Immunodetection Of Insulin-Like Growth Factor Binding Proteins (Igfbps) In The Sera Of Different Animal Species,
2011
TÜBİTAK
Immunodetection Of Insulin-Like Growth Factor Binding Proteins (Igfbps) In The Sera Of Different Animal Species, Romana Masnikosa, Ivona Baricevic, Dragana Lagundzin, Olgica Nedic
Turkish Journal of Veterinary & Animal Sciences
Knowledge regarding the insulin-like growth factor (IGF) system and the methods for the detection and quantitation of IGF binding proteins (IGFBPs) is greater for the human species than for other animal species. This is mainly because antibodies specifically raised against nonhuman IGFBPs are rarely commercially available. We aimed to investigate which of the antibodies directed against human IGFBPs could be used for the detection of IGFBPs present in other species. We used commercially available antibodies, raised against human IGFBP-1 through IGFBP-4, in order to recognize the corresponding IGFBPs in the sera of different animal species: human, bovine, equine, porcine, ovine, …
Expression Of Plasma Prolactin And Pituitary Prolactin Mrna Around The Broody Cycle In Wan-Xi White Goose,
2011
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Expression Of Plasma Prolactin And Pituitary Prolactin Mrna Around The Broody Cycle In Wan-Xi White Goose, Runshen Jiang, Xingyong Chen, Ruhua Wei, Zhaoyu Geng
Turkish Journal of Veterinary & Animal Sciences
A systematic evaluation of the dynamic changes of prolactin (PRL) around the whole cycle of broodiness in domestic fowl has not been performed. We started to investigate the overall changing profile of PRL expression at both protein and mRNA levels from pre- to post-broodiness in Wan-xi White goose, an indigenous breed in East China. The onset of broodiness was recorded, and the plasma PRL concentration and the pituitary PRL mRNA level at 5 stages were measured, in that order, around the first broody cycle. The expression level of pituitary PRL mRNA was highly correlated with the plasma PRL level (r …
The Occipital Area In Medieval Dogs And The Role Of Occipital Dysplasia In Dog Breeding,
2011
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The Occipital Area In Medieval Dogs And The Role Of Occipital Dysplasia In Dog Breeding, Maciej Janeczek, Aleksander Chrószcz
Turkish Journal of Veterinary & Animal Sciences
An investigation was carried out on 42 dog skulls from the early medieval period. The skulls were excavated in Wroclaw, Poland, and in Novgorod and Moscow, Russia. Craniometric measurements were taken. On the basis of the basion-ethmoid measurement, the shoulder height was estimated. The foramen magnum height and width were measured and the foramen magnum index was calculated. The foramen magnum was typical in shape, and any occipital dysplasia signs observed were in the skulls. The results of the investigation, in comparison to other investigations of modern breeds and archaeozoological material, suggest that occipital dysplasia is not a pathology but …
Serological Study Of Leptospirosis Among Dairy Cattle In Bosnia And Herzegovina,
2011
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Serological Study Of Leptospirosis Among Dairy Cattle In Bosnia And Herzegovina, Maid Rifatbegovic, Zinka Maksimovic
Turkish Journal of Veterinary & Animal Sciences
A total of 75,206 blood serum samples from dairy cattle and quarantined heifers was collected between 2001 and 2007 and analyzed for bovine leptospirosis by the microscopic agglutination test (MAT). Serovar Hardjo was used for testing of all sera, with the addition of 2 or 3 randomly chosen other serovars (Grippotyphosa, Icterohaemorrhagiae, Pomona, Bataviae, Canicola, and/or Saxcoebing). From the total number of sera tested, 1197 (1.59%) were serologically positive, with a decreasing tendency over the years of research. The most prevalent serovar was Pomona (1.32%), followed by Hardjo (0.52%) and Grippotyphosa (0.37%). The differences among the studied regions in terms …
Molecular And Serological Evidence For Anaplasma Infection In Yaks,
2011
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Molecular And Serological Evidence For Anaplasma Infection In Yaks, Miling Ma, Zhijie Liu, Guiquan Guan, Youquan Li, Jianxun Luo, Hong Yin
