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Presence Of Enterotoxin And Verotoxin In Turkish Cheeses Sold In İstanbul, ENVER BARIŞ BİNGÖL, ÖMER ÇETİN, HİLAL ÇOLAK, HAMPARSUN HAMPİKYAN 2012 TÜBİTAK

Presence Of Enterotoxin And Verotoxin In Turkish Cheeses Sold In İstanbul, Enver Bariş Bi̇ngöl, Ömer Çeti̇n, Hi̇lal Çolak, Hamparsun Hampi̇kyan

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to determine the presence of enterotoxin and verotoxin levels in Turkish cheeses sold in retail stores in İstanbul, Turkey. For this purpose, 150 cheese samples (25 pieces of each: white pickled cheese, tulum cheese, mihaliç cheese, hellim cheese, örgü cheese, and civil cheese) were analyzed microbiologically for Staphylococcus aureus, Escherichia coli, and E. coli O157:H7, and the presence of the toxins was identified using an ELISA test (RIDASCREEN® Set A, B, C, D, E and Verotoxin). Of the examined samples, 40 (26.66%) were positive for S. aureus and 55 (36.66%) were positive for E. coli, …


Serum Lysozyme Concentrations In Broilers Treated With Sel-Plex® And Sodium Selenite And Infected With Еimeria Tenella, VETSISLAV KOINARSKI, LILIAN K. SOTIROV, STEFAN DENEV 2012 TÜBİTAK

Serum Lysozyme Concentrations In Broilers Treated With Sel-Plex® And Sodium Selenite And Infected With Еimeria Tenella, Vetsislav Koinarski, Lilian K. Sotirov, Stefan Denev

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to elucidate the potential role of humoral factors of innate immunity in broilers infected with Eimeria tenella under the influence of inorganic and organic Se cations. It was found that the chickens treated with Sel-Plex® had higher serum lysozyme concentrations compared to those treated with sodium selenite in infected and noninfected groups. The authors consider that Sel-Plex® has a better expressed immunostimulating effect on serum lysozyme concentrations than sodium selenite.


Protective Effect Of N-Acetyl-L-Cysteine Against Acrylamide-Induced Oxidative Stress In Rats, EBRU IŞIK ALTURFAN, AYFER BECEREN, AHMET ÖZER ŞEHİRLİ, ZEYNEP EMEL DEMİRALP, GÖKSEL ŞENER, GÜLDEN ZEHRA OMURTAG 2012 TÜBİTAK

Protective Effect Of N-Acetyl-L-Cysteine Against Acrylamide-Induced Oxidative Stress In Rats, Ebru Işik Alturfan, Ayfer Beceren, Ahmet Özer Şehi̇rli̇, Zeynep Emel Demi̇ralp, Göksel Şener, Gülden Zehra Omurtag

Turkish Journal of Veterinary & Animal Sciences

Acrylamide (AA), used in many fields, from industrial manufacturing to laboratory work, is also formed during the heating process through the interactions of amino acids. Therefore, AA poses a significant risk for both human and animal health. This study aimed to elucidate whether N-acetyl-L-cysteine (NAC) treatment could modulate AA-induced oxidative changes in the brain, lung, liver, kidney, and testes tissues of the rat. Rats were divided into 4 groups, as the control (C), NAC [150 mg/kg intraperitoneally (i.p.)], AA (40 mg/kg i.p.), and NAC + AA groups. After 10 days, the rats were decapitated and the tissues were excised. Malondialdehyde …


Pregnancy Rate Following Artificial Insemination Or Natural Service In Postpartum Estrus Synchronized Beef Cattle, ABDUL MALIK, ABD WAHID HARON, ROSNINA YUSOFF, AZHAR KASIM, SABRI MOHD YUSOFF 2012 TÜBİTAK

Pregnancy Rate Following Artificial Insemination Or Natural Service In Postpartum Estrus Synchronized Beef Cattle, Abdul Malik, Abd Wahid Haron, Rosnina Yusoff, Azhar Kasim, Sabri Mohd Yusoff

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the pregnancy rate following artificial insemination (AI) or natural service (NS) in postpartum estrous synchronized beef cattle. A total of 85 Brangus cows, postpartum, were divided into 2 groups. Group 1 consisted of 35 cows and was further subdivided into 7 groups, each consisted of 5 cows that were naturally mated. Group 2 consisted of 40 cows that were artificially inseminated. Estrus synchronization was carried out using a controlled internal drug releasing device (CIDR) containing 1.38 mg of progesterone, for 7 days. Group 1 cows were mixed with bulls at a 1:5 …


