKDM, it was shown that E. ictaluri modulates HKDM phagosome pH to above six. Urease and AdiA mutants failed to up-regulate vacuole pH, while vacuole pH …
Mapping And Modeling Of Neglected Tropical Diseases In Brazil And Bolivia,
2011
Louisiana State University and Agricultural and Mechanical College
Mapping And Modeling Of Neglected Tropical Diseases In Brazil And Bolivia, Paula Mischler
LSU Doctoral Dissertations
Accurately defining disease distributions and calculating disease risk is an important step in the control and prevention of diseases. This study used geographical information systems and remote sensing technologies within the MaxEnt ecological niche modeling program to create predictive risk maps for leprosy and Schistosomiasis in Brazil and Chagas disease in both Brazil and Bolivia. New disease cases were compiled for leprosy, Schistosomiasis, and Chagas disease from the Brazilian ministry of Health for 2001 to 2009 and the data was stratified to a 10,000 population for each municipality. Bolivian Chagas prevalence rates were calculated from 2007 to 2009 survey data. …
The Tick Response To Rickettsial Dissemination During Typical And Atypical Rickettsial Infection,
2011
Louisiana State University and Agricultural and Mechanical College
The Tick Response To Rickettsial Dissemination During Typical And Atypical Rickettsial Infection, Piyanate Sunyakumthorn
LSU Doctoral Dissertations
Ticks are the only disease vectors for spotted fever group (SFG) Rickettsia which are obligate intracellular bacteria belonging to the genus Rickettsia. In nature, ticks maintain the infection of SFG Rickettsia via vertical and horizontal transmission. However, the prevalence of rickettsial transmission is limited to certain species of ticks, and this limitation is known as a specific tick/Rickettsia relationship. Due to the continuous increase of tick-borne rickettsial disease cases in the United States, which contrasts with very low prevalence of Rickettsia in tick vectors, the study of vector competence of tick to Rickettsia is needed in order to understand the …
Arbovirus Phenotype Alters Transmission Potential,
2011
Louisiana State University and Agricultural and Mechanical College
Arbovirus Phenotype Alters Transmission Potential, Rebecca C. Christofferson
LSU Doctoral Dissertations
Extrinsic and environmental factors are known to affect the transmission of arthropod-borne viruses (arboviruses), including variations in the arthropod vector populations. Differences among these factors have been associated with differential transmission and are sometimes used to control the spread of an arbovirus through a vertebrate population in an effort to prevent or disrupt an outbreak. However, diversity in intrinsic viral populations, such as genetic and phenotypic variability, is not often accounted for when considering alterations in transmission. Presented in this dissertation are four experimental studies that explore the contribution of viral intrinsic factors, especially phenotypic variability, to the transmission potential …
Influence Of Pgf_{2\Alpha} On Semen Quality And Libido In Holstein Bulls,
2011
TÜBİTAK
Influence Of Pgf_{2\Alpha} On Semen Quality And Libido In Holstein Bulls, Reza Masoumi, Armin Towhidi, Ardsher N. Javaremi, Habib Nabizadeh, Mehdi Zhandi
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the effects of Cloprostenol (PGF_{2\alpha} analogue) on semen quality and libido in Holstein bulls. Ten low libido Iranian Holstein bulls were randomly allocated to 2 groups and received Cloprostenol (n = 5) or saline (n = 5) 30 min prior to the semen collection 2 days per week for 2 months. Reaction time was significantly decreased in the treatment group. Duration of ejaculation was significantly increased in the treatment group. Semen volume and sperm concentration were higher in treated bulls when compared with controls. The percentage of morphologically normal sperm cells, percentage …
Stage-Specific Developmental Gene Expression Of Goat Preimplantation Embryos Produced In Vitro,
2011
TÜBİTAK
Stage-Specific Developmental Gene Expression Of Goat Preimplantation Embryos Produced In Vitro, Y. J. Li, H. Ao, G. J. Sun, L. Zhang
Turkish Journal of Veterinary & Animal Sciences
Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had …
Association Analysis Of Aquaporin 7 (Aqp7) Gene Variants With Semen Quality And Fertility In Bulls,
2011
TÜBİTAK
Association Analysis Of Aquaporin 7 (Aqp7) Gene Variants With Semen Quality And Fertility In Bulls, Tenghe Ma, Jifeng Liu, Ruifeng Zhao, Hao Jiang, Lisheng Dai, Yumin Zhao, Zhihui Zhao, Jiabao Zhang
Turkish Journal of Veterinary & Animal Sciences
Aquaporin 7 (AQP7) gene as a candidate Antifreeze gene was investigated and associated with fresh and frozen semen quality traits in 45 Simmental and Charolais bulls. PCR-SSCP, PCR-RFLP, and sequencing method were employed to detect the single nucleotide polymorphisms (SNPs) of the AQP7 gene. Two mutations, A264G and G371C, were identified. The allele frequencies were A (0.97, 0.96) to B (0.03, 0.04) and C (0.97, 0.91) to D (0.03, 0.09) in Simmental and Charolais bulls, respectively. A264G substitution was significantly associated with acrosome integrity ratio (P < 0.01) and motility (P < 0.05) in frozen semen. Meanwhile, G371C substitution was significantly associated with acrosome integrity ratio (P < 0.05), percentages of viable sperm (P < 0.05), and motility (P < 0.01) in frozen semen. The results prove that AQP7 gene SNPs are useful for marker-assisted selection in early seed selection in the bull industry.