Turkish Journal of Veterinary & Animal Sciences
Bovine anaplasmosis caused by Anaplasma marginale is considered to be an important disease in China; large-scale investigations have not been carried out since the 1990s, however, and whether or not yaks can be infected by A. marginale has never been investigated. In this study, 2 yaks were successfully infected by the inoculation of blood infected with A. marginale under experimental conditions. Furthermore, an investigation of 482 field serum samples from yaks using an enzyme-linked immunosorbent assay (ELISA) with the recombinant major surface protein 5 (MSP5) showed that 84.4% of the results were positive. In consideration of previously determined ecological and …
Maedi-Visna Virus Infection In A Merino Lamb With Nervous Signs,
2011
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Maedi-Visna Virus Infection In A Merino Lamb With Nervous Signs, Ahmet Akkoç, Meri̇ç Kocatürk, Ayli̇n Alasonyalilar Demi̇rer, Sezgi̇n Şentürk, Giacomo Renzoni, Silvia Preziuso
Turkish Journal of Veterinary & Animal Sciences
A Maedi-Visna virus (MVV) infection was diagnosed in a 10-month-old lamb from a Turkish flock. The animal suddenly showed nervous signs and died within 2 days. At necropsy, different pulmonary and nervous lesions were observed. A Coenurus cerebralis cyst was also present in the right-brain hemisphere. Severe nonpurulent meningoencephalitis and lymphoproliferative pneumonia were detected by histopathology. MVV proviral DNA was demonstrated by polymerase chain reaction (PCR) in formalin-fixed and paraffin-embedded samples collected from the brain and the lungs. This unusual report in a young animal confirms that not only adult sheep but also lambs can develop MVV disease. The hypothesis …
Congenital Cardiac Defect In A Pygmy Goat (Capra Hircus),
2011
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Congenital Cardiac Defect In A Pygmy Goat (Capra Hircus), Fulvio Laus, Ilenia Copponi, Matteo Cerquetella, Alessandro Fruganti
Turkish Journal of Veterinary & Animal Sciences
Congenital cardiac defects are anatomic conditions present at birth. Few references to such conditions in goats are available in the current scientific literature. This report describes, for the first time, a congenital cardiac disease clinically characterized by polypnea from birth and exercise intolerance in a 2-month-old pygmy goat. An atrioventricular dysplasia known as Ebstein's anomaly, an atrial septal defect, and a mild subaortic stenosis were ultrasonographically diagnosed.
Influence Of Pgf_{2\Alpha} On Semen Quality And Libido In Holstein Bulls,
2011
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Influence Of Pgf_{2\Alpha} On Semen Quality And Libido In Holstein Bulls, Reza Masoumi, Armin Towhidi, Ardsher N. Javaremi, Habib Nabizadeh, Mehdi Zhandi
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the effects of Cloprostenol (PGF_{2\alpha} analogue) on semen quality and libido in Holstein bulls. Ten low libido Iranian Holstein bulls were randomly allocated to 2 groups and received Cloprostenol (n = 5) or saline (n = 5) 30 min prior to the semen collection 2 days per week for 2 months. Reaction time was significantly decreased in the treatment group. Duration of ejaculation was significantly increased in the treatment group. Semen volume and sperm concentration were higher in treated bulls when compared with controls. The percentage of morphologically normal sperm cells, percentage …
Histological Examination Of Testicular Cell Development And Apoptosis In The Ostrich Chick,
2011
TÜBİTAK
Histological Examination Of Testicular Cell Development And Apoptosis In The Ostrich Chick, Wei Lan, Ke-Mei Peng, Huazhen Liu, Hui Song, Yang Wang, Li Tang
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to observe the microstructure and ultrastructure of the testis, demonstrate testicular cell development and apoptosis, and elucidate regularity of development and apoptosis in ostrich chick testes. By employing light microscopy 3 obvious development characteristics were detected with ostrich age increasing: first, many primordial germ cells and a few spermatogonia were found while seminiferous tubule integrity was not evident in testes of 1-day-old ostrich chicks nor were primordial germ cells. In the 30-day-old ostrich chicks spermatogonia were completely differentiated, but very few primary spermatocytes were observed in testes at 45 days of age. Second, the …