Ectoparasites On Roe Deer (Capreolus Capreolus) In Samsun, Turkey, MUSTAFA AÇICI, CENK SONER BÖLÜKBAŞ, YUNUS EMRE BEYHAN, GÖKMEN ZAFER PEKMEZCİ, ALİ TÜMAY GÜRLER, ŞİNASİ UMUR 2012 TÜBİTAK

Ectoparasites On Roe Deer (Capreolus Capreolus) In Samsun, Turkey, Mustafa Açici, Cenk Soner Bölükbaş, Yunus Emre Beyhan, Gökmen Zafer Pekmezci̇, Ali̇ Tümay Gürler, Şi̇nasi̇ Umur

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to identify ectoparasites of wild roe deer (Capreolus capreolus) living in natural areas of Samsun Province that were wounded by car accidents or hunters between 2007 and 2010. A total of 90 ticks, 60 lice, and 5 deer keds were collected from 17 animals. Five tick species (Ixodes ricinus, Rhipicephalus bursa, Rhipicephalus turanicus, Haemaphysalis punctata, and Haemaphysalis concinna), 1 louse species (Cervicola meyeri), and 1 deer ked species (Lipoptena cervi) were identified. Cervicola meyeri and L. cervi were identified for the first time in Turkey.


Laparoscopic Paraovarian Cyst Removal In A Female Rhesus Macaque, VIJAY KUMAR, ANSHU RAJ, SAMEER RASTOGI 2012 TÜBİTAK

Laparoscopic Paraovarian Cyst Removal In A Female Rhesus Macaque, Vijay Kumar, Anshu Raj, Sameer Rastogi

Turkish Journal of Veterinary & Animal Sciences

A 15-year-old female rhesus macaque was showing signs of bleeding from the vulva, while no other signs were noted. The animal was treated symptomatically with no improvement. On abdominal laparoscopy, a small transparent growth was discovered on both of the uterine horns, between the ovaries and fallopian tubes, and after thorough examination was diagnosed as a paraovarian cyst. The left cyst was larger (5-6 cm) than the right cyst (3-4 cm). Both of the cysts were smooth and transparent due to accumulated fluid. The cysts were aspirated and removed by bipolar laparoscopy cauterization.


Genotypic Variation In The Brucella Melitensis Hemagglutinin Gene In Vaccine Strains And Field Isolates In Palestine, ELENA AWWAD, MOHAMMAD A. FARRAJ, KAMEL ADWAN, TAMER E. ESSAWI 2012 TÜBİTAK

Genotypic Variation In The Brucella Melitensis Hemagglutinin Gene In Vaccine Strains And Field Isolates In Palestine, Elena Awwad, Mohammad A. Farraj, Kamel Adwan, Tamer E. Essawi

Turkish Journal of Veterinary & Animal Sciences

In Palestine, as in other countries, brucellosis is an endemic disease, even in highly vaccinated zones. Many reports suggest that a decline in vaccine productivity may be due to antigenic shifts in the circulating Brucella melitensis. To address this aspect, the hemagglutinin gene from B. melitensis field isolates was amplified by polymerase chain reaction (PCR), sequenced, digested with the EcoRV and HaeIII, and compared to the Rev.1 strain of the B. melitensis vaccine. From January to December 2008, 80 milk samples were collected from infected flocks, from different West Bank regions of Palestine. From these, 77.5% (62/80) were shown to …


Avian Influenza Virus In Migratory And Resident Birds During Migratory Season In Boushehr, Iran, MOHAMMAD JAVAD MEHRABANPOUR, ABDOLAH RAHIMIAN, ALI SHIRAZINEZAD, HOSSEIN MOEIN, MOHAMMAD AMIN SHAYANFAR 2012 TÜBİTAK

Avian Influenza Virus In Migratory And Resident Birds During Migratory Season In Boushehr, Iran, Mohammad Javad Mehrabanpour, Abdolah Rahimian, Ali Shirazinezad, Hossein Moein, Mohammad Amin Shayanfar

Turkish Journal of Veterinary & Animal Sciences

Avian influenza (AI) is an infectious disease of birds caused by type A strains of the influenza virus. Among these AI viruses, only serotypes H5 and H7 are considered highly pathogenic in poultry. However, serotype H9N2 has also been found to produce severe respiratory tract infections in chickens. Migratory birds of the world are a natural reservoir of influenza viruses of all subtypes. This is the first report of isolation and molecular survey of AI virus from migratory and wild resident birds in Boushehr, Iran. These areas are at risk for the spread and transmission of influenza A viruses. A …