Alteration Of Gene Expression In Microvascular Endothelial Cells After Exposure To Foot-And-Mouth Disease Virus 146s Antigen,
2011
TÜBİTAK
Alteration Of Gene Expression In Microvascular Endothelial Cells After Exposure To Foot-And-Mouth Disease Virus 146s Antigen, Tao Zhang, Shuang Zhang, Li-Fang Tian, Ge Hu, Zhan-Wei Suo, Wu Chen, Hui-Qin Duan, Xiang Mu, Ke-Dao Teng
Turkish Journal of Veterinary & Animal Sciences
To investigate the global response of microvascular endothelial cells (MVECs) to foot-and-mouth (FMDV)disease vaccine (FMDV) antigen and to explore the role of MVECs in FMD vaccine immunity, the gene expression alterations of rat myocardium microvascular endothelial cells (RMMVECs) after stimulation with FMD virus (FMDV) 146S antigen were assayed using cDNA microarrays. We observed that 18 genes and 67 genes in RMMVECs were respectively altered by FMDV 146S antigen at 6 h and 24 h, respectively. The former were all up-regulated genes, and the latter included 60 up-regulated genes and 7 down-regulated genes. Among the differentially expressed genes, many were associated …
Duration Of Immunity Following The Administration Of Oil-Based Avian Influenza H_5n_1 Vaccine In Layers,
2011
TÜBİTAK
Duration Of Immunity Following The Administration Of Oil-Based Avian Influenza H_5n_1 Vaccine In Layers, Maryam Nisar, Anwar-Ul Haq, Asif Rashid, Muhammad Iqbal
Turkish Journal of Veterinary & Animal Sciences
Avian influenza (AI) occurs worldwide and causes tremendous economic losses. The disease is characterised by respiratory signs, depression, and reduced food and water intake. In the present study, an oil-based vaccine created by using Montanide ISA 70 MVG, was prepared and the duration of immunity checked at different time intervals. For this purpose, the cumulative mean titre (CMT) was calculated after employing haemagglutination inhibition test in 50 pullets at day zero before vaccination and then inoculated 0.3 mL of emulsified vaccine subcutaneously in 40 birds while 10 birds were kept non-vaccinated as a control group throughout the experiment. On day …
Effects Of Dietary Fish Oil Replacement By Cottonseed Oil On Growth Performance And Fatty Acid Composition Of Rainbow Trout (Oncorhynchus Mykiss), Müjde Güler, Mustafa Yildiz
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to determine the effects of replacing fish oil (FO) with cottonseed oil (CSO), as an alternative plant lipid source in diets for rainbow trout (89.3 ± 1.1 g mean individual weight), on the growth and fatty acid composition of fish. As a control diet, 5 experimental diets were formulated with pure FO and with partial or complete (25%, 50%, 75%, or 100% CSO, respectively) replacement of FO with CSO. The fish were fed 2% of their body weight per day for 60 days at an average water temperature of 10.2 °C. At the …
Monitoring Of Somatic Cell Count Variations During Lactation In Primiparous And Multiparous Turkish Saanen Goats (Capra Hircus), Abdülkadi̇r Orman, Ayteki̇n Günay, Faruk Balci, Mehmet Koyuncu
Turkish Journal of Veterinary & Animal Sciences
In this study, a total of 28 Saanen does were used to evaluate the relationship between somatic cell count (SCC) and milk yield. The study included 14 first-lactation (primiparous) does and 14 does in second lactation or greater (multiparous). Individual milk samples from both morning and evening milkings were taken every 15 days throughout the lactation period of 7 months. While somatic cell counts were higher during the evening milking (840.40 versus 532.30 cells mL-1; 1050.40 versus 759.70 cells mL-1), milk yields were lower during the evening milking (115.40 versus 193.80 kg; 177.40 versus 280.00 kg) for both primiparous and …
Population Ecology Of A Phthirapteran Occurring On The Common Baya (Ploceus Philippinus) (Ploceidae: Passeriformes: Aves),
2011
TÜBİTAK
Population Ecology Of A Phthirapteran Occurring On The Common Baya (Ploceus Philippinus) (Ploceidae: Passeriformes: Aves), Gaurav Arya, Nayanci Bansal, Aftab Ahmad, Nidhi Gupta, Arun Kumar Saxena