Stage-Specific Developmental Gene Expression Of Goat Preimplantation Embryos Produced In Vitro,
2011
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Stage-Specific Developmental Gene Expression Of Goat Preimplantation Embryos Produced In Vitro, Y. J. Li, H. Ao, G. J. Sun, L. Zhang
Turkish Journal of Veterinary & Animal Sciences
Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had …
Alteration Of Gene Expression In Microvascular Endothelial Cells After Exposure To Foot-And-Mouth Disease Virus 146s Antigen,
2011
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Alteration Of Gene Expression In Microvascular Endothelial Cells After Exposure To Foot-And-Mouth Disease Virus 146s Antigen, Tao Zhang, Shuang Zhang, Li-Fang Tian, Ge Hu, Zhan-Wei Suo, Wu Chen, Hui-Qin Duan, Xiang Mu, Ke-Dao Teng
Turkish Journal of Veterinary & Animal Sciences
To investigate the global response of microvascular endothelial cells (MVECs) to foot-and-mouth (FMDV)disease vaccine (FMDV) antigen and to explore the role of MVECs in FMD vaccine immunity, the gene expression alterations of rat myocardium microvascular endothelial cells (RMMVECs) after stimulation with FMD virus (FMDV) 146S antigen were assayed using cDNA microarrays. We observed that 18 genes and 67 genes in RMMVECs were respectively altered by FMDV 146S antigen at 6 h and 24 h, respectively. The former were all up-regulated genes, and the latter included 60 up-regulated genes and 7 down-regulated genes. Among the differentially expressed genes, many were associated …
Diet Preference And Breeding Success In Captive-Bred Greater Rheas (Rhea Americana): A Preliminary Study,
2011
TÜBİTAK
Diet Preference And Breeding Success In Captive-Bred Greater Rheas (Rhea Americana): A Preliminary Study, Gisela Bazzano, Joaquin. L. Navarro, Monica. B. Martella
Turkish Journal of Veterinary & Animal Sciences
We conducted 2 consecutive experiments to verify whether adult captive Greater rheas (Rhea americana) could select diets differing in nutritional and energetic content, and to evaluate the effect of those diets on egg production and hatchability. From August to January, animals were offered four diets: processed feed for rheas (diet 1), diet 1 + soybean (diet 2), processed feed for chicken (diet 3), and diet 3 + soybean (diet 4). Rheas preferred the diets containing feed for chicken (diets 3 and 4, 179,67 ± 12.12 [S.E.] g/ind./day). Diet 2 was more consumed (95.67 ± 16.70 g/ind./day) than diet 1 (60.09 …
Association Between Polymorphisms Of Cyp19, Cyp21, And Er1 Genes And Milk Production Traits In Black-And-White Cattle,
2011
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Association Between Polymorphisms Of Cyp19, Cyp21, And Er1 Genes And Milk Production Traits In Black-And-White Cattle, Magdalena Jedrzejczak, Wilhelm Grzesiak, Iwona Szatkowska, Andrzej Dybus, Magdalena Muszynska, Daniel Zaborski
Turkish Journal of Veterinary & Animal Sciences
The relationships between the SNPs of the cytochrome P450 gene (CYP19/PvuII), steroid 21-hydroxylase (P450c21) gene (CYP21/HpaII), 2 polymorphic sites of estrogen receptor alpha gene (ER1/BglI and ER1/SnaBI), and milk production traits of Black-and-White cattle were analyzed. A total of 472 cows were included in the study and genotyped using PCR-RFLP. The frequencies of alleles for the Black-and-White cows were as follows: 0.923 - CYP19A, 0.077 - CYP19B, 0.042 - ER1/BglIA, and 0.958 for ER1/BglIG. For ER1/SnaBI, the frequency of allele A was 0.960 and that of allele G was 0.040. In the CYP21, all cows were genotyped as AA (no …
Haematology Of Downer Dairy Cows With Fatty Liver,
2011
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Haematology Of Downer Dairy Cows With Fatty Liver, Emmanouil Kalaitzakis, Nikolaos Panousis, Nikolaos Roubies, Eleni Kaldrymidoy, Harilaos Karatzias
Turkish Journal of Veterinary & Animal Sciences