Comparative Histological Structure Of The Sinus Node In Mammals, ABOLGHASEM NABIPOUR 2012 TÜBİTAK

Comparative Histological Structure Of The Sinus Node In Mammals, Abolghasem Nabipour

Turkish Journal of Veterinary & Animal Sciences

The main function of the sinus node is to provide the electrical impulse responsible for normal cardiac rhythm. The rhythmic coordination depends on the passage of the excitation wave by the heart through its conductive system of impulse. There is considerable variability in the size and exact location of the sinus node of individual hearts even within the same species. Histological features of the sinus node including the cells, the nodal artery, the collagen framework, and the innervation, and also some physiological characteristics of the node, will be discussed.


Neuronal And Extraneuronal Lipofuscinosis In Merino Sheep Grazing Asphodelus Aestivus Seeds In Western Turkey, SÜMBÜL SERAP BİRİNCİOĞLU, WOLFGANG SCHMAHL, HAMDİ AVCI 2012 TÜBİTAK

Neuronal And Extraneuronal Lipofuscinosis In Merino Sheep Grazing Asphodelus Aestivus Seeds In Western Turkey, Sümbül Serap Bi̇ri̇nci̇oğlu, Wolfgang Schmahl, Hamdi̇ Avci

Turkish Journal of Veterinary & Animal Sciences

Neuronal and extraneuronal lipofuscinosis was determined in Merino sheep (6 sheep and 3 heads of sheep) of 2-3 years old that had grazed on Asphodelus aestivus seeds. Clinically, the sheep exhibited tremors, paresis, oral and nasal discharge, and severe respiratory distress. Haemorrhagic necrotic tracheitis, aspiration pneumonia, and subacute enteritis were observed in 6 sheep during macroscopic examinations. The brains of the animals were of firm consistency and yellowish brown in colour. Histological examination of the central nervous system revealed that most of the cytoplasm of neurons contained yellowish brown pigment granules. Pigmentations were particularly detected in the pons, medulla oblongata, …


Biotin Affects The Immune Response Of Piglets Inoculated With Porcine Circovirus Type 2, CHEN HONG, ZHANG KEYING, DING XUEMEI, CHEN DAIWEN 2012 TÜBİTAK

Biotin Affects The Immune Response Of Piglets Inoculated With Porcine Circovirus Type 2, Chen Hong, Zhang Keying, Ding Xuemei, Chen Daiwen

Turkish Journal of Veterinary & Animal Sciences

Weanling crossbred pigs were used in a 5-week trial to evaluate the effects of biotin and porcine circovirus type 2 (PCV2) challenge on serum cytokine concentrations and humoral immunoresponse. Although not significant, there was a trend towards reduced interferon-\gamma concentration in the challenged pigs compared to the controls, especially at 14 and 21 days postinoculation (dpi). Biotin supplementation with 50 or 200 µg/kg biotin improved these levels, with higher doses producing an earlier and stronger response. Although the magnitude of the immune response was small and inconsistent, diet supplementation with 50 or 200 µg/kg biotin appeared to increase serum immunoglobulin …


Effect Of Aloe-Plus Preparation Supplement On Hematological And Immunological Blood Parameters And Performance Of Turkey Hens, KATARZYNA OGNIK, IWONA SEMBRATOWICZ 2012 TÜBİTAK

Effect Of Aloe-Plus Preparation Supplement On Hematological And Immunological Blood Parameters And Performance Of Turkey Hens, Katarzyna Ognik, Iwona Sembratowicz

Turkish Journal of Veterinary & Animal Sciences

It has long been considered that aloe has many beneficial effects for humans and animals, exhibiting antibiotic, antihistaminic, anesthetic, antiinflammatory, and immunostimulant properties. The aim of the present study was to evaluate the influence of supplemental aloe preparation on some immunological and hematological indices of turkey hen blood. The study involved 160 medium-heavy BUT-9-type 6-week-old female turkey hens, administered Aloe-plus plant preparation at the following varying concentrations: Group 1, control without aloe addition; Group 2, daily doses of 0.35 mL/kg body weight; Group 3, daily doses of 0.70 mL/kg body weight; and Group 4, daily doses of 1.40 mL/kg body …


Equidae Milk Promises Substitutes For Cow And Human Breast Milk, AKBAR NIKKHAH 2012 TÜBİTAK