Turkish Journal of Veterinary & Animal Sciences
One hundred common Bayas (Ploceus philippinus) were examined for their ectoparasites, during April 2006 to December 2008 in the Rampur district of India (28°49'12"N 79°1'11"E). Seventy-four animals were found infested (mean intensity 13.97, sample mean abundance 10.3, range of infestation 1-45) with an ischnoceran louse, Brueelia plocea. The frequency distribution pattern of the louse on the aforesaid bird was aggregated but did not conform to the negative binomial model. The sex ratio was female biased while the nymphal population was dominant over adults.
Comparison Of Placentome Diameters In Single And Twin-Pregnant Sheep By Ultrasonographic Method,
2011
TÜBİTAK
Comparison Of Placentome Diameters In Single And Twin-Pregnant Sheep By Ultrasonographic Method, Güven Kaşikçi, Özge Yilmaz, Mehmet Can Gündüz, İsmai̇l Kirşan
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the correlation between fetal number and placentome diameters in pregnant sheep. In the present study 25 Kıvırcık mixed breed pregnant ewes were used. Pregnancy of the ewes was diagnosed by transrectal ultrasonography by the 25th day after insemination. A transabdominal probe (6.5 MHz) was used on the pregnant ewes at the 30th day. At this time the placentomes appeared as hypoechogenic areas with a "C"-like shape. Throughout the pregnancies 5 placentomes were measured from each ewe every week. The results showed no significant difference between placentome diameter size in single and twin-pregnant …
Effects Of Halving Intravaginal Sponges And Ecg Dose On Estrus Response And Fertility In Tahirova Ewes During The Breeding Season, Güven Kaşikçi, Ümüt Ci̇ri̇t, Mehmet Can Gündüz, Süleyman Bacinoğlu, Ahmet Sabuncu
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to compare the effect of halving the intravaginal sponges impregnated with 20 mg of flugestone acetate (FGA; 20 or 10 mg) and a dose of equine chorionic gonadotropin (eCG; 600 or 300 IU) on fertility in Tahirova ewes. A total of 200 Tahirova ewes were randomly assigned to 1 of 2 groups during the breeding season. Half of the ewes (n = 100) were treated with whole intravaginal sponges (WS, 20 mg of FGA) and the other half (n = 100) with halved sponges (HS, 20 mg of FGA) for 14 days. The ewes …
Comparative Ultrasonographic Study Of The Prostate Complex And Bulbourethral Glands Of The Domestic Rabbit (Oryctolagus Cuniculus), Rosen Dimitrov, Kamelia Stamatova
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to provide a comparative description of the rabbit prostate complex and bulbourethral glands by means of ultrasonography, in order to obtain results that may be useful for the diagnosis of reproductive pathology in this species. The prostate and bulbourethral glands were studied sonographically in 12 sexually mature, clinically healthy male New Zealand rabbits that were 12 months old and weighed 2.8 to 3.2 kg. The prostate complex, composed of proprostate, prostate, and paraprostate parts, was observed using prepubic ultrasonography in sagittal and transversal planes and presented a hyperechoic stroma. The bulbourethral glands were …
Milk Composition And Yield Changes With Advancing Pregnancy In Dairy Buffaloes (Bubalus Bubalis),
2011
TÜBİTAK
Milk Composition And Yield Changes With Advancing Pregnancy In Dairy Buffaloes (Bubalus Bubalis), Sarzamin Khan, Muhammad Subhan Qureshi, Ifthiar Ahmed, Syed Mirajullah Shah
Turkish Journal of Veterinary & Animal Sciences
The changes in the composition of milk with advancing pregnancy were investigated in Nili-Ravi dairy buffaloes. Forty lactating buffaloes were used, within 2-3 months postpartum. The animals were grouped as high, moderate, and low yielders; synchronized for estrus; and inseminated artificially. Milk yield was recorded daily and sampled fortnightly for analysis. The data were analyzed using the univariate weighted mean and general linear model. Decline in milk yield became significant after the 8th week post-conception. The high yielders had the lowest fat contents. Milk fat increased significantly during succeeding weeks post-conception. The highest fat level (8.5%) was observed in week …