This study examined downer dairy cows with concurrent fatty liver of different degrees and evaluated the haematological values, the relationship and joint occurrence of fatty liver severity and haematology and compared the haematological values in the downer animals that finally died or cured. Blood and liver biopsy samples were obtained from 36 Holstein downer cows shortly after they became recumbent and before they were treated. Blood analysis included determination of haematocrit, haemoglobin, white blood, granular cell and lymphocytes count, and platelet number. Liver tissue was examined histologically and was classified according to severity of fatty liver. The majority of downer …
Aflatoxin M1 Levels In Retail Yoghurt And Ayran In Erzurum In Turkey,
2011
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Aflatoxin M1 Levels In Retail Yoghurt And Ayran In Erzurum In Turkey, Meryem Aydemi̇r Atasever, Mustafa Atasever, Korhan Özturan
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to determine the presence and levels of aflatoxin M1 (AFM1) in yoghurt and ayran consumed in the province of Erzurum, Turkey. For this purpose, a total of 80 yoghurt samples and 80 ayran samples were randomly obtained from markets. The samples' AFM1 content and concentrations were examined by competitive ELISA method. AFM1 at detectable level (5 ng/kg) was found in 87.5% of the yoghurt samples and in 90.0% of the ayran samples. AFM1 levels in 16 (20%) yoghurt and in 11 (13.6%) ayran samples exceeded the maximum tolerable limit of the Turkish Food Codex. The …
Association Analysis Of Aquaporin 7 (Aqp7) Gene Variants With Semen Quality And Fertility In Bulls,
2011
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Association Analysis Of Aquaporin 7 (Aqp7) Gene Variants With Semen Quality And Fertility In Bulls, Tenghe Ma, Jifeng Liu, Ruifeng Zhao, Hao Jiang, Lisheng Dai, Yumin Zhao, Zhihui Zhao, Jiabao Zhang
Turkish Journal of Veterinary & Animal Sciences
Aquaporin 7 (AQP7) gene as a candidate Antifreeze gene was investigated and associated with fresh and frozen semen quality traits in 45 Simmental and Charolais bulls. PCR-SSCP, PCR-RFLP, and sequencing method were employed to detect the single nucleotide polymorphisms (SNPs) of the AQP7 gene. Two mutations, A264G and G371C, were identified. The allele frequencies were A (0.97, 0.96) to B (0.03, 0.04) and C (0.97, 0.91) to D (0.03, 0.09) in Simmental and Charolais bulls, respectively. A264G substitution was significantly associated with acrosome integrity ratio (P < 0.01) and motility (P < 0.05) in frozen semen. Meanwhile, G371C substitution was significantly associated with acrosome integrity ratio (P < 0.05), percentages of viable sperm (P < 0.05), and motility (P < 0.01) in frozen semen. The results prove that AQP7 gene SNPs are useful for marker-assisted selection in early seed selection in the bull industry.
Effects Of Varying Levels Of Protected Fat On Performance Of Shami Goats During Early And Mid Lactation,
2011
TÜBİTAK
Effects Of Varying Levels Of Protected Fat On Performance Of Shami Goats During Early And Mid Lactation, Hosam Titi
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the responses of early lactating Shami goats to varying amounts of dietary supplementation of Ca salts of fatty acids. Shami goats (n = 75) in their first week of lactation were randomly assigned to 3 isonitrogenous dietary (TMR) treatments containing 0%, 3%, or 5% added Ca salt. The study took 60 days during which the following variables were measured: milk production and composition, body weight, feed intake, serum cholesterol and triglyceride, kids weaning weight, and milk fatty acids. Milk production and energy corrected milk were higher (P < 0.05) at 5% level. Percentages of milk composition were higher (P < 0.05) at 3% with CP% not different from 5%. No differences were observed in yields of different milk components including casein, final body weights, feed intake, or weaning weights of kids. Intake of metabolizable energy was higher (P < 0.05) at 5% level of Ca salts. Differences in milk fatty acids were mainly restricted to unsaturated ones with no effect on the saturated fatty acids. Such results suggest that milk production and content of lactating Shami goat can be changed by a level of 5% of Ca salts.