Equidae Milk Promises Substitutes For Cow And Human Breast Milk, Akbar Nikkhah

Turkish Journal of Veterinary & Animal Sciences

Milk is the most nourishing natural biofluid. Milk bioactives contribute significantly to meeting nutrient requirements while minimizing the risks of cancers, traumas, and metabolic complexities. Some mothers may not be able to feed their infants breast milk for a prolonged period. Very young infants and the elderly may be intolerant to cow milk and its proteins and cholesterol. Thus, viable substitutes are needed to complement human breast milk in such cases. This review describes the most significant research findings to date and highlights the special nutritional and health properties of donkey and mare milks as promising substitutes for cow and …


Growth Performance And Intestinal Histomorphology In Egg-Type Growing Roosters Fed Recycled Food Waste Containing Effective Microorganisms, JASSADA RUTTANAVUT, KOH EN YAMAUCHI 2012 TÜBİTAK

Growth Performance And Intestinal Histomorphology In Egg-Type Growing Roosters Fed Recycled Food Waste Containing Effective Microorganisms, Jassada Ruttanavut, Koh En Yamauchi

Turkish Journal of Veterinary & Animal Sciences

To evaluate the effects of a recycled food waste (eco-feed) containing effective microorganisms derived from Japanese mugwort silage juice (EJP) on growth performance and intestinal histomorphology, 48 male chickens of 14 days old were divided into 0%, 20%, 40%, and 60% EJP diet groups. At 20 weeks of age, body weight gain was similar in the 20% EJP group but lower in the 40% and 60% EJP groups (P < 0.05) compared to that in the control group. The relative total intestinal length and weight tended to be long and heavy, respectively, in the 20% and 40% EJP groups, but short and light, respectively, in the 60% EJP group. Although numerous villi were found in the jejunum and ileum of all EJP groups, the villus height and size observed in these groups were smaller. Cell area and cell mitosis number in the duodenum and jejunum of all EJP groups were lower than those of the control group (P < 0.01). Epithelial cells on the villus apical surface of the 20% EJP group showed morphology similar to that of cells on the villus apical surface of the control group in all of the intestinal segments. The growth performance data suggested that EJP could be incorporated at up to a level of 20% in chicken diets. The intestinal histological results suggested that long-term feeding of an EJP diet at up to the 40% level may result in a long and heavy intestine by increasing the numbers of miniature intestinal villi.


Determination Of Acute Phase Proteins After Experimental Streptococcus Iniae Infection In Tilapia (Oreochromis Niloticus L.), AZİME KÜÇÜKGÜL GÜLEÇ, İBRAHİM CENGİZLER 2012 TÜBİTAK

Determination Of Acute Phase Proteins After Experimental Streptococcus Iniae Infection In Tilapia (Oreochromis Niloticus L.), Azi̇me Küçükgül Güleç, İbrahi̇m Cengi̇zler

Turkish Journal of Veterinary & Animal Sciences

Tilapia (Oreochromis niloticus Linnaeus, 1758) were infected with Streptococcus iniae (Cat No: ATCC 29178) and the plasma changes in the acute phase protein (APP) members were examined at different time periods. A total of 72 fish with average body weights of 60 g were used. Fish in the infected group (group I) were intraperitoneally injected with S. iniae at 3.3 × 10^5 colony forming unit (CFU) in 0.1 mL level to the fish. The other group (group II) was subjected to handling stress near the infection. The control group was constituted by providing optimum conditions. On days 7, 14, and …


An Immunohistochemical Study On The Distribution Of Endocrine Cells In The Digestive Tract Of Gray Goose (Anser Anser), JUN YANG, LEI ZHANG, XIN LI, LEII ZHANG, XIANGJIANG LIU, KEMEI PENG 2012 TÜBİTAK

An Immunohistochemical Study On The Distribution Of Endocrine Cells In The Digestive Tract Of Gray Goose (Anser Anser), Jun Yang, Lei Zhang, Xin Li, Leii Zhang, Xiangjiang Liu, Kemei Peng

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the morphology and the distribution of 5-hydroxytryptamine (5-HT), somatostatin (SS), gastrin (Gas), glucagon (Glu), and substance P immunoreactive (IR) cells in the digestive tract of gray goose by the immunohistochemical streptavidin-peroxidase method. The samples were taken from 10 healthy adult gray geese. The results showed that 5 kinds of IR cells were mainly distributed between the mucous epithelium and intestinal gland. The number of 5-HT-IR cells was highest in the rectum and duodenum, but none were observed in the pylorus. SS-IR cells appeared in great numbers in the pylorus, duodenum, and cecum; …