In Vitro Maturation And Fertilization Of Buffalo Oocytes: The Effect Of Recovery And Maturation Methods,
2011
TÜBİTAK
In Vitro Maturation And Fertilization Of Buffalo Oocytes: The Effect Of Recovery And Maturation Methods, Abid Mehmood, Muhammad Anwar, Sayed Murtaza Hassan Andrabi, Muhammad Afzal, Syed Muhammad Saqlan Naqvi
Turkish Journal of Veterinary & Animal Sciences
Conditions for in vitro maturation (IVM) of primary oocytes without conventionally used fetal calf serum and hormones were optimized in order to reduce the cost of laboratory produced buffalo embryos. Comparisons were made between oocyte recovery methods (aspiration vs. slicing) and IVM in medium 199 (static culture method vs. flux culture method) supplemented with 4-5 × 106 granulosa cells mL-1 that contained either estrus buffalo serum (EBS) or fetal calf serum (FCS). Recovery methods were compared according to yield, i.e. cumulus oocyte complexes per ovary (COCs/ovary), the expansion rate (% of COCs that expanded), and nuclear maturation rate (% of …
Computational Comparison Of Β-Mannosidases Of Animals, Humans, Microbes, And Plants,
2011
TÜBİTAK
Computational Comparison Of Β-Mannosidases Of Animals, Humans, Microbes, And Plants, Zahoor Qadir Samra, Muhammad Amin Athar
Turkish Journal of Veterinary & Animal Sciences
The \beta-mannosidase (MANB) enzyme is involved in removing mannose residue from the nonreducing end, and its impaired activity leads to \beta-mannosidosis. MANB amino acid sequences of humans, other mammals, plants, fungi, and bacteria were compared to determine their similarities, differences, and predicted 3D structures. Our cloned MANB DNA sequence showed a 99% similarity to a previously reported human MANB DNA sequence but 16 nucleotide differences were observed, showing the polymorphic nature of the enzyme. The 9 changed codons coded the different amino acids Ile, Lys, Ile, Thr, Arg, Leu, Leu, Gly, and Asp, while 7 changed codons coded the same …
Repeat Breeder Syndrome In Dairy Cows: Influence Of Breed And Age On Its Prevalence And The Success Of A Hormone Therapy*,
2011
TÜBİTAK
Repeat Breeder Syndrome In Dairy Cows: Influence Of Breed And Age On Its Prevalence And The Success Of A Hormone Therapy*, Robert Zobel, Suzana Tkalcic, Vlatka Buic, Ivana Pipal, Darko Geres, Marko Samardzija
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to establish the prevalence of repeat breeder syndrome (RBS) in different breeds and reproductive ages of cattle in central Croatia and to assess the effectiveness of hormone therapy as a treatment option. A total of 1088 dairy cows were observed, 218 heifers and 870 multiparous cows. After the third unsuccessful artificial insemination (n = 164) and a thorough clinical and gynecological examination, the cows were tested for sexually transmitted diseases. An RBS diagnosis was based on failure to conceive, negative laboratory tests for sexually transmitted diseases, and the absence of other reproductive disorders. The …
Growth Characteristics In Lambs Of Estrus Synchronized Ewes In Grower Conditions,
2011
TÜBİTAK
Growth Characteristics In Lambs Of Estrus Synchronized Ewes In Grower Conditions, Murat Yilmaz, Tufan Altin
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the growth characteristics of lambs of estrus-synchronized ewes in an extensive production system during 2 different periods. The animal material of this study was composed of 325 head Chios × Kıvırcık cross breeds. These lambs were provided by mating 124 Kıvırcık sheep on 2 farms with 8 Chios rams provided by the Adnan Menderes University, Faculty of Agriculture, Sheep Research Unit. The estrus cycles of the ewes were synchronized with intra-vaginal progestagen sponges impregnated with 30 mg of cronolone. After 12-14 days the sponges were removed, the females received an intramuscular injection …