Analysis Of The Orf Region Of The Prion Protein Gene In The Botosani Karakul Sheep Breed From Romania,
2011
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Analysis Of The Orf Region Of The Prion Protein Gene In The Botosani Karakul Sheep Breed From Romania, Steliana Elvira Maria Kevorkian, Mihaela Zaulet, Maria Adina Manea, Sergiu Emil Georgescu, Marieta Costache
Turkish Journal of Veterinary & Animal Sciences
Ovine scrapie is considered a neurodegenerative disease characterized by the accumulation of an abnormal, protease-resistant isoform of PrP. The polymorphisms at codons 136, 154, and 171 are associated with susceptibility to scrapie. In order to detect the existing polymorphisms at the 3 codons we used the real-time PCR technique. The obtained genotypes were confirmed by PCR amplification and sequencing of a 402 bp fragment of exon 3 of the prion gene. Known polymorphisms were detected at codons 136, 154, and 171 and 5 different genotypes were determined. The results showed that all animals were homozygous for A at codon 136 …
The Effect Of Ovine Placenta Extract, On Mammogenesis, Lactogenesis And Galactopoiesis In Sheep,
2011
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The Effect Of Ovine Placenta Extract, On Mammogenesis, Lactogenesis And Galactopoiesis In Sheep, Gabriel Cotor, Aneta Pop, Marian Ghita
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to evaluate the effect of ovine placenta extract, obtained by affinity chromatography on immobilized wheat germ lectin, on mammogenesis, lactogenesis, and galactopoiesis in pregnant and lactating sheep. Placental extract (100 \mug/mL protein concentration) was subcutaneously injected to pregnant sheep in days 1, 4, 7, 10, and 13 of the experiment (totally 10 mL placenta extract/animal) alternatively on the right and left side of the neck. Placental extract induced a more intense development of udder parameters; udder circumference increase was of 16.73%, anteroposterior length increase was of 15.9%, and latero-lateral length of 11.1%. Placental …
Acetic Acid Modulates Induction Of Pulmonary Hypertension In Broiler Chickens: Based On Electrocardiographic Parameters,
2011
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Acetic Acid Modulates Induction Of Pulmonary Hypertension In Broiler Chickens: Based On Electrocardiographic Parameters, Hossein Hassanpour, Abdolkarim Zamani Moghadam, Hamed Zarei, Neda Taghavi
Turkish Journal of Veterinary & Animal Sciences
To clarify the effect of acetic acid on the electrocardiographic parameters of pulmonary hypertensive broilers, chicks were reared at high altitude and treated with acetic acid (0.4 and 0.9 g/L) for 45 days. The right ventricle-to-total ventricle ratio was noted as an index of pulmonary hypertension that was significantly (P < 0.05) decreased in the treated groups. S amplitudes were significantly decreased only at 36 days (leads II and III) and 45 days (lead aVF) in many treated groups. There were significant (P < 0.05) reductions of T amplitudes at 28 days (leads aVR and aVL) and 36 days (lead aVL), and significant elevations of QRS (leads II, III, and aVF) and QT intervals (leads II, aVR, aVL, and aVF) at different ages of many treated groups. R amplitudes were significant (P < 0.05) at 28 days (leads aVL and aVF, at both doses of acetic acid), 36 days (leads aVR and aVL, at 0.4 g/L of acetic acid), and 45 days (lead aVR, at both doses of acetic acid). The RR interval also significantly increased at 28 days (leads aVR and aVL) and 45 days (lead aVL). It was concluded that acetic acid modulates induction of ventricular hypertrophy, dilation, and arrhythmia. These effects are detectable in many electrocardiographic parameters.