Changes In Cell Wall Compositions And Degradation Kinetics Of Electron Beam-Irradiated Sugarcane Bagasse, PARVIN SHAWRANG, ABBAS MAJDABADI, ALI ASGHAR SADEGHI 2012 TÜBİTAK

Changes In Cell Wall Compositions And Degradation Kinetics Of Electron Beam-Irradiated Sugarcane Bagasse, Parvin Shawrang, Abbas Majdabadi, Ali Asghar Sadeghi

Turkish Journal of Veterinary & Animal Sciences

Electron beam irradiation of sugarcane bagasse at doses of 250 and 500 kGy could change its cell wall composition and ruminal fiber degradation characteristics by breaking off the lignocellulosic bonds. Nylon bags of untreated or electron beam-irradiated bagasse were suspended in the rumen of 3 fistulated rams for up to 72 h, and the resulting data were fitted to a nonlinear degradation model to calculate the degradation parameters of dry matter (DM) and neutral detergent fiber (NDF). Irradiation had no effect on crude protein, ether extract, and ash, but resulted in a linear decrease of the cell wall contents. An …


Changes In Carbonic Anhydrase Activity And Gene Expression Of Hsp70 In Rainbow Trout (Oncorhynchus Mykiss) Muscle After Exposure To Some Metals, HAKAN SÖYÜT, ŞÜKRÜ BEYDEMİR, SALTUK BUĞRAHAN CEYHUN, ORHAN ERDOĞAN, ELİF DUYGU KAYA 2012 TÜBİTAK

Changes In Carbonic Anhydrase Activity And Gene Expression Of Hsp70 In Rainbow Trout (Oncorhynchus Mykiss) Muscle After Exposure To Some Metals, Hakan Söyüt, Şükrü Beydemi̇r, Saltuk Buğrahan Ceyhun, Orhan Erdoğan, Eli̇f Duygu Kaya

Turkish Journal of Veterinary & Animal Sciences

The effects of some heavy metals Co(II), Cu(II), Zn(II), and Ag(I) on carbonic anhydrase (CA) activity from rainbow trout (RT) muscle were investigated. Moreover, the effects of these metals on the expression of heat shock protein 70 (Hsp70) gene in muscle tissue were examined by real-time quantitative PCR (RT-PCR) in muscle tissue after exposure to the metals at the end of 6, 12, 24, and 48 h. CA was purified with a specific activity of 2300 EU mg^{-1}, a yield of 19%, and 1080-fold. The molecular weights (Mw) of subunit and native enzyme were approximately 30 and 31 kDa, respectively. …


Purification And Characterization Of Glutathione Reductase From Turkey Liver, PINAR TAŞER, MEHMET ÇİFTCİ 2012 TÜBİTAK

Purification And Characterization Of Glutathione Reductase From Turkey Liver, Pinar Taşer, Mehmet Çi̇ftci̇

Turkish Journal of Veterinary & Animal Sciences

This study aimed to purify glutathione reductase (GR) from turkey liver and investigate some of its characteristic features. The purification procedure comprised 2 steps: homogenate preparation and 2',5'-ADP Sepharose 4B affinity gel chromatography. Thanks to the 2 consecutive procedures, the enzyme, having the specific activity of 606.67 EU mg protein^{-1}, was purified with a yield of 10.75% and 2476-fold, and, in order to control enzyme purity, SDS-PAGE was done. Optimal pH, stable pH, optimal temperature, optimal ionic strength, molecular weight, turnover number of enzyme, and k_{cat}/K_M and K_M and V_{max} values for NADPH and glutathione disulfide substrates were also determined …


Prevalence And Factors Affecting The Presence Of Campylobacter Spp. In Broiler Carcasses In Bulgaria, HRISTO DASKALOV, ALEXANDER MARAMSKI 2012 TÜBİTAK

Prevalence And Factors Affecting The Presence Of Campylobacter Spp. In Broiler Carcasses In Bulgaria, Hristo Daskalov, Alexander Maramski

Turkish Journal of Veterinary & Animal Sciences

In an 11-month study, 292 samples from 13 slaughterhouses in Bulgaria were investigated for the presence of Campylobacter spp., and some factors affecting Campylobacter prevalence were analyzed. The study revealed that 44.9% of the samples were positive for Campylobacter spp. The main species detected were C. coli and C. jejuni. There was also one confirmed C. lari isolate. The C. coli strains prevailed over C. jejuni at 61.8% and 37.4%, respectively. A seasonal variation in the Campylobacter spp. presence in slaughter broiler carcasses was found with a predominance of positive isolates during June and July 2008. The conventional type of …


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